SitesBLAST

K156 (≠ G163) mutation K->A,Q: Retains less than 10% of wild-type activity.; mutation to R: Retains 40% of wild-type activity.
F212 (= F216) mutation F->A,Q: Loss of activity.; mutation to Y: No change in activity.
E218 (= E222) mutation E->A,Q: Loss of activity.; mutation to D: Retains 70% of wild-type activity.

Sites not aligning to the query:

27 mutation T->A,S: Retains 60% of wild-type activity.; T→N: Retains 20% of wild-type activity.
98 E→A: Retains 80% of wild-type activity.

P39277 L-methionine/branched-chain amino acid exporter YjeH from Escherichia coli (strain K12) (see paper)
22% identity, 52% coverage: 45:334/554 of query aligns to 44:317/418 of P39277

query
sites
P39277

S

A

W

W

V

P

L

V

M

L

T

I

F

I

L

L

Y

-

V

V

I

F

P

P

Y

I

S

A

L

I

I

V

V

F

A

A

H

I

L

L

G

G

S

R

T

H

F

Y

S

P

A

-

D

S

A

A

G

G

I

V

T

A

S

H

W

F

V

V

R

G

E

M

T

A

N

F

G

G

D

S

T

R

L

L

G

E

Y

R

F

V

A

T

A

G

W

W

F

L

Y

F

W

L

I

S

T

V

-

I

-

P

-

V

G

G

L

L

P

P

Y

A

V

A

V

L

D

Q

V

I

A

A

N

A

S

G

V

F

V

G

I

Q

S

A

-

M

F

F

G

G

W

W

M

-

V

-

N

-

G

-

D

-

G

-

D

-

I

-

Q

-

S

-

H

-

M

-

N

-

N

H

A

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W

W

F

Q

G

L

L

L

T

A

A

E

V

L

V

G

F

T

I

L

F

A

F

L

I

V

W

W

F

Y

-

I

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S

T

R

R

F

G

Q

A

N

S

K

S

S

S

L

A

E

N

I

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V

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I

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A

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A

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-

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-

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V

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F

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G

A

M

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I

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A

G

G

L

-

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-

Q

-

G

-

S

-

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-

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-

K

D

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I

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K

P

P

F

A

N

N

L

I

Q

P

A

F

F

P

L

A

P

P

K

G

K

N

L

I

D

E

L

L

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T

-

G

F

L

F

F

A

A

T

A

F

L

G

S

L

V

F

M

I

F

F
x
W

A

C

M

F

N

V

G

G

S

L

E

E

L

A

A

F

A

A

P

H

Y

L

T

A

A

S

D

E

M

F

K

K

H

N

P

P

S

E

R

R

D

D

F

F

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P

K

R

S

A

L

L

K

-

M

M

I

I

A

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M

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M

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Y

F

W

G

G

T

C

F

T

S

V

L

V

G

V

V

L

Y

H

F

F

N

D

A

A

H

Y

H

-

L

-

P

-

N

-

D

G

L

E

K

K

M

M

N

A

G

A

S

A

Y

A

Y

S

A

L

F

P

Q

K

A

I

I

V

G

V

K

Q

Q

L

F

F

G

G

L

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G

G

N

A

I

-

L

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M

L

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F

A

A

C

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V

V

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G

C

Y

I

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Y

A

M

C

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F

A

A

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L

L

A

N

V

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I

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L

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D

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A

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S

F

T

A

R

R

V

L

-

V

-

W

-

S

-

Q

-

A

-

Q

-

H

-

N

-

P

-

D

-

H

F

Y

L

L

G

A

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M

L

A

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K

S

R

R

F

H

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P

R

N

Q

N

W195 (≠ F216) mutation to A: Strong decrease in methionine efflux.

Sites not aligning to the query:

24 T→Y: Strong decrease in methionine efflux.
25 G→F: Strong decrease in methionine efflux.

P25737 Lysine-specific permease LysP; Lysine transporter LysP; Trigger transporter LysP from Escherichia coli (strain K12) (see 2 papers)
23% identity, 34% coverage: 216:403/554 of query aligns to 216:397/489 of P25737

query
sites
P25737

F
|
F

A

S

M

F

N

Q

G

G

S

T

E
|
E

L

L

A

I

A

G

P

I

Y

A

T

A

A

G

D
x
E

M

S

K

E

H

D

P

P

S

A

R

K

D

N

F

I

P

P

K

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S

A

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K

R

M

Q

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A

F

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W

M

R

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I

M

L

F

L

L

F

T

Y

L

V

F

F

G

A

T

I

F

L

S

I

L

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G

S

V

L

-

I

-

P

Y

Y

F

T

N

D

A

P

H

S

H

L

L

L

P

R

N

N

D
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D

L

V

K

K

M

-

N
x
D

G

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S

Y

V

Y

S

A

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F

F

Q

T

A

L

I

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G

F

K

Q

Q

H

F

A

G

G

L

L

G

-

N

-

I

-

L

-

M

L

Y

S

V

A

F

A

A

A

V

V

V

M

Q

N

C

A

I

V

Y

I

M

L

L

T

A

A

Q

V

L

L

A

S

V

A

I

G

-

N

-

S

-

G

L

M

D

Y

A

A

S

S

T

T

R

R

V

M

F

L

L

Y

G

T

D

L

M

A

A

C

K

D

R

G

F

K

M

A

P

P

R

R

Q

I

L

F

S

A

K

K

T

L

N

S

A

R

A

G

G

G

L

V

P

P

I

R

N

N

G

A

Y

L

W

Y

M

A

T

T

I

V

L

I

C

A

G

G

I

L

I

C

M

F

V

L

I

T

A

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G

M

F

F

L

G

P

N

Q

Q

I

-

N

-

D

T

I

V

F

Y

N

L

W

W

L

L

N

N

L

T

N

S

G

G

I

M

V

-

S

-

P

-

F

-

S

-

T

T

C

G

F

F

L

I

F

A

W

W

A

L

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G

M

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M

A

V

I

R

S

R

H

H

Y

S

R

D

F

R

R

F216 (= F216) mutation to L: Retains 13% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
E222 (= E222) mutation to A: Abolishes lysine uptake. Strongly inhibits CadC.
E230 (≠ D230) mutation to V: Abolishes lysine uptake. Shows significant less inhibition of CadC.
D275 (= D272) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-278.
D278 (≠ N276) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-275.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
102 Y→L: Retains 4% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
106 W→L: Retains 20% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
163 K→A: Retains 24% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
438 E→A: Retains 14% of wild-type lysine uptake activity. Is unable to inhibit CadC.
443 D→A: Retains 11% of wild-type lysine uptake activity. Is unable to inhibit CadC.
446 D→A: Retains 13% of wild-type lysine uptake activity. Is unable to inhibit CadC.

P63235 Glutamate/gamma-aminobutyrate antiporter; Glu/GABA antiporter; Extreme acid sensitivity protein from Escherichia coli (strain K12) (see 2 papers)
19% identity, 59% coverage: 34:361/554 of query aligns to 34:355/511 of P63235

query
sites
P63235

N

T

F

F

R

A

N

T

Q

S

G

G

L

F

V

S

A

L

V

V

T

F

S

F

W

L

V

L

L

L

M

G

T

G

F

I

L

L

Y

W

V

F

I

I

P

P

Y

V

S

G

L

L

I

C

V

A

A

A

H

E

L

M

G

A

S

T

T

V

F

D

S

G

A

W

D

E

A

E

G

G

I

V

T

F

S

A

W

W

V

V

R

S

E

N

T

T

N

L

G

G

D

P

T

R

L

W

G

G

Y

F

F

A

A

A

A

I

W

S

F

F

Y

G

W

Y

-

L

-

Q

-

I

-

A

I

I

T

G

G

F

L

I

P

P

Y

M

V

L

V

Y

D

F

V

V

A

L

N

G

S

A

V

L

V

S

I

Y

S

I

F

L

G

K

W

W

M

P

V

A

N

L

G

N

D

E

G

D

D

P

I

I

Q

T

S

K

H

T

M

I

N

-

A

-

W

-

F

-

G

-

L

-

T

-

A

A

V

A

V

L

F

I

I

I

F

L

F

W

I

A

W

L

F

A

Q

L

S

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R

Q

F

F

-

G

Q

T

N

K

K

Y

S

T

L

A

E

R

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I

M

A

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K

V

V

I

G

G

F

G

A

A

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M

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F

L

I

L

M

P

T

A

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F

L

I

F

L

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A

M

L

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A

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A

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G

Y

L

L

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G

G

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A

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A

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P

-

F

M

N

D

L

S

Q

K

A

T

F

F

L

F

P

P

K

D

K

F

L

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D

K

L

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S

G

F

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F

L

A

V

T

V

F

F

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V

L

A

F

F

I

I

F
x
L

A

S

M

Y

N

M

G

G

S

V

E
|
E

L

A

A

S

A

A

P

T

Y

H

T

V

A

N

D

E

M

M

K

S

H

N

P

P

S

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R

R

D

D

F

Y

P

P

K

L

S

A

L

M

K

L

M

L

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L

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M

V

V

M

A

T

A

M

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F

C

L

L

T

S

L

S

F

V

G

G

T

G

F

L

S

S

L

I

G

A

V

M

Y

V

F

I

N

P

A

G

H

-

L

-

P

-

N

N

D

E

L

I

K

N

M

L

N

S

-

A

G

G

S

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Y

M

Y

Q

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F

F

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F

-

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-

L

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A

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L

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Y

W

V

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F

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A

R

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V

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A

I

L

Y

L

M

L

L

L

A

G

Q

V

L

L

A

A

V
x
E

I

I

L

A

D

S

-
x
W

-

I

-

V

A

G

S

P

T

S

R

R

V

G

F

M

L

Y

G

V

D

T

M

A

A

Q

K

K

R

N

F

L

M

L

P

P

R

A

Q

A

L

F

S

A

K

K

T

M

N

N

A

K

A

N

G

G

L

V

P

P

I

V

N

T

G

L

Y

V

W

I

M

S

T

Q

T

L

I

V

L

I

C

T

G

S

I

I

I

A

M

L

V

I

I

I

L212 (≠ F216) mutation to A: 70% decrease in substrate transport.
E218 (= E222) mutation to A: At least 90% decrease in substrate transport.
E304 (≠ V313) mutation to A: At least 90% decrease in substrate transport.
W308 (vs. gap) mutation to A: At least 90% decrease in substrate transport.

Sites not aligning to the query:

25 M→A: 25% decrease in substrate transport.
30 Y→A: At least 90% decrease in substrate transport.
378 Y→A: At least 90% decrease in substrate transport.
382 Y→A: At least 90% decrease in substrate transport.
471:511 mutation Missing: Shifts the pH-dependent substrate transport towards higher pH values. Transports Gln, but not Glu, at pH 7.0 or higher.
491 H→A: Allows substrate transport at pH 6.5.
497 R→A: Allows substrate transport at pH 6.5.
499 R→A: Allows substrate transport at pH 6.5.
502 H→A: Allows substrate transport at pH 6.5.
503 Y→A: Allows substrate transport at pH 6.5.

Query Sequence

>WP_022528465.1 NCBI__GCF_000469325.1:WP_022528465.1
MPDTTETRPHYIPWVVVALMDFVTVIGFDDIIYNFRNQGLVAVTSWVLMTFLYVIPYSLI
VAHLGSTFSADAGGITSWVRETNGDTLGYFAAWFYWITGLPYVVDVANSVVISFGWMVNG
DGDIQSHMNNAWFGLLTAVVFIFFIWFQSRFQNKSLEIMSVIGGGAMFIMTVLFVGMTIV
GLTQGSPIKTQPFNLQAFLPKKLDLSFFATFGLFIFAMNGSELAAPYTADMKHPSRDFPK
SLKMIAVMTMFLTLFGTFSLGVYFNAHHLPNDLKMNGSYYAFQAIGKQFGLGNILMYVFA
VVQCIYMLAQLAVILDASTRVFLGDMAKRFMPRQLSKTNAAGLPINGYWMTTILCGIIMV
IAGFLPQINDIFNWLLNLNGIVSPFSTCFLFWAFMMVRRHSDRYPTPSYTYLKNDRVGFL
VGLWMFAITFLFALMGFWPVDAAYGSALWTRSLVLYIVVTLGMVVLGFLMPWIAKRQRER
DGLAFGAAFWYTVTGLLMVGGVYVFTIGIYNEIVPASFGVWRVVITVLVDLGVVALVWLG
TRNGRRQVATAADD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory