SitesBLAST

K81 (≠ A129) mutation to R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, C-202, V-332 and V-342.
S202 (= S258) mutation to C: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, V-332 and V-342.
I332 (= I387) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-342.
M342 (≠ V397) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-332.

Sites not aligning to the query:

51 G→S: Shows 4.3- and 107-fold increase of affinity to glyphosate and glycine, respectively. Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with R-54, R-81, C-202, V-332 and V-342.
54 A→R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-81, C-202, V-332 and V-342.

7cyxA Crystal strcuture of glycine oxidase from bacillus cereus atcc 14579 (see paper)
23% identity, 96% coverage: 10:410/417 of query aligns to 4:353/363 of 7cyxA

query
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binding flavin-adenine dinucleotide: I7 (≠ V13), G8 (= G14), G10 (= G16), V11 (= V17), I12 (≠ V18), V30 (= V36), E31 (≠ D37), K32 (≠ R38), E38 (≠ D44), A39 (= A45), S40 (= S46), A43 (≠ N49), G45 (= G51), L46 (≠ W52), V171 (vs. gap), G200 (≠ S258), G201 (= G259), W203 (≠ R261), G298 (= G356), R300 (= R358), P301 (= P359), Y326 (≠ G383), R327 (≠ M384), N328 (≠ W385), G329 (= G386), I330 (= I387)

6j39A Crystal structure of cmis2 with inhibitor (see paper)
26% identity, 95% coverage: 18:413/417 of query aligns to 11:363/368 of 6j39A

query
sites
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binding (3R)-3-[(carboxymethyl)sulfanyl]nonanoic acid: M44 (≠ L75), P46 (= P77), N49 (≠ P80), R243 (≠ P294), Y252 (≠ A303), Y267 (≠ M317), R308 (= R358), R334 (≠ M384), I335 (≠ W385)
binding flavin-adenine dinucleotide: I11 (≠ V18), V29 (= V36), D30 (= D37), P31 (= P62), E32 (= E63), K36 (≠ L67), A37 (≠ Q68), S38 (≠ Y69), V40 (≠ L71), S41 (≠ R72), A42 (= A73), G43 (≠ M74), M44 (≠ L75), A174 (≠ D228), A203 (≠ S258), W206 (≠ R261), I228 (≠ S282), Y252 (≠ A303), R308 (= R358), S333 (≠ G383), R334 (≠ M384), I335 (≠ W385), G336 (= G386), V337 (≠ I387), Q338 (≠ A388)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 7, 9, 10

6j38A Crystal structure of cmis2 (see paper)
26% identity, 95% coverage: 18:413/417 of query aligns to 11:363/368 of 6j38A

query
sites
6j38A

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A

G
|
G

S

L

R
|
R

P

P

C

G

T

S

T

P

D

D

G

G

L

L

P

P

L

V

I

L

G

G

A

W

T

T

R

A

S

V

P

P

R

N

V

L

H

L

V

V

A

A

G

T

G

G

H

H

G
x
S

M
x
R

W
x
I

G
|
G

I
x
V

A
x
Q

L

L

G

A

P

P

L

I

T

T

G

A

K

D

M

V

V

M

A

G

D

E

S

M

M

L

T

V

G

T

A

G

Q

R

T

T

P

P

T

E

V

V

M

A

S

K

H

A

F

F

binding flavin-adenine dinucleotide: I11 (≠ V18), V29 (= V36), D30 (= D37), P31 (= P62), K36 (≠ L67), A37 (≠ Q68), S38 (≠ Y69), S41 (≠ R72), A42 (= A73), G43 (≠ M74), M44 (≠ L75), A174 (≠ D228), A203 (≠ S258), W206 (≠ R261), G226 (= G280), G306 (= G356), R308 (= R358), S333 (≠ G383), R334 (≠ M384), I335 (≠ W385), G336 (= G386), V337 (≠ I387), Q338 (≠ A388)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 7, 9

O31616 Glycine oxidase; GO; EC 1.4.3.19 from Bacillus subtilis (strain 168) (see 3 papers)
23% identity, 53% coverage: 184:406/417 of query aligns to 129:351/369 of O31616

query
sites
O31616

E

E

P

P

S

Y

L

A

G

S

Q

G

G

D

V

I

K

F

A

G

G

A

L

S

R

F

L

I

R

Q

N

D

Q

D

R

V

F

H

I

V

D

E

P

P

P

Y

L

F

F

V

V

C

N

K

S

A

L

Y

A

V

D

K

A

A

V

A

R

K

E

M

R

L

G

G

G

A

E

E

I

I

V

F

S

E

G

H

F

T

D

P

V
|
V

T

L

Q

H

I

V

D

E

D

R

R

D

V

G

G

E

G

A

V

L

T

F

V

I

R

K

S

T

A

P

H

S

G

G

D

D

S

V

R

W

D

-

G

A

D

N

A

H

V

V

I

V

S

A

S

S

G

G

A

V

R

W

L

S

N

G

K

M

L

F

A

F

A

K

P

Q

F

L

G

G

V

L

R

N

K

N

L

A

V

F

Q

L

A

P

G

V

R

K

G

G

Y

E

S

C

F

L

S

S

V

V

K

W

P

N

E

D

H

D

L

I

P

P

K

L

N

T

P

K

V

T

Y

L

F

Y

P

H

T

D

Q
x
H

R

C

V

Y

A

I

C

V

T

P

P

R

L

K

H

S

D

G

R

R

F

L

R

V

V

V

A

G

G

A

M

T

M

M

E

K

F

P

R

G

S

D

P

W

D

S

A

E

P

T

L

P

D

D

P

L

R

G

V

L

Q

E

A

S

I

V

I

M

D

K

A

K

A

A

K

K

P

T

M

M

L

L

S

P

G

A

I

I

D

Q

W

N

T

M

A

K

R

V

E

D

E

R

E

F

W

W

V

A

G

G

S

L

R
|
R

P

P

C

G

T

T

T

K

D

D

G

G

L

K

P

P

L

Y

I

I

G

G

A

-

T

-

R

R

S

H

P

P

-

E

-

D

-

S

R

R

V

I

H

L

V

F

A

A

G

A

G

G

H
|
H

G
x
F

M
x
R

W
x
N

G
|
G

I
|
I

A
x
L

L

L

G

A

P

P

L

A

T

T

G

G

K

A

M

L

V

I

A

S

D

D

S

L

M

I

T

M

G

N

A

K

Q

E

T

V

V174 (= V229) binding FAD
H244 (≠ Q300) mutation to A: 2-fold decrease in catalytic efficiency on glycine and similar catalytic efficiency on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and R-54.
R302 (= R358) binding substrate
327:333 (vs. 382:388, 43% identical) binding FAD
R329 (≠ M384) binding substrate

Sites not aligning to the query:

14:15 binding FAD
34:35 binding FAD
42:43 binding FAD
47:49 binding FAD
51 G→R: 130-fold decrease in catalytic efficiency on glycine and 28-fold increase in that on glyphosate.; G→S: 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with R-54 and A-244.
54 A→R: 20-fold decrease in catalytic efficiency on glycine and 34-fold increase in that on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and A-244.

3if9A Crystal structure of glycine oxidase g51s/a54r/h244a mutant in complex with inhibitor glycolate (see paper)
23% identity, 53% coverage: 184:406/417 of query aligns to 129:351/364 of 3if9A

query
sites
3if9A

E

E

P

P

S

Y

L

A

G

S

Q

G

G

D

V

I

K

F

A

G

G

A

L

S

R

F

L

I

R

Q

N

D

Q

D

R

V

F

H

I

V

D

E

P

P

P

Y

L

F

F

V

V

C

N

K

S

A

L

Y

A

V

D

K

A

A

V

A

R

K

E

M

R

L

G

G

G

A

E

E

I

I

V

F

S

E

G

H

F

T

D
x
P

V
|
V

T

L

Q

H

I

V

D

E

D

R

R

D

V

G

G

E

G

A

V

L

T

F

V

I

R

K

S

T

A

P

H

S

G

G

D

D

S

V

R

W

D

-

G

A

D

N

A

H

V

V

I

V

S

A

S
|
S

G
|
G

A

V

R
x
W

L

S

N

G

K

M

L
x
F

A

F

A

K

P

Q

F

L

G

G

V

L

R

N

K

N

L

A

V

F

Q

L

A

P

G

V

R

K

G

G

Y

E

S

C

F

L

S

S

V

V

K

W

P

N

E

D

H

D

L

I

P

P

K

L

N

T

P

K

V

T

Y

L

F

Y

P

H

T

D

Q

A

R

C

V
x
Y

A

I

C

V

T

P

P

R

L

K

H

S

D

G

R

R

F

L

R

V

V

V

A

G

G

A

M

T

M

M

E

K

F

P

R

G

S

D

P

W

D

S

A

E

P

T

L

P

D

D

P

L

R

G

V

L

Q

E

A

S

I

V

I

M

D

K

A

K

A

A

K

K

P

T

M

M

L

L

S

P

G

A

I

I

D

Q

W

N

T

M

A

K

R

V

E

D

E

R

E

F

W

W

V

A

G
|
G

S

L

R
|
R

P

P

C

G

T

T

T

K

D

D

G

G

L

K

P

P

L

Y

I

I

G

G

A

-

T

-

R

R

S

H

P

P

-

E

-

D

-

S

R

R

V

I

H

L

V

F

A

A

G

A

G

G

H
|
H

G
x
F

M
x
R

W
x
N

G
|
G

I
|
I

A

L

L

L

G

A

P

P

L

A

T

T

G

G

K

A

M

L

V

I

A

S

D

D

S

L

M

I

T

M

G

N

A

K

Q

E

T

V

binding flavin-adenine dinucleotide: P173 (≠ D228), V174 (= V229), S202 (= S258), G203 (= G259), W205 (≠ R261), F209 (≠ L265), G300 (= G356), R302 (= R358), H327 (= H382), F328 (≠ G383), R329 (≠ M384), N330 (≠ W385), G331 (= G386), I332 (= I387)
binding glycolic acid: Y246 (≠ V302), R302 (= R358), R329 (≠ M384)

Sites not aligning to the query:

active site: 47, 48, 49
binding flavin-adenine dinucleotide: 11, 13, 15, 34, 35, 42, 43, 46, 47, 48, 49

1ng3A Complex of thio (glycine oxidase) with acetyl-glycine (see paper)
23% identity, 53% coverage: 184:406/417 of query aligns to 129:351/364 of 1ng3A

query
sites
1ng3A

E

E

P

P

S

Y

L

A

G

S

Q

G

G

D

V

I

K

F

A

G

G

A

L

S

R

F

L

I

R

Q

N

D

Q

D

R

V

F

H

I

V

D

E

P

P

P

Y

L

F

F

V

V

C

N

K

S

A

L

Y

A

V

D

K

A

A

V

A

R

K

E

M

R

L

G

G

G

A

E

E

I

I

V

F

S

E

G

H

F

T

D

P

V
|
V

T

L

Q

H

I

V

D

E

D

R

R

D

V

G

G

E

G

A

V

L

T

F

V

I

R

K

S

T

A

P

H

S

G

G

D

D

S

V

R

W

D

-

G

A

D

N

A

H

V

V

I

V

S

A

S
|
S

G
|
G

A

V

R
x
W

L

S

N

G

K

M

L
x
F

A

F

A

K

P

Q

F

L

G

G

V

L

R

N

K

N

L

A

V

F

Q

L

A

P

G

V

R

K

G

G

Y

E

S

C

F

L

S

S

V

V

K

W

P

N

E

D

H

D

L

I

P

P

K

L

N

T

P

K

V

T

Y

L

F

Y

P

H

T

D

Q

H

R

C

V
x
Y

A

I

C

V

T

P

P

R

L

K

H

S

D

G

R
|
R

F

L

R

V

V

V

A

G

G

A

M

T

M

M

E

K

F

P

R

G

S

D

P

W

D

S

A

E

P

T

L

P

D

D

P

L

R

G

V

L

Q

E

A

S

I

V

I

M

D

K

A

K

A

A

K

K

P

T

M

M

L

L

S

P

G

P

I

I

D

Q

W

N

T

M

A

K

R

V

E

D

E

R

E

F

W

W

V

A

G
|
G

S

L

R
|
R

P

P

C

G

T

T

T

K

D

D

G

G

L

K

P

P

L

Y

I

I

G

G

A

-

T

-

R

R

S

H

P

P

-

E

-

D

-

S

R

R

V

I

H

L

V

F

A

A

G

A

G

G

H
|
H

G

F

M
x
R

W
x
N

G
|
G

I
|
I

A

L

L

L

G

A

P

P

L

A

T

T

G

G

K

A

M

L

V

I

A

S

D

D

S

L

M

I

T

M

G

N

A

K

Q

E

T

V

binding acetylamino-acetic acid: Y246 (≠ V302), R302 (= R358), R329 (≠ M384)
binding flavin-adenine dinucleotide: V174 (= V229), S202 (= S258), G203 (= G259), W205 (≠ R261), F209 (≠ L265), G300 (= G356), R302 (= R358), H327 (= H382), R329 (≠ M384), N330 (≠ W385), G331 (= G386), I332 (= I387)
binding phosphate ion: R254 (= R310)

Sites not aligning to the query:

active site: 47, 48, 49
binding flavin-adenine dinucleotide: 11, 13, 15, 33, 34, 35, 41, 42, 43, 46, 47, 48, 49
binding phosphate ion: 89

4yshA Crystal structure of glycine oxidase from geobacillus kaustophilus
29% identity, 41% coverage: 246:417/417 of query aligns to 194:367/370 of 4yshA

query
sites
4yshA

G

G

D

G

S

T

R

F

D

A

G

A

D

E

A

A

V

V

I

I

S

A

S
|
S

G
|
G

A

A

R
x
W

L

A

N

A

K

R

L

L

A

G

A

A

P

R

F

V

G

G

V

L

R

S

K

L

L

S

V

V

Q

Y

A

P

G

V

R

K

G

G

Y

E

S

C

F

V

S

M

V

V

K

R

-

A

P

P

E

V

H

P

L

L

P

L

K

Q

N

T

P

T

V

V

Y

F

F

A

P

K

T

N

Q

G

R

C

V

Y

A

I

C

V

T

P

P

K

L

S

H

G

D

N

R

R

F

L

R

L

V
x
I

A

G

G

A

M

T

M

S

E

T

F

P

R

G

S

T

P

F

D

D

A

R

P

R

L

V

D

S

P

A

R

G

V

V

Q

M

A

N

I
x
L

I

L

D

H

A

R

A

A

K

A

P

H

M

L

L

V

S

P

G

D

I

I

D

E

W

Q

T

A

A

E

R

W

E

V

E

A

E

S

W

W

V

S

G
|
G

S

I

R
|
R

P

P

C

Q

T

T

T

E

D

D

G

G

L

L

P

P

L

Y

I

L

G

G

A

E

T

H

R

P

S

E

P

R

R

R

-

G

V

L

H

F

V

V

A

A

G

A

G

G

H
|
H

G

Y

M
x
R

W
x
N

G
|
G

I
|
I

A
x
L

L

L

G

S

P

P

L

L

T

T

G

G

K

L

M

L

V

V

A

A

D

D

S

L

M

V

T

E

G

R

A

K

Q

E

T

T

P

A

T

F

V

D

M

L

S

A

H

P

F

F

D

S

P

L

L

T

R

R

active site: I262 (≠ V313), L283 (≠ I334), G305 (= G356), N335 (≠ W385), L338 (≠ A388)
binding flavin-adenine dinucleotide: S206 (= S258), G207 (= G259), W209 (≠ R261), R307 (= R358), H332 (= H382), R334 (≠ M384), N335 (≠ W385), G336 (= G386), I337 (= I387)

Sites not aligning to the query:

active site: 45, 48, 49, 52
binding flavin-adenine dinucleotide: 10, 12, 14, 32, 33, 34, 40, 41, 42, 45, 46, 47, 48, 178

4yshB Crystal structure of glycine oxidase from geobacillus kaustophilus
29% identity, 41% coverage: 246:417/417 of query aligns to 194:367/368 of 4yshB

query
sites
4yshB

G

G

D

G

S

T

R

F

D

A

G

A

D

E

A

A

V

V

I

I

S

A

S
|
S

G

G

A

A

R
x
W

L

A

N

A

K

R

L

L

A

G

A

A

P

R

F

V

G

G

V

L

R

S

K

L

L

S

V

V

Q

Y

A

P

G

V

R

K

G

G

Y

E

S

C

F

V

S

M

V

V

K

R

-

A

P

P

E

V

H

P

L

L

P

L

K

Q

N

T

P

T

V

V

Y

F

F

A

P

K

T

N

Q
x
G

R

C

V
x
Y

A

I

C

V

T

P

P

K

L

S

H

G

D

N

R

R

F

L

R

L

V
x
I

A

G

G
x
A

M

T

M

S

E

T

F

P

R

G

S

T

P

F

D

D

A

R

P

R

L

V

D

S

P

A

R

G

V

V

Q

M

A

N

I
x
L

I

L

D

H

A

R

A

A

K

A

P

H

M

L

L

V

S

P

G

D

I

I

D

E

W

Q

T

A

A

E

R

W

E

V

E

A

E

S

W

W

V

S

G
|
G

S

I

R
|
R

P

P

C

Q

T

T

T

E

D

D

G

G

L

L

P

P

L

Y

I

L

G

G

A

E

T

H

R

P

S

E

P

R

R

R

-

G

V

L

H

F

V

V

A

A

G

A

G

G

H
|
H

G

Y

M
x
R

W
x
N

G
|
G

I
|
I

A
x
L

L

L

G

S

P

P

L

L

T

T

G

G

K

L

M

L

V

V

A

A

D

D

S

L

M

V

T

E

G

R

A

K

Q

E

T

T

P

A

T

F

V

D

M

L

S

A

H

P

F

F

D

S

P

L

L

T

R

R

active site: I262 (≠ V313), L283 (≠ I334), G305 (= G356), N335 (≠ W385), L338 (≠ A388)
binding flavin-adenine dinucleotide: S206 (= S258), W209 (≠ R261), R307 (= R358), H332 (= H382), R334 (≠ M384), N335 (≠ W385), G336 (= G386), I337 (= I387), L338 (≠ A388)
binding glycine: G249 (≠ Q300), Y251 (≠ V302), Y251 (≠ V302), A264 (≠ G315), R307 (= R358), R334 (≠ M384), R334 (≠ M384)

Sites not aligning to the query:

active site: 45, 48, 49, 52
binding flavin-adenine dinucleotide: 9, 10, 12, 14, 32, 33, 34, 40, 41, 42, 45, 46, 48, 178

Q5L2C2 Glycine oxidase; GO; GOX; GOXK; EC 1.4.3.19 from Geobacillus kaustophilus (strain HTA426)
29% identity, 41% coverage: 246:417/417 of query aligns to 196:369/377 of Q5L2C2

query
sites
Q5L2C2

G

G

D

G

S

T

R

F

D

A

G

A

D

E

A

A

V

V

I

I

S

A

S

S

G

G

A

A

R

W

L

A

N

A

K

R

L

L

A

G

A

A

P

R

F

V

G

G

V

L

R

S

K

L

L

S

V

V

Q

Y

A

P

G

V

R

K

G

G

Y

E

S

C

F

V

S

M

V

V

K

R

-

A

P

P

E

V

H

P

L

L

P

L

K

Q

N

T

P

T

V

V

Y

F

F

A

P

K

T

N

Q

G

R

C

V

Y

A

I

C

V

T

P

P

K

L

S

H

G

D

N

R

R

F

L

R

L

V

I

A

G

G

A

M

T

M

S

E

T

F

P

R

G

S

T

P

F

D

D

A

R

P

R

L

V

D

S

P

A

R

G

V

V

Q

M

A

N

I

L

D

H

A

R

A

A

K

A

P

H

M

L

L

V

S

P

G

D

I

I

D

E

W

Q

T

A

A

E

R

W

E

V

E

A

E

S

W

W

V

S

G

G

S

I

R
|
R

P

P

C

Q

T

T

T

E

D

D

G

G

L

L

P

P

L

Y

I

L

G

G

A

E

T

H

R

P

S

E

P

R

R

R

-

G

V

L

H

F

V

V

A

A

G

A

G

G

H
|
H

G
x
Y

M
x
R

W
x
N

G
|
G

I
|
I

A
x
L

L

L

G

S

P

P

L

L

T

T

G

G

K

L

M

L

V

V

A

A

D

D

S

L

M

V

T

E

G

R

A

K

Q

E

T

T

P

A

T

F

V

D

M

L

S

A

H

P

F

F

D

S

P

L

L

T

R

R

R309 (= R358) binding substrate
334:340 (vs. 382:388, 43% identical) binding FAD
R336 (≠ M384) binding substrate

Sites not aligning to the query:

14:15 binding FAD
34:35 binding FAD
42:43 binding FAD
47:49 binding FAD
180 binding FAD

8griF Orf1-e312a-glycine-glycylthricin (see paper)
24% identity, 53% coverage: 195:417/417 of query aligns to 157:392/482 of 8griF

query
sites
8griF

L

L

R

H

L

I

R

E

N

G

Q

E

R

G

F

S

I

V

D

D

P

S

P

G

L

I

F

L

V

L

N

A

S

A

L

L

A

E

D

R

A

S

V

F

R

L

E

Q

R

A

G

G

E

R

I

L

-

H

-

P

V

V

S
x
D

G
x
A

F

T

D

E

V

I

T

R

Q

A

I

S

D

H

D

G

R

R

V

V

G

E

G

G

V

V

T

V

V

T

R

D

S

D

A

-

H

-

G

G

D

D

S

F

R

L

D

P

G

A

D

G

A

H

V

V

I

V

S

A

S
x
A

G
|
G

A

A

R
|
R

L

S

N

Q

K

R

L

L

A

V

A

A

P

A

F

L

-

P

-

G

-

L

-

A

-

H

-

R

-

I

-

P

-

R

-

I

-

Y

-

D

-

G

-

V

G

G

V

V

R

S

K

A

L

L

V

V

Q

D

A

T

G

W

R

D

G

G

Y

S

S

G

F

-

S

-

V

-

K

-

P

-

E

-

H

-

L

-

P

P

K

A

N

T

P

V

V

L

Y

R

F

T

P

S

T

N

Q

R

R

A

V

F

A

A

C
|
C

T

G

P

-

L

L

H
|
H

D

L

R

V

F

P

R

R

V

A

A

G

G

G

M

S

M

V

E

Y

F

I

R

G

S

A

P

T

D
x
N

A

A

-

V

P

C

L

L

D

E

P

P

R

R

R

G

V

A

Q

A

A
x
S

I

I

I

A

D

E

-

T

-

V

-

F

-

L

-

F

-

N

-

C

A

A

A

T

K

H

P

Q

M

L

L

H

S

R

G

G

I

L

D

N

W

G

T

S

A

E

R

L

E

R

E

K

E

V

W

Q

V

V

G
|
G

S

S

R
|
R

P

P

C

A

T

P

T

I

D

D

G

G

L

F

P

P

L

L

I

I

G

G

A

G

T

T

R

S

S

V

P

E

R

G

V

L

H

W

V

M

A

L

G

S

G

G

H
x
T

G
x
Y

M
x
R

W
x
D

G
|
G

I
x
L

A
x
H

L

M

G

S

P

P

L

L

T

L

G

A

K

R

M

H

V

V

A

V

D

S

S

L

M

M

T

D

G

G

A

G

Q

T

T

G

P

V

T

D

V

G

M

L

S

R

H

E

F

F

D

R

P

P

L

E

R

R

binding flavin-adenine dinucleotide: D189 (≠ S225), A190 (≠ G226), A220 (≠ S258), G221 (= G259), R223 (= R261), G331 (= G356), R333 (= R358), T357 (≠ H382), Y358 (≠ G383), R359 (≠ M384), D360 (≠ W385), G361 (= G386), L362 (≠ I387), H363 (≠ A388)
binding glycine: H275 (= H308), R333 (= R358), R359 (≠ M384)
binding [(2~{R},3~{R},4~{S},5~{R},6~{R})-6-[(~{E})-[(3~{a}~{S},7~{R},7~{a}~{S})-7-oxidanyl-4-oxidanylidene-3,3~{a},5,6,7,7~{a}-hexahydro-1~{H}-imidazo[4,5-c]pyridin-2-ylidene]amino]-5-(2-azanylethanoylamino)-2-(hydroxymethyl)-4-oxidanyl-oxan-3-yl] carbamate: C272 (= C304), N290 (≠ D323), S301 (≠ A333)

Sites not aligning to the query:

7xxmA Orf1-glycine-4-aminobutylthricin complex (see paper)
24% identity, 53% coverage: 195:417/417 of query aligns to 157:392/482 of 7xxmA

query
sites
7xxmA

L

L

R

H

L

I

R

E

N

G

Q

E

R

G

F

S

I

V

D

D

P

S

P

G

L

I

F

L

V

L

N

A

S

A

L

L

A

E

D

R

A

S

V

F

R

L

E

Q

R

A

G

G

E

R

I

L

-

H

-

P

V

V

S
x
D

G
x
A

F

T

D

E

V

I

T

R

Q

A

I

S

D

H

D

G

R

R

V

V

G

E

G

G

V

V

T

V

V

T

R

D

S

D

A

-

H

-

G

G

D

D

S

F

R

L

D

P

G

A

D

G

A

H

V

V

I

V

S

A

S
x
A

G
|
G

A

A

R
|
R

L

S

N

Q

K

R

L

L

A

V

A

A

P

A

F

L

-

P

-

G

-

L

-

A

-

H

-

R

-

I

-

P

-

R

-

I

-

Y

-

D

-

G

-

V

G
|
G

V

V

R

S

K

A

L

L

V

V

Q

D

A

T

G

W

R

D

G

G

Y

S

S

G

F

-

S

-

V

-

K

-

P

-

E

-

H

-

L

-

P

P

K

A

N

T

P

V

V

L

Y

R

F

T

P

S

T

N

Q

R

R

A

V

F

A

A

C

C

T

G

P

-

L

L

H

H

D

L

R

V

F

P

R

R

V

A

A

G

G

G

M

S

M

V

E

Y

F

I

R

G

S

A

P

T

D
x
N

A

A

-

V

P

C

L

L

D

E

P

P

R
|
R

R

G

V

A

Q

A

A
x
S

I

I

I
x
E

D

E

-

T

-

V

-

F

-

L

-

F

-

N

-

C

A

A

A

T

K

H

P

Q

M

L

L

H

S

R

G

G

I

L

D

N

W

G

T

S

A

E

R

L

E

R

E

K

E

V

W

Q

V

V

G
|
G

S

S

R
|
R

P

P

C

A

T

P

T

I

D

D

G

G

L

F

P

P

L

L

I

I

G

G

A

G

T

T

R

S

S

V

P

E

R

G

V

L

H

W

V

M

A

L

G

S

G

G

H
x
T

G
x
Y

M
x
R

W
x
D

G
|
G

I
x
L

A
x
H

L

M

G

S

P

P

L

L

T

L

G

A

K

R

M

H

V

V

A

V

D

S

S

L

M

M

T

D

G

G

A

G

Q

T

T

G

P

V

T

D

V

G

M

L

S

R

H

E

F

F

D

R

P

P

L

E

R

R

binding flavin-adenine dinucleotide: D189 (≠ S225), A190 (≠ G226), A220 (≠ S258), G221 (= G259), R223 (= R261), G246 (= G270), G331 (= G356), R333 (= R358), T357 (≠ H382), Y358 (≠ G383), R359 (≠ M384), D360 (≠ W385), G361 (= G386), L362 (≠ I387), H363 (≠ A388)
binding glycine: R333 (= R358), R359 (≠ M384)
binding [(2~{R},3~{R},4~{S},5~{R},6~{R})-6-[(~{E})-[(3~{a}~{S},7~{R},7~{a}~{S})-7-oxidanyl-4-oxidanylidene-3,3~{a},5,6,7,7~{a}-hexahydro-1~{H}-imidazo[4,5-c]pyridin-2-ylidene]amino]-5-(4-azanylbutanoylamino)-2-(hydroxymethyl)-4-oxidanyl-oxan-3-yl] carbamate: N290 (≠ D323), R297 (= R329), S301 (≠ A333), E303 (≠ I335)

Sites not aligning to the query:

7xxpA F316a-glycine-streptothricin f complex (see paper)
24% identity, 53% coverage: 195:417/417 of query aligns to 157:392/482 of 7xxpA

query
sites
7xxpA

L

L

R

H

L

I

R

E

N

G

Q

E

R

G

F

S

I

V

D

D

P

S

P

G

L

I

F

L

V

L

N

A

S

A

L

L

A

E

D

R

A

S

V

F

R

L

E

Q

R

A

G

G

E

R

I

L

-

H

-

P

V

V

S
x
D

G
x
A

F

T

D

E

V

I

T

R

Q

A

I

S

D

H

D

G

R

R

V

V

G

E

G

G

V

V

T

V

V

T

R

D

S

D

A

-

H

-

G

G

D

D

S

F

R

L

D

P

G

A

D

G

A

H

V

V

I

V

S

A

S
x
A

G
|
G

A

A

R
|
R

L

S

N

Q

K

R

L

L

A

V

A

A

P

A

F

L

-

P

-

G

-

L

-

A

-

H

-

R

-

I

-

P

-

R

-

I

-

Y

-

D

-

G

-

V

G

G

V

V

R

S

K

A

L

L

V

V

Q

D

A

T

G

W

R

D

G

G

Y

S

S

G

F

-

S

-

V

-

K

-

P

-

E

-

H

-

L

-

P

P

K

A

N

T

P

V

V

L

Y

R

F

T

P

S

T

N

Q

R

R

A

V

F

A

A

C

C

T

G

P

-

L

L

H
|
H

D

L

R

V

F

P

R

R

V

A

A

G

G

G

M

S

M

V

E

Y

F

I

R

G

S

A

P

T

D
x
N

A

A

-

V

P

C

L

L

D

E

P

P

R
|
R

R

G

V

A

Q

A

A
x
S

I

I

I
x
E

D

E

-

T

-

V

-

A

-

L

-

F

-

N

-

C

A

A

A

T

K

H

P

Q

M

L

L

H

S

R

G

G

I

L

D

N

W

G

T

S

A

E

R

L

E

R

E

K

E

V

W

Q

V

V

G
|
G

S

S

R
|
R

P
|
P

C

A

T

P

T

I

D

D

G

G

L

F

P

P

L

L

I

I

G

G

A

G

T

T

R

S

S

V

P

E

R

G

V

L

H

W

V

M

A

L

G

S

G

G

H
x
T

G
x
Y

M
x
R

W
x
D

G
|
G

I
x
L

A
x
H

L

M

G

S

P

P

L

L

T

L

G

A

K

R

M

H

V

V

A

V

D

S

S

L

M

M

T

D

G

G

A

G

Q

T

T

G

P

V

T

D

V

G

M

L

S

R

H

E

F

F

D

R

P

P

L

E

R

R

binding flavin-adenine dinucleotide: D189 (≠ S225), A190 (≠ G226), A220 (≠ S258), G221 (= G259), R223 (= R261), G331 (= G356), R333 (= R358), P334 (= P359), T357 (≠ H382), Y358 (≠ G383), R359 (≠ M384), D360 (≠ W385), G361 (= G386), L362 (≠ I387), H363 (≠ A388)
binding glycine: H275 (= H308), R333 (= R358), R359 (≠ M384)
binding Streptothricin F: N290 (≠ D323), R297 (= R329), S301 (≠ A333), E303 (≠ I335)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 7, 8, 9, 10, 11, 12, 33, 37, 38, 41, 42, 45, 47, 48
binding Streptothricin F: 414, 457, 461

Query Sequence

>WP_021333986.1 NCBI__GCF_002893965.1:WP_021333986.1
MGTRQSPDRVVVVGAGVVGLSTAWFLQERGVEVTVVDRDGVAADASWGNAGWLAPALTLP
LPEPAVLQYGLRAMLSPSSPVYVPLTTDLKLIRFLVGFARHCTPSKWQEAMSVYTEVNRT
ALGAYDELADGGVKELTRLADPFLAAFASEKDRQTLVDEFHHVEAAGGEVDFDLLDYDAI
HSLEPSLGQGVKAGLRLRNQRFIDPPLFVNSLADAVRERGGEIVSGFDVTQIDDRVGGVT
VRSAHGDSRDGDAVVISSGARLNKLAAPFGVRKLVQAGRGYSFSVKPEHLPKNPVYFPTQ
RVACTPLHDRFRVAGMMEFRSPDAPLDPRRVQAIIDAAKPMLSGIDWTAREEEWVGSRPC
TTDGLPLIGATRSPRVHVAGGHGMWGIALGPLTGKMVADSMTGAQTPTVMSHFDPLR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory