SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_022670427.1 NCBI__GCF_000420385.1:WP_022670427.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1j32A Aspartate aminotransferase from phormidium lapideum
44% identity, 99% coverage: 5:399/400 of query aligns to 2:387/388 of 1j32A

query
sites
1j32A

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I

active site: W124 (= W127), D202 (= D205), I204 (= I207), K237 (= K240)
binding pyridoxal-5'-phosphate: G98 (= G101), G99 (≠ A102), K100 (= K103), W124 (= W127), N174 (= N177), D202 (= D205), I204 (= I207), Y205 (= Y208), K237 (= K240), R245 (= R248)

P58350 Aspartate aminotransferase; AAT; AspAT; Putative 2-aminoadipate transaminase; Transaminase A; EC 2.6.1.1; EC 2.6.1.39 from Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) (see paper)
45% identity, 98% coverage: 9:399/400 of query aligns to 17:409/410 of P58350

query
sites
P58350

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K249 (= K240) modified: N6-(pyridoxal phosphate)lysine

6f35A Crystal structure of the aspartate aminotranferase from rhizobium meliloti (see paper)
45% identity, 98% coverage: 9:399/400 of query aligns to 7:399/400 of 6f35A

query
sites
6f35A

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binding pyridoxal-5'-phosphate: G99 (= G101), A100 (= A102), K101 (= K103), D204 (= D205), M206 (≠ I207), Y207 (= Y208), S238 (= S239), K239 (= K240), R247 (= R248)

Q02635 Aspartate/prephenate aminotransferase; AspAT / PAT; Transaminase A; EC 2.6.1.1; EC 2.6.1.79 from Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) (see paper)
45% identity, 97% coverage: 13:400/400 of query aligns to 11:400/400 of Q02635

query
sites
Q02635

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x
K

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A

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G

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F

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D

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D

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N

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A

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K

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I

I

N

P

E

E

L

L

R

R

D

E

A

A

V

I

V

A

E

K

K

K

E

F

K

K

N

R

K

E

N

N

G

N

L

L

E

D

Y

Y

K

T

R

A

E

A

N

Q

V

T

C

I

I

V

S

G

V

T

G

G

A

G

K

K

H

Q

A

I

L

L

F

F

N

N

I

A

A

F

A

M

V

A

M

T

L

L

E

N

E

P

G

G

D

D

E

E

V

V

I

V

I

I

I

P

A

A

P

P

Y

Y

W

W

V

V

T

S

Y

Y

E

P

A

E

I

M

V

V

S

A

Y

L

V

C

G

G

G

G

K

T

A

P

V

V

I

F

V

V

N

P

T

T

T

R

E

Q

E

E

N

N

G

N

F

F

V

K

P

L

T

K

K

A

E

E

Q

D

L

L

E

D

K

R

A

A

I

I

T

T

P

P

K

K

T

T

K

K

M

W

I

F

W

V

V

F

N

N

N

S

P

P

T

S

N

N

P

P

T

S

G

G

A

A

T

A

Y

Y

T

S

V

H

D

E

D

E

L

L

K

K

F

A

I

L

V

T

E

D

L

V

A

L

E

M

K

K

N

H

-

P

D

H

I

V

W

W

L

V

V

L

S

T

D

D

E

D

I

M

Y

Y

E

E

D

H

I

L

V

T

F

Y

D

G

G

D

Y

F

K

R

P

F

V

A

S

T

M

P

A

V

T

E

L

V

S

E

D

P

Y

G

A

L

Y

Y

E

E

R

R

T

T

L

L

V

T

V

M

N

N

G

G

V

V

S

S

K

K

T

A

Y

Y

S

A

M

M

T

T

G

G

W

W

R

R

I

I

G

G

Y

Y

T

A

C

A

G

G

D

P

A

L

E

H

V

L

I

I

G

K

A

A

M

M

I

D

K

M

L

I

Q

Q

S

G

Q

Q

S

Q

T

T

S

S

N

G

P

A

T

A

S

S

I

I

A

A

Q

Q

C

W

A

A

A

A

L

V

E

E

A

A

L

L

T

N

G

G

D

P

Q

Q

D

D

S

F

V

I

E

G

K

R

M

N

R

K

V

E

Q

I

F

F

E

Q

K

G

R

R

R

R

D

D

Y

L

I

V

V

V

D

S

A

M

L

L

N

N

S

Q

I

A

E

K

G

G

I

I

S

S

C

C

F

P

K

T

P

P

K

E

G

G

A

A

F

F

Y

Y

V

V

F

Y

P

P

N

S

I

C

S

A

S

G

F

L

F

I

G

G

K

K

E

T

Y

A

-

P

E

S

G

G

K

K

K

V

I

I

N

E

G

T

S

D

M

E

D

D

F

F

A

V

E

S

L

E

L

L

L

L

E

E

H

T

H

E

H

G

V

V

A

A

V

V

V

V

P

H

G

G

I

S

A

A

F

F

G

G

D

L

D

G

R

P

F

N

L

F

R

R

M

I

S

S

F

Y

A

A

T

T

S

S

L

E

E

A

D

L

I

L

Q

E

E

E

G

A

I

C

K

R

R

R

L

I

K

Q

E

R

F

F

V

C

E

A

K

A

I

C

K

R

K12 (≠ Q14) Important for prephenate aminotransferase activity; mutation to G: 7-fold increase in Km for prephenate. Weakly affects Km for oxaloacetate.

6f77A Crystal structure of the prephenate aminotransferase from rhizobium meliloti (see paper)
45% identity, 97% coverage: 13:400/400 of query aligns to 10:399/399 of 6f77A

query
sites
6f77A

I

V

Q

K

P

P

S

S

M

A

T

T

I

I

G

A

I

V

S

S

A

Q

K

K

A

A

K

R

E

E

L

L

R

K

A

A

A

K

G

G

V

R

N

D

V

V

I

I

N

G

F

L

S

G

A

A

G

G

E

E

P

P

D

D

F

F

D

D

T

T

P

P

D

D

N

N

I

I

K

K

M

K

A

A

A

A

V

I

K

D

S

A

I

I

A

D

D

R

G

G

F

E

T

T

K

K

Y

Y

T

T

A

P

A

V

G

S

G

G

I

I

N

P

E

E

L

L

R

R

D

E

A

A

V

I

V

A

E

K

K

K

E

F

K

K

N

R

K

E

N

N

G

N

L

L

E

D

Y

Y

K

T

R

A

E

A

N

Q

V

T

C

I

I

V

S

G

V

T

G
|
G

A
x
G

K
|
K

H

Q

A

I

L

L

F

F

N

N

I

A

A

F

A

M

V

A

M

T

L

L

E

N

E

P

G

G

D

D

E

E

V

V

I

V

I

I

I

P

A

A

P

P

Y

Y

W
|
W

V

V

T

S

Y

Y

E

P

A

E

I

M

V

V

S

A

Y

L

V

C

G

G

G

G

K

T

A

P

V

V

I

F

V

V

N

P

T

T

T

R

E

Q

E

E

N

N

G

N

F

F

V

K

P

L

T

K

K

A

E

E

Q

D

L

L

E

D

K

R

A

A

I

I

T

T

P

P

K

K

T

T

K

K

M

W

I

F

W

V

V

F

N
|
N

N

S

P

P

T

S

N
|
N

P

P

T

S

G

G

A

A

T

A

Y

Y

T

S

V

H

D

E

D

E

L

L

K

K

F

A

I

L

V

T

E

D

L

V

A

L

E

M

K

K

N

H

-

P

D

H

I

V

W

W

L

V

V

L

S

T

D
|
D

E

D

I

M

Y
|
Y

E

E

D

H

I

L

V

T

F

Y

D

G

G

D

Y

F

K

R

P

F

V

A

S

T

M

P

A

V

T

E

L

V

S

E

D

P

Y

G

A

L

Y

Y

E

E

R

R

T

T

L

L

V

T

V

M

N

N

G

G

V

V

S
|
S

K
|
K

T

A

Y

Y

S

A

M

M

T

T

G

G

W

W

R
|
R

I

I

G

G

Y

Y

T

A

C

A

G

G

D

P

A

L

E

H

V

L

I

I

G

K

A

A

M

M

I

D

K

M

L

I

Q

Q

S

G

Q

Q

S

Q

T

T

S

S

N

G

P

A

T

A

S

S

I

I

A

A

Q

Q

C

W

A

A

A

A

L

V

E

E

A

A

L

L

T

N

G

G

D

P

Q

Q

D

D

S

F

V

I

E

G

K

R

M

N

R

K

V

E

Q

I

F

F

E

Q

K

G

R

R

R

R

D

D

Y

L

I

V

V

V

D

S

A

M

L

L

N

N

S

Q

I

A

E

K

G

G

I

I

S

S

C

C

F

P

K

T

P

P

K

E

G

G

A

A

F

F

Y

Y

V

V

F

Y

P

P

N

S

I

C

S

A

S

G

F

L

F

I

G

G

K

K

E

T

Y

A

-

P

E

S

G

G

K

K

K

V

I

I

N

E

G

T

S

D

M

E

D

D

F

F

A

V

E

S

L

E

L

L

L

L

E

E

H

T

H

E

H

G

V

V

A

A

V

V

V

V

P

H

G

G

I

S

A

A

F

F

G

G

D

L

D

G

R

P

F

N

L

F

R

R

M

I

S

S

F

Y

A

A

T

T

S

S

L

E

E

A

D

L

I

L

Q

E

E

E

G

A

I

C

K

R

R

R

L

I

K

Q

E

R

F

F

V

C

E

A

K

A

I

C

K

R

binding pyridoxal-5'-phosphate: G98 (= G101), G99 (≠ A102), K100 (= K103), W124 (= W127), N170 (= N173), N174 (= N177), D203 (= D205), Y206 (= Y208), S237 (= S239), K238 (= K240), R246 (= R248)

1bkgA Aspartate aminotransferase from thermus thermophilus with maleate (see paper)
45% identity, 97% coverage: 6:392/400 of query aligns to 4:378/382 of 1bkgA

query
sites
1bkgA

L

L

N

S

R

R

R

R

I

V

G

Q

L

A

I

M

Q

K

P

P

S

S

M

A

T

T

I

V

G

A

I

V

S

N

A

A

K

K

A

A

K

L

E

E

L

L

R

R

A

R

A

Q

G

G

V

V

N

D

V

L

I

V

N

A

F

L

S

T

A

A

G

G

E

E

P

P

D

D

F

F

D

D

T

T

P

P

D

E

N

H

I

V

K

K

M

E

A

A

A

A

V

R

K

R

S

A

I

L

A

A

D

Q

G

G

F

K

T

T

K

K

Y

Y

T

A

A

P

A

P

G

A

G

G

I

I

N

P

E

E

L

L

R

R

D

E

A

A

V

L

V

A

E

E

K

K

E

F

K

R

N

R

K

E

N

N

G

G

L

L

E

S

Y

V

K

T

R

P

E

E

N

E

V

T

C

I

I

V

S

T

V

V

G
|
G

A
x
G

K
|
K

H

Q

A

A

L

L

F

F

N

N

I

L

A

F

A

Q

V

A

M

I

L

L

E

D

E

P

G

G

D

D

E

E

V

V

I

I

I

V

I

L

A

S

P

P

Y

Y

W
|
W

V

V

T

S

Y

Y

E

P

A

E

I

M

V

V

S

R

Y

F

V

A

G

G

G

G

K

V

A

V

V

V

I

E

V

V

N

E

T

T

T

L

E

P

E

E

N

E

G

G

F

F

V

V

P

P

T

D

K

P

E

E

Q

R

L

V

E

R

K

R

A

A

I

I

T

T

P

P

K

R

T

T

K

K

M

A

I

L

W

V

V

V

N
|
N

N

S

P

P

T

N

N
|
N

P

P

T

T

G

G

A

A

T

V

Y

Y

T

P

V

K

D

E

D

V

L

L

K

E

F

A

I

L

V

A

E

R

L

L

A

A

E

V

K

E

N

H

D

D

I

F

W

Y

L

L

V

V

S

S

D
|
D

E

E

I
|
I

Y
|
Y

E

E

D

H

I

L

V

L

F

Y

D

E

G

G

-

E

-

H

Y

F

K

S

P

P

V

G

S

R

M

V

A

A

T

P

L

-

S

-

D

-

Y

-

A

-

Y

-

E

E

R

H

T

T

L

L

V

T

V

V

N

N

G

G

V

A

S

A

K
|
K

T

A

Y

F

S

A

M

M

T

T

G

G

W

W

R
|
R

I

I

G

G

Y

Y

T

A

C

C

G

G

D

P

A

K

E

E

V

V

I

I

G

K

A

A

M

M

I

A

K

S

L

V

Q

S

S

S

Q

Q

S

S

T

T

S

T

N

S

P

P

T

D

S

T

I

I

A

A

Q

Q

C

W

A

A

A

T

L

L

E

E

A

A

L

L

T

T

G

N

D

Q

Q

E

D

A

S

S

-

R

-

A

-

F

V

V

E

E

K

M

M

A

R

R

V

E

Q

A

F

Y

E

R

K

R

R

R

R

R

D

D

Y

L

I

L

V

L

D

E

A

G

L

L

N

T

S

A

I

L

E

-

G

G

I

L

S

K

C

A

F

V

K

R

P

P

K

S

G

G

A

A

F

F

Y

Y

V

V

F

L

P

M

N

D

I

T

S

S

S

P

F

I

F

A

G

P

K

D

E

E

Y

V

E

R

G

A

K

-

K

-

I

-

N

-

G

-

S

-

M

-

D

-

F

-

A

A

E

E

L

R

L

L

L

L

E

E

H

-

H

A

H

G

V

V

A

A

V

V

V

V

P

P

G

G

I

T

A

D

F

F

G

A

D

A

D

F

R

G

F

H

L

V

R

R

M

L

S

S

F

Y

A

A

T

T

S

S

L

E

E

E

D

N

I

L

Q

R

E

K

G

A

I

L

K

E

R

R

L

F

active site: W125 (= W127), D203 (= D205), I205 (= I207), K234 (= K240)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G99 (= G101), G100 (≠ A102), K101 (= K103), W125 (= W127), N171 (= N173), N175 (= N177), D203 (= D205), I205 (= I207), Y206 (= Y208), K234 (= K240), R242 (= R248)

1bjwA Aspartate aminotransferase from thermus thermophilus (see paper)
45% identity, 97% coverage: 6:392/400 of query aligns to 4:378/382 of 1bjwA

query
sites
1bjwA

L

L

N

S

R

R

R

R

I

V

G

Q

L

A

I

M

Q

K

P

P

S

S

M

A

T

T

I

V

G

A

I

V

S

N

A

A

K

K

A

A

K

L

E

E

L

L

R

R

A

R

A

Q

G

G

V

V

N

D

V

L

I

V

N

A

F

L

S

T

A

A

G
|
G

E

E

P

P

D

D

F

F

D

D

T

T

P

P

D

E

N

H

I

V

K

K

M

E

A

A

A

A

V

R

K

R

S

A

I

L

A

A

D

Q

G

G

F

K

T

T

K

K

Y

Y

T

A

A

P

A

P

G

A

G

G

I

I

N

P

E

E

L

L

R

R

D

E

A

A

V

L

V

A

E

E

K

K

E

F

K

R

N

R

K

E

N

N

G

G

L

L

E

S

Y

V

K

T

R

P

E

E

N

E

V

T

C

I

I

V

S

T

V

V

G

G

A

G

K

K

H

Q

A

A

L

L

F

F

N

N

I

L

A

F

A

Q

V

A

M

I

L

L

E

D

E

P

G

G

D

D

E

E

V

V

I

I

I

V

I

L

A

S

P

P

Y

Y

W
|
W

V

V

T

S

Y

Y

E

P

A

E

I

M

V

V

S

R

Y

F

V

A

G

G

G

G

K

V

A

V

V

V

I

E

V

V

N

E

T

T

T

L

E

P

E

E

N

E

G

G

F

F

V

V

P

P

T

D

K

P

E

E

Q

R

L

V

E

R

K

R

A

A

I

I

T

T

P

P

K

R

T

T

K

K

M

A

I

L

W

V

V

V

N

N

N

S

P

P

T

N

N
|
N

P

P

T

T

G

G

A

A

T

V

Y

Y

T

P

V

K

D

E

D

V

L

L

K

E

F

A

I

L

V

A

E

R

L

L

A

A

E

V

K

E

N

H

D

D

I

F

W

Y

L

L

V

V

S

S

D
|
D

E

E

I
|
I

Y

Y

E

E

D

H

I

L

V

L

F

Y

D

E

G

G

-

E

-

H

Y

F

K

S

P

P

V

G

S

R

M

V

A

A

T

P

L

-

S

-

D

-

Y

-

A

-

Y

-

E

E

R

H

T

T

L

L

V

T

V

V

N

N

G

G

V

A

S

A

K
|
K

T

A

Y

F

S

A

M

M

T

T

G

G

W

W

R

R

I

I

G

G

Y

Y

T

A

C

C

G

G

D

P

A

K

E

E

V

V

I

I

G

K

A

A

M

M

I

A

K

S

L

V

Q

S

S

S

Q

Q

S

S

T

T

S

T

N

S

P

P

T

D

S

T

I

I

A

A

Q

Q

C

W

A

A

A

T

L

L

E

E

A

A

L

L

T

T

G

N

D

Q

Q

E

D

A

S

S

-

R

-

A

-

F

V

V

E

E

K

M

M

A

R

R

V

E

Q

A

F

Y

E

R

K

R

R

R

R

R

D

D

Y

L

I

L

V

L

D

E

A

G

L

L

N

T

S

A

I

L

E

-

G

G

I

L

S

K

C

A

F

V

K

R

P

P

K

S

G

G

A

A

F

F

Y

Y

V

V

F

L

P

M

N

D

I

T

S

S

S

P

F

I

F

A

G

P

K

D

E

E

Y

V

E

R

G

A

K

-

K

-

I

-

N

-

G

-

S

-

M

-

D

-

F

-

A

A

E

E

L

R

L

L

L

L

E

E

H

-

H

A

H

G

V

V

A

A

V

V

V

V

P

P

G

G

I

T

A

D

F

F

G

A

D

A

D

F

R

G

F

H

L

V

R
|
R

M

L

S

S

F

Y

A

A

T

T

S

S

L

E

E

E

D

N

I

L

Q

R

E

K

G

A

I

L

K

E

R

R

L

F

active site: W125 (= W127), D203 (= D205), I205 (= I207), K234 (= K240)
binding phosphate ion: G39 (= G41), W125 (= W127), N175 (= N177), R361 (= R375)

Q56232 Aspartate/prephenate aminotransferase; AspAT / PAT; Transaminase A; EC 2.6.1.1; EC 2.6.1.78 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see 3 papers)
45% identity, 97% coverage: 6:392/400 of query aligns to 4:378/385 of Q56232

query
sites
Q56232

L

L

N

S

R

R

R

R

I

V

G

Q

L

A

I

M

Q
x
K

P

P

S

S

M

A

T

T

I

V

G

A

I

V

S

N

A

A

K

K

A

A

K

L

E

E

L

L

R

R

A

R

A

Q

G

G

V

V

N

D

V

L

I

V

N

A

F

L

S

T

A

A

G

G

E

E

P

P

D

D

F

F

D

D

T

T

P

P

D

E

N

H

I

V

K

K

M

E

A

A

A

A

V

R

K

R

S

A

I

L

A

A

D

Q

G

G

F

K

T

T

K

K

Y

Y

T

A

A

P

A

P

G

A

G

G

I

I

N

P

E

E

L

L

R

R

D

E

A

A

V

L

V

A

E

E

K

K

E

F

K

R

N

R

K

E

N

N

G

G

L

L

E

S

Y

V

K

T

R

P

E

E

N

E

V

T

C

I

I

V

S

T

V

V

G

G

A

G

K

K

H

Q

A

A

L

L

F

F

N

N

I

L

A

F

A

Q

V

A

M

I

L

L

E

D

E

P

G

G

D

D

E

E

V

V

I

I

I

V

I

L

A

S

P

P

Y

Y

W

W

V

V

T

S

Y

Y

E

P

A

E

I

M

V

V

S

R

Y

F

V

A

G

G

G

G

K

V

A

V

V

V

I

E

V

V

N

E

T

T

T

L

E

P

E

E

N

E

G

G

F

F

V

V

P

P

T

D

K

P

E

E

Q

R

L

V

E

R

K

R

A

A

I

I

T

T

P

P

K

R

T

T

K

K

M

A

I

L

W

V

V

V

N

N

N

S

P

P

T

N

N

N

P

P

T

T

G

G

A

A

T

V

Y

Y

T

P

V

K

D

E

D

V

L

L

K

E

F

A

I

L

V

A

E

R

L

L

A

A

E

V

K

E

N

H

D

D

I

F

W

Y

L

L

V

V

S

S

D

D

E

E

I

I

Y

Y

E

E

D

H

I

L

V

L

F

Y

D

E

G

G

-

E

-

H

Y

F

K

S

P

P

V

G

S

R

M

V

A

A

T

P

L

-

S

-

D

-

Y

-

A

-

Y

-

E

E

R

H

T

T

L

L

V

T

V

V

N

N

G

G

V

A

S

A

K
|
K

T

A

Y

F

S

A

M

M

T

T

G

G

W

W

R

R

I

I

G

G

Y

Y

T

A

C

C

G

G

D

P

A

K

E

E

V

V

I

I

G

K

A

A

M

M

I

A

K

S

L

V

Q

S

S

S

Q

Q

S

S

T

T

S

T

N

S

P

P

T

D

S

T

I

I

A

A

Q

Q

C

W

A

A

A

T

L

L

E

E

A

A

L

L

T

T

G

N

D

Q

Q

E

D

A

S

S

-

R

-

A

-

F

V

V

E

E

K

M

M

A

R

R

V

E

Q

A

F

Y

E

R

K

R

R

R

R

R

D

D

Y

L

I

L

V

L

D

E

A

G

L

L

N

T

S

A

I

L

E

-

G

G

I

L

S

K

C

A

F

V

K

R

P

P

K

S

G

G

A

A

F

F

Y

Y

V

V

F

L

P

M

N

D

I

T

S

S

S

P

F

I

F

A

G

P

K

D

E

E

Y

V

E

R

G

A

K

-

K

-

I

-

N

-

G

-

S

-

M

-

D

-

F

-

A

A

E

E

L

R

L

L

L

L

E

E

H

-

H

A

H

G

V

V

A

A

V

V

V

V

P

P

G

G

I

T

A

D

F

F

G

A

D

A

D

F

R

G

F

H

L

V

R

R

M

L

S

S

F

Y

A

A

T

T

S

S

L

E

E

E

D

N

I

L

Q

R

E

K

G

A

I

L

K

E

R

R

L

F

K12 (≠ Q14) Important for prephenate aminotransferase activity; mutation to G: 10-fold increase in Km for prephenate. Does not affect Km for oxaloacetate.
K234 (= K240) modified: N6-(pyridoxal phosphate)lysine

1b5oA Thermus thermophilus aspartate aminotransferase single mutant 1 (see paper)
44% identity, 97% coverage: 6:392/400 of query aligns to 4:378/382 of 1b5oA

query
sites
1b5oA

L

L

N

S

R

R

R

R

I

V

G

Q

L

A

I

M

Q

K

P

P

S

S

M

A

T

T

I

V

G

A

I

V

S

N

A

A

K

K

A

A

K

L

E

E

L

L

R

R

A

R

A

Q

G

G

V

V

N

D

V

L

I

V

N

A

F

L

S

T

A

A

G

G

E

E

P

P

D

D

F

F

D

D

T

T

P

P

D

E

N

H

I

V

K

K

M

E

A

A

A

A

V

R

K

R

S

A

I

L

A

A

D

Q

G

G

F

K

T

T

K

K

Y

Y

T

A

A

P

A

P

G

A

G

G

I

I

N

P

E

E

L

L

R

R

D

E

A

A

V

L

V

A

E

E

K

K

E

F

K

R

N

R

K

E

N

N

G

G

L

L

E

S

Y

V

K

T

R

P

E

E

N

E

V

T

C

I

I

V

S

T

V

V

G
|
G

A
x
G

K
x
S

H

Q

A

A

L

L

F

F

N

N

I

L

A

F

A

Q

V

A

M

I

L

L

E

D

E

P

G

G

D

D

E

E

V

V

I

I

I

V

I

L

A

S

P

P

Y

Y

W
|
W

V

V

T

S

Y

Y

E

P

A

E

I

M

V

V

S

R

Y

F

V

A

G

G

G

G

K

V

A

V

V

V

I

E

V

V

N

E

T

T

T

L

E

P

E

E

N

E

G

G

F

F

V

V

P

P

T

D

K

P

E

E

Q

R

L

V

E

R

K

R

A

A

I

I

T

T

P

P

K

R

T

T

K

K

M

A

I

L

W

V

V

V

N

N

N

S

P

P

T

N

N
|
N

P

P

T

T

G

G

A

A

T

V

Y

Y

T

P

V

K

D

E

D

V

L

L

K

E

F

A

I

L

V

A

E

R

L

L

A

A

E

V

K

E

N

H

D

D

I

F

W

Y

L

L

V

V

S

S

D
|
D

E

E

I
|
I

Y
|
Y

E

E

D

H

I

L

V

L

F

Y

D

E

G

G

-

E

-

H

Y

F

K

S

P

P

V

G

S

R

M

V

A

A

T

P

L

-

S

-

D

-

Y

-

A

-

Y

-

E

E

R

H

T

T

L

L

V

T

V

V

N

N

G

G

V

A

S
x
A

K
|
K

T

A

Y

F

S

A

M

M

T

T

G

G

W

W

R
|
R

I

I

G

G

Y

Y

T

A

C

C

G

G

D

P

A

K

E

E

V

V

I

I

G

K

A

A

M

M

I

A

K

S

L

V

Q

S

S

S

Q

Q

S

S

T

T

S

T

N

S

P

P

T

D

S

T

I

I

A

A

Q

Q

C

W

A

A

A

T

L

L

E

E

A

A

L

L

T

T

G

N

D

Q

Q

E

D

A

S

S

-

R

-

A

-

F

V

V

E

E

K

M

M

A

R

R

V

E

Q

A

F

Y

E

R

K

R

R

R

R

R

D

D

Y

L

I

L

V

L

D

E

A

G

L

L

N

T

S

A

I

L

E

-

G

G

I

L

S

K

C

A

F

V

K

R

P

P

K

S

G

G

A

A

F

F

Y

Y

V

V

F

L

P

M

N

D

I

T

S

S

S

P

F

I

F

A

G

P

K

D

E

E

Y

V

E

R

G

A

K

-

K

-

I

-

N

-

G

-

S

-

M

-

D

-

F

-

A

A

E

E

L

R

L

L

L

L

E

E

H

-

H

A

H

G

V

V

A

A

V

V

V

V

P

P

G

G

I

T

A

D

F

F

G

A

D

A

D

F

R

G

F

H

L

V

R

R

M

L

S

S

F

Y

A

A

T

T

S

S

L

E

E

E

D

N

I

L

Q

R

E

K

G

A

I

L

K

E

R

R

L

F

active site: W125 (= W127), D203 (= D205), I205 (= I207), K234 (= K240)
binding pyridoxal-5'-phosphate: G99 (= G101), G100 (≠ A102), S101 (≠ K103), W125 (= W127), N175 (= N177), D203 (= D205), I205 (= I207), Y206 (= Y208), A233 (≠ S239), K234 (= K240), R242 (= R248)

1gc4A Thermus thermophilus aspartate aminotransferase tetra mutant 2 complexed with aspartate (see paper)
44% identity, 97% coverage: 6:392/400 of query aligns to 4:378/382 of 1gc4A

query
sites
1gc4A

L

L

N

S

R

R

R

R

I

V

G

Q

L

A

I

M

Q

K

P

P

S

D

M

A

T

V

I

V

G

A

I

V

S

N

A

A

K

K

A

A

K

L

E

E

L

L

R

R

A

R

A

Q

G

G

V

V

N

D

V

L

I

V

N

A

F

L

S

T

A

A

G

G

E

E

P

P

D

D

F

F

D

D

T

T

P

P

D

E

N

H

I

V

K

K

M

E

A

A

A

A

V

R

K

R

S

A

I

L

A

A

D

Q

G

G

F

K

T

T

K

K

Y
|
Y

T

A

A

P

A

P

G

A

G

G

I

I

N

P

E

E

L

L

R

R

D

E

A

A

V

L

V

A

E

E

K

K

E

F

K

R

N

R

K

E

N

N

G

G

L

L

E

S

Y

V

K

T

R

P

E

E

N

E

V

T

C

I

I

V

S

T

V

V

G
|
G

A
x
G

K
x
S

H

Q

A

A

L

L

F

F

N

N

I

L

A

F

A

Q

V

A

M

I

L

L

E

D

E

P

G

G

D

D

E

E

V

V

I

I

I

V

I

L

A

S

P

P

Y

Y

W
|
W

V

V

T

S

Y

Y

E

P

A

E

I

M

V

V

S

R

Y

F

V

A

G

G

G

G

K

V

A

V

V

V

I

E

V

V

N

E

T

T

T

L

E

P

E

E

N

E

G

G

F

F

V

V

P

P

T

D

K

P

E

E

Q

R

L

V

E

R

K

R

A

A

I

I

T

T

P

P

K

R

T

T

K

K

M

A

I

L

W

V

V

V

N

N

N

S

P

P

T

N

N
|
N

P

P

T

T

G

G

A

A

T

V

Y

Y

T

P

V

K

D

E

D

V

L

L

K

E

F

A

I

L

V

A

E

R

L

L

A

A

E

V

K

E

N

H

D

D

I

F

W

Y

L

L

V

V

S

S

D
|
D

E

E

I

I

Y
|
Y

E

E

D

H

I

L

V

L

F

Y

D

E

G

G

-

E

-

H

Y

F

K

S

P

P

V

G

S

R

M

V

A

A

T

P

L

-

S

-

D

-

Y

-

A

-

Y

-

E

E

R

H

T

T

L

L

V

T

V

V

N

N

G

G

V

A

S

A

K

K

T

A

Y

F

S

A

M

M

T

T

G

G

W

W

R
|
R

I

I

G

G

Y

Y

T

A

C

C

G

G

D

P

A

K

E

E

V

V

I

I

G

K

A

A

M

M

I

A

K

S

L

V

Q

S

S
x
R

Q

Q

S

S

T

T

S

T

N

S

P

P

T

D

S

T

I

I

A

A

Q

Q

C

W

A

A

A

T

L

L

E

E

A

A

L

L

T

T

G

N

D

Q

Q

E

D

A

S

S

-

R

-

A

-

F

V

V

E

E

K

M

M

A

R

R

V

E

Q

A

F

Y

E

R

K

R

R

R

R

R

D

D

Y

L

I

L

V

L

D

E

A

G

L

L

N

T

S

A

I

L

E

-

G

G

I

L

S

K

C

A

F

V

K

R

P

P

K

S

G

G

A

A

F

F

Y
|
Y

V

V

F

L

P

M

N

D

I

T

S

S

S

P

F

I

F

A

G

P

K

D

E

E

Y

V

E

R

G

A

K

-

K

-

I

-

N

-

G

-

S

-

M

-

D

-

F

-

A

A

E

E

L

R

L

L

L

L

E

E

H

-

H

A

H

G

V

V

A

A

V

V

V

V

P

P

G

G

I

T

A

D

F

F

G

A

D

A

D

F

R

G

F

H

L

V

R
|
R

M

L

S

S

F

Y

A

A

T

T

S

S

L

E

E

E

D

N

I

L

Q

R

E

K

G

A

I

L

K

E

R

R

L

F

binding aspartic acid: Y64 (= Y66), W125 (= W127), N175 (= N177), R261 (≠ S267), Y322 (= Y326), R361 (= R375)
binding pyridoxal-5'-phosphate: G99 (= G101), G100 (≠ A102), S101 (≠ K103), W125 (= W127), N175 (= N177), D203 (= D205), Y206 (= Y208), R242 (= R248)

1gc3A Thermus thermophilus aspartate aminotransferase tetra mutant 2 complexed with tryptophan (see paper)
44% identity, 97% coverage: 6:392/400 of query aligns to 4:378/382 of 1gc3A

query
sites
1gc3A

L

L

N

S

R

R

R

R

I

V

G

Q

L

A

I

M

Q

K

P

P

S

D

M

A

T
x
V

I

V

G

A

I

V

S
x
N

A

A

K

K

A

A

K

L

E

E

L

L

R

R

A

R

A

Q

G

G

V

V

N

D

V

L

I

V

N

A

F

L

S
x
T

A
|
A

G
|
G

E
|
E

P

P

D

D

F

F

D

D

T

T

P

P

D

E

N

H

I

V

K

K

M

E

A

A

A

A

V

R

K

R

S

A

I

L

A

A

D

Q

G

G

F

K

T

T

K

K

Y

Y

T

A

A

P

A

P

G

A

G

G

I

I

N

P

E

E

L

L

R

R

D

E

A

A

V

L

V

A

E

E

K

K

E

F

K

R

N

R

K

E

N

N

G

G

L

L

E

S

Y

V

K

T

R

P

E

E

N

E

V

T

C

I

I

V

S

T

V

V

G

G

A
x
G

K
x
S

H

Q

A

A

L

L

F

F

N

N

I

L

A

F

A

Q

V

A

M

I

L

L

E

D

E

P

G

G

D

D

E

E

V

V

I

I

I

V

I

L

A

S

P

P

Y

Y

W
|
W

V

V

T

S

Y

Y

E

P

A

E

I

M

V

V

S

R

Y

F

V

A

G

G

G

G

K

V

A

V

V

V

I

E

V

V

N

E

T

T

T

L

E

P

E

E

N

E

G

G

F

F

V

V

P

P

T

D

K

P

E

E

Q

R

L

V

E

R

K

R

A

A

I

I

T

T

P

P

K

R

T

T

K

K

M

A

I

L

W

V

V

V

N

N

N

S

P

P

T

N

N
|
N

P

P

T

T

G

G

A

A

T

V

Y

Y

T

P

V

K

D

E

D

V

L

L

K

E

F

A

I

L

V

A

E

R

L

L

A

A

E

V

K

E

N

H

D

D

I

F

W

Y

L

L

V

V

S

S

D
|
D

E

E

I

I

Y
|
Y

E

E

D

H

I

L

V

L

F

Y

D

E

G

G

-

E

-

H

Y

F

K

S

P

P

V

G

S

R

M

V

A

A

T

P

L

-

S

-

D

-

Y

-

A

-

Y

-

E

E

R

H

T

T

L

L

V

T

V

V

N

N

G

G

V

A

S

A

K
|
K

T

A

Y

F

S

A

M

M

T

T

G

G

W

W

R
|
R

I

I

G

G

Y

Y

T

A

C

C

G

G

D

P

A

K

E

E

V

V

I

I

G

K

A

A

M

M

I

A

K

S

L

V

Q

S

S

R

Q

Q

S

S

T

T

S

T

N

S

P

P

T

D

S

T

I

I

A

A

Q

Q

C

W

A

A

A

T

L

L

E

E

A

A

L

L

T

T

G

N

D

Q

Q

E

D

A

S

S

-

R

-

A

-

F

V

V

E

E

K

M

M

A

R

R

V

E

Q

A

F

Y

E

R

K

R

R

R

R

R

D

D

Y

L

I

L

V

L

D

E

A

G

L

L

N

T

S

A

I

L

E

-

G

G

I

L

S

K

C

A

F

V

K

R

P

P

K

S

G

G

A

A

F

F

Y
|
Y

V

V

F

L

P

M

N

D

I

T

S

S

S

P

F

I

F

A

G

P

K

D

E

E

Y

V

E

R

G

A

K

-

K

-

I

-

N

-

G

-

S

-

M

-

D

-

F

-

A

A

E

E

L

R

L

L

L

L

E

E

H

-

H

A

H

G

V

V

A

A

V

V

V

V

P

P

G

G

I

T

A

D

F

F

G

A

D

A

D

F

R

G

F

H

L

V

R
|
R

M

L

S

S

F

Y

A

A

T

T

S

S

L

E

E

E

D

N

I

L

Q

R

E

K

G

A

I

L

K

E

R

R

L

F

binding pyridoxal-5'-phosphate: G100 (≠ A102), S101 (≠ K103), W125 (= W127), N175 (= N177), D203 (= D205), Y206 (= Y208), K234 (= K240), R242 (= R248)
binding tryptophan: V16 (≠ T18), N20 (≠ S22), T37 (≠ S39), A38 (= A40), G39 (= G41), E40 (= E42), Y322 (= Y326), R361 (= R375)

8wkjA The crystal structure of aspartate aminotransferases lpg0070 from legionella pneumophila (see paper)
44% identity, 91% coverage: 32:396/400 of query aligns to 30:390/391 of 8wkjA

query
sites
8wkjA

G

G

V

L

N

D

V

I

I

I

N

G

F

L

S

G

A

T

G

G

E

E

P

P

D

D

F

F

D

D

T

T

P

P

D

Q

N

H

I

I

K

K

M

L

A

A

A

A

V

I

K

S

S

A

I

I

A

E

D

A

G

G

F

D

T

T

K

K

Y

Y

T

T

A

A

A

V

G

D

G

G

I

I

N

V

E

E

L

L

R

K

D

E

A

A

V

V

V

K

E

N

K

K

E

F

K

K

N

R

K

D

N

N

G

E

L

L

E

D

Y

Y

K

Q

R

L

E

N

N

Q

V

I

C

L

I

V

S

S

V

V

G
|
G

A
x
G

K
|
K

H

Q

A

S

L

C

F

Y

N

N

I

L

A

C

A

Q

V

A

M

Y

L

L

E

N

E

P

G

G

D

D

E

E

V

V

I

I

I

I

I

P

A

A

P

P

Y

Y

W
|
W

V

V

T

S

Y

Y

E

P

A

D

I

M

V

V

S

L

Y

L

V

A

G

D

G

G

K

V

A

P

V

V

I

I

V

I

N

E

T

T

T

T

E

P

E

A

N

Q

G

R

F

Y

V

K

P

I

T

N

K

A

E

Q

Q

Q

L

L

E

E

K

Q

A

A

I

I

T

T

P

P

K

K

T

T

K

R

M

M

I

I

W

F

V

L

N
|
N

N

S

P

P

T

S

N
|
N

P

P

T

S

G

G

A

I

T

A

Y

Y

T

T

V

Q

D

N

D

E

L

L

K

K

F

E

I

L

V

G

E

D

L

V

A

L

E

K

K

K

N

H

-

P

D

Q

I

I

W

L

L

I

V

A

S

T

D
|
D

E

D

I

M

Y
|
Y

E

E

D

H

I

I

V

I

F

W

D

S

G

-

Y

-

K

Q

P

P

V

F

S

T

-

N

M

I

A

L

T

N

L

A

S

C

D

P

Y

E

A

L

Y

Y

E

D

R

R

T

T

L

I

V

V

V

L

N

N

G

G

V

V

S
|
S

K
|
K

T

A

Y

Y

S

A

M

M

T

T

G

G

W

W

R
|
R

I

I

G

G

Y

Y

T

A

C

A

G

G

D

P

A

A

E

P

V

L

I

I

G

N

A

A

M

M

I

K

K

T

L

I

Q

Q

S

S

Q

Q

S

S

T

T

S

S

N

N

P

P

T

C

S

S

I

I

A

A

Q

Q

C

R

A

A

A

A

L

V

E

A

A

A

L

L

T

N

G

G

D

S

Q

N

D

E

S

S

V

I

E

E

K

E

M

M

R

V

V

N

Q

A

F

F

E

H

K

Q

R

R

R

H

D

D

Y

Y

I

V

V

A

D

D

A

R

L

L

N