SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_022046005.1 NCBI__GCF_001406815.1:WP_022046005.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 2 hits to proteins with known functional sites (download)

12asA Asparagine synthetase mutant c51a, c315a complexed with l-asparagine and amp (see paper)
45% identity, 90% coverage: 20:321/336 of query aligns to 6:316/327 of 12asA

query
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active site: D43 (= D57), R97 (= R111), Q113 (= Q126), D232 (= D237), E245 (= E250), S248 (= S253)
binding adenosine monophosphate: R97 (= R111), L106 (≠ I119), S108 (= S121), V111 (= V124), Q113 (= Q126), L246 (= L251), S247 (= S252), G293 (= G298), R296 (= R301)
binding asparagine: D43 (= D57), S69 (= S83), A71 (= A85), K74 (= K88), D115 (= D128), Y215 (= Y229), R252 (= R257), G291 (= G296)

11asA Asparagine synthetase mutant c51a, c315a complexed with l-asparagine (see paper)
45% identity, 90% coverage: 20:321/336 of query aligns to 6:316/327 of 11asA

query
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11asA

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active site: D43 (= D57), R97 (= R111), Q113 (= Q126), D232 (= D237), E245 (= E250), S248 (= S253)
binding asparagine: D43 (= D57), A71 (= A85), Q113 (= Q126), Y215 (= Y229), S248 (= S253), R252 (= R257)

Query Sequence

>WP_022046005.1 NCBI__GCF_001406815.1:WP_022046005.1
MENLILPKQYKSELNLHDTQLAIKTVKDFFQALLAQRLHLTRVSAPLFVDPATGLNDNLN
GYERPVTFGIKEQDDKNAEVVHSLAKWKRYALKKYGFQVGEGIYTDMNAIRRDESTDNIH
SIFVDQWDWEKIIHKEDRNLDYLKETVRTVYKCLRKTEQYMAIQYDYIDLILPKEIFFIT
SQELEDLFPDNTPKEREYYITKAKGAVCIMQIGDTLESGEPHDGRAPDYDDWALNADILV
YYPVLDIALELSSMGIRVDKTALISQLDKAGCPERKDLPFQKSIIDGTLPYTIGGGIGQS
RICMFFLRKAHIGEVQSSLWPEETIAACEEHGIQLL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory