SitesBLAST

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
35% identity, 57% coverage: 42:137/168 of query aligns to 84:184/203 of P07464

query
sites
P07464

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N85 (≠ S43) binding in other chain; binding in other chain
D93 (= D51) binding substrate
H115 (≠ A71) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (≠ A95) binding in other chain
A160 (≠ S113) binding in other chain
TK 165:166 (= TK 118:119) binding acetyl-CoA
R180 (≠ K133) binding acetyl-CoA
R183 (= R136) binding in other chain

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; Partial
17 binding substrate
71 binding substrate

8j40A Crystal structure of catb8 in complex with chloramphenicol (see paper)
52% identity, 31% coverage: 85:136/168 of query aligns to 110:161/209 of 8j40A

query
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8j40A

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binding chloramphenicol: W117 (≠ F92), V135 (= V110), S138 (= S113)

Sites not aligning to the query:

binding chloramphenicol: 7, 8, 9, 29, 31, 47, 50, 90, 187, 190, 191, 202, 203, 206

4n6bA Soybean serine acetyltransferase complexed with coa (see paper)
33% identity, 63% coverage: 31:135/168 of query aligns to 129:233/233 of 4n6bA

query
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4n6bA

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binding coenzyme a: H150 (≠ N54), L169 (= L73), G176 (= G80), K208 (≠ V110), G210 (= G112), A211 (≠ S113), L216 (≠ T118), V226 (≠ A128)

4mzuB Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
37% identity, 46% coverage: 57:134/168 of query aligns to 64:139/290 of 4mzuB

query
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4mzuB

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binding coenzyme a: F72 (≠ Y65), T80 (≠ L73), N81 (≠ T74), D82 (≠ H75), K83 (≠ D76), P85 (≠ T78), G99 (= G94), A100 (= A95), G117 (= G112), A118 (≠ S113), T123 (= T118), K124 (= K119), I133 (≠ A128), R138 (≠ K133)
binding magnesium ion: N76 (≠ E69)

Sites not aligning to the query:

binding coenzyme a: 199
binding magnesium ion: 58
binding thymine: 20, 21, 38, 39
binding thymidine-5'-diphosphate: 171, 176, 189, 191, 202, 203, 270

4mzuF Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
37% identity, 46% coverage: 57:134/168 of query aligns to 64:140/294 of 4mzuF

query
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4mzuF

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binding coenzyme a: N81 (≠ H75), D82 (= D76), K83 (≠ L77), G100 (= G94), A101 (= A95), L106 (= L100), G118 (= G112), A119 (≠ S113), T124 (= T118), K125 (= K119), I134 (≠ A128), N136 (= N130), R139 (≠ K133)

Sites not aligning to the query:

binding thymine: 20, 21, 38
binding thymidine-5'-diphosphate: 189, 203, 205, 270

4n69A Soybean serine acetyltransferase complexed with serine (see paper)
32% identity, 63% coverage: 31:135/168 of query aligns to 136:243/243 of 4n69A

query
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4n69A

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binding serine: G183 (= G80), R191 (≠ L83), H192 (= H84)

3fsbA Crystal structure of qdtc, the dtdp-3-amino-3,6-dideoxy-d-glucose n- acetyl transferase from thermoanaerobacterium thermosaccharolyticum in complex with coa and dtdp-3-amino-quinovose (see paper)
41% identity, 46% coverage: 68:144/168 of query aligns to 150:228/260 of 3fsbA

query
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3fsbA

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binding coenzyme a: F150 (= F68), A178 (≠ G94), A179 (= A95), G196 (= G112), A197 (≠ S113), V212 (≠ A128)
binding [(3R,4S,5S,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: F150 (= F68)

Sites not aligning to the query:

binding [(3R,4S,5S,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: 78, 114, 132, 241, 243

3fscA Crystal structure of qdtc, the dtdp-3-amino-3,6-dideoxy-d-glucose n- acetyl transferase from thermoanaerobacterium thermosaccharolyticum in complex with coa and dtdp-3-amino-fucose (see paper)
41% identity, 46% coverage: 68:144/168 of query aligns to 150:228/259 of 3fscA

query
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3fscA

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binding coenzyme a: F150 (= F68), A178 (≠ G94), A179 (= A95), G196 (= G112), A197 (≠ S113), V212 (≠ A128), K228 (≠ R144)

Sites not aligning to the query:

binding (3R,4S,5R,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: 78, 112, 114, 115, 132, 133, 243

3fs8A Crystal structure of qdtc, the dtdp-3-amino-3,6-dideoxy-d-glucose n- acetyl transferase from thermoanaerobacterium thermosaccharolyticum in complex with acetyl-coa (see paper)
41% identity, 46% coverage: 68:144/168 of query aligns to 150:228/259 of 3fs8A

query
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3fs8A

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binding acetyl coenzyme *a: F150 (= F68), P151 (≠ E69), A178 (≠ G94), A179 (= A95), G196 (= G112), A197 (≠ S113), V212 (≠ A128), K228 (≠ R144)

Sites not aligning to the query:

binding thymine: 84, 102

4h7oA Crystal structure of serine acetyltransferase from vibrio cholerae o1 biovar el tor n16961
37% identity, 60% coverage: 36:135/168 of query aligns to 138:240/258 of 4h7oA

query
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4h7oA

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binding cysteine: D153 (= D51), H154 (≠ K52), G180 (≠ L81), R188 (≠ L83), H189 (= H84)

Sites not aligning to the query:

binding arginine: 51, 53, 127, 131
binding cysteine: 88, 89

6wyeA Crystal structure of neisseria gonorrhoeae serine acetyltransferase (cyse) (see paper)
32% identity, 65% coverage: 31:140/168 of query aligns to 138:250/261 of 6wyeA

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6wyeA

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K

K

I

P

I

V

R

A

S

R

D

S

I

L

K

K

binding (2S)-2-hydroxybutanedioic acid: D158 (= D51), H159 (≠ K52), G185 (vs. gap), R193 (vs. gap), H194 (= H84)

Sites not aligning to the query:

binding (2S)-2-hydroxybutanedioic acid: 93, 94

7ra4A Crystal structure of neisseria gonorrhoeae serine acetyltransferase (cyse) in complex with serine (see paper)
33% identity, 61% coverage: 31:133/168 of query aligns to 136:241/243 of 7ra4A

query
sites
7ra4A

T

S

K

E

F

V

W

F

G

G

M

V

D

D

I

I

D

H

P

P

T

A

A

A

S

R

F

L

S

G

L

Y

S

G

V

L

R

M

F

L

D
|
D

K
x
H

T

-

N

-

P

-

K

-

G

-

L

-

H

-

I

-

G

-

A

A

E

T

T

G

Y

F

V

V

A

A

F

G

E

E

A

T

A

A

I

V

L

L

T

G

H

N

D

N

L

I

T

S

-

I

-

L

R

H

G

G

L

V

Y

T

L

L

-

G

-

S

-

G

-

K

-

E

-

G

-

D

-
x
R

H
|
H

T

P

R

K

I

I

G

G

K

D

R

G

C

V

F

M

I

I

G

G

A

A

R

N

S

A

I

S

I

I

L

L

P

G

G

N

V

I

E

R

I

I

G

G

D

S

E

N

C

A

V

K

I

I

G

G

S

A

G

G

S

S

V

V

T

V

K

S

S

D

V

V

P

P

R

S

S

I

L

T

V

V

A

V

G

G

N

V

P

P

A

A

K

K

binding serine: D156 (= D51), H157 (≠ K52), R191 (vs. gap), H192 (= H84)

Sites not aligning to the query:

binding serine: 91, 92

8vr6A Crystal structure of the pcryo_0619 n-acetryltransferase from psychrobacter cryohalolentis k5 in the presence of coa-disulfide
49% identity, 30% coverage: 85:135/168 of query aligns to 118:168/177 of 8vr6A

query
sites
8vr6A

T

T

R

R

I

I

G

G

K

S

R

D

C

C

F

Y

I

L

G

G

A

P

R

N

S

T

I

I

L
x
V

P

K

G

G

V

V

E

K

I

I

G

G

D

D

E

R

C

V

V

V

I

V

G

G

S

A

G

N

S

S

V
x
L

V

V

T
x
L

K

K

S

D

V

I

P

P

P

S

R

D

S

C

L

K

V

V

A

F

G

G

N
x
S

P
|
P

A

A

K

V

I

I

binding [[(2~{S},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(3~{R})-4-[[3-[2-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyldisulfanyl]ethylamino]-3-oxidanylidene-propyl]amino]-2,2-dimethyl-3-oxidanyl-4-oxidanylidene-butyl] hydrogen phosphate: V133 (≠ L100), L149 (≠ V116), L151 (≠ T118), S163 (≠ N130), P164 (= P131)

Sites not aligning to the query:

binding [[(2~{S},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(3~{R})-4-[[3-[2-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyldisulfanyl]ethylamino]-3-oxidanylidene-propyl]amino]-2,2-dimethyl-3-oxidanyl-4-oxidanylidene-butyl] hydrogen phosphate: 97, 99, 100, 101

8vr7E Crystal structure of the pcryo_0619 n-acetyltransferase from psychrobacter cryohalolentis k5 int he presence of acetyl coenzyme a
49% identity, 30% coverage: 85:135/168 of query aligns to 119:169/180 of 8vr7E

query
sites
8vr7E

T

T

R

R

I

I

G

G

K

S

R

D

C

C

F
x
Y

I

L

G

G

A

P

R

N

S

T

I

I

L
x
V

P

K

G

G

V

V

E

K

I

I

G

G

D

D

E

R

C

V

V

V

I

V

G
|
G

S
x
A

G

N

S

S

V
x
L

V

V

T
x
L

K

K

S

D

V

I

P

P

P

S

R

D

S

C

L
x
K

V

V

A
x
F

G

G

N
x
S

P
|
P

A

A

K

V

I

I

binding acetyl coenzyme *a: Y126 (≠ F92), V134 (≠ L100), G146 (= G112), A147 (≠ S113), L150 (≠ V116), L152 (≠ T118), K160 (≠ L126), F162 (≠ A128), S164 (≠ N130), P165 (= P131)

Sites not aligning to the query:

binding acetyl coenzyme *a: 69, 92, 98, 101, 102, 175
binding 3'-phosphate-adenosine-5'-diphosphate: 24, 26, 37, 38

Query Sequence

>WP_020922035.1 NCBI__GCF_000092045.1:WP_020922035.1
MVQTGKKPKWGLNVFRPLRNGIVKAKWLYYTKFWGMDIDPTASFSLSVRFDKTNPKGLHI
GAETYVAFEAAILTHDLTRGLYLHTRIGKRCFIGARSIILPGVEIGDECVIGSGSVVTKS
VPPRSLVAGNPAKIIRSDIKIISRYGRMAREDETVTQLVTHLPTGEAR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory