SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_022601927.1 NCBI__GCF_000473955.1:WP_022601927.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

7e1sC Crystal structure of dehydrogenase/isomerase fabx from helicobacter pylori in complex with octanoyl-acp (see paper)
36% identity, 96% coverage: 1:344/359 of query aligns to 8:360/366 of 7e1sC

query
sites
7e1sC

M

L

K

K

S

P

F

L

Y

K

M

I

G

G

N

K

L

H

K

T

I

I

D

K

L

F

P

P

V

I

I

F

Q

Q

G
|
G

G
|
G

M
|
M

G
|
G

V

V

G

G

I

I

S

S

L

W

S

D

G

E

L

L

A

A

S

G

A

N

V

V

A

A

N

K

E

E

G

G

G

A

V

L

G

G

V

V

I

I

S

S

C

A

A

V

G

G

L

T

G

G

L

Y

L

Y

Y

K

P

N

K

M

L

R

S

F

T

V

D

E

Y

R

L

I

-

V

-

A

K

K

N

K

S

P

I

F

L

E

G

A

L

L

R

N

E

F

E

Y

L

S

R

K

K

K

A

A

K

L

E

N

K

E

T

I

-

F

-

A

-

N

-

A

-

R

-

K

-

I

-

C

-

G

N

N

G

N

V

P

I

L

G

G

V

A

N
|
N

I

I

M
x
L

V

Y

A

A

L

I

S

N

N

D

F

Y

S

G

D

R

M

V

V

L

K

R

T

D

A

S

I

C

S

E

E

A

K

G

A

A

D

N

I

I

I

I

F

I

S

T

G

G

A
|
A

G
|
G

L
|
L

P

P

L
x
T

D
x
N

L

M

P

P

S

E

Y

F

L

A

E

K

K

D

E

F

S

S

K

D

T

V

K

A

L

L

V

I

P

P

I
|
I

V
x
I

S
|
S

S

S

S

A

R
x
K

A

A

A

L

K

K

L
x
I

I

L

C

C

E
x
K

K
x
R

W

W

R

S

N

D

N
x
R

Y

Y

D

K

Y

R

L

I

P

P

D

D

A

A

I

F

V

I

V

V

E
|
E

G

G

P

P

K

L

A
x
S

G
|
G

G

G

H
|
H

L

Q

G

G

Y

F

K

K

N

Y

D

E

Q

D

I

C

E

F

D

K

E

E

H

E

F

F

S

R

L

L

E

E

H

N

I

L

V

V

P

P

E

K

V

V

I

V

E

E

V

A

I

S

K

K

A

E

Y

W

E

G

K

-

S

-

G

-

H

-

C

-

K

N

I

I

P

P

V

I

I

I

A

A

A

A

G
|
G

G
|
G

L

I

Y

W

T

D

G

R

E

K

D

D

I

I

Y

D

R

T

L

M

M

L

Q

S

L

L

G

G

A

A

T

S

G

G

V

V

Q

Q

L
x
M

G
x
A

S
x
T

K

R

F

F

V
x
L

T

G

T

T

E

K

E

E

C

C

D

D

A

A

S

K

P

-

V

V

F

Y

K

A

Q

D

A

L

Y

L

I

P

D

T

A

L

G

K

Q

K

S

E

D

D

I

I

E

L

I

L

I

I

T

K

S

S

P

P

V

V

G

G

M
x
Y

P

P

G

A

R

R

A

A

I

I

S

N

G

T

E

G

F

V

T

I

R

K

K

R

V

I

K

E

S

E

G

G

S

N

E

A

K

P

P

K

R

I

R

A

C
|
C

A

V

F

S

H
x
N

C
|
C

I
x
V

K

A

T

P

C
|
C

D

N

Y

R

-
x
G

-

E

-

E

-
x
A

T

K

K

K

S

V

P

G

Y

Y

C
|
C

I
|
I

I

A

K

D

A

G

L
|
L

Y

G

N

R

A

S

A

Y

K

L

G

G

N

N

M

R

Q

E

K

E

G

G

Y

L

A

Y

F
|
F

A
x
T

G

G

A

A

N

N

A

G

Y

Y

L

R

S

V

Q

D

K

K

I

I

V

I

S

S

V

V

R

H

E

E

T

L

I

I

binding S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] octanethioate: L104 (≠ M86), A129 (= A111), G130 (= G112), L131 (= L113), I150 (= I132), I151 (≠ V133), S152 (= S134), E178 (= E160), H185 (= H167), Y280 (≠ M268), I324 (= I308), L328 (= L312)
binding flavin mononucleotide: G25 (= G18), G26 (= G19), M27 (= M20), G28 (= G21), N102 (= N84), E178 (= E160), S182 (≠ A164), G183 (= G165), G223 (= G210), G224 (= G211), M244 (≠ L231), A245 (≠ G232), T246 (≠ S233), L249 (≠ V236), F342 (= F326), T343 (≠ A327)
binding iron/sulfur cluster: C303 (= C291), N306 (≠ H294), C307 (= C295), V308 (≠ I296), C311 (= C299), G314 (vs. gap), A317 (vs. gap), C323 (= C307), I324 (= I308)
binding : L131 (= L113), T133 (≠ L115), N134 (≠ D116), K155 (≠ R137), I159 (≠ L141), K162 (≠ E144), R163 (≠ K145), R167 (≠ N149)

7e1sA Crystal structure of dehydrogenase/isomerase fabx from helicobacter pylori in complex with octanoyl-acp (see paper)
36% identity, 96% coverage: 1:344/359 of query aligns to 8:360/366 of 7e1sA

query
sites
7e1sA

M

L

K

K

S

P

F

L

Y

K

M

I

G

G

N

K

L

H

K

T

I

I

D

K

L

F

P

P

V

I

I

F

Q

Q

G
|
G

G
|
G

M
|
M

G
|
G

V

V

G

G

I

I

S

S

L

W

S

D

G

E

L

L

A

A

S

G

A

N

V

V

A

A

N

K

E

E

G

G

G

A

V

L

G

G

V

V

I

I

S

S

C

A

A

V

G

G

L

T

G

G

L

Y

L

Y

Y

K

P

N

K

M

L

R

S

F

T

V

D

E

Y

R

L

I

-

V

-

A

K

K

N

K

S

P

I

F

L

E

G

A

L

L

R

N

E

F

E

Y

L

S

R

K

K

K

A

A

K

L

E

N

K

E

T

I

-

F

-

A

-

N

-

A

-

R

-

K

-

I

-

C

-

G

N

N

G

N

V

P

I

L

G

G

V

A

N
|
N

I

I

M
x
L

V

Y

A

A

L

I

S

N

N

D

F

Y

S

G

D

R

M

V

V

L

K

R

T

D

A

S

I

C

S

E

E

A

K

G

A

A

D

N

I

I

I

I

F

I

S

T

G

G

A
|
A

G
|
G

L

L

P

P

L

T

D

N

L

M

P

P

S

E

Y

F

L

A

E

K

K

D

E

F

S

S

K

D

T

V

K

A

L

L

V

I

P

P

I
|
I

V
x
I

S
|
S

S

S

S

A

R

K

A

A

A

L

K

K

L

I

I

L

C

C

E

K

K

R

W

W

R

S

N

D

N

R

Y

Y

D

K

Y

R

L

I

P

P

D

D

A

A

I

F

V

I

V

V

E
|
E

G

G

P

P

K

L

A

S

G
|
G

G

G

H
|
H

L

Q

G
|
G

Y

F

K

K

N

Y

D

E

Q

D

I

C

E

F

D

K

E

E

H

E

F

F

S

R

L

L

E

E

H

N

I

L

V

V

P

P

E

K

V

V

I

V

E

E

V

A

I

S

K

K

A

E

Y

W

E

G

K

-

S

-

G

-

H

-

C

-

K

N

I

I

P

P

V

I

I

I

A

A

A

A

G
|
G

G
|
G

L

I

Y

W

T

D

G

R

E

K

D

D

I

I

Y

D

R

T

L

M

M

L

Q

S

L

L

G

G

A

A

T

S

G

G

V

V

Q
|
Q

L
x
M

G
x
A

S
x
T

K

R

F

F

V

L

T

G

T

T

E

K

E

E

C

C

D

D

A

A

S

K

P

-

V

V

F

Y

K

A

Q

D

A

L

Y

L

I

P

D

T

A

L

G

K

Q

K

S

E

D

D

I

I

E

L

I

L

I

I

T

K

S

S

P

P

V

V

G

G

M
x
Y

P

P

G

A

R

R

A

A

I

I

S

N

G

T

E

G

F

V

T

I

R

K

K

R

V

I

K

E

S

E

G

G

S

N

E

A

K

P

P

K

R

I

R

A

C
|
C

A

V

F

S

H
x
N

C
|
C

I
x
V

K

A

T

P

C
|
C

D

N

Y

R

-
x
G

-

E

-

E

-
x
A

T

K

K

K

S

V

P

G

Y

Y

C
|
C

I
|
I

I

A

K

D

A

G

L
|
L

Y

G

N

R

A

S

A

Y

K

L

G

G

N

N

M

R

Q

E

K

E

G

G

Y

L

A

Y

F

F

A

T

G

G

A

A

N

N

A

G

Y

Y

L

R

S

V

Q

D

K

K

I

I

V

I

S

S

V

V

R

H

E

E

T

L

I

I

binding S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] octanethioate: L104 (≠ M86), A129 (= A111), G130 (= G112), I150 (= I132), I151 (≠ V133), S152 (= S134), E178 (= E160), H185 (= H167), G187 (= G169), Y280 (≠ M268), L328 (= L312)
binding flavin mononucleotide: G25 (= G18), G26 (= G19), M27 (= M20), G28 (= G21), N102 (= N84), L104 (≠ M86), E178 (= E160), G183 (= G165), G223 (= G210), G224 (= G211), Q243 (= Q230), M244 (≠ L231), A245 (≠ G232), T246 (≠ S233)
binding iron/sulfur cluster: C303 (= C291), N306 (≠ H294), C307 (= C295), V308 (≠ I296), C311 (= C299), G314 (vs. gap), A317 (vs. gap), C323 (= C307), I324 (= I308)

7e1rA Crystal structure of dehydrogenase/isomerase fabx from helicobacter pylori in complex with holo-acp (see paper)
36% identity, 96% coverage: 1:344/359 of query aligns to 8:360/366 of 7e1rA

query
sites
7e1rA

M

L

K

K

S

P

F

L

Y

K

M

I

G

G

N

K

L

H

K

T

I

I

D

K

L

F

P

P

V

I

I

F

Q

Q

G
|
G

G
|
G

M
|
M

G
|
G

V

V

G

G

I

I

S

S

L

W

S

D

G

E

L

L

A

A

S

G

A

N

V

V

A

A

N

K

E

E

G

G

G

A

V

L

G

G

V

V

I

I

S

S

C

A

A

V

G

G

L

T

G

G

L

Y

L

Y

Y

K

P

N

K

M

L

R

S

F

T

V

D

E

Y

R

L

I

-

V

-

A

K

K

N

K

S

P

I

F

L

E

G

A

L

L

R

N

E

F

E

Y

L

S

R

K

K

K

A

A

K

L

E

N

K

E

T

I

-

F

-

A

-

N

-

A

-

R

-

K

-

I

-

C

-

G

N

N

G

N

V

P

I

L

G

G

V

A

N
|
N

I

I

M
x
L

V

Y

A

A

L

I

S

N

N

D

F

Y

S

G

D

R

M

V

V

L

K

R

T

D

A

S

I

C

S

E

E

A

K

G

A

A

D

N

I

I

I

I

F

I

S

T

G
|
G

A
|
A

G
|
G

L

L

P

P

L

T

D

N

L

M

P

P

S

E

Y

F

L

A

E

K

K

D

E

F

S

S

K

D

T

V

K

A

L

L

V

I

P

P

I

I

V

I

S

S

S

S

S

A

R

K

A

A

A

L

K

K

L

I

I

L

C

C

E

K

K

R

W

W

R

S

N

D

N

R

Y

Y

D

K

Y

R

L

I

P

P

D

D

A

A

I

F

V

I

V

V

E
|
E

G

G

P

P

K

L

A

S

G
|
G

G

G

H
|
H

L

Q

G
|
G

Y

F

K

K

N

Y

D

E

Q

D

I

C

E

F

D

K

E

E

H

E

F

F

S

R

L

L

E

E

H

N

I

L

V

V

P

P

E

K

V

V

I

V

E

E

V

A

I

S

K

K

A

E

Y

W

E

G

K

-

S

-

G

-

H

-

C

-

K

N

I

I

P

P

V

I

I

I

A

A

A

A

G

G

G
|
G

L

I

Y

W

T

D

G

R

E

K

D

D

I

I

Y

D

R

T

L

M

M

L

Q

S

L

L

G

G

A

A

T

S

G

G

V

V

Q
|
Q

L
x
M

G
x
A

S
x
T

K

R

F

F

V

L

T

G

T

T

E

K

E

E

C

C

D

D

A

A

S

K

P

-

V

V

F

Y

K

A

Q

D

A

L

Y

L

I

P

D

T

A

L

G

K

Q

K

S

E

D

D

I

I

E

L

I

L

I

I

T

K

S

S

P

P

V

V

G

G

M

Y

P

P

G

A

R

R

A

A

I

I

S

N

G

T

E

G

F

V

T

I

R

K

K

R

V

I

K

E

S

E

G

G

S

N

E

A

K

P

P

K

R

I

R

A

C
|
C

A

V

F

S

H
x
N

C
|
C

I
x
V

K

A

T

P

C
|
C

D

N

Y

R

-
x
G

-

E

-

E

-
x
A

T

K

K

K

S

V

P

G

Y

Y

C
|
C

I
|
I

I

A

K

D

A

G

L

L

Y

G

N

R

A

S

A

Y

K

L

G

G

N

N

M

R

Q

E

K

E

G

G

Y

L

A

Y

F
|
F

A

T

G

G

A

A

N

N

A

G

Y

Y

L

R

S

V

Q

D

K

K

I

I

V

I

S

S

V

V

R

H

E

E

T

L

I

I

binding flavin mononucleotide: G25 (= G18), G26 (= G19), M27 (= M20), G28 (= G21), N102 (= N84), L104 (≠ M86), E178 (= E160), G183 (= G165), G224 (= G211), Q243 (= Q230), M244 (≠ L231), A245 (≠ G232), T246 (≠ S233), F342 (= F326)
binding N~3~-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-N-(2-sulfanylethyl)-beta-alaninamide: G128 (= G110), A129 (= A111), G130 (= G112), E178 (= E160), H185 (= H167), G187 (= G169)
binding iron/sulfur cluster: C303 (= C291), N306 (≠ H294), C307 (= C295), V308 (≠ I296), C311 (= C299), G314 (vs. gap), A317 (vs. gap), C323 (= C307), I324 (= I308)

7e1qA Crystal structure of dehydrogenase/isomerase fabx from helicobacter pylori (see paper)
36% identity, 96% coverage: 1:344/359 of query aligns to 8:360/366 of 7e1qA

query
sites
7e1qA

M

L

K

K

S

P

F

L

Y

K

M

I

G

G

N

K

L

H

K

T

I

I

D

K

L

F

P

P

V

I

I

F

Q

Q

G
|
G

G
|
G

M
|
M

G
|
G

V

V

G

G

I

I

S

S

L

W

S

D

G

E

L

L

A

A

S

G

A

N

V

V

A

A

N

K

E

E

G

G

G

A

V

L

G

G

V

V

I

I

S

S

C

A

A

V

G

G

L

T

G

G

L

Y

L

Y

Y

K

P

N

K

M

L

R

S

F

T

V

D

E

Y

R

L

I

-

V

-

A

K

K

N

K

S

P

I

F

L

E

G

A

L

L

R

N

E

F

E

Y

L

S

R

K

K

K

A

A

K

L

E

N

K

E

T

I

-

F

-

A

-

N

-

A

-

R

-

K

-

I

-

C

-

G

N

N

G

N

V

P

I

L

G

G

V

A

N
|
N

I

I

M
x
L

V

Y

A

A

L

I

S

N

N

D

F

Y

S

G

D

R

M

V

V

L

K

R

T

D

A

S

I

C

S

E

E

A

K

G

A

A

D

N

I

I

I

I

F

I

S

T

G

G

A

A

G

G

L

L

P

P

L

T

D

N

L

M

P

P

S

E

Y

F

L

A

E

K

K

D

E

F

S

S

K

D

T

V

K

A

L

L

V

I

P

P

I

I

V

I

S

S

S

S

S

A

R

K

A

A

A

L

K

K

L

I

I

L

C

C

E

K

K

R

W

W

R

S

N

D

N

R

Y

Y

D

K

Y

R

L

I

P

P

D

D

A

A

I

F

V

I

V

V

E
|
E

G

G

P

P

K

L

A
x
S

G
|
G

G

G

H

H

L

Q

G

G

Y

F

K

K

N

Y

D

E

Q

D

I

C

E

F

D

K

E

E

H

E

F

F

S

R

L

L

E

E

H

N

I

L

V

V

P

P

E

K

V

V

I

V

E

E

V

A

I

S

K

K

A

E

Y

W

E

G

K

-

S

-

G

-

H

-

C

-

K

N

I

I

P

P

V

I

I

I

A

A

A

A

G
|
G

G
|
G

L

I

Y

W

T

D

G

R

E

K

D

D

I

I

Y

D

R

T

L

M

M

L

Q

S

L

L

G

G

A

A

T

S

G

G

V

V

Q

Q

L
x
M

G
x
A

S
x
T

K

R

F

F

V

L

T

G

T

T

E

K

E

E

C

C

D

D

A

A

S

K

P

-

V

V

F

Y

K

A

Q

D

A

L

Y

L

I

P

D

T

A

L

G

K

Q

K

S

E

D

D

I

I

E

L

I

L

I

I

T

K

S

S

P
|
P

V

V

G

G

M

Y

P

P

G

A

R

R

A

A

I

I

S

N

G

T

E

G

F

V

T

I

R

K

K

R

V

I

K

E

S

E

G

G

S

N

E

A

K

P

P

K

R

I

R

A

C
|
C

A

V

F

S

H
x
N

C
|
C

I
x
V

K

A

T

P

C
|
C

D

N

Y

R

-
x
G

-

E

-

E

-
x
A

T

K

K

K

S

V

P

G

Y

Y

C
|
C

I
|
I

I

A

K

D

A

G

L

L

Y

G

N

R

A

S

A

Y

K

L

G

G

N

N

M

R

Q

E

K

E

G

G

Y

L

A

Y

F
|
F

A

T

G

G

A

A

N

N

A

G

Y

Y

L

R

S

V

Q

D

K

K

I

I

V

I

S

S

V

V

R

H

E

E

T

L

I

I

binding flavin mononucleotide: G25 (= G18), G26 (= G19), M27 (= M20), G28 (= G21), N102 (= N84), L104 (≠ M86), E178 (= E160), S182 (≠ A164), G183 (= G165), G223 (= G210), G224 (= G211), M244 (≠ L231), A245 (≠ G232), T246 (≠ S233), F342 (= F326)
binding iron/sulfur cluster: P277 (= P265), C303 (= C291), N306 (≠ H294), C307 (= C295), V308 (≠ I296), C311 (= C299), G314 (vs. gap), A317 (vs. gap), C323 (= C307), I324 (= I308)

5gvhA Structure of fabk from thermotoga maritima (see paper)
29% identity, 98% coverage: 9:359/359 of query aligns to 10:308/311 of 5gvhA

query
sites
5gvhA

L

L

K

E

I

I

D

E

L

H

P

P

V

I

I

L

Q

M

G
|
G

G
|
G

M
|
M

G
x
A

V

W

G

A

I

-

S

G

L

T

S

P

G

T

L

L

A

A

S

A

A

A

V

V

A

S

N

E

E

A

G

G

G

G

V

L

G

G

V

I

I

I

S

-

C

-

A

-

G

G

L

S

G

G

L

A

L

M

Y

K

P

P

K

-

L

-

S

-

T

-

D

D

Y

D

L

L

K

R

N

K

S

A

I

I

L

S

G

E

L

L

R

R

E

Q

E

-

L

-

R

-

K

-

A

-

K

-

E

-

K

K

T

T

N

D

G

K

V

P

I

F

G

G

V

V

N
|
N

I

I

M
x
I

V

L

A

V

L

S

S

P

N

W

F

A

S

D

D

D

M

L

V

V

K

K

T

V

A

C

I

I

S

E

E

E

K

K

A

V

D

P

I

V

I

V

F

T

S

F

G

G

A

A

G

G

L

N

P

P

L

T

D

K

L

Y

P

I

S

R

Y

E

L

L

E

-

K

K

E

E

S

N

K

G

T

T

K

K

L

V

V

I

P

P

I

V

V

V

S

A

S

S

S

D

R

S

A

L

A

A

K

R

L

M

I

V

C

E

E

R

K

A

W

G

R

-

N

-

N

-

Y

-

D

-

Y

-

L

-

P

A

D

D

A

A

I

V

V

I

V

A

E
|
E

G

G

P

M

K

E

A
x
S

G
|
G

G

G

H

H

L

I

G

G

Y

-

K

-

N

-

D

-

Q

-

I

-

E

-

D

-

E

-

H

-

F

-

S

-

L

-

E

-

H

-

I

-

V

-

P

-

E

E

V

V

I

T

E

T

V

F

I

V

K

L

A

V

Y

N

E

K

K

V

S

S

G

R

H

S

C

V

K

N

I

I

P

P

V

V

I

I

A

A

A

A

G
|
G

G
|
G

L

I

Y

A

T

D

G

G

E

R

D

G

I

M

Y

A

R

A

L

A

M

F

Q

A

L

L

G

G

A

A

T

E

G

A

V

V

Q
|
Q

L

M

G
|
G

S
x
T

K

R

F

F

V

V

T

A

T

S

E

V

E

E

C

S

D

D

A

V

S

H

P

P

V

V

F

Y

K

K

Q

E

A

K

Y

I

I

V

D

K

A

A

G

S

Q

I

S

R

D

D

I

T

E

V

I

V

I

T

T

G

S

A

P

K

V

L

G

G

M

H

P

P

G

A

R

R

A

V

I

L

S

R

G

T

E

P

F

F

T

A

R

R

K

K

V

I

K

Q

S

E

G

-

S

-

E

-

K

-

P

-

R

-

R

-

C

-

A

-

F

-

H

-

C

-

I

M

K

E

T

F

C

E

D

N

Y

P

T

M

K

Q

S

A

P

E

Y

E

C

M

I

L

I

V

K

G

A

S

L

L

Y

R

N

R

A

A

-

V

A

V

K

E

G

G

N

D

M

L

Q

E

K

R

G

G

Y

S

A

F

F
x
M

A

V

G

G

A

Q

N
x
S

A

A

Y

G

L

L

S

I

Q

D

K

E

I

I

V

K

S

P

V

V

R

K

E

Q

T

I

I

I

D

E

I

D

L

I

K

L

K

K

E

E

F

F

M

K

Q

E

A

T

K

V

E

E

S

K

V

L

K

R

binding flavin mononucleotide: G19 (= G18), G20 (= G19), M21 (= M20), A22 (≠ G21), N70 (= N84), I72 (≠ M86), E138 (= E160), S142 (≠ A164), G143 (= G165), G170 (= G210), G171 (= G211), Q190 (= Q230), G192 (= G232), T193 (≠ S233), M275 (≠ F326), S279 (≠ N330)

7l00C Crystal structure of c. Difficile enoyl-acyl carrier protein reductase (fabk) in complex with an inhibitor
28% identity, 97% coverage: 9:356/359 of query aligns to 14:314/315 of 7l00C

query
sites
7l00C

L

L

K

N

I

I

D

K

L

Y

P

P

V

V

I

I

Q

Q

G
|
G

G
|
G

M
|
M

G
x
A

V

-

G

W

I

V

S

A

L

T

S

A

G

S

L

L

A

A

S

S

A

A

V

V

A

S

N

N

E

A

G

G

G

G

V

L

G

G

V

I

I

I

S

A

C

A

A

G

G

N

L

-

G

-

L

-

L

-

Y

A

P

P

K

K

L

E

S

A

T

-

D

-

Y

-

L

-

K

-

N

-

S

-

I

-

L

-

G

-

L

I

R

K

E

K

E

E

L

I

R

V

K

E

A

C

K

K

E

K

K

L

T

T

N

D

G

K

V

P

I

F

G

G

V

V

N
|
N

I

V

M
|
M

V

L

A

M

L

S

S

P

N

F

F

V

S

D

D

D

M

I

V

I

K

D

T

L

A

I

I

I

S

E

E

E

K

K

A

V

D

Q

I

V

I

I

F

T

S

T

G
|
G

A
|
A

G
|
G

L

N

P

P

L

A

D

K

L

Y

P

M

S

D

Y

R

L

L

E

-

K

K

E

E

S

A

K

G

T

T

K

K

L

V

V

I

P

P

I
x
V

V

V

S
x
P

S

T

S

I

R

A

A

L

A

A

K

Q

L

R

I

M

C

-

E

E

K

K

W

L

R

G

N

A

N

T

Y

-

D

-

Y

-

L

-

P

-

D

-

A

A

I

V

V

I

V

A

E
|
E

G

G

P

T

K

E

A
x
G

G
|
G

G

G

H
|
H

L

I

G

G

Y

E

K

L

N

T

D

T

Q

M

I

V

E

-

D

-

E

-

H

-

F

-

S

-

L

-

E

-

H

-

I

L

V

V

P

P

E

Q

V

V

I

A

E

D

V

A

I

V

K

N

A

-

Y

-

E

-

K

-

S

-

G

-

H

-

C

-

K

-

I

I

P

P

V

V

I

I

A

A

A

A

G
|
G

G
|
G

L

I

Y

V

T

D

G

G

E

R

D

G

I

I

Y

A

R

A

L

S

M

F

Q

A

L

L

G

G

A

A

T

S

G

A

V

V

Q
|
Q

L

V

G
|
G

S
x
T

K

R

F

F

V

I

T

C

T

S

E

E

E

E

C

C

D

S

A

V

S

H

P

S

V

N

F

Y

K

K

Q

N

A

L

Y

V

I

L

D

K

A

A

G

K

Q

D

S

R

D

D

I

A

E

I

I

V

I

T

T

G

S

R

P

S

V

T

G

G

M

H

P

P

G

V

R

R

A

T

I

L

S

K

G

N

E

K

F

L

T

S

R

K

-

E

-

F

-

L

K

K

V

M

K

E

S

Q

G

N

S

G

E

A

K

T

P

P

R

E

R

-

C

-

A

-

F

-

H

-

C

-

I

-

K

-

T

-

C

-

D

E

Y

L

T

D

K

K

S

K

P

G

Y

T

C

G

I

A

I
x
L

K

R

A

-

L

-

Y

F

N

A

A

T

A

V

K

D

G

G

N

D

M

I

Q

E

K

K

G

G

Y

S

A

F

F
x
M

A

A

G

G

A

Q

N
x
S

A

A

Y

A

L

M

S

V

Q

K

K

E

I

I

V

T

S

P

V

C

R

K

E

E

T

I

I

I

D

E

I

A

L

M

-

V

-

N

-

Q

K

A

K

R

E

E

F

I

M

M

Q

P

A

A

K

I

E

E

binding flavin mononucleotide: G23 (= G18), G24 (= G19), M25 (= M20), A26 (≠ G21), N74 (= N84), E142 (= E160), G146 (≠ A164), G147 (= G165), G174 (= G210), G175 (= G211), Q194 (= Q230), G196 (= G232), T197 (≠ S233), M281 (≠ F326), S285 (≠ N330)
binding N-[6-(methylsulfonyl)-1,3-benzothiazol-2-yl]-2-[2-(1H-pyrrol-1-yl)-1,3-thiazol-4-yl]acetamide: A26 (≠ G21), M76 (= M86), G100 (= G110), A101 (= A111), G102 (= G112), V121 (≠ I132), P123 (≠ S134), H149 (= H167), L266 (≠ I309), M281 (≠ F326)

2z6iB Crystal structure of s. Pneumoniae enoyl-acyl carrier protein reductase (fabk) (see paper)
33% identity, 78% coverage: 1:279/359 of query aligns to 1:238/321 of 2z6iB

query
sites
2z6iB

M

M

K

K

S

T

F

R

Y

I

M

T

G

E

N

L

L

L

K

K

I

I

D

D

L

Y

P

P

V

I

I

F

Q

Q

G
|
G

G
|
G

M
|
M

G
x
A

V

-

G

W

I

V

S

A

L

D

S

G

G

D

L

L

A

A

S

G

A

A

V

V

A

S

N

K

E

A

G

G

G

G

V

L

G

G

V

I

I

I

S

G

C

G

A

G

G

N

L

-

G

-

L

-

L

-

Y

A

P

P

K

K

L

-

S

-

T

-

D

E

Y

V

L

V

K

K

N

A

S

N

I

I

L

-

G

-

L

-

R

-

E

-

E

-

L

-

R

D

K

K

A

I

K

K

E

S

K

L

T

T

N

D

G

K

V

P

I

F

G

G

V

V

N
|
N

I

I

M

M

V

L

A

L

L

S

S

P

N

F

F

V

S

E

D

D

M

I

V

V

K

D

T

L

A

V

I

I

S

E

E

E

K

G

A

V

D

K

I

V

I

V

F

T

S

T

G

G

A

A

G

G

L

N

P

P

L

-

D

-

L

-

P

S

S

K

Y

Y

L

M

E

E

K

R

-

F

-

H

E

E

S

A

K

G

T

I

K

I

L

V

V

I

P

P

I

V

V

V

S

P

S

S

S

V

R

A

A

L

A

A

K

K

L

R

I

M

C

-

E

E

K

K

W

-

R

-

N

-

N

-

Y

-

D

-

Y

I

L

G

P

A

D

D

A

A

I

V

V

I

V

A

E
|
E

G

G

P

M

K

E

A
|
A

G
|
G

G

G

H
|
H

L

I

G

G

Y

-

K

-

N

-

D

-

Q

-

I

-

E

-

D

-

E

-

H

K

F

L

S

T

L

T

E

M

H

T

I

L

V

V

P

R

E

Q

V

V

I
x
A

E

T

V

A

I
|
I

K

S

A

-

Y

-

E

-

K

-

S

-

G

-

H

-

C

-

K

-

I

I

P

P

V

V

I

I

A

A

A

A

G
|
G

G
|
G

L

I

Y

A

T

D

G

G

E

E

D

G

I

A

Y

A

R

A

L

G

M

F

Q

M

L

L

G

G

A

A

T

E

G

A

V

V

Q

Q

L

V

G
|
G

S
x
T

K

R

F

F

V

V

T

V

T

A

E

K

E

E

C

S

D

N

A

A

S

H

P

P

V

N

F

Y

K

K

Q

E

A

K

Y

I

I

L

D

K

A

A

G

R

Q

D

S

I

D

D

I

T

E

T

I

I

I

S

T

A

S

Q

P

H

V

F

G

G

M

H

P

A

G

V

R

R

A

A

I

I

S

K

G

N

E

Q

F

L

T

T

R

R

binding calcium ion: E137 (= E160), H144 (= H167), A158 (≠ I190), I161 (= I193)
binding flavin mononucleotide: G18 (= G18), G19 (= G19), M20 (= M20), A21 (≠ G21), N69 (= N84), E137 (= E160), A141 (= A164), G142 (= G165), G169 (= G210), G170 (= G211), G191 (= G232), T192 (≠ S233)

Sites not aligning to the query:

binding flavin mononucleotide: 281

4iqlA Crystal structure of porphyromonas gingivalis enoyl-acp reductase ii (fabk) with cofactors NADPH and fmn (see paper)
30% identity, 78% coverage: 9:287/359 of query aligns to 8:246/313 of 4iqlA

query
sites
4iqlA

L

L

K

G

I

I

D

E

L

H

P

P

V

I

I

I

Q

S

G

G

G
|
G

M
|
M

G

-

V
|
V

G
x
W

I

C

S

S

L

G

S
x
W

G

K

L

L

A

A

S

S

A

A

V

V

A

S

N

N

E

C

G

G

G

G

V

L

G

G

V

L

I

I

S

-

C

-

A

-

G

G

L

A

G

G

L
x
S

L
x
M

Y
x
H

P

P

K

-

L

-

S

-

T

-

D

D

Y
x
N

L

L

K

E

N
x
H

S
x
H

I

I

L

-

G

-

L

-

R

-

E

-

E

-

L

-

R

R

K

S

A

C

K

K

E

A

K

A

T

T

N

D

G

K

V

P

I

F

G

G

V

V

N
|
N

I

V

M

P

V

L

A

L

L
x
Y

S
x
P

N
x
E

F

M

S

D

D

K

M

I

V

M

K

E

T

I

A

I

I

M

S

R

E

E

K

H

A

V

D

P

I

V

I

V

F

V

S

T

G
x
S

A

A

G

G

L

S

P

P

L

K

D

V

L

W

P

T

S

A

Y

K

L

L

E

-

K

K

E

A

S

A

K

G

T

S

K

K

L

V

V

I

P

H

I

V

V

V

S

S

S

S

S

A

R

T

A

F

A

A

K

-

L

-

I

-

C

-

E

-

K

-

W

-

R

R

N

K

N

S

Y

E

D

A

Y

A

L

G

P

V

D

D

A

A

I

I

V

V

V

A

E
|
E

G

G

P

F

K

E

A
|
A

G
|
G

G

G

H

H

L

N

G

G

Y

R

K

-

N

-

D

-

Q

-

I

-

E

-

D

-

E

E

H

E

F

T

S

T

L

T

E

L

H

C

I

L

V

I

P

P

E

E

V

V

I

V

E

D

V

A

I

V

K

N

A

-

Y

-

E

-

K

-

S

-

G

-

H

-

C

-

K

-

I

I

P

P

V

V

I

V

A

A

A

A

G
|
G

G
|
G

L

I

Y

A

T

S

G

G

E

R

D

A

I

V

Y

A

R

A

L

A

M

L

Q

A

L

L

G

G

A

A

T

D

G

A

V

V

Q
|
Q

L

V

G
|
G

S
x
T

K

R

F

F

V

A

T

L

T

S

E

E

E

E

C

S

D
x
S

A

A

S

H

P

E

V

D

F

F

K

K

Q

A

A

H

Y

C

I

R

D

R

A

S

G

V

Q

E

S

G

D

D

I

T

E

M

I

L

I

S

T

L

S

K

P

A

V

V

G

S

M

-

P

P

G

T

R

R

A

L

I

L

S

K

G

N

E

K

F

F

T

Y

R

Q

K

D

V

V

K

F

S

A

G

A

S

E

E

Q

K

R

binding flavin mononucleotide: G18 (= G19), M19 (= M20), V20 (= V22), N68 (= N84), S94 (≠ G110), E136 (= E160), A140 (= A164), G141 (= G165), G170 (= G210), G171 (= G211), Q190 (= Q230), G192 (= G232), T193 (≠ S233)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: W21 (≠ G23), W21 (≠ G23), W25 (≠ S27), S44 (≠ L49), M45 (≠ L50), M45 (≠ L50), H46 (≠ Y51), N49 (≠ Y58), H52 (≠ N61), H53 (≠ S62), Y73 (≠ L89), P74 (≠ S90), E75 (≠ N91), S202 (≠ D242)

Sites not aligning to the query:

2z6jA Crystal structure of s. Pneumoniae enoyl-acyl carrier protein reductase (fabk) in complex with an inhibitor (see paper)
32% identity, 78% coverage: 1:279/359 of query aligns to 1:235/307 of 2z6jA

query
sites
2z6jA

M

M

K

K

S

T

F

R

Y

I

M

T

G

E

N

L

L

L

K

K

I

I

D

D

L

Y

P

P

V

I

I

F

Q

Q

G
|
G

G
|
G

M
|
M

G
x
A

V

-

G

W

I

V

S

A

L

D

S

G

G

D

L

L

A

A

S

G

A

A

V

V

A

S

N

K

E

A

G

G

G

G

V

L

G

G

V

I

I

I

S

G

C

G

A

G

G
x
N

L

-

G

-

L

-

L

-

Y

A

P

P

K

K

L

-

S

-

T

-

D

E

Y

V

L

V

K

K

N

A

S

N

I

I

L

-

G

-

L

-

R

-

E

-

E

-

L

-

R

D

K

K

A

I

K

K

E

S

K

L

T

T

N

D

G

K

V

P

I

F

G

G

V

V

N
|
N

I

I

M
|
M

V

L

A
x
L

L

S

S

P

N

F

F

V

S

E

D

D

M

I

V

V

K

D

T

L

A

V

I

I

S

E

E

E

K

G

A

V

D

K

I

V

I

V

F

T

S

T

G

G

A
|
A

G
|
G

L

N

P

P

L

-

D

-

L

-

P

S

S

K

Y

Y

L

M

E

E

K

R

-

F

-

H

E

E

S

A

K

G

T

I

K

I

L

V

V

I

P

P

I
x
V

V

V

S
x
P

S

S

S

V

R

A

A
x
L

A

A

K

K

L

R

I

M

C

-

E

E

K

K

W

-

R

-

N

-

N

-

Y

-

D

-

Y

I

L

G

P

A

D

D

A

A

I

V

V

I

V

A

E
|
E

G

G

P

M

K

E

A
|
A

G
|
G

G

G

H
|
H

L

I

G

G

Y

-

K

-

N

-

D

-

Q

-

I

-

E

-

D

-

E

-

H

K

F

L

S

T

L

T

E

M

H

T

I

L

V

V

P

R

E

Q

V

V

I
x
A

E

T

V

A

I
|
I

K

S

A

-

Y

-

E

-

K

-

S

-

G

-

H

-

C

-

K

-

I

I

P

P

V

V

I

I

A

A

A

A

G
|
G

G
|
G

L

I

Y

A

T

D

G

G

E

E

D

G

I

A

Y

A

R

A

L

G

M

F

Q

M

L

L

G

G

A

A

T

E

G

A

V

V

Q

Q

L

V

G
|
G

S
x
T

K

R

F

F

V

V

T

V

T

A

E

K

E

E

C

S

D

N

A

A

S

H

P

P

V

N

F

Y

K

K

Q

E

A

K

Y

I

I

L

D

K

A

A

G

R

Q

D

S

I

D

D

I

T

E

T

I

I

I

S

T

A

S

Q

P

-

V

-

G

-

M

H

P

A

G

V

R

R

A

A

I

I

S

K

G

N

E

Q

F

L

T

T

R

R

binding calcium ion: A158 (≠ I190), I161 (= I193)
binding flavin mononucleotide: G18 (= G18), G19 (= G19), M20 (= M20), A21 (≠ G21), N69 (= N84), E137 (= E160), A141 (= A164), G142 (= G165), G169 (= G210), G170 (= G211), G191 (= G232), T192 (≠ S233)
binding 2-(4-(2-((3-(5-(pyridin-2-ylthio)thiazol-2-yl)ureido)methyl)-1h-imidazol-4-yl)phenoxy)acetic acid: N45 (≠ G46), M71 (= M86), L73 (≠ A88), A96 (= A111), G97 (= G112), V116 (≠ I132), P118 (≠ S134), L122 (≠ A138), E137 (= E160), H144 (= H167)

5lsmA Crystal structure of nitronate monooxygenase (so_0471) from shewanella oneidensis mr-1
26% identity, 74% coverage: 11:277/359 of query aligns to 9:274/339 of 5lsmA

query
sites
5lsmA

I

I

D

E

L

F

P

P

V

I

I

I

Q

Q

G
x
A

G
x
P

M
|
M

G
x
A

V

-

G

G

I

V

S

Q

L

G

S

S

G

A

L

L

A

A

S

I

A

A

V

V

A

S

N

E

E

A

G

G

G

G

V

L

G

G

V

S

I

L

S

P

C

C

A

A

G

M

L

L

G

-

L

-

L

-

Y

-

P

-

K

-

L

-

S

-

T

-

D

-

Y

-

L

-

K

-

N

-

S

S

I

L

L

E

G

A

L

L

R

E

E

A

E

E

L

L

R

T

K

A

A

I

K

R

E

S

K

Q

T

T

N

A

G

K

V

P

I

I

G

N

V

V

N
|
N

I

F

M

F

V

C

A

H

L

R

S

E

N

P

F

V

S

A

D

Q

M

A

V

A

K

K

T

Q

A

A

I

A

S

W

-

L

E

E

K

Q

A

L

D

A

I

P

I

Y

F

F

S

A

G

E

A

F

G

N

L

L

-

D

P

P

L

N

D

R

L

T

P

P

-

Y

S

S

Y

K

L

A

E

Q

K

A

E

E

S

V

K

L

T

A

K

K

L

F

V

K

P

P

I

E

V

V

S

V

S

S

-

F

-
x
H

-

F

-

G

-

L

-

P

-

D

-

E

-

E

-

L

-

L

-

L

-

E

-

I

-

K

S

S

R

W

A

G

A

S

K

K

L

V

I

I

C

S

E

T

K

A

-

T

-

T

-

V

-

E

-

E

-

A

-

L

W

W

R

L

N

E

N

A

Y

R

D

G

Y

A

L

-

P

-

D

D

A

A

I

I

V

I

V

A

E

Q

G

G

P

L

K

E

A
|
A

G
|
G

G

G

H

H

L

R

G

G

Y

H

K

F

N

L

D

S

Q

E

I

D

E

L

D

T

E

E

H

Q

F

L

S

G

L

T

E

F

H

S

I

L

V

L

P

P

E

Q

V

I

I

I

E

A

V

A

I

V

K

E

A

-

Y

-

E

-

K

-

S

-

G

-

H

-

C

-

K

-

I

I

P

P

V

V

I

I

A

A

A

A

G
|
G

G
|
G

L

I

Y

V

T

D

G

A

E

T

D

T

I

V

Y

R

R

A

L

A

M

M

Q

T

L

M

G

G

A

A

T

S

G

A

V

V

Q

Q

L

V

G
|
G

S
x
T

K

A

F

Y

V

L

T

L

T

C

E

P

E

E

C

C

D

N

A

T

S

S

P

A

V

I

F

H

K

R

Q

E

A

A

Y

L

I

Q

D

S

A

D

G

A

Q

A

S

Q

D

H

I

T

E

A

I

L

I

T

T

N

S

L

P

F

V

S

G

G

M

R

P

P

G

A

R

R

A

G

I

I

S

V

G

N

E

R

F

F

binding flavin mononucleotide: A16 (≠ G18), P17 (≠ G19), M18 (= M20), A19 (≠ G21), N67 (= N84), H123 (vs. gap), A170 (= A164), G171 (= G165), G207 (= G210), G208 (= G211), G229 (= G232), T230 (≠ S233)

Sites not aligning to the query:

binding flavin mononucleotide: 314

6bkaA Crystal structure of nitronate monooxygenase from cyberlindnera saturnus (see paper)
26% identity, 97% coverage: 5:351/359 of query aligns to 8:374/374 of 6bkaA

query
sites
6bkaA

Y

F

M

L

G

K

N

T

L

F

K

E

I

V

D

R

L

Y

P

P

V

I

I

I

Q

Q

G
x
A

G
x
P

M
|
M

G
x
A

V

-

G

G

I

A

S

S

L

T

S

L

G

E

L

L

A

A

S

A

A
x
T

V

V

A

T

N
x
R

E

L

G

G

G

G

V

I

G

G

V

S

I

I

S

P

C

M

A

G

G

S

L

L

G

S

L

E

L

K

Y

C

P

D

K

A

L

I

S

E

T

T

D

Q

Y

-

L

L

K

E

N

N

-

F

-

D

S

E

I

L

L
x
V

G

G

-
x
D

-

S

-

G

-

R

-

I

L

V

R

N

E

L

E
x
N

L

F

R
x
F

K

A

A

H

K

K

E

E

K

P

T

R

N

S

G

G

V

R

I

A

G

D

V

V

N

N

I

-

M

-

V

-

A

-

L

-

S

-

N

-

F

-

S

E

D

E

M

W

V

L

K

K

-

K

-

Y

T

D

A

K

I

I

S

Y

E

G

K

K

A

A

D

G

I

I

I

E

F

F

S

D

G

K

A

K

G

E

L

L

P

K

L

L

D

L

L

Y

P

P

S

S

Y

F

-

R

-

S

-

I

-

V

-

D

-

P

-

Q

-

H

-

P

-

T

-

V

-

R

L

L

E

L

K

K

E

N

S

L

K

K

T

P

K

K

L

I

V

V

P

S

-

F

-
x
H

-

F

-

G

-

L

-

P

-

H

-

E

-

A

I

V

V

I

S

E

S

S

S

L

R

Q

A

A

A

S

K

D

L

I

I

K

-

I

-

F

-

V

-

T

-

V

-

T

-

N

C

L

E

Q

K

E

W

F

R

Q

N

Q

N

A

Y

Y

D

E

Y

S

L

K

P

L

D

D

A

G

I

V

V

V

V

L

E
x
Q

G

G

P

W

K

E

A
|
A

G
|
G

G
|
G

H

H

L

R

G

G

-

N

Y

F

K

K

N

A

D

N

Q

D

I

V

E

E

D

D

E

G

H

Q

F

L

S

K

L

T

E

L

H

D

I

L

V

V

P

S

E

T

V

I

I

V

E

D

V

Y

I

I

K

D

A

S

Y

A

E

S

K

I

S

S

G

N

H

P

C

P

K

F

I

I

P

-

V

-

I

I

A

A

A

A

G
|
G

G
|
G

L

I

Y

H

T

D

G

D

E

E

D

S

I

I

Y

K

R

E

L

L

M

L

Q

Q

L

F

G

N

A

I

T

A

G

A

V

V

Q

Q

L

L

G
|
G

S
x
T

K

V

F

W

V

L

T

P

T

S

E

S

E

Q

C

A

D

T

A

I

S

S

P

P

V

E

F

H

K

L

Q

K

A

M

Y

F

I

-

D

Q

A

S

G

P

Q

K

S

S

D

D

I

T

E

M

I

M

I

T

T

A

S

A

P

I

V

S

G

G

M

R

P

N

G

L

R

R

A

T

I

I

S

S

G

T

E

P

F

F

T

L

R

R

K

D

V

L

K

H

S

Q

G

S

S

S

E

P

K

-

P

-

R

-

R

-

C

-

A

-

F

-

H

-

C

L

I

A

K

S

T

I

C

P

D

D

Y

Y

T

P

K

L

S

-

P

P

Y

Y

C

D

I

S

I

F

K

K

A

S

L

L

Y

A

N

N

A

D

A

A

K

K

-

Q

-

S

G

G

N

K

M

G

Q

P

K

Q

G

Y

Y

S

A

A

F

F

-

L

A

A

G

G

A

S

N

N

A

Y

Y

H

L

K

S

S

Q

W

K

K

I

D

V

T

-

R

S

S

V

T

R

E

E

E

T

I

I

F

D

S

I

I

L

L

K

V

K

Q

E

D

F

L

binding 3,6,9,12,15,18,21,24,27,30,33,36-dodecaoxaoctatriacontane-1,38-diol: T34 (≠ A32), R37 (≠ N35), V67 (≠ L64), D69 (vs. gap)
binding flavin mononucleotide: A21 (≠ G18), P22 (≠ G19), M23 (= M20), A24 (≠ G21), N77 (≠ E69), F79 (≠ R71), H147 (vs. gap), Q190 (≠ E160), A194 (= A164), G195 (= G165), G196 (= G166), G239 (= G210), G240 (= G211), G261 (= G232), T262 (≠ S233)

4q4kA Crystal structure of nitronate monooxygenase from pseudomonas aeruginosa pao1 (see paper)
25% identity, 70% coverage: 1:250/359 of query aligns to 1:257/351 of 4q4kA

query
sites
4q4kA

M

M

K

T

S

D

F

R

Y

F

M

T

G

R

N

L

L

L

K

G

I

I

D

Q

L

Q

P

P

V

I

I

I

Q

Q

G
x
A

G
x
P

M
|
M

G

-

V
x
L

G

G

I

V

S

S

L

T

S

P

G

A

L

L

A

A

S

A

A

A

V

V

A

S

N

N

E

A

G

G

G

G

V

L

G

G

V

S

I

I

S

A

C

I

A

T

G

G

L

-

G

-

L

-

L

-

Y

-

P

-

K

-

L

-

S

-

T

-

D

-

Y

-

L

-

K

-

N

S

S

A

I

A

L

E

G

K

L

G

R

R

E

A

E

L

L

I

R

R

K

E

A

V

K

R

E

G

K

L

T

T

N

D

G

K

V

P

I

F

G

N

V

V

N
|
N

I

L

M
x
F

V

C

A

H

L

R

S

P

N

G

F

Q

S

A

D

D

M

P

V

A

K

R

-

E

T

R

A

A

I

W

S

L

E

D

K

Y

A

L

D

K

I

P

I

L

F

F

S

A

-

E

-

F

G

G

A

A

G

E

L

P

P

P

L

V

D

R

L

L

P

K

S

N

-

I

Y

Y

L

L

E

S

K

F

E

L

S

E

K

D

T

P

K

T

L

L

V

L

P

P

I

M

V

L

S

L

S

E

S

E

R

R

A

P

A

A

K

A

-

V

-

S

-

F

-
x
H

-

F

-

G

-

A

-

P

-

P

-

R

-

D

-

Q

-

V

-

R

-

A

-

L

-

Q

-

A

-

V

-

G

-

I

-

R

-

V

L

L

I

V

C

C

E

A

K

T

W

T

R

P

N

E

N

E

Y

A

D

A

Y

L

L

V

-

E

-

A

-

A

-

G

P

A

D

D

A

A

I

V

V

V

V

A

E
x
Q

G

G

P

I

K

E

A
|
A

G
|
G

G
|
G

H

H

L

R

G

G

Y

V

K

F

N

E

D

P

Q

E

I

R

E

G

D

D

E

A

H

A

F

I

S

G

L

-

E

-

H

-

I

-

V

-

P

-

E

-

V

T

I

L

E

A

V

L

I

V

K

R

A

L

Y

L

E

A

K

A

S

R

G

G

H

-

C

-

K

S

I

L

P

P

V

V

I

V

A

A

A

A

G
|
G

G
|
G

L

I

Y

M

T

D

G

G

E

R

D

G

I

I

Y

R

R

A

L

A

M

L

Q

E

L

L

G

G

A

A

T

S

G

A

V

V

Q

Q

L

M

G
|
G

S
x
T

K

A

F

F

V

V

T

L

T

C

E

P

E

E

C

S

D

S

A

A

S

N

P

A

V

A

F

Y

K

R

Q

E

A

A

binding flavin mononucleotide: A18 (≠ G18), P19 (≠ G19), M20 (= M20), L21 (≠ V22), N69 (= N84), F71 (≠ M86), H133 (vs. gap), Q176 (≠ E160), A180 (= A164), G181 (= G165), G182 (= G166), G217 (= G210), G218 (= G211), G239 (= G232), T240 (≠ S233)

Sites not aligning to the query:

binding flavin mononucleotide: 325

Q9HWH9 Nitronate monooxygenase; NMO; Propionate 3-nitronate monooxygenase; P3N monooxygenase; EC 1.13.12.- from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
25% identity, 70% coverage: 1:250/359 of query aligns to 1:257/351 of Q9HWH9

query
sites
Q9HWH9

M

M

K

T

S

D

F

R

Y

F

M

T

G

R

N

L

L

L

K

G

I

I

D

Q

L

Q

P

P

V

I

I

I

Q

Q

G

A

G

P

M

M

G

-

V
x
L

G

G

I

V

S

S

L

T

S

P

G

A

L

L

A

A

S

A

A

A

V

V

A

S

N

N

E

A

G

G

G

G

V

L

G

G

V

S

I

I

S

A

C

I

A

T

G

G

L

-

G

-

L

-

L

-

Y

-

P

-

K

-

L

-

S

-

T

-

D

-

Y

-

L

-

K

-

N

S

S

A

I

A

L

E

G

K

L

G

R

R

E

A

E

L

L

I

R

R

K

E

A

V

K

R

E

G

K

L

T

T

N

D

G

K

V

P

I

F

G

N

V

V

N
|
N

I

L

M

F

V

C

A

H

L

R

S

P

N

G

F

Q

S

A

D

D

M

P

V

A

K

R

-

E

T

R

A

A

I

W

S

L

E

D

K

Y

A

L

D

K

I

P

I

L

F

F

S

A

-

E

-

F

G

G

A

A

G

E

L

P

P

P

L

V

D

R

L

L

P

K

S

N

-

I

Y

Y

L

L

E

S

K

F

E

L

S

E

K

D

T

P

K

T

L

L

V

L

P

P

I

M

V

L

S

L

S

E

S

E

R

R

A

P

A

A

K

A

-

V

-

S

-

F

-

H

-

F

-

G

-

A

-

P

-

P

-

R

-

D

-

Q

-

V

-

R

-

A

-

L

-

Q

-

A

-

V

-

G

-

I

-

R

-

V

L

L

I

V

C

C

E

A

K

T

W

T

R

P

N

E

N

E

Y

A

D

A

Y

L

L

V

-

E

-

A

-

A

-

G

P

A

D

D

A

A

I

V

V

V

V

A

E
x
Q

G

G

P

I

K

E

A

A

G
|
G

G

G

H

H

L

R

G

G

Y

V

K

F

N

E

D

P

Q

E

I

R

E

G

D

D

E

A

H

A

F

I

S

G

L

-

E

-

H

-

I

-

V

-

P

-

E

-

V

T

I

L

E

A

V

L

I

V

K

R

A

L

Y

L

E

A

K

A

S

R

G

G

H

-

C

-

K

S

I

L

P

P

V

V

I

V

A

A

A

A

G

G

G
|
G

L

I

Y

M

T

D

G

G

E

R

D

G

I

I

Y

R

R

A

L

A

M

L

Q

E

L

L

G

G

A

A

T

S

G

A

V

V

Q
|
Q

L
x
M

G
|
G

S
x
T

K

A

F

F

V

V

T

L

T

C

E

P

E

E

C

S

D

S

A

A

S

N

P

A

V

A

F

Y

K

R

Q

E

A

A

L21 (≠ V22) binding FMN
N69 (= N84) binding FMN
Q176 (≠ E160) binding FMN
G181 (= G165) binding FMN
G218 (= G211) binding FMN
QMGT 237:240 (≠ QLGS 230:233) binding FMN

3bw2A Crystal structures and site-directed mutagenesis study of nitroalkane oxidase from streptomyces ansochromogenes (see paper)
28% identity, 57% coverage: 155:358/359 of query aligns to 162:346/347 of 3bw2A

query
sites
3bw2A

D

D

A

A

I

V

V

I

V

A

E
x
Q

G

G

P

V

K

E

A
|
A

G
|
G

G
|
G

H

H

L

Q

G

G

Y

T

K

H

N

R

D

D

Q

S