SitesBLAST

C26 (≠ N11) mutation to A: Lower ATPase activity and transport efficiency.
F27 (≠ Y12) mutation to L: Lower ATPase activity and transport efficiency.
F45 (≠ L30) mutation to L: Lower ATPase activity and transport efficiency.
C54 (≠ S39) mutation to T: Loss of ATPase activity and transport.
L60 (= L45) mutation to F: Lower ATPase activity and transport efficiency.
L76 (≠ I61) mutation to P: Lower ATPase activity and transport efficiency.
V135 (≠ T120) mutation to M: Loss of ATPase activity and transport.
D172 (= D157) mutation to N: Loss of ATPase activity and transport.
C276 (vs. gap) mutation to A: Lower ATPase activity and transport efficiency.
E297 (= E262) mutation E->K,D: Lower ATPase activity and transport efficiency.; mutation to Q: Loss of ATPase activity and transport.

8y5iA Cryo-em structure of e.Coli spermidine transporter potd-potabc in translocation intermidiate state (see paper)
41% identity, 89% coverage: 3:314/352 of query aligns to 3:335/358 of 8y5iA

query
sites
8y5iA

V

V

E

Q

M

L

R

A

D

G

I

I

C

R

K

K

N

C

Y
x
F

G

D

N

G

F
x
K

R

E

A

V

S

I

D

P

H

Q

V

L

S

D

F

L

G

T

I

I

E

N

K

N

G

G

K

E

L

F

V

L

A

T

L

L

G

G

P

P

S

S

G
|
G

S

C

G

G

K
|
K

T
|
T

L

V

L

L

R

R

M

L

I

I

A

A

G

G

L

L

E

E

T

T

P

V

N

D

S

S

G

G

D

R

I

I

Y

M

I

L

D

D

G

N

Q

E

R

D

V

I

N

T

D

H

V

V

P

P

A

A

A

E

K

N

R

R

G

Y

I

V

G

N

F

T

V

V

F

F

Q

Q

N

S

Y

Y

A

A

L

L

F

F

R

P

Y

H

M

M

T

T

V

V

Y

F

D

E

N

N

V

V

A

A

F

F

G

G

L

L

E

R

L

M

A

Q

K

K

V

T

P

P

K

A

E

E

I

I

K

T

K

P

R

R

V

V

T

M

E

E

L

A

L

L

E

R

L

M

T

V

G

Q

L

L

S

E

G

T

M

F

E

A

K

Q

R
|
R

Y

K

P

P

N

H

Q
|
Q

L

L

S
|
S

G

G

Q

Q

R

Q

Q

Q

R

R

V

V

A

A

F

I

A

A

R

R

A

A

L

V

A

V

P

N

N

K

P

P

Q

R

V

L

L

L

D

D

E

Q

P

S

F

L

A

S

A

A

I

L

D

D

A

Y

K

K

V

L

R

R

T

K

E

Q

L

M

R

Q

N

N

W

E

L

L

R

K

E

A

M

L

V

Q

V

R

K

K

L

L

G

G

I

I

T

T

S

F

I

V

F

F

V

V

T

T

H

H

D

D

Q

Q

D

E

E

E

A

A

V

L

E

T

V

M

A

S

D

D

E

R

I

I

I

V

T

M

N

R

H

D

G

G

H

R

I

I

E

E

Q

Q

M

D

G

G

T

T

P

P

M

R

E

E

I

I

Y

Y

K

E

S

E

P

P

D

K

T

N

P

L

F

F

V

V

A

A

Q

G

F

F

I

I

G

G

-

E

-

I

-

N

-

M

-

F

R

N

S

A

S

T

I

V

V

I

E

E

H

R

Y

L

E

D

R

E

L

Q

L

R

G

V

F

R

D

A

P

N

V

V

E

E

G

G

-

R

-

E

-

C

-

N

-

I

-

Y

-

V

-

N

-

F

-

A

-

V

-

E

-

P

-

G

-

Q

A

K

T

L

H

H

A

V

V

L

I

L

R

R

P

P

E

E

F

D

V

L

R

R

M

V

S

E

R

E

S

I

E

N

P

D

Q

N

H

H

M

A

S

E

A

G

A

L

E

-

R

I

G

G

I

Y

V

V

K

R

D

E

V

R

I

N

F

Y

R

K

G

G

N

M

H

T

L

L

D

E

V

S

V

V

D

E

V

L

-

E

-

N

G

G

G

K

I

M

P

V

I

M

V

V

T

S

E

E

R

F

S

F

L

N

E

E

K

D

D

D

P

P

binding adenosine-5'-triphosphate: F12 (≠ Y12), K15 (≠ F15), G38 (= G38), K41 (= K41), T42 (= T42), T43 (= T43), R128 (= R128), Q132 (= Q132), S134 (= S134)

1g291 Malk (see paper)
50% identity, 66% coverage: 3:234/352 of query aligns to 4:241/372 of 1g291

query
sites
1g291

V

V

E

R

M

L

R

V

D

D

I

V

C

W

K

K

N

V

Y

F

G

G

N

E

F

V

R

T

A

A

S

V

D

R

H

E

V

M

S

S

F

L

G

E

I

V

E

K

K

D

G

G

K

E

L

F

V

M

A

I

L

L

G

G

P

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

T
|
T

L

T

L

L

R

R

M

M

I

I

A

A

G

G

L

L

E

E

T

E

P

P

N

S

S

R

G

G

D

Q

I

I

Y

Y

I

I

D

G

G

D

Q

K

R

L

V

V

N

A

D
|
D

-

P

-
x
E

-
x
K

-

G

-

I

-

F

V

V

P

P

A

P

A
x
K

K
x
D

R

R

G

D

I

I

G

A

F

M

V

V

F

F

Q

Q

N

S

Y

Y

A

A

L

L

F

Y

R

P

Y

H

M

M

T

T

V

V

Y

Y

D

D

N

N

V

I

A

A

F

F

G

P

L

L

E

K

L

L

A

R

K

K

V

V

P

P

K

R

K

Q

E

E

I

I

K

D

K

Q

R

R

V

V

T

R

E

E

L

V

L

A

E

E

L

L

T

L

G

G

L

L

S

T

G

E

M

L

E

L

K

N

R

R

Y

K

P

P

N

R

Q

E

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

F

L

A

G

R

R

A

A

L

I

A

V

P

R

N

K

P

P

Q

Q

V

V

L

F

L

L

L

M

D

D

E

E

P

P

F

L

A

S

A

N

I

L

D

D

A

A

K

K

V

L

R

R

T

V

E

R

L

M

R

R

N

A

W

E

L

L

R

K

E

K

M

L

V

Q

V

R

K

Q

L

L

G

G

I

V

T

T

S

T

I

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

D

V

E

E

A

A

V

M

E

T

V

M

A

G

D

D

E

R

I

I

I

A

I

V

T

M

N

N

H

R

G

G

H

V

I

L

E

Q

Q

Q

M

V

G

G

T

S

P

P

M

D

E

E

I

V

Y

Y

K

D

S

K

P

P

D

A

T

N

P

T

F

F

V

V

A

A

Q

G

F

F

I

I

G

G

binding magnesium ion: D69 (= D68), E71 (vs. gap), K72 (vs. gap), K79 (≠ A72), D80 (≠ K73)
binding pyrophosphate 2-: S38 (= S37), G39 (= G38), C40 (≠ S39), G41 (= G40), K42 (= K41), T43 (= T42), T44 (= T43)

Sites not aligning to the query:

binding magnesium ion: 292, 293, 359

P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
46% identity, 66% coverage: 3:234/352 of query aligns to 4:235/369 of P19566

query
sites
P19566

V

V

E

Q

M

L

R

R

D

N

I

V

C

T

K

K

N

A

Y

W

G

G

N

D

F

V

R

V

A

V

S

S

D

K

H

D

V

I

S

N

F

L

G

D

I

I

E

H

K

D

G

G

K

E

L

F

V

V

A

V

L

F

L

V

G

G

P

P

S

S

G

G

S

C

G

G

K

K

T

S

T

T

L

L

R

R

M

M

I

I

A

A

G

G

L

L

E

E

T

T

P

I

N

T

S

S

G

G

D

D

I

L

Y

F

I

I

D

G

G

E

Q

T

R

R

V

M

N

N

D

D

V

I

P

P

A

P

A

A

K

E

R

R

G

G

I

V

G

G

F

M

V

V

F

F

Q

Q

N

S

Y

Y

A

A

L
|
L

F

Y

R

P

Y

H

M

L

T

S

V

V

Y

A

D

E

N

N

V

M

A

S

F

F

G

G

L

L

E

K

L

L

A

A

K

G

V

A

P

K

K

K

K

E

E

V

I

M

K

N

K

Q

R

R

V

V

T

N

E

Q

L

V

L

A

E

E

L

V

T

L

G

Q

L

L

S

A

G

H

M

L

E

L

K

E

R

R

Y

K

P

P

N

K

Q

A

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

F

I

A

G

R

R

A

T

L

L

A

V

P

A

N

E

P

P

Q

R

V

V

L

F

L

L

D

D

E

E

P
|
P

F

L

A

S

A

N

I

L

D
|
D

A

A

K

A

V

L

R

R

T

V

E

Q

L

M

R

R

N

I

W

E

L

I

R

S

E

R

M

L

V

H

V

K

K

R

L

L

G

G

I

R

T

T

S

M

I

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

D

V

E

E

A

A

V

M

E

T

V

L

A

A

D

D

E

K

I

I

I

V

T

L

N

D

H

A

G

G

H

R

I

V

E

A

Q

Q

M

V

G

G

T

K

P

P

M

L

E

E

I

L

Y

Y

K

H

S

Y

P

P

D

A

T

D

P

R

F

F

V

V

A

A

Q

G

F

F

I

I

G

G

L86 (= L85) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
P160 (= P159) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
D165 (= D164) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.

Sites not aligning to the query:

306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
46% identity, 66% coverage: 3:234/352 of query aligns to 4:235/371 of P68187

query
sites
P68187

V

V

E

Q

M

L

R

Q

D

N

I

V

C

T

K

K

N

A

Y

W

G

G

N

E

F

V

R

V

A

V

S

S

D

K

H

D

V

I

S

N

F

L

G

D

I

I

E

H

K

E

G

G

K

E

L

F

V

V

A

V

L

F

L

V

G

G

P

P

S

S

G

G

S

C

G

G

K

K

T

S

T

T

L

L

R

R

M

M

I

I

A

A

G

G

L

L

E

E

T

T

P

I

N

T

S

S

G

G

D

D

I

L

Y

F

I

I

D

G

G

E

Q

K

R

R

V

M

N

N

D

D

V

T

P

P

A

P

A

A

K

E

R

R

G

G

I

V

G

G

F

M

V

V

F

F

Q

Q

N

S

Y

Y

A
|
A

L

L

F

Y

R

P

Y

H

M

L

T

S

V

V

Y

A

D

E

N

N

V

M

A

S

F

F

G

G

L

L

E

K

L

L

A

A

K

G

V

A

P
x
K

K

K

K

E

E

V

I

I

K

N

K

Q

R

R

V
|
V

T

N

E

Q

L
x
V

L

A

E
|
E

L

V

T

L

G

Q

L

L

S
x
A

G

H

M

L

E

L

K

D

R

R

Y

K

P

P

N

K

Q

A

L

L

S

S

G

G

G
|
G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

F

I

A

G

R

R

A

T

L

L

A

V

P

A

N

E

P

P

Q

S

V

V

L

F

L

L

D
|
D

E

E

P

P

F

L

A

S

A

N

I

L

D

D

A

A

K

A

V

L

R

R

T

V

E

Q

L

M

R

R

N

I

W

E

L

I

R

S

E

R

M

L

V

H

V

K

K

R

L

L

G

G

I

R

T

T

S

M

I

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

D

V

E

E

A

A

V

M

E

T

V

L

A

A

D

D

E

K

I

I

I

V

T

L

N

D

H

A

G

G

H

R

I

V

E

A

Q

Q

M

V

G

G

T

K

P

P

M

L

E

E

I

L

Y

Y

K

H

S

Y

P

P

D

A

T

D

P
x
R

F

F

V

V

A

A

Q

G

F

F

I

I

G

G

A85 (= A84) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (≠ P105) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (= V113) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (≠ L116) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (= E118) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (≠ S123) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G136) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D157) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
R228 (≠ P227) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.

Sites not aligning to the query:

241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
267 W→G: Normal maltose transport but constitutive mal gene expression.
278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
46% identity, 66% coverage: 3:234/352 of query aligns to 3:234/374 of 2awnB

query
sites
2awnB

V

V

E

Q

M

L

R

Q

D

N

I

V

C

T

K

K

N

A

Y
x
W

G

G

N

E

F

V

R

V

A
x
V

S

S

D

K

H

D

V

I

S

N

F

L

G

D

I

I

E

H

K

E

G

G

K

E

L

F

V

V

A

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

S

C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

M

M

I

I

A

A

G

G

L

L

E

E

T

T

P

I

N

T

S

S

G

G

D

D

I

L

Y

F

I

I

D

G

G

E

Q

K

R

R

V

M

N

N

D

D

V

T

P

P

A

P

A

A

K

E

R

R

G

G

I

V

G

G

F

M

V

V

F

F

Q

Q

N

S

Y

Y

A

A

L

L

F

Y

R

P

Y

H

M

L

T

S

V

V

Y

A

D

E

N

N

V

M

A

S

F

F

G

G

L

L

E

K

L

L

A

A

K

G

V

A

P

K

K

K

K

E

E

V

I

I

K

N

K

Q

R

R

V

V

T

N

E

Q

L

V

L

A

E

E

L

V

T

L

G

Q

L

L

S

A

G

H

M

L

E

L

K

D

R

R

Y

K

P

P

N

K

Q

A

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

F

I

A

G

R

R

A

T

L

L

A

V

P

A

N

E

P

P

Q

S

V

V

L

F

L

L

D

D

E

E

P

P

F

L

A

S

A

N

I

L

D

D

A

A

K

A

V

L

R

R

T

V

E

Q

L

M

R

R

N

I

W

E

L

I

R

S

E

R

M

L

V

H

V

K

K

R

L

L

G

G

I

R

T

T

S

M

I

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

D

V

E

E

A

A

V

M

E

T

V

L

A

A

D

D

E

K

I

I

I

V

T

L

N

D

H

A

G

G

H

R

I

V

E

A

Q

Q

M

V

G

G

T

K

P

P

M

L

E

E

I

L

Y

Y

K

H

S

Y

P

P

D

A

T

D

P

R

F

F

V

V

A

A

Q

G

F

F

I

I

G

G

binding adenosine-5'-diphosphate: W12 (≠ Y12), V17 (≠ A17), S37 (= S37), G38 (= G38), G40 (= G40), K41 (= K41), S42 (≠ T42), T43 (= T43)

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
46% identity, 66% coverage: 3:234/352 of query aligns to 3:234/371 of 3puyA

query
sites
3puyA

V

V

E

Q

M

L

R

Q

D

N

I

V

C

T

K

K

N

A

Y
x
W

G

G

N

E

F

V

R

V

A

V

S

S

D

K

H

D

V

I

S

N

F

L

G

D

I

I

E

H

K

E

G

G

K

E

L

F

V

V

A

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

M

M

I

I

A

A

G

G

L

L

E

E

T

T

P

I

N

T

S

S

G

G

D

D

I

L

Y

F

I

I

D

G

G

E

Q

K

R

R

V

M

N

N

D

D

V

T

P

P

A

P

A

A

K

E

R

R

G

G

I

V

G

G

F

M

V

V

F

F

Q
|
Q

N

S

Y

Y

A

A

L

L

F

Y

R

P

Y

H

M

L

T

S

V

V

Y

A

D

E

N

N

V

M

A

S

F

F

G

G

L

L

E

K

L

L

A

A

K

G

V

A

P

K

K

K

K

E

E

V

I

I

K

N

K

Q

R

R

V

V

T

N

E

Q

L

V

L

A

E

E

L

V

T

L

G

Q

L

L

S

A

G

H

M

L

E

L

K

D

R
|
R

Y

K

P

P

N

K

Q
x
A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

R

R

Q

Q

R

R

V

V

A

A

F

I

A

G

R

R

A

T

L

L

A

V

P

A

N

E

P

P

Q

S

V

V

L

F

L

L

D

D

E
|
E

P

P

F

L

A

S

A

N

I

L

D

D

A

A

K

A

V

L

R

R

T

V

E

Q

L

M

R

R

N

I

W

E

L

I

R

S

E

R

M

L

V

H

V

K

K

R

L

L

G

G

I

R

T

T

S

M

I

I

F

Y

V

V

T

T

H
|
H

D

D

Q

Q

D

V

E

E

A

A

V

M

E

T

V

L

A

A

D

D

E

K

I

I

I

V

T

L

N

D

H

A

G

G

H

R

I

V

E

A

Q

Q

M

V

G

G

T

K

P

P

M

L

E

E

I

L

Y

Y

K

H

S

Y

P

P

D

A

T

D

P

R

F

F

V

V

A

A

Q

G

F

F

I

I

G

G

binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ Y12), S37 (= S37), G38 (= G38), C39 (≠ S39), G40 (= G40), K41 (= K41), S42 (≠ T42), T43 (= T43), Q81 (= Q81), R128 (= R128), A132 (≠ Q132), S134 (= S134), G136 (= G136), Q137 (= Q137), E158 (= E158), H191 (= H191)
binding magnesium ion: S42 (≠ T42), Q81 (= Q81)

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
46% identity, 66% coverage: 3:234/352 of query aligns to 3:234/371 of 3puxA

query
sites
3puxA

V

V

E

Q

M

L

R

Q

D

N

I

V

C

T

K

K

N

A

Y
x
W

G

G

N

E

F

V

R

V

A

V

S

S

D

K

H

D

V

I

S

N

F

L

G

D

I

I

E

H

K

E

G

G

K

E

L

F

V

V

A

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

M

M

I

I

A

A

G

G

L

L

E

E

T

T

P

I

N

T

S

S

G

G

D

D

I

L

Y

F

I

I

D

G

G

E

Q

K

R

R

V

M

N

N

D

D

V

T

P

P

A

P

A

A

K

E

R

R

G

G

I

V

G

G

F

M

V

V

F

F

Q
|
Q

N

S

Y

Y

A

A

L

L

F

Y

R

P

Y

H

M

L

T

S

V

V

Y

A

D

E

N

N

V

M

A

S

F

F

G

G

L

L

E

K

L

L

A

A

K

G

V

A

P

K

K

K

K

E

E

V

I

I

K

N

K

Q

R

R

V

V

T

N

E

Q

L

V

L

A

E

E

L

V

T

L

G

Q

L

L

S

A

G

H

M

L

E

L

K

D

R
|
R

Y

K

P

P

N

K

Q

A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

R

R

Q

Q

R

R

V

V

A

A

F

I

A

G

R

R

A

T

L

L

A

V

P

A

N

E

P

P

Q

S

V

V

L

F

L

L

D

D

E

E

P

P

F

L

A

S

A

N

I

L

D

D

A

A

K

A

V

L

R

R

T

V

E

Q

L

M

R

R

N

I

W

E

L

I

R

S

E

R

M

L

V

H

V

K

K

R

L

L

G

G

I

R

T

T

S

M

I

I

F

Y

V

V

T

T

H
|
H

D

D

Q

Q

D

V

E

E

A

A

V

M

E

T

V

L

A

A

D

D

E

K

I

I

I

V

T

L

N

D

H

A

G

G

H

R

I

V

E

A

Q

Q

M

V

G

G

T

K

P

P

M

L

E

E

I

L

Y

Y

K

H

S

Y

P

P

D

A

T

D

P

R

F

F

V

V

A

A

Q

G

F

F

I

I

G

G

binding adenosine-5'-diphosphate: W12 (≠ Y12), G38 (= G38), C39 (≠ S39), G40 (= G40), K41 (= K41), S42 (≠ T42), T43 (= T43), R128 (= R128), S134 (= S134), Q137 (= Q137)
binding beryllium trifluoride ion: S37 (= S37), G38 (= G38), K41 (= K41), Q81 (= Q81), S134 (= S134), G136 (= G136), H191 (= H191)
binding magnesium ion: S42 (≠ T42), Q81 (= Q81)

3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
46% identity, 66% coverage: 3:234/352 of query aligns to 3:234/371 of 3puwA

query
sites
3puwA

V

V

E

Q

M

L

R

Q

D

N

I

V

C

T

K

K

N

A

Y
x
W

G

G

N

E

F

V

R

V

A
x
V

S

S

D

K

H

D

V

I

S

N

F

L

G

D

I

I

E

H

K

E

G

G

K

E

L

F

V

V

A

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

M

M

I

I

A

A

G

G

L

L

E

E

T

T

P

I

N

T

S

S

G

G

D

D

I

L

Y

F

I

I

D

G

G

E

Q

K

R

R

V

M

N

N

D

D

V

T

P

P

A

P

A

A

K

E

R

R

G

G

I

V

G

G

F

M

V

V

F

F

Q
|
Q

N

S

Y

Y

A

A

L

L

F

Y

R

P

Y

H

M

L

T

S

V

V

Y

A

D

E

N

N

V

M

A

S

F

F

G

G

L

L

E

K

L

L

A

A

K

G

V

A

P

K

K

K

K

E

E

V

I

I

K

N

K

Q

R

R

V

V

T

N

E

Q

L

V

L

A

E

E

L

V

T

L

G

Q

L

L

S

A

G

H

M

L

E

L

K

D

R
|
R

Y

K

P

P

N

K

Q
x
A

L

L

S
|
S

G
|
G

Q
|
Q

R

R

Q

Q

R

R

V

V

A

A

F

I

A

G

R

R

A

T

L

L

A

V

P

A

N

E

P

P

Q

S

V

V

L

F

L

L

D

D

E
|
E

P

P

F

L

A

S

A

N

I

L

D

D

A

A

K

A

V

L

R

R

T

V

E

Q

L

M

R

R

N

I

W

E

L

I

R

S

E

R

M

L

V

H

V

K

K

R

L

L

G

G

I

R

T

T

S

M

I

I

F

Y

V

V

T

T

H
|
H

D

D

Q

Q

D

V

E

E

A

A

V

M

E

T

V

L

A

A

D

D

E

K

I

I

I

V

T

L

N

D

H

A

G

G

H

R

I

V

E

A

Q

Q

M

V

G

G

T

K

P

P

M

L

E

E

I

L

Y

Y

K

H

S

Y

P

P

D

A

T

D

P

R

F

F

V

V

A

A

Q

G

F

F

I

I

G

G

binding adenosine-5'-diphosphate: W12 (≠ Y12), V17 (≠ A17), G38 (= G38), C39 (≠ S39), G40 (= G40), K41 (= K41), S42 (≠ T42), T43 (= T43), R128 (= R128), A132 (≠ Q132), S134 (= S134), Q137 (= Q137)
binding tetrafluoroaluminate ion: S37 (= S37), G38 (= G38), K41 (= K41), Q81 (= Q81), S134 (= S134), G135 (= G135), G136 (= G136), E158 (= E158), H191 (= H191)
binding magnesium ion: S42 (≠ T42), Q81 (= Q81)

3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
46% identity, 66% coverage: 3:234/352 of query aligns to 3:234/371 of 3puvA

query
sites
3puvA

V

V

E

Q

M

L

R

Q

D

N

I

V

C

T

K

K

N

A

Y
x
W

G

G

N

E

F

V

R

V

A
x
V

S

S

D

K

H

D

V

I

S

N

F

L

G

D

I

I

E

H

K

E

G

G

K

E

L

F

V

V

A

V

L

F

L

V

G

G

P

P

S

S

G
|
G

S
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

M

M

I

I

A

A

G

G

L

L

E

E

T

T

P

I

N

T

S

S

G

G

D

D

I

L

Y

F

I

I

D

G

G

E

Q

K

R

R

V

M

N

N

D

D

V

T

P

P

A

P

A

A

K

E

R

R

G

G

I

V

G

G

F

M

V

V

F

F

Q
|
Q

N

S

Y

Y

A

A

L

L

F

Y

R

P

Y

H

M

L

T

S

V

V

Y

A

D

E

N

N

V

M

A

S

F

F

G

G

L

L

E

K

L

L

A

A

K

G

V

A

P

K

K

K

K

E

E

V

I

I

K

N

K

Q

R

R

V

V

T

N

E

Q

L

V

L

A

E

E

L

V

T

L

G

Q

L

L

S

A

G

H

M

L

E

L

K

D

R
|
R

Y

K

P

P

N

K

Q
x
A

L

L

S
|
S

G

G

Q
|
Q

R

R

Q

Q

R

R

V

V

A

A

F

I

A

G

R

R

A

T

L

L

A

V

P

A

N

E

P

P

Q

S

V

V

L

F

L

L

D

D

E

E

P

P

F

L

A

S

A

N

I

L

D

D

A

A

K

A

V

L

R

R

T

V

E

Q

L

M

R

R

N

I

W

E

L

I

R

S

E

R

M

L

V

H

V

K

K

R

L

L

G

G

I

R

T

T

S

M

I

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

D

V

E

E

A

A

V

M

E

T

V

L

A

A

D

D

E

K

I

I

I

V

T

L

N

D

H

A

G

G

H

R

I

V

E

A

Q

Q

M

V

G

G

T

K

P

P

M

L

E

E

I

L

Y

Y

K

H

S

Y

P

P

D

A

T

D

P

R

F

F

V

V

A

A

Q

G

F

F

I

I

G

G

binding adenosine-5'-diphosphate: W12 (≠ Y12), V17 (≠ A17), G38 (= G38), C39 (≠ S39), G40 (= G40), K41 (= K41), S42 (≠ T42), T43 (= T43), R128 (= R128), A132 (≠ Q132), S134 (= S134), Q137 (= Q137)
binding magnesium ion: S42 (≠ T42), Q81 (= Q81)

1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
46% identity, 66% coverage: 3:234/352 of query aligns to 1:232/367 of 1q12A

query
sites
1q12A

V

V

E

Q

M

L

R

Q

D

N

I

V

C

T

K

K

N

A

Y
x
W

G

G

N

E

F

V

R

V

A

V

S

S

D

K

H

D

V

I

S

N

F

L

G

D

I

I

E

H

K

E

G

G

K

E

L

F

V

V

A

V

L

F

L

V

G

G

P

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

L

R

R

M

M

I

I

A

A

G

G

L

L

E

E

T

T

P

I

N

T

S

S

G

G

D

D

I

L

Y

F

I

I

D

G

G

E

Q

K

R

R

V

M

N

N

D

D

V

T

P

P

A

P

A

A

K

E

R

R

G

G

I

V

G

G

F

M

V

V

F

F

Q

Q

N

S

Y

Y

A

A

L

L

F

Y

R

P

Y

H

M

L

T

S

V

V

Y

A

D

E

N

N

V

M

A

S

F

F

G

G

L

L

E

K

L

L

A

A

K

G

V

A

P

K

K

K

K

E

E

V

I

I

K

N

K

Q

R

R

V

V

T

N

E

Q

L

V

L

A

E

E

L

V

T

L

G

Q

L

L

S

A

G

H

M

L

E

L

K

D

R
|
R

Y

K

P

P

N

K

Q
x
A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

R

R

Q

Q

R

R

V

V

A

A

F

I

A

G

R

R

A

T

L

L

A

V

P

A

N

E

P

P

Q

S

V

V

L

F

L

L

D

D

E

E

P

P

F

L

A

S

A

N

I

L

D

D

A

A

K

A

V

L

R

R

T

V

E

Q

L

M

R

R

N

I

W

E

L

I

R

S

E

R

M

L

V

H

V

K

K

R

L

L

G

G

I

R

T

T

S

M

I

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

D

V

E

E

A

A

V

M

E

T

V

L

A

A

D

D

E

K

I

I

I

V

T

L

N

D

H

A

G

G

H

R

I

V

E

A

Q

Q

M

V

G

G

T

K

P

P

M

L

E

E

I

L

Y

Y

K

H

S

Y

P

P

D

A

T

D

P

R

F

F

V

V

A

A

Q

G

F

F

I

I

G

G

binding adenosine-5'-triphosphate: W10 (≠ Y12), S35 (= S37), G36 (= G38), C37 (≠ S39), G38 (= G40), K39 (= K41), S40 (≠ T42), T41 (= T43), R126 (= R128), A130 (≠ Q132), S132 (= S134), G134 (= G136), Q135 (= Q137)

1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
48% identity, 66% coverage: 3:234/352 of query aligns to 7:230/353 of 1vciA

query
sites
1vciA

V

V

E

K

M

L

R

E

D

N

I

L

C

T

K

K

N

R

Y
x
F

G

G

N

N

F
|
F

R

T

A

A

S

V

D

N

H

K

V

L

S

N

F

L

G

T

I

I

E

K

K

D

G

G

K

E

L

F

V

L

A

V

L

L

G

G

P

P

S
|
S

G
|
G

S

C

G
|
G

K
|
K

T
|
T

L

T

L

L

R

R

M

M

I

I

A

A

G

G

L

L

E

E

T

E

P

P

N

T

S

E

G

G

D

R

I

I

Y

Y

I

F

D

G

G

D

Q

R

R

D

V

V

N

T

D

Y

V

L

P

P

A

P

A

K

K

D

R

R

G

N

I

I

G

S

F

M

V

V

F

F

Q

Q

N

-

Y

-

A

-

L

-

F

-

R

-

Y

H

M

M

T

T

V

V

Y

Y

D

E

N

N

V

I

A

A

F

F

G

P

L

L

E

K

L

-

A

-

K

K

V

F

P

P

K

K

K

D

E

E

I

I

K

D

K

K

R

R

V

V

T

R

E

W

L

A

E

E

L

L

T

L

G

Q

L

I

S

E

G

E

M

L

E

L

K

N

R

R

Y

Y

P

P

N

A

Q

Q

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

F

V

A

A

R

R

A

A

L

I

A

V

P

V

N

E

P

P

Q

D

V

V

L

L

L

M

D

D

E

E

P

P

F

L

A

S

A

N

I

L

D

D

A

A

K

K

V

L

R

R

T

V

E

A

L

M

R

R

N

A

W

E

L

I

R

K

E

K

M

L

V

Q

K

K

L

L

G

K

I

V

T

T

S

T

I

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

D

V

E

E

A

A

V

M

E

T

V

M

A

G

D

D

E

R

I

I

I

A

I

V

T

M

N

N

H

R

G

G

H

Q

I

L

E

L

Q

Q

M

I

G

G

T

S

P

P

M

T

E

E

I

V

Y

Y

K

L

S

R

P

P

D

N

T

S

P

V

F

F

V

V

A

A

Q

T

F

F

I

I

G

G

binding adenosine-5'-triphosphate: F16 (≠ Y12), F19 (= F15), S41 (= S37), G42 (= G38), G44 (= G40), K45 (= K41), T46 (= T42), T47 (= T43)

8hprD Lpqy-sugabc in state 4 (see paper)
46% identity, 66% coverage: 8:238/352 of query aligns to 8:239/362 of 8hprD

query
sites
8hprD

I

V

C

T

K

K

N

S

Y
|
Y

G

P

N

D

F

V

R

R

A

A

S

A

-

V

D

K

H

E

V

F

S

S

F

M

G

T

I

I

E

A

K

D

G

G

K

E

L

F

V

I

A

I

L

L

L

V

G

G

P

P

S
|
S

G

G

S
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

T

L

L

R

N

M

M

I

I

A

A

G

G

L

L

E

E

T

E

P

I

N

T

S

S

G

G

D

E

I

L

Y

R

I

I

D

G

G

G

Q

E

R

R

V

V

N

N

D

E

V

K

P

A

A

P

A

K

K

D

R

R

G

D

I

I

G

A

F

M

V

V

F

F

Q
|
Q

N

S

Y

Y

A

A

L

L

F

Y

R

P

Y

H

M

M

T

T

V

V

Y

R

D

Q

N

N

V

I

A

A

F

F

G

P

L

L

E

T

L

L

A

A

K

K

V

V

P

P

K

K

K

A

E

E

I

I

K

A

R

K

V

V

T

E

E

E

L

T

L

A

E

K

L

I

T

L

G

D

L

L

S

S

G

E

M

L

E

L

K

D

R
|
R

Y

K

P

P

N

G

Q
|
Q

L

L

S
|
S

G
|
G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

F

M

A

G

R

R

A

A

L

I

A

V

P

R

N

S

P

P

Q

K

V

A

L

F

L

L

L

M

D

D

E
x
Q

P

P

F

L

A

S

A

N

I

L

D

D

A

A

K

K

V

L

R

R

T

V

E

Q

L

M

R

R

N

A

W

E

L

I

R

S

E

R

M

L

V

Q

V

D

K

R

L

L

G

G

I

T

T

T

S

T

I

V

F

Y

V

V

T

T

H
|
H

D

D

Q

Q

D

T

E

E

A

A

V

M

E

T

V

L

A

G

D

D

E

R

I

V

T

M

N

L

H

A

G

G

H

E

I

V

E

Q

Q

Q

M

I

G

G

T

T

P

P

M

D

E

E

I

L

Y

Y

K

S

S

S

P

P

D

A

T

N

P

L

F

F

V

V

A

A

Q

G

F

F

I

I

G

G

R

S

S

P

S

A

I

M

binding adenosine-5'-triphosphate: Y12 (= Y12), S38 (= S37), C40 (≠ S39), G41 (= G40), K42 (= K41), S43 (≠ T42), T44 (= T43), Q82 (= Q81), R129 (= R128), Q133 (= Q132), S135 (= S134), G136 (= G135), G137 (= G136), Q159 (≠ E158), H192 (= H191)
binding magnesium ion: S43 (≠ T42), Q82 (= Q81)

8hprC Lpqy-sugabc in state 4 (see paper)
46% identity, 66% coverage: 8:238/352 of query aligns to 8:239/363 of 8hprC

query
sites
8hprC

I

V

C

T

K

K

N

S

Y
|
Y

G

P

N

D

F

V

R

R

A

A

S

A

-

V

D

K

H

E

V

F

S

S

F

M

G

T

I

I

E

A

K

D

G

G

K

E

L

F

V

I

A

I

L

L

L

V

G

G

P

P

S
|
S

G
|
G

S

C

G
|
G

K
|
K

T
x
S

T

T

L

T

L

L

R

N

M

M

I

I

A

A

G

G

L

L

E

E

T

E

P

I

N

T

S

S

G

G

D

E

I

L

Y

R

I

I

D

G

G

G

Q

E

R

R

V

V

N

N

D

E

V

K

P

A

A

P

A

K

K

D

R

R

G

D

I

I

G

A

F

M

V

V

F

F

Q
|
Q

N

S

Y

Y

A

A

L

L

F

Y

R

P

Y

H

M

M

T

T

V

V

Y

R

D

Q

N

N

V

I

A

A

F

F

G

P

L

L

E

T

L

L

A

A

K

K

V

V

P

P

K

K

K

A

E

E

I

I

K

A

R

K

V

V

T

E

E

E

L

T

L

A

E

K

L

I

T

L

G

D

L

L

S

S

G

E

M

L

E

L

K

D

R

R

Y

K

P

P

N

G

Q
|
Q

L

L

S

S

G
|
G

Q
|
Q

R

R

Q

Q

R

R

V

V

A

A

F

M

A

G

R

R

A

A

L

I

A

V

P

R

N

S

P

P

Q

K

V

A

L

F

L

L

L

M

D

D

E

Q

P

P

F

L

A

S

A

N

I

L

D

D

A

A

K

K

V

L

R

R

T

V

E

Q

L

M

R

R

N

A

W

E

L

I

R

S

E

R

M

L

V

Q

V

D

K

R

L

L

G

G

I

T

T

T

S

T

I

V

F

Y

V

V

T

T

H
|
H

D

D

Q

Q

D

T

E

E

A

A

V

M

E

T

V

L

A

G

D

D

E

R

I

V

T

M

N

L

H

A

G

G

H

E

I

V

E

Q

Q

Q

M

I

G

G

T

T

P

P

M

D

E

E

I

L

Y

Y

K

S

S

S

P

P

D

A

T

N

P

L

F

F

V

V

A

A

Q

G

F

F

I

I

G

G

R

S

S

P

S

A

I

M

binding adenosine-5'-triphosphate: Y12 (= Y12), S38 (= S37), G39 (= G38), G41 (= G40), K42 (= K41), S43 (≠ T42), Q82 (= Q81), Q133 (= Q132), G136 (= G135), G137 (= G136), Q138 (= Q137), H192 (= H191)
binding magnesium ion: S43 (≠ T42), Q82 (= Q81)

8hplC Lpqy-sugabc in state 1 (see paper)
45% identity, 66% coverage: 8:238/352 of query aligns to 8:237/384 of 8hplC

query
sites
8hplC

I

V

C

T

K

K

N

S

Y
|
Y

G

P

N

R

F

-

R

A

A

A

S

V

D

K

H

E

V

F

S

S

F

M

G

T

I

I

E

A

K

D

G

G

K

E

L

F

V

I

A

I

L

L

L

V

G

G

P

P

S

S

G
|
G

S
x
C

G
|
G

K
|
K

T
x
S

T
|
T

L

T

L

L

R

N

M

M

I

I

A

A

G

G

L

L

E

E

T

E

P

I

N

T

S

S

G

G

D

E

I

L

Y

R

I

I

D

G

G

G

Q

E

R

R

V

V

N

N

D

E

V

K

P

A

A

P

A

K

K

D

R

R

G

D

I

I

G

A

F

M

V

V

F

F

Q

Q

N

S

Y

Y

A

A

L

L

F

Y

R

P

Y

H

M

M

T

T

V

V

Y

R

D

Q

N

N

V

I

A

A

F

F

G

P

L

L

E

T

L

L

A

A

K

K

V

V

P

P

K

K

K

A

E

E

I

I

K

A

R

K

V

V

T

E

E

E

L

T

L

A

E

K

L

I

T

L

G

D

L

L

S

S

G

E

M

L

E

L

K

D

R

R

Y

K

P

P

N

G

Q

Q

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

F

M

A

G

R

R

A

A

L

I

A

V

P

R

N

S

P

P

Q

K

V

A

L

F

L

L

L

M

D

D

E

Q

P

P

F

L

A

S

A

N

I

L

D

D

A

A

K

K

V

L

R

R

T

V

E

Q

L

M

R

R

N

A

W

E

L

I

R

S

E

R

M

L

V

Q

V

D

K

R

L

L

G

G

I

T

T

T

S

T

I

V

F

Y

V

V

T

T

H

H

D

D

Q

Q

D

T

E

E

A

A

V

M

E

T

V

L

A

G

D

D

E

R

I

V

T

M

N

L

H

A

G

G

H

E

I

V

E

Q

Q

Q

M

I

G

G

T

T

P

P

M

D

E

E

I

L

Y

Y

K

S

S

S

P

P

D

A

T

N

P

L

F

F

V

V

A

A

Q

G

F

F

I

I

G

G

R

S

S

P

S

A

I

M

binding adenosine-5'-triphosphate: Y12 (= Y12), G37 (= G38), C38 (≠ S39), G39 (= G40), K40 (= K41), S41 (≠ T42), T42 (= T43)

P9WQI3 Trehalose import ATP-binding protein SugC; MtbSugC; Nucleotide-binding domain of SugABC transporter; NBD of SugABC transporter; SugABC transporter ATPase SugC; EC 7.5.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
45% identity, 62% coverage: 21:238/352 of query aligns to 23:240/393 of P9WQI3

query
sites
P9WQI3

V

L

S

N

F

L

G

T

I

I

E

A

K

D

G

G

K

E

L

F

V

L

A

I

L

L

L

V

G

G

P

P

S

S

G

G

S

C

G

G

K

K

T

T

L

T

L

L

R

N

M

M

I

I

A

A

G

G

L

L

E

E

T

D

P

I

N

S

S

S

G

G

D

E

I

L

Y

R

I

I

D

A

G

G

Q

E

R

R

V

V

N

N

D

E

V

K

P

A

A

P

A

K

K

D

R

R

G

D

I

I

G

A

F

M

V

V

F

F

Q

Q

N

S

Y

Y

A

A

L

L

F

Y

R

P

Y

H

M

M

T

T

V

V

Y

R

D

Q

N

N

V

I

A

A

F

F

G

P

L

L

E

T

L

L

A

A

K

K

V

M

P

R

K

K

K

A

E

D

I

I

K

A

K

Q

R

K

V

V

T

S

E

E

L

T

L

A

E

K

L

I

T

L

G

D

L

L

S

T

G

N

M

L

E

L

K

D

R

R

Y

K

P

P

N

S

Q

Q

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

F

M

A

G

R

R

A

A

L

I

A

V

P

R

N

H

P

P

Q

K

V

A

L

F

L

L

L

M

D

D

E

E

P

P

F

L

A

S

A

N

I

L

D

D

A

A

K

K

V

L

R

R

T

V

E

Q

L

M

R

R

N

G

W

E

L

I

R

A

E

Q

M

L

V

Q

V

R

K

R

L

L

G

G

I

T

T

T

S

T

I

V

F

Y

V

V

T

T

H
|
H

D

D

Q

Q

D

T

E

E

A

A

V

M

E

T

V

L

A

G

D

D

E

R

I

V

T

M

N

Y

H

G

G

G

H

I

I

A

E

Q

Q

Q

M

I

G

G

T

T

P

P

M

E

E

E

I

L

Y

Y

K

E

S

R

P

P

D

A

T

N

P

L

F

F

V

V

A

A

Q

G

F

F

I

I

G

G

R

S

S

P

S

A

I

M

H193 (= H191) mutation to A: Decreased hydrolysis of ATP. No change in KM, but 2-fold reduction in Vmax compared to wild-type.

3d31A Modbc from methanosarcina acetivorans (see paper)
34% identity, 91% coverage: 3:322/352 of query aligns to 2:336/348 of 3d31A

query
sites
3d31A

V

I

E

E

M

I

R

E

D

S

I

L

C

S

K

R

N

K

Y

W

G

K

N

N

F

F

R

-

A

S

S

L

D

D

H

N

V

L

S

S

F

L

G

K

I

V

E

E

K

S

G

G

K

E

L

Y

V

F

A

V

L

I

L

L

G

G

P

P

S

T

G

G

S

A

G

G

K

K

T

T

T

L

L

F

L

L

R

E

M

L

I

I

A

A

G

G

L

F

E

H

T

V

P

P

N

D

S

S

G

G

D

R

I

I

Y

L

I

L

D

D

G

G

Q

K

R

D

V

V

N

T

D

D

V

L

P

S

A

P

A

E

K

K

R

H

G

D

I

I

G

A

F

F

V

V

F

Y

Q

Q

N

N

Y

Y

A

S

L

L

F

F

R

P

Y

H

M

M

T

N

V

V

Y

K

D

K

N

N

V

L

A

E

F

F

G

G

L

M

E

R

L

M

A

K

K

K

V

I

P

K

K

D

K

P

E

-

I

-

K

-

K

K

R

R

V

V

T

L

E

D

L

T

L

A

E

R

L

D

T

L

G

K

L

I

S

E

G

H

M

L

E

L

K

D

R

R

Y

N

P

P

N

L

Q

T

L

L

S

S

G

G

Q

E

R

Q

Q

Q

R

R

V

V

A

A

F

L

A

A

R

R

A

A

L

L

A

V

P

T

N

N

P

P

Q

K

V

I

L

L

D

D

E

E

P

P

F

L

A

S

A

A

I

L

D

D

A

P

K

R

V

T

R

Q

T

E

E

N

L

A

R

R

N

E

W

M

L

L

R

S

E

V

M

L

V

H

V

K

K

K

L

N

G

K

I

L

T

T

S

V

I

L

F

H

V

I

T

T

H

H

D

D

Q

Q

D

T

E

E

A

A

V

R

E

I

V

M

A

A

D

D

E

R

I

I

I

A

I

V

T

V

N

M

H

D

G

G

H

K

I

L

E

I

Q

Q

M

V

G

G

T

K

P

P

M

E

E

E

I

I

Y

F

K

E

S

K

P

P

D

V

T

E

P

G

F

R

V

V

A

A

Q

S

F

F

I

V

G

G

-

F

-

E

-

N

-

V

-

L

R

K

S

G

S

R

I

V

V

I

E

S

H

A

Y

E

E

Q

R

G

L

L

-

R

-

I

-

R

-

V

-

G

-

E

-

V

-

I

-

D

-

A

G

G

F

D

D

M

P

E

V

V

E

G

G

D

A

Q

T

V

H

Y

A

A

V

F

I

L

R

R

P

P

E

E

F

N

V

I

R

A

M

L

S

S

R

K

S

S

E

S

P

T

P

Q

Q

S

H
x
S

M

I

S

R

A

N

A

S

E

L

R

Q

G

G

I

R

V

V

K

T

D

E

V

A

I

W

F

V

R

L

G

G

N

A

H

L

L

V

D

R

V

V

V

K

V

V

D

D

V

C

G

-

G

G

I

V

P

P

-

L

-

N

-

V

I

L

V

I

T
|
T

E

R

R

R

S
|
S

L

A

E

E

K

E

D

M

P

E

V

L

E

S

I

P

G

G

E

V

Q

Q

V

I

Y

Y

binding tungstate(vi)ion: S286 (≠ H274), T320 (= T306), S323 (= S309)

Sites not aligning to the query:

binding tungstate(vi)ion: 340, 341, 342

1oxvD Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
34% identity, 94% coverage: 5:334/352 of query aligns to 2:353/353 of 1oxvD

query
sites
1oxvD

M

V

R

R

D

I

I

I

C

V

K

K

N

N

Y

V

-

S

-

K

-

V

-
x
F

-

K

G

G

N

K

F

V

R

V

A

A

S

L

D

D

H

N

V

V

S

N

F

I

G

N

I

I

E

E

K

N

G

G

K

E

L

R

V

F

A

G

L

I

L

L

G

G

P

P

S
|
S

G
|
G

S

A

G
|
G

K
|
K

T
|
T

L

F

L

M

R

R

M

I

I

I

A

A

G

G

L

L

E

D

T

V

P

P

N

S

S

T

G

G

D

E

I

L

Y

Y

I

F

D

D

G

D

Q

R

R

L

V

V

N

A

D

S

-

N

-

G

-

K

-

L

-

I

V

V

P

P

A

P

A

E

K

D

R

R

G

K

I

I

G

G

F

M

V

V

F

F

Q
|
Q

N

T

Y

W

A

A

L

L

F

Y

R

P

Y

N

M

L

T

T

V

A

Y

F

D

E

N

N

V

I

A

A

F

F

G

P

L

L

E

T

L

N

A

M

K

K

V

M

P

S

K

K

K

E

E

E

I

I

K

R

K

K

R

R

V

V

T

E

E

E

L

V

L

A

E

K

L

I

T

L

G

D

L

I

S

H

G

H

M

V

E

L

K

N

R

H

Y

F

P

P

N

R

Q

E

L

L

S

S

G

G

Q

Q

R

Q

Q

Q

R

R

V

V

A

A

F

L

A

A

R

R

A

A

L

L

A

V

P

K

N

D

P

P

Q

S

V

L

L

L

D

D

E
|
E

P

P

F

F

A

S

A

N

I

L

D

D

A

A

K

R

V

M

R

R

T

D

E

S

L

A

R

R

N

A

W

L

L

V

R

K

E

E

M

V

V

Q

V

S

K

R

L

L

G

G

I

V

T

T

S

L

I

L

F

V

V

V

T

S

H

H

D

D

Q

P

D

A

E

D

A

I

V

F

E

A

V

I

A

A

D

D

E

R

I

V

I

G

I

V

T

L

N

V

H

K

G

G

H

K

I

L

E

V

Q

Q

M

V

G

G

T

K

P

P

M

E

E

D

I

L

Y

Y

K

D

S

N

P

P

D

V

T

S

P

I

F

Q

V

V

A

A

Q

S

F

L

I

I

G

G

R

E

S

I

S

N

I

E

V

L

E

E

H

G

Y

K

E

V

R

T

-

N

-

E

-

G

-

V

-

I

-

G

L

S

L

L

G

R

F

F

D

P

P

V

V

S

E

V

G

S

A

S

T

D

H

R

A

A

V

I

-

I

-

G

I

I

R

R

P

P

E

E

F

D

V

V

R

K

M

L

S

S

R

K

S

D

E

V

-

I

P

K

P

D

Q

D

H

S

M

W

S

I

A

L

A

V

E

G

R

K

G

G

I

K

V

V

K

K

D

V

V

I

I

G

F

Y

R

Q

G

G

N

G

H

L

L

F

D

R

V

I

V

T

V

I

D

-

V

-

G

-

I

T

P

P

I

L

V

D

T

S

E

E

R

E

S

E

L

I

-

F

-

T

-

Y

E

S

K

D

D

H

P

P

V

I

E

H

I

S

G

G

E

E

Q

E

-

V

-

L

V

V

Y

Y

V

V

L

R

I

K

Y

D

R

K

L

I

Y

K

I

V

F

F

D

E

E

K

N

N

binding phosphoaminophosphonic acid-adenylate ester: F13 (vs. gap), S40 (= S37), G41 (= G38), G43 (= G40), K44 (= K41), T45 (= T42), T46 (= T43), Q89 (= Q81), E166 (= E158)
binding magnesium ion: T45 (= T42), Q89 (= Q81)

1oxvA Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
34% identity, 94% coverage: 5:334/352 of query aligns to 2:353/353 of 1oxvA

query
sites
1oxvA

M

V

R

R

D

I

I

I

C

V

K

K

N

N

Y

V

-

S

-

K

-

V

-
x
F

-

K

G

G

N

K

F

V

R

V

A

A

S

L

D

D

H

N

V

V

S

N

F

I

G

N

I

I

E

E

K

N

G

G

K

E

L

R

V

F

A

G

L

I

L

L

G

G

P

P

S
|
S

G
|
G

S

A

G
|
G

K
|
K

T
|
T

L

F

L

M

R

R

M

I

I

I

A

A

G

G

L

L

E

D

T

V

P

P

N

S

S

T

G

G

D

E

I

L

Y

Y

I

F

D

D

G

D

Q

R

R

L

V

V

N

A

D

S

-

N

-

G

-

K

-

L

-

I

V

V

P

P

A

P

A

E

K

D

R

R

G

K

I

I

G

G

F

M

V

V

F

F

Q

Q

N

T

Y

W

A

A

L

L

F

Y

R

P

Y

N

M

L

T

T

V

A

Y

F

D

E

N

N

V

I

A

A

F

F

G

P

L

L

E

T

L

N

A

M

K

K

V

M

P

S

K

K

K

E

E

E

I

I

K

R

K

K

R

R

V

V

T

E

E

E

L

V

L

A

E

K

L

I

T

L

G

D

L

I

S

H

G

H

M

V

E

L

K

N

R

H

Y

F

P

P

N

R

Q

E

L

L

S

S

G

G

Q

Q

R

Q

Q

Q

R

R

V

V

A

A

F

L

A

A

R

R

A

A

L

L

A

V

P

K

N

D

P

P

Q

S

V

L

L

L

D

D

E
|
E

P

P

F

F

A

S

A

N

I

L

D

D

A

A

K

R

V

M

R

R

T

D

E

S

L

A

R

R

N

A

W

L

L

V

R

K

E

E

M

V

V

Q

V

S

K

R

L

L

G

G

I

V

T

T

S

L

I

L

F

V

V

V

T

S

H

H

D

D

Q

P

D

A

E

D

A

I

V

F

E

A

V

I

A

A

D

D

E

R

I

V

I

G

I

V

T

L

N

V

H

K

G

G

H

K

I

L

E

V

Q

Q

M

V

G

G

T

K

P

P

M

E

E

D

I

L

Y

Y

K

D

S

N

P

P

D

V

T

S

P

I

F

Q

V

V

A

A

Q

S

F

L

I

I

G

G

R

E

S

I

S

N

I

E

V

L

E

E

H

G

Y

K

E

V

R

T

-

N

-

E

-

G

-

V

-

I

-

G

L

S

L

L

G

R

F

F

D

P

P

V

V

S

E

V

G

S

A

S

T

D

H

R

A

A

V

I

-

I

-

G

I

I

R

R

P

P

E

E

F

D

V

V

R

K

M

L

S

S

R

K

S

D

E

V

-

I

P

K

P

D

Q

D

H

S

M

W

S

I

A

L

A

V

E

G

R

K

G

G

I

K

V

V

K

K

D

V

V

I

I

G

F

Y

R

Q

G

G

N

G

H

L

L

F

D

R

V

I

V

T

V

I

D

-

V

-

G

-

I

T

P

P

I

L

V

D

T

S

E

E

R

E

S

E

L

I

-

F

-

T

-

Y

E

S

K

D

D

H

P

P

V

I

E

H

I

S

G

G

E

E

Q

E

-

V

-

L

V

V

Y

Y

V

V

L

R

I

K

Y

D

R

K

L

I

Y

K

I

V

F

F

D

E

E

K

N

N

binding phosphoaminophosphonic acid-adenylate ester: F13 (vs. gap), S40 (= S37), G41 (= G38), G43 (= G40), K44 (= K41), T45 (= T42), T46 (= T43), E166 (= E158)

Query Sequence

>WP_022046731.1 NCBI__GCF_001406815.1:WP_022046731.1
MYVEMRDICKNYGNFRASDHVSFGIEKGKLVALLGPSGSGKTTLLRMIAGLETPNSGDIY
IDGQRVNDVPAAKRGIGFVFQNYALFRYMTVYDNVAFGLELAKVPKKEIKKRVTELLELT
GLSGMEKRYPNQLSGGQRQRVAFARALAPNPQVLLLDEPFAAIDAKVRTELRNWLREMVV
KLGITSIFVTHDQDEAVEVADEIIITNHGHIEQMGTPMEIYKSPDTPFVAQFIGRSSIVE
HYERLLGFDPVEGATHAVIRPEFVRMSRSEPPQHMSAAERGIVKDVIFRGNHLDVVVDVG
GIPIVTERSLEKDPVEIGEQVYVLIYRLYIFDENQTYLVENKEMQEGEVFYI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory