SitesBLAST

T27 (= T31) mutation T->A,S: Retains 60% of wild-type activity.; mutation to N: Retains 20% of wild-type activity.
E98 (= E102) mutation to A: Retains 80% of wild-type activity.
K156 (= K160) mutation K->A,Q: Retains less than 10% of wild-type activity.; mutation to R: Retains 40% of wild-type activity.
F212 (= F214) mutation F->A,Q: Loss of activity.; mutation to Y: No change in activity.
E218 (= E220) mutation E->A,Q: Loss of activity.; mutation to D: Retains 70% of wild-type activity.

P24207 Phenylalanine-specific permease; Phenylalanine:H(+) symporter PheP from Escherichia coli (strain K12) (see 3 papers)
45% identity, 96% coverage: 2:447/463 of query aligns to 10:452/458 of P24207

query
sites
P24207

N

D

S

T

D

A

A

S

A

N

T

Q

Q

E

D

P

G

T

L

L

K

H

R

R

G

G

L

L

T

H

A

N

R
|
R

H

H

I

I

R

Q

F

L

I

I

A

A

L

L

G

G

S

G

A

A

I

I

G

G

T

T

G

G

L

L

F

F

Y

L

G

G

S

I

A

G

E

P

A

A

I

I

K

Q

R

M

A

A

G

G

P
|
P

S

A

V

V

L

L

A

G

Y

Y

L

G

I

V

G

A

G

G

I

I

A

I

V

A

Y

F

L

L

V

I

L

M

R

R

A

Q

L

L

G

G

E

E

M

M

A

V

V

V

R

E

N

E

P

P

V

V

S

S

G

G

S

S

F
|
F

S

A

E

H

Y
x
F

A

A

N
x
Y

K

K

H
x
Y

L
x
W

G

G

P

P

L
x
F

A

A

G

G

F
|
F

M

L

T

S

G

G

W
|
W

T

N

Y
|
Y

T
x
W

F

V

E

M

M
x
F

V

V

I

L

V

V

C

G

L

M

A

A

D
x
E

V

L

T

T

A

A

F

A

G

G

L

I

Y

Y

M

M

Q

Q

F

Y

W

W

F

F

P

P

D

D

V

V

P

P

R

T

W

W

I

I

W

W

V

A

L

A

A

A

V

F

F

I

I

I

G

N

A

A

I

V

N

N

L

L

L

V

S

N

V

V

K

R

V

L

F

Y

G

G

E

E

L

T

E

E

F

F

W

W

F

F

T

A

L

L

V

I

K
|
K

I

V

T

L

A

A

I

I

A

G

M

M

I

I

A

G

G

F

G

G

I

L

A

W

I

L

V

F

F

S

G

G

F

H

G

G

V

-

H

-

D

G

T

E

D

K

A

A

G

S

I

I

S

D

H

N

L

L

W

W

S

R

D

Y

G

G

F

F

A

A

T

T

G

G

F

W

G

N

G

G

F

L

V

I

A

L

C

S

F

L

A

A

I

V

V

I

M

M

F

F

A

S

F

F

G

G

T

L

E
|
E

I

L

I

I

G

G

I

I

T

T

A

A

G

A

E

E

A

A

E

R

D

D

P

P

A

E

Q

K

T

S

I

I

R

P

K

K

A

A

V

V

N

N

T

Q

V

V

P

V

V

Y

R
|
R

I

I

I

L

L

L

F

F

Y

Y

I

I

C

G

T

S

L

L

A

V

V

V

I

L

M

L

A

A

I

L

I

Y

P

P

W

W

Q

V

T

E

I

V

N

K

S

S

D

N

N

S

S

S

P

P

F

F

V

V

Q

M

I

I

F

F

E

H

N

N

L

L

G

D

L

S

G

N

T

V

A

V

A

A

S

S

I

A

L

L

N

N

I

F

V

V

V

I

I

L

T

V

A

A

A

S

L

L

S

S

A

V

I

Y

N

N

S

S

D

G

V

V

F

Y

G

S

A

N

G

S

R

R

M

M

M

L

F

F

G

G

M

L

S

S

H

V

A

Q

G

G

Q

N

A

A

P

P

Q

K

V

F

M

L

K

T

R

R

V

V

S

S

A

R

N

R

G

G

V

V

P
|
P

W

I

M

N

T

S

V

L

V

M

I

L

M

S

T

G

V

A

A

I

L

T

L

S

V

L

G

V

V

V

V

L

L

I

N

N

Y

Y

L

L

I

L

P

P

D

Q

Q

K

V

A

F

F

L

G

V

L

I

L

A

M

S

A

L

L

A

V

T

V

F

A

A

T

T

L

I

L

F

L

V

N

W

W

I

I

M

M

I

I

L

C

L

L

S

A

Q

H

F

L

R

R

S

F

R

R

A

A

Q

A

M

M

S

R

A

-

D

R

E

Q

T

G

A

R

A

E

L

T

K

Q

F

F

P

K

V

A

P

L

L

L

W

Y

P

P

Y

F

G

G

Q

N

I

Y

F

L

A

C

I

I

V

A

F

F

L

L

A

G

F

M

V

I

I

L

V

L

L

L

L

M

G

C

V

T

I

M

A

D

D

D

T

M

R

R

V

L

A

S

L

A

L

I

V

L

G

L

A
x
P

G

V

W

W

L

I

V

V

L

F

L

L

T

F

G

M

A

A

Y

F

R26 (= R18) mutation R->G,S,Q: Strong decrease in phenylalanine transport activity.
P54 (= P46) mutation to A: 50% of wild-type phenylalanine transport activity.; mutation to G: No change in phenylalanine transport activity.; mutation to L: 26% of wild-type phenylalanine transport activity.
F87 (= F79) mutation to L: No effect on phenylalanine transport activity.
F90 (≠ Y82) mutation to L: 65% of wild-type phenylalanine transport activity.
Y92 (≠ N84) mutation to L: 41% of wild-type phenylalanine transport activity.
Y94 (≠ H86) mutation to L: 69% of wild-type phenylalanine transport activity.
W95 (≠ L87) mutation to L: 10% of wild-type phenylalanine transport activity.
F98 (≠ L90) mutation to L: No effect on phenylalanine transport activity.
F101 (= F93) mutation to L: 38% of wild-type phenylalanine transport activity.
W105 (= W97) mutation to L: 39% of wild-type phenylalanine transport activity.
Y107 (= Y99) mutation to L: No effect on phenylalanine transport activity.
W108 (≠ T100) mutation to L: 71% of wild-type phenylalanine transport activity.
F111 (≠ M103) mutation to L: 60% of wild-type phenylalanine transport activity.; mutation to Y: Enables the transport of tryptophan to almost the same steady-state level as that of phenylalanine.
E118 (≠ D110) mutation E->G,L,V,N: Loss of activity.
K168 (= K160) mutation K->L,R: Strong decrease in phenylalanine transport activity.; mutation to N: Loss of activity.
E226 (= E220) mutation E->A,Q,K,R,W: Loss of activity.
R252 (= R246) mutation R->D,E,F,W,P: Loss of activity.
P341 (= P335) mutation to A: 5% of wild-type phenylalanine transport activity.; mutation P->G,Q,K,R: Loss of activity.; mutation to S: 3% of wild-type phenylalanine transport activity.; mutation to T: 17% of wild-type phenylalanine transport activity.
P442 (≠ A437) mutation to A: 46% of wild-type phenylalanine transport activity.; mutation to G: 52% of wild-type phenylalanine transport activity.; mutation to L: 43% of wild-type phenylalanine transport activity.

P15993 Aromatic amino acid transport protein AroP; Aromatic amino acid:H(+) symporter AroP; General aromatic amino acid permease; General aromatic transport system from Escherichia coli (strain K12) (see paper)
44% identity, 95% coverage: 9:447/463 of query aligns to 9:444/457 of P15993

query
sites
P15993

D

E

G

Q

L

L

K

K

R

R

G

G

L

L

T

K

A

N

R

R

H

H

I

I

R

Q

F

L

I

I

A

A

L

L

G

G

S

G

A

A

I

I

G

G

T

T

G

G

L

L

F

F

Y

L

G

G

S

S

A

A

E

S

A

V

I

I

K

Q

R

S

A

A

G

G

P

P

S

G

V

I

L

I

L

L

A

G

Y

Y

L

A

I

I

G

A

G

G

I

F

A

I

V

A

Y

F

L

L

V

I

L

M

R

R

A

Q

L

L

G

G

E

E

M

M

A

V

V

V

R

E

N

E

P

P

V

V

S

A

G

G

S

S

F

F

S

S

E

H

Y

F

A

A

N

Y

K

K

H

Y

L

W

G

G

P

S

L

F

A

A

G

G

F

F

M

A

T

S

G

G

W

W

T

N

Y

Y

T

W

F

V

E

L

M
x
Y

V

V

I

L

V

V

C

A

L

M

A

A

D

E

V

L

T

T

A

A

F

V

G

G

L

K

Y

Y

M

I

Q

Q

F

F

W

W

F

Y

P

P

D

E

V

I

P

P

R

T

W

W

I

-

W

-

V

V

L

S

A

A

V

A

V

V

F

F

-

V

I

I

G

N

A

A

I

I

N

N

L

L

L

T

S

N

V

V

K

K

V

V

F

F

G

G

E

E

L

M

E

E

F

F

W

W

F

F

T

A

L

I

V

I

K

K

I

V

T

I

A

A

I

V

A

A

M

M

I

I

A

I

G

F

G

G

I

G

A

W

I

L

V

F

F

S

G

G

F

N

G

G

V

-

H

-

D

G

T

P

D

Q

A

A

G

T

I

V

S

S

H

N

L

L

W

W

S

D

D

Q

G

G

F

F

F

L

A

P

T

H

G

G

F

F

G

T

G

G

F

L

V

V

A

M

C

M

F

M

A

A

I

I

V

I

M

M

F

F

A

S

F

F

G

G

T

L

E

E

I

L

I

V

G

G

I

I

T

T

A

A

G

A

E

E

A

A

E

D

D

N

P

P

A

E

Q

Q

T

S

I

I

R

P

K

K

A

A

V

T

N

N

T

Q

V

V

P

I

V

Y

R

R

I

I

I

L

L

I

F

F

Y

Y

I

I

C

G

T

S

L

L

A

A

V

V

I

L

M

L

A

S

I

L

I

M

P

P

W

W

Q

T

T

R

I

V

N

T

S

A

D

D

N

T

S

S

P

P

F

F

V

V

Q

L

I

I

F

F

E

H

N

E

L

L

G

G

L

D

G

T

T

F

A

V

A

A

S

N

I

A

L

L

N

N

I

I

V

V

I

L

T

T

A

A

L

L

S

S

A

V

I

Y

N

N

S

S

D

C

V

V

F

Y

G

C

A

N

G

S

R

R

M

M

M

L

F

F

G

G

M

L

S

A

H

Q

A

Q

G

G

Q

N

A

A

P

P

Q

K

V

A

M

L

K

A

R

S

V

V

S

D

A

K

N

R

G

G

V

V

P

P

W

V

M

N

T

T

V

I

V

L

I

V

M

S

T

A

V

L

A

V

L

T

L

A

V

L

G

C

V

V

V

L

L

I

N

N

Y

Y

L

L

I

A

P

P

D

E

Q

S

V

A

F

F

L

G

V

L

I

L

A

M

S

A

L

L

A

V

T

V

F

S

A

A

T

L

I

V

F

I

V

N

W

W

I

A

M

M

I

I

L

S

L

L

S

A

Q

H

F

M

R

K

S

F

R

R

A

-

Q

R

M

A

S

K

A

Q

D

E

E

Q

T

G

A

V

L

T

K

R

F

F

P

P

V

A

P

L

L

L

W

Y

P

P

Y

L

G

G

Q

N

I

W

F

I

A

C

I

L

V

L

F

F

L

M

A

A

F

A

V

V

I

L

V

V

L

I

L

M

G

L

V

M

I

T

A

P

D

G

T

M

R

A

V

I

A

S

L

V

L

Y

V

L

G

I

A

P

G

V

W

W

L

L

V

I

L

V

L

L

T

G

G

I

A

G

Y

Y

Y103 (≠ M103) Key residue for tryptophan transport; mutation to F: Decreases tryptophan transport to less than 50% of wild-type levels and reduces the ability of tryptophan to inhibit phenylalanine transport from 95 to 62%.

P46349 Gamma-aminobutyric acid permease; GABA permease; 4-aminobutyrate permease; Gamma-aminobutyrate permease; Proline transporter GabP from Bacillus subtilis (strain 168) (see paper)
38% identity, 93% coverage: 7:435/463 of query aligns to 4:431/469 of P46349

query
sites
P46349

T

S

Q

Q

D

S

G

G

L

L

K

K

R

K

G

E

L

L

T

K

A

T

R

R

H

H

I

M

R

T

F

M

I

I

A

S

L

I

G

A

S

G

A

V

I

I

G

G

T

A

G

G

L

L

F

F

Y

V

G
|
G

S

S

A

G

E

S

A

V

I

I

K

H

R

S

A

T

G
|
G

P

P

S

G

V

A

L

V

A

S

Y

Y

L

A

I

L

G

A

G

G

I

L

A

L

V

V

Y

I

L

F

V

I

L

M

R

R

A

M

L

L

G

G

E

E

M

M

A

S

V

A

R

V

N

N

P

P

V

T

S

S

G

G

S

S

F

F

S

S

E

Q

Y

Y

A

A

N

H

K

D

H

A

L

I

G

G

P

P

L

W

A

A

G

G

F

F

M

T

T

I

G

G

W

W

T

L

Y

Y

T

W

F

F

E

F

M

W

V

V

I

I

V

V

C

I

L

A

A

I

D

E

V

A

T

I

A

A

F

G

G

A

L

G

Y

I

M

I

Q

Q

F

Y

W

W

F

F

P

H

D

D

V

I

P

P

R

L

W

W

I

L

W

T

V

S

L

L

A

I

V

L

V

T

F

I

F

V

I

L

G

T

A

L

I

T

N

N

L

V

L

Y

S

S

V

V

K

K

V

S

F

F

G

G

E

E

L

F

E

E

F

Y

W

W

F

F

T

S

L

L

V

I

K

K

I

V

T

V

A

T

I

I

A

A

M

F

I

L

A

I

G

V

G

G

I

F

A

A

I

F

I

I

V

-

F

F

G

G

F

F

G

A

V

P

H

G

D

S

T

E

D

P

A

V

G

G

I

F

S

S

H

N

L

L

W

T

S

G

D

K

G

G

F

F

A

P

T

E

G

G

F

I

G

S

F

V

V

L

A

L

C

G

F

I

A

V

I

V

V

V

M

I

F

F

A

S

F

F

G

M

G

G

T

T

E

E

I

I

I

V

G

A

I

I

T

A

A

A

G

G

E

E

A

T

E

S

D

N

P

P

A

I

Q

E

T

S

I

V

R

T

K

K

A

A

V

T

N

R

T

S

V

V

P

V

V

W

R

R

I

I

L

V

F

F

Y

Y

I

V

C

G

T

S

L

I

A

A

V

I

I

V

M

V

A

A

I

L

P

P

W

W

Q

N

T

S

I

A

N

N

S

I

D

L

N

E

S

S

P

P

F

F

V

V

Q

A

I

V

F

L

E

E

N

H

L

I

G

G

L

V

G

P

T

A

A

A

S

Q

I

I

L

M

N

N

I

F

V

I

V

V

I

L

T

T

A

A

A

V

L

L

S

S

A

C

I

L

N

N

S

S

D
x
G

V

L

F

Y

G

T

A

T

G

S

R

R

M

M

M

L

F

Y

G

S

M

L

S

A

H

E

A

R

G

N

Q

E

A

A

P

P

Q

R

V

R

M

F

K

M

R

K

V

L

S

S

A

K

N

K

G

G

V

V

P

P

W

V

M

Q

T

A

V

I

V

V

I

A

M
x
G

T

T

V

F

A
x
F

L

S

L

Y

V

I

G

A

V

V

L

M

N

N

Y

Y

L

F

I

S

P

P

D

D

Q

T

V

V

F

F

L

L

V

F

I

L

A

V

S

N

L

S

A

S

T

G

F

A

A

I

T

A

I

L

F

L

V

V

W

Y

I

L

M

V

I

I

L

A

L

V

S

S

Q

Q

F

L

R

K

S

M

R

R

A

K

Q

K

M

L

S

E

A

K

D

T

E

N

T

P

A

E

A

A

L

L

K

K

F

I

P

K

V

M

P

W

L

L

W

F

P

P

Y

F

G

L

Q

T

I

Y

F

L

A

T

I

I

V

I

F

A

L

I

A

C

F
x
G

V

I

I

L

V

V

L

S

L

M

G

A

V

F

I

I

A

D

D

S

T

M

R

R

V

D

A

E

L

L

V

L

G33 (= G36) mutation to D: Lack of activity.
G42 (= G45) mutation to S: Lack of activity.
G301 (≠ D305) mutation to V: Lack of activity.
G338 (≠ M342) mutation to E: Lack of activity.
F341 (≠ A345) mutation to S: Lack of activity.
G414 (≠ F418) mutation to R: Lack of activity.

P25737 Lysine-specific permease LysP; Lysine transporter LysP; Trigger transporter LysP from Escherichia coli (strain K12) (see 2 papers)
37% identity, 92% coverage: 1:424/463 of query aligns to 1:434/489 of P25737

query
sites
P25737

M
|
M

N

V

S

S

D

E

A

T

A

K

T

T

Q

T

D

E

-

A

-

P

G

G

L

L

K

R

R

R

G

E

L

L

T

K

A

A

R

R

H

H

I

L

R

T

F

M

I

I

A

A

L

I

G

G

S

G

A

S

I

I

G

G

T

T

G

G

L

L

F

F

Y

V

G

A

S

S

A

G

E

A

A

T

I

I

K

S

R

Q

A

A

G

G

P

P

S

G

-

G

V

A

L

L

A

S

Y

Y

L

M

I

L

G

I

G

G

I

L

A

M

V

V

Y

Y

L

F

V

L

L

M

R

T

A

S

L

L

G

G

E

E

M

L

A

A

V

A

R

Y

N

M

P

P

V

V

S

S

G

G

S

S

F

F

S

A

E

T

Y

Y

A

G

N

Q

K

N

H

Y

L

V

G

E

P

E

L

G

A

F

G

G

F

F

M

A

T

L

G

G

W

W

T

N

Y
|
Y

T

W

F

Y

E

N

M
x
W

V

A

I

V

V

T

C

I

L

A

A

V

D

D

V

L

T

V

A

A

F

A

G

Q

L

L

Y

V

M

M

Q

S

F

W

W

W

F

F

P

P

D

D

V

T

P

P

R

G

W

W

I

I

W

W

V

S

L

A

A

L

V

F

V

L

F

G

F

V

I

I

G

F

A

L

I

L

N

N

L

Y

L

I

S

S

V

V

K

R

V

G

F

F

G

G

E

E

L

A

E

E

F

Y

W

W

F

F

T

S

L

L

V

I

K
|
K

I

V

T

T

A

T

I

V

I

I

A

V

M

F

I

I

A

I

G

V

G

G

I

V

A

L

I

M

I

I

V

I

F

-

G

G

F

I

G

F

V

K

H

G

D

A

T

Q

D

P

A

A

G

G

I

W

S

S

H

N

L

-

W

W

S

T

D

I

G

G

-

E

G

A

F

P

F

F

A

A

T

G

G

G

F

F

G

A

F

M

V

I

A

G

C

V

F

A

A

M

I

I

V

V

M

G

F
|
F

A

S

F

F

G

Q

G

G

T

T

E
|
E

I

L

I

I

G

G

I

I

T

A

A

A

G

G

E
|
E

A

S

E

E

D

D

P

P

A

A

Q

K

T

N

I

I

R

P

K

R

A

A

V

V

N

R

T

Q

V

V

P

F

V

W

R

R

I

I

I

L

L

L

F

F

Y

Y

I

V

C

F

T

A

L

I

A

L

V

I

I

I

M

S

A

L

I

I

P

P

W

Y

Q

T

T

D

-

P

-

S

-

L

I

L

N

R

S

N

D
|
D

-

V

-

K

-
x
D

-

I

-

S

N

V

S

S

P

P

F

F

V

T

Q

L

I

V

F

F

E

Q

N

H

L

A

G

G

L

L

G

L

T

S

A

A

S

A

I

V

L

M

N

N

I

A

V

V

V

I

I

L

T

T

A

A

A

V

L

L

S

S

A

A

I

G

N

N

S

S

D

G

V

M

F

Y

G

A

A

S

G

T

R

R

M

M

M

L

F

Y

G

T

M

L

S

A

H

C

A

D

G

G

Q

K

A

A

P

P

Q

R

V

I

M

F

K

A

R

K

V

L

S

S

A

R

N

G

G

G

V

V

P

P

-

R

-

N

-

A

-

L

W

Y

M

A

T

T

V

T

V

V

I

I

M

A

T

G

V

L

A

C

L

F

L

L

V

T

G

S

V

M

V

F

L

G

N

N

Y

-

L

-

I

-

P

-

D

Q

Q

T

V

V

F

Y

L

L

V

W

I

L

A

L

S

N

L

T

A

S

T

G

F

M

A

T

T

G

I

F

F

I

V

A

W

W

I

L

M

G

I

I

L

A

L

I

S

S

Q

H

F

Y

R

R

S

F

R

R

A

R

Q

G

-

Y

-

V

M

L

S

Q

A

G

D

H

E

D

T

I

A

N

A

D

L

L

K

P

F

Y

P

R

V

S

P

G

L

F

W

F

P

P

Y

L

G

G

Q

P

I

I

F

F

A

A

I

-

V

-

F

F

L

I

A

L

F

C

V

L

I

I

V

I

L

T

L

L

G

G

M1 (= M1) modified: Initiator methionine, Removed
Y102 (= Y99) mutation to L: Retains 4% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
W106 (≠ M103) mutation to L: Retains 20% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
K163 (= K160) mutation to A: Retains 24% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
F216 (= F214) mutation to L: Retains 13% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
E222 (= E220) mutation to A: Abolishes lysine uptake. Strongly inhibits CadC.
E230 (= E228) mutation to V: Abolishes lysine uptake. Shows significant less inhibition of CadC.
D275 (= D270) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-278.
D278 (vs. gap) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-275.

Sites not aligning to the query:

438 E→A: Retains 14% of wild-type lysine uptake activity. Is unable to inhibit CadC.
443 D→A: Retains 11% of wild-type lysine uptake activity. Is unable to inhibit CadC.
446 D→A: Retains 13% of wild-type lysine uptake activity. Is unable to inhibit CadC.

P04817 Arginine permease CAN1; Canavanine resistance protein 1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
33% identity, 90% coverage: 11:427/463 of query aligns to 84:517/590 of P04817

query
sites
P04817

L

V

K

K

R

R

G

E

L

L

T

K

A

Q

R

R

H

H

I

I

R

G

F

M

I

I

A

A

L

L

G

G

S

G

A

T

I

I

G

G

T

T

G

G

L

L

F

F

Y

I

G

G

S

L

A

S

E

T

A
x
P

I

L

K

T

R

N

A

A

G

G

P

P

-

V

S

G

V

A

L

L

L

I

A

S

Y

Y

L

L

I

F

G

M

G

G

I

S

A

L

V

A

Y

Y

L

S

V

V

L

T

R

Q

A

S

L

L

G

G

E

E

M

M

A

A

V

T

R

F

N

I

P
|
P

V
|
V

S

T

G

S

S
|
S

F

F

S

T

E

V

Y

F

A

S

N

Q

K

R

H

F

L

L

G

S

P

P

L

A

A

F

G

G

F

A

M

A

T

N

G

G

W

Y

T

M

Y
|
Y

T

W

F

F

E

S

M

W

V

A

I

I

V

T

C

F

L

A

A

L

D

E

V

L

T

S

A

V

F

V

G

G

L

Q

Y

V

M

I

Q

Q

F

F

W

W

F

T

P

Y

D

K

V

V

P

P

R

L

W

A

I

A

W

W

V

I

L

S

A

I

V

F

V

W

F

V

F

I

I

I

G

T

A

I

I

M

N

N

L

L

L

F

S

P

V

V

K

K

V

Y

F

Y

G

G

E

E

L

F

E

E

F

F

W

W

F

V

T

A

L

S

V

I

K

K

I

V

T

L

A

A

I

I

A

G

M

F

I

L

A

I

G

Y

G

C

I

F

A

C

I

M

I

V

V

C

F

-

G

G

F

A

G

G

V

V

H

-

D

-

T

T

D

G

A

P

G

V

I

G

S

F

H

R

L

Y

W

W

S

R

D

N

G

P

G

G

F

A

F

W

A

G

T

P

G

G

-

I

-

S

-

K

-

D

-

K

-

N

-

E

-

G

-

R

F

F

G

L

G

G

F

W

V

V

A

S

C

S

F

L

A

I

I

N

V

A

M

A

F

F

A

T

F

F

G

Q

G

G

T

T

E

E

I

L

I

V

G

G

I

I

T

T

A

A

G
|
G

E

E

A

A

E

A

D

N

P
|
P

A

R

Q

K

T

S

I

V

R

P

K

R

A

A

V

I

N

K

T

K

V

V

P

V

V

F

R

R

I

I

I

L

L

T

F

F

Y

Y

I

I

C

G

T

S

L

L

A

L

V

F

I

I

M

G

A

L

I

L

I

V

P

P

W

Y

Q

N

-

D

-

P

-

K

-

L

-

T

-

Q

-

S

T
|
T

I
x
S

N
x
Y

S

V

D

S

N

T

S

S

P

P

F

F

V

I

Q

I

I

A

F

I

E

E

N

N

L

S

G

G

L

T

G

K

T

V

A

L

A

P

S

H

I

I

L

F

N

N

I

A

V

V

V

I

I

L

T

T

A

T

A

I

L

I

S

S

A

A

I

A

N

N

S

S

D

N

V

I

F

Y

G

V

A

G

G

S

R

R

M

I

M

L

F

F

G

G

M

L

S

S

H

K

A

N

G

K

Q

L

A

A

P

P

Q

K

V

F

M

L

K

S

R

R

V

T

S

T

A

K

N

G

G

G

V

V

P

P

W

Y

M

I

T

A

V

V

V

F

I

V

M

T

T

A

V

A

A

F

L

G

L

A

V

L

G

A

V

Y

V

M

L

E

N

T

Y

S

L

T

I

G

P

G

D

D

Q

K

V

V

F

F

L

E

V
x
W

I

L

A

L

S

N

L

I

A

T

T

G

F

V

A

A

T

G

I
x
F

F

F

V

A

W

W

I

L

M

F

I

I

L

S

L

I

S

S

Q

H

F

I

R

R

-

F

-

M

-

Q

-

A

-

L

S

K

R

Y

A

R

Q

G

M

I

S

S

A

R

D

D

E

E

T

-

A

-

L

L

K

P

F

F

P

K

V

A

P

K

L

L

W

M

P

P

Y

G

G

L

Q

A

I

Y

F

Y

A

A

I

A

V

T

F

F

L

M

A

T

F

I

V

I

I

I

V

I

L

I

L

Q

G

G

V

F

I

T

A

A

P113 (≠ A40) mutation to L: In CAN1-343; confers citrulline transport activity in GAP1-deleted cells.
P148 (= P74) mutation to L: In CAN1-337; confers citrulline transport activity in GAP1-deleted cells and leads to sensitivity to L-glutamic acid alpha-hydroxamate, alpha-aminoisobutyrate, 3-chloro-L-alanine, L-ethionine, L-allylglycine, and D-histidine, but not sensitivity to L-aspartic acid alpha-hydroxamate or p-fluoro-L-phenylalanine.
V149 (= V75) mutation to F: In CAN1-315; confers citrulline transport activity in GAP1-deleted cells.
S152 (= S78) mutation to F: In CAN1-342; confers citrulline transport activity in GAP1-deleted cells.
Y173 (= Y99) mutation to D: In CAN1-306; confers citrulline transport activity in GAP1-deleted cells.; mutation to H: In CAN1-327; confers citrulline transport activity in GAP1-deleted cells.
G308 (= G227) mutation to A: In CAN1-341; confers citrulline transport activity in GAP1-deleted cells.
P313 (= P232) mutation to S: In CAN1-329; confers citrulline transport activity in GAP1-deleted cells and leads to sensitivity to L-glutamic acid alpha-hydroxamate, alpha-aminoisobutyrate, 3-chloro-L-alanine, L-ethionine, L-allylglycine, and D-histidine, L-aspartic acid alpha-hydroxamate and p-fluoro-L-phenylalanine.
TS 354:355 (≠ TI 266:267) mutation Missing: In CAN1-318; confers citrulline transport activity in GAP1-deleted cells.
Y356 (≠ N268) mutation to H: In CAN1-340; confers citrulline transport activity in GAP1-deleted cells.; mutation to N: In CAN1-339; confers citrulline transport activity in GAP1-deleted cells.
W451 (≠ V363) mutation to C: In CAN1-328; confers citrulline transport activity in GAP1-deleted cells.; mutation to L: In CAN1-316; confers citrulline transport activity in GAP1-deleted cells.; mutation to S: In CAN1-335; confers citrulline transport activity in GAP1-deleted cells.
F461 (≠ I373) mutation to S: In CAN1-307; confers citrulline transport activity in GAP1-deleted cells.

Q9URZ4 Cationic amino acid transporter 1 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
31% identity, 89% coverage: 3:415/463 of query aligns to 70:496/587 of Q9URZ4

query
sites
Q9URZ4

S

A

D

K

A

A

A

T

T

G

Q

E

D

D

G

G

-

T

-

A

L

L

K

K

R

R

G

S

L

L

T

K

A

S

R

R

H

H

I

M

R

Q

F

M

I

I

A

S

L

I

G

G

S

G

A

A

I

I

G

G

T

T

G

G

L

L

F

Y

Y

V

G

G

S

S

A

G

E

S

A

S

I

L

K

A

R

D

A

G

G

G

P

P

-

A

S

S

V

V

L

I

A

N

Y

Y

L

S

I

L

G

I

G

G

I

I

A

M

V

M

Y

F

L

F

V

I

L

V

R

Y

A

A

L

L

G

G

E

E

M

M

A

A

V

V

R

A

N

Y

P

P

V

V

S

A

G

G

S

G

F

F

S

N

E

T

Y

Y

A

A

N

T

K

R

H

F

L

I

G

D

P

P

L

A

A

W

G

G

F

F

M

A

T

V

G

S

W

W

T

N

Y

Y

T

F

F

I

E

N

M

Y

V

F

I

V

V

T

C

F

L

P

A

L

D

E

V

L

T

T

A

T

F

C

G

A

L

I

Y

T

M

F

Q

R

F

Y

W

W

F

-

P

T

D

D

V

I

P

N

R

S

W

A

I

A

W

W

V

I

L

S

A

I

V

F

V

L

F

V

I

I

G

I

A

I

I

V

N

N

L

L

L

F

S

G

V

V

K

R

V

A

F

Y

G

G

E

E

L

V

E

E

F

F

W

I

F

L

T

S

L

T

V

V

K

K

I

V

T

V

A

A

I

T

I

F

A

G

M

F

I

I

A

I

G

-

I

L

A

A

I

I

V

I

F

N

G

C

F

G

G

G

V

V

H

P

D

T

T

D

D

H

A

R

G

G

-

Y

-

I

-

G

-

S

-

I

I

I

S

K

H

H

L

K

W

P

S

-

D

-

G

-

F

-

F

F

A

R

T

H

G

G

F

F

G

K

G

G

F

F

V

C

A

S

C

V

F

F

A

T

I

T

V

A

M

A

F

F

A

S

F

F

G

S

G

G

T

T

E

E

I

V

I

I

G

G

I

L

T

A

A

A

G

A

E

E

A

V

E

D

D

N

P

P

A

Q

Q

K

T

A

I

L

R

P

K

H

A

A

V

V

N

K

T

Q

V

V

P

F

V

W

R

R

I

I

I

A

L

I

F

F

Y

Y

I

V

C

V

T

S

L

L

A

I

V

L

I

I

M

G

A

L

I

L

I

I

P

S

W

P

Q

D

T

D

I

P

N

N

-

L

-

M

-

G

-

N

-

G

-

S

S

T

D

S

N

V

S

S

P

P

F

F

V

V

Q

L

I

A

F

I

E

K

N

E

L

A

G

N

L

I

G

K

T

G

A

L

A

P

S

S

I

V

L

F

N

N

I

A

V

V

V

I

I

I

T

I

A

S

A

V

L

V

S

S

A

V

I

T

N

N

S

S

D

S

V

T

F

Y

G

T

A

A

G

G

R

R

M

T

M

L

F

H

G

G

M

M

S

A

H

N

A

L

G

K

Q

Q

A

A

P

P

Q

S

V

F

M

F

K

K

R

Y

V

T

S

D

A

R

N

L

G

G

V

R

P

P

W

L

M

L

T

A

V

-

I

-

M

M

T

I

V

V

A

V

L

L

V

F

G

G

V

-

V

F

L

F

N

A

Y

Y

L

I

-

N

-

E

-

A

-

D

-

K

-

N

-

G

-

N

-

D

I

V

P

S

D

D

Q

T

V

V

F

F

L

N

V

W

I

L

A

L

S

A

L

L

A

S

T

G

F

L

A

S

T

N

I

F

F

F

V

T

W

W

I

G

M

S

I

I

L

C

L

L

S

C

Q

H

-

I

F

F

R

R

S

L

R

A

A

F

Q

K

M

K

S

Q

A

G

D

H

E

S

T

L

A

K

A

E

L

L

K

G

F

F

P

V

V

S

P

P

L

M

W

G

P

I

Y

W

G

G

Q

S

I

C

F

I

A

G

I

L

V

F

F

F

Sites not aligning to the query:

29 modified: Phosphoserine
30 modified: Phosphoserine
37 modified: Phosphoserine

P19145 General amino-acid permease GAP1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
29% identity, 86% coverage: 8:405/463 of query aligns to 83:497/602 of P19145

query
sites
P19145

Q

Q

D

T

G

P

L

L

K

K

R

H

G

H

L

L

T

K

A

N

R

R

H

H

I

L

R

Q

F

M

I

I

A

A

L

I

G

G

S

G

A

A

I

I

G

G

T

T

G

G

L

L

F

L

Y

V

G

G

S

S

A

G

E

T

A

A

I

L

K

R

R

T

A

G

G

G

P

P

-

A

S

S

V

L

L

L

L

I

A

G

Y

W

L

G

I

S

G

T

G

G

I

T

A

M

V

I

Y

Y

L

A

V

M

L

V

R

M

A

A

L

L

G

G

E

E

M

L

A

A

V

V

R

I

N

F

P

P

V

I

S

S

G

G

S

G

F

F

S

T

E

T

Y

Y

A

A

N

T

K

R

H

F

L

I

G

D

P

E

L

S

A

F

G

G

F

Y

M

A

T

N

G

N

W

F

T

N

Y

Y

T

M

F

L

E

Q

M

W

V

L

I

V

V

V

C

L

L

P

A

L

D

E

V

I

T

V

A

S

F

A

G

S

L

I

Y

T

M

V

Q

N

F

F

W

W

F

G

P

T

D

D

V

-

P

P

R

K

W

Y

I

R

W

D

V

G

L

F

A

V

V

A

V

L

F

F

F

W

I

L

G

A

A

I

-

V

-

I

I

I

N

N

L

M

L

F

S

G

V

V

K

K

V

G

F

Y

G

G

E

E

L

A

E

E

F

F

W

V

F

F

T

S

L

F

V

I

K

K

I

V

T

I

A

T

I

V

A

G

M

F

I

I

A

I

G

L

G

G

I

I

A

-

I

-

I

I

V

L

F

N

G

C

F

G

G

G

V

G

H

P

D

T

T

G

D

G

A

Y

G

I

I

G

S

G

H

K

L

Y

W

W

S

H

D

D

G

P

G

G

F

A

F

F

A

A

-

G

-

D

-

T

-

P

-

G

T

A

G

K

F

F

G

K

G

G

F

V

V

C

A

S

C

V

F

F

A

V

I

T

V

A

M

A

F

F

A

S

F

F

G
x
A

G

G

T

S

E

E

I

L

I

V

G

G

I

L

T

A

A

A

G

S

E

E

A

S

E

V

D

E

P

P

A

R

Q

K

T

S

I

V

R

P

K

K

A

A

V

A

N

K

T

Q

V

V

P

F

V

W

R

R

I

I

I

T

L

L

F

F

Y

Y

I

I

C

L

T

S

L

L

A

L

V

M

I

I

M

G

A

L

I

L

I

V

P

P

W

Y

Q

N

-

D

-

K

-

S

-

L

-

I

-

G

-

A

-

S

T

S

I

V

N

D

S

A

D

A

N

A

S

S

P

P

F

F

V

V

Q

I

I

A

F

I

E

K

N

T

L

H

G

G

L

I

G

K

T

G

A

L

A

P

S

S

I

V

L

V

N

N

I

V

V

V

V

I

I

L

T

I

A

A

A

V

L

L

S

S

A

V

I

G

N

N

S

S

D

A

V

I

F

Y

G

A

A

C

G

S

R

R

M

T

M

M

F

V

G

A

M

L

S

A

H

E

A

Q

G

R

Q

F

A

L

P

P

Q

E

V

I

M

F

K

S

R

Y

V

V

S

D

A

R

N

K

G

G

V

R

P

P

W

L

M

V

T

G

V

I

V

A

I

V

M

T

T

S

V

A

A

F

L

G

L

L

V

I

G

A

V

F

V

V

L

A

N

A

Y

S

L

K

I

K

P

E

D

G

Q

E

V

V

F

F

L

N

V

W

I

L

A

L

S

A

L

L

A

S

T

G

F

L

A

S

T

S

I

L

F

F

V

T

W

W

I

G

M

G

I

I

L

C

L

I

S

C

Q

H

F

I

R

R

S

F

R

R

A

K

Q

A

M

L

S

A

A

A

D

Q

-

G

-

R

-

G

-

L

E

D

T

E

A

L

A

S

L

F

K

K

F

S

P

P

V

T

P

G

L

V

W

W

A297 (≠ G217) mutation to V: Impairs basic amino-acids transport and regulation by these amino-acids.

Sites not aligning to the query:

76 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

P48813 High-affinity glutamine permease from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
26% identity, 84% coverage: 2:391/463 of query aligns to 136:538/663 of P48813

query
sites
P48813

N

Q

S

E

D

Q

A

E

A

Q

T

K

Q

Q

D

E

G

N

L

L

K

K

R

K

G

S

L

I

T

K

A

P

R

R

H

H

I

T

R

V

F

M

I

M

A

S

L

L

G

G

S

T

A

G

I

I

G

G

T

T

G

G

L

L

F

L

Y

V

G

G

S

N

A

S

E

K

A

V

I

L

K

N

R

N

A

A

G

G

P

P

S

G

-

G

V

L

L

I

A

G

Y

Y

L

A

I

I

G

M

G

G

I

S

A

C

V

V

Y

Y

L

C

V

I

L

I

R

Q

A

A

L

C

G

G

E

E

M

L

A

A

V

V

-

I

R

Y

N

S

P

D

V

L

S

I

G

G

S

G

F

F

S

N

E

T

Y

Y

A

P

N

L

K

F

H

L

L

V

G

D

P

P

L

A

A

L

G

G

F

F

M

S

T

V

G

A

W

W

T

L

Y

F

T

C

F

L

E

Q

M

W

V

L

I

C

V

V

C

C

L

P

A

L

D

E

V

L

T

V

A

T

F

A

G

S

L

M

Y

T

M

I

Q

K

F

Y

W

W

F

T

P

T

D

S

V

V

P

N

R

P

W

D

I

V

W

F

V

V

L

V

A

I

V

F

V

Y

F

V

F

L

I

I

G

V

A

V

I

I

N

N

L

V

L

F

S

G

V

A

K

K

V

G

F

Y

G

A

E

E

L

A

E

D

F

F

W

F

F

F

T

N

L

C

V

C

K

K

I

I

T

L

A

M

I

I

I

V

A

G

M

F

I

F

A

I

G

L

G

A

I

I

A

I

I

I

I

D

V

C

F

G

G

G

F

A

G

G

V

-

H

-

D

-

T

T

D

D

A

G

G

Y

I

I

-

G

S

S

H

K

L

Y

W

W

S

R

D

D

G

P

G

G

F

A

F

F

A

R

-

G

-

D

-

T

-

P

-

I

T

Q

G

R

F

F

G

K

G

G

F

V

V

V

A

A

C

T

F

F

A

V

I

T

V

A

M

A

F

F

A

A

F

F

G

G

G

M

T

S

E

E

I

Q

I

L

G

A

I

M

T

T

A

A

G

S

E

E

A

Q

E

S

D

N

P

P

A

R

Q

K

T

A

I

I

R

P

K

S

A

A

V

A

N

K

T

K

V

M

P

I

V

Y

R

R

I

I

I

L

L

F

F

V

Y

F

I

L

C

A

T

S

L

L

A

T

V

L

I

V

M

G

A

F

I

L

I

V

P

P

W

Y

Q

T

T

S

I

D

N

Q

-

L

-

G

-

A

-

G

-

S

-

A

S

T

D

K

N

A

S

S

P

P

F

Y

V

V

Q

I

I

A

F

V

E

S

N

S

L

H

G

G

L

V

G

R

T

V

A

V

A

P

S

H

I

F

L

I

N

N

I

A

V

V

V

I

I

L

T

L

A

S

A

V

L

L

S

S

A

V

I

A

N

N

S

G

D

A

V

F

F

Y

G

T

A

S

G

S

R

R

M

I

M

L

F

M

G

S

M

L

S

A

H

K

A

Q

G

G

Q

N

A

A

P

P

Q

K

V

C

M

F

K

D

R

Y

V

I

S

D

A

R

N

E

G

G

V

R

P

P

W

A

M

A

T

A

V

M

V

L

I

V

M

S

T

A

V

L

A

F

L

G

L

V

V

I

G

A

V

F

V

C

L

A

N

S

Y

S

L

K

I

K

P

E

D

E

Q

D

V

V

F

F

L

T

V

W

I

L

A

L

S

A

L

I

A

S

T

G

F

L

A

S

T

Q

I

L

F

F

V

T

W

W

I

I

M

T

I

I

L

C

L

L

S

S

Q

H

F

I

R

R

S

F

R

R

A

R

Q

A

M

M

S

K

Sites not aligning to the query:

132 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Q03770 SPS-sensor component SSY1; Amino-acid permease homolog SSY1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
23% identity, 91% coverage: 3:424/463 of query aligns to 269:771/852 of Q03770

query
sites
Q03770

S

S

D

R

A

A

A

T

T

R

Q

K

D

Y

G

H

L

I

K

Q

R

R

G

K

L

L

T

K

A

V

R

R

H

H

I

I

R

Q

F

M

I

L

A

S

L

I

G

G

S

A

A

C

I

F

G

S

T

V

G

G

L

L

F

F

Y

L

G

T

S

S

A

G

E

K

A

A

I

F

K

S

R

I

A

A

G

G

P

P

-

F

S

G

V

T

L

L

A

G

Y

F

L

G

I

L

G

T

G

G

I

S

A

I

V

I

Y

L

L

A

V

T

L

M

R

L

A

S

L

F

G

T

E

E

M

L

A

S

V

T

R

L

N

I

P

P

V

V

S

S

G

S

S

G

F

F

S

S

E

G

Y

L

A

A

N

S

K

R

H

F

L

V

G

E

P

D

L

A

A

F

G

G

F

F

M

A

T

L

G

G

W

W

T

T

Y

Y

T

W

F

I

E

S

M

C

V

M

I

L

V

A

C

L

L

P

A

A

D

Q

V

V

T

S

A

S

F

S

G
x
T

L

F

Y

Y

M

L

Q

S

F

Y

W

Y

F

N

P

N

-

V

D

N

V

I

P

S

R

K

W

G

I

V

W

T

V

A

L

G

A

F

V

I

V

T

F

L

F

F

I

S

G

A

-

F

-

S

-

I

A

V

I

V

N

N

L

L

S

D

V

V

K

S

V

I

F

M

G

G

E

E

L

I

E

V

F

Y

W

V

F

A

T

G

L

I

V

S

K

K

I

V

T

I

A

I

I

A

I

I

A

L

M

M

I

V

A

F

G

T

G

M

I

I

A

-

I

I

I

L

V

N

F

A

G

G

F

H

G

G

V

-

H

N

D

D

T

I

D

H

A

E

G

G

I

V

S

G

H

F

L

R

W

Y

S

W

D

D

G

S

-

S

-

K

-

S

-

V

-

R

-

N

-

L

-

T

-

Y

G

G

F

L

F

Y

A

R

T

P

G

T

F

F

-

D

-

L

-

A

-

D

-

A

-

G

-

E

-

G

-

S

-

K

-

G

-

I

-

S

-

G

-

P

-

K

G

G

G

R

F

F

V

L

A

A

C

T

F

A

A

S

I

V

V

M

M

L

-

I

-

S

-

T

F

F

A

A

F

F

G

S

G

G

T

V

E

E

I

M

I

T

G

F

I

L

T

A

A

S

G

G

E

E

A

A

E

I

D

N

P

P

A

R

Q

K

T

T

I

I

R

P

K

S

A

A

V

T

N

K

T

R

V

T

P

F

V

S

R

I

I

V

I

L

L

I

F

S

Y

Y

I

V

C

F

T

L

L

I

-

F

-

S

-

V

-

G

-

I

-

N

-

I

-

Y

-

S

-

G

-

D

-

P

-

R

-

L

-

S

-

Y

-

F

-

P

-

G

-

I

-

S

-

E

-

K

-

R

-

Y

-

E

A

A

V

I

I

I

M

K

A

G

I

T

-

G

-

M

-

D

-

W

-

R

-

L

-

R

-

T

-

N

-

C

-

R

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G

I

I

P

D

W

Y

Q

R

T

Q

I

I

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S

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V

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G

-

T

S

G

D

Y

N

S

S

S

P

P

F

W

V

V

Q

V

I

A

F

L

E

Q

N

N

L

F

G

G

L

L

G

C

T

T

A

F

A

A

S

S

I

A

L

F

N

N

I

A

V

I

V

L

I

I

T

F

A

F

A

T

L

A

S

T

A

A

I

G

N

I

S

S

D

S

V

L

F

F

G

S

A

C

G

S

R

R

M

T

M

L

F

Y

G

A

M

M

S

S

H

V

A

Q

G

R

Q

K

A

A

P

P

Q

P

V

V

M

F

K

E

R

I

V

C

S

S

A

K

N

R

G

G

V

V

P

P

W

Y

M

V

T

S

V

V

V

I

I

F

M

S

T

S

V

L

A

F

L

-

V

-

G

-

V

S

V

V

L

I

N

A

Y

Y

L

I

I

A

P

V

D

D

Q

Q

V

T

-

A

-

I

-

E

-

N

F

F

L

D

V

V

I

L

A

A

S

N

L

V

A

S

T

S

F

A

A

S

T

T

I

S

F

I

V

I

W

W

I

M

M

G

I

L

L

N

L

L

S

S

Q

F

F

L

R

R

-

F

-

Y

-

A

-

L

-

K

S

Q

R

R

A

K

Q

D

M

I

S

I

A

S

D

R

E

N

T

D

A

S

L

Y

K

P

F

Y

P

K

V

S

P

P

L

F

W

Q

P

P

Y

Y

G

L

Q

A

I

I

F

Y

A

G

I

L

V

V

F

G

L

C

A

S

F

L

V

F

I

V

V

I

L

F

L

M

G

G

T382 (≠ G115) mutation T->H,L: Constitutively active, up-regulates amino acid permease transcription in response to subthreshold concentrations of amino acids.; mutation to K: In SSY1-102; constitutively active, up-regulates amino acid permease transcription in the absence of amino-acids.; mutation to R: Constitutively active, up-regulates amino acid permease transcription in the absence of amino acids.

P76037 Putrescine importer PuuP from Escherichia coli (strain K12) (see paper)
28% identity, 41% coverage: 51:240/463 of query aligns to 58:242/461 of P76037

query
sites
P76037

A

S

Y

Y

L

L

I

L

G

A

G

L

I

A

A

G

V

V

Y

L

L

F

V

T

L

A

R

I

A

S

L

Y

G

G

E

K

M

L

A

V

V

R

R

Q

N

F

P

P

V

E

S

A

G

G

S

S

F

A

S

Y

E

T

Y

Y

A

A

N

Q

K

K

H

S

L

I

G

N

P

P

L

H

A

V

G

G

F

F

M

M

T

V

G

G

W

W

T

S

Y

S

T

L

F

L

E

D

M
x
Y

V

L

I

F

V

L

C

P

L

M

A

I

D

N

V

V

T

L

A

L

F

A

G

K

L

I

Y

Y

M

L

Q

S

F

A

W

L

F

F

P

P

D

E

V

V

P

P

R

P

W

W

I

V

W

W

V

V

L

V

A

T

V

F

V

V

F

A

F

I

I

L

G

T

A

A

I

A

N

N

L

L

L

K

S

S

V

V

K

N

V

L

F

V

G

A

E

N

L

F

E

N

F

T

W

L

F

F

T

V

L

L

V

V

K

Q

I

I

T

S

A

I

I

M

I

V

A

V

M

F

I

I

A

F

G

L

G

V

I

V

A

Q

I

-

V

-

F

-

G

-

F

-

G

G

V

L

H

H

D

K

T

G

D

E

A

-

G

G

I

V

S

G

H

T

L

V

W

W

S

S

D

L

G

Q

G

P

F

F

F

I

A

S

T

E

G

N

-

A

-

H

F

L

G

I

G

P

F

I

V

I

A

T

C

G

F

A

A

T

I

I

V

V

M

C

F

F

A

S

F

F

G

L

G

G

T

F

E

D

I

A

I

V

G

T

I

T

T

L

A

S

G

E

E

E

A

T

E

P

D

D

P

A

A

A

Q

R

T

V

I

I

R

P

K

K

A

A

V

I

Y110 (≠ M103) mutation to X: The uptake activity is reduced to one-eighth of that of wild-type.

O34739 Serine/threonine exchanger SteT from Bacillus subtilis (strain 168) (see paper)
23% identity, 89% coverage: 7:420/463 of query aligns to 3:404/438 of O34739

query
sites
O34739

T

T

Q

E

D

D

-

N

G

G

L

L

K

K

R

K

G

E

L

I

T

G

A

L

R

L

H

F

I

A

R

L

F

T

I

L

A

V

L

I

G

G

S

T

A

I

I

I

G

G

T

S

G

G

L

V

F

F

Y

M

G

K

S

P

A

G

E

A

A

V

I

L

K

A

R

Y

A

S

G

G

P

D

S

S

-

K

-

M

V

A

L

L

L

F

A

A

Y

W

L

L