SitesBLAST

A0QUX7 Acetolactate synthase small subunit; Acetohydroxy-acid synthase small subunit; AHAS; ALS; EC 2.2.1.6 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
45% identity, 98% coverage: 3:161/163 of query aligns to 8:166/170 of A0QUX7

query
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A0QUX7

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K46 (≠ P41) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

Q9FFF4 Acetolactate synthase small subunit 2, chloroplastic; ALS-interacting protein 3; Acetohydroxy-acid synthase small subunit 2; Protein valine-tolerant 1 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
43% identity, 98% coverage: 2:160/163 of query aligns to 76:232/477 of Q9FFF4

query
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Q9FFF4

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G88 (= G14) mutation to D: Resistance to valine inhibition.
T119 (= T47) mutation to I: In vat1-1; resistance to valine inhibition.

Sites not aligning to the query:

453 R→Q: In vat1-3; resistance to valine inhibition.

P9WKJ3 Putative acetolactate synthase small subunit; Acetohydroxy-acid synthase small subunit; AHAS; ALS; EC 2.2.1.6 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
44% identity, 98% coverage: 3:161/163 of query aligns to 6:164/168 of P9WKJ3

query
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P9WKJ3

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K44 (≠ P41) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

6vz8F Arabidopsis thaliana acetohydroxyacid synthase complex with valine bound (see paper)
41% identity, 97% coverage: 2:159/163 of query aligns to 4:158/159 of 6vz8F

query
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6vz8F

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binding valine: D13 (≠ N11), E14 (= E12), G16 (= G14), M17 (≠ A15), I18 (≠ L16), N31 (= N29), I32 (= I30)

Q93YZ7 Acetolactate synthase small subunit 1, chloroplastic; ALS-interacting protein 1; Acetohydroxyacid synthase small subunit 1 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
39% identity, 98% coverage: 2:160/163 of query aligns to 86:242/491 of Q93YZ7

query
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Q93YZ7

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D95 (≠ N11) binding L-valine
G98 (= G14) mutation to E: Increased feedback inhibition by valine/isoleucine.
M99 (≠ A15) binding L-valine
I100 (≠ L16) binding L-valine
F107 (= F23) mutation to A: Decreased feedback inhibition by leucine and valine/isoleucine.
Y112 (= Y28) mutation to A: Increased feedback inhibition by leucine.
N113 (= N29) binding L-valine; mutation to A: Decreased feedback inhibition by valine/isoleucine.
I114 (= I30) binding L-valine

Sites not aligning to the query:

328 binding L-valine
331 G→D: Decreased feedback inhibition by leucine and valine/isoleucine.
332 binding L-valine
333 binding L-valine
340 F→A: Decreased feedback inhibition by valine/isoleucine.
345 Y→A: No effect.
346 binding L-valine; mutation N->A,H: Decreased feedback inhibition by leucine and increased feedback inhibition by valine/isoleucine.
347 binding L-valine

O60086 Probable acetolactate synthase small subunit; Acetohydroxy-acid synthase small subunit; AHAS; ALS from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
30% identity, 96% coverage: 2:157/163 of query aligns to 70:265/289 of O60086

query
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O60086

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Sites not aligning to the query:

34 modified: Phosphoserine

6vz8G Arabidopsis thaliana acetohydroxyacid synthase complex with valine bound (see paper)
36% identity, 94% coverage: 3:156/163 of query aligns to 4:157/159 of 6vz8G

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binding valine: D12 (≠ N11), G15 (= G14), V16 (≠ A15), L17 (= L16), N30 (= N29), I31 (= I30), V36 (= V35)

6wo1B Hybrid acetohydroxyacid synthase complex structure with cryptococcus neoformans ahas catalytic subunit and saccharomyces cerevisiae ahas regulatory subunit (see paper)
31% identity, 95% coverage: 2:156/163 of query aligns to 4:195/197 of 6wo1B

query
sites
6wo1B

R

Q

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binding valine: E14 (= E12), G16 (= G14), L18 (= L16)

6u9dL Saccharomyces cerevisiae acetohydroxyacid synthase (see paper)
29% identity, 91% coverage: 2:150/163 of query aligns to 37:234/255 of 6u9dL

query
sites
6u9dL

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binding adenosine-5'-triphosphate: N191 (≠ I107), F192 (= F108), V197 (≠ I113), D198 (= D114), I199 (≠ V115), K211 (≠ P127), R214 (≠ K130), R214 (≠ K130)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 240

5yumA Crystallographic structures of ilvn.Val/ile complexes:conformational selectivity for feedback inhibition of ahass (see paper)
33% identity, 43% coverage: 4:73/163 of query aligns to 5:73/91 of 5yumA

query
sites
5yumA

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binding cobalt (ii) ion: H13 (≠ E12), D69 (= D69)
binding isoleucine: N12 (= N11), H13 (≠ E12), V16 (≠ A15), M17 (≠ L16), C36 (≠ V35)

5yppE Crystal structure of ilvn.Val-1a (see paper)
33% identity, 43% coverage: 4:73/163 of query aligns to 5:73/91 of 5yppE

query
sites
5yppE

I

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binding valine: H13 (≠ E12), M17 (≠ L16), N30 (= N29), V31 (≠ I30), C36 (≠ V35)

Query Sequence

>WP_020564705.1 NCBI__GCF_000372865.1:WP_020564705.1
MRHIISILMENESGALSRVAGLFSARGYNIESLTVAPTEDPTLSRMTIVTRGSDEIIEQI
TKQLNKLIDVVKLIDLAESAHIERELMLVKVRTTEQTRAEIRSMSDIFRGKIIDVTSSSY
VVEMTGPSEKLDAFIAGIDPDAIIEVVRSGPTGILRGEKGLHL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory