SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_020561754.1 NCBI__GCF_000372865.1:WP_020561754.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P05041 Aminodeoxychorismate synthase component 1; ADC synthase; ADCS; 4-amino-4-deoxychorismate synthase component 1; EC 2.6.1.85 from Escherichia coli (strain K12) (see 4 papers)
48% identity, 99% coverage: 1:454/458 of query aligns to 1:453/453 of P05041

query
sites
P05041

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S36 (= S35) binding L-tryptophan
E258 (= E259) mutation to A: The reaction is extremely slow.; mutation to D: The reaction is extremely slow.
K274 (= K275) mutation to A: Absence of covalent intermediate. Addition of ammonia allows the formation of the covalent intermediate and shows that ammonia can replace the function of K-274. Reduced catalytic efficiency.; mutation to R: Absence of covalent intermediate.; mutation to R: Reduced catalytic efficiency.
G275 (= G276) mutation to S: Catalytically inactive for both the glutamine-dependent and ammonia-dependent reactions and fails to interact with PabA.
R311 (= R312) mutation to K: Catalytically active in the NH3-dependent, but inactive for the glutamine-dependent reactions and fails to complex with PabA.
R316 (≠ K317) mutation to H: Catalytically inactive for both the glutamine-dependent and ammonia-dependent reactions and fails to interact with PabA.
S322 (= S323) mutation to T: Complete loss of aminodeoxychorismate synthase activity.
H339 (= H340) mutation to W: Catalytically inactive for both the glutamine-dependent and ammonia-dependent reactions and fails to interact with PabA.

1k0eA The crystal structure of aminodeoxychorismate synthase from formate grown crystals (see paper)
48% identity, 97% coverage: 11:454/458 of query aligns to 9:437/437 of 1k0eA

query
sites
1k0eA

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active site: E256 (= E259), K272 (= K275), E286 (= E303), H323 (= H340), S350 (= S367), W374 (≠ Y391), R394 (= R411), G410 (= G427), E423 (= E440), K427 (= K444)
binding tryptophan: L32 (= L33), H33 (≠ D34), S34 (= S35), Y41 (≠ Q42), F44 (≠ Y45), P238 (= P241), F239 (= F242), S240 (= S243)

1k0gA The crystal structure of aminodeoxychorismate synthase from phosphate grown crystals (see paper)
45% identity, 99% coverage: 1:454/458 of query aligns to 1:420/420 of 1k0gA

query
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1k0gA

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G

K

R

T

V

C

A

K

V

K

A

G

G

S

S

V

V

K

K

V

V

P

P

R

E

I

L

F

F

A

V

V

-

E

-

T

-

Y

-

A

-

T

-

V

-

H

-

L

L

V

V

S

S

T

T

I

I

T

T

G

A

W

Q

L

L

A

P

D

E

D

Q

R

L

H

H

A

A

L

S

D

D

L

L

K

R

H

A

C

A

F

F

P

P

G

G

S
|
S

I

I

T

T

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

E

V

I

I

I

E
x
D

E

E

L

L

E

E

P

P

N

Q

R
|
R

R

R

G

N

I

A

Y
x
W

C

C

G

G

S

S

I

I

G

G

Y

Y

I

L

G

S

F

F

D

C

G

G

N

N

M

M

D

D

S

T

N

S

I

I

A

T

I

I

R
|
R

T

T

L

L

V

T

H

A

S

I

G

N

G

G

T

Q

I

I

R

F

F

C

W

S

A

A

G

G

G
|
G

I

I

V

V

N

A

D

D

S

S

E

Q

V

E

D

E

D

A

E

E

Y

Y

Q

Q

E
|
E

C

T

F

F

D

D

K
|
K

A

V

A

N

A

R

M

I

L

L

D

K

L

Q

L

L

Q

E

R

K

active site: E258 (= E259), K274 (= K275), E278 (= E303), S333 (= S367), W357 (≠ Y391), R377 (= R411), G393 (= G427), E406 (= E440), K410 (= K444)
binding phosphate ion: D113 (= D109), R116 (= R112), D347 (≠ E381), R353 (= R387)
binding tryptophan: L34 (= L33), H35 (≠ D34), S36 (= S35), Y43 (≠ Q42), S44 (≠ G43), F46 (≠ Y45), P240 (= P241), F241 (= F242), S242 (= S243)

1k0gB The crystal structure of aminodeoxychorismate synthase from phosphate grown crystals (see paper)
45% identity, 99% coverage: 1:452/458 of query aligns to 1:415/415 of 1k0gB

query
sites
1k0gB

M

M

S

K

P

T

L

L

N

S

Q

P

F

A

M

V

S

I

P

T

L

L

P

L

Y

W

F

R

A

Q

D

D

S

A

A

A

D

E

L

F

-

Y

F

F

S

S

A

R

I

L

A

S

G

H

M

L

P

P

W

W

S

A

V

M

F

L

L
|
L

D
x
H

S
|
S

G

G

Y

Y

P

A

Y

D

S

H

Q

P

Q
x
Y

G
x
S

R
|
R

Y
x
F

D

D

I

I

I

V

A

V

A

A

D

E

P

P

V

I

C

C

I

T

L

L

V

T

T

T

H

F

G

G

D

K

E

E

T

T

V

V

I

V

T

S

R

E

G

S

G

E

K

K

S

R

V

T

I

T

S

T

K

T

D

D

P

P

F

L

D

Q

L

V

V

L

K

Q

Q

Q

Q

V

L

L

-

D

-

R

-

A

-

D

-

I

L

R

P

P

A

T

H

H

C

-

Q

-

G

N

D

E

T

D

L

L

P

P

F

F

K

Q

G

G

A

A

I

L

G

G

Y

L

F

F

S

G

Y
|
Y

D
|
D

L

L

V

G

R
|
R

R

R

L

F

E

E

H

S

L

L

S

P

E

E

I

I

A

A

V

E

D

Q

A

D

E

I

H

V

I

L

P

P

E

D

M

M

A

A

V

V

G

G

I

I

Y

Y

D

D

W

W

A

A

V

L

V

I

V

V

D

D

H

H

H

Q

C

R

R

H

K

T

S

V

Y

S

L

L

V

L

G

S

Q

H

N

N

-

D

-

V

C

N

D

A

M

R

Q

R

K

A

W

W

L

L

K

E

L

-

I

S

E

Q

Q

Q

F

F

S

S

V

-

L

-

P

P

V

Q

E

E

E

D

R

-

G

-

P

-

F

F

R

T

I

L

V

T

G

S

D

D

I

W

R

Q

S

S

N

N

M

M

D

T

K

R

A

E

F

Q

Y

Y

T

G

R

E

A

K

F

F

D

R

R

Q

I

V

K

Q

Q

E

Y

Y

L

L

K

H

E

S

G

G

D

D

C

C

Y

Y

Q

Q

V

V

N

N

F

L

S

A

Q

Q

R

R

F

F

V

H

A

A

D

T

C

Y

E

S

G

G

D

D

P

E

W

W

T

Q

A

A

Y

F

K

L

A

Q

L

L

R

N

E

Q

L

A

N

N

A

R

A

A

P
|
P

F
|
F

S

S

C

A

Y

F

L

L

N

R

L

L

P

E

E

Q

V

G

Q

A

I

I

L

L

S

S

T

L

S

S

P

P

E
|
E

R

R

F

F

L

I

K

L

L

C

T

D

D

N

G

S

L

E

V

I

E

Q

T

T

K

R

P

P

I

I

K
|
K

G

G

T

-

R

-

P

-

R

-

K

-

R

-

D

-

Q

-

A

-

E

-

D

-

R

-

Q

-

I

-

E

-

I

-

L

-

E

-

H

-

S

-

P

-

K

-

D

-

R

-

A

A

E
|
E

N

N

L

L

M

M

I

I

V

V

D

D

L

L

L

M

R

R

N

N

D

D

I

I

S

G

K

R

T

V

C

A

K

V

K

A

G

G

S

S

V

V

K

K

V

V

P

P

R

E

I

L

F

-

A

-

V

-

E

-

T

-

Y

-

A

-

T

-

V

-

H

-

L

L

V

V

S

S

T

T

I

I

T

T

G

A

W

Q

L

L

A

P

D

E

D

Q

R

L

H

H

A

A

L

S

D

D

L

L

K

R

H

A

C

A

F

F

P

P

G

G

S
|
S

I

I

T

T

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

E

V

I

I

I

E
x
D

E

E

L

L

E

E

P

P

N

Q

R
|
R

R

R

G

N

I

A

Y
x
W

C

C

G

G

S

S

I

I

G

G

Y

Y

I

L

G

S

F

F

D

C

G

G

N

N

M

M

D

D

S

T

N

S

I

I

A

T

I

I

R
|
R

T

T

L

L

V

T

H

A

S

I

G

N

G

G

T

Q

I

I

R

F

F

C

W

S

A

A

G

G

G
|
G

I

I

V

V

N

A

D

D

S

S

E

Q

V

E

D

E

D

A

E

E

Y

Y

Q

Q

E
|
E

C

T

F

F

D

D

K
|
K

A

V

A

N

A

R

M

I

L

L

D

K

L

Q

L

L

active site: E258 (= E259), K274 (= K275), E277 (= E303), S330 (= S367), W354 (≠ Y391), R374 (= R411), G390 (= G427), E403 (= E440), K407 (= K444)
binding phosphate ion: Y112 (= Y108), D113 (= D109), R116 (= R112), D344 (≠ E381), R350 (= R387)
binding tryptophan: L34 (= L33), H35 (≠ D34), S36 (= S35), Y43 (≠ Q42), S44 (≠ G43), R45 (= R44), F46 (≠ Y45), P240 (= P241), F241 (= F242)

7pi1DDD Aminodeoxychorismate synthase component 1
37% identity, 90% coverage: 43:453/458 of query aligns to 39:454/459 of 7pi1DDD

query
sites
7pi1DDD

G
|
G

R

R

Y
|
Y

D

S

I

I

I

A

A

G

A

L

D

D

P

P

V

I

C

A

I

T

L

V

V

K

T

G

H

K

G

D

D

G

E

I

T

T

V

T

I

I

T

K

R

H

G

G

G

D

K

E

S

M

V

L

I

F

S

K

K

E

D

G

D

D

P

P

F

L

D

R

L

A

V

F

K

H

Q

S

-

W

-

F

Q

K

L

T

L

L

P

E

A

T

H

E

C

T

Q

N

G

H

D

E

T

F

L

P

P

D

F

F

K

Q

G

G

A

A

I

I

G

G

Y

F

F

L

S

S

Y

Y

D

D

L

Y

V

A

R

R

R

Y

L

I

E

E

H

N

L

F

S

K

E

M

I

L

A

S

V

L

D

D

A

D

E

L

H

E

I

T

P

P

E

D

M

I

A

Y

V

F

G

L

I

V

Y

F

D

D

W

D

A

I

V

A

V

V

V

Y

D

D

H

H

H

Q

C

E

R

E

K

S

S

L

Y

W

L

L

V

I

G

T

Q

H

N

V

C

N

D

D

M

Q

Q

E

K

T

W

A

-

D

-

V

-

K

L

L

K

S

L

E

I

L

E

E

Q

Q

-

M

-

W

F

L

S

T

V

E

L

L

P

P

V

A

E

V

E

E

R

M

R

K

G

P

P

-

F

-

R

E

I

T

V

A

G

G

D

S

I

F

R

A

S

A

N

P

M

F

D

T

K

E

A

D

F

G

Y

F

T

S

R

Q

A

A

F

V

D

E

R

K

I

I

K

K

Q

Q

Y

Y

L

I

K

A

E

S

G

G

D

D

C

V

Y

F

Q

Q

V

V

N

N

F

L

S

S

Q

I

R

R

F

Q

V

S

A

Q

D

S

C

L

E

S

G

V

D

H

P

P

W

Y

T

Q

A

I

Y

Y

K

K

A

T

L

L

R

R

E

E

L

V

N

N

A

P

A

S

P
|
P

F
x
Y

S
x
M

C

A

Y

Y

L

L

N

E

L

T

P

P

E

D

V

F

Q

Q

I

I

L

I

S

C

T

G

S

S

P

P

E

E

R

L

F

L

L

V

K

S

L

K

T

K

D

G

G

K

L

L

V

L

E

E

T

T

K

R

P

P

I

I

K

A

G

G

T

T

R

R

P

S

R

R

K

G

R

K

D

T

Q

N

A

E

E

E

D

D

R

E

Q

A

Q

L

I

A

E

N

I

E

L

L

E

I

H

H

S

N

P

E

K

K

D

E

R

R

A

A

E

E

N

H

L

V

M

M

I

L

V

V

D

D

L

L

L

E

R

R

N

N

D

D

I

L

S

G

K

R

T

V

C

S

K

R

K

Y

G

G

S

S

V

V

K

R

V

V

P

N

R

E

I

F

F

M

A

A

V

I

E

E

T

K

Y

Y

A

S

T

H

V

V

H

M

H

H

L

I

V

V

S

S

T

N

I

V

T

Q

G

G

W

E

L

L

A

Q

D

D

D

G

R

Y

H

D

A

A

L

V

D

D

L

I

K

H

H

A

C

V

F

F

P

P

G

G

S

T

I

I

T

T

G

G

A

A

P

P

K

K

I

V

R

R

A

T

M

M

E

E

V

I

I

I

E

E

L

L

E

E

P

P

N

T

R

R

G

G

I

L

Y

Y

C

T

G

G

S

S

I

I

G

G

Y

W

I

F

G

G

F

Y

D

N

G

H

N

D

M

L

D
x
Q

S

F

N
|
N

I

I

A

V

I

I

R

R

T

T

L

I

V

Y

H

A

S

T

G

G

T

Q

I

A

R

F

F

M

W

Q

A

S

G

G

G

A

G
|
G

I

V

V

V

N

I

D

D

S

S

E

V

V

P

D

K

D

H

E
|
E

Y

Y

Q

K

E

E

C

S

F

F

D

K

K

K

A

A

A

F

A

A

M

M

L

Q

D

R

L

A

L

L

Q

E

binding magnesium ion: G428 (= G427), E438 (= E437)
binding tryptophan: G39 (= G43), Y41 (= Y45), P242 (= P241), Y243 (≠ F242), M244 (≠ S243), Q406 (≠ D405), N408 (= N407)

Sites not aligning to the query:

binding tryptophan: 33, 34, 35

P28820 Aminodeoxychorismate synthase component 1; ADC synthase; ADCS; 4-amino-4-deoxychorismate synthase component 1; EC 2.6.1.85 from Bacillus subtilis (strain 168) (see paper)
37% identity, 90% coverage: 43:453/458 of query aligns to 41:461/470 of P28820

query
sites
P28820

G

G

R

R

Y

Y

D

S

I

I

I

A

A

G

A

L

D

D

P

P

V

I

C

A

I

T

L

V

V

K

T

G

H

K

G

D

D

G

E

I

T

T

V

T

I

I

T

K

R

H

G

G

G

D

K

E

S

M

V

L

I

F

S

K

K

E

D

G

D

D

P

P

F

L

D

R

L

A

V

F

K

H

Q

S

-

W

-

F

Q

K

L

T

L

L

P

E

A

T

H

E

C

T

Q

N

G

H

D

E

T

F

L

P

P

D

F

F

K

Q

G

G

A

A

I

I

G

G

Y

F

F

L

S

S

Y

Y

D

D

L

Y

V

A

R

R

R

Y

L

I

E

E

H

N

L

F

S

K

E

M

I

L

A

S

V

L

D

D

A

D

E

L

H

E

I

T

P

P

E

D

M

I

A

Y

V

F

G

L

I

V

Y

F

D

D

W

D

A

I

V

A

V

V

V

Y

D

D

H

H

H

Q

C

E

R

E

K

S

S

L

Y

W

L

L

V

I

G

T

-

H

-

V

-

N

-

G

-

S

-

D

-

Q

Q

E

N

T

C

A

D

D

M

V

Q

K

K

-

W

-

L

L

K

S

L

E

I

L

E

E

Q

Q

-

M

-

W

F

L

S

T

V

E

L

L

P

P

-

A

V

V

E

T

E

S

R

R

R

E

G

M

P

K

F

P

R

E

I

T

V

A

G

G

D

S

I

F

R

A

S

A

N

P

M

F

D

T

K

E

A

D

F

G

Y

F

T

S

R

Q

A

A

F

V

D

E

R

K

I

I

K

K

Q

Q

Y

Y

L

I

K

A

E

S

G

G

D

D

C

V

Y

F

Q

Q

V

V

N

N

F

L

S

S

Q

I

R

R

F

Q

V

S

A

Q

D

S

C

L

E

S

G

V

D

H

P

P

W

Y

T

Q

A

I

Y

Y

K

K

A

T

L

L

R

R

E

E

L

V

N

N

A

P

A

S

P

P

F

Y

S

M

C

A

Y

Y

L

L

N

E

L

T

P

P

E

D

V

F

Q

Q

I

I

L

I

S

C

T

G

S

S

P

P

E

E

R

L

F

L

L

V

K

S

L

K

T

K

D

G

G

K

L

L

V

L

E

E

T

T

K

R

P

P

I

I

K
x
A

G

G

T

T

R

R

P

S

R

R

K

G

R

K

D

T

Q

N

A

E

E

E

D

D

R

E

Q

A

Q

L

I

A

E

N

I

E

L

L

E

I

H

H

S

N

P

E

K

K

D

E

R

R

A

A

E

E

N

H

L

V

M

M

I

L

V

V

D

D

L

L

L

E

R

R

N

N

D

D

I

L

S

G

K

R

T

V

C

S

K

R

K

Y

G

G

S

S

V

V

K

R

V

V

P

N

R

E

I

F

F

M

A

A

V

I

E

E

T

K

Y

Y

A

S

T

H

V

V

H

M

H

H

L

I

V

V

S

S

T

N

I

V

T

Q

G

G

W

E

L

L

A

Q

D

D

D

G

R

Y

H

D

A

A

L

V

D

D

L

I

K

H

H

A

C

V

F

F

P

P

G

G

S

T

I

I

T

T

G

G

A

A

P

P

K

K

I

V

R

R

A

T

M

M

E

E

V

I

I

I

E

E

L

L

E

E

P

P

N

T

R

R

G

G

I

L

Y

Y

C

T

G

G

S

S

I

I

G

G

Y

W

I

F

G

G

F

Y

D

N

G

H

N

D

M

L

D

Q

S

F

N

N

I

I

A

V

I

I

R

R

T

T

L

I

V

Y

H

A

S

T

G

G

T

Q

I

A

R

F

F

M

W

Q

A

S

G

G

G

A

G

G

I

V

V

V

N

I

D

D

S

S

E

V

V

P

D

K

D

H

E

E

Y

Y

Q

K

E

E

C

S

F

F

D

K

K

K

A

A

A

F

A

A

M

M

L

Q

D

R

L

A

L

L

Q

E

A283 (≠ K275) mutation to I: Complete loss of aminodeoxychorismate synthase activity.; mutation to K: Absence of covalent intermediate.; mutation to V: Complete loss of aminodeoxychorismate synthase activity.

A0QX93 Anthranilate synthase component 1; AS; ASI; EC 4.1.3.27 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
36% identity, 95% coverage: 14:450/458 of query aligns to 49:513/524 of A0QX93

query
sites
A0QX93

F

L

A

A

D

D

S

S

A

E

D

T

L

P

F

L

S

S

A

A

-

Y

-

R

-

K

-

L

I

A

A

A

G

N

M

R

P

P

W

G

S

T

V

F

F

L

L

L

D

E

S

S

G

A

Y

E

P

N

Y

G

S

R

Q

S

Q

W

G

S

R

R

Y

W

D

S

I

F

I

I

A

G

A

A

D

G

-

A

P

P

V

S

C

A

I

L

L

T

V

V

T

R

H

D

G

N

D

A

E

A

T

A

V

W

I

L

T

G

R

T

G

A

G

P

K

E

S

G

V

A

I

P

S

S

K

G

D

G

D

D

P

P

F

L

D

D

L

A

V

L

K

R

Q

A

Q

T

L

L

-

D

-

L

L

L

P

K

A

T

H

E

C

A

Q

M

G

A

D

G

T

L

L

P

P

P

F

L

K

S

G

S

G

G

A

L

I

V

G

G

Y

F

F

F

S

A

Y

Y

D

D

L

M

V

V

R

R

L

L

E

E

H

R

L

L

S

P

E

E

I

L

A

A

V

V

D

D

A

D

E

L

H

G

I

L

P

P

E

D

M

M

A

L

V

L

G

L

I

L

Y

A

D

T

W

D

A

I

V

A

V

V

D

D

H

H

C

E

R

G

K

T

S

I

Y

T

L

L

V

I

G

A

Q

N

N

A

C

V

D

N

M

W

Q

N

-

G

-

T

-

D

-

E

-

R

-

V

K

D

W

W

-

A

-

Y

-

D

-

A

-

V

-

A

-

R

L

L

K

D

L

V

I

M

E

T

Q

K

F

A

S

L

V

G

L

Q

P

P

V

L

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T

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R

A

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V

G

A

P

T

F

F

-

S

R

R

I

P

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A

G

P

D

D

I

H

R

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S

A

N

Q

M

R

D

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K

M

A

E

F

E

Y

Y

T

T

R

E

A

I

F

V

D

D

R

K

I

L

K

V

Q

G

Y

D

L

I

K

E

E

A

G

G

D

E

C

A

Y

F

Q

Q

V

V

N

V

F

P

S

S

Q

Q

R

R

F

F

V

E

A

M

D

D

C

T

E

A

G

A

D

D

P

P

W

L

T

D

A

V

Y

Y

K

R

A

I

L

L

R

R

E

V

L

T

N

N

A

P

A

S

P

P

F

Y

S

M

C

Y

Y

L

L

L

N

N

L

I

P

P

E

D

V

A

-

D

-

G

-

L

-

D

-

F

Q

S

I

I

L

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S

G

T

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S

S

P

P

E

E

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A

F

L

L

V

K

T

L

V

T

K

D

D

G

G

L

R

V

A

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T

T

T

K

H

P

P

I

I

K

A

G

G

T

T

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R

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W

R

R

K

G

R

A

D

T

Q

E

A

E

E

E

D

D

R

V

Q

L

I

E

E
x
K

I

E

L

L

E

L

H

A

S

D

P

E

K

K

D

E

R

R

A

A

E

E

N

H

L

L

M

M

I

L

V

V

D

D

L

L

L

G

R

R

N

N

D

D

I

L

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K

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C

C

K

R

K

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G

G

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V

K

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R

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Y

F

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A

H

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T

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Y

Y

A

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H

V

V

H

M

H

H

L

L

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V

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S

T

T

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T

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G

G

W

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L

L

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A

D

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D

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K

H

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A

A

L

L

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D

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A

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K

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A

C

C

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F

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P

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A

G

G

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L

T

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G

A

A

P

P

K

K

I

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R

R

A

A

M

M

E

E

V

L

I

I

E

E

L

V

E

E

P

K

N

T

R

R

G

G

I

L

Y

Y

C

G

G

G

S

V

I

V

G

G

Y

Y

I

L

G

D

F

F

D

A

G

G

N

N

M

A

D

D

S

F

N

A

I

I

A

A

I

I

R

R

T

T

L

A

V

L

H

M

S

R

G

N

G

G

T

T

I

A

R

Y

F

V

W

Q

A

A

G

G

I

V

V

V

N

A

D

D

S

S

E

N

V

G

D

P

D

Y

E

E

Y

Y

Q

T

E

E

C

A

F

A

D

N

K

K

A

A

A

R

A

A

M

V

L

L

D

N

K355 (≠ E292) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

7bvdA Anthranilate synthase component i (trpe)[mycolicibacterium smegmatis]
36% identity, 95% coverage: 14:450/458 of query aligns to 29:488/499 of 7bvdA

query
sites
7bvdA

F

L

A

A

D

D

S

S

A

E

D

T

L

P

F

L

S

S

A

A

-

Y

-

R

-

K

-

L

I

A

A

A

G

N

M

R

P

P

W

G

S

T

V

F

F

L

L

L

D

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S

S

G

A

Y

E

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N

Y

R

S

S

Q

W

Q

S

G

R

R

W

Y

S

D

F

I

I

I

G

A

A

A

G

D

A

P

P

V

S

C

A

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L

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V

V

T

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H

D

G

N

D

A

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A

T

A

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W

I

L

T

G

R

T

G

A

G

P

K

E

S

G

V

A

I

P

S
|
S

K
x
G

D

G

D

D

P

P

F

L

D

D

L
x
A

V

L

K

R

Q

A

Q

T

L

L

-

D

-

L

L

L

P

K

A

T

H

E

C

A

Q

M

G

A

D

G

T

L

L

P

P

P

F

L

K

S

G

S

G

G

A

L

I

V

G

G

Y

F

F

F

S

A

Y

Y

D

D

L

M

V

V

R

R

L

L

E

E

H

R

L

L

S

P

E

E

I

L

A

A

V

V

D

D

A

D

E

L

H

G

I

L

P

P

E

D

M

M

A

L

V

L

G

L

I

L

Y

A

D

T

W

D

A

I

V

A

V

V

D

D

H

H

C

E

R

G

K

T

S

I

Y

T

L

L

V

I

G

A

Q

N

N

A

C

V

D

N

M

W

Q

N

-

G

-

T

-

D

-

E

-

R

-

V

K

D

W

W

-

A

-

Y

-

D

-

A

-

V

-

A

-

R

L

L

K

D

L

V

I

M

E

T

Q

K

F

A

S

L

V

G

L

Q

P

P

V

L

E

T

E

S

R

A

R

V

G

A

P

T

F

F

-

S

R

R

I

P

V

A

G

P

D

D

I

H

R

R

S

A

N

Q

M

R

D

T

K

M

A

E

F

E

Y

Y

T

T

R

E

A

I

F

V

D

D

R

K

I

L

K

V

Q

G

Y

D

L

I

K

E

E

A

G

G

D

E

C

A

Y

F

Q
|
Q

V

V

N

V

F

P

S

S

Q

Q

R

R

F

F

V

E

A

M

D

D

C

T

E

A

G

A

D

D

P

P

W

L

T

D

A

V

Y

Y

K

R

A

I

L

L

R

R

E

V

L

T

N

N

A

P

A

S

P

P

F

Y

S

M

C

Y

Y

L

L

L

N

N

L

I

P

P

E

D

V

A

-

D

-

G

-

L

-

D

-

F

Q

S

I

I

L

V

S

G

T

S

S

S

P

P

E
|
E

R

A

F

L

L

V

K

T

L

V

T

K

D

D

G

G

L

R

V

A

E

T

T

T

K

H

P

P

I

I

K
x
A

G

G

T

T

R

R

P

W

R

R

K

E

R

E

D

D

Q

V

A

L

E

L

D

E

R

K

Q

E

Q

-

I

-

E

-

I

-

L

L

E

L

H

A

S

D

P

E

K

K

D

E

R

R

A

A

E
|
E

N

H

L

L

M

M

I

L

V

V

D

D

L

L

L

G

R

R

N

N

D

D

I

L

S

G

K

R

T

V

C

C

K

R

K

P

G

G

S

T

V

V

K

R

V

V

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D

R

D

I

Y

F

S

A

H

V

I

E

E

T

R

Y

Y

A

S

T

H

V

V

H

M

H
|
H

L

L

V

V

S

S

T

T

I

V

T

T

G

G

W

E

L

L

A

A

D

E

D

D

R

K

H

T

A

A

L

L

D

D

L

A

L

V

K

T

H

A

C

C

F

F

P

P

G

A

G

G

S
x
T

I

L

T

S

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

E

V

L

I

I

E

E

L

V

E

E

P

K

N

T

R

R

G

G

I

L

Y
|
Y

C

G

G

G

S

V

I

V

G

G

Y

Y

I

L

G

D

F

F

D

A

G

G

N

N

M

A

D

D

S

F

N

A

I

I

A

A

I

I

R
|
R

T

T

L

A

V

L

H

M

S

R

G

N

G

G

T

T

I

A

R

Y

F

V

W

Q

A

A

G

G

G
|
G

I

V

V

V

N

A

D

D

S

S

E

N

V

G

D

P

D

Y

E

E

Y

Y

Q

T

E
|
E

C

A

F

A

D

N

K
|
K

A

A

A

R

A

A

M

V

L

L

D

N

active site: Q248 (= Q212), E301 (= E259), A317 (≠ K275), E341 (= E303), H378 (= H340), T405 (≠ S367), Y429 (= Y391), R449 (= R411), G465 (= G427), E478 (= E440), K482 (= K444)
binding pyruvic acid: S93 (= S74), G94 (≠ K75), A100 (≠ L81)

8hx9A Crystal structure of 4-amino-4-deoxychorismate synthase from streptomyces venezuelae with chorismate (see paper)
36% identity, 94% coverage: 24:452/458 of query aligns to 180:631/632 of 8hx9A

query
sites
8hx9A

I

L

A

P

G

G

M

E

P

G

W

T

S

T

V

F

F

W

L
|
L

D
|
D

S
|
S

G

S

Y

S

P

V

Y

L

S

E

Q

G

Q

A

G
x
S

R

R

Y
x
F

D

S

I

F

I

L

A

G

A

D

D

D

-

R

-

G

P

P

V

L

C

A

I

E

L

Y

V

L

T

T

H

Y

-

R

-

V

G

A

D

D

E

G

T

V

V

V

I

S

T

V

R

R

G

G

-

S

-

D

G

G

K

T

S

T

V

T

I

R

S

T

K

R

D

R

D

P

P

F

F

F

D

N

L

Y

V

L

K

E

Q

E

Q

Q

L

L

-

E

-

R

L

V

P

P

A

V

H

A

C

P

Q

E

G

-

D

-

T

-

L

L

P

P

F

F

K

E

G

F

-

N

-

L

G

G

A

Y

I

V

G

G

Y

Y

F

L

S

G

Y

Y

D

E

L

L

V

-

R

-

R

K

L

A

E

E

H

T

L

T

S

G

E

D

I

P

A

A

V

H

D

R

A

S

E

P

H

H

I

-

P

P

E

D

M

A

A

A

V

F

G

L

I

F

Y

A

D

D

W

R

A

A

V

I

V

A

V

L

D

D

H

H

H

Q

C

E

R

G

K

C

S

C

Y

Y

L

L

V

L

-

A

-

L

-

D

-

R

G

G

Q

H

N

D

C

D

D

G

M

A

Q

R

K

A

W

W

L

L

K

R

-

E

L

T

I

A

E

E

Q

T

F

L

S

T

V

G

L

L

P

A

V

V

E

R

E

A

R

P

G

T

P

P

F

A

R

M

I

V

V

F

G

G

D

I

-

P

-

E

-

A

-

G

-

F

-

G

-

P

-

L

I

A

R

R

S

A

N

R

M

H

D

D

K

K

A

D

F

A

Y

Y

T

L

R

K

A

R

F

I

D

D

R

E

I

C

K

L

Q

K

Y

E

L

I

K

R

E

N

G

G

D

E

C

S

Y

Y

Q

E

V

I

N

C

F

L

S

T

Q

N

R

M

F

V

V

T

A

A

D

P

C

T

E

E

G

A

D

T

P

A

W

L

T

P

A

L

Y

Y

K

S

A

A

L

L

R

R

E

A

L

I

N

S

A

P

A

V

P
|
P

F
x
Y

S
x
G

C

A

Y

L

L

L

N

E

L

F

P

P

E

E

V

L

Q

S

I

V

L

L

S

S

T

A

S

S

P

P

E

E

R

R

F

F

L

L

K

T

L

I

-

G

T

A

D

D

G

G

L

G

V

V

E

E

T

S

K

K

P

P

I
|
I

K
|
K

G
|
G

T
|
T

R

R

P

P

R

R

K

G

R

G

D

T

Q

A

A

E

E

E

D

D

R

E

Q

R

Q

L

I

R

E

A

I

D

L

L

E

A

H

G

S

R

P

E

K

K

D

D

R

R

A

A

E

E

N

N

L

L

M

M

I

I

V

V

D

D

L

L

L

V

R

R

N

N

D

D

I

L

S

N

K

S

T

V

C

C

K

A

K

I

G

G

S

S

V

V

K

H

V

V

P

P

R

R

I

L

F

F

A

E

V

V

E

E

T

T

Y

Y

A

A

T

P

V

V

H

H

H

Q

L

L

V

V

S

S

T

T

I

I

T

R

G

G

W

R

L

L

A

R

D

P

D

G

R

T

H

S

A

T

L

A

D

A

L

C

L

V

K

R

H

A

C

A

F

F

P

P

G

G

S

S

I
x
M

T

T

G

G

A

A

P

P

K

K

I

K

R

R

A

T

M

M

E

E

V

I

I

I

E

D

E

R

L

L

E

E

P

E

N

G

R

P

R

R

G

G

I

V

Y
|
Y

C

S

G

G

S

A

I
x
L

G
|
G

Y

W

I

F

G

A

F

L

D

S

G

G

N

A

M

A

D

D

S

L

N

S

I

I

A

V

I

I

R
|
R

T

T

L

I

V

V

H

L

S

A

G

D

G

G

T

Q

I

A

R

E

F

F

W

G

A
x
V

G
|
G

G
x
A

I

I

V

V

N

S

D

L

S

S

E

D

V

Q

D

E

E

E

Y

F

Q

T

E
|
E

C

T

F

V

D

V

K
|
K

A

A

A

R

A

A

M

M

L

V

D

T

L

A

L

L

binding (3R,4R)-3-[(1-carboxyethenyl)oxy]-4-hydroxycyclohexa-1,5-diene-1-carboxylic acid: I453 (= I274), K454 (= K275), G455 (= G276), T456 (= T277), M547 (≠ I368), Y570 (= Y391), R590 (= R411), V603 (≠ A424), G604 (= G425), G605 (= G426), A606 (≠ G427), E619 (= E440), K623 (= K444)
binding tryptophan: L189 (= L33), D190 (= D34), S191 (= S35), S199 (≠ G43), F201 (≠ Y45), P419 (= P241), Y420 (≠ F242), G421 (≠ S243), L574 (≠ I395), G575 (= G396)

Sites not aligning to the query:

binding magnesium ion: 134, 135

8hx8A Crystal structure of 4-amino-4-deoxychorismate synthase from streptomyces venezuelae co-crystallized with chorismate (see paper)
36% identity, 94% coverage: 24:452/458 of query aligns to 222:670/673 of 8hx8A

query
sites
8hx8A

I

L

A

P

G

G

M

E

P

G

W

T

S

T

V

F

F

W

L
|
L

D
|
D

S
|
S

G

S

Y

S

P

V

Y

L

S

E

Q

G

Q

A

G
x
S

R

R

Y
x
F

D

S

I

F

I

L

A

G

A

D

D

D

-

R

-

G

P

P

V

L

C

A

I

E

L

Y

V

L

T

T

H

Y

-

R

-

V

G

A

D

D

E

G

T

V

V

V

I

S

T

V

R

R

G

G

-

S

-

D

G

G

K

T

S

T

V

T

I

R

S

T

K

R

D

R

D

P

P

F

F

F

D

N

L

Y

V

L

K

E

Q

E

Q

Q

L

L

-

E

-

R

L

V

P

P

A

V

H

A

C

P

Q

E

G

-

D

-

T

-

L

L

P

P

F

F

K

E

G

F

-

N

-

L

G

G

A

Y

I

V

G

G

Y

Y

F

L

S

G

Y

Y

D

E

L

L

V

-

R

-

R

K

L

A

E

E

H

T

L

T

S

G

E

D

I

P

A

A

V

H

D

R

A

S

E

P

H

H

I

-

P

P

E

D

M

A

A

A

V

F

G

L

I

F

Y

A

D

D

W

R

A

A

V

I

V

A

V

L

D

D

H

H

H

Q

C

E

R

G

K

C

S

C

Y

Y

L

L

V

L

-

A

-

L

-

D

-

R

G

G

Q

H

N

D

C

D

D

G

M

A

Q

R

K

A

W

W

L

L

K

R

-

E

L

T

I

A

E

E

Q

T

F

L

S

T

V

G

L

L

P

A

V

V

E

R

E

A

-

P

-

A

R

E

R

P

G

T

P

P

F

A

R

M

I

V

V

F

G

G

D

A

-

G

-

F

-

G

-

P

-

L

I

A

R

R

S

A

N

R

M

H

D

D

K

K

A

D

F

A

Y

Y

T

L

R

K

A

R

F

I

D

D

R

E

I

C

K

L

Q

K

Y

E

L

I

K

R

E

N

G

G

D

E

C

S

Y

Y

Q

E

V

I

N

C

F

L

S

T

Q

N

R

M

F

V

V

T

A

A

D

P

C

T

E

E

G

A

D

T

P

A

W

L

T

P

A

L

Y

Y

K

S

A

A

L

L

R

R

E

A

L

I

N

S

A

P

A

V

P
|
P

F
x
Y

S
x
G

C

A

Y

L

L

L

N

E

L

F

P

P

E

E

V

L

Q

S

I

V

L

L

S

S

T

A

S

S

P

P

E

E

R

R

F

F

L

L

K

T

L

I

-

G

T

A

D

D

G

G

L

G

V

V

E

E

T

S

K

K

P

P

I

I

K

K

G

G

T

T

R

R

P

P

R

R

K

G

R

G

D

T

Q

A

A

E

E

E

D

D

R

E

Q

R

Q

L

I

R

E

A

I

D

L

L

E

A

H

G

S

R

P

E

K

K

D

D

R

R

A

A

E
|
E

N

N

L

L

M

M

I

I

V

V

D

D

L

L

L

V

R

R

N

N

D

D

I

L

S

N

K

S

T

V

C

C

K

A

K

I

G

G

S

S

V

V

K

H

V

V

P

P

R

R

I

L

F

F

A

E

V

V

E

E

T

T

Y

Y

A

A

T

P

V

V

H

H

H

Q

L

L

V

V

S

S

T

T

I

I

T

R

G

G

W

R

L

L

A

R

D

P

D

G

R

T

H

S

A

T

L

A

D

A

L

C

L

V

K

R

H

A

C

A

F

F

P

P

G

G

S

S

I

M

T

T

G

G

A

A

P

P

K

K

I

K

R

R

A

T

M

M

E

E

V

I

I

I

E

D

E

R

L

L

E

E

P

E

N

G

R

P

R

R

G

G

I

V

Y

Y

C

S

G

G

S

A

I

L

G
|
G

Y

W

I

F

G

A

F

L

D

S

G

G

N

A

M

A

D

D

S

L

N

S

I

I

A

V

I

I

R

R

T

T

L

I

V

V

H

L

S

A

G

D

G

G

T

Q

I

A

R

E

F

F

W

G

A

V

G

G

G

A

I

I

V

V

N

S

D

L

S

S

E

D

V

Q

D

E

E
|
E

Y

F

Q

T

E
|
E

C

T

F

V

D

V

K

K

A

A

A

R

A

A

M

M

L

V

D

T

L

A

L

L

binding magnesium ion: E521 (= E303), E655 (= E437), E658 (= E440)
binding tryptophan: L231 (= L33), D232 (= D34), S233 (= S35), S241 (≠ G43), F243 (≠ Y45), P458 (= P241), Y459 (≠ F242), G460 (≠ S243), G614 (= G396)

Sites not aligning to the query:

binding magnesium ion: 175, 177

5cwaA Structure of anthranilate synthase component i (trpe) from mycobacterium tuberculosis with inhibitor bound (see paper)
35% identity, 95% coverage: 14:450/458 of query aligns to 29:492/505 of 5cwaA

query
sites
5cwaA

F

L

A

A

D

D

S

S

A

E

D

T

L

P

F

L

S

S

A

A

-

Y

-

R

-

K

-

L

I

A

A

A

G

N

M

R

P

P

W

G

S

T

V

F

F

L

L

L

D

E

S

S

G

A

Y

E

P

N

Y

G

S

R

Q

S

Q

W

G

S

R

R

Y

W

D

S

I

F

I

I

A

G

A

A

D

G

-

A

P

P

V

T

C

A

I

L

L

T

V

V

T

R

H

E

G

G

D

Q

E

A

T

V

V

W

I

L

T

G

R

A

G

V

G

P

K

K

S

D

V

A

I

P

S

T

K

G

D

G

D

D

P

P

F

L

D

R

L

A

V

L

K

Q

Q

V

Q

T

L

L

-

E

L

L

P

L

A

A

H

T

C

A

Q

D

G

P

D

G

T

L

L

P

P

P

F

L

K

S

G

G

A

M

I

V

G

G

Y

F

F

F

S

A

Y

Y

D

D

L

M

V

V

R

R

L

L

E

E

H

R

L

L

S

P

E

E

I

R

A

A

V

V

D

D

A

D

E

L

H

C

I

L

P

P

E

D

M

M

A

L

V

L

G

L

I

L

Y

A

D

T

W

D

A

V

V

A

V

V

D

D

H

H

C

E

R

G

K

T

S

I

Y

T

L

L

V

I

G

A

Q

N

N

A

C

V

D

N

M

W

Q

N

-

G

-

T

-

D

-

E

-

R

-

V

K

D

W

W

L

A

-

Y

-

D

K

D

L

A

I

V

E

A

Q

R

F

L

S

D

V

V

L

M

-

T

-

A

-

L

-

G

-

Q

P

P

V

L

E

P

E

S

R

T

R

V

G

A

P

T

F

F

-

S

R

R

I

P

V

E

G

P

D

R

I

H

R

R

S

A

N

Q

M

R

D

T

K

V

A

E

F

E

Y

Y

T

G

R

A

A

I

F

V

D

E

R

Y

I

L

K

V

Q

D

Y

Q

L

I

K

A

E

A

G

G

D

E

C

A

Y

F

Q
|
Q

V

V

N

V

F

P

S

S

Q

Q

R

R

F

F

V

E

A

M

D

D

C

T

E

D

G

V

D

D

P

P

W

I

T

D

A

V

Y

Y

K

R

A

I

L

L

R

R

E

V

L

T

N

N

A

P

A

S

P

P

F

Y

S

M

C

Y

Y

L

L

L

N

Q

L

V

P

P

-

N

-

S

-

D

-

G

-

A

-

V

E

D

V

F

Q

S

I

I

L

V

S

G

T

S

S

S

P

P

E
|
E

R

A

F

L

L

V

K

T

L

V

T

H

D

E

G

G

L

W

V

A

E

T

T

T

K

H

P

P

I

I

K
x
A

G

G

T

T

R

R

P

W

R

R

K

G

R

R

D

T

Q

D

A

D

E

E

D

D

R

V

Q

L

I

E

E

K

I

E

L

L

E

L

H

A

S

D

P

D

K

K

D

E

R

R

A

A

E
|
E

N

H

L

L

M

M

I

L

V

V

D

D

L

L

L

G

R

R

N

N

D

D

I

L

S

G

K

R

T

V

C

C

K

T

K

P

G

G

S

T

V

V

K

R

V

V

P

E

R

D

I

Y

F

S

A

H

V

I

E

E

T

R

Y

Y

A

S

T

H

V

V

H

M

H
|
H

L

L

V

V

S

S

T

T

I

V

T

T

G

G

W

K

L

L

A

G

D

E

D

G

R

R

H

T

A

A

L

L

D

D

L

A

L

V

K

T

H

A

C

C

F

F

P

P

G

A

G

G

S
x
T

I

L

T

S

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

E

V

L

I

I

E

E

L

V

E

E

P

K

N

T

R

R

G

G

I

L

Y
|
Y

C

G

G

G

S

V

I

V

G

G

Y

Y

I

L

G

D

F

F

D

A

G

G

N

N

M

A

D

D

S

F

N

A

I

I

A

A

I
|
I

R
|
R

T

T

L

A

V

L

H

M

S

R

G

N

G

G

T

T

I

A

R

Y

F

V

W

Q

A
|
A

G
|
G

G

G

G
|
G

I

V

V

V

N

A

D

D

S

S

E

N

V

G

D

S

D

Y

E

E

Y

Y

Q

N

E
|
E

C

A

F

R

D

N

K
|
K

A

A

A

R

A

A

M

V

L

L

D

N

active site: Q248 (= Q212), E301 (= E259), A317 (≠ K275), E345 (= E303), H382 (= H340), T409 (≠ S367), Y433 (= Y391), R453 (= R411), G469 (= G427), E482 (= E440), K486 (= K444)
binding 3-{[(1Z)-1-carboxyprop-1-en-1-yl]oxy}-2-hydroxybenzoic acid: Y433 (= Y391), I452 (= I410), A466 (= A424), G467 (= G425), K486 (= K444)

P32068 Anthranilate synthase alpha subunit 1, chloroplastic; Anthranilate synthase component 1-1; Anthranilate synthase component I-1; Protein A-METHYL TRYPTOPHAN RESISTANT 1; Protein JASMONATE-INDUCED DEFECTIVE LATERAL ROOT 1; Protein TRYPTOPHAN BIOSYNTHESIS 5; Protein WEAK ETHYLENE INSENSITIVE 2; EC 4.1.3.27 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
31% identity, 91% coverage: 33:448/458 of query aligns to 116:581/595 of P32068

query
sites
P32068

L

V

D

E

S

P

G

G

Y

S

P

Q

Y

M

S

S

Q

S

Q

V

G

G

R

R

Y

Y

D

S

I

V

A

G

A

A

D

Q

P

P

V

A

C

M

I

E

L

I

V

V

T

A

H

K

G

E

D

N

E

K

T

V

V

I

I

V

T

M

R

D

G

H

G

N

K

N

S

E

V

T

I

M

S

T

K

E

D

E

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D341 (= D226) mutation to N: In trp5-1; insensitive to feedback inhibition by tryptophan and resistance to the herbicide 6-methylanthranilate.

Q94GF1 Anthranilate synthase alpha subunit 1, chloroplastic; OsASA1; EC 4.1.3.27 from Oryza sativa subsp. japonica (Rice) (see paper)
33% identity, 91% coverage: 33:448/458 of query aligns to 100:563/577 of Q94GF1

query
sites
Q94GF1

L

V

D

E

S

Q

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G

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Y

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D323 (= D226) mutation to N: Insensitive to feedback inhibition by tryptophan.

O94582 Probable anthranilate synthase component 1; Anthranilate synthase component I; EC 4.1.3.27 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
32% identity, 91% coverage: 26:444/458 of query aligns to 45:466/489 of O94582

query
sites
O94582

G

G

M

K

P

K

W

Y

S

S

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F

F

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L

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K

K

S390 (≠ T369) modified: Phosphoserine
S392 (≠ A371) modified: Phosphoserine

Sites not aligning to the query:

488 modified: Phosphoserine

P00898 Anthranilate synthase component 1; AS; ASI; EC 4.1.3.27 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 2 papers)
32% identity, 76% coverage: 104:449/458 of query aligns to 148:507/520 of P00898

query
sites
P00898

G

G

Y

L

F

F

S

A

Y

Y

D

D

L

L

V

V

R

A

R

G

L

F

E

E

H

A

L

L

S

P

E

H

I

L

A

E

V

A

D

-

A

G

E

N

H

N

I

C

P

P

E

D

M

Y

A
x
C

V

F

G

Y

I

L

Y

A

D

E

W

T

A

L

V

M

V

V

V

I

D

D

H

H

H

Q

-

K

-

S

-

T

-

R

C

I

R

Q

K

A

S

S

Y

L

L

F

V

T

G

A

Q

S

N

D

C

R

D

E

M

K

Q

Q

K

R

W

L

-

N

-

A

-

R

L

L

K

A

L

Y

I

L

E

S

Q

Q

F

Q

S

L

V

T

L

Q

P

P

V

A

E

P

R

L

R

-

G

-

P

P

F

V

R

T

I

P

V

V

G

P

D

D

I

M

R

R

S

C

N

E

M

C

D

N

K

Q

A

S

-

D

F

A

Y

F

T

G

R

A

A

V

F

V

D

R

R

Q

I

L

K

Q

Q

K

Y

A

L

I

K

R

E

A

G

G

D

E

C

I

Y

F

Q

Q

V

V

N

V

F

P

S

S

Q

R

R

R

F

F

V

S

A

L

D

P

C

C

E

P

G

S

D

-

P

P

W

L

T

A

A

A

Y

Y

K

Y

A

V

L

L

R

K

E

K

L

S

N
|
N

A
x
P

A

S

P

P

F

Y

S
x
M

C
x
F

Y

F

L

M

N

Q

L

D

P

N

E

D

V

F

Q

T

I

L

L

F

S
x
G

T

A

S

S

P

P

E

E

R

S

F

S

L

L

K

K

L

Y

-

D

-

A

T

A

D

S

G

R

L

Q

V

I

E

E

T

I

K

Y

P

P

I

I

K

A

G

G

T

T

R

R

P

P

R

R

K

G

R

R

-

R

-

A

D

D

Q

G

A

T

E

L

D

D

R

R

-

D

-

L

-

D

Q

S

Q

R

I

I

E

E

I

L

-

D

L

M

E

R

H

T

S

D

P

H

K

K

D

E

R

L

A

S

E

E

N

H

L

L

M

M

I

L

V

V

D

D

L

L

L

A

R

R

N

N

D

D

I

L

S

A

K

R

T

I

C

C

K

T

K

P

G

G

S

S

V

R

K

Y

V

V

P

A

R

D

I

L

F

T

A

K

V

V

E

D

T

R

Y

Y

A

S

T

Y

V

V

H

M

H

H

L

L

V

V

S

S

T
x
R

I

V

T

V

G

G

W

E

L

L

A

R

D

H

D

D

R

L

H

D

A

A

L

L

D

H

L

A

L

Y

K

R

H

A

C

C

F

M

P

N

G

M

G

G

S

T

I

L

T

S

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

Q

V

L

I

I

E

A

E

D

L

A

E

E

P

G

N

Q

R

R

G

G

I

S

Y

Y

C

G

G

G

S

A

I

V

G

G

Y

Y

I

F

G

T

F

A

D

H

G
|
G

N

D

M

L

D

D

S

T

N
x
C

I

I

A

V

I

I

R

R

T

S

L

A

V

L

H

V

S

E

G

N

G

G

T

I

I

A

R

T

F

V

W

Q

A

A

G

G

G

A

G

G

I

I

V

V

N

L

D

D

S

S

E

V

V

P

D

Q

D

S

E

E

Y

A

Q

D

E

E

C

T

F

R

D

N

K

K

A

A

A

R

A

A

M

V

L

L

C174 (≠ A131) mutation to Y: Almost no change in feedback control by tryptophan.
N288 (= N238) mutation to D: Decrease in feedback control by tryptophan.
P289 (≠ A239) mutation to L: Decrease in feedback control by tryptophan.
M293 (≠ S243) mutation to T: Complete loss of feedback control by tryptophan.
F294 (≠ C244) mutation to L: Decrease in feedback control by tryptophan.
G305 (≠ S255) mutation to S: Decrease in feedback control by tryptophan.
R402 (≠ T344) mutation to W: Almost no change in feedback control by tryptophan.
G460 (= G402) mutation to D: Almost no change in feedback control by tryptophan.
C465 (≠ N407) mutation to Y: Complete loss of feedback control by tryptophan. 4-fold decrease of affinity binding for chorismate.

Sites not aligning to the query:

39 E→K: Complete loss of feedback control by tryptophan.
40 binding L-tryptophan; S→F: Complete loss of feedback control by tryptophan.
41 A→V: Decrease in feedback control by tryptophan.
50 binding L-tryptophan
128 R→H: Almost no change in feedback control by tryptophan.
515 H→Y: Almost no change in feedback control by tryptophan.

1i1qA Structure of the cooperative allosteric anthranilate synthase from salmonella typhimurium (see paper)
32% identity, 76% coverage: 104:449/458 of query aligns to 144:503/512 of 1i1qA

query
sites
1i1qA

G

G

Y

L

F

F

S

A

Y

Y

D

D

L

L

V

V

R

A

R

G

L

F

E

E

H

A

L

L

S

P

E

H

I

L

A

E

V

A

D

-

A

G

E

N

H

N

I

C

P

P

E

D

M

Y

A

C

V

F

G

Y

I

L

Y

A

D

E

W

T

A

L

V

M

V

V

V

I

D

D

H

H

H

Q

-

K

-

S

-

T

-

R

C

I

R

Q

K

A

S

S

Y

L

L

F

V

T

G

A

Q

S

N

D

C

R

D

E

M

K

Q

Q

K

R

W

L

-

N

-

A

-

R

L

L

K

A

L

Y

I

L

E

S

Q

Q

F

Q

S

L

V

T

L

Q

P

P

V

A

E

P

R

L

R

-

G

-

P

P

F

V

R

T

I

P

V

V

G

P

D

D

I

M

R

R

S

C

N

E

M

C

D

N

K

Q

A

S

-

D

F

A

Y

F

T

G

R

A

A

V

F

V

D

R

R

Q

I

L

K

Q

Q

K

Y

A

L

I

K

R

E

A

G

G

D

E

C

I

Y

F

Q
|
Q

V

V

N

V

F

P

S

S

Q

R

R

R

F

F

V

S

A

L

D

P

C

C

E

P

G

S

D

-

P

P

W

L

T

A

A

A

Y

Y

K

Y

A

V

L

L

R

K

E

K

L

S

N

N

A

P

A

S

P
|
P

F
x
Y

S
x
M

C

F

Y

F

L

M

N

Q

L

D

P

N

E

D

V

F

Q

T

I

L

L

F

S

G

T

A

S

S

P

P

E
|
E

R

S

F

S

L

L

K

K

L

Y

-

D

-

A

T

A

D

S

G

R

L

Q

V

I

E

E

T

I

K

Y

P

P

I

I

K
x
A

G

G

T

T

R

R

P

P

R

R

K

G

R

R

-

R

-

A

D

D

Q

G

A

T

E

L

D

D

R

R

-

D

-

L

-

D

Q

S

Q

R

I

I

E

E

I

L

-

D

L

M

E

R

H

T

S

D

P

H

K

K

D

E

R

L

A

S

E
|
E

N

H

L

L

M

M

I

L

V

V

D

D

L

L

L

A

R

R

N

N

D

D

I

L

S

A

K

R

T

I

C

C

K

T

K

P

G

G

S

S

V

R

K

Y

V

V

P

A

R

D

I

L

F

T

A

K

V

V

E

D

T

R

Y

Y

A

S

T

Y

V

V

H

M

H
|
H

L

L

V

V

S

S

T

R

I

V

T

V

G

G

W

E

L

L

A

R

D

H

D

D

R

L

H

D

A

A

L

L

D

H

L

A

L

Y

K

R

H

A

C

C

F

M

P

N

G

M

G

G

S
x
T

I

L

T

S

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

Q

V

L

I

I

E

A

E

D

L

A

E

E

P

G

N

Q

R

R

G

G

I

S

Y
|
Y

C

G

G

G

S

A

I

V

G
|
G

Y

Y

I

F

G

T

F

A

D

H

G

G

N

D

M

L

D

D

S

T

N
x
C

I

I

A

V

I

I

R
|
R

T

S

L

A

V

L

H

V

S

E

G

N

G

G

T

I

I

A

R

T

F

V

W

Q

A

A

G

G

G

A

G
|
G

I

I

V

V

N

L

D

D

S

S

E

V

V

P

D

Q

D

S

E

E

Y

A

Q

D

E
|
E

C

T

F

R

D

N

K
|
K

A

A

A

R

A

A

M

V

L

L

active site: Q259 (= Q212), E305 (= E259), A323 (≠ K275), E357 (= E303), H394 (= H340), T421 (≠ S367), Y445 (= Y391), R465 (= R411), G481 (= G427), E494 (= E440), K498 (= K444)
binding tryptophan: P287 (= P241), Y288 (≠ F242), M289 (≠ S243), G450 (= G396), C461 (≠ N407)

Sites not aligning to the query:

binding tryptophan: 34, 35, 36, 46

1i7qA Anthranilate synthase from s. Marcescens (see paper)
32% identity, 76% coverage: 104:449/458 of query aligns to 145:504/517 of 1i7qA

query
sites
1i7qA

G

G

Y

L

F

F

S

A

Y

Y

D

D

L

L

V

V

R

A

R

G

L

F

E

E

H

N

L

L

S

P

E

A

I

L

A

R

V

Q

D

D

A

-

E

Q

H

R

I

C

P

P

E

D

M

F

A

C

V

F

G

Y

I

L

Y

A

D

E

W

T

A

L

V

L

V

V

V

L

D

D

H

H

H

Q

-

R

-

G

-

S

-

A

-

R

C

L

R

Q

K

A

S

S

Y

V

L

F

V

S

G

E

Q

Q

N

A

C

S

D

E

M

A

Q

Q

K

R

W

L

L

Q

K

H

L

R

I

L

E

E

Q

Q

F

L

S

Q

V

A

-

E

L

L

P

Q

V

Q

E

P

R

Q

R

P

G

I

P

P

F

H

R

Q

I

K

V

L

G

E

D

N

I

M

R

Q

-

L

-

S

S

C

N

N

M

Q

D

S

K

D

A

E

F

E

Y

Y

T

G

R

A

A

V

F

V

D

S

R

E

I

L

K

Q

Q

E

Y

A

L

I

K

R

E

Q

G

G

D

E

C

I

Y

F

Q
|
Q

V

V

N

V

F

P

S

S

Q

R

R

R

F

F

V

S

A

L

D

P

C

C

E

P

G

A

D

-

P

P

W

L

T

G

A

P

Y

Y

K

Q

A

T

L

L

R

K

E

D

L

N

N

N

A

P

A

S

P

P

F

Y

S

M

C

F

Y

F

L

M

N

Q

L

D

P

D

E

D

V

F

Q

T

I

L

L

F

S

G

T

A

S

S

P

P

E
|
E

R

S

F

A

L

L

K

K

L

Y

T

D

D

A

G

G

-

N

-

R

L

Q

V

I

E

E

T

I

K

Y

P

P

I

I

K
x
A

G

G

T

T

R

R

P

P

R

R

K

G

R

R

-

R

-

A

D

D

Q

G

A

S

E

L

D

D

-

L

-

D

-

L

R

D

Q

S

Q

R

I

I

E

E

I

L

-

E

L

M

E

R

H

T

S

D

P

H

K

K

D

E

R

L

A

A

E
|
E

N

H

L

L

M

M

I

L

V

V

D

D

L

L

L

A

R

R

N

N

D

D

I

L

S

A

K

R

T

I

C

C

K

Q

K

A

G

G

S

S

V

R

K

Y

V

V

P

A

R

D

I

L

F

T

A

K

V

V

E

D

T

R

Y

Y

A

S

T

F

V

V

H

M

H
|
H

L

L

V

V

S

S

T

R

I

V

T

V

G

G

W

T

L

L

A

R

D

A

D

D

R

L

H

D

A

V

L

L

D

H

L

A

L

Y

K

Q

H

A

C

C

F

M

P

N

G

M

G

G

S
x
T

I

L

T

S

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

Q

V

L

I

I

E

A

L

L

E

R

P

S

N

T

R

R

G

G

I

S

Y
|
Y

C

G

G

G

S

R

I

V

G

G

Y

Y

I

F

G

T

F

A

D

V

G

R

N

N

M

L

D

D

S

T

N

C

I

I

A

V

I
|
I

R
|
R

T

S

L

A

V

Y

H

V

S

E

G

D

G

G

T

H

I

R

R

T

F

V

W

Q

A
|
A

G
|
G

G

A

G
|
G

I

V

V

V

N

Q

D

D

S

S

E

I

V

P

D

E

D

R

E

E

Y

A

Q

D

E
|
E

C

T

F

R

D

N

K
|
K

A

A

A

R

A

A

M

V

L

L

active site: Q260 (= Q212), E306 (= E259), A324 (≠ K275), E358 (= E303), H395 (= H340), T422 (≠ S367), Y446 (= Y391), R466 (= R411), G482 (= G427), E495 (= E440), K499 (= K444)
binding magnesium ion: E358 (= E303), E495 (= E440)
binding pyruvic acid: Y446 (= Y391), I465 (= I410), R466 (= R411), A479 (= A424), G480 (= G425), K499 (= K444)

1i7sA Anthranilate synthase from serratia marcescens in complex with its end product inhibitor l-tryptophan (see paper)
32% identity, 76% coverage: 104:449/458 of query aligns to 139:498/511 of 1i7sA

query
sites
1i7sA

G

G

Y

L

F

F

S

A

Y

Y

D

D

L

L

V

V

R

A

R

G

L

F

E

E

H

N

L

L

S

P

E

A

I

L

A

R

V

Q

D

D

A

-

E

Q

H

R

I

C

P

P

E

D

M

F

A

C

V

F

G

Y

I

L

Y

A

D

E

W

T

A

L

V

L

V

V

V

L

D

D

H

H

H

Q

-

R

-

G

-

S

-

A

-

R

C

L

R

Q

K

A

S

S

Y

V

L

F

V

S

G

E

Q

Q

N

A

C

S

D

E

M

A

Q

Q

K

R

W

L

L

Q

K

H

L

R

I

L

E

E

Q

Q

F

L

S

Q

V

A

-

E

L

L

P

Q

V

Q

E

P

R

Q

R

P

G

I

P

P

F

H

R

Q

I

K

V

L

G

E

D

N

I

M

R

Q

-

L

-

S

S

C

N

N

M

Q

D

S

K

D

A

E

F

E

Y

Y

T

G

R

A

A

V

F

V

D

S

R

E

I

L

K

Q

Q

E

Y

A

L

I

K

R

E

Q

G

G

D

E

C

I

Y

F

Q
|
Q

V

V

N

V

F

P

S

S

Q

R

R

R

F

F

V

S

A

L

D

P

C

C

E

P

G

A

D

-

P

P

W

L

T

G

A

P

Y

Y

K

Q

A

T

L

L

R

K

E

D

L

N

N

N

A

P

A

S

P
|
P

F
x
Y

S
x
M

C

F

Y

F

L

M

N

Q

L

D

P

D

E

D

V

F

Q

T

I

L

L

F

S

G

T

A

S

S

P

P

E
|
E

R

S

F

A

L

L

K

K

L

Y

T

D

D

A

G

G

-

N

-

R

L

Q

V

I

E

E

T

I

K

Y

P

P

I

I

K
x
A

G

G

T

T

R

R

P

P

R

R

K

G

R

R

-

R

-

A

D

D

Q

G

A

S

E

L

D

D

-

L

-

D

-

L

R

D

Q

S

Q

R

I

I

E

E

I

L

-

E

L

M

E

R

H

T

S

D

P

H

K

K

D

E

R

L

A

A

E
|
E

N

H

L

L

M

M

I

L

V

V

D

D

L

L

L

A

R

R

N

N

D

D

I

L

S

A

K

R

T

I

C

C

K

Q

K

A

G

G

S

S

V

R

K

Y

V

V

P

A

R

D

I

L

F

T

A

K

V

V

E

D

T

R

Y

Y

A

S

T

F

V

V

H

M

H
|
H

L

L

V

V

S

S

T

R

I

V

T

V

G

G

W

T

L

L

A

R

D

A

D

D

R

L

H

D

A

V

L

L

D

H

L

A

L

Y

K

Q

H

A

C

C

F

M

P

N

G

M

G

G

S
x
T

I

L

T

S

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

Q

V

L

I

I

E

A

L

L

E

R

P

S

N

T

R

R

G

G

I

S

Y
|
Y

C

G

G

G

S

R

I
x
V

G
|
G

Y

Y

I

F

G

T

F

A

D

V

G

R

N

N

M

L

D
|
D

S

T

N
x
C

I

I

A

V

I

I

R
|
R

T

S

L

A

V

Y

H

V

S

E

G

D

G

G

T

H

I

R

R

T

F

V

W

Q

A

A

G

G

G

A

G
|
G

I

V

V

V

N

Q

D

D

S

S

E

I

V

P

D

E

D

R

E

E

Y

A

Q

D

E
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E

C

T

F

R

D

N

K
|
K

A

A

A

R

A

A

M

V

L

L

active site: Q254 (= Q212), E300 (= E259), A318 (≠ K275), E352 (= E303), H389 (= H340), T416 (≠ S367), Y440 (= Y391), R460 (= R411), G476 (= G427), E489 (= E440), K493 (= K444)
binding tryptophan: P282 (= P241), Y283 (≠ F242), M284 (≠ S243), V444 (≠ I395), G445 (= G396), D454 (= D405), C456 (≠ N407)

Sites not aligning to the query:

binding tryptophan: 35, 36, 37

P00897 Anthranilate synthase component 1; AS; ASI; EC 4.1.3.27 from Serratia marcescens (see paper)
31% identity, 76% coverage: 104:449/458 of query aligns to 147:506/519 of P00897

query
sites
P00897

G

G

Y

L

F

F

S

A

Y

Y

D

D

L

L

V

V

R

A

R

G

L

F

E

E

H

N

L

L

S

P

E

A

I

V

A

R

V

Q

D

D

A

-

E

Q

H

R

I

C

P

P

E

D

M

F

A

C

V

F

G

Y

I

L

Y

A

D

E

W

T

A

L

V

L

V

V

V

L

D

D

H

H

H

Q

-

R

-

G

-

S

-

A

-

R

C

L

R

Q

K

A

S

S

Y

V

L

F

V

S

G

E

Q

Q

N

A

C

S

D

E

M

A

Q

Q

K

R

W

L

L

Q

K

H

L

R

I

L

E

E

Q

Q

F

L

S

Q

V

A

-

E

L

L

P

Q

V

Q

E

P

R

Q

R

P

G

I

P

P

F

H

R

Q

I

K

V

L

G

E

D

N

I

M

R

Q

-

L

-

S

S

C

N

N

M

Q

D

S

K

D

A

E

F

E

Y

Y

T

G

R

A

A

V

F

V

D

S

R

E

I

L

K

Q

Q

E

Y

A

L

I

K

R

E

Q

G

G

D

E

C

I

Y

F

Q

Q

V

V

N

V

F

P

S

S

Q

R

R

R

F

F

V

S

A

L

D

P

C

C

E

P

G

A

D

-

P

P

W

L

T

G

A

P

Y

Y

K

Q

A

T

L

L

R

K

E

D

L

N

N

N

A

P

A

S

P
|
P

F
x
Y

S
x
M

C

F

Y

F

L

M

N

Q

L

D

P

D

E

D

V

F

Q

T

I

L

L

F

S

G

T

A

S

S

P

P

E

E

R

S

F

A

L

L

K

K

L

Y

T

D

D

A

G

G

-

N

-

R

L

Q

V

I

E

E

T

I

K

Y

P

P

I

I

K

A

G

G

T

T

R

R

P

P

R

R

K

G

R

R

-

R

-

A

D

D

Q

G

A

S

E

L

D

D

-

L

-

D

-

L

R

D

Q

S

Q

R

I

I

E

E

I

L

-

E

L

M

E

R

H

T

S

D

P

H

K

K

D

E

R

L

A

A

E
|
E

N

H

L

L

M

M

I

L

V

V

D

D

L

L

L

A

R

R

N

N

D

D

I

L

S

A

K

R

T

I

C

C

K

Q

K

A

G

G

S

S

V

R

K

Y

V

V

P

A

R

D

I

L

F

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A

K

V

V

E

D

T

R

Y

Y

A

S

T

F

V

V

H

M

H

H

L

L

V

V

S

S

T

R

I

V

T

V

G

G

W

T

L

L

A

R

D

A

D

D

R

L

H

D

A

V

L

L

D

H

L

A

L

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K

Q

H

A

C

C

F

M

P

N

G

M

G

G

S

T

I

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T

S

G

G

A

A

P

P

K

K

I

V

R

R

A

A

M

M

E

Q

V

L

I

I

E

A

L

L

E

R

P

S

N

T

R

R

G

G

I

S

Y

Y

C

G

G

G

S

R

I

V

G

G

Y

Y

I

F

G

T

F

A

D

H

G

R

N

H

M

L

D

D

S

T

N

C

I

I

A

V

I

I

R

R

T

S

L

A

V

Y

H

V

S

E

G

D

G

G

T

H

I

R

R

T

F

V

W

Q

A

A

G

G

G

A

G

G

I

V

V

V

N

Q

D

D

S

S

E

I

V

R

D

R

E

E

Y

A

Q

D

E
|
E

C

T

F

R

D

N

K

K

A

A

A

R

A

A

M

V

L

L

PYM 290:292 (≠ PFS 241:243) binding L-tryptophan
E360 (= E303) binding Mg(2+)
E497 (= E440) binding Mg(2+)

Sites not aligning to the query:

39 binding L-tryptophan

8qc4A Salicylate synthase (see paper)
31% identity, 48% coverage: 210:429/458 of query aligns to 199:411/438 of 8qc4A

query
sites
8qc4A

C

C

Y

V

Q

E

V

V

N

P

F

F

S

A

Q

I

R

D

F

F

V

-

A

-

D

-

C

-

E

-

G

-

D

-

P

P

W

L

T

T

A

-

Y

Y

K

R

A

L

L

G

R

R

E

R

L

H

N

N

A

T

A

P

P

V

F

R

S

S

C

F

Y

L

L

L

N

Q

L

L

P

G

E

G

V

I

Q

R

I

A

L

L

S

G

T

Y

S

S

P

P

E

E

R

L

F

V

L

T

K

A

L

V

-

R

T

A

D

D

G

G

L

V

V

V

E

I

T

T

K

E

P

P

I

L

K
x
A

G

G

T

T

R

R

P

A

R

L

K

G

R

R

D

G

Q

P

A

A

E

I

D

D

R

R

Q

L

Q

A

I

R

E

D

I

D

L

L

E

E

H

S

S

N

P

S

K

K

D

E

R

I

A

V

E

E

N

H

L

A

M

I

I

S

V

V

D

R

L

S

R

L

N

E

D

E

I

I

S

T

K

D

T

I

C

A

K

E

K

P

G

G

S

S

V

A

K

A

V

V

P

I

R

D

I

F

F

M

A

T

V

V

E

R

T

E

Y

R

A

G

T

S

V

V

H

Q

H
|
H

L

L

V

G

S

S

T

T

I

I

T

R

G

A

W

R

L

L

A

D

D

P

D

S

R

S

H

D

A

R

L

M

D

A

L

A

L

L

K

E

H

A

C

L

F

F

P

P

G

A

G

V

S
x
T

I

A

T

S

G

G

A

I

P

P

K

K

I

A

R

A

A

G

M

V

E

E

V

A

I

I

E

F

E

R

L

L

E

D

P

E

N

C

R

P

R

R

G

G

I

L

Y
|
Y

C

S

G

G

S

A

I

V

G

V

Y

M

I

L

G

S

F

A

D

D

G

G

N

G

M

L

D

D

S

A

N

A

I

L

A

T

I
x
L

R
|
R

T

A

L

A

V

Y

H

Q

S

V

G

G

T

R

I

T

R

W

F

L

W

R

A
|
A

G
|
G

G

A

G

G

I

I

V

I

binding 5-(3-carboxyphenyl)furan-2-carboxylic acid: A257 (≠ K275), H322 (= H340), T349 (≠ S367), Y373 (= Y391), L392 (≠ I410), R393 (= R411), A406 (= A424), G407 (= G425)

Sites not aligning to the query:

binding 5-(3-carboxyphenyl)furan-2-carboxylic acid: 193, 195, 426

Query Sequence

>WP_020561754.1 NCBI__GCF_000372865.1:WP_020561754.1
MSPLNQFMSPLPYFADSADLFSAIAGMPWSVFLDSGYPYSQQGRYDIIAADPVCILVTHG
DETVITRGGKSVISKDDPFDLVKQQLLPAHCQGDTLPFKGGAIGYFSYDLVRRLEHLSEI
AVDAEHIPEMAVGIYDWAVVVDHHCRKSYLVGQNCDMQKWLKLIEQFSVLPVEERRGPFR
IVGDIRSNMDKAFYTRAFDRIKQYLKEGDCYQVNFSQRFVADCEGDPWTAYKALRELNAA
PFSCYLNLPEVQILSTSPERFLKLTDGLVETKPIKGTRPRKRDQAEDRQQIEILEHSPKD
RAENLMIVDLLRNDISKTCKKGSVKVPRIFAVETYATVHHLVSTITGWLADDRHALDLLK
HCFPGGSITGAPKIRAMEVIEELEPNRRGIYCGSIGYIGFDGNMDSNIAIRTLVHSGGTI
RFWAGGGIVNDSEVDDEYQECFDKAAAMLDLLQRFRVG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory