SitesBLAST

P78330 Phosphoserine phosphatase; PSP; PSPase; L-3-phosphoserine phosphatase; O-phosphoserine phosphohydrolase; EC 3.1.3.3 from Homo sapiens (Human) (see 4 papers)
39% identity, 93% coverage: 2:199/213 of query aligns to 13:212/225 of P78330

query
sites
P78330

S

S

F

A

D

D

V

A

I

V

C

C

F

F

D
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D

C
x
V

D
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D

S
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S

T

T

L

V

S

I

K

R

I

E

E
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E

G

G

I

I

D
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D

E

E

L

L

A
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A

G

K

R

I

V

C

G

G

L

V

G

E

E

D

E

A

M

V

S

S

R

E

L

M

T

T

D

R

A

R

A

A

M
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M

N
x
G

G

G

L

A

V

V

P

P

L

F

E

K

A

A

V

A

Y

L

E

T

K

E

R
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R

L

L

S

A

L

L

I

I

R

Q

P

P

D

S

R

R

A

E

G

Q

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D

Q

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L

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I

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Y

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G

A

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E

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L

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Q

A

E

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R

N

N

K

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E

Q

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H

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I

L

I

I

S
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S

G
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G

I

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R

Q

S

A

I

I

V

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E

P

H

L

V

A

A

G

S

F

K

L

L

G

N

L

I

P

P

E

A

S

T

R

N

V

V

H

F

A

A

V
x
N

D

R

V

L

Y

K

F

F

N

Y

E

F

D

N

G

G

S

E

Y

Y

R

A

G

G

Y

F

D

D

Q

E

S

T

S

Q

P

P

L

T

A

A

R

E

T

S

G

G

K
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K

A

G

E

K

I

V

C

I

R

-

R

K

L

L

V

L

K

K

P

E

E

K

V

F

-

H

-

F

-

K

P

K

L

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M

M

I

I

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D
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D

G

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A

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T

D

D

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M

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E

A

A

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C

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P

E

P

G

A

V

D

S

A

V

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I

I

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G

F

F

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N

V

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R

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A

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Y

Y

D20 (= D9) binding Mg(2+)
DVD 20:22 (≠ DCD 9:11) binding L-serine
D22 (= D11) binding Mg(2+)
S23 (= S12) mutation to A: Reduces L-phosphoserine phosphatase activity by about 50%.; mutation to T: Reduces L-phosphoserine phosphatase activity by about 80%.
E29 (= E18) mutation to D: Reduces L-phosphoserine phosphatase activity by about 95%.; mutation to Q: Loss of L-phosphoserine phosphatase activity.
D32 (= D21) to N: in PSPHD; decreased L-phosphoserine phosphatase activity; dbSNP:rs104894035
A35 (= A24) to T: in PSPHD; decreased L-phosphoserine phosphatase activity
M52 (= M41) binding O-phospho-L-serine; to T: in PSPHD; decreased L-phosphoserine phosphatase activity; dbSNP:rs104894036
G53 (≠ N42) binding phosphate
R65 (= R54) mutation R->A,K: Loss of L-phosphoserine phosphatase activity.
SGG 109:111 (= SGG 98:100) binding L-serine; binding O-phospho-L-serine
N133 (≠ V122) mutation to A: Reduces L-phosphoserine phosphatase activity by about 75%.
K158 (= K147) binding L-serine; binding O-phospho-L-serine
D179 (= D166) binding Mg(2+)
T182 (= T169) binding O-phospho-L-serine; binding phosphate; mutation to S: Reduces L-phosphoserine phosphatase activity by about 99%.; mutation to V: Reduces L-phosphoserine phosphatase activity by about 25%.
R202 (= R189) mutation to A: Reduces L-phosphoserine phosphatase activity by about 99%.; mutation to K: Reduces L-phosphoserine phosphatase activity by about 95%.

6hyyA Human phosphoserine phosphatase with serine and phosphate (see paper)
39% identity, 93% coverage: 2:199/213 of query aligns to 9:208/221 of 6hyyA

query
sites
6hyyA

S

S

F

A

D

D

V

A

I

V

C

C

F

F

D
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D

C
x
V

D
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D

S

S

T

T

L

V

S

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K

R

I

E

E

E

G

G

I

I

D

D

E

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L

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A

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R

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G

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H

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A

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D

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K

F

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Y

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A

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G

Y

F

D

D

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P

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K

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-

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P

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M

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D

G

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D

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A

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C

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F

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V

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R

K

E

D

L

N

A

A

D

K

F

W

Y

Y

binding magnesium ion: D16 (= D9), D18 (= D11), D175 (= D166)
binding serine: D16 (= D9), V17 (≠ C10), D18 (= D11), S105 (= S98), G106 (= G99), K154 (= K147)

1l8oA Molecular basis for the local conformational rearrangement of human phosphoserine phosphatase (see paper)
39% identity, 93% coverage: 2:199/213 of query aligns to 10:209/222 of 1l8oA

query
sites
1l8oA

S

S

F

A

D

D

V

A

I

V

C

C

F

F

D
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D

C
x
V

D
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D

S

S

T

T

L

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K

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E

E

G

G

I

I

D

D

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L

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G

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A

R

A

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M

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L

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R

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N

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S

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G

G

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L

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A

A

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H

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D

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F

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D

N

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Y

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F

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D

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K

A

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M

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A

A

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Y

Y

active site: D17 (= D9), V18 (≠ C10), D19 (= D11), G107 (= G99), K155 (= K147), D180 (= D170)
binding phosphate ion: D17 (= D9), D19 (= D11), S106 (= S98), K155 (= K147)
binding serine: G177 (= G167), T179 (= T169), R199 (= R189)

1l8lA Molecular basis for the local confomational rearrangement of human phosphoserine phosphatase (see paper)
39% identity, 93% coverage: 2:199/213 of query aligns to 10:209/222 of 1l8lA

query
sites
1l8lA

S

S

F

A

D

D

V

A

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V

C

C

F

F

D
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D

C
x
V

D
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D

S

S

T

T

L

V

S

I

K

R

I

E

E

E

G

G

I

I

D

D

E

E

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L

A

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A

R

A

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H

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A

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A

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K

F

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G

G

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Y

Y

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G

Y

F

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D

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L

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G

K
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K

A

G

E

K

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C

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K

K

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K

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M

M

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I

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D
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D

G
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K

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D

M

M

E

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A

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K

C

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E

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A

V

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F

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N

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R

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E

D

L

N

A

A

D

K

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W

Y

Y

active site: D17 (= D9), V18 (≠ C10), D19 (= D11), G107 (= G99), K155 (= K147), D180 (= D170)
binding d-2-amino-3-phosphono-propionic acid: D17 (= D9), D19 (= D11), G107 (= G99), K155 (= K147), D176 (= D166), G177 (= G167), T179 (= T169)

6iuyA Structure of dsgpdh of dunaliella salina (see paper)
31% identity, 92% coverage: 4:199/213 of query aligns to 15:212/585 of 6iuyA

query
sites
6iuyA

D

D

V

A

I

V

C

C

F

F

D
|
D

C

V

D
|
D

S

R

T

T

L

V

S

T

K

T

I

D

E

A

G

S

I

V

D

G

E

L

L

L

A

A

G

K

R

F

V

M

G

G

L

I

G

E

E

D

E

E

M

A

S

Q

R

S

L

L

T

T

D

E

A

Q

A

A

M

N

N

R

G

G

L

E

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I

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N

L

L

E

T

A

K

V

A

Y

F

E

E

K

D

R

R

L

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S

A

L

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I

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R

N

P

F

D

T

R

P

A

T

G

D

I

I

D

D

W

R

L

F

A

L

R

E

L

E

Y

H

-

P

-

A

I

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S

T

E

R

I

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V

V

E

P

G

G

A

V

A

E

E

N

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A

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L

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K

A

A

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G

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E

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I

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P

L

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A

A

G

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H

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A

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N

V

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H

-

A

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D

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Y

-

F

F

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C

E

N

D

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M

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Y

W

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Y

D

D

D

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G

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K

A

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I

A

C

I

R

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R

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K

R

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K

-

Y

P

P

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Y

V

N

P

N

L

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M

M

I

V

G

G

D
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D

G

G

K

F

T

S

D

D

M

L

E

E

A

A

K

M

Q

Q

-

G

-

S

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P

E

D

G

G

V

A

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D

S

A

V

F

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I

G

C

F

F

G

G

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V

V

M

A

Q

R

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P

P

I

A

V

V

R

A

E

S

L

Q

A

A

D

D

F

W

Y

F

binding magnesium ion: D20 (= D9), D22 (= D11), D175 (= D166)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 233, 234, 235, 236, 268, 290, 321, 324, 349, 381, 382, 497, 529, 530, 532

1l7nA Transition state analogue of phosphoserine phosphatase (aluminum fluoride complex) (see paper)
31% identity, 98% coverage: 5:212/213 of query aligns to 5:208/209 of 1l7nA

query
sites
1l7nA

V

L

I

I

C

L

F

F

D
|
D

C
x
F

D
|
D

S

S

T

T

L

L

S

V

K

N

I

N

E

E

G

T

I

I

D

D

E

E

L

I

A

A

G

R

R

E

V

A

G

G

L

V

G

E

E

E

M

V

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K

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K

L

I

T

T

D

K

A

E

A

A

M

M

N

E

G

G

L

K

V

L

P

N

L

F

E

E

A

Q

V

S

Y

L

E

R

K

K

R

R

L

V

S

S

L

L

I

L

R

K

-

D

-

L

P

P

D

I

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E

A

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I

E

D

K

W

A

L

I

A

K

R

R

L

I

Y

T

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P

S

T

E

-

I

-

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-

E

E

G

G

A

A

A

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E

E

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F

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S

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L

L

S

K

A

N

R

R

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G

K

Y

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V

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H

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S
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S

G
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G

G

G

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D

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A

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L

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K

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L

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N

A

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Y

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F

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N

-

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K

D

D

G

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S

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Y

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G

G

Y

-

D

D

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L

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E

G

N

G

A

K
|
K

A

G

E

E

I

I

C

L

R

E

R

K

L

I

V

A

K

K

P

I

E

E

-

G

-

I

-

N

-

L

V

E

P

D

L

T

L

V

M

A

I

V

G

G

D
|
D

G

G

K

A

T
x
N

D
|
D

M

I

E

S

A

M

K

F

Q

K

E

K

G

A

V

G

S

L

V

K

I

I

G

A

F

F

G

-

V

-

V

C

A

A

R

K

P

P

I

I

V

L

R

K

E

E

L

K

A

A

D

D

F

I

Y

C

F

I

D

E

H

K

P

R

T

D

L

L

L

R

P

E

I

I

L

L

S

K

Y

Y

I

I

active site: D9 (= D9), F10 (≠ C10), D11 (= D11), G98 (= G99), K142 (= K147), D169 (= D170)
binding aluminum fluoride: D9 (= D9), F10 (≠ C10), D11 (= D11), S97 (= S98), K142 (= K147)
binding tetrafluoroaluminate ion: D9 (= D9), F10 (≠ C10), D11 (= D11), S97 (= S98), G98 (= G99), K142 (= K147), N168 (≠ T169)
binding magnesium ion: D9 (= D9), D11 (= D11), D165 (= D166)

Q58989 Phosphoserine phosphatase; PSP; PSPase; O-phosphoserine phosphohydrolase; EC 3.1.3.3 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see 3 papers)
31% identity, 98% coverage: 5:212/213 of query aligns to 7:210/211 of Q58989

query
sites
Q58989

V

L

I

I

C

L

F

F

D
|
D

C

F

D
|
D

S

S

T

T

L

L

S

V

K

N

I

N

E
|
E

G

T

I

I

D

D

E

E

L

I

A

A

G

R

R

E

V

A

G

G

L

V

G

E

E

E

M

V

S

K

R

K

L

I

T

T

D

K

A

E

A

A

M

M

N

E

G

G

L

K

V

L

P

N

L

F

E

E

A

Q

V

S

Y

L

E

R

K

K

R
|
R

L

V

S

S

L

L

I

L

R

K

-

D

-

L

P

P

D

I

R

E

A

K

G

V

I

E

D

K

W

A

L

I

A

K

R

R

L

I

Y

T

I

P

S

T

E

-

I

-

V

-

E

E

G

G

A

A

A

E

E

E

V

T

F

I

S

K

S

E

L

L

S

K

A

N

R

R

N

G

K

Y

E

V

L

V

H

A

I

V

S
|
S

G
|
G

G

G

I

F

R

D

Q

I

A

A

I

V

L

N

P

K

L

I

A

K

G

E

F

K

L

L

G

G

L

L

P

D

E

Y

S

A

R

F

V

A

H

N

A

R

V

L

D

I

V

V

Y

-

F

-

N

-

E

K

D

D

G

G

S

K

Y

L

R

T

G

G

Y

-

D

D

Q

V

S

E

S

G

P

E

L

V

A

L

R

K

T

E

G

N

G

A

K
|
K

A

G

E

E

I

I

C

L

R

E

R

K

L

I

V

A

K

K

P

I

E

E

-

G

-

I

-

N

-

L

V

E

P

D

L

T

L

V

M

A

I

V

G

G

D
|
D

G

G

K

A

T
x
N

D

D

M

I

E

S

A

M

K

F

Q

K

E

K

G

A

V

G

S

L

V

K

I

I

G

A

F

F

G

-

V

-

V

C

A

A

R

K

P

P

I

I

V

L

R

K

E

E

L

K

A

A

D

D

F

I

Y

C

F

I

D

E

H

K

P

R

T

D

L

L

L

R

P

E

I

I

L

L

S

K

Y

Y

I

I

D11 (= D9) active site, Nucleophile; binding Mg(2+); mutation to N: Loss of activity.
D13 (= D11) active site, Proton donor; binding Mg(2+)
E20 (= E18) binding substrate
R56 (= R54) binding substrate
SG 99:100 (= SG 98:99) binding substrate
K144 (= K147) binding substrate
D167 (= D166) binding Mg(2+)
N170 (≠ T169) binding substrate

1f5sA Crystal structure of phosphoserine phosphatase from methanococcus jannaschii (see paper)
31% identity, 98% coverage: 5:212/213 of query aligns to 6:209/210 of 1f5sA

query
sites
1f5sA

V

L

I

I

C

L

F

F

D
|
D

C
x
F

D
|
D

S

S

T

T

L

L

S

V

K

N

I

N

E

E

G

T

I

I

D

D

E

E

L

I

A

A

G

R

R

E

V

A

G

G

L

V

G

E

E

E

M

V

S

K

R

K

L

I

T

T

D

K

A

E

A

A

M

M

N

E

G

G

L

K

V

L

P

N

L

F

E

E

A

Q

V

S

Y

L

E

R

K

K

R

R

L

V

S

S

L

L

I

L

R

K

-

D

-

L

P

P

D

I

R

E

A

K

G

V

I

E

D

K

W

A

L

I

A

K

R

R

L

I

Y

T

I

P

S

T

E

-

I

-

V

-

E

E

G

G

A

A

A

E

E

E

V

T

F

I

S

K

S

E

L

L

S

K

A

N

R

R

N

G

K

Y

E

V

L

V

H

A

I

V

S
|
S

G
|
G

G

G

I

F

R

D

Q

I

A

A

I

V

L

N

P

K

L

I

A

K

G

E

F

K

L

L

G

G

L

L

P

D

E

Y

S

A

R

F

V

A

H

N

A

R

V

L

D

I

V

V

Y

-

F

-

N

-

E

K

D

D

G

G

S

K

Y

L

R

T

G

G

Y

-

D

D

Q

V

S

E

S

G

P

E

L

V

A

L

R

K

T

E

G

N

G

A

K
|
K

A

G

E

E

I

I

C

L

R

E

R

K

L

I

V

A

K

K

P

I

E

E

-

G

-

I

-

N

-

L

V

E

P

D

L

T

L

V

M

A

I

V

G

G

D
|
D

G

G

K

A

T

N

D
|
D

M

I

E

S

A

M

K

F

Q

K

E

K

G

A

V

G

S

L

V

K

I

I

G

A

F

F

G

-

V

-

V

C

A

A

R

K

P

P

I

I

V

L

R

K

E

E

L

K

A

A

D

D

F

I

Y

C

F

I

D

E

H

K

P

R

T

D

L

L

L

R

P

E

I

I

L

L

S

K

Y

Y

I

I

active site: D10 (= D9), F11 (≠ C10), D12 (= D11), G99 (= G99), K143 (= K147), D170 (= D170)
binding magnesium ion: D10 (= D9), D12 (= D11), D166 (= D166)
binding phosphate ion: D10 (= D9), F11 (≠ C10), D12 (= D11), S98 (= S98), G99 (= G99), K143 (= K147)

3kd3A Crystal structure of a phosphoserine phosphohydrolase-like protein from francisella tularensis subsp. Tularensis schu s4
28% identity, 91% coverage: 6:198/213 of query aligns to 4:203/216 of 3kd3A

query
sites
3kd3A

I

I

C

I

F

F

D
|
D

C
x
F

D
|
D

S

S

T

T

L

L

S

I

K

K

I

K

E

E

G

S

I

L

D

E

E

L

-

I

-

L

-

E

-

P

-

I

L

L

A

Q

G

K

R

S

V

P

G

A

L

K

G

L

E

K

E

E

M

I

S

E

R

Y

L

I

T

T

D

N

A

L

A

G

M

M

N

Q

G

G

L

D

V

I

P

S

L

F

E

R

A

D

V

S

Y

L

E

Q

K

K

R

R

L

L

S

A

L

I

I

A

R

S

P

P

D

T

R

K

A

Q

G

S

I

I

D

K

W

E

L

F

A

S

R

N

L

K

Y

Y

I

C

S

P

E

N

I

L

V

L

-

T

E

D

G

G

A

I

A

K

E

E

V

L

F

V

S

Q

S

D

L

L

S

K

A

N

R

K

N

G

K

F

E

E

L

I

H

W

I

I

I

F

S

S

G
|
G

G

G

I

L

R

S

Q

E

A

S

I

I

L

Q

P

P

L

F

A

A

G

D

F

Y

L

L

G

N

L

I

P

P

E

R

S

E

R

N

V

I

H

F

A

A

V

V

D

E

V

T

Y

I

F

W

N

N

E

S

D

D

G

G

S

S

Y

F

R

K

G

E

Y

L

D

D

Q

N

S

S

S

N

P

G

L

A

A

C

R

D

T

S

G

K

G

L

K

S

A

A

-

F

E

D

I

K

C

A

R

K

R

G

L

L

V

I

K

D

P

G

E

E

V

V

P

-

L

-

L

I

M

A

I

I

G

G

D
|
D

G

G

K

Y

T

T

D
|
D

M

Y

E

Q

A

L

K

Y

Q

E

E

K

G

G

V

Y

S

A

V

T

-

K

-

F

I

I

G

A

F

Y

G

M

G

E

V

H

V

I

A

E

R

R

P

E

I

K

V

V

R

I

E

N

L

L

A

S

D

K

F

Y

active site: D7 (= D9), F8 (≠ C10), D9 (= D11), G103 (= G99), D173 (= D170)
binding magnesium ion: D7 (= D9), D9 (= D11), D169 (= D166)

1l7pA Substrate bound phosphoserine phosphatase complex structure (see paper)
30% identity, 98% coverage: 5:212/213 of query aligns to 4:207/208 of 1l7pA

query
sites
1l7pA

V

L

I

I

C

L

F

F

D
x
N

C
x
F

D
|
D

S

S

T

T

L

L

S

V

K

N

I

N

E
|
E

G

T

I

I

D

D

E

E

L

I

A

A

G

R

R

E

V

A

G

G

L

V

G

E

E

E

M

V

S

K

R

K

L

I

T

T

D

K

A

E

A

A

M
|
M

N

E

G

G

L

K

V

L

P

N

L
x
F

E

E

A

Q

V

S

Y

L

E

R

K

K

R
|
R

L

V

S

S

L

L

I

L

R

K

-

D

-

L

P

P

D

I

R

E

A

K

G

V

I

E

D

K

W

A

L

I

A

K

R

R

L

I

Y

T

I

P

S

T

E

-

I

-

V

-

E

E

G

G

A

A

A

E

E

E

V

T

F

I

S

K

S

E

L

L

S

K

A

N

R

R

N

G

K

Y

E

V

L

V

H

A

I

V

S
|
S

G
|
G

G

G

I

F

R

D

Q

I

A

A

I

V

L

N

P

K

L

I

A

K

G

E

F

K

L

L

G

G

L

L

P

D

E

Y

S

A

R

F

V

A

H

N

A

R

V

L

D

I

V

V

Y

-

F

-

N

-

E

K

D

D

G

G

S

K

Y

L

R

T

G

G

Y

-

D

D

Q

V

S

E

S

G

P

E

L

V

A

L

R

K

T

E

G

N

G

A

K
|
K

A

G

E

E

I

I

C

L

R

E

R

K

L

I

V

A

K

K

P

I

E

E

-

G

-

I

-

N

-

L

V

E

P

D

L

T

L

V

M

A

I

V

G

G

D

D

G

G

K

A

T

N

D
|
D

M

I

E

S

A

M

K

F

Q

K

E

K

G

A

V

G

S

L

V

K

I

I

G

A

F

F

G

-

V

-

V

C

A

A

R

K

P

P

I

I

V

L

R

K

E

E

L

K

A

A

D

D

F

I

Y

C

F

I

D

E

H

K

P

R

T

D

L

L

L

R

P

E

I

I

L

L

S

K

Y

Y

I

I

active site: N8 (≠ D9), F9 (≠ C10), D10 (= D11), G97 (= G99), K141 (= K147), D168 (= D170)
binding phosphoserine: N8 (≠ D9), F9 (≠ C10), D10 (= D11), E17 (= E18), M40 (= M41), F46 (≠ L47), R53 (= R54), S96 (= S98), G97 (= G99), K141 (= K147)

1l7oA Crystal structure of phosphoserine phosphatase in apo form (see paper)
29% identity, 98% coverage: 5:212/213 of query aligns to 4:199/200 of 1l7oA

query
sites
1l7oA

V

L

I

I

C

L

F

F

D

N

C

F

D

D

S

S

T

T

L

L

S

V

K

N

I

N

E

E

G

T

I

I

D

D

E

E

L

I

A

A

G

R

R

E

V

A

G

G

L

V

G

E

E

E

M

V

S

K

R

K

L

I

T

T

D

N

A

-

M

-

N

-

G

-

L

-

V

-

P

-

L

F

E

E

A

Q

V

S

Y

L

E

R

K

K

R

R

L

V

S

S

L

L

I

L

R

K

-

D

-

L

P

P

D

I

R

E

A

K

G

V

I

E

D

K

W

A

L

I

A

K

R

R

L

I

Y

T

I

P

S

T

E

-

I

-

V

-

E

E

G

G

A

A

A

E

E

E

V

T

F

I

S

K

S

E

L

L

S

K

A

N

R

R

N

G

K

Y

E

V

L

V

H

A

I

V

S

S

G

G

I

F

R

D

Q

I

A

A

I

V

L

N

P

K

L

I

A

K

G

E

F

K

L

L

G

G

L

L

P

D

E

Y

S

A

R

F

V

A

H

N

A

R

V

L

D

I

V

V

Y

-

F

-

N

-

E

K

D

D

G

G

S

K

Y

L

R

T

G

G

Y

-

D

D

Q

V

S

E

S

G

P

E

L

V

A

L

R

K

T

E

G

N

G

A

K

K

A

G

E

E

I

I

C

L

R

E

R

K

L

I

V

A

K

K

P

I

E

E

-

G

-

I

-

N

-

L

V

E

P

D

L

T

L

V

M

A

I

V

G

G

D

D

G

G

K

A

T

N

D

D

M

I

E

S

A

M

K

F

Q

K

E

K

G

A

V

G

S

L

V

K

I

I

G

A

F

F

G

-

V

-

V

C

A

A

R

K

P

P

I

I

V

L

R

K

E

E

L

K

A

A

D

D

F

I

Y
x
C

F

I

D
x
E

H

K

P

R

T

D

L

L

L

R

P

E

I

I

L

L

S

K

Y

Y

I

I

binding zinc ion: C186 (≠ Y199), E188 (≠ D201)

5jlpA Crystal structure of mycobacterium avium serb2 in complex with serine at act domain
30% identity, 96% coverage: 5:209/213 of query aligns to 179:379/396 of 5jlpA

query
sites
5jlpA

V

L

I

I

C

V

F

F

D
|
D

C
x
V

D
|
D

S

S

T

T

L

L

S

V

K

Q

I

G

E

E

G

V

I

I

D

E

E

M

L

L

A

A

G

A

R

K

V

A

G

G

L

A

G

E

E

G

E

Q

M

V

S

A

R

A

L

I

T

T

D

D

A

A

M

M

N

R

G

G

L

E

V

L

P

D

L

F

E

A

A

Q

V

S

Y

L

E

Q

K

Q

R

R

L

V

S

A

L

T

I

L

R

A

P

G

D

L

R

P

A

A

G

T

-

V

I

I

D

D

W

E

L

V

A

A

R

G

L

Q

Y

L

I

-

S

-

E

E

I

L

V

M

E

P

G

G

A

A

A

R

E

T

V

T

F

L

S

R

S

T

L

L

S

R

A

R

R

L

N

G

K

Y

E

A

L

C

H

G

I

V

S

S

G
|
G

G

G

I

F

R

R

Q

R

A

I

I

I

L

E

P

P

L

L

A

A

G

E

F

E

L

L

G

M

L

L

P

D

E

Y

S

V

R

A

V

A

H

N

A

E

V

L

D

E

V

I

Y

V

F

-

N

-

E

-

D

D

G

G

S

T

Y

L

R

T

G

G

Y

R

D

-

Q

V

S

V

S

G

P

P

L

I

A

I

R

D

T

R

G

A

G

G

K
|
K

A

A

-

T

-

A

-

L

-

R

E

E

I

F

C

A

R

Q

R

R

L

A

V

G

K

V

P

P

E

M

V

A

P

Q

L

T

L

V

M

A

I

V

G

G

D
|
D

G

G

K

A

T

N

D
|
D

M

I

E

D

A

M

K

L

Q

A

E

A

G

A

V

G

S

L

V

G

I

I

G

A

F

F

G

N

G

-

V

-

A

A

R

K

P

P

I

A

V

L

R

R

E

E

L

V

A

A

D

D

F

A

Y

S

F

L

D

S

H

H

P

P

T

Y

L

L

L

D

P

T

I

V

L

L

active site: D183 (= D9), V184 (≠ C10), D185 (= D11), G272 (= G99), K316 (= K147), D343 (= D170)
binding magnesium ion: D183 (= D9), D339 (= D166)

Sites not aligning to the query:

binding serine: 13, 14

A0QJI1 Phosphoserine phosphatase; PSP; PSPase; O-phosphoserine phosphohydrolase; EC 3.1.3.3 from Mycobacterium avium (strain 104) (see paper)
30% identity, 96% coverage: 5:209/213 of query aligns to 183:383/411 of A0QJI1

query
sites
A0QJI1

V

L

I

I

C

V

F

F

D
|
D

C

V

D
|
D

S

S

T

T

L

L

S

V

K

Q

I

G

E

E

G

V

I

I

D

E

E

M

L

L

A

A

G

A

R

K

V

A

G

G

L

A

G

E

E

G

E

Q

M

V

S

A

R

A

L

I

T

T

D

D

A

A

M

M

N

R

G

G

L

E

V

L

P

D

L

F

E

A

A

Q

V

S

Y

L

E

Q

K

Q

R

R

L

V

S

A

L

T

I

L

R

A

P

G

D

L

R

P

A

A

G

T

-

V

I

I

D

D

W

E

L

V

A

A

R

G

L

Q

Y

L

I

-

S

-

E

E

I

L

V

M

E

P

G

G

A

A

A

R

E

T

V

T

F

L

S

R

S

T

L

L

S

R

A

R

R

L

N

G

K

Y

E

A

L

C

H

G

I

V

S

S

G

G

I

F

R

R

Q

R

A

I

I

I

L

E

P

P

L

L

A

A

G

E

F

E

L

L

G

M

L

L

P

D

E

Y

S

V

R

A

V

A

H

N

A

E

V

L

D

E

V

I

Y

V

F

-

N

-

E

-

D

D

G

G

S

T

Y

L

R

T

G

G

Y

R

D

-

Q

V

S

V

S

G

P

P

L

I

A

I

R

D

T

R

G

A

G

G

K

K

A

A

-

T

-

A

-

L

-

R

E

E

I

F

C

A

R

Q

R

R

L

A

V

G

K

V

P

P

E

M

V

A

P

Q

L

T

L

V

M

A

I

V

G

G

D
|
D

G

G

K

A

T

N

D

D

M

I

E

D

A

M

K

L

Q

A

E

A

G

A

V

G

S

L

V

G

I

I

G

A

F

F

G

N

G

-

V

-

A

A

R

K

P

P

I

A

V

L

R

R

E

E

L

V

A

A

D

D

F

A

Y

S

F

L

D

S

H

H

P

P

T

Y

L

L

L

D

P

T

I

V

L

L

D187 (= D9) binding Mg(2+)
D189 (= D11) binding Mg(2+)
D343 (= D166) binding Mg(2+)

8a21A Crystal structure of phosphoserine phosphatase serb from mycobacterium avium in complex with phenylimidazole (see paper)
30% identity, 96% coverage: 5:209/213 of query aligns to 179:379/396 of 8a21A

query
sites
8a21A

V

L

I

I

C

V

F

F

D
|
D

C

V

D
|
D

S

S

T

T

L

L

S

V

K

Q

I

G

E
|
E

G
x
V

I
|
I

D

E

E

M

L

L

A

A

G

A

R

K

V

A

G

G

L

A

G

E

E

G

E

Q

M

V

S

A

R

A

L

I

T
|
T

D

D

A

A

M
|
M

N

R

G

G

L

E

V

L

P

D

L
x
F

E

A

A

Q

V

S

Y

L

E

Q

K

Q

R
|
R

L

V

S

A

L

T

I

L

R

A

P

G

D

L

R

P

A

A

G

T

-

V

I

I

D

D

W

E

L

V

A

A

R

G

L

Q

Y

L

I

-

S

-

E

E

I

L

V

M

E

P

G

G

A

A

A

R

E

T

V

T

F

L

S

R

S

T

L

L

S

R

A

R

R

L

N

G

K

Y

E

A

L

C

H

G

I

V

S

S

G

G

G
|
G

I

F

R

R

Q

R

A

I

I

I

L

E

P

P

L

L

A

A

G

E

F

E

L

L

G

M

L

L

P

D

E

Y

S

V

R

A

V

A

H

N

A

E

V

L

D

E

V

I

Y

V

F

-

N

-

E

-

D

D

G

G

S

T

Y

L

R

T

G

G

Y

R

D

-

Q

V

S

V

S

G

P

P

L

I

A

I

R

D

T

R

G

A

G

G

K

K

A

A

-

T

-

A

-

L

-

R

E

E

I

F

C

A

R

Q

R

R

L

A

V

G

K

V

P

P

E

M

V

A

P

Q

L

T

L

V

M

A

I

V

G

G

D
|
D

G

G

K

A

T

N

D

D

M

I

E

D

A

M

K

L

Q

A

E

A

G

A

V

G

S

L

V

G

I

I

G

A

F

F

G

N

G

-

V

-

A

A

R

K

P

P

I

A

V

L

R

R

E

E

L

V

A

A

D

D

F

A

Y

S

F

L

D

S

H

H

P

P

T

Y

L

L

L

D

P

T

I

V

L

L

binding magnesium ion: D183 (= D9), D185 (= D11), D339 (= D166)
binding 4-phenyl-1h-imidazole: D185 (= D11), E192 (= E18), V193 (≠ G19), I194 (= I20), T211 (= T37), M215 (= M41), F221 (≠ L47), R228 (= R54), G273 (= G100)

Sites not aligning to the query:

binding 4-phenyl-1h-imidazole: 13, 18, 37

8a1zA Crystal structure of phosphoserine phosphatase serb from mycobacterium avium in complex with 1-(2,4-dichlorophenyl)-3-hydroxyurea (see paper)
30% identity, 96% coverage: 5:209/213 of query aligns to 179:379/396 of 8a1zA

query
sites
8a1zA

V

L

I

I

C

V

F

F

D
|
D

C

V

D
|
D

S

S

T

T

L

L

S

V

K

Q

I

G

E
|
E

G

V

I

I

D

E

E

M

L

L

A

A

G

A

R

K

V

A

G

G

L

A

G

E

E

G

E

Q

M

V

S

A

R

A

L

I

T

T

D

D

A

A

M
|
M

N

R

G

G

L

E

V

L

P

D

L
x
F

E

A

A

Q

V

S

Y
x
L

E

Q

K

Q

R
|
R

L

V

S

A

L

T

I

L

R

A

P

G

D

L

R

P

A

A

G

T

-

V

I

I

D

D

W

E

L

V

A

A

R

G

L

Q

Y

L

I

-

S

-

E

E

I

L

V

M

E

P

G

G

A

A

A

R

E

T

V

T

F

L

S

R

S

T

L

L

S

R

A

R

R

L

N

G

K

Y

E

A

L

C

H

G

I

V

S

S

G
|
G

G

G

I
x
F

R

R

Q

R

A

I

I

I

L

E

P

P

L

L

A

A

G

E

F

E

L

L

G

M

L

L

P

D

E

Y

S

V

R

A

V

A

H

N

A

E

V

L

D

E

V

I

Y

V

F

-

N

-

E

-

D

D

G

G

S

T

Y

L

R

T

G

G

Y

R

D

-

Q

V

S

V

S

G

P

P

L

I

A

I

R

D

T

R

G

A

G

G

K

K

A

A

-

T

-

A

-

L

-

R

E

E

I

F

C

A

R

Q

R

R

L

A

V

G

K

V

P

P

E

M

V

A

P

Q

L

T

L

V

M

A

I

V

G

G

D
|
D

G

G

K

A

T

N

D

D

M

I

E

D

A

M

K

L

Q

A

E

A

G

A

V

G

S

L

V

G

I

I

G

A

F

F

G

N

G

-

V

-

A

A

R

K

P

P

I

A

V

L

R

R

E

E

L

V

A

A

D

D

F

A

Y

S

F

L

D

S

H

H

P

P

T

Y

L

L

L

D

P

T

I

V

L

L

binding 1-(2,4-dichlorophenyl)-3-oxidanyl-urea: D185 (= D11), E192 (= E18), M215 (= M41), F221 (≠ L47), L225 (≠ Y51), R228 (= R54), G272 (= G99), F274 (≠ I101), D339 (= D166)
binding magnesium ion: D183 (= D9), D185 (= D11), D339 (= D166)

O53289 Phosphoserine phosphatase SerB2; PSP; PSPase; O-phosphoserine phosphohydrolase; Protein-serine/threonine phosphatase; EC 3.1.3.3; EC 3.1.3.16 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
30% identity, 96% coverage: 5:209/213 of query aligns to 181:381/409 of O53289

query
sites
O53289

V

L

I

I

C

V

F

F

D
|
D

C
x
V

D
|
D

S
|
S

T

T

L

L

S

V

K

Q

I

G

E

E

G

V

I

I

D

E

E

M

L

L

A

A

G

A

R

R

V

A

G

G

L

A

G

Q

E

G

E

Q

M

V

S

A

R

A

L

I

T

T

D

E

A

A

M

M

N

R

G

G

L

E

V

L

P

D

L

F

E

A

A

E

V

S

Y

L

E

Q

K

R

R

R

L

V

S

A

L

T

I

L

R

A

P

G

D

L

R

P

A

A

G

T

-

V

I

I

D

D

W

D

L

V

A

A

R

E

L

Q

Y

L

I

-

S

-

E

E

I

L

V

M

E

P

G

G

A

A

A

R

E

T

V

T

F

I

S

R

S

T

L

L

S

R

A

R

R

L

N

G

K

F

E

R

L

C

H

G

I

V

S
|
S

G

G

I

F

R

R

Q

R

A

I

I

I

L

E

P

P

L

L

A

A

G

R

F

E

L

L

G

M

L

L

P

D

E

F

S

V

R

A

V

S

H

N

A

E

V

L

D

E

V

I

Y

V

F

-

N

-

E

-

D

D

G

G

S

I

Y

L

R

T

G

G

Y

R

D

-

Q

V

S

V

S

G

P

P

L

I

A

V

R

D

T

R

G

P

G

G

K
|
K

A

A

E

K

I

A

C

L

R

R

R

D

L

F

V

A

K

S

-

Q

-

Y

-

G

-

V

P

P

E

M

V

E

P

Q

L

T

L

V

M

A

I

V

G

G

D
|
D

G

G

K

A

T

N

D
|
D

M

I

E

D

A

M

K

L

Q

G

E

A

G

A

V

G

S

L

V

G

I

I

G

A

F

F

G

N

G

-

V

-

A

A

R

K

P

P

I

A

V

L

R

R

E

E

L

V

A

A

D

D

F

A

Y

S

F

L

D

S

H

H

P

P

T

Y

L

L

L

D

P

T

I

V

L

L

D185 (= D9) mutation to G: Completely abolishes enzymatic activity.; mutation to N: Completely abolishes enzymatic activity.
V186 (≠ C10) mutation to Q: Decreases enzymatic activity by 50%.
D187 (= D11) mutation to N: Decreases enzymatic activity by 15%.
S188 (= S12) mutation to A: No effect on enzymatic activity.
S273 (= S98) mutation to A: Completely abolishes enzymatic activity (PubMed:25521849). Decreases enzymatic activity by 60% (PubMed:25037224).
K318 (= K147) mutation to A: Decreases enzymatic activity by 50%.; mutation to E: Completely abolishes enzymatic activity.
D341 (= D166) mutation to G: Decreases enzymatic activity by 80%.; mutation to N: Decreases enzymatic activity by 85%. Completely abolishes enzymatic activity, does not elicit cytoskeletal rearrangements, and does not suppress IL-8 production after TNF-alpha stimulation; when associated with N-345.
D345 (= D170) mutation to N: Decreases enzymatic activity by 55%. Completely abolishes enzymatic activity, does not elicit cytoskeletal rearrangements, and does not suppress IL-8 production after TNF-alpha stimulation; when associated with N-341.

Sites not aligning to the query:

18 G→A: Does not bind L-serine and correspondingly no oligomeric transitions is observed in the presence of L-serine.
108 G→A: Does not bind L-serine and correspondingly no oligomeric transitions is observed in the presence of L-serine.

7qplA Crystal structure of phosphoserine phosphatase (serb) from brucella melitensis in complex with phosphate and magnesium
26% identity, 96% coverage: 5:209/213 of query aligns to 82:283/295 of 7qplA

query
sites
7qplA

V

I

I

L

C

I

F

A

D
|
D

C

M

D
|
D

S

S

T

T

L

M

S

I

K

G

I

Q

E

E

G

C

I

I

D

D

E

E

L

L

A

A

G

E

R

E

V

A

G

G

L

L

G

R

E

D

E

H

M

V

S

A

R

A

L

I

T

T

D

A

A

R

A

A

M

M

N

N

G

G

L

E

V

I

P

A

L

F

E

E

A

P

V

A

Y

L

E

R

K

E

R

R

L

V

S

A

L

L

I

L

R

K

P

G

-

L

D

P

R

L

A

S

G

V

I

I

D

D

W

K

L

V

A

I

R

S

L

T

Y

R

I

I

S

T

E

-

I

L

V

T

E

P

G

G

A

G

A

P

E

Q

V

L

F

V

S

R

S

T

L

M

S

R

A

K

R

H

N

G

K

A

E

Y

L

T

H

A

I

L

I

V

S

S

G

G

I

F

R

T

Q

S

A

F

I

T

L

R

P

R

L

I

A

A

G

E

F

M

L

I

G

G

L

F

P

N

E

E

S

E

R

R

V

A

H

N

A

-

V

-

D

-

V

-

Y

R

F

L

N

I

E

D

D

D

G

G

S

T

Y

R

R

L

G

T

Y

G

D

T

Q

V

S

A

S

E

P

P

L

I

-

L

-

G

-

R

-

E

A

A

R

K

T

V

G

E

G

K

K

L

A

V

E

E

I

I

C

A

R

E

R

R

L

V

-

G

V

L

K

T

P

P

E

E

V

-

P

D

L

A

L

I

M

A

I

V

G

G

D
|
D

G

G

K

A

T

N

D

D

M

L

E

-

A

-

K

-

Q

-

E

-

G

G

V

M

S

I

V

Q

I

L

G

A

F

G

G

T

G

G

V

V

V

A

-

L

-

H

A

A

R

K

P

P

I

A

V

V

R

A

E

A

L

Q

A

A

D

K

F

M

Y

R

F

I

D

D

H

H

P

G

T

D

L

L

L

T

P

A

I

L

L

L

binding magnesium ion: D86 (= D9), D88 (= D11), D243 (= D166)

Query Sequence

>WP_020565838.1 NCBI__GCF_000372865.1:WP_020565838.1
MSFDVICFDCDSTLSKIEGIDELAGRVGLGEEMSRLTDAAMNGLVPLEAVYEKRLSLIRP
DRAGIDWLARLYISEIVEGAAEVFSSLSARNKELHIISGGIRQAILPLAGFLGLPESRVH
AVDVYFNEDGSYRGYDQSSPLARTGGKAEICRRLVKPEVPLLMIGDGKTDMEAKQEGVSV
IGFGGVVARPIVRELADFYFDHPTLLPILSYIL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory