SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_022671020.1 NCBI__GCF_000420385.1:WP_022671020.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1vpeA Crystallographic analysis of phosphoglycerate kinase from the hyperthermophilic bacterium thermotoga maritima (see paper)
52% identity, 99% coverage: 4:394/394 of query aligns to 5:397/398 of 1vpeA

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active site: R35 (= R35), K196 (= K197), G353 (= G350), G376 (= G373)
binding phosphoaminophosphonic acid-adenylate ester: G194 (= G195), A195 (= A196), K196 (= K197), K200 (= K201), G218 (= G219), A219 (= A220), N316 (= N316), P318 (= P318), G320 (= G320), V321 (= V321), E323 (= E323), G352 (= G349), G353 (= G350), D354 (= D351), S355 (≠ T352)

P36204 Bifunctional PGK/TIM; EC 2.7.2.3; EC 5.3.1.1 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
52% identity, 99% coverage: 4:394/394 of query aligns to 6:398/654 of P36204

query
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P36204

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R36 (= R35) binding substrate
R118 (= R117) binding substrate
R151 (= R150) binding substrate

1phpA Structure of the adp complex of the 3-phosphoglycerate kinase from bacillus stearothermophilus at 1.65 angstroms (see paper)
52% identity, 99% coverage: 2:393/394 of query aligns to 4:394/394 of 1phpA

query
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1phpA

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active site: R36 (= R35), K197 (= K197), G351 (= G350), G374 (= G373)
binding adenosine-5'-diphosphate: G195 (= G195), K201 (= K201), G219 (= G219), G220 (≠ A220), L237 (= L237), N316 (= N316), P318 (= P318), G320 (= G320), V321 (= V321), E323 (= E323), G350 (= G349), D352 (= D351), S353 (≠ T352)

P18912 Phosphoglycerate kinase; EC 2.7.2.3 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see paper)
52% identity, 99% coverage: 2:393/394 of query aligns to 4:394/394 of P18912

query
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P18912

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L

P

P

T

T

I

I

Q

R

Y

Y

A

L

I

I

D

E

N

H

K

G

A

A

K

K

V

V

I

I

L

L

A

A

S

S

H

H

L

L

G

G

R

R

P

P

K

K

G

G

K

K

R

V

N

V

P

E

K

E

Y

L

S

R

L

L

K

D

P

A

V

V

A

A

K

K

R

R

L

L

A

G

T

E

I

L

L

L

G

E

K

R

P

P

V

V

L

A

F

K

I

T

E

N

D

E

F

A

E

V

N

G

P

D

E

E

D

V

K

K

A

A

K

A

L

V

E

D

A

R

L

L

K

N

D

E

G

G

D

D

V

V

A

L

L

L

L

L

E

E

N

N

L

V

R

R

F

F

W

Y

E

P

G

G

E

E

E

E

K

K

N

N

S

D

K

P

E

E

F

L

A

A

L

K

K

A

L

F

K

A

E

E

L

L

F

A

D

D

V

L

Y

Y

V

V

N

N

D

D

A

A

F

F

G

G

V

A

C

A

H

H

R

R

K

A

H

H

A

A

S

S

V

T

Y

E

A

G

L

I

A

A

E

H

I

Y

S

L

E

P

I

A

A

V

A

A

I

-

G

G

F

F

L

L

L

M

K

E

K

K

E

E

L

L

E

E

Y

V

F

L

D

G

K

K

I

A

F

L

T

S

I

N

E

P

E

D

K

R

P

P

F

F

V

T

A

A

V

I

I

I

G

G

G

G

A

A

K

K

I

V

S

K

G

D

K
|
K

L

I

D

G

C

V

L

I

L

D

N

N

L

L

L

L

N

E

R

K

A

V

D

D

K

N

I

L

I

I

I

I

G

G

G

G

A

G

Q

L

A

A

F

Y

T

T

F

F

L

V

R

K

A

A

L

L

G

G

Y

H

K

D

T

V

G

G

N

K

S

S

L

L

V

L

E

E

E

E

E

D

L

K

I

I

D

E

E

L

A

A

K

K

R

S

V

F

M

M

D

E

E

K

A

A

G

K

R

E

K

K

G

G

V

V

K

R

L

F

Y

Y

L

M

P

P

V

V

D

D

F

V

V

V

C

V

S

A

T

D

S

R

P

F

E

A

D

N

E

D

S

A

G

N

Y

T

V

K

I

V

K

V

P

P

Y

I

Q

D

E

A

I

I

P

P

K

A

G

D

M

W

M

S

G

A

L

L

D

D

I

I

G

G

P

P

A

K

S

T

I

R

E

E

L

L

F

Y

R

R

E

D

V

V

L

I

S

R

D

E

A

S

K

K

V

L

V

V

V

V

W

W

N
|
N

G

G

P

P

M

M

G

G

V

V

F

F

E
|
E

V

M

K

D

A

A

F

F

A

A

H

H

G

G

T

T

N

K

-

A

R

I

I

A

A

E

Q

A

I

L

I

A

G

E

S

A

L

L

N

D

A

T

L

Y

K

S

V

V

V

I

G

G

G
|
G

G
|
G

D
|
D

T
x
S

A

A

D

A

A

A

V

V

E

E

R

K

A

F

G

G

E

L

S

A

D

D

N

K

M

M

S

D

Y

H

I

I

S

S

T

T

G

G

G

G

G

G

A

A

S

S

L

L

K

E

L

F

L

M

E

E

G

G

K

K

T

Q

L

L

P

P

A

G

V

V

E

V

V

A

L

L

E

E

K

D

K

K

K201 (= K201) binding ATP
N316 (= N316) binding ATP
E323 (= E323) binding ATP
GGDS 350:353 (≠ GGDT 349:352) binding ATP

P40924 Phosphoglycerate kinase; EC 2.7.2.3 from Bacillus subtilis (strain 168) (see paper)
49% identity, 99% coverage: 2:393/394 of query aligns to 4:394/394 of P40924

query
sites
P40924

K

K

V

T

I

L

N

K

E

D

I

I

D

D

I

V

T

K

G

G

K

K

R

V

V

V

F

F

I

C

R

R

C

V

D

D

F

F

N

N

V

V

P

P

M

M

D

-

E

K

N

D

G

G

N

E

I

V

T

T

D

D

D

D

N

T

R

R

I

I

R

R

A

A

A

A

L

L

P

P

T

T

I

I

Q

K

Y

H

A

L

I

A

D

D

N

Q

K

G

A

A

K

K

V

V

I

L

L

L

A

A

S

S

H

H

L

L

G

G

R

R

P

P

K

K

G

G

K

E

R

V

N

V

P

E

K

E

Y

L

S

R

L

L

K

T

P

P

V

V

A

A

K

A

R

R

L

L

A

G

T

E

I

L

L

L

G

G

K

K

P

E

V

V

L

K

F

K

I

A

E

D

D

E

F

A

E

Y

N

G

P

D

E

A

D

V

K

K

A

A

K

Q

L

I

E

S

A

E

L

M

K

K

D

D

G

G

D

D

V

V

A

L

L

V

L

L

E

E

N

N

L

V

R

R

F

F

W

Y

E

P

G

G

E

E

E

E

K

K

N

N

S

D

K

P

E

E

F

L

A

A

L

K

K

A

L

F

K

A

E

E

L

L

F

A

D

D

V

V

Y

Y

V

V

N

N

D

D

A

A

F

F

G

G

V

A

C

A

H

H

R

R

K

A

H

H

A

A

S

S

V

T

Y

A

A

G

L

I

A

A

E

E

I

-

S

H

E

L

I

P

A

A

A

V

I

A

G

G

F

F

L

L

L

M

K

E

K

K

E

E

L

L

E

D

Y

V

F

L

D

G

K

K

I

A

F

V

T
x
S

I

N

E

P

E

D

K

R

P

P

F

F

V

T

A

A

V

I

I

I

G

G

G

G

A

A

K

K

I

V

S

K

G

D

K

K

L

I

D

G

C

V

L

I

L

E

N

S

L

L

L

L

N

D

R

K

A

V

D

D

K

N

I

L

I

I

I

I

G

G

G

G

A

G

Q

L

A

A

F

Y

T

T

F

F

L

V

R

K

A

A

L

L

G

G

Y

Y

K

E

T

V

G

G

N

K

S

S

L

L

V

L

E

E

E

E

E

D

L

K

I

I

D

E

E

L

A

A

K

K

R

S

V

F

M

M

D

D

E

R

A

A

G

K

R

E

K

K

G

G

V

V

K

N

L

F

Y

Y

L

M

P

P

V

E

D

D

F

V

V

L

C

V

S

A

T

D

S

D

P

F

E

S

D

N

E

D

S

A

G

N

Y

V

V

K

I

I

K

V

P

P

Y

I

Q

S

E

E

I

I

P

P

K

S

G

D

M

L

M

E

G

A

L

I

D

D

I

I

G

G

P

T

A

K

S
x
T

I

R

E

E

L

T

F

Y

R

A

E

D

V

V

L

I

S

K

D

N

A

S

K

K

V

L

V

V

V

V

W

W

N

N

G

G

P

P

M

M

G

G

V

V

F

F

E

E

V

I

K

D

A

L

F

F

A

A

H

Q

G

G

T

T

N

K

R

A

I

V

A

A

Q

E

I

A

I

L

G

A

-

E

S

A

L

K

N

D

A

T

L

Y

K

S

V

V

V

I

G

G

G

G

G

G

D

D

T

S

A

A

D

A

A

A

V

V

E

E

R

K

A

F

G

G

E

L

S

A

D

D

N

K

M

M

S

S

Y

H

I

I

S

S

T

T

G

G

G

G

G

G

A

A

S

S

L

L

K

E

L

F

L

M

E

E

G

G

K

K

T

E

L

L

P

P

A

G

V

V

E

A

V

A

L

L

E

N

K

D

K

K

S183 (≠ T183) modified: Phosphoserine
T299 (≠ S299) modified: Phosphothreonine

16pkA Phosphoglycerate kinase from trypanosoma brucei bisubstrate analog (see paper)
44% identity, 99% coverage: 2:393/394 of query aligns to 3:415/415 of 16pkA

query
sites
16pkA

K

K

V

S

I

I

N

N

E

E

I

C

D

D

I

L

T

K

G

G

K

K

R

K

V

V

F

L

I

I

R

R

C

V

D

D

F

F

N

N

V

V

P

P

M

V

D

-

E

K

N

N

G

G

N

K

I

I

T

T

D

N

D

D

N

Y

R
|
R

I

I

R

R

A

S

A

A

L

L

P

P

T

T

I

L

Q

K

Y

K

A

V

I

L

D

T

N

E

K

G

A

G

K

S

V

C

I

V

L

L

A

M

S

S

H

H

L

L

G

G

R

R

P

P

K

K

G

G

-

I

-

P

-

M

-

A

-

Q

-

A

-

G

-

K

-

I

-

R

-

S

-

T

-

G

-

G

-

V

-

P

K

G

R

F

N

Q

P

Q

K

K

Y

A

S

T

L

L

K

K

P

P

V

V

A

A

K

K

R

R

L

L

A

S

T

E

I

L

L

L

G

L

K

R

P

P

V

V

L

T

F

F

I

A

E

P

D

D

F

C

E

L

N

N

P

A

E

A

D

D

K

V

A

-

K

-

L

V

E

S

A

K

L

M

K

S

D

P

G

G

D

D

V

V

A

V

L

L

L

L

E

E

N

N

L

V

R

R

F

F

W

Y

E

K

G

E

E

E

-

G

-

S

-

K

-

K

E

A

K

K

N

D

S

R

K

E

E

A

F

M

A

A

L

K

K

I

L

L

K

A

E

S

L

Y

F

G

D

D

V

V

Y

Y

V

I

N

S

D

D

A

A

F

F

G

G

V

T

C

A

H

H

R

R

K

D

H

S

A

A

S

T

V

M

Y

T

A

G

L

I

A

P

E

K

I

I

S

L

E

G

I

N

A

G

A

A

I

A

G

G

F

Y

L

L

L

M

K

E

K

K

E

E

L

I

E

S

Y

Y

F

F

D

A

K

K

I

V

F

L

T

G

I

N

E

P

E

P

K

R

P

P

F

L

V

V

A

A

V

I

I

V

G

G

G
|
G

A
|
A

K
|
K

I

V

S

S

G

D

K
|
K

L

I

D

Q

C

L

L

L

L

D

N

N

L

M

L

L

N

Q

R

R

A

I

D

D

K

Y

I

L

I

L

I

I

G

G

G

G

A
|
A

Q

M

A

A

F
x
Y

T

T

F

F

L

L

R

K

A

A

L

Q

G

G

Y

Y

K

S

T

I

G

G

N

K

S

S

L

K

V

C

E

E

E

E

E

S

L

K

I

L

D

E

E

F

A

A

K

R

R

S

V

L

M

L

D

K

E

K

A

A

G

E

R

D

K

R

G

K

V

V

K

Q

L

V

Y

I

L

L

P

P

V

I

D

D

F

H

V

V

C

C

S

H

T

T

S

E

P

F

E

K

D

A

E

V

S

D

G

S

Y

P

V

L

I

I

K

T

P

E

Y

D

Q

Q

E

N

I

I

P

P

K

E

G

G

M

H

M

M

G

A

L
|
L

D

D

I

I

G

G

P

P

A

K

S

T

I

I

E

E

L

K

F

Y

R

V

E

Q

V

T

L

I

S

G

D

K

A

C

K

K

V

S

V

A

V

I

W

W

N

N

G

G

P
|
P

M

M

G
|
G

V
|
V

F

F

E
|
E

V

M

K

V

A

P

F

Y

A

S

H

K

G

G

T

T

N

F

R

A

I

I

A

A

Q

K

I

A

I

M

G

G

-

R

-

G

-

T

-

H

S

E

L

H

N

G

A

L

L

M

K

S

V

I

V

I

G

G

G

G

G
|
G

D

D

T

S

A

A

D

S

A

A

V

A

E

E

R

L

A

S

G

G

E

E

S

A

D

K

N

R

M

M

S

S

Y

H

I

V

S

S

T

T

G
|
G

G
|
G

G
|
G

A

A

S

S

L

L

K

E

L

L

L

L

E

E

G

G

K

K

T

T

L

L

P

P

A

G

V

V

E

T

V

V

L

L

E

D

K

D

K

K

active site: R35 (= R35), K215 (= K197), G372 (= G350), G395 (= G373)
binding 1,1,5,5-tetrafluorophosphopentylphosphonic acid adenylate ester: G213 (= G195), A214 (= A196), K219 (= K201), A238 (= A220), Y241 (≠ F223), L311 (= L293), P336 (= P318), G338 (= G320), V339 (= V321), E341 (= E323), G393 (= G371), G394 (= G372), G395 (= G373)

13pkA Ternary complex of phosphoglycerate kinase from trypanosoma brucei (see paper)
44% identity, 99% coverage: 2:393/394 of query aligns to 3:415/415 of 13pkA

query
sites
13pkA

K

K

V

S

I

I

N

N

E

E

I

C

D

D

I

L

T

K

G

G

K

K

R

K

V

V

F

L

I

I

R

R

C

V

D

D

F

F

N

N

V

V

P

P

M

V

D

-

E

K

N

N

G

G

N

K

I

I

T

T

D

N

D

D

N

Y

R
|
R

I

I

R

R

A

S

A

A

L

L

P

P

T

T

I

L

Q

K

Y

K

A

V

I

L

D

T

N

E

K

G

A

G

K

S

V

C

I

V

L

L

A

M

S

S

H

H

L

L

G

G

R

R

P

P

K

K

G

G

-

I

-

P

-

M

-

A

-

Q

-

A

-

G

-

K

-

I

-

R

-

S

-

T

-

G

-

G

-

V

-

P

K

G

R

F

N

Q

P

Q

K

K

Y

A

S

T

L

L

K

K

P

P

V

V

A

A

K

K

R

R

L

L

A

S

T

E

I

L

L

L

G

L

K

R

P

P

V

V

L

T

F

F

I

A

E

P

D

D

F

C

E

L

N

N

P

A

E

A

D

D

K

V

A

-

K

-

L

V

E

S

A

K

L

M

K

S

D

P

G

G

D

D

V

V

A

V

L

L

L

L

E

E

N

N

L

V

R

R

F

F

W

Y

E

K

G

E

E

E

-

G

-

S

-

K

-

K

E

A

K

K

N

D

S

R

K

E

E

A

F

M

A

A

L

K

K

I

L

L

K

A

E

S

L

Y

F

G

D

D

V

V

Y

Y

V

I

N

S

D

D

A

A

F

F

G

G

V

T

C

A

H

H

R

R

K

D

H

S

A

A

S

T

V

M

Y

T

A

G

L

I

A

P

E

K

I

I

S

L

E

G

I

N

A

G

A

A

I

A

G

G

F

Y

L

L

L

M

K

E

K

K

E

E

L

I

E

S

Y

Y

F

F

D

A

K

K

I

V

F

L

T

G

I

N

E

P

E

P

K

R

P

P

F

L

V

V

A

A

V

I

I

V

G

G

G
|
G

A
|
A

K
|
K

I

V

S

S

G

D

K
|
K

L

I

D

Q

C

L

L

L

L

D

N

N

L

M

L

L

N

Q

R

R

A

I

D

D

K

Y

I

L

I

L

I

I

G

G

G

G

A

A

Q

M

A

A

F

Y

T

T

F

F

L

L

R

K

A

A

L

Q

G

G

Y

Y

K

S

T

I

G

G

N

K

S

S

L

K

V

C

E

E

E

E

E

S

L

K

I

L

D

E

E

F

A

A

K

R

R

S

V

L

M

L

D

K

E

K

A

A

G

E

R

D

K

R

G

K

V

V

K

Q

L

V

Y

I

L

L

P

P

V

I

D

D

F

H

V

V

C

C

S

H

T

T

S

E

P

F

E

K

D

A

E

V

S

D

G

S

Y

P

V

L

I

I

K

T

P

E

Y

D

Q

Q

E

N

I

I

P

P

K

E

G

G

M

H

M

M

G

A

L
|
L

D

D

I

I

G

G

P

P

A

K

S

T

I

I

E

E

L

K

F

Y

R

V

E

Q

V

T

L

I

S

G

D

K

A

C

K

K

V

S

V

A

V

I

W

W

N

N

G

G

P
|
P

M

M

G
|
G

V
|
V

F

F

E
|
E

V

M

K

V

A

P

F

Y

A

S

H

K

G

G

T

T

N

F

R

A

I

I

A

A

Q

K

I

A

I

M

G

G

-

R

-

G

-

T

-

H

S

E

L

H

N

G

A

L

L

M

K

S

V

I

V

I

G

G

G
|
G

G
|
G

D
|
D

T
x
S

A

A

D

S

A

A

V

A

E

E

R

L

A

S

G

G

E

E

S

A

D

K

N

R

M

M

S

S

Y

H

I

V

S

S

T

T

G

G

G

G

G
|
G

A

A

S

S

L

L

K

E

L

L

L

L

E

E

G

G

K

K

T

T

L

L

P

P

A

G

V

V

E

T

V

V

L

L

E

D

K

D

K

K

active site: R35 (= R35), K215 (= K197), G372 (= G350), G395 (= G373)
binding adenosine-5'-diphosphate: G213 (= G195), A214 (= A196), K219 (= K201), L311 (= L293), P336 (= P318), G338 (= G320), V339 (= V321), E341 (= E323), G371 (= G349), D373 (= D351), S374 (≠ T352)

P07378 Phosphoglycerate kinase, glycosomal; Phosphoglycerate kinase C; EC 2.7.2.3 from Trypanosoma brucei brucei (see 2 papers)
43% identity, 100% coverage: 2:394/394 of query aligns to 7:420/440 of P07378

query
sites
P07378

K

K

V

S

I

I

N

N

E

E

I

C

D

D

I

L

T

K

G

G

K

K

R

K

V

V

F

L

I

I

R

R

C

V

D
|
D

F
|
F

N
|
N

V

V

P

P

M

V

D

-

E

K

N

N

G

G

N

K

I

I

T

T

D

N

D

D

N

Y

R
|
R

I

I

R

R

A

S

A

A

L

L

P

P

T

T

I

L

Q

K

Y

K

A

V

I

L

D

T

N

E

K

G

A

G

K

S

V

C

I

V

L

L

A

M

S

S

H
|
H

L
|
L

G
|
G

R
|
R

P

P

K

K

G

G

-

I

-

P

-

M

-

A

-

Q

-

A

-

G

-

K

-

I

-

R

-

S

-

T

-

G

-

G

-

V

-

P

K

G

R

F

N

Q

P

Q

K

K

Y

A

S

T

L

L

K

K

P

P

V

V

A

A

K

K

R

A

L

L

A

S

T

E

I

L

L

L

G

L

K

R

P

P

V

V

L

T

F

F

I

A

E

P

D

D

F

C

E

L

N

N

P

A

E

A

D

D

K

V

A

-

K

-

L

V

E

S

A

K

L

M

K

S

D

P

G

G

D

D

V

V

A

V

L

L

L

L

E

E

N

N

L

V

R
|
R

F

F

W

Y

E

K

G

E

E

E

-

G

-

S

-

K

-

K

E

A

K

K

N

D

S

R

K

E

E

A

F

M

A

A

L

K

K

I

L

L

K

A

E

S

L

Y

F

G

D

D

V

V

Y

Y

V

I

N

S

D

D

A

A

F

F

G

G

V

T

C

A

H

H

R
|
R

K

D

H

S

A

A

S

T

V

M

Y

T

A

G

L

I

A

P

E

K

I

I

S

L

E

G

I

N

A

G

A

A

I

A

G

G

F

Y

L

L

L

M

K

E

K

K

E

E

L

I

E

S

Y

Y

F

F

D

A

K

K

I

V

F

L

T

G

I

N

E

P

E

P

K

R

P

P

F

L

V

V

A

A

V

I

I

V

G

G

G

G

A

A

K

K

I

V

S

S

G

D

K
|
K

L

I

D

Q

C

L

L

L

L

D

N

N

L

M

L

L

N

Q

R

R

A

I

D

D

K

Y

I

L

I

L

I

I

G

G

G

G

A

A

Q

M

A

A

F

Y

T

T

F

F

L

L

R

K

A

A

L

Q

G

G

Y

Y

K

S

T

I

G

G

N

K

S

S

L

K

V

C

E

E

E

E

E

S

L

K

I

L

D

E

E

F

A

A

K

R

R

S

V

L

M

L

D

K

E

K

A

A

G

E

R

D

K

R

G

K

V

V

K

Q

L

V

Y

I

L

L

P

P

V

I

D

D

F

H

V

V

C

C

S

H

T

T

S

E

P

F

E

K

D

A

E

V

S

D

G

S

Y

P

V

L

I

I

K

T

P

E

Y

D

Q

Q

E

N

I

I

P

P

K

E

G

G

M

H

M

M

G

A

L

L

D

D

I

I

G

G

P

P

A

K

S

T

I

I

E

E

L

K

F

Y

R

V

E

Q

V

T

L

I

S

G

D

K

A

C

K

K

V

S

V

A

V

I

W

W

N
|
N

G

G

P

P

M

M

G

G

V

V

F

F

E
|
E

V

M

K

V

A

P

F

Y

A

S

H

K

G

G

T

T

N

F

R

A

I

I

A

A

Q

K

I

A

I

M

G

G

-

R

-

G

-

T

-

H

S

E

L

H

N

G

A

L

L

M

K

S

V

I

V

I

G

G

G
|
G

G
|
G

D
|
D

T
x
S

A

A

D

S

A

A

V

A

E

E

R

L

A

S

G

G

E

E

S

A

D

K

N

R

M

M

S

S

Y

H

I

V

S

S

T

T

G

G

G

G

G

G

A

A

S

S

L

L

K

E

L

L

L

L

E

E

G

G

K

K

T

T

L

L

P

P

A

G

V

V

E

T

V

V

L

L

E

D

K

E

K

K

Q

S

DFN 24:26 (= DFN 19:21) binding substrate
R39 (= R35) binding substrate
HLGR 62:65 (= HLGR 58:61) binding substrate
R135 (= R117) binding substrate
R172 (= R150) binding substrate
K223 (= K201) binding ATP
N338 (= N316) binding ATP
E345 (= E323) binding ATP
GGDS 375:378 (≠ GGDT 349:352) binding ATP

P00560 Phosphoglycerate kinase; EC 2.7.2.3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
45% identity, 99% coverage: 4:394/394 of query aligns to 9:416/416 of P00560

query
sites
P00560

I

V

N

Q

E

D

I

L

D

D

I

L

T

K

G

D

K

K

R

R

V

V

F

F

I

I

R
|
R

C

V

D

D

F

F

N

N

V

V

P

P

M

L

D

D

E

-

N

G

G

K

N

K

I

I

T

T

D

S

D

N

N

Q

R
|
R

I

I

R

V

A

A

A

A

L

L

P

P

T

T

I

I

Q

K

Y

Y

A

V

I

L

D

E

N

H

K

H

A

P

K

R

-

Y

V

V

I

V

L

L

A

A

S

S

H

H

L

L

G

G

R

R

P

P

K

N

G

G

K

E

R

R

N

N

P

E

K

K

Y

Y

S

S

L

L

K

A

P

P

V

V

A

A

K

K

R

E

L

L

A

Q

T

S

I

L

L

L

G

G

K

K

P

D

V

V

L

T

F

F

I

L

E

N

D

D

F

C

E

V

N

G

P

P

E

E

D

V

K

E

A

A

K

A

L

V

E

K

A

A

L

S

K

A

D

P

G

G

D

S

V

V

A

I

L

L

L

L

E

E

N

N

L

L

R
|
R

F

Y

W

H

E

I

G

E

E

E

E

E

-

G

-

S

-

R

-

K

-

V

-

D

-

G

-

Q

-

K

-

V

K

K

N

A

S

S

K

K

E

E

-

D

-

V

-

Q

-

K

F

F

A

R

L

H

K

E

L

L

K

S

E

S

L

L

F

A

D

D

V

V

Y

Y

V

I

N

N

D

D

A

A

F

F

G

G

V

T

C

A

H

H

R
|
R

K

A

H

H

A

S

S

S

V

M

Y

V

A

G

L

F

A

-

E

D

I

L

S

P

E

Q

I

R

A

A

A

A

I

-

G

G

F

F

L

L

L

L

K

E

K

K

E

E

L

L

E

K

Y

Y

F

F

D

G

K

K

I

A

F

L

T

E

I

N

E

P

E

T

K

R

P

P

F

F

V

L

A

A

V

I

I

L

G

G

G

G

A

A

K

K

I

V

S

A

G

D

K

K

L

I

D

Q

C

L

L

I

L

D

N

N

L

L

L

L

N

D

R

K

A

V

D

D

K

S

I

I

I

I

I

I

G

G

G

G

A

G

Q

M

A

A

F

F

T

T

F

F

L

K

R

K

A

V

L

L

-

E

G

N

Y

T

K

E

T

I

G

G

N

D

S

S

L

I

V

F

E

D

E

K

E

A

L

G

I

A

D

E

E

I

A

V

K

P

R

K

V

L

M

M

D

E

E

K

A

A

G

K

R

A

K

K

G

G

V

V

K

E

L

V

Y

V

L

L

P

P

V

V

D

D

F

F

V

I

C

I

S

A

T

D

S

A

-

F

P

S

E

A

D

D

E

A

S

N

G

T

Y

K

V

T

I

V

K

T

P

D

Y

K

Q

E

E

G

I

I

P

P

K

A

G

G

M

W

M

Q

G

G

L

L

D

D

I

N

G

G

P

P

A

E

S

S

I

R

E

K

L

L

F

F

R

A

E

A

V

T

L

V

S

A

D

K

A

A

K

K

V

T

V

I

V

V

W

W

N

N

G

G

P

P

M

P

G

G

V

V

F

F

E

E

V

F

K

E

A

K

F

F

A

A

H

A

G

G

T

T

N

K

R

A

I

L

A

L

-

D

Q

E

I

V

I

V

G

K

S

S

L

S

N

A

A

A

-

G

-

N

L

T

K

V

V

I

V

I

G

G

G

G

G

G

D

D

T

T

A

A

D

T

A

V

V

A

E

K

R

K

A

Y

G

G

E

V

S

T

D

D

N

K

M

I

S

S

Y

H

I

V

S

S

T

T

G

G

G

G

G

G

A

A

S

S

L

L

K

E

L

L

L

L

E

E

G

G

K

K

T

E

L

L

P

P

A

G

V

V

E

A

V

F

L

L

E

S

K

E

K

K

Q

K

R22 (= R17) mutation to K: 2-fold reduction of Vmax.; mutation to M: 7-fold reduction of Vmax.
R39 (= R35) binding substrate
R122 (= R117) binding substrate
R169 (= R150) binding substrate

3pgkA The structure of yeast phosphoglycerate kinase at 0.25 nm resolution (see paper)
45% identity, 99% coverage: 4:394/394 of query aligns to 8:415/415 of 3pgkA

query
sites
3pgkA

I

V

N

Q

E

D

I

L

D

D

I

L

T

K

G

D

K

K

R

R

V

V

F

F

I

I

R

R

C

V

D

D

F

F

N

N

V

V

P

P

M

L

D

D

E

-

N

G

G

K

N

K

I

I

T

T

D

S

D

N

N

Q

R
|
R

I

I

R

V

A

A

A

A

L

L

P

P

T

T

I

I

Q

K

Y

Y

A

V

I

L

D

E

N

H

K

H

A

P

K

R

-

Y

V

V

I

V

L

L

A

A

S

S

H

H

L

L

G

G

R

R

P

P

K

N

G

G

K

E

R

R

N

N

P

E

K

K

Y

Y

S

S

L

L

K

A

P

P

V

V

A

A

K

K

R

E

L

L

A

Q

T

S

I

L

L

L

G

G

K

K

P

D

V

V

L

T

F

F

I

L

E

N

D

D

F

C

E

V

N

G

P

P

E

E

D

V

K

E

A

A

K

A

L

V

E

K

A

A

L

S

K

A

D

P

G

G

D

S

V

V

A

I

L

L

L

L

E

E

N

N

L

L

R

R

F

Y

W

H

E

I

G

E

E

E

E

E

-

G

-

S

-

R

-

K

-

V

-

D

-

G

-

Q

-

K

-

V

K

K

N

A

S

S

K

K

E

E

-

D

-

V

-

Q

-

K

F

F

A

R

L

H

K

E

L

L

K

S

E

S

L

L

F

A

D

D

V

V

Y

Y

V

I

N

N

D

D

A

A

F

F

G

G

V

T

C

A

H

H

R

R

K

A

H

H

A

S

S

S

V

M

Y

V

A

G

L

F

A

-

E

D

I

L

S

P

E

Q

I

R

A

A

A

A

I

-

G

G

F

F

L

L

L

L

K

E

K

K

E

E

L

L

E

K

Y

Y

F

F

D

G

K

K

I

A

F

L

T

E

I

N

E

P

E

T

K

R

P

P

F

F

V

L

A

A

V

I

I

L

G

G

G
|
G

A
|
A

K
|
K

I

V

S

A

G

D

K

K

L

I

D

Q

C

L

L

I

L

D

N

N

L

L

L

L

N

D

R

K

A

V

D

D

K

S

I

I

I

I

I

I

G

G

G

G

A

G

Q

M

A

A

F

F

T

T

F

F

L

K

R

K

A

V

L

L

-

E

G

N

Y

T

K

E

T

I

G

G

N

D

S

S

L

I

V

F

E

D

E

K

E

A

L

V

I

G

D

P

E

E

A

I

K

A

R

K

V

L

M

M

D

E

E

K

A

A

G

K

R

A

K

K

G

G

V

V

K

E

L

V

Y

V

L

L

P

P

V

V

D

D

F

F

V

I

C

I

S

A

T

D

S

A

-
x
F

P

S

E

A

D

S

E

A

S

N

G

T

Y

K

V

T

I

V

K

T

P

D

Y

K

Q

E

E

G

I

I

P

P

K

A

G

G

M

W

M

Q

G

G

L
|
L

D

D

I

N

G

G

P

P

A

E

S

S

I

R

E

K

L

L

F

F

R

A

E

A

V

T

L

V

S

A

D

K

A

A

K

T

V

V

V

I

V

L

W

W

N
|
N

G
|
G

P
|
P

M

P

G
|
G

V
|
V

F

F

E

E

V

F

K

E

A

K

F

F

A

A

H

A

G

G

T

T

N

K

R

A

I

L

A

L

-

D

Q

E

I

V

I

V

G

K

S

S

L

S

N

A

A

A

-

G

-

N

L

T

K

V

V

I

V

I

G

G

G

G

G
|
G

D
|
D

T

T

A

A

D

T

A

V

V

A

E

K

R

K

A

Y

G

G

E

V

S

T

D

D

N

K

M

I

S

S

Y

H

I

V

S

S

T

T

G

G

G

G

G
|
G

A

A

S

S

L

L

K

E

L

L

L

L

E

E

G

G

K

K

T

E

L

L

P

P

A

G

V

V

E

A

V

F

L

L

E

S

K

E

K

K

Q

K

active site: R38 (= R35), K213 (= K197), G371 (= G350), G394 (= G373)
binding adenosine-5'-triphosphate: G211 (= G195), A212 (= A196), K213 (= K197), F289 (vs. gap), L311 (= L293), N334 (= N316), G335 (= G317), P336 (= P318), G338 (= G320), V339 (= V321), D372 (= D351)

P09041 Phosphoglycerate kinase 2; Phosphoglycerate kinase, testis specific; EC 2.7.2.3 from Mus musculus (Mouse) (see paper)
45% identity, 98% coverage: 3:390/394 of query aligns to 8:414/417 of P09041

query
sites
P09041

V

T

I

L

N

D

E

K

I

V

D

D

I

L

T

K

G

G

K

K

R

R

V

V

F

I

I

M

R

R

C

V

D

D

F

F

N

N

V

V

P

P

M

M

D

-

E

K

N

N

G

N

N

Q

I

I

T

T

D

N

D

N

N

Q

R

R

I

I

R

K

A

A

A

A

L

I

P

P

T

S

I

I

Q

K

Y

H

A

C

I

L

D

D

N

N

K

G

A

A

K

K

-

S

V

V

I

V

L

L

A

M

S

S

H

H

L

L

G

G

R

R

P

P

K

D

G

G

K

I

R

P

N

M

P

P

-

D

K

K

Y

Y

S

S

L

L

K

E

P

P

V

V

A

A

K

D

R

E

L

L

A

K

T

S

I

L

L

L

G

N

K

K

P

D

V

V

L

I

F

F

I

L

E

K

D

D

F

C

E

V

N

G

P

P

E

E

D

V

K

E

A

Q

K

A

L

C

E

A

A

N

L

P

K

D

D

N

G

G

D

S

V

I

A

I

L

L

L

L

E

E

N

N

L

L

R

R

F

F

W

H

E

V

G

E

E

E

E

E

K

G

N

K

S

G

K

K

E

D

-

S

-

S

-

G

-

K

-

K

-

I

-

S

-

A

-

D

-

P

-

A

-

K

-

V

-

E

-

A

F

F

A

Q

L

A

K

S

L

L

K

S

E

K

L

L

F

G

D

D

V

V

Y

Y

V

V

N

N

D

D

A

A

F

F

G

G

V

T

C

A

H

H

R

R

K

A

H

H

A

S

S

S

V

T

Y

V

A

G

L

V

A

N

E

L

I

P

S

Q

E

K

I

-

A

-

A

A

I

S

G

G

F

F

L

L

L

M

K

K

K

K

E

E

L

L

E

D

Y

Y

F

F

D

S

K

K

I

A

F

L

T

E

I

K

E

P

E

E

K

R

P

P

F

F

V

L

A

A

V

I

I

L

G

G

G

G

A

A

K

K

I

V

S

K

G

D

K
|
K

L

I

D

Q

C

L

L

I

L

K

N

N

L

M

L

L

N

D

R

K

A

V

D

N

K

F

I

M

I

I

I

I

G

G

G

G

A

G

Q

M

A

A

F

Y

T

T

F

F

L

L

R

K

A

E

L

L

-

K

G

N

Y

M

K

Q

T

I

G

G

N

A

S

S

L

L

V

F

E

D

E

E

E

E

L

G

I

A

D

T

E

I

A

V

K

K

R

E

V

I

M

M

D

E

E

K

A

A

G

E

R

K

K

N

G

G

V

V

K

K

L

I

Y

V

L

F

P

P

V

V

D

D

F

F

V

V

C

-

S

T

T

G

S

D

P

K

E

F

D

D

E

E

S

N

G

A

Y

K

V

V

I

G

K

Q

P

A

Y

T

Q

I

E

E

-

S

-

G

I

I

P

P

K

S

G

G

M

W

M

M

G
|
G

L

L

D

D

I

C

G

G

P

P

A

E

S

S

I

I

E

K

L

I

F

N

R

A

E

Q

V

I

L

V

S

A

D

Q

A

A

K

K

V

L

V

I

V

V

W

W

N

N

G

G

P

P

M

I

G

G

V

V

F

F

E
|
E

V

W

K

D

A

A

F

F

A

A

H

K

G

G

T

T

N

K

R

A

I

L

A

M

Q

D

I

E

I

V

-

V

-

K

-

A

G

T

S

S

L

N

N

G

A

C

L

V

K

T

V

I

V

I

G

G

G
|
G

G
|
G

D
|
D

T
|
T

A

A

D

T

A

C

V

C

E

A

R

K

A

W

G

G

E

T

S

E

D

D

N

K

M

V

S

S

Y

H

I

V

S

S

T

T

G

G

G

G

G

G

A

A

S

S

L

L

K

E

L

L

L

L

E

E

G

G

K

K

T

I

L

L

P

P

A

G

V

V

E

E

V

A

L

L

K220 (= K201) binding ATP
G313 (= G292) binding ATP
E344 (= E323) binding ATP
GGDT 373:376 (= GGDT 349:352) binding ATP

2paaA Crystal structure of phosphoglycerate kinase-2 bound to atp and 3pg (see paper)
45% identity, 98% coverage: 3:390/394 of query aligns to 4:410/413 of 2paaA

query
sites
2paaA

V

T

I

L

N

D

E

K

I

V

D

D

I

L

T

K

G

G

K

K

R

R

V

V

F

I

I

M

R

R

C

V

D

D

F

F

N

N

V

V

P

P

M

M

D

-

E

K

N

N

G

N

N

Q

I

I

T

T

D

N

D

N

N

Q

R
|
R

I

I

R

K

A

A

A

A

L

I

P

P

T

S

I

I

Q

K

Y

H

A

C

I

L

D

D

N

N

K

G

A

A

K

K

-

S

V

V

I

V

L

L

A

M

S

S

H

H

L

L

G

G

R

R

P

P

K

D

G

G

K

I

R

P

N

M

P

P

-

D

K

K

Y

Y

S

S

L

L

K

E

P

P

V

V

A

A

K

D

R

E

L

L

A

K

T

S

I

L

L

L

G

N

K

K

P

D

V

V

L

I

F

F

I

L

E

K

D

D

F

C

E

V

N

G

P

P

E

E

D

V

K

E

A

Q

K

A

L

C

E

A

A

N

L

P

K

D

D

N

G

G

D

S

V

I

A

I

L

L

L

L

E

E

N

N

L

L

R

R

F

F

W

H

E

V

G

E

E

E

E

E

K

G

N

K

S

G

K

K

E

D

-

S

-

S

-

G

-

K

-