SitesBLAST
Comparing WP_020177398.1 NCBI__GCF_000385335.1:WP_020177398.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P0A7B5 Glutamate 5-kinase; Gamma-glutamyl kinase; GK; EC 2.7.2.11 from Escherichia coli (strain K12) (see paper)
41% identity, 93% coverage: 26:384/388 of query aligns to 7:365/367 of P0A7B5
- S50 (= S70) binding substrate
- D137 (= D157) binding substrate
- N149 (= N169) binding substrate
2j5tD Glutamate 5-kinase from escherichia coli complexed with glutamate (see paper)
41% identity, 93% coverage: 26:384/388 of query aligns to 5:363/365 of 2j5tD
2j5vB Glutamate 5-kinase from escherichia coli complexed with glutamyl-5- phosphate and pyroglutamic acid (see paper)
37% identity, 93% coverage: 26:384/388 of query aligns to 5:323/325 of 2j5vB
- binding pyroglutamic acid: T11 (≠ S32), G49 (= G71), A50 (≠ S72), I51 (≠ V73), A52 (= A74), D135 (= D157)
- binding gamma-glutamyl phosphate: K8 (= K29), S48 (= S70), D135 (= D157), G145 (= G167), D146 (= D168), N147 (= N169)
2j5vA Glutamate 5-kinase from escherichia coli complexed with glutamyl-5- phosphate and pyroglutamic acid (see paper)
37% identity, 93% coverage: 26:384/388 of query aligns to 5:321/323 of 2j5vA
- binding magnesium ion: K8 (= K29), G10 (= G31), L166 (= L188)
- binding pyroglutamic acid: T11 (≠ S32), S48 (= S70), G49 (= G71), A50 (≠ S72), I51 (≠ V73)
- binding gamma-glutamyl phosphate: K8 (= K29), G10 (= G31), S48 (= S70), D135 (= D157), D146 (= D168), N147 (= N169)
8j0gB Gk monomer complexes with glutamate and atp
36% identity, 62% coverage: 24:265/388 of query aligns to 8:257/274 of 8j0gB
- binding adenosine-5'-triphosphate: K13 (= K29), G15 (= G31), T16 (≠ S32), A17 (≠ S33), V185 (= V191), G191 (≠ A197), P193 (= P199), F215 (≠ G223), R223 (= R231), G225 (= G233), M226 (= M234), K229 (= K237)
- binding gamma-l-glutamic acid: A55 (≠ S72), N147 (= N154), D150 (= D157), N163 (= N169), R223 (= R231)
- binding magnesium ion: R223 (= R231), G224 (= G232), G225 (= G233)
- binding : Y65 (vs. gap), L68 (vs. gap), V69 (vs. gap), F73 (vs. gap)
8j0eB Gk monomer complexes with catalytic intermediate
36% identity, 62% coverage: 24:265/388 of query aligns to 11:252/269 of 8j0eB
- binding magnesium ion: D163 (= D170), G219 (= G232)
- binding (2~{R})-5-[[[[(2~{R},3~{S},4~{S},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-2-azanyl-5-oxidanylidene-pentanoic acid: K16 (= K29), G18 (= G31), T19 (≠ S32), A20 (≠ S33), S56 (= S70), G57 (= G71), V59 (= V73), D153 (= D157), N162 (= N169), V184 (= V191), P192 (= P199), K217 (≠ S230), R218 (= R231), G219 (= G232), G220 (= G233), M221 (= M234)
- binding : Y68 (vs. gap), L71 (vs. gap), V72 (vs. gap), V72 (vs. gap), S74 (vs. gap), F76 (vs. gap), F76 (vs. gap), Q80 (≠ P83)
2akoA Crystal structure of glutamate 5-kinase from campylobacter jejuni
33% identity, 60% coverage: 24:256/388 of query aligns to 1:219/241 of 2akoA
- binding adenosine-5'-diphosphate: H10 (≠ S33), S161 (= S189), D162 (= D190), I163 (≠ V191), F166 (≠ L194), Y167 (= Y195), N170 (≠ P198), P171 (= P199), T194 (≠ R231), G196 (= G233), K200 (= K237)
8j0fA Gk tetramer with adjacent hooks at reaction state (see paper)
36% identity, 62% coverage: 24:265/388 of query aligns to 12:251/270 of 8j0fA
- binding adenosine-5'-diphosphate: A21 (≠ S33), D183 (= D190), V184 (= V191), Y188 (= Y195), G190 (≠ A197), F214 (≠ A222), G219 (= G233), M220 (= M234)
- binding magnesium ion: R217 (= R231), G218 (= G232), G219 (= G233)
- binding gamma-glutamyl phosphate: K17 (= K29), T20 (≠ S32), S57 (= S70), D154 (= D157), N162 (= N169), R217 (= R231)
- binding : L72 (vs. gap), V73 (vs. gap), N74 (vs. gap), S75 (vs. gap), S76 (vs. gap), F77 (vs. gap), F77 (vs. gap), A78 (vs. gap), L80 (= L82)
7wx3B Gk domain of drosophila p5cs filament with glutamate, atp, and NADPH (see paper)
33% identity, 64% coverage: 19:265/388 of query aligns to 8:246/258 of 7wx3B
- binding gamma-glutamyl phosphate: S59 (= S70), A61 (≠ S72), N149 (= N154), T150 (≠ E155), D152 (= D157), M158 (≠ E163), N165 (= N169)
- binding : E72 (≠ P83), M75 (vs. gap), L77 (vs. gap), S78 (vs. gap), M79 (vs. gap), R80 (≠ K84)
7f5xA Gk domain of drosophila p5cs filament with glutamate (see paper)
33% identity, 55% coverage: 19:232/388 of query aligns to 8:215/236 of 7f5xA
7lnxB I146a mutant of the isopentenyl phosphate kinase from candidatus methanomethylophilus alvus (see paper)
27% identity, 51% coverage: 26:221/388 of query aligns to 7:208/247 of 7lnxB
- binding adenosine-5'-diphosphate: K10 (= K29), S14 (= S33), S174 (= S189), D175 (= D190), I176 (≠ V191), Y180 (= Y195), A182 (= A197), D183 (≠ P198), P184 (= P199), K185 (≠ G200), K204 (≠ E217)
- binding (2E)-3-methylhept-2-en-1-yl dihydrogen phosphate: A50 (≠ S72), G54 (= G76), H55 (≠ R77), A58 (≠ L80), D140 (= D157), V141 (≠ T158), A151 (vs. gap), G154 (≠ N169)
7lnuB Ternary complex of the isopentenyl phosphate kinase from candidatus methanomethylophilus alvus bound to isopentenyl monophosphate and atp (see paper)
27% identity, 55% coverage: 26:240/388 of query aligns to 5:221/261 of 7lnuB
- binding adenosine-5'-diphosphate: K8 (= K29), S12 (= S33), S172 (= S189), D173 (= D190), I174 (≠ V191), Y178 (= Y195), A180 (= A197), K183 (≠ G200), K218 (= K237)
- binding Isopentenyl phosphate: A48 (≠ S72), G52 (= G76), H53 (≠ R77), I149 (vs. gap), S151 (≠ D168), G152 (≠ N169)
7lnuA Ternary complex of the isopentenyl phosphate kinase from candidatus methanomethylophilus alvus bound to isopentenyl monophosphate and atp (see paper)
27% identity, 51% coverage: 26:221/388 of query aligns to 4:200/239 of 7lnuA
- binding adenosine-5'-diphosphate: G9 (= G31), G10 (≠ S32), S11 (= S33), K16 (≠ H38), D172 (= D190), I173 (≠ V191), L176 (= L194), D180 (≠ P198), K182 (≠ G200)
- binding adenosine-5'-triphosphate: K7 (= K29), G9 (= G31), G10 (≠ S32), S11 (= S33), K16 (≠ H38), D172 (= D190), L176 (= L194), A179 (= A197), D180 (≠ P198), K182 (≠ G200)
- binding Isopentenyl phosphate: G46 (= G71), A47 (≠ S72), G51 (= G76), H52 (≠ R77), G136 (≠ N156), I148 (vs. gap), S150 (≠ D168), G151 (≠ N169)
- binding 3-methylbut-3-enyl trihydrogen diphosphate: A47 (≠ S72), G51 (= G76), H52 (≠ R77), G136 (≠ N156), I148 (vs. gap), G151 (≠ N169)
7lntB Ternary complex of the isopentenyl phosphate kinase from candidatus methanomethylophilus alvus bound to benzyl monophosphate and atp (see paper)
27% identity, 55% coverage: 26:240/388 of query aligns to 4:220/260 of 7lntB
- binding adenosine-5'-diphosphate: G9 (= G31), G10 (≠ S32), S11 (= S33), D172 (= D190), I173 (≠ V191), Y177 (= Y195), D180 (≠ P198)
- binding (phenylmethyl) dihydrogen phosphate: A47 (≠ S72), G51 (= G76), H52 (≠ R77), I148 (vs. gap), S150 (≠ D168)
7lntA Ternary complex of the isopentenyl phosphate kinase from candidatus methanomethylophilus alvus bound to benzyl monophosphate and atp (see paper)
27% identity, 51% coverage: 26:221/388 of query aligns to 4:200/238 of 7lntA
- binding adenosine-5'-diphosphate: G9 (= G31), S11 (= S33), K16 (≠ H38), S171 (= S189), D172 (= D190), I173 (≠ V191), Y177 (= Y195), A179 (= A197), D180 (≠ P198), P181 (= P199), K182 (≠ G200), K196 (≠ E217)
- binding (phenylmethyl) dihydrogen phosphate: G46 (= G71), A47 (≠ S72), G51 (= G76), H52 (≠ R77), I148 (vs. gap), S150 (≠ D168), G151 (≠ N169)
7n9dA I74a mutant of the isopentenyl phosphate kinase from candidatus methanomethylophilus alvus (see paper)
26% identity, 55% coverage: 26:240/388 of query aligns to 3:217/253 of 7n9dA
- binding (2E)-3-phenylbut-2-en-1-yl dihydrogen phosphate: G45 (= G71), A46 (≠ S72), G50 (= G76), H51 (≠ R77), A75 (≠ Q100), T79 (≠ A104), G135 (≠ N156), D136 (= D157), V137 (≠ T158), I147 (vs. gap), S149 (≠ D168), G150 (≠ N169), T208 (≠ S227)
- binding adenosine-5'-diphosphate: G8 (= G31), G9 (≠ S32), S10 (= S33), K15 (≠ H38), S170 (= S189), D171 (= D190), I172 (≠ V191), Y176 (= Y195), A178 (= A197), D179 (≠ P198), D201 (≠ A220), G210 (= G233), V211 (≠ M234), K214 (= K237)
3wwmA Crystal structure of lysz from thermus thermophilus with adp (see paper)
27% identity, 62% coverage: 26:264/388 of query aligns to 2:259/269 of 3wwmA
- active site: K5 (= K29), G8 (≠ S32), S38 (= S72), D172 (= D170), K231 (= K237)
- binding adenosine-5'-diphosphate: G8 (≠ S32), G36 (≠ S70), G37 (= G71), S38 (= S72), Y78 (vs. gap), Y200 (≠ P198), M228 (= M234)
O50147 [LysW]-aminoadipate kinase; EC 2.7.2.17 from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see paper)
27% identity, 62% coverage: 26:264/388 of query aligns to 2:259/269 of O50147
- H57 (vs. gap) mutation to A: Shows reduced activity.
- R111 (vs. gap) mutation to E: Shows reduced activity.
- R112 (≠ E109) mutation to E: Shows reduced activity.
- K113 (≠ V110) mutation to E: Loss of activity.
- K117 (≠ R114) mutation to E: Does not bind LysW.
- K123 (≠ Q120) mutation to E: Does not bind LysW.
- K125 (≠ L122) mutation to E: Does not bind LysW.
- R128 (≠ F125) mutation to E: Does not bind LysW.
- R227 (≠ G233) mutation to E: Still binds LysW, but shows reduced activity.
- R230 (≠ T236) mutation to E: Still binds LysW, but shows reduced activity.
- K231 (= K237) mutation to E: Still binds LysW, but shows reduced activity.
7lnxA I146a mutant of the isopentenyl phosphate kinase from candidatus methanomethylophilus alvus (see paper)
27% identity, 48% coverage: 26:213/388 of query aligns to 2:193/234 of 7lnxA
- binding adenosine-5'-diphosphate: K5 (= K29), S9 (= S33), I171 (≠ V191), L174 (= L194), Y175 (= Y195), A177 (= A197), D178 (≠ P198), P179 (= P199), K180 (≠ G200)
- binding (2Z)-3-methylhept-2-en-1-yl trihydrogen diphosphate: G8 (≠ S32), K14 (≠ H38), G44 (= G71), A45 (≠ S72), G46 (≠ V73), G49 (= G76), H50 (≠ R77), G134 (≠ N156), D135 (= D157), A146 (vs. gap), S148 (≠ D168), G149 (≠ N169)
7lnwA I146a mutant of the isopentenyl phosphate kinase from candidatus methanomethylophilus alvus (see paper)
27% identity, 48% coverage: 26:213/388 of query aligns to 3:194/256 of 7lnwA
- binding adenosine-5'-diphosphate: K6 (= K29), S10 (= S33), K15 (≠ H38), D171 (= D190), I172 (≠ V191), Y176 (= Y195), D179 (≠ P198), P180 (= P199), K181 (≠ G200)
- binding [(~{Z})-5-[(azanylidene-$l^{4}-azanylidene)amino]-3-methyl-pent-2-enyl] dihydrogen phosphate: G45 (= G71), A46 (≠ S72), G50 (= G76), H51 (≠ R77), G135 (≠ N156), D136 (= D157), V148 (vs. gap), S149 (≠ D168)
Sites not aligning to the query:
Query Sequence
>WP_020177398.1 NCBI__GCF_000385335.1:WP_020177398.1
MNARAVNKQQTAKPAAVPKLDQFRRITIKVGSSLLVDHEKGCVRRDWLAALADDIASLHG
EGRDILIVSSGSVALGRTVLGLPKGALRLEDSQAAAAVGQIALARLWAEVLGDRMVTAGQ
ILVTFGDTEERQRYLNARETIGRLLDLRAIPVINENDTVATAEIRYGDNDRLAARVATMA
SADLLILLSDVAGLYTAPPGTDPEAKLIPVVPRVTAEIEAMAGGAASAFSRGGMRTKLEA
AKIATTGGTHMLIADGRVAHPVQRIREAALCTWFLTPSNPVTARKKWIGGSLEPRGTVHV
DDGAVKAIFGGASLLPAGVKRVEGNFARGDCVVIRDGQGAEIGRGLVAYDAIEAAQIAGR
NSREIEKVLGIPGRAEIIHRDDMVVGGE
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory