SitesBLAST

5e25A Crystal structure of branched-chain aminotransferase from thermophilic archaea geoglobus acetivorans complexed with alpha-ketoglutarate (see paper)
32% identity, 90% coverage: 1:249/276 of query aligns to 4:264/290 of 5e25A

query
sites
5e25A

M

L

I

V

L

Y

I

M

N

N

G

G

E

E

Y

F

-

V

-

P

-

E

-

S

R

Q

D

A

H

K

I

V

E

S

I

V

S

F

D

D

R

H

G

G

F

F

Q

L

Y

Y

G

G

D

D

G

G

L

V

F
|
F

E

E

T
x
G

I

I

E

R

V

A

H

Y

N

N

G

G

R

K

P

V

V

F

F

K

L

L

N

Y

R

E

H

H

L

I

H

D

R
|
R

L

L

Q

Y

T

D

G

-

C

C

A

A

R

R

-

V

-

I

-

D

L

L

Q

K

I

I

P

P

C

L

P

S

D

K

P

E

E

E

L

F

I

A

R

E

S

A

E

I

A

L

A

E

V

T

L

L

C

R

K

R

N

N

A

N

P

L

H

R

-

D

A

A

V
x
Y

L

I

K

R

L

P

I

I

I

V

T

T

R

R

G

G

S

A

G

G

G

D

R

L

G

G

Y

L

R

D

P

P

P

R

E

K

A

C

T

P

R

S

P

P

T

N

R

V

V

I

L

I

S

I

L

T

H

K

P

P

C

W

P

G

D

K

Y

L

P

Y

G

G

R

D

Y

L

R

Y

E

E

Q

K

G

G

V

L

-

K

A

A

V

I

R

T

I

V

C

A

Q

I

T

R

R

R

L

N

G

A

L

I

N

D

P

S

A

L

L

P

A

P

G

N

I

I

K
|
K

H

S

L

L

N

N

R
x
Y

L

L

E

N

Q

N

V

I

L

L

A

A

R

K

A

I

E

E

W

A

D

N

D

A

P

K

E

G

I

G

Q

D

E

E

G

A

L

I

M

F

L

L

D

D

I

H

N

N

G

G

H

Y

V

I

I

S

E
|
E

G

G

T

S

M
x
G

T
x
D

N

N

L

I

F

F

Y

I

F

V

S

K

N

N

R

G

S

T

A

I

Y

T

T

T

P

P

A

P

L

-

T

T

Q

L

C

N

G

N

V
x
L

D

K

G
|
G

I
|
I

I
x
T

R

R

R

Q

I

V

L

V

M

I

D

E

L

L

L

I

S

N

K

E

R

L

R

E

I

I

T

P

V

F

R

R

E

E

T

A

A

N

P

I

T

G

V

L

D

F

E

D

L

L

S

Y

A

S

A

A

D

D

E

E

V

I

F

F

V

V

C

T

N
x
G

S
x
T

I
x
A

I

A

G

E

I

I

W

A

P

P

V

V

-

T

-

Y

-

I

-

D

-

G

R

R

A

T

I

V

G

G

N

N

active site: F33 (= F26), G35 (≠ T28), K151 (= K140), E184 (= E173), L207 (≠ V197)
binding 2-oxoglutaric acid: Y88 (≠ V78), K151 (= K140), T247 (≠ S237), A248 (≠ I238)
binding pyridoxal-5'-phosphate: R52 (= R45), K151 (= K140), Y155 (≠ R144), E184 (= E173), G187 (≠ M176), D188 (≠ T177), L207 (≠ V197), G209 (= G199), I210 (= I200), T211 (≠ I201), G246 (≠ N236), T247 (≠ S237)

5mr0D Thermophilic archaeal branched-chain amino acid transaminases from geoglobus acetivorans and archaeoglobus fulgidus: biochemical and structural characterisation (see paper)
31% identity, 89% coverage: 2:247/276 of query aligns to 4:256/290 of 5mr0D

query
sites
5mr0D

I

V

L

Y

I

M

N

D

G

G

E

E

Y

F

-

V

-

P

-

E

-

N

R

E

D

A

H

K

I

V

E

S

I

I

S

F

D

D

R

H

G

G

F

F

Q

L

Y

Y

G

G

D

D

G

G

L

V

F
|
F

E

E

T
x
G

I

I

E

R

V

A

H

Y

N

N

G

G

R

R

P

V

V

F

F

R

L

L

N

K

R

E

H

H

L

I

H

D

R
|
R

L

L

-

Y

-

D

Q

S

T

A

G

K

C

A

A

I

R

D

L

L

Q

E

I

I

P

P

C

I

P

T

D

K

P

E

E

E

L

F

I

M

R

E

S

I

E

I

A

L

A

E

V

T

L

L

C

R

K

K

-

N

N

N

A

L

P

R

H

D

A

A

V

Y

L

I

K

R

L

P

I

I

I

V

T

T

R

R

G

G

S

I

G

G

G

D

R

L

G
|
G

Y
x
L

R

D

P

P

P

R

E

K

A

C

T

Q

R

N

P

P

T

S

R

I

V

I

L

V

S

I

L

T

H

K

P

P

C

W

P

G

D

K

Y

L

P

Y

G

G

R

D

Y

L

R

Y

E

E

Q

K

G

G

V

L

-

T

A

A

V

I

R

T

I

V

C

A

Q

V

T

R

R

R

L

N

G

S

L

F

N

D

P

A

A

L

L

P

A

P

G

N

I

I

K
|
K

H

S

L

L

N

N

R
x
Y

L

L

E

N

Q

N

V

I

L

L

A

A

R

K

A

I

E

E

W

A

D

N

D

A

P

K

E

G

I

G

Q

D

E

E

G

A

L

I

M

F

L

L

D

D

I

R

N

N

G

G

H

Y

V

V

I

S

E
|
E

G

G

T

S

M
x
G

T
x
D

N

N

L

I

F

F

Y

V

F

V

S

K

N

N

R

G

S

A

A

I

Y

T

T

T

P

P

A

P

L

-

T

T

Q

I

C

N

G

N

V
x
L

D

R

G

G

I
|
I

I
x
T

R

R

R

E

I

A

L

V

M

I

D

E

L

I

S

N

K

R

R

L

R

G

I

I

T

P

V

F

R

K

E

E

T

T

A

N

P

I

T

G

V

L

D

Y

E

D

L

L

S

Y

A

T

A

A

D

D

E

E

V

V

F

F

V

V

C

T

N
x
G

S
x
T

I

A

G

E

I

I

W

A

P

P

V

I

R

V

A

V

I

I

active site: F32 (= F26), G34 (≠ T28), K150 (= K140), E183 (= E173), L206 (≠ V197)
binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: R51 (= R45), G100 (= G91), L101 (≠ Y92), K150 (= K140), Y154 (≠ R144), E183 (= E173), G186 (≠ M176), D187 (≠ T177), L206 (≠ V197), I209 (= I200), T210 (≠ I201), G245 (≠ N236), T246 (≠ S237)

1iyeA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
29% identity, 95% coverage: 2:262/276 of query aligns to 5:282/304 of 1iyeA

query
sites
1iyeA

I

I

L

W

I

F

N

N

G

G

E

E

-

M

-

V

-

R

Y

W

R

E

D

D

-

A

H

K

I

V

E

H

I

V

S

M

D

S

R

H

G

A

F

L

Q

H

Y

Y

G

G

D

T

G

S

L

V

F
|
F

E

E

T
x
G

I

I

-

R

-

C

-

Y

E

D

V

S

H

H

N

K

G

G

R

P

P

V

V

V

F

F

L

R

N

H

R

R

-

E

H

H

L

M

H

Q

R
|
R

L

L

Q

H

T

D

G

S

C

A

A

K

R

I

L

Y

Q

R

I

F

P

P

C

V

P

S

D

Q

P

S

-

I

-

D

E

E

L

L

I

M

R

E

S

A

E

C

A

R

A

D

V

V

L

I

C

R

K

K

N

N

A

N

-

L

P

T

H

S

A

A

V
x
Y

L

I

K

R

L

P

I

L

I

I

T

F

R

V

G

G

S

D

G

V

G

G

R

M

G

G

Y

V

R

N

P

P

E

A

A

G

T

Y

R

S

P

T

T

D

R

V

V

I

L

I

S

A

L

A

H

F

P

P

C

W

P

G

D

A

Y
|
Y

P

L

G

G

-

A

R

E

Y

A

R

L

E

E

Q

Q

G

G

V

I

A

D

V

A

R

M

I

V

C

S

Q

S

-

W

T

N

R

R

L

A

G

A

L

P

N

N

P

T

A

I

L

P

A

T

G

A

I

A

K
|
K

H
x
A

L

G

-

G

N

N

R
x
Y

L

L

E

S

Q

S

V

L

L

L

A

V

R

G

A

S

E

E

W

A

D

R

P

H

E

G

I

Y

Q

Q

E

E

G

G

L

I

M

A

L

L

D

D

I

V

N

N

G

G

H

Y

V

I

I

S

E
|
E

G

G

T

A

M
x
G

T
x
E

N
|
N

L

L

F

F

Y

E

F

V

S

K

N

D

R

G

S

V

A

L

Y

F

T

T

P

P

A

P

L

F

T

T

Q

S

C

S

G

A

V
x
L

D

P

G
|
G

I
|
I

I
x
T

R

R

R

D

I

A

L

I

M

I

D

K

L

L

L

A

S

K

K

E

R

L

R

G

I

I

T

E

V

V

R

R

E

E

T

Q

A

V

P

L

T

S

V

R

D

E

E

S

L

L

S

Y

A

L

A

A

D

D

E

E

V

V

F

F

V

M

C

S

N
x
G

S
x
T

I
x
A

I

A

G

E

I

I

W

T

P

P

V

V

R

R

A

S

I

V

G

D

N

G

V

I

R

Q

Y

V

A

G

-

E

-

G

-

R

V

C

G

G

P

P

F

V

T

T

R

K

Q

R

C

I

Q

Q

active site: F33 (= F26), G35 (≠ T28), K156 (= K140), A157 (≠ H141), E190 (= E173), L214 (≠ V197)
binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: R56 (= R45), Y92 (≠ V78), Y126 (= Y112), K156 (= K140), Y161 (≠ R144), E190 (= E173), G193 (≠ M176), E194 (≠ T177), N195 (= N178), L214 (≠ V197), G216 (= G199), I217 (= I200), T218 (≠ I201), G253 (≠ N236), T254 (≠ S237), A255 (≠ I238)

1iydA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
29% identity, 95% coverage: 2:262/276 of query aligns to 5:282/304 of 1iydA

query
sites
1iydA

I

I

L

W

I

F

N

N

G

G

E

E

-

M

-

V

-

R

Y

W

R

E

D

D

-

A

H

K

I

V

E

H

I

V

S

M

D

S

R

H

G

A

F

L

Q

H

Y

Y

G

G

D

T

G

S

L

V

F
|
F

E

E

T
x
G

I

I

-

R

-

C

-

Y

E

D

V

S

H

H

N

K

G

G

R

P

P

V

V

V

F

F

L

R

N

H

R

R

-

E

H

H

L

M

H

Q

R
|
R

L

L

Q

H

T

D

G

S

C

A

A

K

R

I

L

Y

Q

R

I

F

P

P

C

V

P

S

D

Q

P

S

-

I

-

D

E

E

L

L

I

M

R

E

S

A

E

C

A

R

A

D

V

V

L

I

C

R

K

K

N

N

A

N

-

L

P

T

H

S

A

A

V
x
Y

L

I

K

R

L

P

I

L

I

I

T

F

R

V

G

G

S

D

G

V

G

G

R

M

G

G

Y

V

R

N

P

P

E

A

A

G

T

Y

R

S

P

T

T

D

R

V

V

I

L

I

S

A

L

A

H

F

P

P

C

W

P

G

D

A

Y
|
Y

P

L

G

G

-

A

R

E

Y

A

R

L

E

E

Q

Q

G

G

V

I

A

D

V

A

R

M

I

V

C

S

Q

S

-

W

T

N

R

R

L

A

G

A

L

P

N

N

P

T

A

I

L

P

A

T

G

A

I

A

K
|
K

H
x
A

L

G

-

G

N

N

R
x
Y

L

L

E

S

Q

S

V

L

L

L

A

V

R

G

A

S

E

E

W

A

D

R

P

H

E

G

I

Y

Q

Q

E

E

G

G

L

I

M

A

L

L

D

D

I

V

N

N

G

G

H

Y

V

I

I

S

E
|
E

G

G

T

A

M
x
G

T
x
E

N

N

L

L

F

F

Y

E

F

V

S

K

N

D

R

G

S

V

A

L

Y

F

T

T

P

P

A

P

L

F

T

T

Q

S

C

S

G

A

V
x
L

D

P

G
|
G

I
|
I

I
x
T

R

R

R

D

I

A

L

I

M

I

D

K

L

L

L

A

S

K

K

E

R

L

R

G

I

I

T

E

V

V

R

R

E

E

T

Q

A

V

P

L

T

S

V

R

D

E

E

S

L

L

S

Y

A

L

A

A

D

D

E

E

V

V

F

F

V

M

C

S

N

G

S
x
T

I
x
A

I

A

G

E

I

I

W

T

P

P

V

V

R

R

A

S

I

V

G

D

N

G

V

I

R

Q

Y

V

A

G

-

E

-

G

-

R

V

C

G

G

P

P

F

V

T

T

R

K

Q

R

C

I

Q

Q

active site: F33 (= F26), G35 (≠ T28), K156 (= K140), A157 (≠ H141), E190 (= E173), L214 (≠ V197)
binding glutaric acid: Y92 (≠ V78), Y126 (= Y112), A255 (≠ I238)
binding pyridoxal-5'-phosphate: R56 (= R45), K156 (= K140), Y161 (≠ R144), E190 (= E173), G193 (≠ M176), E194 (≠ T177), L214 (≠ V197), G216 (= G199), I217 (= I200), T218 (≠ I201), T254 (≠ S237)

1i1mA Crystal structure of escherichia coli branched-chain amino acid aminotransferase. (see paper)
29% identity, 95% coverage: 2:262/276 of query aligns to 5:282/304 of 1i1mA

query
sites
1i1mA

I

I

L

W

I

F

N

N

G

G

E

E

-

M

-

V

-

R

Y

W

R

E

D

D

-

A

H

K

I

V

E

H

I

V

S

M

D

S

R

H

G

A

F

L

Q

H

Y

Y

G

G

D

T

G

S

L

V

F

F

E

E

T

G

I

I

-

R

-

C

-

Y

E

D

V

S

H

H

N

K

G

G

R

P

P

V

V

V

F

F

L

R

N

H

R

R

-

E

H

H

L

M

H

Q

R
|
R

L

L

Q

H

T

D

G

S

C

A

A

K

R

I

L

Y

Q

R

I

F

P

P

C

V

P

S

D

Q

P

S

-

I

-

D

E

E

L

L

I

M

R

E

S

A

E

C

A

R

A

D

V

V

L

I

C

R

K

K

N

N

A

N

-

L

P

T

H

S

A

A

V
x
Y

L

I

K

R

L

P

I

L

I

I

T

F

R

V

G

G

S

D

G

V

G

G

R

M

G

G

Y

V

R

N

P

P

E

A

A

G

T

Y

R

S

P

T

T

D

R

V

V

I

L

I

S

A

L

A

H

F

P

P

C

W

P

G

D

A

Y

Y

P

L

G

G

-

A

R

E

Y

A

R

L

E

E

Q

Q

G

G

V

I

A

D

V

A

R

M

I

V

C

S

Q

S

-

W

T

N

R

R

L

A

G

A

L

P

N

N

P

T

A

I

L

P

A

T

G

A

I

A

K
|
K

H

A

L

G

-

G

N

N

R
x
Y

L

L

E

S

Q

S

V

L

L

L

A

V

R

G

A

S

E

E

W

A

D

R

P

H

E

G

I

Y

Q

Q

E

E

G

G

L

I

M

A

L

L

D

D

I

V

N

N

G

G

H

Y

V

I

I

S

E
|
E

G

G

T

A

M
x
G

T
x
E

N

N

L

L

F

F

Y

E

F

V

S

K

N

D

R

G

S

V

A

L

Y

F

T

T

P

P

A

P

L

F

T

T

Q

S

C

S

G

A

V
x
L

D

P

G
|
G

I
|
I

I
x
T

R

R

R

D

I

A

L

I

M

I

D

K

L

L

L

A

S

K

K

E

R

L

R

G

I

I

T

E

V

V

R

R

E

E

T

Q

A

V

P

L

T

S

V

R

D

E

E

S

L

L

S

Y

A

L

A

A

D

D

E

E

V

V

F

F

V

M

C

S

N
x
G

S
x
T

I
x
A

I

A

G

E

I

I

W

T

P

P

V

V

R

R

A

S

I

V

G

D

N

G

V

I

R

Q

Y

V

A

G

-

E

-

G

-

R

V

C

G

G

P

P

F

V

T

T

R

K

Q

R

C

I

Q

Q

active site: K156 (= K140)
binding 4-methyl valeric acid: Y92 (≠ V78), K156 (= K140), T254 (≠ S237), A255 (≠ I238)
binding pyridoxal-5'-phosphate: R56 (= R45), K156 (= K140), Y161 (≠ R144), E190 (= E173), G193 (≠ M176), E194 (≠ T177), L214 (≠ V197), G216 (= G199), I217 (= I200), T218 (≠ I201), G253 (≠ N236), T254 (≠ S237)

1i1lA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase. (see paper)
29% identity, 95% coverage: 2:262/276 of query aligns to 5:282/304 of 1i1lA

query
sites
1i1lA

I

I

L

W

I

F

N

N

G

G

E

E

-

M

-

V

-

R

Y

W

R

E

D

D

-

A

H

K

I

V

E

H

I

V

S

M

D

S

R

H

G

A

F

L

Q

H

Y

Y

G

G

D

T

G

S

L

V

F

F

E

E

T

G

I

I

-

R

-

C

-

Y

E

D

V

S

H

H

N

K

G

G

R

P

P

V

V

V

F

F

L

R

N

H

R

R

-

E

H

H

L

M

H

Q

R
|
R

L

L

Q

H

T

D

G

S

C

A

A

K

R

I

L

Y

Q

R

I

F

P

P

C

V

P

S

D

Q

P

S

-

I

-

D

E

E

L

L

I

M

R

E

S

A

E

C

A

R

A

D

V

V

L

I

C

R

K

K

N

N

A

N

-

L

P

T

H

S

A

A

V
x
Y

L

I

K

R

L

P

I

L

I

I

T

F

R

V

G

G

S

D

G

V

G

G

R

M

G

G

Y

V

R

N

P

P

E

A

A

G

T

Y

R

S

P

T

T

D

R

V

V

I

L

I

S

A

L

A

H

F

P

P

C

W

P

G

D

A

Y

Y

P

L

G

G

-

A

R

E

Y

A

R

L

E

E

Q

Q

G

G

V

I

A

D

V

A

R

M

I

V

C

S

Q

S

-

W

T

N

R

R

L

A

G

A

L

P

N

N

P

T

A

I

L

P

A

T

G

A

I

A

K
|
K

H

A

L

G

-

G

N

N

R
x
Y

L

L

E

S

Q

S

V

L

L

L

A

V

R

G

A

S

E

E

W

A

D

R

P

H

E

G

I

Y

Q

Q

E

E

G

G

L

I

M

A

L

L

D

D

I

V

N

N

G

G

H

Y

V

I

I

S

E
|
E

G

G

T

A

M
x
G

T

E

N

N

L

L

F

F

Y

E

F

V

S

K

N

D

R

G

S

V

A

L

Y

F

T

T

P

P

A

P

L

F

T

T

Q

S

C

S

G

A

V

L

D

P

G
|
G

I
|
I

I
x
T

R

R

R

D

I

A

L

I

M

I

D

K

L

L

L

A

S

K

K

E

R

L

R

G

I

I

T

E

V

V

R

R

E

E

T

Q

A

V

P

L

T

S

V

R

D

E

E

S

L

L

S

Y

A

L

A

A

D

D

E

E

V

V

F

F

V

M

C

S

N

G

S
x
T

I
x
A

I

A

G

E

I

I

W

T

P

P

V

V

R

R

A

S

I

V

G

D

N

G

V

I

R

Q

Y

V

A

G

-

E

-

G

-

R

V

C

G

G

P

P

F

V

T

T

R

K

Q

R

C

I

Q

Q

active site: K156 (= K140)
binding 2-methylleucine: Y92 (≠ V78), K156 (= K140), T254 (≠ S237), A255 (≠ I238)
binding pyridoxal-5'-phosphate: R56 (= R45), K156 (= K140), Y161 (≠ R144), E190 (= E173), G193 (≠ M176), G216 (= G199), I217 (= I200), T218 (≠ I201), T254 (≠ S237)

3lqsA Complex structure of d-amino acid aminotransferase and 4-amino-4,5- dihydro-thiophenecarboxylic acid (adta) (see paper)
28% identity, 91% coverage: 12:262/276 of query aligns to 17:269/280 of 3lqsA

query
sites
3lqsA

I

I

E

D

I

K

S

E

D

D

R

R

G

G

F

Y

Q

Q

Y

F

G

G

D

D

G

G

L

V

F
x
Y

E

E

T
x
V

I

V

E

K

V

V

H

Y

N

N

G

G

R

E

P

M

V

F

F

T

L

V

N

N

R

E

H

H

L

I

H

D

R
|
R

L

L

Q

Y

T

A

G

S

C

A

A

E

R

K

L

I

Q

R

I

I

P

T

C

I

P

P

D

Y

P

T

-

K

-

D

-

K

-

F

-

H

E

Q

L

L

I

L

R

H

S

E

E

L

A

V

A

E

V

K

L

N

C

E

K

L

N

N

A

T

P

G

H

H

A

I

V

Y

L

F

K

Q

L

-

I

-

I

V

T

T

R

R

G

G

S

T

G

S

G

P

R

R

G

A

Y

H

R

Q

P

F

P

P

E

E

A

N

T

T

R

V

P

K

T

P

R

V

V

I

L

I

S

G

L

Y

H

T

P

K

C

E

P

N

D

P

Y

R

P

P

G

L

R

E

Y

N

R

L

E

E

Q

K

G

G

V

V

A

K

V

A

R

T

I

F

C

V

Q

E

T

D

R

I

L

R

G

W

L

L

N

R

P

-

A

-

L

-

A

C

G

D

I

I

K
|
K

H

S

L

L

N

N

R

L

L

L

E

G

Q

A

V

V

L

L

A

A

R

K

A

Q

E

E

W

A

D

H

D

E

P

K

E

G

I

C

Q

Y

E

E

G

A

L

I

M

-

L

L

D

H

I

R

N

N

G

N

H

T

V

V

I

T

E
|
E

G

G

T

S

M
x
S

T
x
S

N
|
N

L

V

F

F

Y

G

F

I

S

K

N

D

R

G

S

I

A

L

Y

Y

T

T

P

H

A

P

L

A

T

N

Q

N

C

M

G

I

V
x
L

D

K

G
|
G

I
|
I

I
x
T

R

R

R

D

I

V

L

V

M

I

D

A

L

C

L

A

S

N

K

E

R

I

R

N

I

M

T

P

V

V

R

K

E

E

T

I

A

P

P

F

T

T

V

T

D

H

E

E

L

A

S

L

A

K

A

M

D

D

E

E

V

L

F

F

V

V

C

T

N
x
S

S
x
T

I
x
T

I

S

G

E

I

I

W

T

P

P

V

V

R

I

A

E

I

I

G

D

N

G

-

K

-

L

V

I

R

R

Y

D

A

G

-

K

V

V

G

G

P

E

F

W

T

T

R

R

Q

K

C

L

Q

Q

active site: Y31 (≠ F26), V33 (≠ T28), K145 (= K140), E177 (= E173), L201 (≠ V197)
binding 4-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]thiophene-2-carboxylic acid: V33 (≠ T28), R50 (= R45), E177 (= E173), S180 (≠ M176), S181 (≠ T177), N182 (= N178), L201 (≠ V197), G203 (= G199), I204 (= I200), T205 (≠ I201), S240 (≠ N236), T241 (≠ S237), T242 (≠ I238)

P19938 D-alanine aminotransferase; D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase; EC 2.6.1.21 from Bacillus sp. (strain YM-1) (see 5 papers)
28% identity, 91% coverage: 12:262/276 of query aligns to 18:270/283 of P19938

query
sites
P19938

I

I

E

D

I

K

S

E

D

D

R

R

G

G

F

Y

Q

Q

Y

F

G

G

D

D

G

G

L

V

F
x
Y

E

E

T

V

I

V

E

K

V

V

H

Y

N

N

G

G

R

E

P

M

V

F

F

T

L

V

N

N

R

E

H

H

L

I

H

D

R
|
R

L

L

Q

Y

T

A

G

S

C

A

A

E

R

K

L

I

Q

R

I

I

P

T

C

I

P

P

D

Y

P

T

-

K

-

D

-

K

-

F

-

H

E

Q

L

L

I

L

R

H

S

E

E

L

A

V

A

E

V

K

L

N

C

E

K

L

N

N

A

T

P

G

H

H

A

I

V

Y

L

F

K

Q

L

-

I

-

I

V

T

T

R

R

G

G

S

T

G

S

G

P

R
|
R

G

A

Y
x
H

R

Q

P

F

P

P

E

E

A

N

T

T

R

V

P

K

T

P

R

V

V

I

L

I

S

G

L

Y

H

T

P

K

C

E

P

N

D

P

Y

R

P

P

G

L

R

E

Y

N

R

L

E

E

Q

K

G

G

V

V

A

K

V

A

R

T

I

F

C

V

Q

E

T

D

R

I

L

R

G

W

L

L

N

R

P

-

A

-

L

-

A

C

G

D

I

I

K
|
K

H

S

L

L

N

N

R

L

L

L

E

G

Q

A

V

V

L

L

A

A

R

K

A

Q

E

E

W

A

D

H

D

E

P

K

E

G

I

C

Q

Y

E

E

G

A

L

I

M

-

L

L

D

H

I

R

N

N

G

N

H

T

V

V

I

T

E
|
E

G

G

T

S

M

S

T

S

N

N

L

V

F

F

Y

G

F

I

S

K

N

D

R

G

S

I

A

L

Y

Y

T

T

P

H

A

P

L

A

T

N

Q

N

C

M

G

I

V
x
L

D

K

G

G

I

I

I

T

R

R

R

D

I

V

L

V

M

I

D

A

L

C

L

A

S

N

K

E

R

I

R

N

I

M

T

P

V

V

R

K

E

E

T

I

A

P

P

F

T

T

V

T

D

H

E

E

L

A

S

L

A

K

A

M

D

D

E

E

V

L

F

F

V

V

C

T

N

S

S

T

I

T

I

S

G

E

I

I

W

T

P

P

V

V

R

I

A

E

I

I

G

D

N

G

-

K

-

L

V

I

R

R

Y

D

A

G

-

K

V

V

G

G

P

E

F

W

T

T

R

R

Q

K

C

L

Q

Q

Y32 (≠ F26) binding
R51 (= R45) binding
R99 (= R90) binding
H101 (≠ Y92) binding
K146 (= K140) active site, Proton acceptor; modified: N6-(pyridoxal phosphate)lysine
E178 (= E173) binding ; mutation to K: Loss of transaminase activity and small gain in racemase activity.
L202 (≠ V197) mutation to A: Inactivates enzyme.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

3daaA Crystallographic structure of d-amino acid aminotransferase inactivated by pyridoxyl-d-alanine (see paper)
28% identity, 91% coverage: 12:262/276 of query aligns to 17:269/277 of 3daaA

query
sites
3daaA

I

I

E

D

I

K

S

E

D

D

R

R

G

G

F

Y

Q

Q

Y

F

G

G

D

D

G

G

L

V

F
x
Y

E

E

T
x
V

I

V

E

K

V

V

H

Y

N

N

G

G

R

E

P

M

V

F

F

T

L

V

N

N

R

E

H

H

L

I

H

D

R
|
R

L

L

Q

Y

T

A

G

S

C

A

A

E

R

K

L

I

Q

R

I

I

P

T

C

I

P

P

D

Y

P

T

-

K

-

D

-

K

-

F

-

H

E

Q

L

L

I

L

R

H

S

E

E

L

A

V

A

E

V

K

L

N

C

E

K

L

N

N

A

T

P

G

H

H

A

I

V

Y

L

F

K

Q

L

-

I

-

I

V

T

T

R

R

G

G

S

T

G

S

G

P

R

R

G

A

Y

H

R

Q

P

F

P

P

E

E

A

N

T

T

R

V

P

K

T

P

R

V

V

I

L

I

S

G

L

Y

H

T

P

K

C

E

P

N

D

P

Y

R

P

P

G

L

R

E

Y

N

R

L

E

E

Q

K

G

G

V

V

A

K

V

A

R

T

I

F

C

V

Q

E

T

D

R

I

L

R

G

W

L

L

N

R

P

-

A

-

L

-

A

C

G

D

I

I

K
|
K

H

S

L

L

N

N

R

L

L

L

E

G

Q

A

V

V

L

L

A

A

R

K

A

Q

E

E

W

A

D

H

D

E

P

K

E

G

I

C

Q

Y

E

E

G

A

L

I

M

-

L

L

D

H

I

R

N

N

G

N

H

T

V

V

I

T

E
|
E

G

G

T

S

M
x
S

T
x
S

N

N

L

V

F

F

Y

G

F

I

S

K

N

D

R

G

S

I

A

L

Y

Y

T

T

P

H

A

P

L

A

T

N

Q

N

C

M

G

I

V
x
L

D

K

G
|
G

I
|
I

I
x
T

R

R

R

D

I

V

L

V

M

I

D

A

L

C

L

A

S

N

K

E

R

I

R

N

I

M

T

P

V

V

R

K

E

E

T

I

A

P

P

F

T

T

V

T

D

H

E

E

L

A

S

L

A

K

A

M

D

D

E

E

V

L

F

F

V

V

C

T

N
x
S

S
x
T

I

T

I

S

G

E

I

I

W

T

P

P

V

V

R

I

A

E

I

I

G

D

N

G

-

K

-

L

V

I

R

R

Y

D

A

G

-

K

V

V

G

G

P

E

F

W

T

T

R

R

Q

K

C

L

Q

Q

active site: Y31 (≠ F26), V33 (≠ T28), K145 (= K140), E177 (= E173), L201 (≠ V197)
binding n-(5'-phosphopyridoxyl)-d-alanine: Y31 (≠ F26), R50 (= R45), K145 (= K140), E177 (= E173), S180 (≠ M176), S181 (≠ T177), L201 (≠ V197), G203 (= G199), I204 (= I200), T205 (≠ I201), S240 (≠ N236), T241 (≠ S237)

2daaA Crystallographic structure of d-amino acid aminotransferase inactivated by d-cycloserine
28% identity, 91% coverage: 12:262/276 of query aligns to 17:269/277 of 2daaA

query
sites
2daaA

I

I

E

D

I

K

S

E

D

D

R

R

G

G

F

Y

Q

Q

Y

F

G

G

D

D

G

G

L

V

F
x
Y

E

E

T
x
V

I

V

E

K

V

V

H

Y

N

N

G

G

R

E

P

M

V

F

F

T

L

V

N

N

R

E

H

H

L

I

H

D

R
|
R

L

L

Q

Y

T

A

G

S

C

A

A

E

R

K

L

I

Q

R

I

I

P

T

C

I

P

P

D

Y

P

T

-

K

-

D

-

K

-

F

-

H

E

Q

L

L

I

L

R

H

S

E

E

L

A

V

A

E

V

K

L

N

C

E

K

L

N

N

A

T

P

G

H

H

A

I

V

Y

L

F

K

Q

L

-

I

-

I

V

T

T

R

R

G

G

S

T

G

S

G

P

R
|
R

G

A

Y
x
H

R

Q

P

F

P

P

E

E

A

N

T

T

R

V

P

K

T

P

R

V

V

I

L

I

S

G

L

Y

H

T

P

K

C

E

P

N

D

P

Y

R

P

P

G

L

R

E

Y

N

R

L

E

E

Q

K

G

G

V

V

A

K

V

A

R

T

I

F

C

V

Q

E

T

D

R

I

L

R

G

W

L

L

N

R

P

-

A

-

L

-

A

C

G

D

I

I

K
|
K

H

S

L

L

N

N

R

L

L

L

E

G

Q

A

V

V

L

L

A

A

R

K

A

Q

E

E

W

A

D

H

D

E

P

K

E

G

I

C

Q

Y

E

E

G

A

L

I

M

-

L

L

D

H

I

R

N

N

G

N

H

T

V

V

I

T

E
|
E

G

G

T

S

M
x
S

T
x
S

N
|
N

L

V

F

F

Y

G

F

I

S

K

N

D

R

G

S

I

A

L

Y

Y

T

T

P

H

A

P

L

A

T

N

Q

N

C

M

G

I

V
x
L

D

K

G
|
G

I
|
I

I
x
T

R

R

R

D

I

V

L

V

M

I

D

A

L

C

L

A

S

N

K

E

R

I

R

N

I

M

T

P

V

V

R

K

E

E

T

I

A

P

P

F

T

T

V

T

D

H

E

E

L

A

S

L

A

K

A

M

D

D

E

E

V

L

F

F

V

V

C

T

N

S

S
x
T

I

T

I

S

G

E

I

I

W

T

P

P

V

V

R

I

A

E

I

I

G

D

N

G

-

K

-

L

V

I

R

R

Y

D

A

G

-

K

V

V

G

G

P

E

F

W

T

T

R

R

Q

K

C

L

Q

Q

active site: Y31 (≠ F26), V33 (≠ T28), K145 (= K140), E177 (= E173), L201 (≠ V197)
binding d-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-n,o-cycloserylamide: Y31 (≠ F26), V33 (≠ T28), R50 (= R45), R98 (= R90), H100 (≠ Y92), K145 (= K140), E177 (= E173), S180 (≠ M176), S181 (≠ T177), N182 (= N178), L201 (≠ V197), G203 (= G199), I204 (= I200), T205 (≠ I201), T241 (≠ S237)

1daaA Crystallographic structure of d-amino acid aminotransferase complexed with pyridoxal-5'-phosphate (see paper)
28% identity, 91% coverage: 12:262/276 of query aligns to 17:269/277 of 1daaA

query
sites
1daaA

I

I

E

D

I

K

S

E

D

D

R

R

G

G

F

Y

Q

Q

Y

F

G

G

D

D

G

G

L

V

F
x
Y

E

E

T
x
V

I

V

E

K

V

V

H

Y

N

N

G

G

R

E

P

M

V

F

F

T

L

V

N

N

R

E

H

H

L

I

H

D

R
|
R

L

L

Q

Y

T

A

G

S

C

A

A

E

R

K

L

I

Q

R

I

I

P

T

C

I

P

P

D

Y

P

T

-

K

-

D

-

K

-

F

-

H

E

Q

L

L

I

L

R

H

S

E

E

L

A

V

A

E

V

K

L

N

C

E

K

L

N

N

A

T

P

G

H

H

A

I

V

Y

L

F

K

Q

L

-

I

-

I

V

T

T

R

R

G

G

S

T

G

S

G

P

R

R

G

A

Y

H

R

Q

P

F

P

P

E

E

A

N

T

T

R

V

P

K

T

P

R

V

V

I

L

I

S

G

L

Y

H

T

P

K

C

E

P

N

D

P

Y

R

P

P

G

L

R

E

Y

N

R

L

E

E

Q

K

G

G

V

V

A

K

V

A

R

T

I

F

C

V

Q

E

T

D

R

I

L

R

G

W

L

L

N

R

P

-

A

-

L

-

A

C

G

D

I

I

K
|
K

H

S

L

L

N

N

R

L

L

L

E

G

Q

A

V

V

L

L

A

A

R

K

A

Q

E

E

W

A

D

H

D

E

P

K

E

G

I

C

Q

Y

E

E

G

A

L

I

M

-

L

L

D

H

I

R

N

N

G

N

H

T

V

V

I

T

E
|
E

G

G

T

S

M
x
S

T
x
S

N

N

L

V

F

F

Y

G

F

I

S

K

N

D

R

G

S

I

A

L

Y

Y

T

T

P

H

A

P

L

A

T

N

Q

N

C

M

G

I

V
x
L

D

K

G
|
G

I
|
I

I
x
T

R

R

R

D

I

V

L

V

M

I

D

A

L

C

L

A

S

N

K

E

R

I

R

N

I

M

T

P

V

V

R

K

E

E

T

I

A

P

P

F

T

T

V

T

D

H

E

E

L

A

S

L

A

K

A

M

D

D

E

E

V

L

F

F

V

V

C

T

N
x
S

S
x
T

I

T

I

S

G

E

I

I

W

T

P

P

V

V

R

I

A

E

I

I

G

D

N

G

-

K

-

L

V

I

R

R

Y

D

A

G

-

K

V

V

G

G

P

E

F

W

T

T

R

R

Q

K

C

L

Q

Q

active site: Y31 (≠ F26), V33 (≠ T28), K145 (= K140), E177 (= E173), L201 (≠ V197)
binding pyridoxal-5'-phosphate: R50 (= R45), K145 (= K140), E177 (= E173), S180 (≠ M176), S181 (≠ T177), L201 (≠ V197), G203 (= G199), I204 (= I200), T205 (≠ I201), S240 (≠ N236), T241 (≠ S237)

1a0gB L201a mutant of d-amino acid aminotransferase complexed with pyridoxamine-5'-phosphate (see paper)
28% identity, 91% coverage: 12:262/276 of query aligns to 17:269/282 of 1a0gB

query
sites
1a0gB

I

I

E

D

I

K

S

E

D

D

R

R

G

G

F

Y

Q

Q

Y

F

G

G

D

D

G

G

L

V

F
x
Y

E

E

T
x
V

I

V

E

K

V

V

H

Y

N

N

G

G

R

E

P

M

V

F

F

T

L

V

N

N

R

E

H

H

L

I

H

D

R
|
R

L

L

Q

Y

T

A

G

S

C

A

A

E

R

K

L

I

Q

R

I

I

P

T

C

I

P

P

D

Y

P

T

-

K

-

D

-

K

-

F

-

H

E

Q

L

L

I

L

R

H

S

E

E

L

A

V

A

E

V

K

L

N

C

E

K

L

N

N

A

T

P

G

H

H

A

I

V

Y

L

F

K

Q

L

-

I

-

I

V

T

T

R

R

G

G

S

T

G

S

G

P

R

R

G

A

Y

H

R

Q

P

F

P

P

E

E

A

N

T

T

R

V

P

K

T

P

R

V

V

I

L

I

S

G

L

Y

H

T

P

K

C

E

P

N

D

P

Y

R

P

P

G

L

R

E

Y

N

R

L

E

E

Q

K

G

G

V

V

A

K

V

A

R

T

I

F

C

V

Q

E

T

D

R

I

L

R

G

W

L

L

N

R

P

-

A

-

L

-

A

C

G

D

I

I

K
|
K

H

S

L

L

N

N

R

L

L

L

E

G

Q

A

V

V

L

L

A

A

R

K

A

Q

E

E

W

A

D

H

D

E

P

K

E

G

I

C

Q

Y

E

E

G

A

L

I

M

-

L

L

D

H

I

R

N

N

G

N

H

T

V

V

I

T

E
|
E

G

G

T

S

M
x
S

T
x
S

N

N

L

V

F

F

Y

G

F

I

S

K

N

D

R

G

S

I

A

L

Y

Y

T

T

P

H

A

P

L

A

T

N

Q

N

C

M

G

I

V
x
A

D

K

G
|
G

I
|
I

I
x
T

R

R

R

D

I

V

L

V

M

I

D

A

L

C

L

A

S

N

K

E

R

I

R

N

I

M

T

P

V

V

R

K

E

E

T

I

A

P

P

F

T

T

V

T

D

H

E

E

L

A

S

L

A

K

A

M

D

D

E

E

V

L

F

F

V

V

C

T

N
x
S

S
x
T

I

T

I

S

G

E

I

I

W

T

P

P

V

V

R

I

A

E

I

I

G

D

N

G

-

K

-

L

V

I

R

R

Y

D

A

G

-

K

V

V

G

G

P

E

F

W

T

T

R

R

Q

K

C

L

Q

Q

active site: Y31 (≠ F26), V33 (≠ T28), K145 (= K140), E177 (= E173), A201 (≠ V197)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R50 (= R45), K145 (= K140), E177 (= E173), S180 (≠ M176), S181 (≠ T177), G203 (= G199), I204 (= I200), T205 (≠ I201), S240 (≠ N236), T241 (≠ S237)

8pnyA Crystal structure of d-amino acid aminotransferase from blastococcus saxobsidens complexed with phenylhydrazine and in its apo form (see paper)
27% identity, 89% coverage: 16:262/276 of query aligns to 26:274/278 of 8pnyA

query
sites
8pnyA

D

D

R

L

G

G

F

L

Q

G

Y

R

G

G

D

D

G

G

L

I

F
|
F

E

E

T
x
S

I

V

E

A

V

V

H

V

N

A

G

G

R

R

P

T

V

P

F

H

L

L

N

A

R

A

H

H

L

L

H

T

R
|
R

L

L

Q

T

T

R

G

S

C

A

A

A

R

L

L

L

Q

G

I

L

P

P

C

A

P

P

D

G

P

D

E

Q

L

A

I

W

R

M

S

E

E

M

A

V

A

A

V

A

L

V

C

L

K

A

N

D

A

W

P

P

H

A

A

A

-

L

-

E

-

G

V

V

L

C

K

R

L

L

I

F

I

L

T

T

R

R

G

G

S

L

G

G

G

-

R

-

G

-

Y

-

R

-

P

-

E

D

A

G

T

T

R

P

P

P

T

T

R

A

V

L

L

A

S

L

L

L

H

A

P

P

C

V

P

P

D

A

Y

D

P

T

G

L

R

R

Y

Q

R

R

E

A

Q

E

G

G

V

I

A

S

V

V

R

A

I

T

-

L

-

G

-

L

-

G

-

V

-

P

C

A

Q

D

T

F

R

R

L

A

G

G

L

A

N

P

P

W

A

L

L

L

A

G

G

G

I

A

K
|
K

H

T

L

L

N

S

R
x
Y

L

A

E

V

Q

N

V

M

L

A

A

A

R

Q

A

R

E

H

W

A

D

H

D

D

P

L

E

G

I

A

Q

D

E

D

G

V

L

V

M

F

L

T

D

S

I

L

N

E

G

G

H

R

V

L

I

L

E
|
E

G
|
G

T
x
P

M
x
T

T
x
S

N
x
T

L

V

F

V

Y

W

F

A

S

A

N

G

R

G

S

T

A

L

Y

H

T

T

P

P

A

P

L

V

T

E

Q

T

C

G

G

I

V
x
L

D

P

G

G

I
x
T

R

Q

R

A

I

R

L

L

M

F

D

T

L

A

S

A

K

A

R

D

R

G

I

W

T

P

V

T

R

S

E

V

T

T

A

P

P

G

T

T

V

V

D

D

E

D

L

L

S

H

A

A

D

D

E

A

V

V

F

W

V

L

C

L

N
x
S

S
x
G

I

V

I

R

G

G

I

A

W

A

P

V

V

V

R

H

A

T

I

V

G

D

N

G

V

V

R

R

Y

R

A

G

V

D

G

G

P

D

F

L

T

S

R

R

Q

R

C

V

Q

R

binding [6-methyl-5-oxidanyl-4-[(2-phenylhydrazinyl)methyl]pyridin-3-yl]methyl dihydrogen phosphate: F36 (= F26), S38 (≠ T28), R55 (= R45), K152 (= K140), Y156 (≠ R144), E185 (= E173), G186 (= G174), P187 (≠ T175), T188 (≠ M176), S189 (≠ T177), T190 (≠ N178), L209 (≠ V197), T212 (≠ I200), T213 (≠ I201), S248 (≠ N236), G249 (≠ S237)

8pnwA Crystal structure of d-amino acid aminotransferase from blastococcus saxobsidens in holo form with plp (see paper)
27% identity, 89% coverage: 16:262/276 of query aligns to 26:274/278 of 8pnwA

query
sites
8pnwA

D

D

R

L

G

G

F

L

Q

G

Y

R

G

G

D

D

G

G

L

I

F

F

E

E

T

S

I

V

E

A

V

V

H

V

N

A

G

G

R

R

P

T

V

P

F

H

L

L

N

A

R

A

H

H

L

L

H

T

R
|
R

L

L

Q

T

T

R

G

S

C

A

A

A

R

L

L

L

Q

G

I

L

P

P

C

A

P

P

D

G

P

D

E

Q

L

A

I

W

R

M

S

E

E

M

A

V

A

A

V

A

L

V

C

L

K

A

N

D

A

W

P

P

H

A

A

A

-

L

-

E

-

G

V

V

L

C

K

R

L

L

I

F

I

L

T

T

R

R

G

G

S

L

G

G

G

-

R

-

G

-

Y

-

R

-

P

-

E

D

A

G

T

T

R

P

P

P

T

T

R

A

V

L

L

A

S

L

L

L

H

A

P

P

C

V

P

P

D

A

Y

D

P

T

G

L

R

R

Y

Q

R

R

E

A

Q

E

G

G

V

I

A

S

V

V

R

A

I

T

-

L

-

G

-

L

-

G

-

V

-

P

C

A

Q

D

T

F

R

R

L

A

G

G

L

A

N

P

P

W

A

L

L

L

A

G

G

G

I

A

K
|
K

H

T

L

L

N

S

R
x
Y

L

A

E

V

Q

N

V

M

L

A

A

A

R

Q

A

R

E

H

W

A

D

H

D

D

P

L

E

G

I

A

Q

D

E

D

G

V

L

V

M

F

L

T

D

S

I

L

N

E

G

G

H

R

V

L

I

L

E
|
E

G

G

T
x
P

M
x
T

T
x
S

N

T

L

V

F

V

Y

W

F

A

S

A

N

G

R

G

S

T

A

L

Y

H

T

T

P

P

A

P

L

V

T

E

Q

T

C

G

G

I

V
x
L

D

P

G

G

I
x
T

R

Q

R

A

I

R

L

L

M

F

D

T

L

A

S

A

K

A

R

D

R

G

I

W

T

P

V

T

R

S

E

V

T

T

A

P

P

G

T

T

V

V

D

D

E

D

L

L

S

H

A

A

D

D

E

A

V

V

F

W

V

L

C

L

N

S

S
x
G

I

V

I

R

G

G

I

A

W

A

P

V

V

V

R

H

A

T

I

V

G

D

N

G

V

V

R

R

Y

R

A

G

V

D

G

G

P

D

F

L

T

S

R

R

Q

R

C

V

Q

R

binding pyridoxal-5'-phosphate: R55 (= R45), K152 (= K140), Y156 (≠ R144), E185 (= E173), P187 (≠ T175), T188 (≠ M176), S189 (≠ T177), L209 (≠ V197), T212 (≠ I200), T213 (≠ I201), G249 (≠ S237)

7p3tB Transaminase of gamma-proteobacterium (see paper)
29% identity, 90% coverage: 1:248/276 of query aligns to 5:259/299 of 7p3tB

query
sites
7p3tB

M

I

I

I

L

Y

I

I

N

N

G

G

E

D

Y

Y

-

L

-

P

-

L

-

S

R

Q

D

A

H

R

I

V

E

S

I

P

S

V

D

D

R

Q

G

G

F

F

Q

L

Y

L

G

G

D

D

G

G

L

V

F

F

E

D

T

V

I

V

E

S

V

A

H

W

N

K

G

G

R

N

P

I

V

F

F

K

L

L

N

D

R

A

H

H

L

L

H

D

R
|
R

L

F

-

F

-

D

Q

S

T

I

G

Q

C

A

A

A

R

R

L

L

Q

N

I

H

P

D

C

M

P

S

D

R

P

D

E

A

L

W

I

K

R

E

S

A

E

I

A

I

A

E

V

T

L

T

C

R

K

R

N

N

A

G

-

L

P

D

H

D

A

A

V

S

L

I

K

R

L

F

I

I

I

V

T

T

R

R

G

G

S

E

G

P

G

K

R

G

G

V

Y

V

R

A

P

D

P

P

E

R

A

D

T

F

R

K

P

P

T

T

R

C

V

I

L

V

S

W

L

V

H

A

P

P

C

Y

P

I

D

F

Y

L

P

A

G

D

R

E

-

E

Y

K

R

R

E

R

Q

N

G

G

V

I

A

R

V

L

R

M

I

I

C

S

Q

A

T

T

R

R

-

G

-

F

-

P

-

A

L

D

G

T

L

L

N

D

P

P

A

R

L

Y

A

-

G

-

I

-

K
|
K

H

C

L

L

N

D

R
|
R

L

L

E

H

Q

S

V

Q

L

L

A

I

R

R

A

L

E

E

W

A

D

L

D

E

P

A

E

G

I

Y

Q

D

E

D

G

A

L

L

M

W

L

L

D

D

I

H

N

S

G

G

H

H

V

V

I

S

E
|
E

G
x
S

T
x
A

M
x
A

T
x
S

N

N

L

L

F

F

Y

I

F

V

S

K

N

N

R

G

S

V

A

L

Y

Y

T

T

P

P

A

S

L

-

T

-

Q

-

C

A

G

G

V

I

-

L

D

R

G
|
G

I
|
I

I
x
T

R

R

R

D

I

T

L

I

M

L

D

E

L

L

L

A

S

T

K

E

R

L

R

D

I

I

T

P

V

W

R

K

E

E

T

R

A

Q

P

L

T

S

V

A

D

F

E

D

L

V

S

Y

A

I

A

A

D

D

E

E

V

V

F

F

V

T

C

C

N

S

S
x
T

I

A

I

G

G

G

I

A

W

L

P

P

V

V

R

R

A

E

I

V

G

A

binding pyridoxal-5'-phosphate: R53 (= R45), K153 (= K140), R157 (= R144), E186 (= E173), S187 (≠ G174), A188 (≠ T175), A189 (≠ M176), S190 (≠ T177), G210 (= G199), I211 (= I200), T212 (≠ I201), T248 (≠ S237)

4whxA X-ray crystal structure of an amino acid aminotransferase from burkholderia pseudomallei bound to the co-factor pyridoxal phosphate
29% identity, 96% coverage: 7:270/276 of query aligns to 17:296/306 of 4whxA

query
sites
4whxA

E

E

Y

W

R

R

D

D

-

A

H

K

I

I

E

H

I

V

S

L

D

T

R

H

G

T

F

L

Q

H

Y

Y

G

G

D

M

G

G

L

V

F
|
F

E

E

T

G

I

V

E

R

V

A

H

Y

N

K

-

T

-

A

-

D

-

G

G

G

R

T

P

A

V

I

F

F

-

R

L

L

N

K

R

E

H

H

L

T

H

K

R

R

L

L

Q

L

T

N

G

S

C

A

A

K

R

I

L

F

Q

Q

I

M

P

D

C

V

P

P

-

F

D

D

P

Q

E

E

L

T

I

L

R

E

S

A

-

A

E

Q

A

R

A

D

V

V

L

V

C

R

K

E

N

N

A

K

P

L

H

E

A

S

-

C

V
x
Y

L

L

K

R

L

P

I

I

T

W

R

I

G

G

S

S

G

E

G

K

R

L

G

G

Y

V

R

S

P

A

P

K

E

G

A

N

T

T

R

I

P

H

T

V

R

A

V

I

L

A

S

A

L

W

H

-

P

P

C

W

P

G

D

A

Y

Y

P

L

G

G

R

-

Y

-

R

-

E

E

Q

E

G

G

V

L

A

A

-

K

-

G

V

I

R

R

I

V

C

K

Q

T

-

S

-

F

T

T

R

R

L

H

G

H

L

V

N

N

P

V

A

S

L

M

A

V

G

R

I

A

K
|
K

H

A

L

S

N

G

R

W

-

Y

L

V

E

N

Q

S

V

I

L

L

A

A

R

N

A

Q

E

E

W

A

D

T

D

A

P

D

E

G

I

Y

Q

D

E

E

G

A

L

L

M

L

L

L

D

D

I

V

N

D

G

G

H

Y

V

V

I

S

E
|
E

G

G

T

S

M

G

T

E

N

N

L

F

F

F

Y

L

F

V

S

N

N

R

R

G

S

K

A

L

Y

Y

T

T

P

P

A

D

L

L

T

A

Q

S

C

C

G

-

V
x
L

D

D

G

G

I

I

I

T

R

R

R

D

I

T

L

V

M

I

D

T

L

L

L

A

S

K

K

E

R

A

R

G

I

I

T

E

V

V

R

I

E

E

T

K

A

R

P

I

T

T

V

R

D

D

E

E

L

V

S

Y

A

T

A

A

D

D

E

E

V

A

F

F

V

F

C

T

N

G

S

T

I
x
A

I

A

G

E

I

V

W

T

P

P

V

I

R

R

A

E

I

L

G

D

N

N

V

R

R

T

Y

I

-

G

A

A

V

R

G

G

P

P

F

I

T

T

R

E

Q

K

C

L

Q

Q

-

S

-

A

-

F

F

F

D

D

L

V

T

V

E

N

F

G

K

K

N

S

active site: F37 (= F26), K160 (= K140), E194 (= E173), L217 (≠ V197)
binding alanine: Y97 (≠ V78), K160 (= K140), A258 (≠ I238)

5k3wA Structural characterisation of fold iv-transaminase, cputa1, from curtobacterium pusillum (see paper)
26% identity, 88% coverage: 11:254/276 of query aligns to 31:278/298 of 5k3wA

query
sites
5k3wA

H

Q

I

V

E

R

I

I

S

T

D

D

R

L

G

G

F

I

Q

T

Y

R

G

G

D

D

G

G

L

V

F

F

E

E

T

T

I

I

E

A

V

V

H

I

N

D

G

G

R

H

P

P

V

Q

F

A

L

L

N

E

R

L

H

H

L

L

H

G

R
|
R

L

L

Q

A

T

H

G

S

C

A

A

A

R

L

L

L

Q

D

I

L

P

P

C

E

P

P

D

D

P

A

E

A

L

V

I

W

R

R

S

E

E

-

A

-

A

A

V

V

L

L

C

A

K

G

N

V

A

A

P

D

H

Y

-

R

-

S

-

R

-

N

-

G

-

D

-

G

-

E

A

L

V

F

L

A

K

K

L

L

I

I

I

L

T

T

R

R

G

G

S

I

G

E

G

G

R

E

G

G

Y

-

R

-

P

-

E

-

A

-

T

-

R

R

P

P

T

S

R

G

V

W

L

V

S

F

L

V

H

D

P

E

C

G

P

E

D

D

Y

F

P

S

G

-

R

-

Y

-

R

-

E

Q

Q

Q

G

R

V

L

A

G

V

I

R

R

I

V

C

V

Q

T

T

L

R

D

L

R

G

G

L

Y

-

R

-

H

-

D

-

V

-

A

-

E

-

T

N

S

P

P

-

W

A

L

L

L

A

A

G

G

I

A

K
|
K

H

S

L

L

N

S

R
x
Y

L

A

E

T

Q

N

V

R

L

A

A

A

R

G

A

R

E

E

W

A

D

A

D

R

P

R

E

G

I

A

Q

D

E

D

G

V

L

I

M

F

L

V

D

S

I

S

N

D

G

G

H

Y

V

A

I

L

E
|
E

G

G

T
x
P

M
x
T

T
x
S

N
|
N

L

V

F

I

Y

V

F

L

S

A

N

D

R

G

S

V

A

V

Y

R

T

T

P

P

A

Q

L

T

T

D

Q

Q

C

G

G

I

V
x
L

D

A

G

G

I
x
T

R

Q

R

A

I

A

L

V

M

F

D

D

L

F

S

E

K

E

R

R

R

G

I

Y

T

P

V

T

R

E

E

Y

T

R

A

R

P

I

T

S

V

A

D

D

E

E

L

L

S

R

A

D

A

A

D

E

E

A

V

L

F

W

V

L

C

V

N

S

S
|
S

I

V

I

R

G

Q

I

A

W

A

P

P

V

I

R

T

A

A

I

L

G

D

N

D

V

R

R

E

Y

Y

A

P

V

V

binding pyridoxal-5'-phosphate: R65 (= R45), K164 (= K140), Y168 (≠ R144), E197 (= E173), P199 (≠ T175), T200 (≠ M176), S201 (≠ T177), N202 (= N178), L221 (≠ V197), T224 (≠ I200), T225 (≠ I201), S261 (= S237)

Query Sequence

>WP_020563279.1 NCBI__GCF_000372865.1:WP_020563279.1
MILINGEYRDHIEISDRGFQYGDGLFETIEVHNGRPVFLNRHLHRLQTGCARLQIPCPDP
ELIRSEAAVLCKNAPHAVLKLIITRGSGGRGYRPPEATRPTRVLSLHPCPDYPGRYREQG
VAVRICQTRLGLNPALAGIKHLNRLEQVLARAEWDDPEIQEGLMLDINGHVIEGTMTNLF
YFSNRSAYTPALTQCGVDGIIRRILMDLLSKRRITVRETAPTVDELSAADEVFVCNSIIG
IWPVRAIGNVRYAVGPFTRQCQFDLTEFKNEAVHDE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory