SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_022529984.1 NCBI__GCF_000469325.1:WP_022529984.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

5eyfB Crystal structure of solute-binding protein from enterococcus faecium with bound glutamate
64% identity, 86% coverage: 29:264/274 of query aligns to 1:236/243 of 5eyfB

query
sites
5eyfB

A

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binding glutamic acid: S65 (= S93), R68 (= R96), T83 (= T111), M84 (= M112), T85 (= T113), R90 (= R118), S133 (= S161), T134 (≠ N162), Y154 (= Y182), D172 (= D200)

4zv2A An ancestral arginine-binding protein bound to glutamine (see paper)
46% identity, 77% coverage: 54:264/274 of query aligns to 17:225/225 of 4zv2A

query
sites
4zv2A

F

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binding glutamine: F52 (≠ S93), G70 (≠ T111), M71 (= M112), T72 (= T113), R77 (= R118), S120 (= S161), T121 (≠ N162), D159 (= D200)

Sites not aligning to the query:

binding glutamine: 11, 14

4zv1A An ancestral arginine-binding protein bound to arginine (see paper)
47% identity, 77% coverage: 54:263/274 of query aligns to 17:226/226 of 4zv1A

query
sites
4zv1A

F

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binding arginine: F52 (≠ S93), A69 (= A110), G70 (≠ T111), M71 (= M112), T72 (= T113), R77 (= R118), Q117 (= Q159), S120 (= S161), T121 (≠ N162), D161 (= D200)

Sites not aligning to the query:

binding arginine: 11, 14

5t0wA Crystal structure of the ancestral amino acid-binding protein anccdt- 1, a precursor of cyclohexadienyl dehydratase
40% identity, 79% coverage: 46:262/274 of query aligns to 15:228/229 of 5t0wA

query
sites
5t0wA

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binding arginine: D17 (≠ K48), Y20 (≠ T51), W58 (≠ S93), S75 (≠ A110), G76 (≠ T111), M77 (= M112), T78 (= T113), R83 (= R118), Q126 (= Q159), T129 (≠ S161), T130 (≠ N162), D168 (= D200)

6h20A Glnh bound to asn, mycobacterium tuberculosis (see paper)
32% identity, 89% coverage: 20:262/274 of query aligns to 31:271/287 of 6h20A

query
sites
6h20A

L

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binding asparagine: R106 (= R96), T121 (= T111), S123 (≠ T113), R128 (= R118), T170 (≠ S161), T171 (≠ N162), W191 (≠ Y182), D209 (= D200)

6h1uA Glnh bound to asp, mycobacterium tuberculosis (see paper)
32% identity, 89% coverage: 20:262/274 of query aligns to 31:271/287 of 6h1uA

query
sites
6h1uA

L

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R

R

D

I

I

I

F

G

G

Q

V

N

P

V

S

Q

H

I

V

N

E

F

Y

V

R

P

I

V

L

T

S

S

A

Q

A

T

E

R
|
R

I

V

P

T

L

A

L

L

K

Q

N

K

G

S

N

Q

V

V

D

D

A

I

I

V

I

V

A

K

T
|
T

M

M

T
x
S

N

I

T

T

P

C

D

E

R
|
R

R

R

K

K

V

L

V

V

D

N

F

F

S

S

D

T

T

V

Y

Y

F

L

N

D

A

A

G

N

Q

Q

A

R

L

I

L

L

V

A

K

P

K

R

G

D

S

S

P

P

I

I

K

T

S

K

V

V

K

S

D

D

L

L

K

-

K

S

G

G

T

K

K

R

V

V

I

C

G

V

V

A

Q

R

G

G

S
x
T

N
x
T

S

S

V

L

Q

R

N

R

I

I

K

R

K

E

V

I

A

A

P

P

D

P

A

P

Q

V

V

I

L

V

Q

S

L

V

S

V

D

N

Y
x
W

A

A

Q

D

A

C

F

L

T

V

A

A

L

L

Q

Q

S

Q

G

R

Q

E

G

I

D

D

A

A

L

V

T

S

T

T

D
|
D

N

D

G

T

I
|
I

L

L

Y

A

G

G

M

L

S

V

A

E

D

E

N

D

P

P

N

Y

Y

L

V

H

V

I

V

V

G

G

G

P

T

D

F

M

T

A

K

D

E

Q

P

P

Y

Y

A

G

I

V

A

G

V

I

N

N

K

L

G

D

Q

N

K

T

A

G

F

L

L

V

D

R

D

F

V

V

N

N

L

G

A

T

L

L

T

E

S

R

M

I

R

R

Q

N

D

D

G

G

T

T

Y

W

K

N

Q

T

I

L

E

Y

D

R

K

K

W

W

F

L

binding aspartic acid: R106 (= R96), T121 (= T111), S123 (≠ T113), R128 (= R118), T170 (≠ S161), T171 (≠ N162), W191 (≠ Y182), D209 (= D200), I212 (= I203)

6h2tA Glnh bound to glu, mycobacterium tuberculosis (see paper)
32% identity, 89% coverage: 20:262/274 of query aligns to 32:272/288 of 6h2tA

query
sites
6h2tA

L

L

S

R

S

P

C

F

G

A

T

T

K

K

S

A

V

E

A

A

D

D

E

A

D

A

I

V

L

-

S

A

R

D

A

I

K

R

A

A

T

R

N

G

S

R

I

L

T

I

W

V

G

G

V

L

K

D

N

I

D

G

T

S

R

N

L

L

F

F

G

S

L

F

M

R

D

D

I

P

S

I

D

T

G

G

Q

E

I

I

K

T

G

G

F

F

D

D

I

V

D

D

I

I

A

A

K

G

E

E

V

V

S

A

H

R

R

D

I

I

I

F

G

G

Q

V

N

P

V

S

Q

H

I

V

N

E

F

Y

V

R

P

I

V

L

T

S

S

A

Q

A

T

E

R
|
R

I

V

P

T

L

A

L

L

K

Q

N

K

G

S

N

Q

V

V

D

D

A

I

I

V

I

V

A

K

T
|
T

M

M

T
x
S

N

I

T

T

P

C

D

E

R
|
R

R

R

K

K

V

L

V

V

D

N

F

F

S

S

D

T

T

V

Y

Y

F

L

N

D

A

A

G

N

Q

Q

A

R

L

I

L

L

V

A

K

P

K

R

G

D

S

S

P

P

I

I

K

T

S

K

V

V

K

S

D

D

L

L

K

-

K

S

G

G

T

K

K

R

V

V

I

C

G

V

V

A

Q

R

G

G

S
x
T

N
x
T

S

S

V

L

Q

R

N

R

I

I

K

R

K

E

V

I

A

A

P

P

D

P

A

P

Q

V

V

I

L

V

Q

S

L

V

S

V

D

N

Y
x
W

A

A

Q

D

A

C

F

L

T

V

A

A

L

L

Q

Q

S

Q

G

R

Q

E

G

I

D

D

A

A

L

V

T

S

T

T

D
|
D

N

D

G

T

I

I

L

L

Y

A

G

G

M

L

S

V

A

E

D

E

N

D

P

P

N

Y

Y

L

V

H

V

I

V

V

G

G

G

P

T

D

F

M

T

A

K

D

E

Q

P

P

Y

Y

A

G

I

V

A

G

V

I

N

N

K

L

G

D

Q

N

K

T

A

G

F

L

L

V

D

R

D

F

V

V

N

N

L

G

A

T

L

L

T

E

S

R

M

I

R

R

Q

N

D

D

G

G

T

T

Y

W

K

N

Q

T

I

L

E

Y

D

R

K

K

W

W

F

L

binding glutamic acid: R107 (= R96), T122 (= T111), S124 (≠ T113), R129 (= R118), T171 (≠ S161), T172 (≠ N162), W192 (≠ Y182), D210 (= D200)

2v25A Structure of the campylobacter jejuni antigen peb1a, an aspartate and glutamate receptor with bound aspartate (see paper)
35% identity, 74% coverage: 34:237/274 of query aligns to 3:207/231 of 2v25A

query
sites
2v25A

L

L

S

E

R

S

A

I

K

K

A

S

T

K

N

G

S

Q

I

L

T

I

W

V

G

G

V

V

K
|
K

N

N

D

D

T

V

R

P

L

H

F

Y

G

A

L

L

M

L

D

D

I

Q

S

A

D

T

G

G

Q

E

I

I

K

K

G

G

F

F

D

E

I

V

D

D

I

V

A

A

K

K

E

L

V

L

S

A

H

K

R

S

I

I

I

L

G

G

Q

D

N

D

V

K

Q

K

I

I

N

K

F

L

V

V

P

A

V

V

T

N

S

A

Q

K

T

T

R
|
R

I

G

P

P

L

L

L

L

K

D

N

N

G

G

N

S

V

V

D

D

A

A

I

V

I

I

A

A

T
|
T

M

F

T
|
T

N

I

T

T

P

P

D

E

R
|
R

R

K

K

R

V

I

V

Y

D

N

F

F

S

S

D

E

T

P

Y

Y

F

Y

N

Q

A

D

G

A

Q

I

A

G

L

L

L

L

V

V

K

L

K

K

G

E

S

K

P

K

I

Y

K

K

S

S

V

L

K

A

D

D

L

M

K

-

K

K

G

G

T

A

K

N

V

I

I

G

G

V

V

A

Q

Q

G

A

S
x
A

N
x
T

S

T

V

K

Q

K

N

A

I

I

-

G

-

E

-

A

-

A

K

K

K

K

V

I

A

G

P

I

D

D

A

V

Q

K

V

F

L

S

Q

E

L

F

S

P

D

D

Y
|
Y

A

P

Q

S

A

I

F

K

T

A

A

A

L

L

Q

D

S

A

G

K

Q

R

G

V

D

D

A

A

L

F

T

S

T

V

D
|
D

N

K

G

S

I
|
I

L

L

Y

L

G

G

M

Y

S

V

A

D

D

D

N

K

P

S

N

E

Y

-

V

-

V

I

V

L

G

P

G

D

T

S

F

F

T

E

K

P

E

Q

P

S

Y

Y

A

G

I

I

A

V

V

T

N

K

K

K

G

D

Q

D

K

P

A

A

F

F

binding aspartic acid: K17 (= K48), R65 (= R96), T80 (= T111), T82 (= T113), R87 (= R118), A129 (≠ S161), T130 (≠ N162), Y154 (= Y182), D172 (= D200), I175 (= I203)

2yjpA Crystal structure of the solute receptors for l-cysteine of neisseria gonorrhoeae (see paper)
32% identity, 81% coverage: 38:260/274 of query aligns to 8:229/247 of 2yjpA

query
sites
2yjpA

K

K

A

E

T

K

N

G

S

V

I

I

T

R

W

I

G

G

V

V

K
x
F

N

G

D

D

T
x
K

R

P

L

P

F

F

G

G

L

Y

M

V

D

D

I

-

S

A

D

N

G

G

Q

K

I

N

K

Q

G

G

F

F

D

D

I

V

D

E

I

I

A

A

K

K

E

D

V

L

S

A

H

K

R

D

I

L

I

L

G

G

Q

S

N

P

V

D

Q

K

I

V

N

E

F

F

V

V

P

L

V

T

T
x
E

S

A

Q

A

T

N

R
|
R

I

V

P

E

L

Y

L

V

K

R

N

S

G

G

N

K

V

V

D

D

A

L

I

I

I

L

A

A

T
x
N

M
x
F

T
|
T

N

Q

T

T

P

P

D

E

R
|
R

R

A

K

E

V

A

V

V

D

D

F

F

S

A

D

D

T

P

Y

Y

F

M

N

K

A

V

G

A

Q

L

A

G

L

V

L

V

V

S

K

P

K

K

G

N

S

K

P

P

I

I

K

T

S

D

V

M

K

A

D

Q

L

L

K

K

K

D

G

Q

T

T

K

L

V

L

I

V

G

N

V

-

Q

K

G

G

S
x
T

N
x
T

S

A

V

D

Q

A

N

F

I

F

K

T

K

K

V

S

A
x
H

P

P

D
x
E

A

V

Q

K

V

L

L

L

Q

K

L

F

S
x
D

D

Q

Y

N

A

T

Q

E

A

T

F

F

T

D

A

A

L

L

Q

K

S

D

G

G

Q

R

G

G

D

V

A

A

L

L

T

A

T
x
H

D
|
D

N

N

G

A

I

L

L

L

Y

W

G

A

M

W

S

A

A

K

D

E

N

N

P

P

N

N

Y

F

-

E

V

V

V

A

V

I

G

G

G

N

T

L

F

G

T

P

K

A

E

E

P

F

Y

I

A

A

I

P

A

A

V

V

N

Q

K

K

G

G

Q

N

K

A

A

D

F

L

L

L

D

N

D

W

V

V

N

N

L

G

A

E

L

I

T

A

S

A

M

M

R

K

Q

K

D

D

G

G

T

R

Y

L

K

K

Q

A

I

A

E

Y

D

E

K

K

binding cysteine: F18 (≠ K48), K21 (≠ T51), R65 (= R96), N80 (≠ T111), F81 (≠ M112), T82 (= T113), R87 (= R118), T129 (≠ S161), T130 (≠ N162), H167 (≠ T199), D168 (= D200)
binding zinc ion: E61 (≠ T92), H139 (≠ A171), E141 (≠ D173), D148 (≠ S180)

6svfA Crystal structure of the p235gk mutant of argbp from t. Maritima (see paper)
33% identity, 84% coverage: 34:264/274 of query aligns to 3:229/229 of 6svfA

query
sites
6svfA

L

I

S

D

R

E

A

I

K

K

A

S

T

R

N

G

S

Y

I

L

T

L

W

V

G

G

V

L

K

S

N

A

D

D

T
x
F

R

P

L

P

F

F

G

E

L

F

M

V

D

D

I

-

S

E

D

N

G

G

Q

N

I

I

K

V

G

G

F

F

D

D

I

V

D

D

I

L

A

A

K

K

E

E

V

I

S

A

H

R

R

R

I

L

I

-

G

-

Q

-

N

G

V

V

Q

E

I

L

N

K

F

I

V

V

P

D

V

M

T

T

S
x
F

Q

D

T

G

R

L

I

I

P

P

L

S

L

L

K

L

N

T

G

K

N

K

V

I

D

D

A

V

I

I

I

I

A
x
S

T
x
G

M
|
M

T
|
T

N

I

T

T

P

E

D

E

R
|
R

R

K

K

K

V

V

V

V

D

A

F

F

S

S

D

D

T

P

Y

Y

F

F

N

D

A

A

G

G

Q

Q

A

V

L

I

L

V

V

V

K

R

K

K

G

D

S

S

P

D

I

F

-

R

-

P

K

K

S

T

V

Y

K

E

D

D

L

L

K

V

K

G

G

K

T

T

K

V

V

A

-

V

-
x
Q

I

I

G

G

V
x
T

Q
x
T

G

G

S

D

N

I

S

E

V

V

Q

S

N

K

I

Y

K

D

K

G

V

I

A

-

P

-

D

-

A

-

Q

K

V

V

L

V

Q

R

L

F

S

D

D

K

Y

F

A

T

Q

D

A

A

F

F

T

L

A

E

L

L

Q

K

S

R

G

G

Q

R

G

A

D

D

A

A

L

V

T

V

T

L

D
|
D

N

S

G

A

I

T

L

A

Y

R

G

A

M

F

S

V

A

A

D

K

N

N

P

P

N

D

Y

L

V

V

V

I

V

S

G

S

G

G

T

V

F

L

T

S

K

S

E

E

P

Q

Y

Y

A

G

I

I

A

A

V

V

N

R

K

K

G

E

Q

D

K

T

A

D

F

L

L

L

D

E

D

F

V

I

N

N

L

S

A

V

L

L

T

R

S

E

M

L

R

K

Q

K

D

S

G

G

T

K

Y

Y

K

D

Q

V

I

L

E

I

D

E

K

K

W

W

F

F

G

S

N

E

binding arginine: F20 (≠ T51), F58 (≠ S93), S75 (≠ A110), G76 (≠ T111), M77 (= M112), T78 (= T113), R83 (= R118), Q124 (vs. gap), T127 (≠ V158), T128 (≠ Q159), D165 (= D200)

4ymxA Crystal structure of the substrate binding protein of an amino acid abc transporter (see paper)
34% identity, 80% coverage: 43:262/274 of query aligns to 3:222/224 of 4ymxA

query
sites
4ymxA

I

I

T

V

W

M

G

G

V

T

K
x
S

N

A

D
|
D

T
x
F

R

P

L

P

F

F

G

E

L

F

M

H

D

K

I

V

S

E

D

G

G

G

-

K

-

D

Q

E

I

I

K

V

G

G

F

F

D

D

I

I

D

D

I

I

A

A

K

N

E

A

V

I

S

A

H

K

R

K

I

L

I

-

G

-

Q

-

N

G

V

V

Q

K

I

L

N

E

F

I

V

K

P

D

V

M

T

D

S
x
F

Q

K

T

G

R

L

I

I

P

P

L

A

L

L

K

Q

N

A

G

G

N

R

V

V

D

D

A

M

I

V

I

I

A
|
A

T
x
G

M
|
M

T
|
T

N

P

T

T

P

A

D

E

R
|
R

R

K

K

K

V

S

V

V

D

D

F

F

S

S

D

D

T

L

Y

Y

F

Y

N

D

A

S

G

R

Q

Q

A

V

L

V

L

V

V

V

K

K

K

N

G

D

S

S

P

P

I

I

K

S

S

K

V

F

K

D

D

D

L

L

K

K

K

-

G

-

T

V

K

K

V

T

I

I

G

A

V

V

Q
|
Q

-

I

G

G

S
x
T

N
x
T

S

S

V

E

Q

E

N

A

I

A

K

K

K

K

V

I

A

-

P

P

D

N

A

V

Q

K

V

L

L

K

Q

Q

L

L

S

N

D

R

Y

V

A

S

Q

D

A

E

F

F

T

M

A

D

L

L

Q

Q

S

N

G

G

Q

R

G

C

D

D

A

A

L

I

T

V

T

V

D
x
E

N

D

G

T

I

V

-

A

-

K

-

A

-

Y

-

L

-

K

-

E

-

Y

-

K

-

D

L

M

Y

K

G

I

M

L

S

Y

A

M

D

D

N

E

P

I

N

N

Y

N

V

V

V

E

V

N

G

G

G

S

T

-

F

-

T

-

K

-

E

-

P

-

Y

-

A

A

I

V

A

A

V

V

N

A

K

K

G

G

Q

N

K

K

A

S

F

L

L

L

D

D

D

V

V

V

N

N

L

E

A

V

L

I

T

K

S

E

M

L

R

K

Q

Q

D

S

G

G

T

E

Y

Y

K

D

Q

K

I

L

E

V

D

D

K

K

W

W

F

F

binding arginine: S8 (≠ K48), D10 (= D50), F11 (≠ T51), F52 (≠ S93), A69 (= A110), G70 (≠ T111), M71 (= M112), T72 (= T113), R77 (= R118), Q116 (= Q159), T119 (≠ S161), T120 (≠ N162), E157 (≠ D200)

1xt8B Crystal structure of cysteine-binding protein from campylobacter jejuni at 2.0 a resolution (see paper)
29% identity, 83% coverage: 34:260/274 of query aligns to 7:232/251 of 1xt8B

query
sites
1xt8B

L

L

S

D

R

K

A

I

K

K

A

Q

T

N

N

G

S

V

I

V

T

R

W

I

G

G

V

V

K
x
F

N

G

D

D

T
x
K

R

P

L

P

F

F

G

G

L

Y

M

V

D

D

I

-

S

E

D

K

G

G

Q

N

I

N

K

Q

G

G

F

Y

D

D

I

I

D

A

I

L

A

A

K

K

E

R

V

I

S

A

H

K

R

E

I

L

I

F

G

G

Q

D

N

E

V

N

Q

K

I

V

N

Q

F

F

V

V

P

L

V

V

T

E

S

A

Q

A

T

N

R
|
R

I

V

P

E

L

F

L

L

K

K

N

S

G

N

N

K

V

V

D

D

A

I

I

I

I

L

A

A

T
x
N

M

F

T
|
T

N

Q

T

T

P

P

D

Q

R
|
R

R

A

K

E

V

Q

V

V

D

D

F

F

S

C

D

S

T

P

Y

Y

F

M

N

K

A

V

G

A

Q

L

A

G

L

V

L

A

V

V

K

P

K

K

G

D

S

S

P

N

I

I

K

T

S

S

V

V

K

E

D

D

L

L

K

K

K

D

G

K

T

T

K

L

V

L

I

L

G

N

V

-

Q

K

G

G

S
x
T

N
x
T

S

A

V

D

Q

A

N

Y

I

F

K

T

K

Q

V

N

A

Y

P

P

D

N

A

I

Q

K

V

T

L

L

Q

K

L

Y

S

D

D

Q

Y

N

A

T

Q

E

A

T

F

F

T

A

A

A

L

L

Q

M

S

D

G

K

Q

R

G

G

D

D

A

A

L

L

T

S

T
x
H

D
|
D

N

N

G

T

I

L

L

L

Y

F

G

A

M

W

S

V

A

K

D

D

N

H

P

P

N

D

Y

F

V

K

V

M

-

G

V

I

G

K

G

E

T

L

F

G

T

N

K

K

E

D

P

V

Y

I

A

A

I

P

A

A

V

V

N

K

K

K

G

G

Q

D

K

K

A

E

F

L

L

K

D

E

D

F

V

I

N

D

L

N

A

L

L

I

T

I

S

K

M

L

R

G

Q

Q

D

E

G

Q

T

F

Y

F

K

H

Q

K

I

A

E

Y

D

D

K

E

binding cysteine: F21 (≠ K48), K24 (≠ T51), R68 (= R96), N83 (≠ T111), T85 (= T113), R90 (= R118), T132 (≠ S161), T133 (≠ N162), H170 (≠ T199), D171 (= D200)

1wdnA Glutamine-binding protein (see paper)
34% identity, 72% coverage: 66:263/274 of query aligns to 23:219/223 of 1wdnA

query
sites
1wdnA

G

G

F

F

D

D

I

V

D

D

I

L

A

W

K

A

E

A

V

I

S

A

H

K

R

E

I

L

I

-

G

-

Q

-

N

K

V

L

Q

D

I

Y

N

E

F

L

V

K

P

P

V

M

T

D

S
x
F

Q

S

T

G

R

I

I

I

P

P

L

A

L

L

K

Q

N

T

G

K

N

N

V

V

D

D

A

L

I

A

I

L

A

A

T
x
G

M

I

T
|
T

N

I

T

T

P

D

D

E

R
|
R

R

K

K

K

V

A

V

I

D

D

F

F

S

S

D

D

T

G

Y

Y

F

Y

N

K

A

S

G

G

Q

L

A

L

L

V

L

M

V

V

K

K

-

A

K

N

G

N

S

N

P

D

I

V

K

K

S

S

V

V

K

K

D

D

L

L

K

D

K

-

G

-

T

G

K

K

V

V

I

V

G

A

V

V

Q
x
K

-

S

G

G

S
x
T

N

G

S

S

V

V

Q

D

N

Y

I

A

K

K

K

A

V

N

A

I

P

K

D

T

A

K

Q

D

V

L

L

R

Q

Q

L

F

S

P

D

N

Y

I

A

D

Q

N

A

A

F

Y

T

M

A

E

L

L

Q

G

S

T

G

N

Q

R

G

A

D

D

A

A

L

V

T

L

T
x
H

D
|
D

N

T

-

P

G

N

I

I

L

L

Y

Y

G

F

M

I

-

K

S

T

A

A

D

G

N

N

P

G

N

Q

Y

F

V

K

V

A

V

V

G

G

G

D

T

S

F

L

T

E

K

A

E

Q

P

Q

Y

Y

A

G

I

I

A

A

V

F

N

P

K

K

G

G

Q

S

K

D

A

E

F

L

L

R

D

D

D

K

V

V

N

N

L

G

A

A

L

L

T

K

S

T

M

L

R

R

Q

E

D

N

G

G

T

T

Y

Y

K

N

Q

E

I

I

E

Y

D

K

K

K

W

W

F

F

G

G

binding glutamine: F47 (≠ S93), G65 (≠ T111), T67 (= T113), R72 (= R118), K112 (≠ Q159), T115 (≠ S161), H153 (≠ T199), D154 (= D200)

Sites not aligning to the query:

binding glutamine: 10

8vi8A Engineered glutamine binding protein and a cobaloxime ligand - no gln bound (see paper)
34% identity, 72% coverage: 66:263/274 of query aligns to 29:225/227 of 8vi8A

query
sites
8vi8A

G

G

F

F

D

D

I

V

D

D

I

L

A

W

K

A

E

A

V

I

S

A

H

K

R

E

I

L

I

-

G

-

Q

-

N

K

V

L

Q

D

I

Y

N

E

F

L

V

K

P

P

V

M

T

D

S

F

Q

S

T

G

R

I

I

I

P

P

L

A

L

L

K

Q

N

T

G

K

N

N

V

V

D

D

A

L

I

A

I

L

A

A

T

G

M

I

T

T

N

I

T
x
C

P
x
D

D
x
E

R

R

R

K

K

K

V

A

V

I

D

D

F

F

S

S

D

D

T

G

Y

Y

F

Y

N

K

A

S

G

G

Q

L

A

L

L

V

L

M

V

V

K

K

-

A

K

N

G

N

S

N

P

D

I

V

K

K

S

S

V

V

K

K

D

D

L

L

K

D

K

-

G

-

T

G

K

K

V

V

I

V

G

A

V

V

Q

K

-

S

G

G

S

T

N

G

S

S

V

V

Q

D

N

Y

I

A

K

K

K

A

V

N

A

I

P

K

D

T

A

K

Q

D

V

L

L

R

Q

Q

L

F

S

P

D

N

Y

I

A

D

Q

N

A

A

F

Y

T

M

A

E

L

L

Q

G

S

T

G

N

Q

R

G

A

D

D

A

A

L

V

T

L

T

H

D

D

N

T

-

P

G

N

I

I

L

L

Y

Y

G

F

M

I

-

K

S

T

A

A

D

G

N

N

P

G

N

Q

Y

F

V

K

V

A

V

V

G

G

G

D

T

S

F

L

T

E

K

A

E
x
Q

P

Q

Y

Y

A

G

I

I

A

A

V

F

N

P

K
|
K

G
|
G

Q

S

K

D

A

E

F

L

L

R

D

D

D

K

V

V

N

N

L

G

A

A

L

L

T

K

S

T

M

L

R

R

Q

E

D

N

G

G

T

T

Y

Y

K

N

Q

E

I

I

E

Y

D

K

K

K

W

W

F

F

G

G

binding azidobis (dimethylglyoximato) pyridinecobalt: C75 (≠ T115), D76 (≠ P116), E77 (≠ D117), Q186 (≠ E224), K194 (= K232), G195 (= G233)

2vhaA Debp (see paper)
32% identity, 71% coverage: 71:264/274 of query aligns to 48:247/276 of 2vhaA

query
sites
2vhaA

I

I

A

V

K

E

E

A

V

V

S

K

H

K

R

K

I

L

I

N

G

K

Q

P

N

D

V

L

Q

Q

I

V

N

K

F

L

V

I

P

P

V

I

T

T

S
|
S

Q

Q

T

N

R
|
R

I

I

P

P

L

L

L

L

K

Q

N

N

G

G

N

T

V

F

D

D

A

F

I

E

I

C

A

G

T
x
S

M
x
T

T
|
T

N

N

T

N

P

V

D

E

R
|
R

R

Q

K

K

V

Q

V

A

D

A

F

F

S

S

D

D

T

T

Y

I

F

F

N

V

A

V

G

G

Q

T

A

R

L

L

L

L

V

T

K

K

K

K

G

G

S

G

P

D

I

I

K

K

S

D

V

F

K

A

D

D

L

L

K

-

K

K

G

G

T

K

K

A

V

V

I

V

G

V

V

T

Q

S

G

G

S
x
T

N
x
T

S

S

V

E

Q

V

N

L

I

L

K

N

K

K

V

L

A

N

P

E

D

E

A

Q

-

K

-

M

-

N

-

M

Q

R

V

I

L

I

Q

S

L

A

S

K

D

D

Y
x
H

A

G

Q

D

A

S

F

F

T

R

A

T

L

L

Q

E

S

S

G

G

Q

R

G

A

D

V

A

A

L

F

T

M

T
x
M

D
|
D

N

D

G

A

I

L

L

L

Y

A

G

G

-

E

-

R

M

A

S

K

A

A

D

K

N

K

P

P

-

D

N

N

Y

W

V

D

V

I

V

V

G

G

G

K

T

P

F

Q

T

S

K

Q

E

E

P

A

Y

Y

A

G

I

C

A

M

V

L

N

R

K

K

G

D

Q

D

K

P

A

Q

F

F

L

K

D

K

D

L

V

M

N

D

L

D

A

T

L

I

T

A

S

Q

M

V

R

Q

Q

T

D

S

G

G

T

E

Y

A

K

E

Q

K

I

W

E

F

D

D

K

K

W

W

F

F

G

K

N

N

binding glutamic acid: S70 (= S93), R73 (= R96), S88 (≠ T111), T89 (≠ M112), T90 (= T113), R95 (= R118), T137 (≠ S161), T138 (≠ N162), H162 (≠ Y182), M179 (≠ T199), D180 (= D200)

Sites not aligning to the query:

binding glutamic acid: 22

2ia4B Crystal structure of novel amino acid binding protein from shigella flexneri
32% identity, 71% coverage: 71:264/274 of query aligns to 49:248/278 of 2ia4B

query
sites
2ia4B

I

I

A

V

K

E

E

A

V

V

S

K

H

K

R

K

I

L

I

N

G

K

Q

P

N

D

V

L

Q

Q

I

V

N

K

F

L

V

I

P

P

V

I

T

T

S
|
S

Q

Q

T

N

R
|
R

I

I

P

P

L

L

L

L

K

Q

N

N

G

G

N

T

V

F

D

D

A

F

I

E

I

C

A

G

T
x
S

M
x
T

T
|
T

N

N

T

N

P

V

D

E

R
|
R

R

Q

K

K

V

Q

V

A

D

A

F

F

S

S

D

D

T

T

Y

I

F

F

N

V

A

V

G

G

Q

T

A

R

L

L

L

L

V

T

K

K

K

K

G

G

S

G

P

D

I

I

K

K

S

D

V

F

K

A

D

D

L

L

K

-

K

K

G

G

T

K

K

A

V

V

I

V

G

V

V

T

Q

S

G

G

S
x
T

N
x
T

S

S

V

E

Q

V

N

L

I

L

K

N

K

K

V

L

A

N

P

E

D

E

A

Q

-

K

-

M

-

N

-

M

Q

R

V

I

L

I

Q

S

L

A

S

K

D

D

Y
x
H

A

G

Q

D

A

S

F

F

T

R

A

T

L

L

Q

E

S

S

G

G

Q

R

G

A

D

V

A

A

L

F

T

M

T
x
M

D
|
D

N

D

G

A

I

L

L

L

Y

A

G

G

-

E

-

R

M

A

S

K

A

A

D

K

N

K

P

P

-

D

N

N

Y

W

V

D

V

I

V

V

G

G

G

K

T

P

F

Q

T

S

K

Q

E

E

P

A

Y

Y

A

G

I

C

A

M

V

L

N

R

K

K

G

D

Q

D

K

P

A

Q

F

F

L

K

D

K

D

L

V

M

N

D

L

D

A

T

L

I

T

A

S

Q

M

V

R

Q

Q

T

D

S

G

G

T

E

Y

A

K

E

Q

K

I

W

E

F

D

D

K

K

W

W

F

F

G

K

N

N

binding glutamic acid: S71 (= S93), R74 (= R96), S89 (≠ T111), T90 (≠ M112), T91 (= T113), R96 (= R118), T138 (≠ S161), T139 (≠ N162), H163 (≠ Y182), M180 (≠ T199), D181 (= D200)

Sites not aligning to the query:

binding glutamic acid: 23

9e2bC Structure of a solute binding protein from desulfonauticus sp. Bound to l-tryptophan
30% identity, 75% coverage: 57:262/274 of query aligns to 41:248/258 of 9e2bC

query
sites
9e2bC

M

M

D

K

I

D

S

K

D

E

G

G

Q

Q

I

L

K

I

G

G

F

F

D

E

I

I

D

D

I

V

A

A

K

K

E

R

V

L

S

A

H

R

R

D

I

M

I

-

G

-

Q

-

N

G

V

V

Q

K

I

V

N

Q

F

F

V

V

P

P

V

T

T

K

S
x
W

Q

S

T

G

R

I

I

I

P

P

L

A

L

L

K

L

N

T

G

G

N

K

V

F

D

D

A

I

I

I

I

I

A

G

T
x
G

M
|
M

T
x
S

N

I

T

R

P

P

D

D

R
|
R

R

N

K

L

V

K

V

V

D

N

F

F

S

S

D

I

T

P

Y

Y

F

D

N

Y

A

S

G

G

Q

M

A

S

L

L

L

V

V

A

K

N

K

K

-

K

-

L

G

A

S

Q

P

G

I

F

K

S

S

R

V

L

K

E

D

D

L

F

K

N

K

K

G

S

T

E

K

V

V

L

I

I

G

A

V

A

Q

R

-

L

G

G

S
x
T

N
x
T

S

A

V

A

Q

K

N

A

I

A

K

E

K

K

V

Y

A

F

P

P

D

R

A

A

Q

Q

V

L

L

K

Q

L

L

F

S

D

D

D

Y

E

A

A

Q

Q

A

A

F

I

T

Q

A

E

L

L

Q

L

S

N

G

G

Q

R

G

V

D

H

A

A

L

V

T

V

T

A

D

S

N

A

G

P

I

L

L

P

Y

A

G

F

M

K

S

A

A

L

D

E

N

Y

P

P

N

E

-

Q

-

L

Y

F

V

L

V

P

V

I

G

S

G

G

T

T

F

F

T

T

K

K

E

E

P

P

Y

I

A

G

I

F

A

A

V

I

N

R

K

K

G

G

Q

D

K

P

A

D

F

F

L

L

D

N

D

Y

V

L

N

N

L

S

A

W

L

I

T

R

S

V

M

V

R

E

Q

A

D

E

G

G

T

W

Y

L

K

R

Q

E

I

K

E

H

D

H

K

Y

W

W

F

F

binding tryptophan: W74 (≠ S93), G92 (≠ T111), M93 (= M112), S94 (≠ T113), R99 (= R118), T145 (≠ S161), T146 (≠ N162)

Sites not aligning to the query:

binding tryptophan: 39

3vv5A Crystal structure of ttc0807 complexed with (s)-2-aminoethyl-l- cysteine (aec) (see paper)
30% identity, 74% coverage: 63:264/274 of query aligns to 34:232/237 of 3vv5A

query
sites
3vv5A

Q

Q

I

L

K

T

G

G

F

F

D

E

I

V

D

D

I

L

A

G

K

N

E

A

V

I

S

A

H

E

R

R

I

L

I

-

G

-

Q

-

N

G

V

L

Q

K

I

P

N

R

F

W

V

I

P

A

V

Q

T

S

S
x
F

Q

D

T

T

R

L

I

L

P

I

L

Q

L

L

K

N

N

Q

G

G

N

R

V

F

D

D

A

F

I

V

I

I

A
|
A

T
x
S

M

H

T
x
G

N

I

T

T

P

E

D

E

R
|
R

R

A

K

R

V

A

V

V

D

D

F

F

S

T

D

N

T

P

Y

H

F

Y

N

C

A

T

G

G

Q

G

A

V

L

I

L

V

V

S

K

R

K

K

G

G

S

G

P

P

I

-

K

R

S

T

V

A

K

K

D

D

L

L

K

Q

K

-

G

-

T

G

K

K

V

V

I

V

G

G

V

V

Q

Q

-

V

G

G

S
x
T

N
x
T

S
x
Y

V

M

Q

E

N

A

I

A

K

Q

K

K

V

I

A

P

P

G

D

I

A

K

Q

E

V

V

L

R

Q

T

L

Y

S

Q

D

R

Y

D

A

P

Q

D

A

A

F

L

T

Q

A

D

L

L

Q

L

S

A

G

G

Q

R

G

I

D

D

A

T

L

W

T

I

T

T

D

D

N

R

G

F

I

V

L

A

Y

K

G

E

M

A

S

I

A

K

D

E

N

R

-

K

-

L

P

E

N

N

Y

T

V

L

V

Q

V

V

G

G

G

E

T

L

F

V

T

F

K

Q

E

E

P

R

Y

V

A

A

I

M

A

A

V

V

N

A

K

K

G

G

Q

N

K

K

A

S

F

L

L

L

D

D

D

A

V

L

N

N

L

R

A

A

L

L

T

A

S

E

M

L

R

M

Q

Q

D

D

G

G

T

T

Y

Y

K

A

Q

R

I

I

E

S

D

Q

K

K

W

W

F

F

G

G

N

E

binding l-thialysine: F61 (≠ S93), A78 (= A110), S79 (≠ T111), G81 (≠ T113), R86 (= R118), T127 (≠ S161), T128 (≠ N162), Y129 (≠ S163)

Sites not aligning to the query:

binding l-thialysine: 20, 23, 27

3vvfA Crystal structure of ttc0807 complexed with arginine (see paper)
30% identity, 74% coverage: 63:264/274 of query aligns to 38:236/241 of 3vvfA

query
sites
3vvfA

Q

Q

I

L

K

T

G

G

F

F

D

E

I

V

D

D

I

L

A

G

K

N

E

A

V

I

S

A

H

E

R

R

I

L

I

-

G

-

Q

-

N

G

V

L

Q

K

I

P

N

R

F

W

V

I

P

A

V

Q

T

S

S
x
F

Q

D

T

T

R

L

I

L

P

I

L

Q

L

L

K

N

N

Q

G

G

N

R

V

F

D

D

A

F

I

V

I

I

A
|
A

T
x
S

M
x
H

T
x
G

N

I

T

T

P

E

D

E

R
|
R

R

A

K

R

V

A

V

V

D

D

F

F

S

T

D

N

T

P

Y

H

F

Y

N

C

A

T

G

G

Q

G

A

V

L

I

L

V

V

S

K

R

K

K

G

G

S

G

P

P

I

-

K

R

S

T

V

A

K

K

D

D

L

L

K

Q

K

-

G

-

T

G

K

K

V

V

I

V

G

G

V

V

Q
|
Q

-

V

G

G

S
x
T

N
x
T

S
x
Y

V

M

Q

E

N

A

I

A

K

Q

K

K

V

I

A

P

P

G

D

I

A

K

Q

E

V

V

L

R

Q

T

L

Y

S

Q

D

R

Y

D

A

P

Q

D

A

A

F

L

T

Q

A

D

L

L

Q

L

S

A

G

G

Q

R

G

I

D

D

A

T

L

W

T

I

T

T

D

D

N

R

G

F

I

V

L

A

Y

K

G

E

M

A

S

I

A

K

D

E

N

R

-

K

-

L

P

E

N

N

Y

T

V

L

V

Q

V

V

G

G

G

E

T

L

F

V

T

F

K

Q

E
|
E

P

R

Y

V

A

A

I

M

A

A

V

V

N

A

K

K

G

G

Q

N

K

K

A

S

F

L

L

L

D

D

D

A

V

L

N

N

L

R

A

A

L

L

T

A

S

E

M

L

R

M

Q

Q

D

D

G

G

T

T

Y

Y

K

A

Q

R

I

I

E

S

D

Q

K

K

W

W

F

F

G

G

N

E

binding arginine: F65 (≠ S93), A82 (= A110), S83 (≠ T111), H84 (≠ M112), G85 (≠ T113), R90 (= R118), Q128 (= Q159), T131 (≠ S161), T132 (≠ N162), Y133 (≠ S163), E196 (= E224)

Sites not aligning to the query:

binding arginine: 24, 27

3vveA Crystal structure of ttc0807 complexed with lysine (see paper)
30% identity, 74% coverage: 63:264/274 of query aligns to 38:236/241 of 3vveA

query
sites
3vveA

Q

Q

I

L

K

T

G

G

F

F

D

E

I

V

D

D

I

L

A

G

K

N

E

A

V

I

S

A

H

E

R

R

I

L

I

-

G

-

Q

-

N

G

V

L

Q

K

I

P

N

R

F

W

V

I

P

A

V

Q

T

S

S
x
F

Q

D

T

T

R

L

I

L

P

I

L

Q

L

L

K

N

N

Q

G

G

N

R

V

F

D

D

A

F

I

V

I

I

A

A

T
x
S

M
x
H

T

G

N

I

T

T

P

E

D

E

R
|
R

R

A

K

R

V

A

V

V

D

D

F

F

S

T

D

N

T

P

Y

H

F

Y

N

C

A

T

G

G

Q

G

A

V

L

I

L

V

V

S

K

R

K

K

G

G

S

G

P

P

I

-

K

R

S

T

V

A

K

K

D

D

L

L

K

Q

K

-

G

-

T

G

K

K

V

V

I

V

G

G

V

V

Q
|
Q

-

V

G

G

S
x
T

N
x
T

S
x
Y

V

M

Q

E

N

A

I

A

K

Q

K

K

V

I

A

P

P

G

D

I

A

K

Q

E

V

V

L

R

Q

T

L

Y

S

Q

D

R

Y

D

A

P

Q

D

A

A

F

L

T

Q

A

D

L

L

Q

L

S

A

G

G

Q

R

G

I

D

D

A

T

L

W

T

I

T

T

D

D

N

R

G

F

I

V

L

A

Y

K

G

E

M

A

S

I

A

K

D

E

N

R

-

K

-

L

P

E

N

N

Y

T

V

L

V

Q

V

V

G

G

G

E

T

L

F

V

T

F

K

Q

E
|
E

P

R

Y

V

A

A

I

M

A

A

V

V

N

A

K

K

G

G

Q

N

K

K

A

S

F

L

L

L

D

D

D

A

V

L

N

N

L

R

A

A

L

L

T

A

S

E

M

L

R

M

Q

Q

D

D

G

G

T

T

Y

Y

K

A

Q

R

I

I

E

S

D

Q

K

K

W

W

F

F

G

G

N

E

binding lysine: F65 (≠ S93), S83 (≠ T111), H84 (≠ M112), R90 (= R118), Q128 (= Q159), T131 (≠ S161), T132 (≠ N162), Y133 (≠ S163), E196 (= E224)

Sites not aligning to the query:

binding lysine: 24, 27

Query Sequence

>WP_022529984.1 NCBI__GCF_000469325.1:WP_022529984.1
MRKFKSWLWLLPLLAFAVLLSSCGTKSVADEDILSRAKATNSITWGVKNDTRLFGLMDIS
DGQIKGFDIDIAKEVSHRIIGQNVQINFVPVTSQTRIPLLKNGNVDAIIATMTNTPDRRK
VVDFSDTYFNAGQALLVKKGSPIKSVKDLKKGTKVIGVQGSNSVQNIKKVAPDAQVLQLS
DYAQAFTALQSGQGDALTTDNGILYGMSADNPNYVVVGGTFTKEPYAIAVNKGQKAFLDD
VNLALTSMRQDGTYKQIEDKWFGNIAGFSDGGLK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory