SitesBLAST
Comparing WP_020563736.1 NCBI__GCF_000372865.1:WP_020563736.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
7djsD Crystal structure of isopiperitenol dehydrogenase from pseudomonas aeruginosa complexed with NAD
48% identity, 98% coverage: 5:252/254 of query aligns to 3:249/251 of 7djsD
- binding nicotinamide-adenine-dinucleotide: G12 (= G14), G16 (= G18), I17 (= I19), D36 (= D38), L37 (≠ I39), C61 (= C63), D62 (= D64), V63 (= V65), N89 (= N91), A90 (= A92), T140 (= T143), S142 (= S145), Y155 (= Y158), K159 (= K162), A186 (≠ G189), V187 (≠ Y190)
8y83A Crystal structure of a ketoreductase from sphingobacterium siyangense sy1 with co-enzyme (see paper)
44% identity, 97% coverage: 7:252/254 of query aligns to 6:247/249 of 8y83A
- binding nicotinamide-adenine-dinucleotide: G13 (= G14), S16 (≠ N17), G17 (= G18), I18 (= I19), D37 (= D38), I38 (= I39), A62 (≠ C63), D63 (= D64), S64 (≠ V65), N90 (= N91), M141 (≠ T143), Y156 (= Y158), K160 (= K162), P186 (= P188), G187 (= G189), Y188 (= Y190), I189 (= I191), L193 (≠ M195)
4urfB Molecular genetic and crystal structural analysis of 1-(4- hydroxyphenyl)-ethanol dehydrogenase from aromatoleum aromaticum ebn1 (see paper)
44% identity, 97% coverage: 5:250/254 of query aligns to 3:244/248 of 4urfB
- active site: G16 (= G18), S142 (= S145), I152 (≠ N155), Y155 (= Y158), K159 (= K162)
- binding 3-pyridinium-1-ylpropane-1-sulfonate: L210 (≠ I216), R211 (≠ G217), R212 (= R218)
- binding bicarbonate ion: I92 (≠ V94), G94 (≠ K97), R109 (= R112), R179 (= R182), S228 (= S234)
- binding nicotinamide-adenine-dinucleotide: G12 (= G14), G14 (≠ A16), N15 (= N17), G16 (= G18), I17 (= I19), D36 (= D38), I37 (= I39), D62 (= D64), T63 (≠ V65), N89 (= N91), A90 (= A92), G91 (= G93), I140 (≠ T143), Y155 (= Y158), K159 (= K162), P185 (= P188), A186 (≠ G189), I188 (= I191), T190 (= T193)
4urfA Molecular genetic and crystal structural analysis of 1-(4- hydroxyphenyl)-ethanol dehydrogenase from aromatoleum aromaticum ebn1 (see paper)
44% identity, 97% coverage: 5:250/254 of query aligns to 3:244/248 of 4urfA
- active site: G16 (= G18), S142 (= S145), I152 (≠ N155), Y155 (= Y158), K159 (= K162)
- binding 3-pyridinium-1-ylpropane-1-sulfonate: I92 (≠ V94), S93 (≠ Q96), G94 (≠ K97), E95 (≠ Q98), T97 (≠ V100), E101 (= E104), T103 (≠ D106), Q106 (≠ E109), R109 (= R112), S175 (≠ A178), G177 (≠ N180)
- binding magnesium ion: S237 (≠ H243), Y238 (≠ A244)
- binding nicotinamide-adenine-dinucleotide: G12 (= G14), G14 (≠ A16), N15 (= N17), G16 (= G18), I17 (= I19), D36 (= D38), I37 (= I39), W41 (≠ G43), D62 (= D64), T63 (≠ V65), N89 (= N91), A90 (= A92), G91 (= G93), I140 (≠ T143), Y155 (= Y158), K159 (= K162), P185 (= P188), I188 (= I191), T190 (= T193)
4ureB Molecular genetic and crystal structural analysis of 1-(4- hydroxyphenyl)-ethanol dehydrogenase from aromatoleum aromaticum ebn1 (see paper)
44% identity, 97% coverage: 5:250/254 of query aligns to 3:244/248 of 4ureB
- active site: G16 (= G18), S142 (= S145), I152 (≠ N155), Y155 (= Y158), K159 (= K162)
- binding 3-pyridinium-1-ylpropane-1-sulfonate: N15 (= N17), G16 (= G18), I17 (= I19), N89 (= N91), G91 (= G93), Y155 (= Y158), P185 (= P188), A186 (≠ G189)
6ixmC Crystal structure of the ketone reductase chkred20 from the genome of chryseobacterium sp. Ca49 complexed with NAD (see paper)
42% identity, 98% coverage: 5:252/254 of query aligns to 3:246/248 of 6ixmC
- active site: G16 (= G18), S142 (= S145), Y155 (= Y158), K159 (= K162)
- binding nicotinamide-adenine-dinucleotide: G12 (= G14), S15 (≠ N17), G16 (= G18), I17 (= I19), D36 (= D38), I37 (= I39), A61 (≠ C63), D62 (= D64), T63 (≠ V65), N89 (= N91), A90 (= A92), M140 (≠ T143), S142 (= S145), Y155 (= Y158), K159 (= K162), P185 (= P188), A186 (≠ G189), Y187 (= Y190), I188 (= I191), L192 (≠ M195)
5itvA Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
38% identity, 99% coverage: 1:252/254 of query aligns to 1:253/255 of 5itvA
- active site: G18 (= G18), S141 (= S145), Y154 (= Y158), K158 (= K162)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G14), S17 (≠ N17), G18 (= G18), I19 (= I19), D38 (= D38), I39 (= I39), T61 (≠ C63), I63 (≠ V65), N89 (= N91), G91 (= G93), T139 (= T143), S141 (= S145), Y154 (= Y158), K158 (= K162), P184 (= P188), G185 (= G189), I186 (≠ Y190), I187 (= I191)
4fn4A Short-chain NAD(h)-dependent dehydrogenase/reductase from sulfolobus acidocaldarius (see paper)
38% identity, 99% coverage: 4:254/254 of query aligns to 4:254/254 of 4fn4A
- active site: G18 (= G18), S144 (= S145), Y157 (= Y158), K161 (= K162), S202 (≠ A204)
- binding nicotinamide-adenine-dinucleotide: G14 (= G14), S17 (≠ N17), G18 (= G18), I19 (= I19), E38 (≠ D38), L39 (≠ I39), R43 (≠ G43), A63 (≠ C63), D64 (= D64), V65 (= V65), N91 (= N91), G93 (= G93), I94 (≠ V94), T142 (= T143), S144 (= S145), Y157 (= Y158), K161 (= K162), P187 (= P188), V190 (≠ I191), T192 (= T193), N193 (≠ P194), I194 (≠ M196)
5h5xC Crystal structure of nadh bound carbonyl reductase from streptomyces coelicolor
43% identity, 98% coverage: 5:252/254 of query aligns to 11:255/257 of 5h5xC
- active site: G24 (= G18), S151 (= S145), Y164 (= Y158), K168 (= K162)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G20 (= G14), S23 (≠ N17), G24 (= G18), I25 (= I19), D44 (= D38), F45 (≠ I39), L69 (≠ C63), D70 (= D64), N97 (= N91), A98 (= A92), Y164 (= Y158), K168 (= K162), P194 (= P188), G195 (= G189), I197 (= I191), T199 (= T193)
6zzqA Crystal structure of (r)-3-hydroxybutyrate dehydrogenase from acinetobacter baumannii complexed with NAD+ and acetoacetate (see paper)
38% identity, 96% coverage: 8:252/254 of query aligns to 7:258/260 of 6zzqA
- active site: G17 (= G18), S142 (= S145), Y155 (= Y158)
- binding acetoacetic acid: Q94 (≠ E95), S142 (= S145), K152 (≠ N155), Y155 (= Y158), Q196 (≠ F199)
- binding nicotinamide-adenine-dinucleotide: G13 (= G14), S16 (≠ N17), G17 (= G18), I18 (= I19), D37 (= D38), M38 (≠ I39), D63 (= D64), V64 (= V65), N90 (= N91), A91 (= A92), G92 (= G93), M140 (≠ T143), A141 (≠ S144), S142 (= S145), Y155 (= Y158), K159 (= K162), Y187 (= Y190), V188 (≠ I191), T190 (= T193)
6zzsD Crystal structure of (r)-3-hydroxybutyrate dehydrogenase from acinetobacter baumannii complexed with NAD+ and 3-oxovalerate (see paper)
37% identity, 99% coverage: 1:252/254 of query aligns to 1:259/261 of 6zzsD
- active site: G18 (= G18), S143 (= S145), Y156 (= Y158)
- binding nicotinamide-adenine-dinucleotide: G14 (= G14), S17 (≠ N17), I19 (= I19), D38 (= D38), M39 (≠ I39), D64 (= D64), V65 (= V65), N91 (= N91), A92 (= A92), G93 (= G93), M141 (≠ T143), A142 (≠ S144), S143 (= S145), Y156 (= Y158), K160 (= K162), P186 (= P188), G187 (= G189), V189 (≠ I191), T191 (= T193), L193 (≠ M195)
- binding 3-oxidanylidenepentanoic acid: Q95 (≠ E95), S143 (= S145), N145 (≠ A147), K153 (≠ N155), Y156 (= Y158), Q197 (≠ F199)
1iy8A Crystal structure of levodione reductase (see paper)
38% identity, 98% coverage: 5:252/254 of query aligns to 2:255/258 of 1iy8A
- active site: G15 (= G18), S143 (= S145), Q153 (≠ N155), Y156 (= Y158), K160 (= K162)
- binding nicotinamide-adenine-dinucleotide: G11 (= G14), S14 (≠ N17), G15 (= G18), L16 (≠ I19), D35 (= D38), V36 (≠ I39), A62 (≠ C63), D63 (= D64), V64 (= V65), N90 (= N91), G92 (= G93), I93 (≠ V94), T141 (= T143), S143 (= S145), Y156 (= Y158), K160 (= K162), P186 (= P188), G187 (= G189), T191 (= T193), P192 (= P194), M193 (= M195)
4jroC Crystal structure of 3-oxoacyl-[acyl-carrier protein]reductase (fabg) from listeria monocytogenes in complex with NADP+
38% identity, 99% coverage: 3:253/254 of query aligns to 1:247/247 of 4jroC
- active site: G16 (= G18), S142 (= S145), Q152 (≠ N155), Y155 (= Y158), K159 (= K162)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G14), S14 (≠ A16), R15 (≠ N17), G16 (= G18), I17 (= I19), N35 (vs. gap), Y36 (≠ V37), N37 (≠ D38), G38 (≠ I39), S39 (= S40), N63 (≠ D64), V64 (= V65), N90 (= N91), A91 (= A92), I93 (≠ V94), I113 (= I116), S142 (= S145), Y155 (= Y158), K159 (= K162), P185 (= P188), I188 (= I191), T190 (= T193)
Q9LBG2 Levodione reductase; (6R)-2,2,6-trimethyl-1,4-cyclohexanedione reductase; EC 1.1.1.- from Leifsonia aquatica (Corynebacterium aquaticum) (see paper)
38% identity, 98% coverage: 5:252/254 of query aligns to 11:264/267 of Q9LBG2
- 17:42 (vs. 11:36, 42% identical) binding NAD(+)
- E103 (= E95) mutation E->A,D,N,Q: 26-fold increase in Km and a much lower enantiomeric excess of the reaction products.
4wecA Crystal structure of a short chain dehydrogenase from mycobacterium smegmatis
37% identity, 96% coverage: 8:250/254 of query aligns to 11:249/258 of 4wecA
- active site: G21 (= G18), S143 (= S145), Q154 (≠ N155), Y157 (= Y158), K161 (= K162)
- binding nicotinamide-adenine-dinucleotide: G17 (= G14), A19 (= A16), S20 (≠ N17), G21 (= G18), I22 (= I19), D41 (= D38), I42 (= I39), V61 (≠ C63), D62 (= D64), V63 (= V65), N89 (= N91), T141 (= T143), Y157 (= Y158), K161 (= K162), P187 (= P188), P189 (≠ Y190), V190 (≠ I191)
3sj7A Structure of beta-ketoacetyl-coa reductase (fabg) from staphylococcus aureus complex with NADPH (see paper)
38% identity, 96% coverage: 8:250/254 of query aligns to 2:236/239 of 3sj7A
- active site: G12 (= G18), S138 (= S145), Q148 (≠ N155), Y151 (= Y158), K155 (= K162)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G14), S10 (≠ A16), R11 (≠ N17), I13 (= I19), N31 (≠ S40), Y32 (≠ E41), A33 (≠ Q42), G34 (= G43), S35 (≠ N44), A58 (≠ C63), N59 (≠ D64), V60 (= V65), N86 (= N91), A87 (= A92), T109 (≠ I116), S138 (= S145), Y151 (= Y158), K155 (= K162), P181 (= P188), G182 (= G189)
Q9KJF1 (2S)-[(R)-hydroxy(phenyl)methyl]succinyl-CoA dehydrogenase subunit BbsD; (S,R)-2-(alpha-hydroxybenzyl)succinyl-CoA dehydrogenase subunit BbsD; EC 1.1.1.429 from Thauera aromatica (see 2 papers)
35% identity, 99% coverage: 3:254/254 of query aligns to 1:246/248 of Q9KJF1
- M1 (≠ L3) modified: Initiator methionine, Removed
- S15 (≠ N17) binding NAD(+)
- D36 (= D38) binding NAD(+)
- D62 (= D64) binding NAD(+)
- I63 (≠ V65) binding NAD(+)
- N89 (= N91) binding NAD(+)
- Y153 (= Y158) binding NAD(+)
- K157 (= K162) binding NAD(+)
3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
37% identity, 96% coverage: 8:250/254 of query aligns to 5:243/246 of 3osuA
4nbuB Crystal structure of fabg from bacillus sp (see paper)
37% identity, 97% coverage: 5:250/254 of query aligns to 5:241/244 of 4nbuB
- active site: G18 (= G18), N111 (≠ D117), S139 (= S145), Q149 (≠ N155), Y152 (= Y158), K156 (= K162)
- binding acetoacetyl-coenzyme a: D93 (≠ K97), K98 (≠ L102), S139 (= S145), N146 (≠ I152), V147 (≠ K153), Q149 (≠ N155), Y152 (= Y158), F184 (≠ Y190), M189 (= M195), K200 (≠ R209)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G14), N17 (= N17), G18 (= G18), I19 (= I19), D38 (= D38), F39 (≠ I39), V59 (≠ C63), D60 (= D64), V61 (= V65), N87 (= N91), A88 (= A92), G89 (= G93), I90 (≠ V94), T137 (= T143), S139 (= S145), Y152 (= Y158), K156 (= K162), P182 (= P188), F184 (≠ Y190), T185 (≠ I191), T187 (= T193), M189 (= M195)
1edoA The x-ray structure of beta-keto acyl carrier protein reductase from brassica napus complexed with NADP+ (see paper)
37% identity, 96% coverage: 9:253/254 of query aligns to 3:244/244 of 1edoA
- active site: G12 (= G18), S138 (= S145), Y151 (= Y158), K155 (= K162)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G14), S10 (≠ A16), R11 (≠ N17), I13 (= I19), N31 (≠ D38), Y32 (≠ I39), A33 (vs. gap), R34 (vs. gap), S35 (= S40), D59 (= D64), V60 (= V65), N86 (= N91), A87 (= A92), S138 (= S145), Y151 (= Y158), K155 (= K162), P181 (= P188), G182 (= G189), I184 (= I191), S186 (≠ T193), M188 (= M195)
Query Sequence
>WP_020563736.1 NCBI__GCF_000372865.1:WP_020563736.1
MNLSFKNKVAFVTGAANGIGRAAALAFAREGACVTVVDISEQGNQETACQIEDIGGRALA
VTCDVTRLEDVKSALDQTVGTFGRLDAAFNNAGVEQKQLVPLAELDEAEWDRIMTIDLRS
VFLCMKYEIPLMLKHGGGAIVNTSSGAGIIGIKGNPAYTAAKHGVIGLTRAAALDYAAAN
VRINAVCPGYIDTPMMARFTGGTAEGRARVISEEPIGRMGQPEEIANAVLWLCSDAVPFI
IGHALVIDGGQTIM
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory