SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_022669880.1 NCBI__GCF_000420385.1:WP_022669880.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

3bs8A Crystal structure of glutamate 1-semialdehyde aminotransferase complexed with pyridoxamine-5'-phosphate from bacillus subtilis (see paper)
53% identity, 98% coverage: 2:427/433 of query aligns to 4:428/430 of 3bs8A

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active site: V22 (= V20), Y145 (= Y143), E207 (= E205), D240 (= D238), M243 (= M241), K268 (= K266), G401 (≠ S400)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G118 (= G116), T119 (= T117), Y145 (= Y143), H146 (= H144), E207 (= E205), N212 (= N210), D240 (= D238), V242 (= V240), K268 (= K266)

P42799 Glutamate-1-semialdehyde 2,1-aminomutase 1, chloroplastic; AtGSA1; GSA 1; Glutamate-1-semialdehyde aminotransferase 1; GSA-AT 1; EC 5.4.3.8 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
52% identity, 98% coverage: 2:426/433 of query aligns to 50:474/474 of P42799

query
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P42799

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K314 (= K266) modified: N6-(pyridoxal phosphate)lysine

Q42522 Glutamate-1-semialdehyde 2,1-aminomutase 2, chloroplastic; GSA 2; Glutamate-1-semialdehyde aminotransferase 2; GSA-AT 2; EC 5.4.3.8 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
52% identity, 98% coverage: 2:426/433 of query aligns to 48:472/472 of Q42522

query
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Q42522

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N

F

T

T

L

I

V

A

A

A

V

A

E

E

E

K

V

V

F

L

R

S

E

R

L

L

R92 (≠ Y46) mutation to K: In gsa2-1; suppression of enf1 mutant pleiotropic developmental phenotypes; when associated with S-162.
G162 (= G116) mutation to S: In gsa2-1; suppression of enf1 mutant pleiotropic developmental phenotypes; when associated with K-92.

5hdmB Crystal structure of arabidopsis thaliana glutamate-1-semialdehyde-2, 1-aminomutase (see paper)
52% identity, 98% coverage: 2:426/433 of query aligns to 4:428/428 of 5hdmB

query
sites
5hdmB

F

L

E

Q

K

K

S

S

R

E

R

E

L

A

F

F

E

N

E

A

A

A

K

K

R

N

Y

L

I

M

V

P

G

G

G

G

V
|
V

N

N

S

S

P

P

V

V

R

R

A

A

F

F

K

K

N

S

V

V

G

G

R

G

T

Q

P

P

L

V

F

L

I

I

A

D

K

S

A

V

K

K

G

G

S

S

K

K

I

M

Y

W

D

D

E

I

D

D

G

G

N

N

E

E

F

Y

I

I

D

D

Y

Y

V

V

N

G

S

S

W

W

G

G

P

P

A

A

I

I

L

I

G

G

H

H

A

A

D

D

D

D

E

E

V

V

I

L

D

A

K

A

I

L

E

A

E

E

V

T

L

M

E

K

K

K

G

G

F

T

S

S

F

F

G

G

A

A

P

P

T

C

I

L

L

L

E

E

T

N

E

V

I

L

A

A

K

E

R

M

I

V

V

I

N

S

A

A

F

V

D

P

S

S

I

I

E

E

L

M

V

V

R

R

F

F

V

V

S

N

S

S

G
|
G

T
|
T

E

E

A

A

A

C

M

M

S

G

A

V

I

L

R

R

L

L

A

A

R

R

G

A

V

F

T

T

G

N

R

K

D

E

N

K

I

F

L

I

K

K

F

F

T

E

G

G

C

C

Y
|
Y

H

H

G

G

H

H

S

A

D

N

S

A

L

F

L

L

V

V

G

K

A

A

G

G

S

S

G

G

S

V

S

A

T

T

F

L

G

G

V

L

P

P

S

D

S

S

K

P

G

G

V

V

P

P

I

K

D

A

F

A

A

T

K

S

H

D

T

T

L

L

L

T

A

A

E

P

F

Y

N

N

D

D

L

L

D

E

A

A

V

V

E

E

D

K

I

L

F

F

K

A

K

A

Y

H

G

K

N

G

S

E

I

I

A

S

A

A

V

V

I

I

V

L

E
|
E

P

P

V

V

A

V

G

G

N
|
N

M

S

G

G

V

F

I

I

R

P

P

P

V

T

E

P

G

E

F

F

L

I

E

N

G

G

L

L

R

R

R

Q

V

L

C

T

D

K

D

D

Y

N

G

G

A

V

I

L

L

L

I

I

F

F

D
|
D

E

E

V
|
V

M
|
M

T

T

G

G

F

F

R

R

L

L

T

A

Y

Y

G

G

G

G

A

A

Q

Q

H

E

I

Y

Y

F

N

G

V

I

K

T

P

P

D

D

I

L

T

T

I

T

L

L

G

G

K
|
K

V

I

V

I

G

G

G

G

G

G

F

L

P

P

A

V

A

G

C

A

F

Y

G

G

A

G

R

R

K

R

E

D

I

I

M

M

E

E

Y

M

L

V

A

A

P

P

E

A

G

G

E

P

V

M

Y

Y

Q

Q

A

A

G

G

T

T

L

L

S

S

G

G

N

N

P

P

I

L

A

A

M

M

A

T

A

A

G

G

I

I

A

H

T

T

L

L

D

K

I

R

L

L

K

K

R

Q

K

A

N

G

P

T

Y

Y

E

E

E

Y

L

L

N

D

K

K

N

I

T

T

Q

K

K

E

I

L

I

T

S

N

R

G

I

I

I

L

G

E

L

A

G

G

V

K

E

K

Y

T

G

G

I

H

P

P

V

M

S

C

G

G

E

G

A

Y

I

I

G

S

S

G

M

M

F

F

S

G

I

F

F

F

F

F

K

A

E

E

D

G

K

P

P

V

K

Y

N

N

Y

F

S

A

E

D

V

S

S

K

K

K

S

S

D

D

Q

T

R

E

L

K

F

F

T

G

N

R

Y

F

F

F

I

R

G

G

L

M

L

L

E

E

R

E

G

G

I

V

Y

Y

I

F

P

A

P

P

S

S

Q

Q

F

F

E

E

S
x
A

N

G

F

F

I

T

T

S

I

L

K

A

H

H

S

T

L

P

A

E

D

D

I

I

E

Q

N

L

T

T

L

I

V

A

A

A

V

A

E

E

E

R

V

V

F

L

R

S

E

R

L

I

active site: V22 (= V20), Y145 (= Y143), E207 (= E205), D240 (= D238), M243 (= M241), K268 (= K266), A402 (≠ S400)
binding pyridoxal-5'-phosphate: G118 (= G116), T119 (= T117), Y145 (= Y143), E207 (= E205), N212 (= N210), D240 (= D238), V242 (= V240), M243 (= M241), K268 (= K266)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G118 (= G116), T119 (= T117), Y145 (= Y143), E207 (= E205), N212 (= N210), D240 (= D238), V242 (= V240), M243 (= M241), K268 (= K266)

5hdmA Crystal structure of arabidopsis thaliana glutamate-1-semialdehyde-2, 1-aminomutase (see paper)
52% identity, 98% coverage: 2:426/433 of query aligns to 4:428/428 of 5hdmA

query
sites
5hdmA

F

L

E

Q

K

K

S

S

R

E

R

E

L

A

F

F

E

N

E

A

A

A

K

K

R

N

Y

L

I

M

V

P

G

G

G

G

V
|
V

N

N

S

S

P

P

V

V

R

R

A

A

F

F

K

K

N

S

V

V

G

G

R

G

T

Q

P

P

L

V

F

L

I

I

A

D

K

S

A

V

K

K

G

G

S

S

K

K

I

M

Y

W

D

D

E

I

D

D

G

G

N

N

E

E

F

Y

I

I

D

D

Y

Y

V

V

N

G

S

S

W

W

G

G

P

P

A

A

I

I

L

I

G

G

H

H

A

A

D

D

D

D

E

E

V

V

I

L

D

A

K

A

I

L

E

A

E

E

V

T

L

M

E

K

K

K

G

G

F

T

S

S

F

F

G

G

A

A

P

P

T

C

I

L

L

L

E

E

T

N

E

V

I

L

A

A

K

E

R

M

I

V

V

I

N

S

A

A

F

V

D

P

S

S

I

I

E

E

L

M

V

V

R

R

F

F

V

V

S

N

S

S

G
|
G

T
|
T

E

E

A

A

A

C

M

M

S

G

A

V

I

L

R

R

L

L

A

A

R

R

G

A

V

F

T

T

G

N

R

K

D

E

N

K

I

F

L

I

K

K

F

F

T

E

G

G

C

C

Y
|
Y

H

H

G
|
G

H

H

S

A

D

N

S

A

L

F

L

L

V

V

G

K

A

A

G

G

S

S

G

G

S

V

S

A

T

T

F

L

G

G

V

L

P

P

S

D

S

S

K

P

G

G

V

V

P

P

I

K

D

A

F

A

A

T

K

S

H

D

T

T

L

L

L

T

A

A

E

P

F

Y

N

N

D

D

L

L

D

E

A

A

V

V

E

E

D

K

I

L

F

F

K

A

K

A

Y

H

G

K

N

G

S

E

I

I

A

S

A

A

V

V

I

I

V

L

E
|
E

P

P

V

V

A

V

G

G

N
|
N

M

S

G

G

V

F

I

I

R

P

P

P

V

T

E

P

G

E

F

F

L

I

E

N

G

G

L

L

R

R

R

Q

V

L

C

T

D

K

D

D

Y

N

G

G

A

V

I

L

L

L

I

I

F

F

D
|
D

E

E

V
|
V

M
|
M

T

T

G

G

F

F

R

R

L

L

T

A

Y

Y

G

G

G

G

A

A

Q

Q

H

E

I

Y

Y

F

N

G

V

I

K

T

P

P

D

D

I

L

T

T

I

T

L

L

G

G

K
|
K

V

I

V

I

G

G

G

G

G

G

F

L

P

P

A

V

A

G

C

A

F

Y

G

G

A

G

R

R

K

R

E

D

I

I

M

M

E

E

Y

M

L

V

A

A

P

P

E

A

G

G

E

P

V

M

Y

Y

Q

Q

A

A

G

G

T

T

L

L

S

S

G

G

N

N

P

P

I

L

A

A

M

M

A

T

A

A

G

G

I

I

A

H

T

T

L

L

D

K

I

R

L

L

K

K

R

Q

K

A

N

G

P

T

Y

Y

E

E

E

Y

L

L

N

D

K

K

N

I

T

T

Q

K

K

E

I

L

I

T

S

N

R

G

I

I

I

L

G

E

L

A

G

G

V

K

E

K

Y

T

G

G

I

H

P

P

V

M

S

C

G

G

E

G

A

Y

I

I

G

S

S

G

M

M

F

F

S

G

I

F

F

F

F

F

K

A

E

E

D

G

K

P

P

V

K

Y

N

N

Y

F

S

A

E

D

V

S

S

K

K

K

S

S

D

D

Q

T

R

E

L

K

F

F

T

G

N

R

Y

F

F

F

I

R

G

G

L

M

L

L

E

E

R

E

G

G

I

V

Y

Y

I

F

P

A

P

P

S

S

Q

Q

F

F

E

E

S
x
A

N

G

F

F

I

T

T

S

I

L

K

A

H

H

S

T

L

P

A

E

D

D

I

I

E

Q

N

L

T

T

L

I

V

A

A

A

V

A

E

E

E

R

V

V

F

L

R

S

E

R

L

I

active site: V22 (= V20), Y145 (= Y143), E207 (= E205), D240 (= D238), M243 (= M241), K268 (= K266), A402 (≠ S400)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G118 (= G116), T119 (= T117), Y145 (= Y143), G147 (= G145), E207 (= E205), N212 (= N210), D240 (= D238), V242 (= V240), K268 (= K266)

P23893 Glutamate-1-semialdehyde 2,1-aminomutase; GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT; EC 5.4.3.8 from Escherichia coli (strain K12) (see paper)
51% identity, 98% coverage: 2:426/433 of query aligns to 1:426/426 of P23893

query
sites
P23893

F

M

E

S

K

K

S

S

R

E

R

N

L

L

F

Y

E

S

E

A

A

A

K

R

R

E

Y

L

I

I

V

P

G

G

G

G

V

V

N

N

S

S

P

P

V

V

R

R

A

A

F

F

K

T

N

G

V

V

G

G

R

G

T

T

P

P

L

L

F

F

I

I

A

E

K

K

A

A

K

D

G

G

S

A

K

Y

I

L

Y

Y

D

D

E

V

D

D

G

G

N

K

E

A

F

Y

I

I

D

D

Y

Y

V

V

N

G

S

S

W

W

G

G

P

P

A

M

I

V

L

L

G

G

H

H

A

N

D

H

D

P

E

A

V

I

I

R

D

N

K

A

I

V

E

I

E

E

V

A

L

A

E

E

K

R

G

G

F

L

S

S

F

F

G

G

A

A

P

P

T

T

I

E

L

M

E

E

T

V

E

K

I

M

A

A

K

Q

R

L

I

V

V

T

N

E

A

L

F

V

D

P

S

T

I

M

E

D

L

M

V

V

R

R

F

M

V

V

S

N

S

S

G

G

T

T

E

E

A

A

A

T

M

M

S

S

A

A

I

I

R

R

L

L

A

A

R

R

G

G

V

F

T

T

G

G

R

R

D

D

N

K

I

I

L

I

K

K

F

F

T

E

G

G

C

C

Y

Y

H

H

G

G

H

H

S

A

D

D

S

C

L

L

L

L

V

V

G

K

A

A

G

G

S

S

G

G

S

A

S

L

T

T

F

L

G

G

V

Q

P

P

S

N

S

S

K

P

G

G

V

V

P

P

I

A

D

D

F

F

A

A

K

K

H

Y

T

T

L

L

L

T

A

C

E

T

F

Y

N

N

D

D

L

L

D

A

A

S

V

V

E

R

D

A

I

A

F

F

K

E

K

Q

Y

Y

G

P

N

Q

S

E

I

I

A

A

A

C

V

I

I

I

V

V

E

E

P

P

V

V

A

A

G

G

N

N

M

M

G

N

V

C

I

V

R

P

P

P

V

L

E

P

G

E

F

F

L

L

E

P

G

G

L

L

R

R

R

A

V

L

C

C

D

D

D

E

Y

F

G

G

A

A

I

L

L

L

I

I

F

I

D

D

E

E

V

V

M

M

T

T

G

G

F

F

R

R

L

V

T

A

Y

L

G

A

G

G

A

A

Q

Q

H

D

I

Y

Y

Y

N

G

V

V

K

V

P

P

D

D

I

L

T

T

I

C

L

L

G

G

K
|
K

V

I

V

I

G

G

G

G

G

G

F

M

P

P

A

V

A

G

C

A

F

F

G

G

A

G

R

R

K

R

E

D

I

V

M

M

E

D

Y

A

L

L

A

A

P

P

E

T

G

G

E

P

V

V

Y

Y

Q

Q

A

A

G

G

T

T

L

L

S

S

G

G

N

N

P

P

I

I

A

A

M

M

A

A

A

A

G

G

I

F

A

A

T

C

L

L

D

N

I

E

L

V

K

A

R

Q

K

P

N

G

P

V

Y

H

E

E

E

T

L

L

N

D

K

E

N

L

T

T

Q

T

K

R

I

L

I

A

S

E

R

G

I

L

I

L

G

E

L

A

G

A

V

E

E

E

Y

A

G

G

I

I

P

P

V

L

S

V

G

V

E

N

A

H

I

V

G

G

S

G

M

M

F

F

S

G

I

I

F

F

F

F

K

T

E

D

-

A

D

E

K

S

P

V

K

T

N

C

Y

Y

S

Q

E

D

V

V

S

M

K

A

S

C

D

D

Q

V

R

E

L

R

F

F

T

K

N

R

Y

F

F

F

I

H

G

M

L

M

L

L

E

D

R

E

G

G

I

V

Y

Y

I

L

P

A

P

P

S

S

Q

A

F

F

E

E

S

A

N

G

F

F

I

M

T

S

I

V

K

A

H

H

S

S

L

M

A

E

D

D

I

I

E

N

N

N

T

T

L

I

V

D

A

A

V

A

E

R

E

R

V

V

F

F

R

A

E

K

L

L

K265 (= K266) mutation to R: 2% of wild-type activity.

2e7uA Crystal structure of glutamate-1-semialdehyde 2,1-aminomutase from thermus thermophilus hb8
51% identity, 97% coverage: 5:423/433 of query aligns to 6:424/424 of 2e7uA

query
sites
2e7uA

S

S

R

E

R

A

L

Y

F

F

E

Q

E

E

A

A

K

K

R

R

Y

H

I

I

V

P

G

G

G

G

V
|
V

N

S

S

S

P

P

V

V

R

R

A

A

F

F

K

K

N

A

V

V

G

G

R

G

T

T

P

P

L

P

F

F

I

L

A

V

K

R

A

G

K

E

G

G

S

A

K

Y

I

V

Y

W

D

D

E

A

D

D

G

G

N

N

E

R

F

Y

I

L

D

D

Y

Y

V

V

N

M

S

S

W

W

G

G

P

P

A

L

I

I

L

L

G

G

H

H

A

A

D

H

D

P

E

K

V

V

I

L

D

A

K

R

I

V

E

R

E

E

V

T

L

L

E

E

K

R

G

G

F

L

S

T

F

F

G

G

A

A

P

P

T

S

I

P

L

L

E

E

T

V

E

A

I

L

A

A

K

K

R

K

I

V

V

K

N

R

A

A

F

Y

D

P

S

F

I

V

E

D

L

L

V

V

R

R

F

F

V

V

S

N

S

S

G
|
G

T
|
T

E

E

A

A

A

T

M

M

S

S

A

A

I

L

R

R

L

L

A

A

R

R

G

G

V

Y

T

T

G

G

R

R

D

P

N

Y

I

I

L

V

K

K

F

F

T

R

G

G

C

N

Y
|
Y

H
|
H

G

G

H

H

S

A

D

D

S

G

L

L

L

L

V

V

G

E

A

A

G

G

S

S

G

G

S

A

S

L

T

T

F

L

G

G

V

V

P

P

S

S

S

S

K

A

G

G

V

V

P

P

I

E

D

E

F

Y

A

A

K

K

H

L

T

T

L

L

L

V

A

L

E

E

F

Y

N

N

D

D

L

P

D

E

A

G

V

L

E

R

D

E

I

V

F

L

K

K

K

R

Y

R

G

G

N

E

S

E

I

I

A

A

A

A

V

I

I

I

V

F

E
|
E

P

P

V

V

A

V

G

G

N
|
N

M

A

G

G

V

V

I

L

R

V

P

P

V

T

E

E

G

D

F

F

L

L

E

K

G

A

L

L

R

H

R

E

V

-

C

A

D

K

D

A

Y

Y

G

G

A

V

I

L

L

L

I

I

F

A

D
|
D

E

E

V
|
V

M
|
M

T

T

G

G

F

F

R

R

L

L

T

A

Y

F

G

G

G

G

A

A

Q

T

H

E

I

L

Y

L

N

G

V

L

K

K

P

P

D

D

I

L

T

V

I

T

L

L

G

G

K
|
K

V

I

V

L

G

G

G

G

G

G

F

L

P

P

A

A

A

A

C

A

F

Y

G

A

A

G

R

R

K

R

E

E

I

I

M

M

E

E

Y

K

L

V

A

A

P

P

E

L

G

G

E

P

V

V

Y

Y

Q

Q

A

A

G

G

T

T

L

L

S

S

G

G

N

N

P

P

I

L

A

A

M

M

A

A

A

A

G

G

I

L

A

A

T

T

L

L

D

E

I

L

L

L

K

E

R

E

K

N

N

P

P

G

Y

Y

-

Y

-

A

-

Y

-

L

E

E

E

D

L

L

N

G

K

A

N

R

T

L

Q

E

K

A

I

G

I

L

S

K

R

E

I

V

I

L

G

K

L

-

G

-

V

-

E

E

Y

K

G

G

I

L

P

P

V

H

S

T

G

V

E

N

A

R

I

V

G

G

S

S

M

M

F

I

S

T

I

V

F

F

F

F

K

T

E

E

D

G

K

P

P

V

K

V

N

T

Y

F

S

Q

E

D

V

A

S

R

K

R

S

T

D

D

Q

T

R

E

L

L

F

F

T

K

N

R

Y

F

F

F

I

H

G

G

L

L

L

L

E

D

R

R

G

G

I

I

Y

Y

I

W

P

P

P

P

S

S

Q

N

F

F

E

E

S
x
A

N

A

F

F

I

L

T

S

I

V

K

A

H

H

S

R

L

E

A

E

D

D

I

V

E

E

N

K

T

T

L

L

V

E

A

A

V

L

E

R

E

K

V

A

F

L

active site: V21 (= V20), Y144 (= Y143), E206 (= E205), D238 (= D238), M241 (= M241), K266 (= K266), A401 (≠ S400)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G117 (= G116), T118 (= T117), Y144 (= Y143), H145 (= H144), N211 (= N210), D238 (= D238), V240 (= V240)

5i92F Crystal structure of glutamate-1-semialdehyde 2,1- aminomutase (gsa) from pseudomonas aeruginosa
51% identity, 97% coverage: 2:423/433 of query aligns to 1:418/420 of 5i92F

query
sites
5i92F

F

M

E

S

K

R

S

S

R

E

R

T

L

L

F

F

E

N

E

N

A

A

K

Q

R

K

Y

H

I

I

V

P

G

G

G

G

V
|
V

N

N

S

S

P

P

V

V

R

R

A

A

F

F

K

K

N

S

V

V

G

G

R

G

T

T

P

P

L

L

F

F

I

F

A

K

K

H

A

A

K

E

G

G

S

A

K

Y

I

V

Y

L

D

D

E

E

D

D

G

D

N

K

E

R

F

Y

I

V

D

D

Y

Y

V

V

N

G

S

S

W

W

G

G

P

P

A

M

I

I

L

L

G

G

H

H

A

S

D

H

D

P

E

D

V

V

I

L

D

D

K

A

I

V

E

R

E

R

V

Q

L

L

E

D

K

H

G

G

F

L

S

S

F

Y

G

G

A

A

P

P

T

T

I

A

L

L

E

E

T

V

E

E

I

M

A

A

K

D

R

L

I

V

V

C

N

S

A

M

F

V

D

P

S

S

I

M

E

E

L

M

V

V

R

R

F

M

V

V

S

S

S

S

G

G

T

T

E

E

A

A

A

T

M

M

S

S

A

A

I

I

R

R

L

L

A

A

R

R

G

G

V

Y

T

T

G

G

R

R

D

D

N

S

I

I

L

I

K

K

F

F

T

E

G

G

C

C

Y
|
Y

H

H

G

G

H

H

S

S

D

D

S

S

L

L

L

L

V

V

G

K

A

A

G

G

S

-

G

-

S

-

S

S

T

T

F

F

G

G

V

V

P

P

S

N

S

S

K

P

G

G

V

V

P

P

I

A

D

A

F

F

A

A

K

K

H

H

T

T

L

L

L

T

A

L

E

P

F

F

N

N

D

D

L

I

D

E

A

A

V

V

E

R

D

K

I

T

F

L

K

G

K

E

Y

V

G

G

N

K

S

E

I

V

A

A

A

C

V

I

I

I

V

V

E
|
E

P

P

V

V

A

A

G

G

N

N

M

M

G

N

V

C

I

V

R

P

P

P

V

A

E

P

G

G

F

F

L

L

E

E

G

G

L

L

R

R

R

E

V

A

C

C

D

D

D

E

Y

H

G

G

A

V

I

V

L

L

I

I

F

F

D
|
D

E

E

V

V

M
|
M

T

T

G

G

F

F

R

R

L

V

T

A

Y

L

G

G

G

G

A

A

Q

Q

H

A

I

Y

Y

Y

N

G

V

V

K

T

P

P

D

D

I

L

T

S

I

T

L

F

G

G

K
|
K

V

I

V

I

G

G

G

G

G

G

F

M

P

P

A

V

A

G

C

A

F

F

G

G

A

G

R

K

K

R

E

E

I

I

M

M

E

Q

Y

Q

L

I

A

S

P

P

E

L

G

G

E

P

V

V

Y

Y

Q

Q

A

A

G

G

T

T

L

-

S

-

G

G

N

N

P

P

I

L

A

A

M

M

A

A

A

A

G

G

I

L

A

T

T

T

L

L

D

R

I

L

L

I

K

S

R

R

K

P

N

G

P

F

Y

H

E

D

E

E

L

L

N

T

K

A

N

Y

T

T

Q

T

K

R

I

M

I

L

S

D

R

G

I

L

I

Q

G

Q

L

R

G

A

V

D

E

A

Y

A

G

G

I

I

P

P

V

F

S

V

G

T

E

T

A

Q

I

A

G

G

S

G

M

M

F

F

S

G

I

L

F

Y

F

F

K

S

-

G

E

A

D

D

K

A

P

I

K

V

N

T

Y

F

S

E

E

D

V

V

S

M

K

A

S

S

D

D

Q

V

R

E

L

R

F

F

T

K

N

R

Y

F

F

F

I

H

G

L

L

M

L

L

E

D

R

G

G

G

I

V

Y

Y

I

L

P

A

P

P

S

S

Q

A

F

F

E

E

S
x
A

N

G

F

F

I

T

T

S

I

I

K

A

H

H

S

G

L

D

A

K

D

E

I

L

E

E

N

I

T

T

L

L

V

N

A

A

V

A

E

E

E

K

V
x
A

F
|
F

active site: V19 (= V20), Y142 (= Y143), E201 (= E205), D234 (= D238), M237 (= M241), K262 (= K266), A395 (≠ S400)
binding leucine: A417 (≠ V422), F418 (= F423)

Sites not aligning to the query:

binding leucine: 420

3k28A Crystal structure of a glutamate-1-semialdehyde aminotransferase from bacillus anthracis with bound pyridoxal 5'phosphate
53% identity, 95% coverage: 2:411/433 of query aligns to 4:407/423 of 3k28A

query
sites
3k28A

F

F

E

D

K

K

S

S

R

I

R

A

L

A

F

F

E

E

E

E

A

A

K

Q

R

D

Y

L

I

M

V

P

G

G

G

G

V
|
V

N

N

S

S

P

P

V

V

R

R

A

A

F

F

K

K

N

S

V

V

G

G

R

M

T

N

P

P

L

L

F

F

I

M

A

E

K

R

A

G

K

K

G

G

S

S

K

K

I

V

Y

Y

D

D

E

I

D

D

G

G

N

N

E

E

F

Y

I

I

D

D

Y

Y

V

V

N

L

S

S

W

W

G

G

P

P

A

L

I

I

L

H

G

G

H

H

A

A

D

N

D

D

E

R

V

V

I

V

D

E

K

A

I

L

E

K

E

A

V

V

L

A

E

E

K

R

G

G

F

T

S

S

F

F

G

G

A

A

P

P

T

T

I

E

L

I

E

E

T

N

E

K

I

L

A

A

K

K

R

L

I

V

V
x
I

N

E

A

R

F
x
V

D
x
P

S

S

I
|
I

E

E

L

I

V

V

R

R

F

M

V

V

S

N

S

S

G
|
G

T
|
T

E

E

A

A

A

T

M

M

S

S

A

A

I

L

R

R

L

L

A

A

R

R

G

G

V

Y

T

T

G

G

R

R

D

N

N

K

I

I

L

L

K

K

F

F

T

I

G

G

C

C

Y
|
Y

H
|
H

G
|
G

H

H

S

G

D

D

S

S

L

L

L

L

V

I

G

K

A

A

G

G

S

S

G

-

S

-

S

-

T

-

F

-

G

G

V

V

P

P

S

D

S

S

K

P

G

G

V

V

P

P

I

E

D

G

F

V

A

A

K

K

H

N

T

T

L

I

L

T

A

V

E

A

F

Y

N

N

D

D

L

L

D

E

A

S

V

V

E

K

D

Y

I

A

F

F

K

E

K

Q

Y

F

G

G

N

D

S

D

I

I

A

A

A

C

V

V

I

I

V

V

E
|
E

P

P

V

V

A

A

G

G

N

N

M

M

G

G

V

V

I

V

R

P

P

P

V

Q

E

P

G

G

F

F

L

L

E

E

G

G

L

L

R

R

R

E

V

V

C

T

D

E

D

Q

Y

N

G

G

A

A

I

L

L

L

I

I

F

F

D
|
D

E

E

V
|
V

M
|
M

T

T

G

G

F

F

R

R

L

V

T

A

Y

Y

G

N

G

C

A

G

Q

Q

H

G

I

Y

Y

Y

N

G

V

V

K

T

P

P

D

D

I

L

T

T

I

C

L

L

G

G

K
|
K

V

V

V

I

G

G

G

G

G

G

F

L

P

P

A

V

A

G

C

A

F

Y

G

G

A

G

R

K

K

A

E

E

I

I

M

M

E

R

Y

Q

L

V

A

A

P

P

E

S

G

G

E

P

V

I

Y

Y

Q

Q

A

A

G

G

T

T

L

L

S

S

G

G

N

N

P

P

I

L

A

A

M

M

A

A

A

A

G

G

I

Y

A

E

T

T

L

L

D

V

I

Q

L

L

K

T

R

P

K

E

N

S

P

-

Y

Y

E

V

E

E

L

F

N

E

K

R

N

K

T

A

Q

E

K

M

I

L

I

E

S

A

R

G

I

L

I

R

G

K

L

A

G

A

V

E

E

K

Y

H

G

G

I

I

P

P

V

H

S

H

G

I

E

N

A

R

I

A

G

G

S

S

M

M

F

I

S

G

I

I

F

F

F

F

K

T

E

D

D

E

K

P

P

V

K

I

N

N

Y

Y

S

D

E

A

V

A

S

K

K

S

S

S

D

N

Q

L

R

Q

L

F

F

F

T

A

N

A

Y

Y

F

Y

I

R

G

E

L

M

L

V

E

E

R

Q

G

G

I

V

Y

F

I

L

P

P

P

P

S

S

Q

Q

F

F

E

E

S
x
G

N

L

F

F

I

L

T

S

I

T

K

V

H

H

S

S

L

D

A

A

D

D

active site: V22 (= V20), Y145 (= Y143), E202 (= E205), D235 (= D238), M238 (= M241), K263 (= K266), G396 (≠ S400)
binding calcium ion: I103 (≠ V101), V106 (≠ F104), P107 (≠ D105), I109 (= I107)
binding pyridoxal-5'-phosphate: G118 (= G116), T119 (= T117), Y145 (= Y143), H146 (= H144), G147 (= G145), E202 (= E205), D235 (= D238), V237 (= V240), M238 (= M241), K263 (= K266)

2gsaB Crystal structure of glutamate-1-semialdehyde aminomutase (aminotransferase, wild-type form) (see paper)
49% identity, 98% coverage: 4:426/433 of query aligns to 5:427/427 of 2gsaB

query
sites
2gsaB

K

K

S

S

R

D

R

E

L

I

F

F

E

A

E

A

A

A

K

Q

R

K

Y

L

I

M

V

P

G

G

G

G

V
|
V

N

S

S

S

P

P

V

V

R

R

A

A

F

F

K

K

N

S

V

V

G

G

R

G

T

Q

P

P

L

I

F

V

I

F

A

D

K

R

A

V

K

K

G

D

S

A

K

Y

I

A

Y

W

D

D

E

V

D

D

G

G

N

N

E

R

F

Y

I

I

D

D

Y

Y

V

V

N

G

S

T

W

W

G

G

P

P

A

A

I

I

L

C

G

G

H

H

A

A

D

H

D

P

E

E

V

V

I

I

D

E

K

A

I

L

E

K

E

V

V

A

L

M

E

E

K

K

G

G

F

T

S

S

F

F

G

G

A

A

P

P

T

C

I

A

L

L

E

E

T

N

E

V

I

L

A

A

K

E

R

M

I

V

V

N

N

D

A

A

F

V

D

P

S

S

I

I

E

E

L

M

V

V

R

R

F

F

V

V

S

N

S

S

G
|
G

T
|
T

E

E

A

A

A

C

M

M

S

A

A

V

I

L

R

R

L

L

A

M

R

R

G

A

V

Y

T

T

G

G

R

R

D

D

N

K

I

I

L

I

K

K

F

F

T

E

G

G

C

C

Y
|
Y

H

H

G

G

H

H

S

A

D

D

S

M

L

F

L

L

V

V

G

K

A

A

G

G

S

S

G

G

S

V

S

A

T

T

F

L

G

G

V

L

P

P

S

S

S

S

K

P

G

G

V

V

P

P

I

K

D

K

F

T

A

T

K

A

H

N

T

T

L

L

L

T

A

T

E

P

F

Y

N

N

D

D

L

L

D

E

A

A

V

V

E

K

D

A

I

L

F

F

K

A

K

E

Y

N

G

P

N

G

S

E

I

I

A

A

A

G

V

V

I

I

V

L

E
|
E

P

P

V

I

A

V

G

G

N
|
N

M

S

G

G

V

F

I

I

R

V

P

P

V

D

E

A

G

G

F

F

L

L

E

E

G

G

L

L

R

R

R

E

V

I

C

T

D

L

D

E

Y

H

G

D

A

A

I

L

L

L

I

V

F

F

D
|
D

E

E

V
|
V

M
|
M

T

T

G

G

F

F

R

R

L

I

T

A

Y

Y

G

G

G

G

A

V

Q

Q

H

E

I

K

Y

F

N

G

V

V

K

T

P

P

D

D

I

L

T

T

I

T

L

L

G

G

K
|
K

V

I

V

I

G

G

G

G

G

G

F

L

P

P

A

V

A

G

C

A

F

Y

G

G

A

G

R

K

K

R

E

E

I

I

M

M

E

Q

Y

L

L

V

A

A

P

P

E

A

G

G

E

P

V

M

Y

Y

Q

Q

A

A

G

G

T

T

L

L

S

S

G

G

N

N

P

P

I

L

A

A

M

M

A

T

A

A

G

G

I

I

A

K

T

T

L

L

D

E

I

L

L

L

K

R

R

Q

K

P

N

G

P

T

Y

Y

E

E

E

Y

L

L

N

D

K

Q

N

I

T

T

Q

K

K

R

I

L

I

S

S

D

R

G

I

L

I

L

G

A

L

I

G

A

V

Q

E

E

Y

T

G

G

I

H

P

A

V

A

S

C

G

G

E

G

A

Q

I

V

G

S

S

G

M

M

F

F

S

G

I

F

F

F

F

F

K

T

E

E

D

G

K

P

P

V

K

H

N

N

Y

Y

S

E

E

D

V

A

S

K

K

K

S

S

D

D

Q

L

R

Q

L

K

F

F

T

S

N

R

Y

F

F

H

I

R

G

G

L

M

L

L

E

E

R

Q

G

G

I

I

Y

Y

I

L

P

A

P

P

S

S

Q

Q

F

F

E

E

S
x
A

N

G

F

F

I

T

T

S

I

L

K

A

H

H

S

T

L

E

A

E

D

D

I

I

E

D

N

A

T

T

L

L

V

A

A

A

V

A

E

R

E

T

V

V

F

M

R

S

E

A

L

L

active site: V21 (= V20), Y144 (= Y143), E206 (= E205), D239 (= D238), M242 (= M241), K267 (= K266), A401 (≠ S400)
binding pyridoxal-5'-phosphate: G117 (= G116), T118 (= T117), Y144 (= Y143), E206 (= E205), N211 (= N210), D239 (= D238), V241 (= V240), M242 (= M241), K267 (= K266)

2gsaA Crystal structure of glutamate-1-semialdehyde aminomutase (aminotransferase, wild-type form) (see paper)
49% identity, 98% coverage: 4:426/433 of query aligns to 5:427/427 of 2gsaA

query
sites
2gsaA

K

K

S

S

R

D

R

E

L

I

F

F

E

A

E

A

A

A

K

Q

R

K

Y

L

I

M

V

P

G

G

G

G

V
|
V

N

S

S

S

P

P

V

V

R

R

A

A

F

F

K

K

N

S

V

V

G

G

R

G

T

Q

P

P

L

I

F

V

I

F

A

D

K

R

A

V

K

K

G

D

S

A

K

Y

I

A

Y

W

D

D

E

V

D

D

G

G

N

N

E

R

F

Y

I

I

D

D

Y

Y

V

V

N

G

S

T

W

W

G

G

P

P

A

A

I

I

L

C

G

G

H

H

A

A

D

H

D

P

E

E

V

V

I

I

D

E

K

A

I

L

E

K

E

V

V

A

L

M

E

E

K

K

G

G

F

T

S

S

F

F

G

G

A

A

P

P

T

C

I

A

L

L

E

E

T

N

E

V

I

L

A

A

K

E

R

M

I

V

V

N

N

D

A

A

F

V

D

P

S

S

I

I

E

E

L

M

V

V

R

R

F

F

V

V

S

N

S

S

G
|
G

T
|
T

E

E

A

A

A

C

M

M

S

A

A

V

I

L

R

R

L

L

A

M

R

R

G

A

V

Y

T

T

G

G

R

R

D

D

N

K

I

I

L

I

K

K

F

F

T

E

G

G

C

C

Y
|
Y

H
|
H

G
|
G

H

H

S

A

D

D

S

M

L

F

L

L

V

V

G

K

A

A

G

G

S

S

G

G

S

V

S

A

T

T

F

L

G

G

V

L

P

P

S

S

S

S

K

P

G

G

V

V

P

P

I

K

D

K

F

T

A

T

K

A

H

N

T

T

L

L

L

T

A

T

E

P

F

Y

N

N

D

D

L

L

D

E

A

A

V

V

E

K

D

A

I

L

F

F

K

A

K

E

Y

N

G

P

N

G

S

E

I

I

A

A

A

G

V

V

I

I

V

L

E
|
E

P

P

V

I

A

V

G

G

N
|
N

M

S

G

G

V

F

I

I

R

V

P

P

V

D

E

A

G

G

F

F

L

L

E

E

G

G

L

L

R

R

R

E

V

I

C

T

D

L

D

E

Y

H

G

D

A

A

I

L

L

L

I

V

F

F

D
|
D

E

E

V
|
V

M
|
M

T

T

G

G

F

F

R

R

L

I

T

A

Y

Y

G

G

G

G

A

V

Q

Q

H

E

I

K

Y

F

N

G

V

V

K

T

P

P

D

D

I

L

T

T

I

T

L

L

G

G

K
|
K

V

I

V

I

G

G

G

G

G

G

F

L

P

P

A

V

A

G

C

A

F

Y

G

G

A

G

R

K

K

R

E

E

I

I

M

M

E

Q

Y

L

L

V

A

A

P

P

E

A

G

G

E

P

V

M

Y

Y

Q

Q

A

A

G

G

T

T

L

L

S

S

G

G

N

N

P

P

I

L

A

A

M

M

A

T

A

A

G

G

I

I

A

K

T

T

L

L

D

E

I

L

L

L

K

R

R

Q

K

P

N

G

P

T

Y

Y

E

E

E

Y

L

L

N

D

K

Q

N

I

T

T

Q

K

K

R

I

L

I

S

S

D

R

G

I

L

I

L

G

A

L

I

G

A

V

Q

E

E

Y

T

G

G

I

H

P

A

V

A

S

C

G

G

E

G

A

Q

I

V

G

S

S

G

M

M

F

F

S

G

I

F

F

F

F

F

K

T

E

E

D

G

K

P

P

V

K

H

N

N

Y

Y

S

E

E

D

V

A

S

K

K

K

S

S

D

D

Q

L

R

Q

L

K

F

F

T

S

N

R

Y

F

F

H

I

R

G

G

L

M

L

L

E

E

R

Q

G

G

I

I

Y

Y

I

L

P

A

P

P

S

S

Q

Q

F

F

E

E

S
x
A

N

G

F

F

I

T

T

S

I

L

K

A

H

H

S

T

L

E

A

E

D

D

I

I

E

D

N

A

T

T

L

L

V

A

A

A

V

A

E

R

E

T

V

V

F

M

R

S

E

A

L

L

active site: V21 (= V20), Y144 (= Y143), E206 (= E205), D239 (= D238), M242 (= M241), K267 (= K266), A401 (≠ S400)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G117 (= G116), T118 (= T117), Y144 (= Y143), H145 (= H144), G146 (= G145), N211 (= N210), D239 (= D238), V241 (= V240), K267 (= K266)

3usfA Crystal structure of dava-4
49% identity, 98% coverage: 4:426/433 of query aligns to 5:427/427 of 3usfA

query
sites
3usfA

K

K

S

S

R

D

R

E

L

I

F

F

E

A

E

A

A

A

K

Q

R

K

Y

L

I

M

V

P

G

G

G

G

V
|
V

N

S

S
|
S

P

P

V
|
V

R

R

A

A

F

F

K

K

N

S

V

V

G

G

R

G

T

Q

P

P

L

I

F

V

I

F

A

D

K

R

A

V

K

K

G

D

S

A

K

Y

I

A

Y

W

D

D

E

V

D

D

G

G

N

N

E

R

F

Y

I

I

D

D

Y

Y

V

V

N

G

S

T

W

W

G

G

P

P

A

A

I

I

L

C

G

G

H

H

A

A

D

H

D

P

E

E

V

V

I

I

D

E

K

A

I

L

E

K

E

V

V

A

L

M

E

E

K

K

G

G

F

T

S

S

F

F

G

G

A

A

P

P

T

C

I

A

L

L

E

E

T

N

E

V

I

L

A

A

K

E

R

M

I

V

V

N

N

D

A

A

F

V

D

P

S

S

I

I

E

E

L

M

V

V

R

R

F

F

V

V

S

N

S

S

G
|
G

T
|
T

E

E

A

A

A

C

M

M

S

A

A

V

I

L

R

R

L

I

A

M

R

R

G

A

V

Y

T

T

G

G

R

R

D

D

N

K

I

I

L

I

K

K

F

F

T

E

G

G

C

C

Y
|
Y

H

H

G

G

H

H

S

A

D

D

S

M

L

F

L

L

V

V

G

K

A

A

G

G

S
|
S

G

G

S

V

S

A

T

T

F

L

G

G

V

L

P

P

S

S

S

S

K

P

G

G

V

V

P

P

I

K

D

K

F

T

A

T

K

A

H

N

T

T

L

L

L

T

A

T

E

P

F

Y

N

N

D

D

L

L

D

E

A

A

V

V

E

K

D

A

I

L

F

F

K

A

K

E

Y

N

G

P

N

G

S

E

I

I

A

A

A

G

V

V

I

I

V

L

E

E

P

P

V

I

A

V

G

G

N
|
N

M

S

G

G

V

F

I

I

R

V

P

P

V

D

E

A

G

G

F

F

L

L

E

E

G

G

L

L

R

R

R

E

V

I

C

T

D

L

D

E

Y

H

G

D

A

A

I

L

L

L

I

V

F

F

D
|
D

E

E

V
|
V

M
|
M

T

T

G

G

F

F

R

R

L

I

T

A

Y

Y

G

G

G

G

A

V

Q

Q

H

E

I

K

Y

F

N

G

V

V

K

T

P

P

D

D

I

L

T

T

I

T

L

L

G

G

K
|
K

V

I

V

I

G

G

G

G

G

G

F

L

P

P

A

V

A

G

C

A

F

Y

G

G

A

G

R

K

K

R

E

E

I

I

M

M

E

Q

Y

L

L

V

A

A

P

P

E

A

G

G

E

P

V

M

Y

Y

Q

Q

A

A

G

G

T

T

L

L

S

S

G

G

N

N

P

P

I

L

A

A

M

M

A

T

A

A

G

G

I

I

A

K

T

T

L

L

D

E

I

L

L

L

K

R

R

Q

K

P

N

G

P

T

Y

Y

E

E

E

Y

L

L

N

D

K

Q

N

I

T

T

Q

K

K

R

I

L

I

S

S

D

R

G

I

L

I

L

G

A

L

I

G

A

V

Q

E

E

Y

T

G

G

I

H

P

A

V

A

S

C

G

G

E

G

A

Q

I

V

G

S

S

G

M

M

F

F

S

G

I

F

F

F

F

F

K

T

E

E

D

G

K

P

P

V

K

H

N

N

Y

Y

S

E

E

D

V

A

S

K

K

K

S

S

D

D

Q

L

R

Q

L

K

F

F

T

S

N

R

Y

F

F

H

I

R

G

G

L

M

L

L

E

E

R

Q

G

G

I

I

Y

Y

I

L

P

A

P

P

S

S

Q

Q

F

F

E
|
E

S
x
A

N

G

F

F

I

T

T

S

I

L

K

A

H

H

S

T

L

E

A

E

D

D

I

I

E

D

N

A

T

T

L

L

V

A

A

A

V

A

E

R

E

T

V

V

F

M

R

S

E

A

L

L

active site: V21 (= V20), Y144 (= Y143), D239 (= D238), M242 (= M241), K267 (= K266), A401 (≠ S400)
binding (4s)-4,5-diaminopentanoic acid: S23 (= S22), V25 (= V24), S157 (= S156), K267 (= K266), E400 (= E399)
binding (5-hydroxy-4,6-dimethylpyridin-3-yl)methyl dihydrogen phosphate: G117 (= G116), T118 (= T117), Y144 (= Y143), N211 (= N210), D239 (= D238), V241 (= V240), K267 (= K266)

3fq7A Gabaculine complex of gsam (see paper)
49% identity, 98% coverage: 4:426/433 of query aligns to 5:427/427 of 3fq7A

query
sites
3fq7A

K

K

S

S

R

D

R

E

L

I

F

F

E

A

E

A

A

A

K

Q

R

K

Y

L

I

M

V

P

G

G

G

G

V
|
V

N

S

S
|
S

P

P

V
|
V

R

R

A

A

F

F

K

K

N

S

V

V

G

G

R

G

T

Q

P

P

L

I

F

V

I

F

A

D

K

R

A

V

K

K

G

D

S

A

K

Y

I

A

Y

W

D

D

E

V

D

D

G

G

N

N

E

R

F

Y

I

I

D

D

Y

Y

V

V

N

G

S

T

W
|
W

G

G

P

P

A

A

I

I

L

C

G

G

H

H

A

A

D

H

D

P

E

E

V

V

I

I

D

E

K

A

I

L

E

K

E

V

V

A

L

M

E

E

K

K

G

G

F

T

S

S

F

F

G

G

A

A

P

P

T

C

I

A

L

L

E

E

T

N

E

V

I

L

A

A

K

E

R

M

I

V

V

N

N

D

A

A

F

V

D

P

S

S

I

I

E

E

L

M

V

V

R

R

F

F

V

V

S

N

S

S

G
|
G

T
|
T

E

E

A

A

A

C

M

M

S

A

A

V

I

L

R

R

L

I

A

M

R

R

G

A

V

Y

T

T

G

G

R

R

D

D

N

K

I

I

L

I

K

K

F

F

T

E

G

G

C

C

Y
|
Y

H
|
H

G

G

H

H

S

A

D

D

S

M

L

F

L

L

V

V

G

K

A

A

G

G

S

S

G

G

S

V

S

A

T

T

F

L

G

G

V

L

P

P

S

S

S

S

K

P

G

G

V

V

P

P

I

K

D

K

F

T

A

T

K

A

H

N

T

T

L

L

L

T

A

T

E

P

F

Y

N

N

D

D

L

L

D

E

A

A

V

V

E

K

D

A

I

L

F

F

K

A

K

E

Y

N

G

P

N

G

S

E

I

I

A

A

A

G

V

V

I

I

V

L

E
|
E

P

P

V

I

A

V

G

G

N
|
N

M

S

G

G

V

F

I

I

R

V

P

P

V

D

E

A

G

G

F

F

L

L

E

E

G

G

L

L

R

R

R

E

V

I

C

T

D

L

D

E

Y

H

G

D

A

A

I

L

L

L

I

V

F

F

D
|
D

E

E

V
|
V

M
|
M

T

T

G

G

F

F

R

R

L

I

T

A

Y

Y

G

G

G

G

A

V

Q

Q

H

E

I

K

Y

F

N

G

V

V

K

T

P

P

D

D

I

L

T

T

I

T

L

L

G

G

K
|
K

V

I

V

I

G

G

G

G

G

G

F

L

P

P

A

V

A

G

C

A

F

Y

G

G

A

G

R

K

K

R

E

E

I

I

M

M

E

Q

Y

L

L

V

A

A

P

P

E

A

G

G

E

P

V

M

Y

Y

Q

Q

A

A

G
|
G

T
|
T

L

L

S

S

G

G

N

N

P

P

I

L

A

A

M

M

A

T

A

A

G

G

I

I

A

K

T

T

L

L

D

E

I

L

L

L

K

R

R

Q

K

P

N

G

P

T

Y

Y

E

E

E

Y

L

L

N

D

K

Q

N

I

T

T

Q

K

K

R

I

L

I

S

S

D

R

G

I

L

I

L

G

A

L

I

G

A

V

Q

E

E

Y

T

G

G

I

H

P

A

V

A

S

C

G

G

E

G

A

Q

I

V

G

S

S

G

M

M

F

F

S

G

I

F

F

F

F

F

K

T

E

E

D

G

K

P

P

V

K

H

N

N

Y

Y

S

E

E

D

V

A

S

K

K

K

S

S

D

D

Q

L

R

Q

L

K

F

F

T

S

N

R

Y

F

F

H

I

R

G

G

L

M

L

L

E

E

R

Q

G

G

I

I

Y

Y

I

L

P

A

P

P

S

S

Q

Q

F

F

E
|
E

S
x
A

N

G

F

F

I

T

T

S

I

L

K

A

H

H

S

T

L

E

A

E

D

D

I

I

E

D

N

A

T

T

L

L

V

A

A

A

V

A

E

R

E

T

V

V

F

M

R

S

E

A

L

L

active site: V21 (= V20), Y144 (= Y143), D239 (= D238), M242 (= M241), K267 (= K266), A401 (≠ S400)
binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: S23 (= S22), V25 (= V24), W61 (= W60), G117 (= G116), T118 (= T117), Y144 (= Y143), H145 (= H144), E206 (= E205), N211 (= N210), D239 (= D238), V241 (= V240), M242 (= M241), K267 (= K266), G298 (= G297), T299 (= T298), E400 (= E399)

2hp2A Inter-subunit signaling in gsam (see paper)
49% identity, 98% coverage: 4:426/433 of query aligns to 5:427/427 of 2hp2A

query
sites
2hp2A

K

K

S

S

R

D

R

E

L

I

F

F

E

A

E

A

A

A

K

Q

R

K

Y

L

I

M

V

P

G

G

G

G

V
|
V

N

S

S
|
S

P

P

V

V

R

R

A

A

F

F

K

K

N

S

V

V

G

G

R

G

T

Q

P

P

L

I

F

V

I

F

A

D

K

R

A

V

K

K

G

D

S

A

K

Y

I

A

Y

W

D

D

E

V

D

D

G

G

N

N

E

R

F

Y

I

I

D

D

Y

Y

V

V

N

G

S

T

W

W

G

G

P

P

A

A

I

I

L

C

G

G

H

H

A

A

D

H

D

P

E

E

V

V

I

I

D

E

K

A

I

L

E

K

E

V

V

A

L

M

E

E

K

K

G

G

F

T

S

S

F

F

G

G

A

A

P

P

T

C

I

A

L

L

E

E

T

N

E

V

I

L

A

A

K

E

R

M

I

V

V

N

N

D

A

A

F

V

D

P

S

S

I

I

E

E

L

M

V

V

R

R

F

F

V

V

S

N

S

S

G
|
G

T
|
T

E

E

A

A

A

C

M

M

S

A

A

V

I

L

R

R

L

I

A

M

R

R

G

A

V

Y

T

T

G

G

R

R

D

D

N

K

I

I

L

I

K

K

F

F

T

E

G

G

C

C

Y
|
Y

H
|
H

G

G

H

H

S

A

D

D

S

M

L

F

L

L

V

V

G

K

A

A

G

G

S

S

G

G

S

V

S

A

T

T

F

L

G

G

V

L

P

P

S

S

S

S

K

P

G

G

V

V

P

P

I

K

D

K

F

T

A

T

K

A

H

N

T

T

L

L

L

T

A

T

E

P

F

Y

N

N

D

D

L

L

D

E

A

A

V

V

E

K

D

A

I

L

F

F

K

A

K

E

Y

N

G

P

N

G

S

E

I

I

A

A

A

G

V

V

I

I

V

L

E
|
E

P

P

V

I

A

V

G

G

N
|
N

M

S

G

G

V

F

I

I

R

V

P

P

V

D

E

A

G

G

F

F

L

L

E

E

G

G

L

L

R

R

R

E

V

I

C

T

D

L

D

E

Y

H

G

D

A

A

I

L

L

L

I

V

F

F

D
|
D

E

E

V
|
V

M
|
M

T

T

G

G

F

F

R

R

L

I

T

A

Y

Y

G

G

G

G

A

V

Q

Q

H

E

I

K

Y

F

N

G

V

V

K

T

P

P

D

D

I

L

T

T

I

T

L

L

G

G

K
|
K

V

I

V

I

G

G

G

G

G

G

F

L

P

P

A

V

A

G

C

A

F

Y

G

G

A

G

R

K

K

R

E

E

I

I

M

M

E

Q

Y

L

L

V

A

A

P

P

E

A

G

G

E

P

V

M

Y

Y

Q

Q

A

A

G
|
G

T
|
T

L

L

S

S

G

G

N

N

P

P

I

L

A

A

M

M

A

T

A

A

G

G

I

I

A

K

T

T

L

L

D

E

I

L

L

L

K

R

R

Q

K

P

N

G

P

T

Y

Y

E

E

E

Y

L

L

N

D

K

Q

N

I

T

T

Q

K

K

R

I

L

I

S

S

D

R

G

I

L

I

L

G

A

L

I

G

A

V

Q

E

E

Y

T

G

G

I

H

P

A

V

A

S

C

G

G

E

G

A

Q

I

V

G

S

S

G

M

M

F

F

S

G

I

F

F

F

F

F

K

T

E

E

D

G

K

P

P

V

K

H

N

N

Y

Y

S

E

E

D

V

A

S

K

K

K

S

S

D

D

Q

L

R

Q

L

K

F

F

T

S

N

R

Y

F

F

H

I

R

G

G

L

M

L

L

E

E

R

Q

G

G

I

I

Y

Y

I

L

P

A

P

P

S

S

Q

Q

F

F

E

E

S
x
A

N

G

F

F

I

T

T

S

I

L

K

A

H

H

S

T

L

E

A

E

D

D

I

I

E

D

N

A

T

T

L

L

V

A

A

A

V

A

E

R

E

T

V

V

F

M

R

S

E

A

L

L

active site: V21 (= V20), Y144 (= Y143), D239 (= D238), M242 (= M241), K267 (= K266), A401 (≠ S400)
binding (4s)-4,5-diaminopentanoic acid: G298 (= G297), T299 (= T298)
binding (4r)-5-amino-4-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]pentanoic acid: S23 (= S22), G117 (= G116), T118 (= T117), Y144 (= Y143), H145 (= H144), E206 (= E205), N211 (= N210), D239 (= D238), V241 (= V240), M242 (= M241), K267 (= K266)
binding pyridoxal-5'-phosphate: G298 (= G297), T299 (= T298)

2hp1A Inter-subunit signaling in gsam (see paper)
49% identity, 98% coverage: 4:426/433 of query aligns to 5:427/427 of 2hp1A

query
sites
2hp1A

K

K

S

S

R

D

R

E

L

I

F

F

E

A

E

A

A

A

K

Q

R

K

Y

L

I

M

V

P

G

G

G

G

V
|
V

N

S

S
|
S

P

P

V
|
V

R

R

A

A

F

F

K

K

N

S

V

V

G

G

R

G

T

Q

P

P

L

I

F

V

I

F

A

D

K

R

A

V

K

K

G

D

S

A

K

Y

I

A

Y

W

D

D

E

V

D

D

G

G

N

N

E

R

F

Y

I

I

D

D

Y

Y

V

V

N

G

S

T

W
|
W

G

G

P

P

A

A

I

I

L

C

G

G

H

H

A

A

D

H

D

P

E

E

V

V

I

I

D

E

K

A

I

L

E

K

E

V

V

A

L

M

E

E

K

K

G

G

F

T

S

S

F

F

G

G

A

A

P

P

T

C

I

A

L

L

E

E

T

N

E

V

I

L

A

A

K

E

R

M

I

V

V

N

N

D

A

A

F

V

D

P

S

S

I

I

E

E

L

M

V

V

R

R

F

F

V

V

S

N

S
|
S

G
|
G

T
|
T

E

E

A

A

A

C

M

M

S

A

A

V

I

L

R

R

L

I

A

M

R

R

G

A

V

Y

T

T

G

G

R

R

D

D

N

K

I

I

L

I

K

K

F

F

T

E

G

G

C

C

Y
|
Y

H
|
H

G
|
G

H

H

S

A

D

D

S

M

L

F

L

L

V

V

G

K

A

A

G

G

S

S

G

G

S

V

S

A

T

T

F

L

G

G

V

L

P

P

S

S

S

S

K

P

G

G

V

V

P

P

I

K

D

K

F

T

A

T

K

A

H

N

T

T

L

L

L

T

A

T

E

P

F

Y

N

N

D

D

L

L

D

E

A

A

V

V

E

K

D

A

I

L

F

F

K

A

K

E

Y

N

G

P

N

G

S

E

I

I

A

A

A

G

V

V

I

I

V

L

E
|
E

P

P

V

I

A

V

G

G

N
|
N

M

S

G

G

V

F

I

I

R

V

P

P

V

D

E

A

G

G

F

F

L

L

E

E

G

G

L

L

R

R

R

E

V

I

C

T

D

L

D

E

Y

H

G

D

A

A

I

L

L

L

I

V

F

F

D
|
D

E

E

V
|
V

M
|
M

T

T

G

G

F

F

R

R

L

I

T

A

Y

Y

G

G

G

G

A

V

Q

Q

H

E

I

K

Y

F

N

G

V

V

K

T

P

P

D

D

I

L

T

T

I

T

L

L

G

G

K
|
K

V

I

V

I

G

G

G

G

G

G

F

L

P

P

A

V

A

G

C

A

F

Y

G

G

A

G

R

K

K

R

E

E

I

I

M

M

E

Q

Y

L

L

V

A

A

P

P

E

A

G

G

E

P

V

M

Y

Y

Q

Q

A

A

G

G

T

T

L

L

S

S

G

G

N

N

P

P

I

L

A

A

M

M

A

T

A

A

G

G

I

I

A

K

T

T

L

L

D

E

I

L

L

L

K

R

R

Q

K

P

N

G

P

T

Y

Y

E

E

E

Y

L

L

N

D

K

Q

N

I

T

T

Q

K

K

R

I

L

I

S

S

D

R

G

I

L

I

L

G

A

L

I

G

A

V

Q

E

E

Y

T

G

G

I

H

P

A

V

A

S

C

G

G

E

G

A

Q

I

V

G

S

S

G

M

M

F

F

S

G

I

F

F

F

F

F

K

T

E

E

D

G

K

P

P

V

K

H

N

N

Y

Y

S

E

E

D

V

A

S

K

K

K

S

S

D

D

Q

L

R

Q

L

K

F

F

T

S

N

R

Y

F

F

H

I

R

G

G

L

M

L

L

E

E

R

Q

G

G

I

I

Y

Y

I

L

P

A

P

P

S

S

Q

Q

F

F

E
|
E

S
x
A

N

G

F

F

I

T

T

S

I

L

K

A

H

H

S

T

L

E

A

E

D

D

I

I

E

D

N

A

T

T

L

L

V

A

A

A

V

A

E

R

E

T

V

V

F

M

R

S

E

A

L

L

active site: V21 (= V20), Y144 (= Y143), D239 (= D238), M242 (= M241), K267 (= K266), A401 (≠ S400)
binding (4s)-4-amino-5-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]pentanoic acid: S23 (= S22), V25 (= V24), W61 (= W60), S116 (= S115), G117 (= G116), T118 (= T117), Y144 (= Y143), H145 (= H144), G146 (= G145), E206 (= E205), N211 (= N210), D239 (= D238), V241 (= V240), M242 (= M241), K267 (= K266), E400 (= E399)

2hozA Inter-subunit signaling in gsam (see paper)
49% identity, 98% coverage: 4:426/433 of query aligns to 5:427/427 of 2hozA

query
sites
2hozA

K

K

S

S

R

D

R

E

L

I

F

F

E

A

E

A

A

A

K

Q

R

K

Y

L

I

M

V

P

G

G

G

G

V
|
V

N

S

S

S

P

P

V

V

R

R

A

A

F

F

K

K

N

S

V

V

G

G

R

G

T

Q

P

P

L

I

F

V

I

F

A

D

K

R

A

V

K

K

G

D

S

A

K

Y

I

A

Y

W

D

D

E

V

D

D

G

G

N

N

E

R

F

Y

I

I

D

D

Y

Y

V

V

N

G

S

T

W

W

G

G

P

P

A

A

I

I

L

C

G

G

H

H

A

A

D

H

D

P

E

E

V

V

I

I

D

E

K

A

I

L

E

K

E

V

V

A

L

M

E

E

K

K

G

G

F

T

S

S

F

F

G

G

A

A

P

P

T

C

I

A

L

L

E

E

T

N

E

V

I

L

A

A

K

E

R

M

I

V

V

N

N

D

A

A

F

V

D

P

S

S

I

I

E

E

L

M

V

V

R

R

F

F

V

V

S

N

S

S

G
|
G

T
|
T

E

E

A

A

A

C

M

M

S

A

A

V

I

L

R

R

L

I

A

M

R

R

G

A

V

Y

T

T

G

G

R

R

D

D

N

K

I

I

L

I

K

K

F

F

T
x
E

G

G

C

C

Y
|
Y

H
|
H

G
|
G

H

H

S

A

D

D

S

M

L

F

L

L

V

V

G

K

A

A

G
|
G

S
|
S

G

G

S

V

S

A

T

T

F

L

G

G

V

L

P

P

S

S

S

S

K

P

G

G

V

V

P

P

I

K

D

K

F

T

A

T

K

A

H

N

T

T

L

L

L

T

A

T

E
x
P

F

Y

N

N

D

D

L

L

D

E

A

A

V

V

E

K

D

A

I

L

F

F

K

A

K

E

Y

N

G

P

N

G

S

E

I

I

A

A

A

G

V

V

I

I

V

L

E
|
E

P

P

V

I

A

V

G

G

N
|
N

M

S

G

G

V

F

I

I

R

V

P

P

V

D

E

A

G

G

F

F

L

L

E

E

G

G

L

L

R

R

R

E

V

I

C

T

D

L

D

E

Y

H

G

D

A

A

I

L

L

L

I

V

F

F

D
|
D

E

E

V

V

M
|
M

T

T

G

G

F

F

R

R

L

I

T

A

Y

Y

G

G

G

G

A

V

Q

Q

H

E

I

K

Y

F

N

G

V

V

K

T

P

P

D

D

I

L

T

T

I

T

L

L

G

G

K
|
K

V

I

V

I

G

G

G

G

G

G

F

L

P

P

A

V

A

G

C

A

F

Y

G

G

A

G

R

K

K

R

E

E

I

I

M

M

E

Q

Y

L

L

V

A

A

P

P

E

A

G

G

E

P

V

M

Y

Y

Q

Q

A

A

G
|
G

T
|
T

L

L

S

S

G

G

N

N

P

P

I

L

A

A

M

M

A

T

A

A

G

G

I

I

A

K

T

T

L

L

D

E

I

L

L

L

K

R

R

Q

K

P

N

G

P

T

Y

Y

E

E

E

Y

L

L

N

D

K

Q

N

I

T

T

Q

K

K

R

I

L

I

S

S

D

R

G

I

L

I

L

G

A

L

I

G

A

V

Q

E

E

Y

T

G

G

I

H

P

A

V

A

S

C

G

G

E

G

A

Q

I

V

G

S

S

G

M

M

F

F

S

G

I

F

F

F

F

F

K

T

E

E

D

G

K

P

P

V

K

H

N
|
N

Y

Y

S
x
E

E

D

V

A

S
x
K

K

K

S

S

D

D

Q

L

R

Q

L

K

F

F

T

S

N

R

Y

F

F

H

I

R

G

G

L

M

L

L

E

E

R

Q

G

G

I

I

Y

Y

I

L

P

A

P

P

S

S

Q

Q

F

F

E

E

S
x
A

N

G

F

F

I

T

T

S

I

L

K

A

H

H

S

T

L

E

A

E

D

D

I

I

E

D

N

A

T

T

L

L

V

A

A

A

V

A

E

R

E

T

V

V

F

M

R

S

E

A

L

L

active site: V21 (= V20), Y144 (= Y143), D239 (= D238), M242 (= M241), K267 (= K266), A401 (≠ S400)
binding (4s)-4,5-diaminopentanoic acid: E141 (≠ T140), G156 (= G155), S157 (= S156), P182 (≠ E181), N368 (= N367), E370 (≠ S369), K373 (≠ S372)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G117 (= G116), T118 (= T117), Y144 (= Y143), H145 (= H144), G146 (= G145), E206 (= E205), N211 (= N210), D239 (= D238), G298 (= G297), T299 (= T298)

3fqaA Gabaculien complex of gabaculine resistant gsam version (see paper)
49% identity, 98% coverage: 4:426/433 of query aligns to 4:426/426 of 3fqaA

query
sites
3fqaA

K

K

S

S

R

D

R

E

L

I

F

F

E

A

E

A

A

A

K

Q

R

K

Y

L

I

M

V

P

G

G

G

G

V
|
V

N

S

S
|
S

P

P

V

V

R
|
R

A

A

F

F

K

K

N

S

V

V

G

G

R

G

T

Q

P

P

L

I

F

V

I

F

A

D

K

R

A

V

K

K

G

D

S

A

K

Y

I

A

Y

W

D

D

E

V

D

D

G

G

N

N

E

R

F

Y

I

I

D

D

Y

Y

V

V

N

G

S

T

W
|
W

G

G

P

P

A

A

I

I

L

C

G

G

H

H

A

A

D

H

D

P

E

E

V

V

I

I

D

E

K

A

I

L

E

K

E

V

V

A

L

M

E

E

K

K

G

G

F

T

S

S

F

F

G

G

A

A

P

P

T

C

I

A

L

L

E

E

T

N

E

V

I

L

A

A

K

E

R

M

I

V

V

N

N

D

A

A

F

V

D

P

S

S

I

I

E

E

L

M

V

V

R

R

F

F

V

V

S

N

S

S

G
|
G

T
|
T

E

E

A

A

A

C

M

M

S

A

A

V

I

L

R

R

L

L

A

M

R

R

G

A

V

Y

T

T

G

G

R

R

D

D

N

K

I

I

L

I

K

K

F

F

T

E

G

G

C

C

Y
|
Y

H

H

G

G

H

H

S

A

D

D

S

M

L

F

L

L

V

V

G

K

A

A

G

G

S

S

G

G

S

V

S

A

T

T

F

L

G

G

V

L

P

P

S

S

S

S

K

P

G

G

V

V

P

P

I

K

D

K

F

T

A

T

K

A

H

N

T

T

L

L

L

T

A

T

E

P

F

Y

N

N

D

D

L

L

D

E

A

A

V

V

E

K

D

A

I

L

F

F

K

A

K

E

Y

N

G

P

N

G

S

E

I

I

A

A

A

G

V

V

I

I

V

L

E
|
E

P

P

V

I

A

V

G

G

N
|
N

M

S

G

G

V

F

I

I

R

V

P

P

V

D

E

A

G

G

F

F

L

L

E

E

G

G

L

L

R

R

R

E

V

I

C

T

D

L

D

E

Y

H

G

D

A

A

I

L

L

L

I

V

F

F

D
|
D

E

E

V
|
V

M
x
I

T

T

G

G

F

F

R

R

L

I

T

A

Y

Y

G

G

G

G

A

V

Q

Q

H

E

I

K

Y

F

N

G

V

V

K

T

P

P

D

D

I

L

T

T

I

T

L

L

G

G

K
|
K

V

I

V

I

G

G

G

G

G

G

F

L

P

P

A

V

A

G

C

A

F

Y

G

G

A

G

R

K

K

R

E

E

I

I

M

M

E

Q

Y

L

L

V

A

A

P

P

E

A

G

G

E

P

V

M

Y

Y

Q

Q

A

A

G

G

T

T

L

L

S

S

G

G

N

N

P

P

I

L

A

A

M

M

A

T

A

A

G

G

I

I

A

K

T

T

L

L

D

E

I

L

L

L

K

R

R

Q

K

P

N

G

P

T

Y

Y

E

E

E

Y

L

L

N

D

K

Q

N

I

T

T

Q

K

K

R

I

L

I

S

S

D

R

G

I

L

I

L

G

A

L

I

G

A

V

Q

E

E

Y

T

G

G

I

H

P

A

V

A

S

C

G

G

E

G

A

Q

I

V

G

S

S

G

M

M

F

F

S

G

I

F

F

F

F

F

K

T

E

E

D

G

K

P

P

V

K

H

N

N

Y

Y

S

E

E

D

V

A

S

K

K

K

S

S

D

D

Q

L

R

Q

L

K

F

F

T

S

N

R

Y

F

F

H

I

R

G

G

L

M

L

L

E

E

R

Q

G

G

I

I

Y

Y

I

L

P

A

P

P

S

S

Q

Q

F

F

E

E

S
x
A

N

G

F

F

I

T

T

S

I

L

K

A

H

H

S

T

L

E

A

E

D

D

I

I

E

D

N

A

T

T

L

L

V

A

A

A

V

A

E

R

E

T

V

V

F

M

R

S

E

A

L

L

active site: V20 (= V20), Y143 (= Y143), D238 (= D238), I241 (≠ M241), K266 (= K266), A400 (≠ S400)
binding 3-aminobenzoic acid: S22 (= S22), R25 (= R25), W60 (= W60)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G116 (= G116), T117 (= T117), Y143 (= Y143), E205 (= E205), N210 (= N210), D238 (= D238), V240 (= V240), I241 (≠ M241)

6w80A Crystal structure of glutamate-1-semialdehyde 2,1-aminomutase from stenotrophomonas maltophilia k279a in complex with plp
46% identity, 96% coverage: 3:416/433 of query aligns to 9:419/430 of 6w80A

query
sites
6w80A

E

D

K

Q

S

S

R

H

R

A

L

L

F

F

E

S

E

R

A

A

K

Q

R

Q

Y

L

I

L

V

P

G

G

G

G

V
|
V

N

N

S

S

P

P

V

V

R

R

A

A

F

F

K

K

N

S

V

V

G

G

R

G

T

E

P

P

L

F

F

F

I

V

A

E

K

R

A

A

K

D

G

G

S

A

K

Y

I

L

Y

Y

D

D

E

V

D

D

G

G

N

N

E

R

F

Y

I

I

D

D

Y

Y

V

V

N

G

S

S

W

W

G

G

P

P

A

M

I

I

L

V

G

G

H

H

A

N

D

H

D

P

E

A

V

V

I

R

D

Q

K

A

I

V

E

K

E

K

V

A

L

I

E

D

K

N

G

G

F

L

S

S

F

F

G

G

A

A

P

P

T

C

I

A

L

G

E

E

T

V

E

T

I

M

A

A

K

E

R

T

I

I

V

T

N

R

A

L

F

V

D

P

S

S

I

C

E

E

L

M

V

V

R

R

F

M

V

V

S

N

S
|
S

G
|
G

T
|
T

E

E

A

A

A

T

M

L

S

S

A

A

I

I

R

R

L

L

A

A

R

R

G

G

V

A

T

T

G

G

R

R

D

N

N

R

I

I

L

V

K

K

F

F

T

E

G

G

C

C

Y
|
Y

H
|
H

G

G

H

H

S

G

D

D

S

S

L

F

L

L

V

V

G

K

A

A

G

-

S

-

G

-

S

M

S

L

T

T

F

L

G

G

V

V

P

P

S

T

S

S

K

P

G

G

V

V

P

P

I

A

D

G

F

L

A

S

K

E

H

L

T

T

L

L

L

T

A

L

E

P

F

Y

N

N

D

D

L

F

D

E

A

A

V

A

E

T

D

A

I

L

F

F

K

E

K

Q

Y

Q

G

G

N

D

S

D

I

I

A

A

A

G

V

L

I

I

V

I

E
|
E

P

P

V

V

A

V

G

G

N
|
N

M

A

G

N

V

C

I

I

R

P

P

P

V

R

E

E

G

G

F

Y

L

L

E

Q

G

H

L

L

R

R

R

A

V

L

C

C

D

T

D

K

Y

H

G

G

A

A

I

L

L

L

I

I

F

F

D
|
D

E

E

V
|
V

M

M

T

T

G

G

F

F

R

R

L

V

T

A

Y

L

G

G

G

G

A

A

Q

Q

H

A

I

H

Y

Y

N

G

V

I

K

T

P

P

D

D

I

L

T

T

I

T

L

F

G

G

K
|
K

V

I

V

I

G

G

G

G

G

G

F

M

P

P

A

V

A

G

C

A

F

Y

G

G

A

G

R

R

K

R

E

E

I

L

M

M

E

Q

Y

Q

L

I

A

A

P

P

E

A

G

G

E

P

V

I

Y

Y

Q

Q

A

A

G

G

T

T

L

L

S

S

G

G

N

N

P

P

I

V

A

A

M

M

A

A

A

A

G

G

I

L

A

A

T

M

L

L

D

E

I

L

L

V

K

Q

R

Q

K

P

N

G

P

F

Y

H

E

A

E

D

L

L

N

A

K

E

N

R

T

T

Q

A

K

R

I

L

I

C

S

A

R

G

I

L

I

E

G

A

L

A

G

A

V

A

E

D

Y

A

G

G

I

V

P

A

V

V

S

T

G

T

E

T

A

R

I

V

G

G

S

A

M

M

F

F

S

G

I

L

F

F

F

F

K

T

E

S

D

E

K

K

P

V

K

E

N

T

Y

Y

S

A

E

Q

V

A

S

T

K

A

S

C

D

D

Q

I

R

P

L

A

F

F

T

N

N

R

Y

F

F

F

I

H

G

A

L

M

L

L

E

E

R

Q

G

G

I

V

Y

F

I

L

P

A

P

P

S

S

Q

A

F

Y

E

E

S

A

N

G

F

F

I

L

T

S

I

S

K

A

H

H

S

D

L

D

A

A

D

V

I

I

E

E

N

A

T

T

L

L

active site: V26 (= V20), Y149 (= Y143), D241 (= D238), K269 (= K266)
binding pyridoxal-5'-phosphate: S121 (= S115), G122 (= G116), T123 (= T117), Y149 (= Y143), H150 (= H144), E208 (= E205), N213 (= N210), D241 (= D238), V243 (= V240), K269 (= K266)

3usfB Crystal structure of dava-4
46% identity, 98% coverage: 4:426/433 of query aligns to 5:402/402 of 3usfB

query
sites
3usfB

K

K

S

S

R

D

R

E

L

I

F

F

E

A

E

A

A

A

K

Q

R

K

Y

L

I

M

V

P

G

G

G

G

V
|
V

N

S

S

S

P

P

V

V

R

R

A

A

F

F

K

K

N

S

V

V

G

G

R

G

T

Q

P

P

L

I

F

V

I

F

A

D

K

R

A

V

K

K

G

D

S

A

K

Y

I

A

Y

W

D

D

E

V

D

D

G

G

N

N

E

R

F

Y

I

I

D

D

Y

Y

V

V

N

G

S

T

W

W

G

G

P

P

A

A

I

I

L

C

G

G

H

H

A

A

D

H

D

P

E

E

V

V

I

I

D

E

K

A

I

L

E

K

E

V

V

A

L

M

E

E

K

K

G

G

F

T

S

S

F

F

G

G

A

A

P

P

T

C

I

A

L

L

E

E

T

N

E

V

I

L

A

A

K

E

R

M

I

V

V

N

N

D

A

A

F

V

D

P

S

S

I

I

E

E

L

M

V

V

R

R

F

F

V

V

S

N

S

S

G
|
G

T
|
T

E

E

A

A

A

C

M

M

S

A

A

V

I

L

R

R

L

I

A

M

R

R

G

A

V

Y

T

T

G

G

R

R

D

D

N

K

I

I

L

I

K

K

F

F

T

E

G

G

C

C

Y
|
Y

H

H

G

G

H

H

S

A

D

D

S

T

L

-

L

-

V

-

G

-

A

-

G

-

S

-

G

-

S

-

S

-

T

-

F

-

G

-

V

-

P

-

S

-

S

-

K

-

G

-

V

-

P

-

I

-

D

-

F

-

A

-

K

A

H

N

T

T

L

L

L

T

A

T

E

P

F

Y

N

N

D

D

L

L

D

E

A

A

V

V

E

K

D

A

I

L

F

F

K

A

K

E

Y

N

G

P

N

G

S

E

I

I

A

A

A

G

V

V

I

I

V

L

E
|
E

P

P

V

I

A

V

G

G

N
|
N

M

S

G

G

V

F

I

I

R

V

P

P

V

D

E

A

G

G

F

F

L

L

E

E

G

G

L

L

R

R

R

E

V

I

C

T

D

L

D

E

Y

H

G

D

A

A

I

L

L

L

I

V

F

F

D
|
D

E

E

V
|
V

M
|
M

T

T

G

G

F

F

R

R

L

I

T

A

Y

Y

G

G

G

G

A

V

Q

Q

H

E

I

K

Y

F

N

G

V

V

K

T

P

P

D

D

I

L

T

T

I

T

L

L

G

G

K
|
K

V

I

V

I

G

G

G

G

G

G

F

L

P

P

A

V

A

G

C

A

F

Y

G

G

A

G

R

K

K

R

E

E

I

I

M

M

E

Q

Y

L

L

V

A

A

P

P

E

A

G

G

E

P

V

M

Y

Y

Q

Q

A

A

G
|
G

T
|
T

L

L

S

S

G

G

N

N

P

P

I

L

A

A

M

M

A

T

A

A

G

G

I

I

A

K

T

T

L

L

D

E

I

L

L

L

K

R

R

Q

K

P

N

G

P

T

Y

Y

E

E

E

Y

L

L

N

D

K

Q

N

I

T

T

Q

K

K

R

I

L

I

S

S

D

R

G

I

L

I

L

G

A

L

I

G

A

V

Q

E

E

Y

T

G

G

I

H

P

A

V

A

S

C

G

G

E

G

A

Q

I

V

G

S

S

G

M

M

F

F

S

G

I

F

F

F

F

F

K

T

E

E

D

G

K

P

P

V

K

H

N

N

Y

Y

S

E

E

D

V

A

S

K

K

K

S

S

D

D

Q

L

R

Q

L

K

F

F

T

S

N

R

Y

F

F

H

I

R

G

G

L

M

L

L

E

E

R

Q

G

G

I

I

Y

Y

I

L

P

A

P

P

S

S

Q

Q

F

F

E

E

S
x
A

N

G

F

F

I

T

T

S

I

L

K

A

H

H

S

T

L

E

A

E

D

D

I

I

E

D

N

A

T

T

L

L

V

A

A

A

V

A

E

R

E

T

V

V

F

M

R

S

E

A

L

L

active site: V21 (= V20), Y144 (= Y143), E181 (= E205), D214 (= D238), M217 (= M241), K242 (= K266), A376 (≠ S400)
binding (4s)-4,5-diaminopentanoic acid: G273 (= G297), T274 (= T298)
binding (5-hydroxy-4,6-dimethylpyridin-3-yl)methyl dihydrogen phosphate: G117 (= G116), T118 (= T117), Y144 (= Y143), E181 (= E205), N186 (= N210), D214 (= D238), V216 (= V240), M217 (= M241), K242 (= K266)

2hp1B Inter-subunit signaling in gsam (see paper)
45% identity, 98% coverage: 4:426/433 of query aligns to 5:398/398 of 2hp1B

query
sites
2hp1B

K

K

S

S

R

D

R

E

L

I

F

F

E

A

E

A

A

A

K

Q

R

K

Y

L

I

M

V

P

G

G

G

G

V
|
V

N

S

S
|
S

P

P

V
|
V

R
|
R

A

A

F

F

K

K

N

S

V

V

G

G

R

G

T

Q

P

P

L

I

F

V

I

F

A

D

K

R

A

V

K

K

G

D

S

A

K

Y

I

A

Y

W

D

D

E

V

D

D

G

G

N

N

E

R

F

Y

I

I

D

D

Y

Y

V

V

N

G

S

T

W
|
W

G

G

P

P

A

A

I

I

L

C

G

G

H

H

A

A

D

H

D

P

E

E

V

V

I

I

D

E

K

A

I

L

E

K

E

V

V

A

L

M

E

E

K

K

G

G

F

T

S

S

F

F

G

G

A

A

P

P

T

C

I

A

L

L

E

E

T

N

E

V

I

L

A

A

K

E

R

M

I

V

V

N

N

D

A

A

F

V

D

P

S

S

I

I

E

E

L

M

V

V

R

R

F

F

V

V

S

N

S
|
S

G
|
G

T
|
T

E

E

A

A

A

C

M

M

S

A

A

V

I

L

R

R

L

I

A

M

R

R

G

A

V

Y

T

T

G

G

R

R

D

D

N

K

I

I

L

I

K

K

F

F

T

E

G

G

C

C

Y
|
Y

H

H

G

G

H

H

S

N

D

-

S

-

L

-

L

-

V

-

G

-

A

-

G

-

S

-

G

-

S

-

S

-

T

-

F

-

G

-

V

-

P

-

S

-

S

-

K

-

G

-

V

-

P

-

I

-

D

-

F

-

A

-

K

-

H

-

T

T

L

L

L

T

A

T

E

P

F

Y

N

N

D

D

L

L

D

E

A

A

V

V

E

K

D

A

I

L

F

F

K

A

K

E

Y

N

G

P

N

G

S

E

I

I

A

A

A

G

V

V

I

I

V

L

E
|
E

P

P

V

I

A

V

G

G

N
|
N

M

S

G

G

V

F

I

I

R

V

P

P

V

D

E

A

G

G

F

F

L

L

E

E

G

G

L

L

R

R

R

E

V

I

C

T

D

L

D

E

Y

H

G

D

A

A

I

L

L

L

I

V

F

F

D
|
D

E

E

V
|
V

M
|
M

T

T

G

G

F

F

R

R

L

I

T

A

Y

Y

G

G

G

G

A

V

Q

Q

H

E

I

K

Y

F

N

G

V

V

K

T

P

P

D

D

I

L

T

T

I

T

L

L

G

G

K
|
K

V

I

V

I

G

G

G

G

G

G

F

L

P

P

A

V

A

G

C

A

F

Y

G

G

A

G

R

K

K

R

E

E

I

I

M

M

E

Q

Y

L

L

V

A

A

P

P

E

A

G

G

E

P

V

M

Y

Y

Q

Q

A

A

G
|
G

T
|
T

L

L

S

S

G

G

N

N

P

P

I

L

A

A

M

M

A

T

A

A

G

G

I

I

A

K

T

T

L

L

D

E

I

L

L

L

K

R

R

Q

K

P

N

G

P

T

Y

Y

E

E

E

Y

L

L

N

D

K

Q

N

I

T

T

Q

K

K

R

I

L

I

S

S

D

R

G

I

L

I

L

G

A

L

I

G

A

V

Q

E

E

Y

T

G

G

I

H

P

A

V

A

S

C

G

G

E

G

A

Q

I

V

G

S

S

G

M

M

F

F

S

G

I

F

F

F

F

F

K

T

E

E

D

G

K

P

P

V

K

H

N

N

Y

Y

S

E

E

D

V

A

S

K

K

K

S

S

D

D

Q

L

R

Q

L

K

F

F

T

S

N

R

Y

F

F

H

I

R

G

G

L

M

L

L

E

E

R

Q

G

G

I

I

Y

Y

I

L

P

A

P

P

S

S

Q

Q

F

F

E

E

S
x
A

N

G

F

F

I

T

T

S

I

L

K

A

H

H

S

T

L

E

A

E

D

D

I

I

E

D

N

A

T

T

L

L

V

A

A

A

V

A

E

R

E

T

V

V

F

M

R

S

E

A

L

L

active site: V21 (= V20), Y144 (= Y143), E177 (= E205), D210 (= D238), M213 (= M241), K238 (= K266), A372 (≠ S400)
binding (4s)-4-amino-5-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]pentanoic acid: G269 (= G297), T270 (= T298)
binding (4s)-4,5-diaminopentanoic acid: S23 (= S22), V25 (= V24), R26 (= R25), W61 (= W60), Y144 (= Y143)
binding pyridoxal-5'-phosphate: S116 (= S115), G117 (= G116), T118 (= T117), Y144 (= Y143), E177 (= E205), N182 (= N210), D210 (= D238), V212 (= V240), M213 (= M241), K238 (= K266)

Query Sequence

>WP_022669880.1 NCBI__GCF_000420385.1:WP_022669880.1
MFEKSRRLFEEAKRYIVGGVNSPVRAFKNVGRTPLFIAKAKGSKIYDEDGNEFIDYVNSW
GPAILGHADDEVIDKIEEVLEKGFSFGAPTILETEIAKRIVNAFDSIELVRFVSSGTEAA
MSAIRLARGVTGRDNILKFTGCYHGHSDSLLVGAGSGSSTFGVPSSKGVPIDFAKHTLLA
EFNDLDAVEDIFKKYGNSIAAVIVEPVAGNMGVIRPVEGFLEGLRRVCDDYGAILIFDEV
MTGFRLTYGGAQHIYNVKPDITILGKVVGGGFPAACFGARKEIMEYLAPEGEVYQAGTLS
GNPIAMAAGIATLDILKRKNPYEELNKNTQKIISRIIGLGVEYGIPVSGEAIGSMFSIFF
KEDKPKNYSEVSKSDQRLFTNYFIGLLERGIYIPPSQFESNFITIKHSLADIENTLVAVE
EVFRELSSGRFYV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory