SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_020308877.1 NCBI__GCF_000013785.1:WP_020308877.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q5F8J4 Homoserine dehydrogenase; HDH; HSD; NAD(+)-dependent homoserine dehydrogenase; NAD(+)-dependent HSD; NgHSD; EC 1.1.1.3 from Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) (see paper)
58% identity, 100% coverage: 1:434/434 of query aligns to 1:434/435 of Q5F8J4

query
sites
Q5F8J4

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L45 (≠ T44) mutation to R: Shows a marked increase in the catalytic efficiency with NADP(+).
LS 45:46 (≠ TR 44:45) mutation to RD: Does not impair the catalytic activity with NAD(+). Slightly increases the activity, but slightly decreases the affinity for NADP(+).; mutation to RR: Causes a shift in coenzyme preference from NAD(+) to NADP(+) by a factor of 974. Shows a slight decrease in the catalytic efficiency with NAD(+) and a 4.5-fold increase in catalytic efficiency with NADP(+).

6dzsA Mycobacterial homoserine dehydrogenase thra in complex with NADP
40% identity, 98% coverage: 2:428/434 of query aligns to 3:428/431 of 6dzsA

query
sites
6dzsA

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binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G10), L12 (= L11), G13 (= G12), N14 (≠ T13), V15 (= V14), V45 (≠ T44), R46 (= R45), R47 (≠ H46), R52 (≠ C51), I63 (= I62), L78 (= L77), M79 (≠ I78), P84 (≠ V83), A102 (= A101), K104 (= K103), G306 (= G300), T310 (= T304)

4pg7A Crystal structure of s. Aureus homoserine dehydrogenase at ph7.5 (see paper)
38% identity, 77% coverage: 1:335/434 of query aligns to 2:323/402 of 4pg7A

query
sites
4pg7A

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active site: D190 (= D199), K194 (= K203)
binding lysine: R215 (≠ E224), G219 (≠ R228)

Sites not aligning to the query:

binding lysine: 394, 395

6a0tB Homoserine dehydrogenase k99a mutant from thermus thermophilus hb8 complexed with hse and NADP+ (see paper)
40% identity, 68% coverage: 1:293/434 of query aligns to 1:283/332 of 6a0tB

query
sites
6a0tB

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G

I

V

A
x
L

T
x
V

R
|
R

H
x
D

P

P

N

R

S
x
K

K

P

C
x
R

D

A

T

I

G

P

S

Q

T

E

P

L

I

L

T

R

A

A

D

E

I

P

F

F

D

D

L

L

V

L

N

-

P

-

E

E

I

A

D

D

I

L

V

V

E

E

L
x
A

I
x
M

G
|
G

G

G

Y

V

T

E

V

A

A

P

K

L

E

R

L

L

V

V

L

L

K

P

A

A

I

L

E

E

N

A

G

G

K

I

H

P

V

L

V

I

T

T

A
|
A

N
|
N

K

K

A

A

L

L

I

L

A

A

V

-

H

E

G

A

N

W

E

E

I

S

F

L

A

R

K

P

A

F

R

A

E

E

K

E

G

G

V

L

I

I

V

Y

A

-

F

H

E

E

A

A

S

S

V

V

A

M

G

A

G

G

I

T

P

P

V

A

I

L

K

S

A

F

I

L

R

-

E

E

G

T

L

L

A

R

G

G

N

S

R

E

I

L

N

L

W

E

L

L

A

H

G

G

I

I

I

L

N
|
N

G
|
G

T
|
T

G

T

N

L

F

Y

I

I

L

L

T

Q

E

E

M

M

R

-

E

E

K

K

G

G

R

R

T

T

F

Y

E

A

D

E

V

A

L

L

K

L

E

E

A

A

Q

Q

E

R

L

L

G
|
G

Y
|
Y

A

A

E
|
E

A

A

D

D

P

P

T

T

F

L

D
|
D

V

V

E

E

G

G

I

I

D
|
D

A

A

H

H

K
|
K

L

L

T

T

I

L

L

L

A

A

S

R

I

L

A

L

F

V

G

D

I

P

P

G

L

F

Q

P

F

F

D

A

K

E

A

V

Y

E

T

A

E

Q

G

G

I

I

A

A

R

R

L

L

T

T

T

P

A

E

D

V

V

L

N

Q

Y

K

A

A

E

E

A

A

L

R

G

G

Y

E

R

R

I

V

K

R

H

L

L

-

G

-

V

V

A

A

R

S

R

L

T

F

E

G

A

E

G

G

I

G

E

R

L

W

R

R

-

A

V

V

H

A

P

P

T

R

L

R

I

L

P

P

A

Q

D

D

R

H

L

P

I

L

A

A

N

R

V

A

N

R

G

G

V

-

M

-

N

N

A

A

V

L

M

W

V

V

N

R

G

A

D

R

A

P

V

L

G

G

S

E

T

A

L

F

active site: D191 (= D199), K195 (= K203)
binding l-homoserine: N150 (= N157), G151 (= G158), T152 (= T159), Y178 (= Y186), E180 (= E188), D186 (= D194), K195 (= K203)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G10), G11 (≠ L11), G12 (= G12), T13 (= T13), V14 (= V14), L42 (≠ A43), V43 (≠ T44), R44 (= R45), D45 (≠ H46), K48 (≠ S49), R50 (≠ C51), A73 (≠ L77), M74 (≠ I78), G75 (= G79), A97 (= A101), N98 (= N102), G177 (= G185), E180 (= E188)

Sites not aligning to the query:

binding nadp nicotinamide-adenine-dinucleotide phosphate: 289, 290, 294

6a0sA Homoserine dehydrogenase from thermus thermophilus hb8 complexed with hse and NADPH (see paper)
40% identity, 68% coverage: 1:293/434 of query aligns to 1:283/331 of 6a0sA

query
sites
6a0sA

M

M

K

E

P

A

V

L

K

K

V

I

G

A

I

L

C

L

G

G

L
x
G

G
|
G

T
|
T

V
|
V

G

G

G

S

G

A

T

F

F

Y

N

N

V

L

L

V

K

L

R

E

N

R

A

A

E

E

I

L

A

S

R

A

R

-

A

F

G

G

R

V

G

V

I

P

E

R

V

F

A

L

Q

G

I

V

A
x
L

T
x
V

R
|
R

H
x
D

P

P

N

R

S
x
K

K

P

C
x
R

D

A

T

I

G

P

S

Q

T

E

P

L

I

L

T

R

A

A

D

E

I

P

F

F

D

D

L

L

V

L

N

-

P

-

E

E

I

A

D

D

I

L

V

V

E

E

L
x
A

I
x
M

G

G

Y

V

T

E

V

A

A

P

K

L

E

R

L

L

V

V

L

L

K

P

A

A

I

L

E

E

N

A

G

G

K

I

H

P

V

L

V

I

T

T

A
|
A

N

N

K
|
K

A

A

L

L

I

L

A

A

V

-

H

E

G

A

N

W

E

E

I

S

F

L

A

R

K

P

A

F

R

A

E

E

K

E

G

G

V

L

I

I

V

Y

A

-

F

H

E

E

A

A

S

S

V

V

A

M

G

A

G

G

I

T

P

P

V

A

I

L

K

S

A

F

I

L

R

-

E

E

G

T

L

L

A

R

G

G

N

S

R

E

I

L

N

L

W

E

L

L

A

H

G

G

I

I

I

L

N
|
N

G
|
G

T
|
T

G

T

N

L

F

Y

I

I

L

L

T

Q

E

E

M

M

R

-

E

E

K

K

G

G

R

R

T

T

F

Y

E

A

D

E

V

A

L

L

K

L

E

E

A

A

Q

Q

E

R

L

L

G
|
G

Y
|
Y

A

A

E
|
E

A

A

D

D

P

P

T

T

F

L

D
|
D

V

V

E

E

G

G

I

I

D
|
D

A

A

H

H

K
|
K

L

L

T

T

I

L

L

L

A

A

S

R

I

L

A

L

F

V

G

D

I

P

P

G

L

F

Q

P

F

F

D

A

K

E

A

V

Y

E

T

A

E

Q

G

G

I

I

A

A

R

R

L

L

T

T

T

P

A

E

D

V

V

L

N

Q

Y

K

A

A

E

E

A

A

L

R

G

G

Y

E

R

R

I

V

K

R

H

L

L

-

G

-

V

V

A

A

R

S

R

L

T

F

E

G

A

E

G

G

I

G

E

R

L

W

R

R

-

A

V

V

H

A

P

P

T

R

L

R

I

L

P

P

A

Q

D

D

R

H

L

P

I

L

A

A

N

R

V

A

N

R

G

G

V

-

M

-

N

N

A

A

V

L

M

W

V

V

N

R

G

A

D

R

A

P

V

L

G

G

S

E

T

A

L

F

active site: D191 (= D199), K195 (= K203)
binding l-homoserine: K99 (= K103), N150 (= N157), G151 (= G158), T152 (= T159), Y178 (= Y186), E180 (= E188), D186 (= D194), K195 (= K203)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (≠ L11), G12 (= G12), T13 (= T13), V14 (= V14), L42 (≠ A43), V43 (≠ T44), R44 (= R45), D45 (≠ H46), K48 (≠ S49), R50 (≠ C51), A73 (≠ L77), M74 (≠ I78), A97 (= A101), K99 (= K103), G177 (= G185), E180 (= E188)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 289, 290, 294

2ejwA Homoserine dehydrogenase from thermus thermophilus hb8
40% identity, 68% coverage: 1:293/434 of query aligns to 1:283/331 of 2ejwA

query
sites
2ejwA

M

M

K

E

P

A

V

L

K

K

V

I

G

A

I

L

C

L

G

G

L

G

G

G

T

T

V

V

G

G

G

S

G

A

T

F

F

Y

N

N

V

L

L

V

K

L

R

E

N

R

A

A

E

E

I

L

A

S

R

A

R

-

A

F

G

G

R

V

G

V

I

P

E

R

V

F

A

L

Q

G

I

V

A

L

T

V

R

R

H

D

P

P

N

R

S

K

K

P

C

R

D

A

T

I

G

P

S

Q

T

E

P

L

I

L

T

R

A

A

D

E

I

P

F

F

D

D

L

L

V

L

N

-

P

-

E

E

I

A

D

D

I

L

V

V

E

E

L

A

I

M

G

G

Y

V

T

E

V

A

A

P

K

L

E

R

L

L

V

V

L

L

K

P

A

A

I

L

E

E

N

A

G

G

K

I

H

P

V

L

V

I

T

T

A

A

N

N

K

K

A

A

L

L

I

L

A

A

V

-

H

E

G

A

N

W

E

E

I

S

F

L

A

R

K

P

A

F

R

A

E

E

K

E

G

G

V

L

I

I

V

Y

A

-

F

H

E
|
E

A

A

S

S

V
|
V

A
x
M

G
x
A

G

G

I
x
T

P

P

V

A

I

L

K

S

A

F

I

L

R

-

E

E

G

T

L

L

A

R

G

G

N

S

R

E

I

L

N

L

W

E

L

L

A

H

G

G

I

I

I

L

N

N

G

G

T

T

G

T

N

L

F

Y

I

I

L

L

T

Q

E

E

M

M

R

-

E

E

K

K

G

G

R

R

T

T

F

Y

E

A

D

E

V

A

L

L

K

L

E

E

A

A

Q

Q

E

R

L

L

G

G

Y

Y

A

A

E

E

A

A

D

D

P

P

T

T

F

L

D

D

V

V

E

E

G

G

I

I

D
|
D

A

A

H

H

K
|
K

L

L

T

T

I

L

L

L

A

A

S

R

I

L

A

L

F

V

G

D

I

P

P

G

L

F

Q

P

F

F

D

A

K

E

A

V

Y

E

T

A

E

Q

G

G

I

I

A

A

R

R

L

L

T

T

T

P

A

E

D

V

V

L

N

Q

Y

K

A

A

E

E

A

A

L

R

G

G

Y

E

R

R

I

V

K

R

H

L

L

-

G

-

V

V

A

A

R

S

R

L

T

F

E

G

A

E

G

G

I

G

E

R

L

W

R

R

-

A

V

V

H

A

P

P

T

R

L

R

I

L

P

P

A

Q

D

D

R

H

L

P

I

L

A

A

N

R

V

A

N

R

G

G

V

-

M

-

N

N

A

A

V

L

M

W

V

V

N

R

G

A

D

R

A

P

V

L

G

G

S

E

T

A

L

F

active site: D191 (= D199), K195 (= K203)
binding magnesium ion: E121 (= E127), V124 (= V130), M125 (≠ A131), A126 (≠ G132), T128 (≠ I134)

F9VNG5 Homoserine dehydrogenase; HDH; HSD; StHSD; EC 1.1.1.3 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 3 papers)
39% identity, 60% coverage: 56:317/434 of query aligns to 50:301/304 of F9VNG5

query
sites
F9VNG5

T

T

P

P

-

D

I

L

T

E

A

G

D

D

I

V

F

F

D

K

L

A

V

F

N

E

N

K

P

I

E

K

I

P

D

D

I

I

V

I

V

V

E

D

L

V

I
x
S

G

S

G

A

Y

N

T

Y

V

N

A

N

K

G

E

E

-

P

-

S

-

L

-

S

L

L

V

Y

L

K

K

E

A

A

I

I

E

K

N

D

G

G

K

V

H

N

V

I

T

T

A
x
T

N

N

K
|
K

A

A

L

P

I

L

A

A

V

L

H

A

G

F

N

N

E

E

I

I

F

F

A

S

K

L

A

A

R

R

E

S

K

K

G

G

V

V

I

K

V

I

A

G

F

F

E

Q

A

G

S

T

V

V

A

M

G

S

G

G

I

T

P

P

V

S

I

I

K

N

A

L

I

Y

R

R

E

V

G

-

L

L

A

P

G

G

N

S

R

R

I

V

N

I

W

K

L

I

A

R

G

G

I

I

I

L

N

N

G

G

T

T

G

T

N

N

F

F

I

I

L

L

T

T

E

L

M

M

R

N

E

-

K

K

G

G

R

V

T

S

F

F

E

E

D

E

V

A

L

L

K

K

E

E

A

A

Q

Q

E

R

L

R

G
|
G

Y

Y

A

A

E
|
E

A

E

D

D

P

P

T

T

F

L

D

D

V

I

E

N

G

G

I

F

D

D

A

A

H

A

K

K

L

I

T

T

I

I

L

L

A

A

S

N

I

F

A

M

F

I

G

G

I

N

P

S

L

V

Q

T

F

I

D

K

K

D

A

V

Y

K

T

F

E

E

G

G

I

I

A

N

R

R

L

-

T

-

A

-

D

D

V

L

N

P

Y

K

A

N

E

E

A

-

L

-

G

-

Y

-

R

K

I

I

K

K

H

L

L

I

G

A

V

Y

A

A

R

D

R

E

T

K

E

-

A

-

G

-

I

-

E

E

L

V

R

W

V

V

H

K

P

P

T

L

L

P

I

I

P

S

A

Q

D

D

R

D

L

P

I

L

A

Y

N

N

V

V

N

D

G

G

V

V

M

E

N

N

A

A

V

L

M

E

V

I

N

T

G

T

D

D

A

-

V

I

G

Q

S

S

T

I

L

L

Y

I

Y

R

G

G

A

P

G

G

A

A

G
|
G

M

P

E

V

P

N

T

A

A

A

S

Y

A

G

V

A

V

L

A

S

D

D

V

L

V

I

D

L

V

L

T

K

R

R

S73 (≠ I78) binding NADP(+)
T100 (≠ A101) binding NADP(+)
K102 (= K103) binding NADP(+)
G182 (= G185) binding NADP(+)
E185 (= E188) binding NADP(+)
G284 (= G300) binding NADP(+)

Sites not aligning to the query:

8 binding NADP(+)
10 binding NADP(+)
11 binding NADP(+)
38 binding NADP(+)
39 binding NADP(+)
303 modified: Interchain

7f4cA The crystal structure of the immature holo-enzyme of homoserine dehydrogenase complexed with NADP and 1,4-butandiol from the hyperthermophilic archaeon sulfurisphaera tokodaii. (see paper)
38% identity, 60% coverage: 56:317/434 of query aligns to 50:297/300 of 7f4cA

query
sites
7f4cA

T

T

P

P

-

D

I

L

T

E

A

G

D

D

I

V

F

F

D

K

L

A

V

F

N

E

N

K

P

I

E

K

I

P

D

D

I

I

V

I

V

V

E

D

L
x
V

I
x
S

G
x
S

G

A

Y

N

T

Y

V

N

A

N

K

G

E

E

-

P

-

S

-

L

-

S

L

L

V

Y

L

K

K

E

A

A

I

I

E

K

N

D

G

G

K

V

H

N

V

I

T

T

A
x
T

N

N

K
|
K

A

A

L

P

I

L

A

A

V

L

H

A

G

F

N

N

E

E

I

I

F

F

A

S

K

L

A

A

R

R

E

S

K

K

G

G

V

V

I

K

V

I

A

G

F

F

E

Q

A
x
G

S

T

V

V

A

M

G
x
S

G

G

I

T

P

P

V

S

I

I

K

N

A

L

I

Y

R

R

E

V

G

-

L

L

A

P

G

G

N

S

R

R

I

V

N

I

W

K

L

I

A

R

G

G

I

I

I

L

N

N

G

G

T

T

G

T

N

N

F

F

I

I

L

L

T

T

E

L

M

M

R

-

E

N

K

K

G

G

R

V

T

S

F

F

E

E

D

E

V

A

L

L

K

K

E

E

A

A

Q

Q

E

R

L

R

G

G

Y

Y

A

A

E
|
E

A

E

D

D

P

P

T

T

F

L

D

D

V

I

E

N

G

G

I

F

D

D

A

A

H

A

K

K

L

I

T

T

I

I

L

L

A

A

S

N

I

F

A

M

F

I

G

G

I

N

P

S

L

V

Q

T

F

I

D

K

K

D

A

V

Y

K

T

F

E

E

G

G

I

I

A

N

R

R

L

-

T

-

A

-

D

-

V

-

N

-

Y

-

A

-

E

-

A

-

L

-

G

D

Y

L

R

K

I

I

K

K

H

L

L

I

G

A

V

Y

A

A

R

D

R

E

T

K

E

-

A

-

G

-

I

-

E

E

L

V

R

W

V

V

H

K

P

P

T

L

L

P

I

I

P

S

A

Q

D

D

R

D

L

P

I

L

A

Y

N

N

V

V

N

D

G

G

V

V

M

E

N

N

A

A

V

L

M

E

V

I

N

T

G

T

D

D

A

-

V

I

G

Q

S

S

T

I

L

L

Y

I

Y

R

G

G

A

P

G

G

A

A

G
|
G

M

P

E

V

P

N

T
x
A

A

A

S

Y

A

G

V

A

V

L

A

S

D

D

V

L

V

I

D

L

V

L

T

K

R

R

binding nadp nicotinamide-adenine-dinucleotide phosphate: V72 (≠ L77), S73 (≠ I78), S74 (≠ G79), T100 (≠ A101), K102 (= K103), G127 (≠ A128), S131 (≠ G132), E185 (= E188), G280 (= G300), A284 (≠ T304)

Sites not aligning to the query:

binding nadp nicotinamide-adenine-dinucleotide phosphate: 6, 7, 8, 9, 10, 11, 37, 38, 39

5x9dA Crystal structure of homoserine dehydrogenase in complex with l- cysteine and NAD (see paper)
38% identity, 60% coverage: 56:317/434 of query aligns to 50:299/302 of 5x9dA

query
sites
5x9dA

T

T

P

P

-

D

I

L

T

E

A

G

D

D

I

V

F

F

D

K

L

A

V

F

N

E

N

K

P

I

E

K

I

P

D

D

I

I

V

I

V

V

E

D

L
x
V

I
x
S

G
x
S

G

A

Y

N

T

Y

V

N

A

N

K

G

E

E

-
x
P

-

S

-

L

-

S

L

L

V

Y

L

K

K

E

A

A

I

I

E

K

N

D

G

G

K

V

H

N

V

I

T

T

A
x
T

N
|
N

K
|
K

A

A

L

P

I

L

A

A

V

L

H

A

G

F

N

N

E

E

I

I

F

F

A

S

K

L

A

A

R

R

E

S

K

K

G

G

V

V

I

K

V

I

A

G

F

F

E

Q

A
x
G

S

T

V

V

A

M

G
x
S

G

G

I

T

P

P

V

S

I

I

K

N

A

L

I

Y

R

R

E

V

G

-

L

L

A

P

G

G

N

S

R

R

I

V

N

I

W

K

L

I

A

R

G

G

I

I

I

L

N
|
N

G
|
G

T
|
T

G

T

N

N

F

F

I

I

L

L

T

T

E

L

M

M

R

N

E

-

K

K

G

G

R

V

T

S

F

F

E

E

D

E

V

A

L

L

K

K

E

E

A

A

Q

Q

E

R

L

R

G

G

Y
|
Y

A
|
A

E
|
E

A

E

D

D

P

P

T

T

F

L

D
|
D

V

I

E

N

G

G

I

F

D
|
D

A

A

H

A

K
|
K

L

I

T

T

I

I

L

L

A

A

S

N

I

F

A

M

F

I

G

G

I

N

P

S

L

V

Q

T

F

I

D

K

K

D

A

V

Y

K

T

F

E

E

G

G

I

I

A

N

R

R

L

-

T

-

A

-

D

-

V

-

N

-

Y

-

A

-

E

D

A

P

L

K

G

N

Y

E

R

K

I

I

K

K

H

L

L

I

G

A

V

Y

A

A

R

D

R

E

T

K

E

-

A

-

G

-

I

-

E

E

L

V

R

W

V

V

H

K

P

P

T

L

L

P

I

I

P

S

A

Q

D

D

R

P

L

L

I

Y

A

-

N

N

V

V

N

D

G

G

V

V

M

E

N

N

A

A

V

L

M

E

V

I

N

T

G

T

D

D

A

-

V

I

G

Q

S

S

T

I

L

L

Y

I

Y

R

G

G

A

P

G

G

A
|
A

G
|
G

M

P

E

V

P

N

T
x
A

A

A

S

Y

A

G

V

A

V

L

A

S

D

D

V

L

V

I

D

L

V

L

T

K

R

R

active site: D196 (= D199), K200 (= K203)
binding (2R)-3-[[(4S)-3-aminocarbonyl-1-[(2R,3R,4S,5R)-5-[[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxymethyl]-3,4-bis(oxidanyl)oxolan-2-yl]-4H-pyridin-4-yl]sulfanyl]-2-azanyl-propanoic acid: V72 (≠ L77), S73 (≠ I78), S74 (≠ G79), P82 (vs. gap), T100 (≠ A101), N101 (= N102), K102 (= K103), G127 (≠ A128), S131 (≠ G132), N155 (= N157), G156 (= G158), T157 (= T159), Y183 (= Y186), A184 (= A187), E185 (= E188), D191 (= D194), D196 (= D199), K200 (= K203), A281 (= A299), G282 (= G300), A286 (≠ T304)

Sites not aligning to the query:

binding (2R)-3-[[(4S)-3-aminocarbonyl-1-[(2R,3R,4S,5R)-5-[[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxymethyl]-3,4-bis(oxidanyl)oxolan-2-yl]-4H-pyridin-4-yl]sulfanyl]-2-azanyl-propanoic acid: 6, 7, 8, 9, 10, 11, 37, 38

4xb1A Hyperthermophilic archaeal homoserine dehydrogenase in complex with NADPH (see paper)
31% identity, 61% coverage: 64:326/434 of query aligns to 78:319/319 of 4xb1A

query
sites
4xb1A

D

E

L

L

V

V

N

E

P

V

E

K

I

P

D

N

I

I

V

L

V

V

E

D

L

-

I
x
V

G
x
S

Y

W

T

D

V

E

A

A

K

H

E

E

L

M

V

Y

L

K

K

V

A

A

I

L

E

G

N

E

G

G

K

I

H

S

V

V

T

T

A
x
S

N

N

K
|
K

A

P

L

P

I

I

A

A

V

N

H

Y

G

Y

N

D

E

E

I

L

F

M

A

N

K

L

A

A

R

K

E

E

K

N

G

N

V

A

I

G

V

I

A

F

F

F

E

E

A
x
S

S

T

V

V

A

M

G

A

G

G

I

T

P

P

V

I

I

I

K

G

A

V

I

L

R

R

E

E

G

N

L

L

A

L

G

G

N

E

R

N

I

I

N

K

W

R

L

I

A

D

G

A

I

V

N

N

G

A

T

S

G

T

N

T

F

F

I

I

L

L

T

T

E

K

M

M

R

S

E

E

K

-

G

G

R

K

T

T

F

L

E

D

D

D

V

A

L

I

K

E

E

E

A

A

Q

K

E

S

L

L

G

G

Y

I

A

L

E

E

A

E

D

D

P

P

T

S

F

K

D

D

V

I

E

D

G

G

I

I

D
|
D

A

A

A

Y

H

Y

K
|
K

L

A

T

K

I

I

L

L

A

H

S

W

I

V

A

S

F

Y

G

G

I

E

P

P

L

P

Q

E

F

E

D

E

K

E

A

-

Y

-

T

-

E

-

G

-

I

-

A

-

R

R

L

L

T

G

T

I

A

R

D

E

V

V

N

R

Y

D

A

A

E

-

A

-

L

-

G

-

Y

-

R

-

I

-

K

R

H

N

L

V

G

R

V

L

A

V

R

A

R

Q

T

V

E

S

A

K

G

G

I

-

E

K

L

I

R

S

V

V

H

K

P

P

T

R

L

K

I

L

P

S

A

S

D

D

R

N

L

P

I

L

A

L

N

-

V

V

N

E

G

G

V

V

M

Q

N

N

A

A

V

A

M

V

V

I

N

R

G

T

D

N

A

N

V

L

G

G

S

E

T

V

L

I

Y

L

Y

K

G

G

A

P

G

G

A
x
G

G

G

M

G

E

R

P

V

T
|
T

A

A

S

S

A

G

V

V

V

F

A

T

D

D

V

I

D

K

V

A

T

T

R

L

A

K

L

F

T

-

D

-

P

P

N

N

N

L

R

R

active site: D211 (= D199), K215 (= K203)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: V91 (≠ I78), S92 (≠ G79), S93 (≠ G80), S114 (≠ A101), K116 (= K103), S141 (≠ A128), G295 (≠ A299), T300 (= T304)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 8, 10, 11, 12, 13, 39, 40, 57

O58802 Homoserine dehydrogenase; HDH; HSD; HseDH; EC 1.1.1.3 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see paper)
31% identity, 61% coverage: 64:326/434 of query aligns to 78:319/319 of O58802

query
sites
O58802

D

E

L

L

V

V

N

E

P

V

E

K

I

P

D

N

I

I

V

L

V

V

E

D

L

-

I

V

G
x
S

Y

W

T

D

V

E

A

A

K

H

E

E

L

M

V

Y

L

K

K

V

A

A

I

L

E

G

N

E

G

G

K

I

H

S

V

V

T

T

A
x
S

N

N

K
|
K

A

P

L

P

I

I

A

A

V

N

H

Y

G

Y

N

D

E

E

I

L

F

M

A

N

K

L

A

A

R

K

E

E

K

N

G

N

V

A

I

G

V

I

A

F

F

F

E
|
E

A

S

S

T

V
|
V

A

M

G
x
A

G

G

I
x
T

P

P

V

I

I

I

K

G

A

V

I

L

R

R

E

E

G

N

L

L

A

L

G

G

N

E

R

N

I

I

N

K

W

R

L

I

A

D

G

A

I

V

N

N

G

A

T

S

G

T

N

T

F

F

I

I

L

L

T

T

E

K

M

M

R

S

E

E

K

-

G

G

R

K

T

T

F

L

E

D

D

D

V

A

L

I

K

E

E

E

A

A

Q

K

E

S

L

L

G

G

Y

I

A

L

E

E

A

E

D

D

P

P

T

S

F

K

D

D

V

I

E

D

G

G

I

I

D

D

A

A

A

Y

H

Y

K

K

L

A

T

K

I

I

L

L

A

H

S

W

I

V

A

S

F

Y

G

G

I

E

P

P

L

P

Q

E

F

E

D

E

K

E

A

-

Y

-

T

-

E

-

G

-

I

-

A

-

R

R

L

L

T

G

T

I

A

R

D

E

V

V

N

R

Y

D

A

A

E

-

A

-

L

-

G

-

Y

-

R

-

I

-

K

R

H

N

L

V

G

R

V

L

A

V

R

A

R

Q

T

V

E

S

A

K

G

G

I

-

E

K

L

I

R

S

V

V

H

K

P

P

T

R

L

K

I

L

P

S

A

S

D

D

R

N

L

P

I

L

A

L

N

-

V

V

N

E

G

G

V

V

M

Q

N

N

A

A

V

A

M

V

V

I

N

R

G

T

D

N

A

N

V

L

G

G

S

E

T

V

L

I

Y

L

Y

K

G

G

A

P

G

G

A

G

G
|
G

M

G

E

R

P

V

T

T

A

A

S

S

A

G

V

V

V

F

A

T

D

D

V

I

D

K

V

A

T

T

R

L

A

K

L

F

T

-

D

-

P

P

N

N

N

L

R

R

S92 (≠ G79) binding NADPH
S93 (≠ G80) binding NADPH
S114 (≠ A101) binding NADPH
K116 (= K103) binding NADPH
E140 (= E127) binding Na(+)
V143 (= V130) binding Na(+)
A145 (≠ G132) binding Na(+)
T147 (≠ I134) binding Na(+)
G296 (= G300) binding NADPH

Sites not aligning to the query:

10 binding NADPH
12 binding NADPH
13 binding NADPH
40 binding NADPH; R→A: Increases activity with NADP.
57 binding NADPH; K→A: Increases activity with NADP.

4xb2A Hyperthermophilic archaeal homoserine dehydrogenase mutant in complex with NADPH (see paper)
31% identity, 61% coverage: 64:326/434 of query aligns to 78:319/319 of 4xb2A

query
sites
4xb2A

D

E

L

L

V

V

N

E

P

V

E

K

I

P

D

N

I

I

V

L

V

V

E

D

L

-

I
x
V

G
x
S

Y

W

T

D

V

E

A

A

K

H

E

E

L

M

V

Y

L

K

K

V

A

A

I

L

E

G

N

E

G

G

K

I

H

S

V

V

T

T

A
x
S

N
|
N

K
|
K

A

P

L

P

I

I

A

A

V

N

H

Y

G

Y

N

D

E

E

I

L

F

M

A

N

K

L

A

A

R

K

E

E

K

N

G

N

V

A

I

G

V

I

A

F

F

F

E

E

A
x
S

S

T

V

V

A

M

G

A

G

G

I

T

P

P

V

I

I

I

K

G

A

V

I

L

R

R

E

E

G

N

L

L

A

L

G

G

N

E

R

N

I

I

N

K

W

R

L

I

A

D

G

A

I

V

N

N

G
x
A

T
x
S

G

T

N

T

F

F

I

I

L

L

T

T

E

K

M

M

R

S

E

E

K

-

G

G

R

K

T

T

F

L

E

D

D

D

V

A

L

I

K

E

E

E

A

A

Q

K

E

S

L

L

G

G

Y

I

A

L

E

E

A

E

D

D

P

P

T

S

F

K

D
|
D

V

I

E

D

G

G

I

I

D
|
D

A

A

A

Y

H

Y

K
|
K

L

A

T

K

I

I

L

L

A

H

S

W

I

V

A

S

F

Y

G

G

I

E

P

P

L

P

Q

E

F

E

D

E

K

E

A

-

Y

-

T

-

E

-

G

-

I

-

A

-

R

R

L

L

T

G

T

I

A

R

D

E

V

V

N

R

Y

D

A

A

E

-

A

-

L

-

G

-

Y

-

R

-

I

-

K

R

H

N

L

V

G

R

V

L

A

V

R

A

R

Q

T

V

E

S

A

K

G

G

I

-

E

K

L

I

R

S

V

V

H

K

P

P

T

R

L

K

I

L

P

S

A

S

D

D

R

N

L

P

I

L

A

L

N

-

V

V

N

E

G

G

V

V

M

Q

N

N

A

A

V

A

M

V

V

I

N

R

G

T

D

N

A

N

V

L

G

G

S

E

T

V

L

I

Y

L

Y

K

G

G

A

P

G

G

A
x
G

G

G

M

G

E

R

P

V

T
|
T

A

A

S

S

A

G

V

V

V

F

A

T

D

D

V

I

D

K

V

A

T

T

R

L

A

K

L

F

T

-

D

-

P

P

N

N

N

L

R

R

active site: D211 (= D199), K215 (= K203)
binding l-homoserine: A171 (≠ G158), S172 (≠ T159), D206 (= D194), K215 (= K203)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: V91 (≠ I78), S92 (≠ G79), S93 (≠ G80), S114 (≠ A101), N115 (= N102), K116 (= K103), S141 (≠ A128), G295 (≠ A299), T300 (= T304)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 8, 10, 11, 12, 13, 40

3jsaA Homoserine dehydrogenase from thermoplasma volcanium complexed with NAD
32% identity, 52% coverage: 1:226/434 of query aligns to 1:239/321 of 3jsaA

query
sites
3jsaA

M

M

K

K

P

T

V

I

K

N

V

L

G

S

I

I

C

F

G
|
G

L
|
L

G
|
G

T
x
N

V
|
V

G

G

G

L

G

N

T

L

F

L

N

R

V

I

L

I

K

R

R

S

N

F

A

N

E

E

I

-

A

-

R

N

R

R

A

L

G

G

R

L

G

K

I

F

E

N

V

V

A

V

Q

F

I

V

A

A

T
x
D

R
x
S

-

L

-

H

-

S

H

Y

P

Y

N

N

S

E

K

R

C

I

D

D

T

I

G

G

S

K

T

V

-

I

-

S

-

Y

-

K

-

E

-

K

-

G

P

S

I

L

T

D

A

S

D

L

I

E

F

Y

D

E

L

S

V

I

N

S

N

A

P

S

E

E

-

A

-

L

-

A

-

R

-

D

I

F

D

D

I

I

V

V

E

D

L
x
A

I
x
T

-
x
P

G

A

G

S

Y

A

T

D

V

G

A

K

K

K

E

E

L

L

V

A

L

F

-

Y

-

K

K

E

A

T

I

F

E

E

N

N

G

G

K

K

H

D

V

V

T

T

A
|
A

N
|
N

K

K

A

S

L

G

I

L

A

A

V

N

H

F

G

W

N

P

E

E

I

I

F

M

A

E

K

Y

A

A

R

R

E

S

K

N

G

N

V

R

I

R

V

I

A

R

F

Y

E

E

A
|
A

S

T

V

V

A

A

G

G

I

V

P

P

V

L

I

F

K

S

A

F

I

I

R

D

E

Y

G

S

L

V

A

L

G

P

N

S

R

R

I

I

N

K

W

K

L

F

A

R

G

G

I

I

I

V

N

S

G

L

T

T

G

I

N

N

F

Y

I

F

L

I

T

R

E

E

M

L

R

A

E

N

K

K

G

-

R

R

T

E

F

F

E

D

D

D

V

V

L

L

K

S

E

E

A

A

Q

T

E

K

L

L

G

G

Y

I

A

V

E

N

A

Y

D

K

P

D

T

-

F

-

D

D

V

L

E

T

G

G

I

L

D
|
D

A

A

H

R

K
|
K

L

S

T

V

I

I

L

L

A

C

S

N

I

H

A

L

F

Y

G

G

I

S

P

S

L

Y

Q

R

F

L

D

S

K

D

A

V

Y

F

T

Y

E

E

G

G

I

I

active site: D212 (= D199), K216 (= K203)
binding nicotinamide-adenine-dinucleotide: G10 (= G10), L11 (= L11), G12 (= G12), N13 (≠ T13), V14 (= V14), D42 (≠ T44), S43 (≠ R45), A90 (≠ L77), T91 (≠ I78), P92 (vs. gap), A117 (= A101), N118 (= N102), A144 (= A128)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 308

3ingA Crystal structure of homoserine dehydrogenase (np_394635.1) from thermoplasma acidophilum at 1.95 a resolution
32% identity, 32% coverage: 86:226/434 of query aligns to 97:236/319 of 3ingA

query
sites
3ingA

E

S

L

L

V

Y

L

R

K

M

A

A

I

F

E

E

N

S

G

G

K

M

H

N

V

V

T

T

A
|
A

N
|
N

K
|
K

A

S

L

G

I

L

A

A

V

N

H

K

G

W

N

H

E

D

I

I

F

M

A

D

K

S

A

A

R

N

E

Q

K

N

G

S

V

K

I

Y

V

I

A

R

F

Y

E

E

A
|
A

S

T

V

V

A

A

G

G

I

V

P

P

V

L

I

F

K

S

A

V

I

L

R

D

E

Y

G

S

L

I

A

L

G

P

N

S

R

K

I

V

N

K

W

R

L

F

A

R

G

G

I

I

I

V

N

S

G

S

T

T

G

I

N

N

F

Y

I

V

L

I

T

R

E

N

M

M

R

-

E

A

K

N

G

G

R

R

T

S

F

L

E

R

D

D

V

V

L

V

K

D

E

D

A

A

Q

I

E

K

L

K

G

G

Y

I

A

A

E
|
E

A
x
S

D

N

P

P

T

Q

F

D

D

D

V

L

E

N

G

G

I

L

D
|
D

A

A

H

R

K
|
K

L

S

T

V

I

I

L

L

A

V

S

N

I

H

A

I

F

F

G

G

I

T

P

E

L

Y

Q

T

F

L

D

N

K

D

A

V

Y

E

T

Y

E

S

G

G

I

V

active site: D209 (= D199), K213 (= K203)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: A112 (= A101), N113 (= N102), K114 (= K103), A139 (= A128), E198 (= E188), S199 (≠ A189)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 9, 10, 11, 12, 13, 38, 39, 57, 85, 86, 87, 302, 305

O94671 Homoserine dehydrogenase; HDH; HSD; EC 1.1.1.3 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
28% identity, 48% coverage: 4:211/434 of query aligns to 8:239/376 of O94671

query
sites
O94671

V

V

K

N

V

V

G

A

I

I

C

V

G

G

L

T

G

G

T

N

V

I

G

G

G

E

T

L

F

L

N

N

V

Q

L

I

K

K

R

G

N

F

A

N

E

E

E

N

I

A

A

S

R

T

R

N

A

G

G

T

R

T

G

S

I

F

E

N

V

V

A

V

Q

A

I

I

A

S

T

S

R

M

-

E

-

G

H

H

P

Y

N

V

S

S

K

K

-

D

-

Y

-

Q

-

P

-

I

-

N

-

L

-

S

-

E

-

W

-

K

-

N

-

L

-

T

-

S

C

Q

D

N

T

Q

G

G

S

A

T

Y

P

S

I

L

T

D

A

A

D

L

I

V

F

D

D

F

L

L

V

A

N

K

N

S

P

P

E

L

I

P

D

A

I

I

V

L

V

V

E

D

L

N

I

T

G

A

G

S

Y

E

T

A

V

I

A

A

K

Q

E

S

L

Y

V

P

L

-

K

K

A

F

I

L

E

S

N

K

G

K

K

I

H

N

V

I

V

A

T

T

A

P

N

N

K

K

A

K

L

A

I

F

A

S

V

A

H

S

G

N

N

D

-

V

-

Y

-

Q

E

N

I

I

F

I

A

K

K

A

A

S

R

K

E

E

K

S

G

G

V

A

I

L

V

L

A

M

F

H

E

E

A

A

S

S

V

V

A

G

G

A

G

G

I

L

P

P

V

I

I

I

K

S

A

T

I

L

R

K

E

E

G

L

L

I

A

A

-

T

G

G

N

D

R

E

I

I

N

I

W

K

L

I

A

E

G

G

I

I

I

F

N

S

G

G

T

T

G

L

N

S

F

Y

I

I

L

F

T

N

E

V

M

W

R

S

E

P

K

N

G

G

R

K

-

K

-

G

-

T

-

A

T
x
S

F

F

E

S

D

D

V

I

L

V

K

K

E

I

A

A

Q

K

E

Q

L

N

G

G

Y

Y

A

T

E

E

A

P

D

D

P

P

T

R

F

D

D

D

V

L

E

N

G

G

I

M

D

D

A

V

A

A

H

R

K

K

L

V

T

T

I

I

L

L

A

S

S

R

I

I

A

A

S201 (≠ T173) modified: Phosphoserine

P31116 Homoserine dehydrogenase; HDH; HSD; EC 1.1.1.3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
31% identity, 37% coverage: 53:211/434 of query aligns to 68:231/359 of P31116

query
sites
P31116

T

T

G

K

S

T

T

L

P

P

I

L

T

D

A

D

D

L

I

I

F

A

D
x
H

L

L

V

K

N

T

N

S

P

P

E

K

I

P

D

V

I

I

V

L

V

V

E

D

L

N

I
x
T

G

S

Y

A

T

Y

V

I

A

A

K

G

E

-

L

F

V

Y

L

T

K

K

A

F

I

V

E

E

N

N

G

G

K

I

H

S

V

I

V

A

T

T

A

P

N

N

K

K

A

K

L

A

I

F

A

S

-

S

-

D

-

L

V

A

H

T

G

W

N

K

E

A

I

L

F

F

A

S

K

N

A

K

R

P

E

T

K

N

G

G

V

-

I

F

V

V

A

Y

F

H

E
|
E

A

A

S

T

V
|
V

A

G

G
x
A

G

G

I
x
L

P

P

V

I

I

I

K

S

A

F

I

L

R

R

E

E

G

I

L

I

-

Q

A

T

G

G

N

D

R

E

I

V

N

E

W

K

L

I

A

E

G

G

I

I

I

F

N

S

G

G

T

T

G

L

N

S

F

Y

I

I

L

F

T

N

E

E

M

F

-

S

-

T

-

S

R

Q

E

A

K

N

G

D

R

V

T

K

F

F

E

S

D

D

V

V

L

V

K

K

E

V

A

A

Q

K

E

K

L

L

G

G

Y

Y

A

T

E
|
E

A

P

D

D

P

P

T

R

F

D

D

D

V

L

E

N

G

G

I

L

D
|
D

A

V

A

A

H

R

K
|
K

L

V

T

T

I

I

L

V

A

G

S

R

I

I

A

S

H79 (≠ D64) mutation to A: Reduces kcat 2-fold.
T93 (≠ I78) binding NAD(+)
E143 (= E127) binding Na(+)
V146 (= V130) binding Na(+)
A148 (≠ G132) binding Na(+)
L150 (≠ I134) binding Na(+)
E208 (= E188) binding L-homoserine; mutation to D: Increases KM for aspartate-semialdehyde 48-fold and reduces kcat by 50%.; mutation E->L,Q: Loss of activity.
D219 (= D199) binding L-homoserine; mutation to L: Reduces kcat 150-fold.
K223 (= K203) mutation to V: Loss of activity.

Sites not aligning to the query:

13 binding NAD(+)
15 binding NAD(+)
16 binding NAD(+)
41 binding NAD(+)
309 H→A: Reduces kcat 40-fold. Affects dimer formation.
340 binding NAD(+)

1tveA Homoserine dehydrogenase in complex with 4-(4-hydroxy-3- isopropylphenylthio)-2-isopropylphenol (see paper)
31% identity, 37% coverage: 53:211/434 of query aligns to 67:230/358 of 1tveA

query
sites
1tveA

T

T

G

K

S

T

T

L

P

P

I

L

T

D

A

D

D

L

I

I

F

A

D

H

L

L

V

K

N

T

N

S

P

P

E

K

I

P

D

V

I

I

V

L

V

V

E

D

L

N

I

T

G
x
S

G

S

Y

A

T

Y

V

I

A

A

K

G

E

-

L

F

V

Y

L

T

K

K

A

F

I

V

E

E

N

N

G

G

K

I

H

S

V

I

V

A

T

T

A

P

N

N

K
|
K

A

K

L

A

I

F

A

S

-

S

-

D

-

L

V

A

H

T

G

W

N

K

E

A

I

L

F

F

A

S

K

N

A

K

R

P

E

T

K

N

G

G

V

-

I

F

V

V

A

Y

F

H

E

E

A

A

S

T

V

V

A

G

G

A

G

G

I

L

P

P

V

I

I

I

K

S

A

F

I

L

R

R

E

E

G

I

L

I

-

Q

A

T

G

G

N

D

R

E

I

V

N

E

W

K

L

I

A

E

G

G

I

I

I

F

N

S

G

G

T
|
T

G

L

N

S

F

Y

I

I

L

F

T

N

E

E

M

F

R

S

E

T

K

S

G

Q

R

A

T

N

-

D

-

V

-

K

F

F

E

S

D

D

V

V

L

V

K

K

E

V

A

A

Q

K

E

K

L

L

G

G

Y

Y

A

T

E

E

A

P

D

D

P

P

T

R

F

D

D
|
D

V

L

E

N

G

G

I

L

D
|
D

A

V

A

A

H

R

K
|
K

L

V

T

T

I

I

L

V

A

G

S

R

I

I

A

S

active site: D218 (= D199), K222 (= K203)
binding 4-(4-hydroxy-3-isopropylphenylthio)-2-isopropylphenol: S93 (≠ G79), K116 (= K103), T175 (= T159), D213 (= D194), D218 (= D199)

1q7gA Homoserine dehydrogenase in complex with suicide inhibitor complex NAD-5-hydroxy-4-oxonorvaline (see paper)
31% identity, 37% coverage: 53:211/434 of query aligns to 67:230/358 of 1q7gA

query
sites
1q7gA

T

T

G

K

S

T

T

L

P

P

I

L

T

D

A

D

D

L

I

I

F

A

D

H

L

L

V

K

N

T

N

S

P

P

E

K

I

P

D

V

I

I

V

L

V

V

E

D

L
x
N

I
x
T

G
x
S

G

S

Y

A

T

Y

V
x
I

A

A

K

G

E

-

L

F

V

Y

L

T

K

K

A

F

I

V

E

E

N

N

G

G

K

I

H

S

V

I

V

A

T

T

A
x
P

N

N

K
|
K

A

K

L

A

I

F

A

S

-

S

-

D

-

L

V

A

H

T

G

W

N

K

E

A

I

L

F

F

A

S

K

N

A

K

R

P

E

T

K

N

G

G

V

-

I

F

V

V

A

Y

F

H

E

E

A
|
A

S

T

V

V

A

G

G

A

G

G

I

L

P

P

V

I

I

I

K

S

A

F

I

L

R

R

E

E

G

I

L

I

-

Q

A

T

G

G

N

D

R

E

I

V

N

E

W

K

L

I

A

E

G

G

I

I

I

F

N
x
S

G

G

T

T

G

L

N

S

F

Y

I

I

L

F

T

N

E

E

M

F

R

S

E

T

K

S

G

Q

R

A

T

N

-

D

-

V

-

K

F

F

E

S

D

D

V

V

L

V

K

K

E

V

A

A

Q

K

E

K

L

L

G

G

Y

Y

A

T

E

E

A

P

D

D

P

P

T

R

F

D

D

D

V

L

E

N

G

G

I

L

D
|
D

A

V

A

A

H

R

K
|
K

L

V

T

T

I

I

L

V

A

G

S

R

I

I

A

S

active site: D218 (= D199), K222 (= K203)
binding nicotinamide-adenine-dinucleotide-5-hydroxy-4-oxonorvaline: N91 (≠ L77), T92 (≠ I78), S93 (≠ G79), I97 (≠ V83), P114 (≠ A101), K116 (= K103), A143 (= A128), S173 (≠ N157), K222 (= K203)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide-5-hydroxy-4-oxonorvaline: 13, 14, 15, 39, 338, 343

1ebuD Homoserine dehydrogenase complex with NAD analogue and l-homoserine (see paper)
31% identity, 37% coverage: 53:211/434 of query aligns to 67:230/358 of 1ebuD

query
sites
1ebuD

T

T

G

K

S

T

T

L

P

P

I

L

T

D

A

D

D

L

I

I

F

A

D

H

L

L

V

K

N

T

N

S

P

P

E

K

I

P

D

V

I

I

V

L

V

V

E

D

L
x
N

I

T

G
x
S

G

S

Y

A

T

Y

V

I

A

A

K

G

E

-

L

F

V

Y

L

T

K

K

A

F

I

V

E

E

N

N

G

G

K

I

H

S

V

I

V

A

T

T

A

P

N

N

K
|
K

A

K

L

A

I

F

A

S

-

S

-

D

-

L

V

A

H

T

G

W

N

K

E

A

I

L

F

F

A

S

K

N

A

K

R

P

E

T

K

N

G

G

V

-

I

F

V

V

A

Y

F

H

E

E

A

A

S

T

V

V

A

G

G

A

G

G

I

L

P

P

V

I

I

I

K

S

A

F

I

L

R

R

E

E

G

I

L

I

-

Q

A

T

G

G

N

D

R

E

I

V

N

E

W

K

L

I

A

E

G

G

I

I

I

F

N

S

G

G

T

T

G

L

N

S

F

Y

I

I

L

F

T

N

E

E

M

F

R

S

E

T

K

S

G

Q

R

A

T

N

-

D

-

V

-

K

F

F

E

S

D

D

V

V

L

V

K

K

E

V

A

A

Q

K

E

K

L

L

G

G

Y

Y

A

T

E

E

A

P

D

D

P

P

T

R

F

D

D

D

V

L

E

N

G

G

I

L

D
|
D

A

V

A

A

H

R

K
|
K

L

V

T

T

I

I

L

V

A

G

S

R

I

I

A

S

active site: D218 (= D199), K222 (= K203)
binding 3-aminomethyl-pyridinium-adenine-dinucleotide: N91 (≠ L77), S93 (≠ G79), K116 (= K103)

Sites not aligning to the query:

binding 3-aminomethyl-pyridinium-adenine-dinucleotide: 11, 12, 13, 14, 15, 39, 40, 343

1ebfA Homoserine dehydrogenase from s. Cerevisiae complex with NAD+ (see paper)
31% identity, 37% coverage: 53:211/434 of query aligns to 67:230/358 of 1ebfA

query
sites
1ebfA

T

T

G

K

S

T

T

L

P

P

I

L

T

D

A

D

D

L

I

I

F

A

D

H

L

L

V

K

N

T

N

S

P

P

E

K

I

P

D

V

I

I

V

L

V

V

E

D

L

N

I
x
T

G
x
S

G

S

Y

A

T

Y

V

I

A

A

K

G

E

-

L

F

V

Y

L

T

K

K

A

F

I

V

E

E

N

N

G

G

K

I

H

S

V

I

V

A

T

T

A
x
P

N

N

K

K

A

K

L

A

I

F

A

S

-

S

-

D

-

L

V

A

H

T

G

W

N

K

E

A

I

L

F

F

A

S

K

N

A

K

R

P

E

T

K

N

G

G

V

-

I

F

V

V

A

Y

F

H

E

E

A

A

S

T

V

V

A

G

G

A

G

G

I

L

P

P

V

I

I

I

K

S

A

F

I

L

R

R

E

E

G

I

L

I

-

Q

A

T

G

G

N

D

R

E

I

V

N

E

W

K

L

I

A

E

G

G

I

I

I

F

N

S

G

G

T

T

G

L

N

S

F

Y

I

I

L

F

T

N

E

E

M

F

R

S

E

T

K

S

G

Q

R

A

T

N

-

D

-

V

-

K

F

F

E

S

D

D

V

V

L

V

K

K

E

V

A

A

Q

K

E

K

L

L

G

G

Y

Y

A

T

E

E

A

P

D

D

P

P

T

R

F

D

D

D

V

L

E

N

G

G

I

L

D
|
D

A

V

A

A

H

R

K
|
K

L

V

T

T

I

I

L

V

A

G

S

R

I

I

A

S

active site: D218 (= D199), K222 (= K203)
binding nicotinamide-adenine-dinucleotide: T92 (≠ I78), S93 (≠ G79), P114 (≠ A101)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 10, 12, 13, 14, 15, 39, 40

Query Sequence

>WP_020308877.1 NCBI__GCF_000013785.1:WP_020308877.1
MKPVKVGICGLGTVGGGTFNVLKRNAEEIARRAGRGIEVAQIATRHPNSKCDTGSTPITA
DIFDLVNNPEIDIVVELIGGYTVAKELVLKAIENGKHVVTANKALIAVHGNEIFAKAREK
GVIVAFEASVAGGIPVIKAIREGLAGNRINWLAGIINGTGNFILTEMREKGRTFEDVLKE
AQELGYAEADPTFDVEGIDAAHKLTILASIAFGIPLQFDKAYTEGIARLTTADVNYAEAL
GYRIKHLGVARRTEAGIELRVHPTLIPADRLIANVNGVMNAVMVNGDAVGSTLYYGAGAG
MEPTASAVVADVVDVTRALTTDPNNRVPHLAFQPDSLSDHPILPITACESAYYLRIQAKD
HPGVLAQVATILSERGINIESIMQKEAEVQDGLVPVILVTHRVVEQRIDDAIAALERLDD
VVDKVVRIRVEQLN

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory