SitesBLAST
Comparing WP_022671214.1 NCBI__GCF_000420385.1:WP_022671214.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
Q72IB8 Proline dehydrogenase; PRODH; Proline oxidase; TtPRODH; EC 1.5.5.2 from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see 2 papers)
37% identity, 89% coverage: 23:285/296 of query aligns to 31:295/307 of Q72IB8
2g37B Structure of thermus thermophilus l-proline dehydrogenase (see paper)
37% identity, 88% coverage: 25:284/296 of query aligns to 39:300/300 of 2g37B
- binding flavin-adenine dinucleotide: D139 (= D125), M140 (= M126), V167 (= V151), R190 (= R174), V192 (≠ C176), K193 (= K177), G194 (= G178), A195 (≠ I179), A231 (= A215), T232 (= T216), H233 (= H217), D234 (= D218), L260 (= L244), V263 (= V247), Y281 (= Y265)
Q9RW55 Proline dehydrogenase; PRODH; DrPRODH; Proline oxidase; EC 1.5.5.2 from Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / CCUG 27074 / LMG 4051 / NBRC 15346 / NCIMB 9279 / VKM B-1422 / R1) (see paper)
35% identity, 100% coverage: 2:296/296 of query aligns to 4:309/310 of Q9RW55
- G63 (= G56) mutation to A: 140-fold decrease in catalytic efficiency for proline.
- E64 (= E57) mutation to A: 27-fold decrease in catalytic efficiency for proline.
- K98 (= K91) binding substrate
- M136 (= M126) binding FAD
- Q166 (= Q153) binding FAD
- RMVKGA 187:192 (≠ RLCKGI 174:179) binding FAD
- TH 229:230 (= TH 216:217) binding FAD
- RR 291:292 (= RR 278:279) binding substrate
- RIAE 292:295 (≠ RLKE 279:282) binding FAD
4h6qA Structure of oxidized deinococcus radiodurans proline dehydrogenase complexed with l-tetrahydrofuroic acid (see paper)
35% identity, 92% coverage: 12:284/296 of query aligns to 3:281/281 of 4h6qA
- binding flavin-adenine dinucleotide: D119 (= D125), M120 (= M126), Q150 (= Q153), R171 (= R174), V173 (≠ C176), K174 (= K177), G175 (= G178), A176 (≠ I179), A212 (= A215), T213 (= T216), H214 (= H217), M240 (= M243), L241 (= L244), I244 (≠ V247), R276 (= R279), E279 (= E282), P281 (= P284)
- binding tetrahydrofuran-2-carboxylic acid: K82 (= K91), Y262 (= Y265), R275 (= R278), R276 (= R279)
4h6rA Structure of reduced deinococcus radiodurans proline dehydrogenase (see paper)
37% identity, 89% coverage: 23:284/296 of query aligns to 5:272/272 of 4h6rA
- binding dihydroflavine-adenine dinucleotide: D110 (= D125), M111 (= M126), V139 (= V151), Q141 (= Q153), R162 (= R174), V164 (≠ C176), K165 (= K177), G166 (= G178), A167 (≠ I179), A203 (= A215), T204 (= T216), H205 (= H217), Q230 (= Q242), L232 (= L244), I235 (≠ V247), Y253 (= Y265), R267 (= R279), E270 (= E282), P272 (= P284)
5m42A Structure of thermus thermophilus l-proline dehydrogenase lacking alpha helices a, b and c (see paper)
36% identity, 80% coverage: 33:269/296 of query aligns to 4:242/242 of 5m42A
- binding flavin mononucleotide: D96 (= D125), M97 (= M126), V124 (= V151), Q126 (= Q153), R147 (= R174), V149 (≠ C176), K150 (= K177), G151 (= G178), A152 (≠ I179), A188 (= A215), T189 (= T216), H190 (= H217), L217 (= L244)
4nmfB Crystal structure of proline utilization a (puta) from geobacter sulfurreducens pca inactivated by n-propargylglycine and complexed with menadione bisulfite (see paper)
27% identity, 92% coverage: 11:283/296 of query aligns to 84:407/979 of 4nmfB
- binding (2R)-2-methyl-1,4-dioxo-1,2,3,4-tetrahydronaphthalene-2-sulfonic acid: K184 (= K91), Y290 (= Y180), Y387 (= Y265), Y399 (= Y275), R402 (= R278), R403 (= R279)
- binding (2S)-2-methyl-1,4-dioxo-1,2,3,4-tetrahydronaphthalene-2-sulfonic acid: K184 (= K91), L366 (= L244), Y399 (= Y275), R402 (= R278)
- binding N-propargylglycine-modified flavin adenine dinucleotide: K184 (= K91), D225 (= D125), M226 (= M126), V255 (= V151), Q257 (= Q153), R284 (= R174), V286 (≠ C176), K287 (= K177), G288 (= G178), A289 (≠ I179), W291 (≠ D181), W308 (≠ F198), T309 (≠ I199), I310 (≠ Y200), K311 (≠ C201), S314 (≠ T204), A337 (= A215), S338 (≠ T216), H339 (= H217), N340 (≠ D218), Q364 (= Q242), L366 (= L244), Y387 (= Y265), E406 (= E282)
Sites not aligning to the query:
4nmdA Crystal structure of proline utilization a (puta) from geobacter sulfurreducens pca reduced with dithionite (see paper)
27% identity, 92% coverage: 11:283/296 of query aligns to 85:408/979 of 4nmdA
- binding dihydroflavine-adenine dinucleotide: D226 (= D125), M227 (= M126), V256 (= V151), Q258 (= Q153), R285 (= R174), V287 (≠ C176), K288 (= K177), G289 (= G178), A290 (≠ I179), W292 (≠ D181), W309 (≠ F198), T310 (≠ I199), I311 (≠ Y200), K312 (≠ C201), S315 (≠ T204), A338 (= A215), S339 (≠ T216), H340 (= H217), N341 (≠ D218), Q365 (= Q242), V366 (≠ M243), L367 (= L244), Y388 (= Y265)
Sites not aligning to the query:
4nmfA Crystal structure of proline utilization a (puta) from geobacter sulfurreducens pca inactivated by n-propargylglycine and complexed with menadione bisulfite (see paper)
27% identity, 92% coverage: 11:283/296 of query aligns to 81:404/973 of 4nmfA
- binding (2R)-2-methyl-1,4-dioxo-1,2,3,4-tetrahydronaphthalene-2-sulfonic acid: K181 (= K91), Y287 (= Y180), Y384 (= Y265), Y396 (= Y275), R399 (= R278), R400 (= R279)
- binding N-propargylglycine-modified flavin adenine dinucleotide: K181 (= K91), D222 (= D125), M223 (= M126), V252 (= V151), Q254 (= Q153), R281 (= R174), V283 (≠ C176), K284 (= K177), G285 (= G178), A286 (≠ I179), W288 (≠ D181), W305 (≠ F198), T306 (≠ I199), I307 (≠ Y200), K308 (≠ C201), S311 (≠ T204), A334 (= A215), S335 (≠ T216), H336 (= H217), N337 (≠ D218), Q361 (= Q242), V362 (≠ M243), L363 (= L244), Y384 (= Y265), E403 (= E282)
Sites not aligning to the query:
7na0A Structure of geobacter sulfurreducens proline utilization a (puta) variant a206w (see paper)
26% identity, 93% coverage: 9:283/296 of query aligns to 83:408/981 of 7na0A
- binding flavin-adenine dinucleotide: D226 (= D125), M227 (= M126), V256 (= V151), Q258 (= Q153), R285 (= R174), V287 (≠ C176), K288 (= K177), G289 (= G178), A290 (≠ I179), Y291 (= Y180), W292 (≠ D181), W309 (≠ F198), T310 (≠ I199), I311 (≠ Y200), K312 (≠ C201), S315 (≠ T204), A338 (= A215), S339 (≠ T216), H340 (= H217), N341 (≠ D218), L367 (= L244), Y388 (= Y265), E407 (= E282)
Sites not aligning to the query:
5ur2B Crystal structure of proline utilization a (puta) from bdellovibrio bacteriovorus inactivated by n-propargylglycine (see paper)
26% identity, 89% coverage: 20:283/296 of query aligns to 86:399/959 of 5ur2B
- binding N-propargylglycine-modified flavin adenine dinucleotide: K174 (= K91), D215 (= D125), M216 (= M126), Q249 (= Q153), V278 (≠ C176), K279 (= K177), G280 (= G178), A281 (≠ I179), W283 (≠ D181), Y300 (= Y188), T301 (≠ K189), N302 (≠ E190), K303 (= K191), S306 (≠ V194), A329 (= A215), S330 (≠ T216), H331 (= H217), N332 (≠ D218), Q356 (= Q242), M357 (= M243), L358 (= L244), Y379 (= Y265), E398 (= E282)
Sites not aligning to the query:
4nmaA Crystal structure of proline utilization a (puta) from geobacter sulfurreducens pca in complex with l-tetrahydro-2-furoic acid (see paper)
26% identity, 92% coverage: 12:283/296 of query aligns to 86:408/977 of 4nmaA
- binding flavin-adenine dinucleotide: D226 (= D125), M227 (= M126), Q258 (= Q153), R285 (= R174), V287 (≠ C176), K288 (= K177), G289 (= G178), A290 (≠ I179), Y291 (= Y180), W292 (≠ D181), W309 (≠ F198), T310 (≠ I199), I311 (≠ Y200), K312 (≠ C201), S315 (≠ T204), A338 (= A215), S339 (≠ T216), H340 (= H217), N341 (≠ D218), Q365 (= Q242), L367 (= L244), E407 (= E282)
- binding tetrahydrofuran-2-carboxylic acid: K185 (= K91), Y388 (= Y265), Y400 (= Y275), R403 (= R278), R404 (= R279)
Sites not aligning to the query:
4nmcA Crystal structure of oxidized proline utilization a (puta) from geobacter sulfurreducens pca complexed with zwittergent 3-12 (see paper)
26% identity, 96% coverage: 11:293/296 of query aligns to 74:410/941 of 4nmcA
- binding flavin-adenine dinucleotide: D215 (= D125), M216 (= M126), V245 (= V151), Q247 (= Q153), R274 (= R174), V276 (≠ C176), K277 (= K177), G278 (= G178), A279 (≠ I179), W281 (≠ D181), W298 (≠ F198), T299 (≠ I199), I300 (≠ Y200), K301 (≠ C201), S304 (≠ T204), A327 (= A215), S328 (≠ T216), H329 (= H217), N330 (≠ D218), L356 (= L244), Y377 (= Y265)
Sites not aligning to the query:
4nmeA Crystal structure of proline utilization a (puta) from geobacter sulfurreducens pca inactivated by n-propargylglycine (see paper)
26% identity, 93% coverage: 9:283/296 of query aligns to 81:403/972 of 4nmeA