SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_020564808.1 NCBI__GCF_000372865.1:WP_020564808.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 10 hits to proteins with known functional sites (download)

3tdtA Complex of tetrahydrodipicolinate n-succinyltransferase with 2-amino- 6-oxopimelate and coenzyme a (see paper)
76% identity, 100% coverage: 1:271/272 of query aligns to 1:271/274 of 3tdtA

query
sites
3tdtA

M

M

K

Q

K

Q

L

L

E

Q

D

N

I

V

I

I

N

E

A

S

A

A

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M

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H

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G

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N

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L

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P

S

S

A

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D

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G

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C

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active site: D141 (= D141), G166 (= G166), E189 (= E189)
binding 2-amino-6-oxopimelic acid: R104 (= R104), M124 (= M124), M139 (= M139), D141 (= D141)
binding coenzyme a: G185 (= G185), A186 (= A186), S203 (= S203), M204 (= M204), R217 (= R217), V234 (= V234), K254 (= K254), K259 (= K259), T260 (= T260), K263 (= K263)

2tdtA Complex of tetrahydrodipicolinate n-succinyltransferase with 2- aminopimelate and coenzyme a (see paper)
76% identity, 100% coverage: 1:271/272 of query aligns to 1:271/274 of 2tdtA

query
sites
2tdtA

M

M

K

Q

K

Q

L

L

E

Q

D

N

I

V

I

I

N

E

A

S

A

A

F

F

E

E

Q

R

R

R

A

A

D

D

I

I

T

T

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P

A

A

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N

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V

S

D

Q

T

E

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I

T

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R

G

E

A

A

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Q

N

E

Q

C

V

L

I

N

G

L

L

L

L

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D

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S

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G

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A

A

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R

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E

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K

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W

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L

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K

A

A

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L

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F

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D

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G

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N

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L

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D

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L

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R

active site: D141 (= D141), G166 (= G166), E189 (= E189)
binding coenzyme a: S159 (= S159), F183 (= F183), G185 (= G185), S203 (= S203), M204 (= M204), R217 (= R217), K254 (= K254), K259 (= K259), T260 (= T260), K263 (= K263)
binding (2S)-2-aminoheptanedioic acid: R104 (= R104), M124 (= M124), M139 (= M139), D141 (= D141)

1kgtA Crystal structure of tetrahydrodipicolinate n-succinyltransferase in complex with pimelate and succinyl-coa (see paper)
76% identity, 100% coverage: 1:271/272 of query aligns to 1:271/274 of 1kgtA

query
sites
1kgtA

M

M

K

Q

K

Q

L

L

E

Q

D

N

I

V

I

I

N

E

A

S

A

A

F

F

E

E

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R

R

A

A

D

D

I

I

T

T

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P

A

A

N

N

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D

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T

E

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R

G

E

A

A

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N

E

Q

C

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L

I

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G

L

L

L

L

D

D

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S

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G

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A

A

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R

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A

A

E

E

K

K

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I

D

D

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G

D

Q

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T

H

H

Q

Q

W

W

L

L

K

K

K

K

A

A

V

V

L

L

L

L

S

S

F

F

R

R

I

I

N

N

E

D

N

N

R

K

L

V

M

M

D

D

G

G

G

A

N

E

T

T

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R

Y

Y

Y

Y

D

D

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K

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K

F

F

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D

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W

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K

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A

A

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G

N

N

L

L

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P

S

S

A

K

D

D

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L

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Y

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C

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A

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K

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V

D

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A

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T

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G

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N

N

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L

L

L

L

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R

active site: D141 (= D141), G166 (= G166), E189 (= E189)
binding pimelic acid: R104 (= R104), M124 (= M124), M139 (= M139)
binding succinyl-coenzyme a: D141 (= D141), S159 (= S159), A186 (= A186), S203 (= S203), R217 (= R217), K259 (= K259), T260 (= T260), K263 (= K263)

1kgqA Crystal structure of tetrahydrodipicolinate n-succinyltransferase in complex with l-2-aminopimelate and succinamide-coa (see paper)
76% identity, 100% coverage: 1:271/272 of query aligns to 1:271/274 of 1kgqA

query
sites
1kgqA

M

M

K

Q

K

Q

L

L

E

Q

D

N

I

V

I

I

N

E

A

S

A

A

F

F

E

E

Q

R

R

R

A

A

D

D

I

I

T

T

P

P

A

A

N

N

V

V

S

D

Q

T

E

V

I

T

R

R

G

E

A

A

V

V

Q

N

E

Q

C

V

L

I

N

G

L

L

L

L

D

D

S

S

G

G

K

A

A

L

R

R

V

V

A

A

E

E

K

K

I

I

D

D

G

G

D

Q

W

W

V

V

T

T

H

H

Q

Q

W

W

L

L

K

K

K

K

A

A

V

V

L

L

L

L

S

S

F

F

R

R

I

I

N

N

E

D

N

N

R

K

L

V

M

M

D

D

G

G

G

A

N

E

T

T

R

R

Y

Y

Y

Y

D

D

K

K

V

V

E

P

C

M

K

K

F

F

A

A

S

D

Y

Y

S

D

E

E

E

A

D

R

F

F

A

Q

K

K

A

E

G

G

V

F

R
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R

V

V

V

V

P

P

N

P

A

A

V

T

A

V

R

R

R

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G

G

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Y

F

I

I

A

A

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R

G

N

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L

L

M
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M

P

P

S

S

Y

Y

V

V

N

N

I

I

G

G

A

A

Y

Y

V

V

D

D

S

E

G

G

T

T

M
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M

V

V

D
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D

T

T

W

W

V

A

T

T

V

V

G

G

S

S

C

C

A

A

Q

Q

I

I

G

G

K

K

N

N

V

V

H

H

L

L

S

S

G

G

G

G

V

V

G

G

I

I

G

G

G
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G

V

V

L

L

E

E

P

P

L

L

Q

Q

A

A

G

N

P

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T

T

I

I

I

I

G

E

D

D

N

N

C

C

F
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F

I

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G
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G

A

A

R

R

S

S

E
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E

I

V

V

V

E

E

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G

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V

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I

V

V

E

E

D

E

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G

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S

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M

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V

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Y

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G

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Q

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T

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R

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Y

N

D

R
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R

M

E

T

T

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G

E

E

I

I

T

H

Y

Y

G

G

R

R

I

V

P

P

A

A

G

G

S

S

V

V

V

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V
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V

S

S

G

G

N

N

L

L

P

P

S

S

A

K

D

D

G

G

K

S

Y

Y

S

S

L

L

Y

Y

C

C

A

A

V

V

I

I

I

V

K
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K

Q

K

V

V

D

D

E

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K

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T

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R

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L

L

L

R

R

active site: D141 (= D141), G166 (= G166), E189 (= E189)
binding (2S)-2-aminoheptanedioic acid: R104 (= R104), M124 (= M124), M139 (= M139), D141 (= D141)
binding succinamide-coa: F183 (= F183), G185 (= G185), S203 (= S203), M204 (= M204), R217 (= R217), V234 (= V234), K254 (= K254), K259 (= K259), T260 (= T260), K263 (= K263)

3r8yA Structure of the bacillus anthracis tetrahydropicolinate succinyltransferase
37% identity, 36% coverage: 97:194/272 of query aligns to 70:168/203 of 3r8yA

query
sites
3r8yA

D

D

F

L

A

K

K

G

A

I

G

K

V

A

R

R

V

I

V

E

P

P

N

G

A

A

V

I

A

I

R

R

R

D

G

H

S

V

Y

E

I

I

A

G

P

D

G

N

A

A

-

V

I

I

L

M

M

M

P

N

S

A

Y

T

V

I

N

N

I

I

G

G

A

A

Y

V

V

I

D

G

S

E

G

G

T

S

M

M

V

I

D
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D

T

M

W

N

V

A

T

V

V

L

G
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G

S
x
G

C

R

A

A

Q

T

I

V

G

G

K

K

N

N

V

C

H

H

L

V

S

G

G

A

G

G

V

A

G

V

I

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G

A

G
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G

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V

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I

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E

P

P

L

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S

A

A

G

K

P

P

T

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I

I

I

V

G

E

D

D

N

D

C

V

F

V

I

I

G

G

A

A

R

N

S

V

E
x
V

I

V

V

L

E

E

G

G

V

V

active site: D115 (= D141), G140 (= G166), V163 (≠ E189)
binding calcium ion: G121 (= G147), G122 (≠ S148)

3r5bA Pseudomonas aeruginosa dapd (pa3666) in complex with l-2-aminopimelate (see paper)
31% identity, 45% coverage: 93:214/272 of query aligns to 163:284/321 of 3r5bA

query
sites
3r5bA

Y

F

S

S

E

V

E

D

D

K

F

F

A

P

K

K

-

M

-

T

-

D

-

Y

-

V

-

V

-

P

A

A

G

G

V

V

R

R

V

I

V

A

P

D

N

T

A

A

V

R

A

V

R
|
R

R

L

G

G

S

A

Y

Y

I

I

A

G

P

E

G

G

A

T

I

T

L

V

M

M

P

H

S

E

-

G

Y

F

V

V

N

N

I

F

G

N

A

A

Y

G

V

T

D

E

S

G

G

P

T

G

M

M

V

I

D

E

T

G

W

R

V

V

T
x
S

V

A

G

G

S

V

C

F

A

-

Q

-

I

V

G

G

K

K

N

G

V

S

H

D

L

L

S

G

G

G

G

G

V

C

G

S

I

T

G

M

G

G

V

T

L

L

E

N

P

I

L

V

Q

I

A

S

G

-

P

-

T

-

I

-

I

V

G

G

D

E

N

G

C

C

F

L

I

I

G

G

A

A

R

N

S

A

E

G

I

I

V

-

E

-

G

G

V

I

V

P

V

L

E

G

D

D

G

R

C

N

V

I

I

V

S

E

M

A

G

G

V

L

Y

Y

I

I

G

T

Q

A

S

G

T

T

K

K

I

V

binding (2S)-2-aminoheptanedioic acid: R189 (= R112), S223 (≠ T145)

Sites not aligning to the query:

binding (2S)-2-aminoheptanedioic acid: 127

3r5aA Pseudomonas aeruginosa dapd (pa3666) in complex with d-2-aminopimelate (see paper)
31% identity, 45% coverage: 93:214/272 of query aligns to 163:284/321 of 3r5aA

query
sites
3r5aA

Y

F

S

S

E

V

E

D

D

K

F

F

A

P

K

K

-

M

-

T

-

D

-

Y

-

V

-

V

-

P

A

A

G

G

V

V

R
|
R

V

I

V

A

P

D

N

T

A

A

V

R

A

V

R
|
R

R

L

G

G

S

A

Y

Y

I

I

A

G

P

E

G

G

A

T

I

T

L

V

M
|
M

P

H

S

E

-

G

Y

F

V

V

N
|
N

I

F

G

N

A

A

Y

G

V

T

D

E

S

G

G

P

T

G

M
|
M

V

I

D

E

T

G

W

R

V

V

T
x
S

V
x
A

G

G

S

V

C

F

A

-

Q

-

I

V

G

G

K

K

N

G

V

S

H

D

L

L

S

G

G

G

G

G

V

C

G

S

I

T

G

M

G

G

V

T

L

L

E

N

P

I

L

V

Q

I

A

S

G

-

P

-

T

-

I

-

I

V

G

G

D

E

N

G

C

C

F

L

I

I

G

G

A

A

R

N

S

A

E

G

I

I

V

-

E

-

G

G

V

I

V

P

V

L

E

G

D

D

G

R

C

N

V

I

I

V

S

E

M

A

G

G

V

L

Y

Y

I

I

G

T

Q

A

S

G

T

T

K

K

I

V

binding (2R)-2-aminoheptanedioic acid: R181 (= R104), R189 (= R112), M201 (= M124), N207 (= N129), M217 (= M139), S223 (≠ T145), A224 (≠ V146)

Sites not aligning to the query:

binding (2R)-2-aminoheptanedioic acid: 127

5e3rA Crystal structure of dapd in complex with 2-aminopimelate from corynebacterium glutamicum (see paper)
31% identity, 45% coverage: 93:214/272 of query aligns to 124:241/277 of 5e3rA

query
sites
5e3rA

Y

Y

S

S

E

V

E

D

D

K

F

F

A

P

K

R

A

M

G

V

V

D

R

Y

V

V

V

V

P

P

N

S

A

G

V

V

-
x
R

-

I

-

G

-

D

-

A

-

D

-

R

A

V

R

R

R

L

G

G

S

A

Y

Y

I

L

A

A

P

D

G

G

A

T

I

T

L

V

M

M

-

H

P

E

S

G

Y

F

V

V

N

N

I

F

G

N

A

A

Y

G

V

T

D

L

S

G

G

A

T

S

M

M

V

V

D
x
E

T

G

W

R

V

I

T

S

V

A

G

G

S

V

C

T

A

V

Q

D

I

D

G

G

K

T

N

D

V

V

H

-

L

-

S

G

G

G

G

G

V

A

G

S

I

I

G

M

G

G

V

V

L

I

E

S

P

-

L

-

Q

-

A

-

G

-

P

-

T

-

I

-

I

L

G

G

D

K

N

R

C

C

F

L

I

L

G

G

A

A

R

N

S

S

E

G

I

C

V

-

E

-

G

G

V

I

V

P

V

L

E

G

D

D

G

D

C

C

V

I

I

I

S

E

M

A

G

G

V

L

Y

Y

I

I

G

T

Q

A

S

G

T

T

K

K

I

V

binding (2S)-2-aminoheptanedioic acid: R142 (vs. gap), E180 (≠ D141)

5e3qA Crystal structure of dapd in complex with succinyl-coa from corynebacterium glutamicum (see paper)
31% identity, 45% coverage: 93:214/272 of query aligns to 124:241/278 of 5e3qA

query
sites
5e3qA

Y

Y

S

S

E

V

E

D

D

K

F

F

A

P

K

R

A

M

G

V

V

D

R

Y

V

V

V

V

P

P

N

S

A

G

V

V

-

R

-

I

-

G

-

D

-

A

-

D

-

R

A

V

R

R

R

L

G

G

S

A

Y

Y

I

L

A

A

P

D

G

G

A

T

I

T

L

V

M

M

P

H

S

E

-

G

Y

F

V

V

N

N

I

F

G

N

A

A

Y

G

V

T

D

L

S

G

G

A

T

S

M

M

V

V

D

E

T

G

W

R

V

I

T

S

V

A

G

G

S

V

C

T

A

V

Q

D

I

D

G

G

K

T

N

D

V

V

H

-

L

-

S

G

G
|
G

G

G

V

A

G

S

I

I

G

M

G

G

V

V

L

I

E

S

P

-

L

-

Q

-

A

-

G

-

P

-

T

-

I

-

I

L

G

G

D

K

N

R

C

C

F

L

I

L

G
|
G

A
|
A

R

N

S

S

E

G

I

C

V

-

E

-

G

G

V

I

V

P

V

L

E

G

D

D

G

D

C

C

V

I

I

I

S
x
E

M

A

G

G

V

L

Y

Y

I

I

G

T

Q

A

S

G

T

T

K

K

I

V

binding succinyl-coenzyme a: G197 (= G160), G214 (= G185), A215 (= A186), E230 (≠ S203)

Sites not aligning to the query:

binding succinyl-coenzyme a: 262, 263, 264, 265

3r5cA Pseudomonas aeruginosa dapd (pa3666) in complex with coa and succinate (see paper)
30% identity, 45% coverage: 93:214/272 of query aligns to 166:285/338 of 3r5cA

query
sites
3r5cA

Y

F

S

S

E

V

E

D

D

K

F

F

A

P

K

K

-

M

-

T

-

D

-

Y

-

V

-

V

-

P

A

A

G

G

V

V

R

R

V

I

V

A

P

D

N

T

A

A

V

R

A

V

R

R

R

L

G

G

S

A

Y

Y

I

I

A

G

P

E

G

G

A

T

I

T

L

V

M

M

P

H

S

E

-

G

Y

F

V

V

N

N

I

F

G

N

A

A

Y

G

V

T

D

E

S

G

G

P

T

G

M

M

V

I

D

E

T

G

W

R

V

V

T

S

V

A

G

G

S

V

C

F

A

-

Q

-

I

V

G

G

K

K

N

G

V

S

H

D

L

L

S

G

G

G

G

G

V

C

G

S

I

T

G
x
M

G

G

V

T

L

I

E

V

P

-

L

-

Q

-

A

-

G

-

P

-

T

I

I

S

I

V

G

G

D

E

N

G

C

C

F

L

I

I

G
|
G

A
|
A

R

N

S

A

E

G

I

I

V

-

E

-

G

G

V

I

V

P

V

L

E

G

D

D

G

R

C

N

V

I

I

V

S
x
E

M
x
A

G

G

V

L

Y

Y

I

I

G

T

Q

A

S
x
G

T

T

K
|
K

I

V

binding coenzyme a: M244 (≠ G165), G258 (= G185), A259 (= A186), E274 (≠ S203), A275 (≠ M204), G282 (≠ S211), K284 (= K213)

Sites not aligning to the query:

binding coenzyme a: 299, 312, 313, 314, 315

Query Sequence

>WP_020564808.1 NCBI__GCF_000372865.1:WP_020564808.1
MKKLEDIINAAFEQRADITPANVSQEIRGAVQECLNLLDSGKARVAEKIDGDWVTHQWLK
KAVLLSFRINENRLMDGGNTRYYDKVECKFASYSEEDFAKAGVRVVPNAVARRGSYIAPG
AILMPSYVNIGAYVDSGTMVDTWVTVGSCAQIGKNVHLSGGVGIGGVLEPLQAGPTIIGD
NCFIGARSEIVEGVVVEDGCVISMGVYIGQSTKIYNRMTGEITYGRIPAGSVVVSGNLPS
ADGKYSLYCAVIIKQVDEKTRSKTGINELLRD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory