SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_020565375.1 NCBI__GCF_000372865.1:WP_020565375.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P07003 Pyruvate dehydrogenase [ubiquinone]; Pyruvate oxidase; POX; Pyruvate:ubiquinone-8 oxidoreductase; EC 1.2.5.1 from Escherichia coli (strain K12) (see 4 papers)
34% identity, 98% coverage: 1:576/588 of query aligns to 1:562/572 of P07003

query
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P07003

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1:182 (vs. 1:191, 38% identical) Pyr domain
E50 (= E51) binding thiamine diphosphate
183:334 (vs. 192:343, 38% identical) FAD-binding domain
S210 (≠ A217) binding FAD
LR 234:235 (≠ LL 241:242) binding FAD
TGLI 251:254 (≠ IGLL 258:261) binding FAD
TQFPY 274:278 (≠ SSFPY 281:285) binding FAD
D292 (= D301) binding FAD
S297 (≠ L306) binding FAD
DI 311:312 (≠ DS 320:321) binding FAD
335:530 (vs. 344:548, 28% identical) PP-binding domain
T382 (≠ S391) binding thiamine diphosphate
FN 403:404 (≠ LS 410:411) binding FAD
G--SM 406:408 (≠ GLATM 413:417) binding thiamine diphosphate
D433 (= D442) binding Mg(2+)
DGG 433:435 (≠ DGA 442:444) binding thiamine diphosphate
N460 (= N475) binding Mg(2+)
460:466 (vs. 475:481, 43% identical) binding thiamine diphosphate
V462 (≠ D477) binding Mg(2+)
F465 (≠ Q480) Moves into active site upon enzyme activation, plays a role in electron transfer
A533 (≠ P551) mutation to T: In poxB11; poor activity in vivo, no longer activated by lipids.
YM 549:550 (vs. gap) In vitro cleavage to yield alpha-peptide
A553 (= A567) mutation to V: In poxB14; poor activity in vivo, no longer activated by lipids.
D560 (= D574) mutation to P: In poxB15; normal activity.

Sites not aligning to the query:

531:572 Membrane-binding domain
549:572 mutation Missing: In poxB6. Inactive in vivo, does not complement inactive mutants. Active in vitro, no longer activated by nor binds to, detergents.
564 E→P: In poxB16; loss of activity, weakly activated by cleavage.
564:572 mutation Missing: In poxB7 Inactive in vivo, reduced activity in vitro.
570:572 mutation Missing: In poxB8; reduced activity in vitro, not activated by lipids.
572 R→G: In poxB10; reduced activity in vivo and in vitro; may interact less with membranes.

3ey9A Structural basis for membrane binding and catalytic activation of the peripheral membrane enzyme pyruvate oxidase from escherichia coli (see paper)
34% identity, 98% coverage: 2:576/588 of query aligns to 1:561/571 of 3ey9A

query
sites
3ey9A

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D

W

A

W

R

E

K

T

G

M

L

E

D

R

L

A

A

M

K

E

P

P

S

A

E

H

K

P

A

I

I

N

H

P

P

Q

Q

R

Y

V

L

F

A

W

Q

E

Q

L

I

S

S

P

H

R

F

L

A

P

A

D

D

N

D

C

A

I

I

L

F

T

T

C

C

D

D

S
x
V

G
|
G

S
x
T

A
x
P

A
x
T

N

V

W

W

Y

A

A

A

R

R

D

Y

L

L

K

K

I

M

-

N

-

G

R

K

Q

R

G

R

M

L

A

G

S

S

L
x
F

S
x
N

G

H

G
|
G

L

-

A

-

T

S

M
|
M

G

A

P

N

G

A

V

M

P

P

Y

Q

A

A

I

L

A

G

A

A

K

Q

F

A

A

T

H

E

P

P

E

E

R

R

V

Q

V

V

I

V

A

A

L

M

I

C

G
|
G

D
|
D

G
|
G

A
x
G

M

F

Q

S

M

M

N

L

G

-

M

M

N

G

E

D

L

F

I

L

T

S

I

V

G

-

K

-

Y

-

W

-

Q

V

R

Q

W

M

R

K

D

L

P

P

R

V

L

K

I

I

V

V

M

-

V

V

L

F

N

N

N
|
N

R

S

D
x
V

L
|
L

N
x
G

Q
x
F

V
|
V

T

A

W

M

E
|
E

Q

-

R

-

A

-

M

M

E

K

G

A

D

G

P

G

K

Y

F

L

N

T

A

D

S

G

Q

T

D

E

V

L

P

H

D

D

F

T

P

N

Y

F

A

A

R

R

M

I

A

A

E

E

L

A

I

C

G

G

L

I

L

T

G

G

I

I

R

R

V

V

D

E

Q

K

P

A

E

S

Q

E

I

V

T

D

D

E

A

A

W

L

E

Q

R

R

A

A

L

F

G

S

A

I

D

D

R

G

P

P

V

V

V

L

L

V

E

D

A

V

Y

V

A

V

D

A

P
x
K

D

E

V

E

P

L

P

A

L

I

P

P

H

Q

I

I

T

K

F

L

K

E

Q

Q

A

A

K

K

A

G

Y

F

A

S

-

L

-
x
Y

-

M

-

L

S

R

A

A

V

I

L

I

K

S

G

G

D

R

P

G

D

D

S

E

V

V

active site: V23 (≠ Y24), G25 (= G26), D26 (= D27), S27 (≠ G28), L28 (≠ I29), E49 (= E51), S72 (≠ T74), F111 (≠ Q113), Q112 (= Q114), G160 (≠ N163), L252 (= L260), A279 (≠ E287), V379 (≠ S389), G405 (= G413), M407 (= M417), D432 (= D442), N459 (= N475), V461 (≠ D477), L462 (= L478), F464 (≠ Q480), V465 (= V481), E468 (= E484), K528 (≠ P547)
binding flavin-adenine dinucleotide: G208 (= G216), S209 (≠ A217), G210 (= G218), A232 (= A240), L233 (= L241), R234 (≠ L242), T250 (≠ I258), G251 (= G259), I253 (≠ L261), G272 (= G280), T273 (≠ S281), Q274 (≠ S282), F275 (= F283), Y277 (= Y285), D291 (= D301), I292 (= I302), S296 (≠ L306), G309 (= G319), D310 (= D320), I311 (≠ S321), T383 (≠ A393), F402 (≠ L410), N403 (≠ S411), Y548 (vs. gap)
binding magnesium ion: D432 (= D442), N459 (= N475)
binding thiamine diphosphate: T24 (≠ P25), E49 (= E51), S72 (≠ T74), G76 (= G78), H79 (= H81), G380 (= G390), T381 (≠ S391), P382 (≠ A392), M407 (= M417), G431 (= G441), D432 (= D442), G433 (= G443), G434 (≠ A444), N459 (= N475), V461 (≠ D477), L462 (= L478), G463 (≠ N479)

2ezuA Pyruvate oxidase variant f479w in complex with reaction intermediate 2-acetyl-thiamin diphosphate (see paper)
30% identity, 92% coverage: 9:550/588 of query aligns to 9:541/585 of 2ezuA

query
sites
2ezuA

L

V

V

I

R

K

R

V

L

L

S

E

E

A

W

W

G

G

V

V

K

D

R

H

I

L

F

Y

G

G

Y
x
I

P

P

G
|
G

D
x
G

G
x
S

I
|
I

N

N

G

S

V

I

F

M

G

D

A

A

L

L

H

S

R

A

A

E

S

R

D

D

N

R

M

I

D

H

F

Y

I

I

Q

Q

V

V

R

R

H

H

E
|
E

E

E

M

V

A

G

A

A

F

M

M

A

A

A

C

A

A

A

H

D

A

A

K

K

F

L

T

T

G

G

E

K

V

I

G

G

V

V

C

C

V

F

A

G

T
x
S

S

A

G

G

P

P

G

G

A

G

I

T

H

H

L

L

L

M

N

N

G

G

L

L

Y

Y

D

D

A

A

K

R

L

E

D

D

H
|
H

Q

V

P

P

V

V

V

L

A

A

I

L

V

I

G

G

Q

Q

Q

F

K

G

R

T

M

T

A

G

L

M

G

N

G

M

N

D

F

T

Q
x
F

Q
|
Q

E
|
E

V

M

D

N

L

E

V

N

T

P

L

I

F

Y

K

A

D

D

V

V

A

A

H

-

E

D

Y

Y

V

N

H

V

M

T

V

A

T

V

T

N

P

A

A

A

Q

T

A

L

R

P

H

H

V

V

I

I

D

D

R

E

A

A

M

I

R

R

I

R

A

A

Q

Y

A

A

E

H

R

Q

N

G

V

V

C

A

C

V

I

V

I

Q

L

I

P

P

N
x
V

D

D

V

L

-

P

-

W

Q

Q

E

Q

M

I

D

P

A

A

E

E

K

D

P

-

R

-

E

W

H

Y

G

A

S

S

I

A

H

N

S

S

G

Y

L

Q

G

T

Y

P

V

L

A

L

P

P

R

E

T

-

V

-

P

P

H

D

A

V

E

Q

E

A

L

V

Q

T

R

R

A

L

A

T

D

Q

I

T

L

L

N

L

A

A

G

A

E

E

K

R

V

P

A

L

M

I

L

Y

V

Y

G
|
G

A
x
I

G
|
G

A

A

L

R

H

K

A

A

S

G

A

K

E

E

I

L

L

E

E

Q

V

L

A

S

D

K

L

T

L

L

G

K

A

I

G

P

I

L

A

M

K

S

A
x
T

L
x
Y

L
x
P

G

A

K

K

A

G

A

I

V

V

P

A

D

D

D

R

L

Y

P

P

Y

A

V

Y

T

L

G

G

S

S

I
x
A

G
x
N

L
x
R

L
x
V

G

A

T

Q

R

K

P

P

S

A

Y

N

D

E

M

A

M

L

T

A

D

Q

C

A

D

D

T

V

L

V

L

L

M

F

V

V

G
|
G

S
x
N

F

Y

P
|
P

Y
x
F

S

A

E
|
E

F

V

L

S

P

K

E

A

E

F

G

K

Q

N

A

T

R

R

-

Y

G

F

V

L

Q

Q

I

I

D
|
D

I
|
I

N

D

G

P

R

A

L
x
K

I

L

G

G

I

K

R

R

Y

H

P

K

M

T

E

D

V

I

N

A

L

V

V

L

G

A

D
|
D

S
x
A

V

Q

E

K

T

T

L

L

R

A

L

A

L

I

M

L

P

A

L

Q

L

V

S

S

K

E

K

R

E

E

G

S

R

T

S

P

W

W

R

W

R

Q

R

A

I

N

E

L

K

A

N

N

V

V

E

K

E

N

W

W

W

R

E

A

T

Y

M

L

E

A

E

S

R

L

A

E

M

D

E

K

P

Q

A

E

H

G

P

P

I

L

N

Q

P

A

Q

Y

R

Q

V

V

F

L

W

R

E

A

L

V

S

N

P

K

R

I

L

A

P

E

D

P

N

D

C

A

I

I

L

Y

T

S

C

I

D

D

S
x
V

G

G

S
x
D

A

I

A

N

N

L

W

N

Y

A

A

N

R

R

D

H

L

L

K

K

I

L

R

T

Q

P

G

S

M

N

M

R

A

H

S

I

L

T

S

S

G
x
N

G

L

L

F

A
|
A

T

T

M
|
M

G

G

P

V

G

G

V

I

P

P

Y

G

A

A

I

I

A

A

K

K

F

L

A

N

H

Y

P

P

E

E

R

R

V

Q

V

V

I

F

A

N

L

L

I

A

G
|
G

D
|
D

G
|
G

A

G

M

A

Q

S

M

M

N

T

G

-

M

M

N

Q

E

D

L

L

I

A

T

T

I

Q

G

V

K

Q

Y

Y

W

-

Q

-

R

-

W

-

R

-

D

H

P

L

R

P

L

V

I

I

V

N

M

V

V

V

L

F

N

T

N
|
N

R

C

D
x
Q

L
x
Y

N
x
G

Q
x
W

V
x
I

T

K

W

D

E
|
E

Q

Q

R

E

A

D

M

T

E

N

G

Q

D

N

P

D

-

F

-

I

-

G

-

V

K

E

F

F

N

N

A

-

S

-

Q

-

D

-

V

-

P

-

D

D

F

I

P

D

Y

F

A

S

R

K

M

I

A

A

E

D

L

G

I

V

G

H

L

M

L

Q

G

A

I

F

R

R

V

V

D

N

Q

K

P

I

E

E

Q

Q

I

L

T

P

D

D

A

V

W

F

E

E

-

Q

-

A

R

K

A

A

L

I

G

A

A

Q

D

H

R

E

P

P

V

V

V

L

L

I

E

D

A

A

Y

V

A

I

D

T

P
x
G

D

D

V

R

P

P

active site: I24 (≠ Y24), G26 (= G26), G27 (≠ D27), S28 (≠ G28), I29 (= I29), E51 (= E51), S74 (≠ T74), F113 (≠ Q113), Q114 (= Q114), E115 (= E115), V162 (≠ N163), R256 (≠ L260), E283 (= E287), V386 (≠ S389), A412 (= A415), M414 (= M417), D439 (= D442), N466 (= N475), Q468 (≠ D477), Y469 (≠ L478), W471 (≠ Q480), I472 (≠ V481), E475 (= E484), G538 (≠ P547)
binding flavin-adenine dinucleotide: H93 (= H93), G212 (= G216), I213 (≠ A217), G214 (= G218), T236 (≠ A240), Y237 (≠ L241), P238 (≠ L242), A254 (≠ I258), N255 (≠ G259), R256 (≠ L260), V257 (≠ L261), G276 (= G280), N277 (≠ S281), N278 (≠ S282), P280 (= P284), F281 (≠ Y285), D298 (= D301), I299 (= I302), K303 (≠ L306), D317 (= D320), A318 (≠ S321), N409 (≠ G412)
binding 2-acetyl-thiamine diphosphate: V386 (≠ S389), D388 (≠ S391), M414 (= M417), G438 (= G441), G440 (= G443), N466 (= N475), Q468 (≠ D477), Y469 (≠ L478), G470 (≠ N479), W471 (≠ Q480), I472 (≠ V481)
binding magnesium ion: D439 (= D442), N466 (= N475), Q468 (≠ D477)

Sites not aligning to the query:

binding pyruvic acid: 547, 549, 550, 554

2ez9A Pyruvate oxidase variant f479w in complex with reaction intermediate analogue 2-phosphonolactyl-thiamin diphosphate (see paper)
30% identity, 92% coverage: 9:550/588 of query aligns to 9:541/585 of 2ez9A

query
sites
2ez9A

L

V

V

I

R

K

R

V

L

L

S

E

E

A

W

W

G

G

V

V

K

D

R

H

I

L

F

Y

G

G

Y
x
I

P

P

G
|
G

D
x
G

G
x
S

I
|
I

N

N

G

S

V

I

F

M

G

D

A

A

L

L

H

S

R

A

A

E

S

R

D

D

N

R

M

I

D

H

F

Y

I

I

Q

Q

V

V

R

R

H

H

E
|
E

E

E

M

V

A

G

A

A

F

M

M

A

A

A

C

A

A

A

H

D

A

A

K

K

F

L

T

T

G

G

E

K

V

I

G

G

V

V

C

C

V

F

A

G

T
x
S

S

A

G

G

P

P

G

G

A

G

I

T

H

H

L

L

L

M

N

N

G

G

L

L

Y

Y

D

D

A

A

K

R

L

E

D

D

H
|
H

Q

V

P

P

V

V

V

L

A

A

I

L

V

I

G

G

Q

Q

Q

F

K

G

R

T

M

T

A

G

L

M

G

N

G

M

N

D

F

T

Q
x
F

Q
|
Q

E
|
E

V

M

D

N

L

E

V

N

T

P

L

I

F

Y

K

A

D

D

V

V

A

A

H

-

E

D

Y

Y

V

N

H

V

M

T

V

A

T

V

T

N

P

A

A

A

Q

T

A

L

R

P

H

H

V

V

I

I

D

D

R

E

A

A

M

I

R

R

I

R

A

A

Q

Y

A

A

E

H

R

Q

N

G

V

V

C

A

C

V

I

V

I

Q

L

I

P

P

N
x
V

D

D

V

L

-

P

-

W

Q

Q

E

Q

M

I

D

P

A

A

E

E

K

D

P

-

R

-

E

W

H

Y

G

A

S

S

I

A

H

N

S

S

G

Y

L

Q

G

T

Y

P

V

L

A

L

P

P

R

E

T

-

V

-

P

P

H

D

A

V

E

Q

E

A

L

V

Q

T

R

R

A

L

A

T

D

Q

I

T

L

L

N

L

A

A

G

A

E

E

K

R

V

P

A

L

M

I

L

Y

V

Y

G
|
G

A
x
I

G
|
G

A

A

L

R

H

K

A

A

S

G

A

K

E

E

I

L

L

E

E

Q

V

L

A

S

D

K

L

T

L

L

G

K

A

I

G

P

I

L

A

M

K

S

A
x
T

L
x
Y

L
x
P

G

A

K

K

A

G

A

I

V

V

P

A

D

D

D

R

L

Y

P

P

Y

A

V

Y

T

L

G

G

S

S

I
x
A

G
x
N

L
x
R

L
x
V

G

A

T

Q

R

K

P

P

S

A

Y

N

D

E

M

A

M

L

T

A

D

Q

C

A

D

D

T

V

L

V

L

L

M

F

V

V

G
|
G

S
x
N

F

Y

P
|
P

Y
x
F

S

A

E
|
E

F

V

L

S

P

K

E

A

E

F

G

K

Q

N

A

T

R

R

-

Y

G

F

V

L

Q

Q

I

I

D
|
D

I
|
I

N

D

G

P

R

A

L
x
K

I

L

G

G

I

K

R

R

Y

H

P

K

M

T

E

D

V

I

N

A

L

V

V

L

G

A

D
|
D

S
x
A

V

Q

E

K

T

T

L

L

R

A

L

A

L

I

M

L

P

A

L

Q

L

V

S

S

K

E

K

R

E

E

G

S

R

T

S

P

W

W

R

W

R

Q

R

A

I

N

E

L

K

A

N

N

V

V

E

K

E

N

W

W

W

R

E

A

T

Y

M

L

E

A

E

S

R

L

A

E

M

D

E

K

P

Q

A

E

H

G

P

P

I

L

N

Q

P

A

Q

Y

R

Q

V

V

F

L

W

R

E

A

L

V

S

N

P

K

R

I

L

A

P

E

D

P

N

D

C

A

I

I

L

Y

T

S

C

I

D

D

S
x
V

G

G

S
x
D

A

I

A

N

N

L

W

N

Y

A

A

N

R

R

D

H

L

L

K

K

I

L

R

T

Q

P

G

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M

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H

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I

L

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S

S

G
x
N

G

L

L

F

A
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A

T

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M
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M

G

G

P

V

G

G

V

I

P

P

Y

G

A

A

I

I

A

A

K

K

F

L

A

N

H

Y

P

P

E

E

R

R

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Q

V

V

I

F

A

N

L

L

I

A

G
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G

D
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D

G
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G

A

G

M

A

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S

M

M

N

T

G

-

M

M

N

Q

E

D

L

L

I

A

T

T

I

Q

G

V

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Q

Y

Y

W

-

Q

-

R

-

W

-

R

-

D

H

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L

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L

V

I

I

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N

M

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E

A

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E

N

G

Q

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N

P

D

-

F

-

I

-

G

-

V

K

E

F

F

N

N

A

-

S

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-

D

D

F

I

P

D

Y

F

A

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I

A

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E

D

L

G

I

V

G

H

L

M

L

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G

A

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R

R

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V

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N

Q

K

P

I

E

E

Q

Q

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L

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D

D

A

V

W

F

E

E

-

Q

-

A

R

K

A

A

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A

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P

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L

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x
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D

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R

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P

active site: I24 (≠ Y24), G26 (= G26), G27 (≠ D27), S28 (≠ G28), I29 (= I29), E51 (= E51), S74 (≠ T74), F113 (≠ Q113), Q114 (= Q114), E115 (= E115), V162 (≠ N163), R256 (≠ L260), E283 (= E287), V386 (≠ S389), A412 (= A415), M414 (= M417), D439 (= D442), N466 (= N475), Q468 (≠ D477), Y469 (≠ L478), W471 (≠ Q480), I472 (≠ V481), E475 (= E484), G538 (≠ P547)
binding flavin-adenine dinucleotide: H93 (= H93), G212 (= G216), I213 (≠ A217), G214 (= G218), T236 (≠ A240), Y237 (≠ L241), P238 (≠ L242), A254 (≠ I258), N255 (≠ G259), R256 (≠ L260), V257 (≠ L261), G276 (= G280), N277 (≠ S281), N278 (≠ S282), P280 (= P284), F281 (≠ Y285), D298 (= D301), I299 (= I302), K303 (≠ L306), D317 (= D320), A318 (≠ S321), N409 (≠ G412)
binding magnesium ion: D439 (= D442), N466 (= N475), Q468 (≠ D477)
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-{(1s)-1-hydroxy-1-[(r)-hydroxy(methoxy)phosphoryl]ethyl}-5-(2-{[(s)-hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: V386 (≠ S389), D388 (≠ S391), M414 (= M417), G438 (= G441), G440 (= G443), N466 (= N475), Q468 (≠ D477), Y469 (≠ L478), G470 (≠ N479), W471 (≠ Q480), I472 (≠ V481), E475 (= E484)

2ez8A Pyruvate oxidase variant f479w in complex with reaction intermediate 2-lactyl-thiamin diphosphate (see paper)
30% identity, 92% coverage: 9:550/588 of query aligns to 9:541/585 of 2ez8A

query
sites
2ez8A

L

V

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V

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L

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E

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Y
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I

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I

N

N

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V

I

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D

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E
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E

E

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M

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A

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C

A

A

A

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D

A

A

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K

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L

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G

G

E

K

V

I

G

G

V

V

C

C

V

F

A

G

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x
S

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A

G

G

P

P

G

G

A

G

I

T

H

H

L

L

L

M

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N

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Y

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A

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A

A

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L

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G

N

G

M

N

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E
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N

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A

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G

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-

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x
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W

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E

Q

Q

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E

A

D

M

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E

N

G

Q

D

N

P

D

-

F

-

I

-

G

-

V

K

E

F

F

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N

A

-

S

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-

D

-

V

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-

D

D

F

I

P

D

Y

F

A

S

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M

I

A

A

E

D

L

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I

V

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L

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G

A

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F

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R

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N

Q

K

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I

E

E

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Q

I

L

T

P

D

D

A

V

W

F

E

E

-

Q

-

A

R

K

A

A

L

I

G

A

A

Q

D

H

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E

P

P

V

V

V

L

L

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P

active site: I24 (≠ Y24), G26 (= G26), G27 (≠ D27), S28 (≠ G28), I29 (= I29), E51 (= E51), S74 (≠ T74), F113 (≠ Q113), Q114 (= Q114), E115 (= E115), V162 (≠ N163), R256 (≠ L260), E283 (= E287), V386 (≠ S389), A412 (= A415), M414 (= M417), D439 (= D442), N466 (= N475), Q468 (≠ D477), Y469 (≠ L478), W471 (≠ Q480), I472 (≠ V481), E475 (= E484), G538 (≠ P547)
binding flavin-adenine dinucleotide: H93 (= H93), G212 (= G216), I213 (≠ A217), G214 (= G218), T236 (≠ A240), Y237 (≠ L241), P238 (≠ L242), A254 (≠ I258), N255 (≠ G259), R256 (≠ L260), V257 (≠ L261), G276 (= G280), N277 (≠ S281), N278 (≠ S282), P280 (= P284), F281 (≠ Y285), D298 (= D301), I299 (= I302), K303 (≠ L306), D317 (= D320), A318 (≠ S321), N390 (≠ A393), N409 (≠ G412)
binding magnesium ion: D439 (= D442), N466 (= N475), Q468 (≠ D477)
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-carboxy-1-hydroxyethyl)-5-(2-{[hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: D388 (≠ S391), M414 (= M417), G438 (= G441), G440 (= G443), N466 (= N475), Q468 (≠ D477), Y469 (≠ L478), G470 (≠ N479), W471 (≠ Q480), I472 (≠ V481)

Sites not aligning to the query:

binding pyruvic acid: 547, 549, 550, 553, 554

2ez4B Pyruvate oxidase variant f479w (see paper)
30% identity, 92% coverage: 9:550/588 of query aligns to 9:541/585 of 2ez4B

query
sites
2ez4B

L

V

V

I

R

K

R

V

L

L

S

E

E

A

W

W

G

G

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I

L

F

Y

G

G

Y
x
I

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P

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G

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x
G

G
x
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I

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N

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A

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M

A

A

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C

A

A

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A

A

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C

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x
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A

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P

G

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A

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H

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M

N

N

G

G

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L

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Y

D

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active site: I24 (≠ Y24), G26 (= G26), G27 (≠ D27), S28 (≠ G28), I29 (= I29), E51 (= E51), S74 (≠ T74), F113 (≠ Q113), Q114 (= Q114), E115 (= E115), V162 (≠ N163), R256 (≠ L260), E283 (= E287), V386 (≠ S389), A412 (= A415), M414 (= M417), D439 (= D442), N466 (= N475), Q468 (≠ D477), Y469 (≠ L478), W471 (≠ Q480), I472 (≠ V481), E475 (= E484), G538 (≠ P547)
binding flavin-adenine dinucleotide: H93 (= H93), G212 (= G216), I213 (≠ A217), G214 (= G218), T236 (≠ A240), Y237 (≠ L241), P238 (≠ L242), A254 (≠ I258), N255 (≠ G259), R256 (≠ L260), V257 (≠ L261), G276 (= G280), N277 (≠ S281), N278 (≠ S282), P280 (= P284), F281 (≠ Y285), D298 (= D301), I299 (= I302), K303 (≠ L306), D317 (= D320), A318 (≠ S321), N409 (≠ G412)
binding magnesium ion: D439 (= D442), N466 (= N475), Q468 (≠ D477)
binding phosphate ion: W471 (≠ Q480), E475 (= E484)
binding thiamine diphosphate: D388 (≠ S391), A412 (= A415), M414 (= M417), G438 (= G441), D439 (= D442), G440 (= G443), G441 (≠ A444), N466 (= N475), Q468 (≠ D477),