SitesBLAST
Comparing WP_020562014.1 NCBI__GCF_000372865.1:WP_020562014.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5nccA Structure of fatty acid photodecarboxylase in complex with fad and palmitic acid (see paper)
37% identity, 98% coverage: 7:529/531 of query aligns to 25:572/578 of 5nccA
- active site: R347 (≠ A319), L420 (≠ A381), I421 (≠ Y382), S507 (≠ T466), A509 (≠ H468), G552 (= G509), Q553 (≠ N510)
- binding flavin-adenine dinucleotide: G30 (= G12), G32 (= G14), T33 (≠ S15), A34 (= A16), L53 (= L35), E54 (= E36), A55 (= A37), F74 (≠ Q56), W80 (= W62), A98 (≠ P80), G100 (= G82), G105 (= G87), S106 (= S88), N110 (= N92), A111 (≠ Y93), T112 (≠ M94), L113 (≠ I95), V238 (= V219), A278 (= A253), H282 (≠ R257), L286 (= L261), N508 (≠ W467), Q553 (≠ N510), T554 (= T511), G555 (≠ N512), V558 (= V515)
A0A248QE08 Fatty acid photodecarboxylase, chloroplastic; CvFAP; EC 4.1.1.106 from Chlorella variabilis (Green alga) (see paper)
36% identity, 98% coverage: 7:529/531 of query aligns to 85:639/654 of A0A248QE08
- TA 93:94 (≠ SA 15:16) binding FAD
- E114 (= E36) binding FAD
- L162 (≠ G84) binding FAD
- S166 (= S88) binding FAD
- NATL 170:173 (≠ NYMI 92:95) binding FAD
- V298 (= V219) binding FAD
- C432 (≠ L337) binding hexadecanoate
- R451 (≠ F357) binding hexadecanoate
- Y466 (≠ P372) binding hexadecanoate
- Q486 (≠ T380) binding hexadecanoate
- G622 (≠ N512) binding FAD
6yrvAAA structure of fap after illumination at 100k (see paper)
36% identity, 98% coverage: 7:529/531 of query aligns to 9:563/573 of 6yrvAAA
- binding carbon dioxide: R375 (≠ F357), N499 (≠ W467)
- binding flavin-adenine dinucleotide: G14 (= G12), G16 (= G14), T17 (≠ S15), A18 (= A16), L37 (= L35), E38 (= E36), A39 (= A37), F58 (≠ Q56), W64 (= W62), A82 (≠ P80), G89 (= G87), S90 (= S88), N94 (= N92), A95 (≠ Y93), T96 (≠ M94), L97 (≠ I95), M191 (≠ T188), V222 (= V219), C264 (≠ S252), A265 (= A253), G266 (= G254), H269 (≠ R257), N499 (≠ W467), A534 (= A500), Q544 (≠ N510), T545 (= T511), G546 (≠ N512)
- binding heptadecane: V377 (≠ F359), G379 (≠ N361), M380 (≠ L362), G386 (≠ E368), T389 (= T371), Y390 (≠ P372), F393 (≠ H375), T408 (≠ A378), Q410 (≠ T380)
8b7sA Crystal structure of the chloramphenicol-inactivating oxidoreductase from novosphingobium sp (see paper)
37% identity, 98% coverage: 7:529/531 of query aligns to 6:453/458 of 8b7sA
- binding flavin-adenine dinucleotide: G11 (= G12), G13 (= G14), S14 (= S15), A15 (= A16), E35 (= E36), A36 (= A37), W47 (= W62), P65 (= P80), G67 (= G82), V180 (= V219), A214 (= A253), G215 (= G254), A218 (≠ R257), T270 (= T327), Y391 (≠ W467), A424 (= A500), I435 (≠ T511), N436 (= N512)
5oc1A Crystal structure of aryl-alcohol oxidase from pleurotus eryngii in complex with p-anisic acid (see paper)
33% identity, 98% coverage: 7:527/531 of query aligns to 3:562/565 of 5oc1A
- active site: V339 (≠ A321), N413 (≠ A381), A414 (≠ Y382), I499 (≠ T466), H501 (= H468), A544 (≠ G509), H545 (≠ N510)
- binding 4-methoxybenzoic acid: Y91 (= Y93), I356 (≠ E338), I390 (≠ L358), F396 (≠ E365), T412 (= T380), I499 (≠ T466), H501 (= H468), H545 (≠ N510)
- binding flavin-adenine dinucleotide: G8 (= G12), G10 (= G14), N11 (≠ S15), A12 (= A16), E32 (= E36), A33 (= A37), W60 (= W62), P78 (= P80), G80 (= G82), G85 (= G87), S86 (= S88), H90 (≠ N92), Y91 (= Y93), V93 (≠ I95), V230 (= V219), S270 (= S252), A271 (= A253), G272 (= G254), F500 (≠ W467), H545 (≠ N510), T546 (= T511), Q547 (≠ N512), I550 (≠ V515)
E4QP00 5-(hydroxymethyl)furfural oxidase; 5-hydroxymethylfurfural oxidase; HMFO; Thiol oxidase; EC 1.1.3.47; EC 1.8.3.- from Methylovorus sp. (strain MP688) (see paper)
38% identity, 99% coverage: 1:526/531 of query aligns to 1:527/531 of E4QP00
- V101 (≠ I91) mutation to H: Abolishes activity.
- M103 (≠ Y93) mutation to A: 16-fold reduction in catalytic efficiency on vanillyl alcohol.
- V367 (≠ T380) mutation to K: 1.6-fold reduction in catalytic efficiency on vanillyl alcohol. Shows significantly improved activity on the aldehyde 5-formyl-2-furancarboxylate, which results in a better 5-hydroxymethylfurfural to 2,5-furandicarboxylate conversion.; mutation to R: 1.4-fold reduction in catalytic efficiency on vanillyl alcohol. Shows significantly improved activity on the aldehyde 5-formyl-2-furancarboxylate, which results in a better 5-hydroxymethylfurfural to 2,5-furandicarboxylate conversion. Displays a catalytic efficiency toward 5-formyl-2-furancarboxylate that is over 1000-fold higher than that for wild-type; when associated with F-466.
- W369 (≠ Y382) mutation to A: 7.5-fold reduction in catalytic efficiency on vanillyl alcohol.
- V465 (≠ T466) mutation to A: 18-fold reduction in catalytic efficiency on vanillyl alcohol.
- W466 (= W467) mutation to A: 39-fold reduction in catalytic efficiency on vanillyl alcohol. In contrast to wild-type, is active on secondary alcohols, such as (S)-1-phenylethanol, and is strictly enantionselective as this mutant has no activity on (R)-1-phenylethanol. Shows increased activity on the aldehyde 5-formyl-2-furancarboxylate.; mutation to F: 3.4-fold reduction in catalytic efficiency on vanillyl alcohol. In contrast to wild-type, is active on secondary alcohols, such as (S)-1-phenylethanol, and is strictly enantionselective as this mutant has no activity on (R)-1-phenylethanol. Shows increased activity on the aldehyde 5-formyl-2-furancarboxylate. Displays a catalytic efficiency toward 5-formyl-2-furancarboxylate that is over 1000-fold higher than that for wild-type; when associated with R-367.
- H467 (= H468) mutation to A: Abolishes activity.
- N511 (= N510) mutation to A: 53-fold reduction in catalytic efficiency on vanillyl alcohol.
3fimB Crystal structure of aryl-alcohol-oxidase from pleurotus eryingii (see paper)
33% identity, 98% coverage: 7:527/531 of query aligns to 3:562/565 of 3fimB
- active site: V339 (≠ A321), N413 (≠ A381), A414 (≠ Y382), I499 (≠ T466), H501 (= H468), A544 (≠ G509), H545 (≠ N510)
- binding flavin-adenine dinucleotide: G8 (= G12), N11 (≠ S15), A12 (= A16), E32 (= E36), A33 (= A37), W60 (= W62), P78 (= P80), G80 (= G82), G85 (= G87), S86 (= S88), H90 (≠ N92), Y91 (= Y93), V93 (≠ I95), V230 (= V219), S270 (= S252), A271 (= A253), F500 (≠ W467), H501 (= H468), H545 (≠ N510), T546 (= T511), Q547 (≠ N512), I550 (≠ V515)
4udqA Crystal structure of 5-hydroxymethylfurfural oxidase (hmfo) in the reduced state
38% identity, 98% coverage: 5:526/531 of query aligns to 1:523/525 of 4udqA
- active site: L331 (≠ A339), F364 (≠ A381), W365 (≠ Y382), V461 (≠ T466), H463 (= H468), A506 (≠ G509), N507 (= N510)
- binding flavin-adenine dinucleotide: G8 (= G12), G10 (= G14), T11 (≠ S15), A12 (= A16), E32 (= E36), A33 (= A37), W64 (= W62), G88 (= G82), G93 (= G87), G94 (≠ S88), N98 (= N92), M99 (≠ Y93), V101 (≠ I95), V229 (= V219), T261 (≠ S252), A262 (= A253), W462 (= W467), H463 (= H468), A497 (= A500), N507 (= N510), T508 (= T511), N509 (= N512), T512 (≠ V515)
4mjwA Crystal structure of choline oxidase in complex with the reaction product glycine betaine (see paper)
36% identity, 98% coverage: 7:528/531 of query aligns to 15:528/532 of 4mjwA
- active site: I333 (≠ A339), P377 (≠ A381), N378 (≠ Y382), V464 (≠ T466), H466 (= H468), V509 (≠ G509), N510 (= N510)
- binding flavin-adenine dinucleotide: G20 (= G12), G22 (= G14), S23 (= S15), E44 (= E36), A45 (= A37), W71 (= W62), R89 (≠ Q81), A90 (≠ G82), G95 (= G87), C96 (≠ S88), H99 (≠ I91), N100 (= N92), S101 (≠ Y93), I103 (= I95), R231 (≠ H218), A232 (≠ V219), T269 (≠ A253), G270 (= G254), D273 (≠ R257), Y465 (≠ W467), H466 (= H468), A500 (= A500), N510 (= N510), P511 (≠ T511), N512 (= N512), V515 (= V515)
2jbvA Crystal structure of choline oxidase reveals insights into the catalytic mechanism (see paper)
36% identity, 98% coverage: 7:527/531 of query aligns to 15:527/527 of 2jbvA
- active site: I333 (≠ A339), P377 (≠ A381), N378 (≠ Y382), V464 (≠ T466), H466 (= H468), V509 (≠ G509), N510 (= N510)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-5-[(4aS,10aR)-7,8-dimethyl-2,4-dioxo-1,3,4,4a,5,10a-hexahydrobenzo[g]pteridin-10(2H)-yl]-2,3,4-trihydroxypentyl dihydrogen diphosphate: G22 (= G14), S23 (= S15), E44 (= E36), A45 (= A37), W71 (= W62), A90 (≠ G82), G95 (= G87), C96 (≠ S88), H99 (≠ I91), N100 (= N92), S101 (≠ Y93), I103 (= I95), R231 (≠ H218), A232 (≠ V219), T269 (≠ A253), G270 (= G254), D273 (≠ R257), V464 (≠ T466), Y465 (≠ W467), H466 (= H468), D499 (= D499), A500 (= A500), N510 (= N510), P511 (≠ T511), N512 (= N512), V515 (= V515)
3ljpA Crystal structure of choline oxidase v464a mutant (see paper)
36% identity, 98% coverage: 7:528/531 of query aligns to 15:528/530 of 3ljpA
- active site: I333 (≠ A339), P377 (≠ A381), N378 (≠ Y382), A464 (≠ T466), H466 (= H468), V509 (≠ G509), N510 (= N510)
- binding dihydroflavine-adenine dinucleotide: G22 (= G14), S23 (= S15), E44 (= E36), A45 (= A37), W71 (= W62), R89 (≠ Q81), A90 (≠ G82), G95 (= G87), C96 (≠ S88), H99 (≠ I91), N100 (= N92), S101 (≠ Y93), I103 (= I95), A232 (≠ V219), T269 (≠ A253), D273 (≠ R257), Y465 (≠ W467), H466 (= H468), D499 (= D499), A500 (= A500), N510 (= N510), P511 (≠ T511), N512 (= N512), V515 (= V515)
3t37A Crystal structure of pyridoxine 4-oxidase from mesorbium loti
36% identity, 97% coverage: 7:523/531 of query aligns to 3:501/509 of 3t37A
- active site: F360 (vs. gap), G361 (vs. gap), H444 (≠ T466), H446 (= H468), G487 (= G509), P488 (≠ N510)
- binding flavin-adenine dinucleotide: G8 (= G12), G10 (= G14), S11 (= S15), A12 (= A16), E32 (= E36), A33 (= A37), W58 (= W62), R77 (≠ Q81), G78 (= G82), R79 (= R83), G83 (= G87), S84 (= S88), H88 (≠ N92), A89 (≠ Y93), G91 (≠ I95), R217 (≠ H218), V218 (= V219), A251 (= A253), E255 (≠ R257), H445 (≠ W467), A478 (= A500), P488 (≠ N510), I489 (≠ T511), H490 (≠ N512)
4ha6A Crystal structure of pyridoxine 4-oxidase - pyridoxamine complex (see paper)
36% identity, 97% coverage: 7:523/531 of query aligns to 3:501/508 of 4ha6A
- active site: F360 (vs. gap), G361 (vs. gap), H444 (≠ T466), H446 (= H468), G487 (= G509), P488 (≠ N510)
- binding flavin-adenine dinucleotide: G8 (= G12), G10 (= G14), S11 (= S15), A12 (= A16), E32 (= E36), A33 (= A37), W58 (= W62), R77 (≠ Q81), G78 (= G82), G83 (= G87), S84 (= S88), L87 (≠ I91), H88 (≠ N92), A89 (≠ Y93), M90 (= M94), G91 (≠ I95), V218 (= V219), A251 (= A253), G252 (= G254), E255 (≠ R257), H445 (≠ W467), A478 (= A500), P488 (≠ N510), I489 (≠ T511), H490 (≠ N512)
- binding 4-(aminomethyl)-5-(hydroxymethyl)-2-methylpyridin-3-ol: A89 (≠ Y93), S314 (≠ N335), H444 (≠ T466), H446 (= H468)
3q9tA Crystal structure analysis of formate oxidase (see paper)
32% identity, 98% coverage: 7:527/531 of query aligns to 8:570/577 of 3q9tA
- active site: A335 (= A319), D422 (≠ N384), A508 (≠ T466), H510 (= H468), C552 (≠ G509), R553 (≠ N510)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl(2R,3S,4S)-5-(8-formyl-7-methyl-2,4-dioxo-3,4-dihydrobenzo[g]pteridin-10(2H)-yl)-2,3,4-trihydroxypentyl dihydrogendiphosphate: G15 (= G14), T16 (≠ S15), E37 (= E36), A38 (= A37), W65 (= W62), T85 (≠ G82), R86 (= R83), G87 (= G84), G92 (= G87), S93 (= S88), N97 (= N92), Y98 (= Y93), F99 (≠ M94), T100 (≠ I95), S229 (≠ V219), S265 (= S252), Q266 (≠ A253), E270 (≠ R257), F509 (≠ W467), D542 (= D499), A543 (= A500), R553 (≠ N510), I554 (≠ T511), Q555 (≠ N512), V558 (= V515)
5zu2A Effect of mutation (r554a) on fad modification in aspergillus oryzae rib40formate oxidase (see paper)
32% identity, 98% coverage: 7:527/531 of query aligns to 8:570/577 of 5zu2A
- binding flavin-adenine dinucleotide: G15 (= G14), T16 (≠ S15), E37 (= E36), A38 (= A37), W65 (= W62), T85 (≠ G82), R86 (= R83), G87 (= G84), G92 (= G87), S93 (= S88), N97 (= N92), Y98 (= Y93), T100 (≠ I95), S229 (≠ V219), S265 (= S252), Q266 (≠ A253), E270 (≠ R257), F509 (≠ W467), D542 (= D499), A543 (= A500), A553 (≠ N510), I554 (≠ T511), Q555 (≠ N512), V558 (= V515)
4h7uA Crystal structure of pyranose dehydrogenase from agaricus meleagris, wildtype (see paper)
32% identity, 98% coverage: 7:526/531 of query aligns to 17:572/577 of 4h7uA
- active site: A343 (= A319), V426 (= V383), Y510 (≠ T466), H512 (= H468), A555 (≠ G509), H556 (≠ N510)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-2,3,4-trihydroxy-5-[(4aR)-4a-hydroxy-7,8-dimethyl-2,4-dioxo-3,4,4a,5-tetrahydrobenzo[g]pteridin-10(2H)-yl]pentyl dihydrogen diphosphate (non-preferred name): G22 (= G12), G24 (= G14), T25 (≠ S15), A26 (= A16), E46 (= E36), A47 (= A37), W74 (= W62), G99 (= G87), C100 (≠ S88), H103 (≠ I91), N104 (= N92), G105 (≠ Y93), V107 (≠ I95), L242 (≠ H218), V243 (= V219), G282 (≠ A253), G283 (= G254), A286 (≠ R257), H512 (= H468), A546 (= A500), H556 (≠ N510), T557 (= T511), Q558 (≠ N512), V561 (= V515)
Q3L245 Pyranose dehydrogenase 1; PDH 1; Pyranose:quinone oxidoreductase 1; EC 1.1.99.29 from Leucoagaricus meleagris (Western flat-topped agaric) (Agaricus meleagris) (see 2 papers)
32% identity, 98% coverage: 7:526/531 of query aligns to 42:597/602 of Q3L245
- N100 (≠ G63) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- H128 (≠ I91) modified: Tele-8alpha-FAD histidine
- N344 (= N290) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- H537 (= H468) active site, Proton acceptor
- H581 (≠ N510) active site
Sites not aligning to the query:
7vzsA Fad-dpendent glucose dehydrogenase complexed with an inhibitor at ph7.56
32% identity, 98% coverage: 7:527/531 of query aligns to 5:563/566 of 7vzsA
- binding D-glucal: Y6 (= Y8), L22 (= L24), N25 (≠ D27), Y51 (≠ F52), I349 (≠ A319), Q356 (≠ A326), E411 (vs. gap), E444 (≠ A413), W445 (≠ D414), K448 (≠ C417), R499 (≠ S464), N501 (≠ T466), H546 (≠ N510), K563 (= K527)
- binding flavin-adenine dinucleotide: G10 (= G12), G12 (= G14), T13 (≠ S15), S14 (≠ A16), E34 (= E36), A35 (= A37), Y51 (≠ F52), F55 (≠ Q56), W61 (= W62), R79 (≠ P80), G81 (= G82), G86 (= G87), T87 (≠ S88), N91 (= N92), G92 (≠ Y93), M93 (= M94), A94 (≠ I95), T232 (≠ H218), A233 (≠ V219), A273 (= A253), G274 (= G254), R277 (= R257), F502 (≠ W467), A536 (= A500), H546 (≠ N510), L547 (≠ T511), V548 (≠ N512), L551 (≠ V515)
Sites not aligning to the query:
4ynuA Crystal structure of aspergillus flavus fadgdh in complex with d- glucono-1,5-lactone (see paper)
32% identity, 98% coverage: 7:527/531 of query aligns to 5:563/569 of 4ynuA
- active site: V341 (≠ A311), F412 (vs. gap), W413 (≠ Y382), N501 (≠ T466), H503 (= H468), G545 (= G509), H546 (≠ N510)
- binding flavin-adenine dinucleotide: G12 (= G14), T13 (≠ S15), S14 (≠ A16), E34 (= E36), A35 (= A37), Y51 (≠ F52), F55 (≠ Q56), W61 (= W62), R79 (≠ P80), G81 (= G82), G86 (= G87), T87 (≠ S88), N91 (= N92), G92 (≠ Y93), T232 (≠ H218), A233 (≠ V219), A273 (= A253), G274 (= G254), R277 (= R257), F502 (≠ W467), A536 (= A500), H546 (≠ N510), L547 (≠ T511), V548 (≠ N512), L551 (≠ V515)
- binding D-glucono-1,5-lactone: Y51 (≠ F52), E411 (vs. gap), A496 (≠ R461), N497 (≠ T462), R499 (≠ S464), R499 (≠ S464), N501 (≠ T466), H503 (= H468), H546 (≠ N510)
4yntA Crystal structure of aspergillus flavus fad glucose dehydrogenase (see paper)
32% identity, 98% coverage: 7:527/531 of query aligns to 6:564/570 of 4yntA
- active site: V342 (≠ A311), F413 (vs. gap), W414 (≠ Y382), N502 (≠ T466), H504 (= H468), G546 (= G509), H547 (≠ N510)
- binding dihydroflavine-adenine dinucleotide: G13 (= G14), T14 (≠ S15), S15 (≠ A16), E35 (= E36), A36 (= A37), F56 (≠ Q56), W62 (= W62), R80 (≠ P80), G82 (= G82), G87 (= G87), T88 (≠ S88), N92 (= N92), G93 (≠ Y93), M94 (= M94), A95 (≠ I95), A234 (≠ V219), A274 (= A253), R278 (= R257), F503 (≠ W467), A537 (= A500), H547 (≠ N510), L548 (≠ T511), V549 (≠ N512), L552 (≠ V515)
Query Sequence
>WP_020562014.1 NCBI__GCF_000372865.1:WP_020562014.1
MPRPIVDYIIVGAGSAGCVLAHRLSEDPACRVLLLEAGGEGDAAAVRTPGLFSRLQDSAY
DWGDRTLPQQAMQGRRIYLPQGRGIGGSSSINYMIYMRGNRRDYDRWVDLGNAGWSYEEV
LPYFIKSETNLAFADRYHGRTGPLKVSSHPQLSPVTRRYLEAAQEVGLPYNPDFNGERQE
GCGPLQRTIADGIRCSAAQAYLQPVRHRPNLSVIDHAHVTSLLFRNDRITGVQYLRYGVV
EQAHAAVETILSAGALRSPQLLMLSGLGPARELERLGIEVRADMPEVGRNLQDHVHARVR
CELTRPLTFAALSGEEKAAAAALYDATGTGPLSSNFLEAGCFVDSRSEAGYPDLQLFLFG
NLTPEYPEAATPDRHGIALTAYVNRPRSRGRVTLSSADPLDRPRIDPNYFSDADDLCCLV
AGVRWNLKILYGKAFDDVRGNELSPGAAIRNDAGLEDFVLRTASTTWHPTSTCRMGADAG
AVVDPFLRVNGVSGLRVIDASVMPAIVSGNTNAPVIMIAEKAADLIKNRGA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory