SitesBLAST
Comparing WP_020564706.1 NCBI__GCF_000372865.1:WP_020564706.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
6denA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide iodomuron ethyl (see paper)
44% identity, 98% coverage: 3:568/578 of query aligns to 14:581/599 of 6denA
- active site: Y35 (= Y24), G37 (= G26), G38 (= G27), A39 (= A28), I40 (≠ V29), E61 (= E50), T84 (= T73), F123 (= F112), Q124 (= Q113), E125 (= E114), K173 (= K162), K230 (≠ R223), M266 (= M259), V293 (= V286), V409 (= V394), L434 (= L419), G435 (= G420), M437 (= M422), D462 (≠ E447), N489 (= N474), E491 (≠ Y476), Q492 (≠ M477), M494 (= M479), V495 (= V480), W498 (= W483), L520 (= L506), N525 (≠ G511), V526 (≠ H512)
- binding flavin-adenine dinucleotide: R163 (= R152), G219 (= G213), A220 (≠ G214), G221 (= G215), N224 (≠ L218), T246 (= T239), L247 (= L240), Q248 (≠ M241), L264 (= L257), G286 (= G279), A287 (= A280), R288 (= R281), D290 (= D283), R292 (= R285), V293 (= V286), E319 (≠ D303), I320 (≠ V304), N324 (≠ S308), D338 (≠ E322), V339 (= V323), M414 (= M399), G432 (= G417)
- binding ethyl 2-{[(4-iodo-6-methoxypyrimidin-2-yl)carbamoyl]sulfamoyl}benzoate: M266 (= M259), D291 (= D284), R292 (= R285), W498 (= W483)
- binding magnesium ion: D462 (≠ E447), N489 (= N474), E491 (≠ Y476)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V409 (= V394), G410 (= G395), Q411 (= Q396), H412 (= H397), G435 (= G420), M437 (= M422), G461 (= G446), D462 (≠ E447), A463 (= A448), S464 (= S449), N489 (= N474), E491 (≠ Y476), Q492 (≠ M477), G493 (= G478), M494 (= M479), V495 (= V480)
6deqA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide penoxsulam (see paper)
44% identity, 98% coverage: 3:568/578 of query aligns to 14:583/601 of 6deqA
- active site: Y35 (= Y24), G37 (= G26), G38 (= G27), A39 (= A28), I40 (≠ V29), E61 (= E50), T84 (= T73), F123 (= F112), Q124 (= Q113), E125 (= E114), K173 (= K162), K232 (≠ R223), M268 (= M259), V295 (= V286), V411 (= V394), L436 (= L419), G437 (= G420), M439 (= M422), D464 (≠ E447), N491 (= N474), E493 (≠ Y476), Q494 (≠ M477), M496 (= M479), V497 (= V480), W500 (= W483), L522 (= L506), N527 (≠ G511), V528 (≠ H512)
- binding flavin-adenine dinucleotide: R163 (= R152), G221 (= G213), A222 (≠ G214), G223 (= G215), N226 (≠ L218), T248 (= T239), L249 (= L240), Q250 (≠ M241), L266 (= L257), G288 (= G279), A289 (= A280), R290 (= R281), D292 (= D283), R294 (= R285), V295 (= V286), E321 (≠ D303), I322 (≠ V304), D340 (≠ E322), V341 (= V323), M416 (= M399), G434 (= G417)
- binding magnesium ion: D464 (≠ E447), N491 (= N474), E493 (≠ Y476)
- binding 2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5-c]pyrimidin-2-yl)-6-(trifluoromethyl)benzenesulfonamide: M268 (= M259), R294 (= R285), M496 (= M479), V497 (= V480), W500 (= W483), A571 (= A556)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V411 (= V394), G412 (= G395), Q413 (= Q396), H414 (= H397), M439 (= M422), G463 (= G446), D464 (≠ E447), A465 (= A448), S466 (= S449), N491 (= N474), E493 (≠ Y476), Q494 (≠ M477), G495 (= G478), M496 (= M479), V497 (= V480)
6demA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide bensulfuron methyl (see paper)
44% identity, 98% coverage: 3:568/578 of query aligns to 12:579/597 of 6demA
- active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), I38 (≠ V29), E59 (= E50), T82 (= T73), F121 (= F112), Q122 (= Q113), E123 (= E114), K171 (= K162), K228 (≠ R223), M264 (= M259), V291 (= V286), V407 (= V394), L432 (= L419), G433 (= G420), M435 (= M422), D460 (≠ E447), N487 (= N474), E489 (≠ Y476), Q490 (≠ M477), M492 (= M479), V493 (= V480), W496 (= W483), L518 (= L506), N523 (≠ G511), V524 (≠ H512)
- binding methyl 2-[(4,6-dimethoxypyrimidin-2-yl)carbamoylsulfamoylmethyl]benzoate: M264 (= M259), D289 (= D284), R290 (= R285), M492 (= M479), W496 (= W483), A567 (= A556)
- binding flavin-adenine dinucleotide: R161 (= R152), G217 (= G213), A218 (≠ G214), G219 (= G215), N222 (≠ L218), T244 (= T239), L245 (= L240), Q246 (≠ M241), L262 (= L257), G284 (= G279), A285 (= A280), R286 (= R281), D288 (= D283), R290 (= R285), V291 (= V286), E317 (≠ D303), I318 (≠ V304), N322 (≠ S308), D336 (≠ E322), V337 (= V323), M412 (= M399), G430 (= G417)
- binding magnesium ion: D460 (≠ E447), N487 (= N474), E489 (≠ Y476)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V407 (= V394), G408 (= G395), Q409 (= Q396), H410 (= H397), M435 (= M422), G459 (= G446), D460 (≠ E447), A461 (= A448), S462 (= S449), M465 (= M452), N487 (= N474), E489 (≠ Y476), Q490 (≠ M477), G491 (= G478), M492 (= M479), V493 (= V480)
6delA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide chlorimuron ethyl (see paper)
44% identity, 98% coverage: 3:568/578 of query aligns to 12:579/597 of 6delA
- active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), I38 (≠ V29), E59 (= E50), T82 (= T73), F121 (= F112), Q122 (= Q113), E123 (= E114), K171 (= K162), K228 (≠ R223), M264 (= M259), V291 (= V286), V407 (= V394), L432 (= L419), G433 (= G420), M435 (= M422), D460 (≠ E447), N487 (= N474), E489 (≠ Y476), Q490 (≠ M477), M492 (= M479), V493 (= V480), W496 (= W483), L518 (= L506), N523 (≠ G511), V524 (≠ H512)
- binding 2-[[[[(4-chloro-6-methoxy-2-pyrimidinyl)amino]carbonyl]amino]sulfonyl]benzoic acid ethyl ester: D289 (= D284), R290 (= R285), W496 (= W483)
- binding flavin-adenine dinucleotide: R161 (= R152), G217 (= G213), A218 (≠ G214), G219 (= G215), N222 (≠ L218), T244 (= T239), L245 (= L240), Q246 (≠ M241), L262 (= L257), G284 (= G279), A285 (= A280), R286 (= R281), D288 (= D283), R290 (= R285), V291 (= V286), E317 (≠ D303), I318 (≠ V304), N322 (≠ S308), D336 (≠ E322), V337 (= V323), M412 (= M399), G430 (= G417)
- binding (3Z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl](formyl)amino}-3-sulfanylpent-3-en-1-yl trihydrogen diphosphate: V407 (= V394), G408 (= G395), Q409 (= Q396), H410 (= H397), G433 (= G420), M435 (= M422), G459 (= G446), D460 (≠ E447), A461 (= A448), S462 (= S449), M465 (= M452), N487 (= N474), E489 (≠ Y476), Q490 (≠ M477), G491 (= G478), M492 (= M479), V493 (= V480)
- binding magnesium ion: D460 (≠ E447), N487 (= N474), E489 (≠ Y476)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V407 (= V394), G408 (= G395), Q409 (= Q396), H410 (= H397), G433 (= G420), M435 (= M422), G459 (= G446), D460 (≠ E447), A461 (= A448), S462 (= S449), M465 (= M452), N487 (= N474), E489 (≠ Y476), Q490 (≠ M477), G491 (= G478), M492 (= M479), V493 (= V480)
6deoA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide iodomuron methyl (see paper)
44% identity, 98% coverage: 3:568/578 of query aligns to 10:575/593 of 6deoA
- active site: Y31 (= Y24), G33 (= G26), G34 (= G27), A35 (= A28), I36 (≠ V29), E57 (= E50), T80 (= T73), F119 (= F112), Q120 (= Q113), E121 (= E114), K169 (= K162), K224 (≠ R223), M260 (= M259), V287 (= V286), V403 (= V394), L428 (= L419), G429 (= G420), M431 (= M422), D456 (≠ E447), N483 (= N474), E485 (≠ Y476), Q486 (≠ M477), M488 (= M479), V489 (= V480), W492 (= W483), L514 (= L506), N519 (≠ G511), V520 (≠ H512)
- binding flavin-adenine dinucleotide: R159 (= R152), G213 (= G213), A214 (≠ G214), G215 (= G215), N218 (≠ L218), T240 (= T239), L241 (= L240), Q242 (≠ M241), L258 (= L257), G280 (= G279), A281 (= A280), R282 (= R281), D284 (= D283), R286 (= R285), V287 (= V286), E313 (≠ D303), I314 (≠ V304), N318 (≠ S308), D332 (≠ E322), V333 (= V323), M408 (= M399), G426 (= G417)
- binding methyl 2-{[(4-iodo-6-methoxypyrimidin-2-yl)carbamoyl]sulfamoyl}benzoate: M260 (= M259), D285 (= D284), R286 (= R285), M488 (= M479), W492 (= W483)
- binding magnesium ion: D456 (≠ E447), N483 (= N474), E485 (≠ Y476)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V403 (= V394), G404 (= G395), Q405 (= Q396), H406 (= H397), G429 (= G420), M431 (= M422), G455 (= G446), D456 (≠ E447), A457 (= A448), S458 (= S449), M461 (= M452), N483 (= N474), E485 (≠ Y476), Q486 (≠ M477), G487 (= G478), M488 (= M479), V489 (= V480)
6derA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide metosulam (see paper)
44% identity, 98% coverage: 3:568/578 of query aligns to 14:582/600 of 6derA
- active site: Y35 (= Y24), G37 (= G26), G38 (= G27), A39 (= A28), I40 (≠ V29), E61 (= E50), T84 (= T73), F123 (= F112), Q124 (= Q113), E125 (= E114), K173 (= K162), K231 (≠ R223), M267 (= M259), V294 (= V286), V410 (= V394), L435 (= L419), G436 (= G420), M438 (= M422), D463 (≠ E447), N490 (= N474), E492 (≠ Y476), Q493 (≠ M477), M495 (= M479), V496 (= V480), W499 (= W483), L521 (= L506), N526 (≠ G511), V527 (≠ H512)
- binding flavin-adenine dinucleotide: R163 (= R152), G220 (= G213), A221 (≠ G214), G222 (= G215), N225 (≠ L218), T247 (= T239), L248 (= L240), Q249 (≠ M241), L265 (= L257), H268 (= H260), G287 (= G279), A288 (= A280), R289 (= R281), D291 (= D283), R293 (= R285), V294 (= V286), E320 (≠ D303), I321 (≠ V304), N325 (≠ S308), G338 (= G321), D339 (≠ E322), V340 (= V323), Q414 (= Q398), M415 (= M399), G433 (= G417)
- binding Metosulam: R293 (= R285), M495 (= M479), W499 (= W483), A570 (= A556)
- binding magnesium ion: D463 (≠ E447), N490 (= N474), E492 (≠ Y476)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V410 (= V394), G411 (= G395), Q412 (= Q396), H413 (= H397), G436 (= G420), M438 (= M422), G462 (= G446), D463 (≠ E447), A464 (= A448), S465 (= S449), N490 (= N474), E492 (≠ Y476), Q493 (≠ M477), G494 (= G478), M495 (= M479), V496 (= V480)
- binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V410 (= V394), G411 (= G395), Q412 (= Q396), H413 (= H397), G436 (= G420), M438 (= M422), G462 (= G446), D463 (≠ E447), A464 (= A448), S465 (= S449), M468 (= M452), N490 (= N474), E492 (≠ Y476), Q493 (≠ M477), G494 (= G478), V496 (= V480)
6desA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide propoxycarbazone (see paper)
44% identity, 98% coverage: 3:568/578 of query aligns to 12:580/598 of 6desA
- active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), I38 (≠ V29), E59 (= E50), T82 (= T73), F121 (= F112), Q122 (= Q113), E123 (= E114), K171 (= K162), K229 (≠ R223), M265 (= M259), V292 (= V286), V408 (= V394), L433 (= L419), G434 (= G420), M436 (= M422), D461 (≠ E447), N488 (= N474), E490 (≠ Y476), Q491 (≠ M477), M493 (= M479), V494 (= V480), W497 (= W483), L519 (= L506), N524 (≠ G511), V525 (≠ H512)
- binding methyl 2-[(4-methyl-5-oxidanylidene-3-propoxy-1,2,4-triazol-1-yl)carbonylsulfamoyl]benzoate: M265 (= M259), D290 (= D284), R291 (= R285), W497 (= W483)
- binding flavin-adenine dinucleotide: R161 (= R152), G218 (= G213), A219 (≠ G214), G220 (= G215), N223 (≠ L218), T245 (= T239), L246 (= L240), Q247 (≠ M241), L263 (= L257), G285 (= G279), A286 (= A280), R287 (= R281), D289 (= D283), R291 (= R285), V292 (= V286), E318 (≠ D303), I319 (≠ V304), N323 (≠ S308), D337 (≠ E322), V338 (= V323), Q412 (= Q398), M413 (= M399), G431 (= G417)
- binding magnesium ion: D461 (≠ E447), N488 (= N474), E490 (≠ Y476)
- binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V408 (= V394), G409 (= G395), Q410 (= Q396), H411 (= H397), G434 (= G420), M436 (= M422), G460 (= G446), D461 (≠ E447), A462 (= A448), S463 (= S449), N488 (= N474), E490 (≠ Y476), Q491 (≠ M477), G492 (= G478), M493 (= M479), V494 (= V480)
6depA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide sulfometuron methyl (see paper)
44% identity, 98% coverage: 3:568/578 of query aligns to 12:580/598 of 6depA
- active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), I38 (≠ V29), E59 (= E50), T82 (= T73), F121 (= F112), Q122 (= Q113), E123 (= E114), K171 (= K162), K229 (≠ R223), M265 (= M259), V292 (= V286), V408 (= V394), L433 (= L419), G434 (= G420), M436 (= M422), D461 (≠ E447), N488 (= N474), E490 (≠ Y476), Q491 (≠ M477), M493 (= M479), V494 (= V480), W497 (= W483), L519 (= L506), N524 (≠ G511), V525 (≠ H512)
- binding methyl 2-[({[(4,6-dimethylpyrimidin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: D290 (= D284), R291 (= R285), M493 (= M479), W497 (= W483)
- binding flavin-adenine dinucleotide: R161 (= R152), G218 (= G213), A219 (≠ G214), G220 (= G215), N223 (≠ L218), T245 (= T239), L246 (= L240), Q247 (≠ M241), L263 (= L257), G264 (= G258), G285 (= G279), A286 (= A280), R287 (= R281), D289 (= D283), R291 (= R285), V292 (= V286), E318 (≠ D303), I319 (≠ V304), N323 (≠ S308), D337 (≠ E322), V338 (= V323), M413 (= M399), G431 (= G417)
- binding magnesium ion: D461 (≠ E447), N488 (= N474), E490 (≠ Y476)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V408 (= V394), G409 (= G395), Q410 (= Q396), H411 (= H397), G434 (= G420), M436 (= M422), G460 (= G446), D461 (≠ E447), A462 (= A448), S463 (= S449), M466 (= M452), N488 (= N474), E490 (≠ Y476), Q491 (≠ M477), G492 (= G478), M493 (= M479), V494 (= V480)
- binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V408 (= V394), G409 (= G395), Q410 (= Q396), H411 (= H397), G434 (= G420), M436 (= M422), G460 (= G446), D461 (≠ E447), A462 (= A448), S463 (= S449), M466 (= M452), N488 (= N474), E490 (≠ Y476), Q491 (≠ M477), G492 (= G478), M493 (= M479), V494 (= V480)
7egvA Acetolactate synthase from trichoderma harzianum with inhibitor harzianic acid (see paper)
45% identity, 99% coverage: 4:577/578 of query aligns to 8:581/590 of 7egvA
- active site: Y28 (= Y24), G30 (= G26), G31 (= G27), A32 (= A28), I33 (≠ V29), E54 (= E50), T77 (= T73), F116 (= F112), Q117 (= Q113), K166 (= K162), E220 (= E224), M256 (= M259), V283 (= V286), V400 (= V394), L425 (= L419), G426 (= G420), M428 (= M422), Q483 (≠ M477), M485 (= M479), V486 (= V480), W489 (= W483), L511 (= L506), G516 (= G511), I517 (≠ H512)
- binding flavin-adenine dinucleotide: R156 (= R152), G209 (= G213), Q210 (≠ G214), G211 (= G215), T236 (= T239), L237 (= L240), H238 (≠ M241), G276 (= G279), S277 (≠ A280), R278 (= R281), D280 (= D283), R282 (= R285), V283 (= V286), E309 (≠ D303), I310 (≠ V304), D328 (≠ E322), V329 (= V323), M405 (= M399), G423 (= G417), G424 (= G418)
- binding (2S)-3-methyl-2-[[(2S,4R)-1-methyl-4-[(2E,4E)-octa-2,4-dienoyl]-3,5-bis(oxidanylidene)pyrrolidin-2-yl]methyl]-2-oxidanyl-butanoic acid: F493 (≠ S487), Y494 (= Y488)
- binding magnesium ion: D453 (≠ E447), N480 (= N474), E482 (≠ Y476)
- binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: P29 (= P25), E54 (= E50), Q117 (= Q113), V400 (= V394), G401 (= G395), Q402 (= Q396), H403 (= H397), G426 (= G420), M428 (= M422), D453 (≠ E447), A454 (= A448), S455 (= S449), E482 (≠ Y476), Q483 (≠ M477), G484 (= G478), M485 (= M479), V486 (= V480)
P07342 Acetolactate synthase catalytic subunit, mitochondrial; Acetohydroxy-acid synthase catalytic subunit; AHAS; ALS; EC 2.2.1.6 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
46% identity, 96% coverage: 3:557/578 of query aligns to 92:658/687 of P07342
- R241 (= R152) binding FAD
- 355:376 (vs. 260:281, 64% identical) binding FAD
- 407:426 (vs. 303:322, 35% identical) binding FAD
1t9bB Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorsulfuron (see paper)
45% identity, 97% coverage: 3:563/578 of query aligns to 8:572/595 of 1t9bB
- active site: Y29 (= Y24), G31 (= G26), G32 (= G27), A33 (= A28), I34 (≠ V29), E55 (= E50), T78 (= T73), F117 (= F112), Q118 (= Q113), E119 (= E114), K167 (= K162), R226 (= R223), M262 (= M259), V289 (= V286), V405 (= V394), L430 (= L419), G431 (= G420), M433 (= M422), D458 (≠ E447), N485 (= N474), E487 (≠ Y476), Q488 (≠ M477), M490 (= M479), V491 (= V480), W494 (= W483), L516 (= L506), G521 (= G511), L522 (≠ H512), K555 (≠ R546)
- binding 1-(2-chlorophenylsulfonyl)-3-(4-methoxy-6-methyl-l,3,5-triazin-2-yl)urea: V107 (= V102), P108 (≠ S103), D287 (= D284), R288 (= R285), M490 (= M479), W494 (= W483)
- binding flavin-adenine dinucleotide: R157 (= R152), G215 (= G213), A216 (≠ G214), G217 (= G215), N220 (vs. gap), T242 (= T239), L243 (= L240), Q244 (≠ M241), M259 (= M256), L260 (= L257), M262 (= M259), H263 (= H260), G282 (= G279), A283 (= A280), R284 (= R281), D286 (= D283), R288 (= R285), V289 (= V286), E315 (≠ D303), V316 (= V304), N320 (≠ S308), G333 (= G321), D334 (≠ E322), A335 (≠ V323), Q409 (= Q398), M410 (= M399), G428 (= G417), G429 (= G418)
- binding magnesium ion: D458 (≠ E447), N485 (= N474), E487 (≠ Y476)
1t9cA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, sulfometuron methyl (see paper)
45% identity, 97% coverage: 3:563/578 of query aligns to 8:573/596 of 1t9cA
- active site: Y29 (= Y24), G31 (= G26), G32 (= G27), A33 (= A28), I34 (≠ V29), E55 (= E50), T78 (= T73), F117 (= F112), Q118 (= Q113), E119 (= E114), K167 (= K162), R227 (= R223), M263 (= M259), V290 (= V286), V406 (= V394), L431 (= L419), G432 (= G420), M434 (= M422), D459 (≠ E447), N486 (= N474), E488 (≠ Y476), Q489 (≠ M477), M491 (= M479), V492 (= V480), W495 (= W483), L517 (= L506), G522 (= G511), L523 (≠ H512), K556 (≠ R546)
- binding methyl 2-[({[(4,6-dimethylpyrimidin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: G32 (= G27), V107 (= V102), P108 (≠ S103), F117 (= F112), K167 (= K162), D288 (= D284), R289 (= R285), W495 (= W483)
- binding flavin-adenine dinucleotide: R157 (= R152), G216 (= G213), A217 (≠ G214), G218 (= G215), N221 (vs. gap), T243 (= T239), L244 (= L240), Q245 (≠ M241), L261 (= L257), M263 (= M259), H264 (= H260), G283 (= G279), A284 (= A280), R285 (= R281), D287 (= D283), R289 (= R285), V290 (= V286), E316 (≠ D303), V317 (= V304), N321 (≠ S308), G334 (= G321), D335 (≠ E322), A336 (≠ V323), M411 (= M399), G429 (= G417), G430 (= G418)
- binding magnesium ion: D459 (≠ E447), N486 (= N474), E488 (≠ Y476)
1t9dA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, metsulfuron methyl (see paper)
45% identity, 97% coverage: 3:563/578 of query aligns to 8:573/596 of 1t9dA
- active site: Y29 (= Y24), G31 (= G26), G32 (= G27), A33 (= A28), I34 (≠ V29), E55 (= E50), T78 (= T73), F117 (= F112), Q118 (= Q113), E119 (= E114), K167 (= K162), R227 (= R223), M263 (= M259), V290 (= V286), V406 (= V394), L431 (= L419), G432 (= G420), M434 (= M422), D459 (≠ E447), N486 (= N474), E488 (≠ Y476), Q489 (≠ M477), M491 (= M479), V492 (= V480), W495 (= W483), L517 (= L506), G522 (= G511), L523 (≠ H512), K556 (≠ R546)
- binding methyl 2-[({[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: G32 (= G27), A33 (= A28), V107 (= V102), P108 (≠ S103), F117 (= F112), K167 (= K162), M263 (= M259), D288 (= D284), R289 (= R285), W495 (= W483)
- binding flavin-adenine dinucleotide: R157 (= R152), G216 (= G213), A217 (≠ G214), G218 (= G215), N221 (vs. gap), T243 (= T239), L244 (= L240), Q245 (≠ M241), M260 (= M256), L261 (= L257), H264 (= H260), G283 (= G279), A284 (= A280), R285 (= R281), D287 (= D283), R289 (= R285), V290 (= V286), E316 (≠ D303), V317 (= V304), N321 (≠ S308), G334 (= G321), D335 (≠ E322), A336 (≠ V323), Q410 (= Q398), M411 (= M399), G429 (= G417), G430 (= G418)
- binding magnesium ion: D459 (≠ E447), N486 (= N474), E488 (≠ Y476)
- binding 2,5-dimethyl-pyrimidin-4-ylamine: E55 (= E50), P81 (= P76), Q118 (= Q113), G432 (= G420), M434 (= M422), M464 (= M452)
1t9aA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, tribenuron methyl (see paper)
45% identity, 97% coverage: 3:563/578 of query aligns to 9:574/597 of 1t9aA
- active site: Y30 (= Y24), G32 (= G26), G33 (= G27), A34 (= A28), I35 (≠ V29), E56 (= E50), T79 (= T73), F118 (= F112), Q119 (= Q113), E120 (= E114), K168 (= K162), R228 (= R223), M264 (= M259), V291 (= V286), V407 (= V394), L432 (= L419), G433 (= G420), M435 (= M422), D460 (≠ E447), N487 (= N474), E489 (≠ Y476), Q490 (≠ M477), M492 (= M479), V493 (= V480), W496 (= W483), L518 (= L506), G523 (= G511), L524 (≠ H512), K557 (≠ R546)
- binding methyl 2-[4-methoxy-6-methyl-1,3,5-trazin-2-yl(methyl)carbamoylsulfamoyl]benzoate: G33 (= G27), V108 (= V102), P109 (≠ S103), F118 (= F112), K168 (= K162), M264 (= M259), D289 (= D284), R290 (= R285), M492 (= M479), V493 (= V480), W496 (= W483)
- binding flavin-adenine dinucleotide: R158 (= R152), G217 (= G213), A218 (≠ G214), G219 (= G215), N222 (vs. gap), T244 (= T239), L245 (= L240), Q246 (≠ M241), L262 (= L257), M264 (= M259), H265 (= H260), G284 (= G279), A285 (= A280), R286 (= R281), D288 (= D283), R290 (= R285), V291 (= V286), E317 (≠ D303), V318 (= V304), N322 (≠ S308), G335 (= G321), D336 (≠ E322), A337 (≠ V323), Q411 (= Q398), M412 (= M399), G430 (= G417), G431 (= G418)
- binding magnesium ion: D460 (≠ E447), N487 (= N474), E489 (≠ Y476)
- binding propyl trihydrogen diphosphate: V407 (= V394), G408 (= G395), Q409 (= Q396), H410 (= H397), M435 (= M422), G459 (= G446), D460 (≠ E447), A461 (= A448), S462 (= S449), N487 (= N474), E489 (≠ Y476), Q490 (≠ M477), G491 (= G478), M492 (= M479)
- binding 5-{[ethyl(methyl)amino]methyl}-2-methyl-5,6-dihydropyrimidin-4-amine: G433 (= G420), M435 (= M422), M465 (= M452)
6u9dB Saccharomyces cerevisiae acetohydroxyacid synthase (see paper)
45% identity, 97% coverage: 3:563/578 of query aligns to 12:584/607 of 6u9dB
- active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), I38 (≠ V29), E59 (= E50), T82 (= T73), F121 (= F112), Q122 (= Q113), E123 (= E114), K171 (= K162), M274 (= M259), V301 (= V286), V417 (= V394), G443 (= G420), M445 (= M422), D470 (≠ E447), N497 (= N474), E499 (≠ Y476), Q500 (≠ M477), M502 (= M479), V503 (= V480), W506 (= W483)
- binding methyl 2-[(4,6-dimethoxypyrimidin-2-yl)carbamoylsulfamoylmethyl]benzoate: G36 (= G27), V111 (= V102), P112 (≠ S103), F121 (= F112), K171 (= K162), D299 (= D284), R300 (= R285), M502 (= M479), W506 (= W483)
- binding flavin-adenine dinucleotide: R161 (= R152), A228 (≠ G214), G229 (= G215), N232 (vs. gap), T254 (= T239), L255 (= L240), Q256 (≠ M241), L272 (= L257), M274 (= M259), G294 (= G279), R296 (= R281), D298 (= D283), R300 (= R285), V301 (= V286), E327 (≠ D303), V328 (= V304), N332 (≠ S308), D346 (≠ E322), A347 (≠ V323), M422 (= M399), G440 (= G417), G441 (= G418)
- binding magnesium ion: D470 (≠ E447), N497 (= N474)
- binding thiamine diphosphate: E59 (= E50), P85 (= P76), V417 (= V394), G418 (= G395), Q419 (= Q396), H420 (= H397), G443 (= G420), M445 (= M422), A471 (= A448), S472 (= S449), N497 (= N474), E499 (≠ Y476), Q500 (≠ M477), G501 (= G478), M502 (= M479), V503 (= V480)
1n0hA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorimuron ethyl (see paper)
45% identity, 97% coverage: 3:563/578 of query aligns to 10:576/599 of 1n0hA
- active site: Y31 (= Y24), G33 (= G26), G34 (= G27), A35 (= A28), I36 (≠ V29), E57 (= E50), T80 (= T73), F119 (= F112), Q120 (= Q113), E121 (= E114), K169 (= K162), R230 (= R223), M266 (= M259), V293 (= V286), V409 (= V394), L434 (= L419), G435 (= G420), M437 (= M422), D462 (≠ E447), N489 (= N474), E491 (≠ Y476), Q492 (≠ M477), M494 (= M479), V495 (= V480), W498 (= W483), L520 (= L506), G525 (= G511), L526 (≠ H512), K559 (≠ R546)
- binding 4-{[(4'-amino-2'-methylpyrimidin-5'-yl)methyl]amino}pent-3-enyl diphosphate: V409 (= V394), G410 (= G395), Q411 (= Q396), H412 (= H397), G435 (= G420), M437 (= M422), G461 (= G446), D462 (≠ E447), A463 (= A448), S464 (= S449), M467 (= M452), N489 (= N474), E491 (≠ Y476), Q492 (≠ M477), G493 (= G478), V495 (= V480)
- binding 2-[[[[(4-chloro-6-methoxy-2-pyrimidinyl)amino]carbonyl]amino]sulfonyl]benzoic acid ethyl ester: G34 (= G27), A35 (= A28), V109 (= V102), P110 (≠ S103), F119 (= F112), K169 (= K162), M266 (= M259), D291 (= D284), R292 (= R285), V495 (= V480), W498 (= W483)
- binding flavin-adenine dinucleotide: R159 (= R152), G219 (= G213), A220 (≠ G214), G221 (= G215), N224 (vs. gap), T246 (= T239), L247 (= L240), Q248 (≠ M241), L264 (= L257), G265 (= G258), M266 (= M259), H267 (= H260), G286 (= G279), A287 (= A280), R288 (= R281), D290 (= D283), R292 (= R285), V293 (= V286), E319 (≠ D303), V320 (= V304), N324 (≠ S308), G337 (= G321), D338 (≠ E322), A339 (≠ V323), M414 (= M399), G432 (= G417), G433 (= G418)
- binding magnesium ion: D462 (≠ E447), N489 (= N474), E491 (≠ Y476)
- binding thiamine diphosphate: Y31 (= Y24), E57 (= E50), P83 (= P76)
7tzzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase p197t mutant in complex with bispyribac-sodium (see paper)
44% identity, 97% coverage: 5:563/578 of query aligns to 14:575/582 of 7tzzA
- binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: M266 (= M259), R292 (= R285), W489 (= W483), S568 (≠ A556)
- binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V394), G401 (= G395), Q402 (= Q396), H403 (= H397), G426 (= G420), M428 (= M422), G452 (= G446), D453 (≠ E447), G454 (≠ A448), S455 (= S449), L483 (≠ M477), G484 (= G478), M485 (= M479), V486 (= V480)
- binding flavin-adenine dinucleotide: R161 (= R152), G222 (= G213), G223 (= G214), G224 (= G215), T246 (= T239), L247 (= L240), M248 (= M241), M263 (= M256), L264 (= L257), M266 (= M259), H267 (= H260), G286 (= G279), R288 (= R281), V293 (= V286), D310 (= D303), I311 (≠ V304), D329 (≠ E322), V330 (= V323), M405 (= M399), G423 (= G417)
- binding magnesium ion: A37 (= A28), T82 (= T73), S83 (= S74), Q122 (= Q113), Y381 (= Y375), D453 (≠ E447), M458 (= M452), Q461 (= Q455), N480 (= N474), H482 (≠ Y476), K533 (≠ P520)
P17597 Acetolactate synthase, chloroplastic; AtALS; Acetohydroxy-acid synthase; Protein CHLORSULFURON RESISTANT 1; EC 2.2.1.6 from Arabidopsis thaliana (Mouse-ear cress) (see 8 papers)
44% identity, 97% coverage: 5:563/578 of query aligns to 99:660/670 of P17597
- A122 (= A28) mutation to V: Reduced catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
- M124 (≠ L30) mutation to E: Reduced catalytic activity. Resistant to imidazolinone herbicides and reduced sensitivity to sulfonylurea herbicides.; mutation to I: No effect on catalytic activity. Increased resistance to imidazolinone herbicides.
- E144 (= E50) binding thiamine diphosphate
- S186 (= S92) binding FAD
- P197 (≠ S103) mutation to S: In csr1-1/GH50; resistant to sulfonylurea but not to imidazolinone herbicides.
- R199 (≠ P105) mutation R->A,E: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
- Q207 (= Q113) binding thiamine diphosphate
- K220 (= K126) binding (R)-imazaquin
- R246 (= R152) binding (R)-imazaquin; binding FAD
- K256 (= K162) binding chlorimuron-ethyl
- G308 (= G214) binding FAD
- TL 331:332 (= TL 239:240) binding FAD
- C340 (≠ G248) modified: Cysteine sulfinic acid (-SO2H)
- LGMH 349:352 (= LGMH 257:260) binding FAD
- GVRFD 371:375 (≠ GARFD 279:283) binding FAD
- DR 376:377 (= DR 284:285) binding chlorimuron-ethyl
- DI 395:396 (≠ DV 303:304) binding FAD
- DV 414:415 (≠ EV 322:323) binding FAD
- QH 487:488 (= QH 396:397) binding thiamine diphosphate
- GG 508:509 (= GG 417:418) binding FAD
- GAM 511:513 (≠ GTM 420:422) binding thiamine diphosphate
- D538 (≠ E447) binding Mg(2+)
- DGS 538:540 (≠ EAS 447:449) binding thiamine diphosphate
- N565 (= N474) binding Mg(2+)
- NQHLGM 565:570 (≠ NRYMGM 474:479) binding thiamine diphosphate
- H567 (≠ Y476) binding Mg(2+)
- W574 (= W483) binding chlorimuron-ethyl; mutation to L: Increased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.; mutation to S: Slightly decreased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.
- S653 (≠ A556) binding chlorimuron-ethyl; mutation to A: No effect on catalytic activity or sensitivity to herbicides.; mutation to F: No effect on catalytic activity. Resistant to imidazolinone herbicides and also slightly sulfonylurea-resistant.; mutation to N: In csr1-2/GH90; no effect on catalytic activity. Resistant to imidazolinone but not to sulfonylurea herbicides.; mutation to T: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
5k2oA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, pyrithiobac (see paper)
44% identity, 97% coverage: 5:563/578 of query aligns to 14:575/585 of 5k2oA
- active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ V29), E59 (= E50), T82 (= T73), F121 (= F112), Q122 (= Q113), E123 (= E114), K171 (= K162), M266 (= M259), V293 (= V286), V400 (= V394), G426 (= G420), M428 (= M422), D453 (≠ E447), N480 (= N474), H482 (≠ Y476), L483 (≠ M477), M485 (= M479), V486 (= V480), W489 (= W483), H558 (≠ R546)
- binding 2-chloranyl-6-(4,6-dimethoxypyrimidin-2-yl)sulfanyl-benzoic acid: M266 (= M259), R292 (= R285), W489 (= W483), S568 (≠ A556)
- binding flavin-adenine dinucleotide: R161 (= R152), G222 (= G213), G223 (= G214), G224 (= G215), T246 (= T239), L247 (= L240), M248 (= M241), L264 (= L257), G286 (= G279), R288 (= R281), D290 (= D283), V293 (= V286), D310 (= D303), I311 (≠ V304), D329 (≠ E322), V330 (= V323), Q404 (= Q398), M405 (= M399), G423 (= G417)
- binding magnesium ion: D453 (≠ E447), N480 (= N474), H482 (≠ Y476)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V394), G401 (= G395), Q402 (= Q396), H403 (= H397), M428 (= M422), D453 (≠ E447), G454 (≠ A448), S455 (= S449), N480 (= N474), H482 (≠ Y476), L483 (≠ M477), G484 (= G478), M485 (= M479), V486 (= V480)
5k3sA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, bispyribac-sodium (see paper)
44% identity, 97% coverage: 5:563/578 of query aligns to 14:575/583 of 5k3sA
- active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ V29), E59 (= E50), T82 (= T73), F121 (= F112), Q122 (= Q113), E123 (= E114), K171 (= K162), M266 (= M259), V293 (= V286), V400 (= V394), G426 (= G420), M428 (= M422), D453 (≠ E447), N480 (= N474), H482 (≠ Y476), L483 (≠ M477), M485 (= M479), V486 (= V480), W489 (= W483), H558 (≠ R546)
- binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: R292 (= R285), M485 (= M479), W489 (= W483), G569 (= G557)
- binding flavin-adenine dinucleotide: R161 (= R152), G222 (= G213), G223 (= G214), G224 (= G215), T246 (= T239), L247 (= L240), M248 (= M241), L264 (= L257), M266 (= M259), G286 (= G279), R288 (= R281), D290 (= D283), V293 (= V286), D310 (= D303), I311 (≠ V304), D329 (≠ E322), V330 (= V323), M405 (= M399), G423 (= G417)
- binding magnesium ion: D453 (≠ E447), N480 (= N474), H482 (≠ Y476)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V394), G401 (= G395), Q402 (= Q396), H403 (= H397), G426 (= G420), M428 (= M422), D453 (≠ E447), G454 (≠ A448), S455 (= S449), N480 (= N474), H482 (≠ Y476), L483 (≠ M477), G484 (= G478), M485 (= M479), V486 (= V480)
Query Sequence
>WP_020564706.1 NCBI__GCF_000372865.1:WP_020564706.1
MELSGAQILVQSLKDEGVEYIFGYPGGAVLHLYDALFQQEAIKHILVRHEQGATHAADGY
ARATGKPGVVLVTSGPGATNAITGIATAYMDSIPLVVISGQVSLPVIGSDAFQEVDMIGI
SRPCVKHNFLVKDVTKLAETIKKAFYVATSGRPGPVVVDVPKDITDPNIKVPYKYPKKIS
LRSYNPVVLGHKGQIKKAVDLLLSAERPIIYSGGGVILGEASRELTEFGRLLEYPVTNTL
MGLGGYPGTDRQFVGMLGMHGTYEANMAMHESDLIIAVGARFDDRVTGKLDKFCPYARII
HIDVDPASISKTVKVDIPIVGEVKPVLEQMIELIRESKKKPNKKALEAWWSRINQWKSVN
CLDYDRESRLIKPQYVIEQLYDVTKGEAYVTSDVGQHQMWAAQYYRFDKPRHWINSGGLG
TMGFGLPAAIGVKLAFPEADVACVTGEASIQMCIQELSTALQYNTAVKIVNLNNRYMGMV
RQWQEFSYESRYSHSYMDTIPDFVKLAEAYGHVGMRIDKPEDVRGALEQAFALKDRTVFI
DIMTDRTENVYPMIEAGKAHNDMKLRNAFGSSADRELA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory