SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_022948641.1 NCBI__GCF_000421465.1:WP_022948641.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P16703 Cysteine synthase B; CSase B; O-acetylserine (thiol)-lyase B; OAS-TL B; O-acetylserine sulfhydrylase B; EC 2.5.1.47 from Escherichia coli (strain K12) (see paper)
68% identity, 96% coverage: 12:301/303 of query aligns to 3:292/303 of P16703

query
sites
P16703

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N71 (= N80) binding pyridoxal 5'-phosphate
S255 (= S264) binding pyridoxal 5'-phosphate

2bhtA Crystal structure of o-acetylserine sulfhydrylase b (see paper)
67% identity, 96% coverage: 10:301/303 of query aligns to 1:292/294 of 2bhtA

query
sites
2bhtA

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active site: K41 (= K50), S69 (= S78), Q199 (= Q208), G203 (= G212), S255 (= S264), C280 (= C289)
binding pyridoxal-5'-phosphate: K41 (= K50), N71 (= N80), M173 (= M182), G174 (= G183), T175 (= T184), T176 (= T185), T178 (= T187), G208 (= G217), S255 (= S264), C280 (= C289)

5xoqA Crystal structure of o-acetylserine sulfhydrylase with bound transcription factor peptide inhibitor from planctomyces limnophilus
46% identity, 94% coverage: 17:301/303 of query aligns to 12:306/310 of 5xoqA

query
sites
5xoqA

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binding : T72 (= T77), S73 (= S78), G74 (= G79), T76 (= T81), M123 (= M128), Q144 (= Q149), R218 (vs. gap), H219 (vs. gap), Q222 (≠ R219), G223 (≠ R220), A226 (≠ P223)

P47999 Cysteine synthase, chloroplastic/chromoplastic; At.OAS.7-4; Beta-substituted Ala synthase 2;1; ARAth-Bsas2;1; CSase B; AtCS-B; CS-B; O-acetylserine (thiol)-lyase; O-acetylserine sulfhydrylase; OAS-TL B; cpACS1; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
44% identity, 94% coverage: 17:301/303 of query aligns to 83:378/392 of P47999

query
sites
P47999

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Sites not aligning to the query:

61 modified: N-acetylalanine

7n2tA O-acetylserine sulfhydrylase from citrullus vulgaris in the internal aldimine state, with citrate bound (see paper)
45% identity, 94% coverage: 17:301/303 of query aligns to 11:306/309 of 7n2tA

query
sites
7n2tA

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E

A

P

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

G

L

L

A

A

M

F

V

I

A

A

T

A

I

A

K

K

G

G

Y

Y

R

R

M

L

T

I

L

I

I

C

M

M

P

P

E

A

N

S

M

M

S

S

V

L

E

E

R

R

A

T

V

I

M

L

K

R

A

A

F

F

G

G

A

A

E

E

I

L

L

V

L

L

V

T

S

D

P

P

E

A

G

R

G

G

M

M

E

K

E

G

A

A

R

V

D

Q

L

K

A

A

D

E

A

E

M

I

G

K

E

A

R

K

G

T

-

P

E

N

G

S

K

Y

V

I

L

L

N

Q

Q

Q

F

F

A

E

N

N

P

P

D

A

N

N

P

P

Q

K

A

I

H

H

Y

Y

E

E

G

T

T

T

G

G

P

P

E

E

I

I

W

W

R

R

D

G

T

S

K

G

G

G

T

K

I

I

T

D

H

A

F

L

V

V

S

S

S

G

M

I

G

G

T

T

T

G

G

G

T

T

I

V

M

T

G

G

V

A

S

G

K

K

F

Y

F

L

K

K

E

E

K

Q

K

N

P

P

A

N

I

I

Q

K

I

L

V

Y

G

G

V

V

Q

E

P

P

V

V

E

E

G

S

A

A

K

I

I

L

P

S

G

G

I

G

R

K

R

P

W

G

P

P

P

H

E

K

-

I

-

Q

-

G

-

I

-

G

-

A

-

G

Y

F

L

I

P

P

K

G

I

V

Y

L

E

D

P

V

S

N

R

L

V

L

D

D

R

E

Q

V

L

I

D

Q

V

V

T

S

Q

S

Q

E

E

E

A

S

E

I

E

E

T

T

T

A

R

K

R

L

L

L

A

A

A

L

E

K

E

E

G

G

I

L

F

L

C

V

G

G

I

I

S

S

G

G

G

A

A

A

V

A

A

A

L

I

R
|
R

L

I

S

A

A

K

E
x
R

V

P

E

E

N

N

A

A

-

G

-

K

V

L

I

I

V

V

T

A

I

V

V

F

C

P

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

V

V

L

F

F

binding phosphate ion: R277 (= R274), R281 (≠ E278)

4aecA Crystal structure of the arabidopsis thaliana o-acetyl-serine-(thiol)- lyasE C (see paper)
43% identity, 96% coverage: 12:301/303 of query aligns to 16:316/323 of 4aecA

query
sites
4aecA

T

N

I

V

E

S

A

Q

C

L

V

I

G

G

N

K

T

T

P

P

L

M

V

V

H

Y

L

L

Q

N

R

S

L

I

P

A

G

K

D

G

T

C

R

V

N

A

V

N

I

I

L

A

A

A

K

K

L

L

E

E

G

I

N

M

N

E

P

P

A

C

G

C

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

Y

S

S

M

M

I

V

Q

T

H

D

A

A

Q

E

E

Q

R

K

G

G

E

F

I

I

K

S

P

P

G

G

D

K

R

S

-

V

L

L

I

V

E

E

A

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

G

L

L

A

A

M

F

V

I

A

A

T

A

I

S

K

R

G

G

Y

Y

R

R

M

L

T

I

L

L

I

T

M

M

P

P

E

A

N

S

M

M

S

S

V

M

E

E

R

R

A

V

V

L

M

L

K

K

A

A

F

F

G

G

A

A

E

E

I

L

L

V

L

L

V

T

S

D

P

P

E

A

G

K

G

G

M

M

E

T

E

G

A

A

R

V

D

Q

L

K

A

A

D

E

A

E

M

I

G

L

E

K

R

N

G

T

-

P

E

D

G

A

K

Y

V

M

L

L

N

Q

Q

Q

F

F

A

D

N

N

P

P

D

A

N

N

P

P

Q

K

A

I

H

H

Y

Y

E

E

G

T

T

T

G

G

P

P

E

E

I

I

W

W

R

D

D

D

T

T

K

K

G

G

T

K

I

V

T

D

H

I

F

F

V

V

S

A

S

G

M
x
I

G
|
G

T
|
T

T
x
G

G
|
G

T
|
T

I

I

M

T

G

G

V

V

S

G

K

R

F

F

F

I

K

K

E

E

K

K

K

N

P

P

A

K

I

T

Q

Q

I

V

V

I

G

G

V

V

Q

E

P

P

V

T

E

E

G

S

A

D

K

I

I

L

P

S

G

G

I

G

R

K

R

P

W

G

P

P

P

H

E

K

-

I

-

Q

-
x
G

-

I

-

G

-

A

-

G

Y

F

L

I

P

P

K

K

I

N

Y

L

E

D

P

Q

S

K

R

I

V

M

D

D

R

E

Q

V

L

I

D

A

V

I

T

S

Q

S

Q

E

E

E

A

A

E

I

E

E

T

T

T

A

R

K

R

Q

L

L

A

A

A

L

E

K

E

E

G

G

I

L

F

M

C

V

G

G

I

I

S
|
S

S

S

G

G

G

A

A

A

V

A

A

A

L

I

R

K

L

V

S

A

A

K

E

R

V

P

E

E

N

N

A

A

-

G

-

K

V

L

I

I

V

A

T

V

I

V

V

F

C
x
P

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

P

V

L

F

F

active site: K54 (= K50), S277 (= S264)
binding pyridoxal-5'-phosphate: K54 (= K50), N85 (= N80), I188 (≠ M182), G189 (= G183), T190 (= T184), G191 (≠ T185), G192 (= G186), T193 (= T187), G233 (vs. gap), S277 (= S264), P304 (≠ C289)

P47998 Cysteine synthase 1; At.OAS.5-8; Beta-substituted Ala synthase 1;1; ARAth-Bsas1;1; CSase A; AtCS-A; Cys-3A; O-acetylserine (thiol)-lyase 1; OAS-TL A; O-acetylserine sulfhydrylase; Protein ONSET OF LEAF DEATH 3; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
43% identity, 95% coverage: 16:303/303 of query aligns to 12:310/322 of P47998

query
sites
P47998

C

L

V

I

G

G

N

N

T

T

P

P

L

L

V

V

H

Y

L

L

Q

N

R

N

L

V

P

A

G

E

D

G

T

C

R

V

N

G

V

R

I

V

L

A

A

A

K

K

L

L

E

E

G

M

N

M

N

E

P

P

A

C

G

S

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

F

S

S

M

M

I

I

Q

S

H

D

A

A

Q

E

E

K

R

K

G

G

E

L

I

I

K

K

P

P

G

G

D

E

R

S

-

V

L

L

I

I

E

E

A

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

M

F

V

T

A

A

T

A

I

A

K

K

G

G

Y

Y

R

K

M

L

T

I

L

I

I

T

M

M

P

P

E

A

N

S

M

M

S

S

V

T

E

E

R

R

A

I

V

I

M

L

K

L

A

A

F

F

G

G

A

V

E

E

I

L

L

V

L

L

V

T

S

D

P

P

E

A

G

K

G

G

M

M

E

K

E

G

A

A

R

I

D

A

L

K

A

A

D

E

-

E

A

I

M

L

G

A

E

K

R

T

G

P

E

N

G

G

K

Y

V

M

L

L

N

Q

Q
|
Q

F

F

A

E

N

N

P

P

D

A

N

N

P

P

Q

K

A

I

H
|
H

Y

Y

E

E

G

T

T

T

G
|
G

P

P

E

E

I

I

W

W

R

K

D

G

T

T

K

G

G

G

T

K

I

I

T

D

H

G

F

F

V

V

S

S

S

G

M

I

G
|
G

T
|
T

T
x
G

G
|
G

T
|
T

I

I

M

T

G

G

V

A

S

G

K

K

F

Y

F

L

K

K

E

E

K

Q

K

N

P

A

A

N

I

V

Q

K

I

L

V

Y

G

G

V

V

Q

E

P

P

V

V

E

E

G

S

A

A

K

I

I

L

P

S

G

G

I

G

R
x
K

R

P

W

G

P

P

P
x
H

E
x
K

-

I

-

Q

-

G

-

I

-

G

-

A

-

G

Y

F

L

I

P

P

K

S

I

V

Y

L

E

N

P

V

S

D

R

L

V

I

D

D

R

E

Q

V

L

V

D

Q

V

V

T

S

Q

S

Q

D

E

E

A

S

E

I

E

D

T

M

T

A

R

R

R

Q

L

L

A

A

A

L

E

K

E

E

G

G

I

L

F

L

C

V

G

G

I

I

S
|
S

S

S

G

G

G

A

A

A

V

A

A

A

L

I

R

K

L

L

S

A

A

Q

E

R

V

P

E

E

N

N

A

A

-

G

-

K

V

L

I

F

V

V

T

A

I

I

V

F

C

P

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

V

V

L

F

F

P

D

A

A

K46 (= K50) modified: N6-(pyridoxal phosphate)lysine; mutation to A: No cysteine synthase activity.
T74 (= T77) mutation to A: Strong reduction of cysteine synthase activity.; mutation to S: Reduction of cysteine synthase activity.
S75 (= S78) mutation S->A,N,T: Strong reduction of cysteine synthase activity.
N77 (= N80) binding pyridoxal 5'-phosphate; mutation to A: Reduction of cysteine synthase activity.; mutation to D: Strong reduction of cysteine synthase activity.
T78 (= T81) mutation T->A,S: Reduction of cysteine synthase activity.
Q147 (= Q149) mutation Q->A,E: Strong reduction of cysteine synthase activity.
H157 (= H159) mutation H->Q,N: Slight reduction of cysteine synthase activity.
G162 (= G164) mutation to E: In old3-1; displays a early leaf death phenotype. Abolishes cysteine synthase activity.
GTGGT 181:185 (≠ GTTGT 183:187) binding pyridoxal 5'-phosphate
T182 (= T184) mutation T->A,S: Slight reduction of cysteine synthase activity.
T185 (= T187) mutation T->A,S: Strong reduction of cysteine synthase activity.
K217 (≠ R219) mutation to A: Impaired interaction with SAT1.
H221 (≠ P223) mutation to A: Impaired interaction with SAT1.
K222 (≠ E224) mutation to A: Impaired interaction with SAT1.
S269 (= S264) binding pyridoxal 5'-phosphate; mutation to A: Strong reduction of cysteine synthase activity.; mutation to T: Reduction of cysteine synthase activity.

2isqA Crystal structure of o-acetylserine sulfhydrylase from arabidopsis thaliana in complex with c-terminal peptide from arabidopsis serine acetyltransferase (see paper)
43% identity, 95% coverage: 16:303/303 of query aligns to 10:308/320 of 2isqA

query
sites
2isqA

C

L

V

I

G

G

N

N

T

T

P

P

L

L

V

V

H

Y

L

L

Q

N

R

N

L

V

P

A

G

E

D

G

T

C

R

V

N

G

V

R

I

V

L

A

A

A

K

K

L

L

E

E

G

M

N

M

N

E

P

P

A

C

G

S

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

F

S

S

M

M

I

I

Q

S

H

D

A

A

Q

E

E

K

R

K

G

G

E

L

I

I

K

K

P

P

G

G

D

E

R

S

-

V

L

L

I

I

E

E

A

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

M

F

V

T

A

A

T

A

I

A

K

K

G

G

Y

Y

R

K

M

L

T

I

L

I

I

T

M

M

P

P

E

A

N

S

M

M

S

S

V

T

E

E

R

R

A

I

V

I

M

L

K

L

A

A

F

F

G

G

A

V

E

E

I

L

L

V

L

L

V

T

S

D

P

P

E

A

G

K

G
|
G

M
|
M

E
x
K

E

G

A

A

R

I

D

A

L

K

A

A

D

E

-

E

A

I

M

L

G

A

E

K

R

T

G

P

E

N

G

G

K

Y

V

M

L

L

N

Q

Q

Q

F

F

A

E

N

N

P

P

D

A

N

N

P

P

Q

K

A

I

H

H

Y

Y

E

E

G

T

T

T

G

G

P

P

E

E

I

I

W

W

R

K

D

G

T

T

K

G

G

G

T

K

I

I

T

D

H

G

F

F

V

V

S

S

S
x
G

M

I

G
|
G

T
|
T

T
x
G

G

G

T
|
T

I

I

M

T

G

G

V

A

S

G

K

K

F

Y

F

L

K

K

E

E

K

Q

K

N

P

A

A

N

I

V

Q

K

I

L

V

Y

G

G

V

V

Q

E

P

P

V

V

E

E

G

S

A

A

K

I

I

L

P

S

G

G

I

G

R

K

R

P

W
x
G

P
|
P

P
x
H

E

K

-

I

-
x
Q

-
x
G

-

I

-

G

-

A

-

G

Y

F

L

I

P

P

K

S

I

V

Y

L

E

N

P

V

S

D

R

L

V

I

D

D

R

E

Q

V

L

V

D

Q

V

V

T

S

Q

S

Q

D

E

E

A

S

E

I

E

D

T

M

T

A

R

R

R

Q

L

L

A

A

A

L

E

K

E

E

G

G

I

L

F

L

C

V

G

G

I

I

S
|
S

S

S

G

G

G

A

A

A

V

A

A

A

L

I

R

K

L

L

S

A

A

Q

E

R

V

P

E

E

N

N

A

A

-

G

-

K

V

L

I

F

V

V

T

A

I

I

V

F

C
x
P

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

V

V

L

F

F

P

D

A

A

active site: K44 (= K50), S267 (= S264)
binding pyridoxal-5'-phosphate: K44 (= K50), N75 (= N80), G177 (≠ S181), G179 (= G183), T180 (= T184), G181 (≠ T185), T183 (= T187), G223 (vs. gap), S267 (= S264), P294 (≠ C289)
binding : T72 (= T77), S73 (= S78), G74 (= G79), T76 (= T81), G122 (= G127), M123 (= M128), K124 (≠ E129), G217 (≠ W221), P218 (= P222), H219 (≠ P223), Q222 (vs. gap), G223 (vs. gap)

8b9yC Cysteine synthase from trypanosoma cruzi with plp and oas (see paper)
42% identity, 96% coverage: 11:301/303 of query aligns to 12:312/330 of 8b9yC

query
sites
8b9yC

P

P

T

S

I

M

E

D

A

A

C

L

V

I

G

G

N

E

T

T

P

P

L

A

V

V

H

Y

L

L

Q

K

R

R

L

M

P

-

G

N

D

D

T

T

R

A

N

A

V

T

I

I

L

V

A

L

K

K

L

L

E

E

G

C

N

E

N

N

P

P

A

M

G

A

S

S

V

V

K
|
K

D

D

R

R

P

L

A

A

L

Y

S

A

M

I

I

Y

Q

D

H

K

A

A

Q

E

E

K

R

E

G

G

E

K

I

I

K

I

P

P

G

G

D

K

R

S

L

V

I

I

-

V

E

E

A

A

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

M

H

V

I

A

G

T

T

I

I

K

R

G

G

Y

Y

R

K

M

V

T

I

L

I

I

V

M

M

P

P

E

E

N

S

M

M

S

S

V

I

E

E

R

R

A

C

V

L

M

M

K

R

A

I

F

F

G

G

A

A

E

E

I

V

L

I

L

L

V

T

S

P

P

A

E

A

G

L

G

G

M

M

E

K

E

G

A

A

R

L

D

E

L

A

A

V

D

N

A

R

M

I

G

V

E

S

R

N

G

N

E

P

G

D

K

A

V

V

-

S

L

A

N

N

Q

Q

F

F

A

A

N

T

P

K

D

Y

N

N

P

A

Q

Q

A

I

H

H

Y

E

E

E

G

T

T

T

G

G

P

P

E

E

I

I

W

W

R

R

D

Q

T

T

K

K

G

G

T

H

I

V

T

D

H

C

F

F

V

V

S

A

S

G

M

V

G
|
G

T
|
T

T
x
G

G

G

T
|
T

I

I

M

T

G

G

V

V

S

A

K

R

F

Y

F

L

K

K

E

S

K

V

K

G

P

C

A

G

I

A

Q

T

I

I

V

F

G

A

V

V

Q

E

P

P

V

A

E

E

G

S

A

P

K

V

I

L

P

S

G

G

I

G

R

K

R

P

W

G

P

P

P

H

E

R

-

I

-

Q

-
x
G

-

I

-

G

-

A

-

G

Y

F

L

V

P

P

K

E

I

V

Y

F

E

E

P

A

S

A

R

L

V

V

D

D

R

E

Q

V

L

I

D

Q

V

V

T

S

Q

G

Q

D

E

E

A

A

E

I

E

D

T

T

T

A

R

Q

R

K

L

L

A

P

A

R

E

T

E

D

G

G

I

I

F

F

C

C

G

G

I

F

S
|
S

S

G

G

G

G

A

A

N

V

V

A

Y

A

A

L

L

R

Q

L

I

S

A

A

K

-

R

-

P

E

E

V

M

E

A

N

G

A

K

V

T

I

I

V

V

T

T

I

V

V

I

C
x
P

D
x
S

R

Y

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

A

V

L

F

Y

binding pyridoxal-5'-phosphate: K50 (= K50), N81 (= N80), G185 (= G183), T186 (= T184), G187 (≠ T185), T189 (= T187), G229 (vs. gap), S273 (= S264), P300 (≠ C289), S301 (≠ D290)

8b9yA Cysteine synthase from trypanosoma cruzi with plp and oas (see paper)
42% identity, 96% coverage: 11:301/303 of query aligns to 10:295/313 of 8b9yA

query
sites
8b9yA

P

P

T

S

I

M

E

D

A

A

C

L

V

I

G

G

N

E

T

T

P

P

L

A

V

V

H

Y

L

L

Q

K

R

R

L

M

P

-

G

N

D

D

T

T

R

A

N

A

V

T

I

I

L

V

A

L

K

K

L

L

E

E

G
x
C

N
x
E

N
|
N

P
|
P

A

M

G
x
A

S

S

V
|
V

K
|
K

D

D

R

R

P

L

A

A

L

Y

S

A

M

I

I

Y

Q

D

H

K

A

A

Q

E

E

K

R

E

G

G

E

K

I

I

K

I

P

P

G

G

D

K

R

S

L

V

I

I

-

V

E

E

A

A

T
|
T

S

S

G
|
G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

M

H

V

I

A

G

T

T

I

I

K

R

G

G

Y

Y

R

K

M

V

T

I

L

I

I

V

M

M

P

P

E

E

N

S

M

M

S

S

V

I

E

E

R

R

A

C

V

L

M

M

K

R

A

I

F

F

G

G

A

A

E

E

I

V

L

I

L

L

V

T

S

P

P

A

E

A

G

L

G

G

M

M

E

K

E

G

A

A

R

L

D

E

L

A

A

V

D

N

A

R

M

I

G

V

E

S

R

N

G

N

E

P

G

D

K

A

V

V

-

S

L

A

N

N

Q

Q

F

F

A

A

N

T

P

K

D

Y

N

N

P

A

Q

Q

A

I

H

H

Y

E

E

E

G

T

T

T

G

G

P

P

E

E

I

I

W

W

R

R

D

Q

T

T

K

K

G

G

T

H

I

V

T

D

H

C

F

F

V

V

S

A

S

G

M

V

G
|
G

T
|
T

T
x
G

G

G

T
|
T

I

I

M

T

G

G

V

V

S

A

K

R

F

Y

F

L

K

K

E

S

K

V

K

G

P

C

A

G

I

A

Q

T

I

I

V

F

G

A

V

V

Q

E

P

P

V

A

E

E

G

S

A

P

K

V

I

L

P

-

G

-

I

-

R

-

W

-

P

-

E

S

Y

F

L

V

P

P

K

E

I

V

Y

F

E

E

P

A

S

A

R

L

V

V

D

D

R

E

Q

V

L

I

D

Q

V

V

T

S

Q

G

Q

D

E

E

A

A

E

I

E

D

T

T

T

A

R

Q

R

K

L

L

A

P

A

R

E

T

E

D

G

G

I

I

F

F

C

C

G

G

I

F

S
|
S

S

G

G

G

G

A

A

N

V

V

A

Y

A

A

L

L

R

Q

L

I

S

A

A

K

-

R

-

P

E

E

V

M

E

A

N

G

A

K

V

T

I

I

V

V

T

T

I

V

V

I

C
x
P

D
x
S

R
x
Y

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

A

V

L

F

Y

binding o-acetylserine: T76 (= T77), G78 (= G79), N79 (= N80), T80 (= T81)
binding pyridoxal-5'-phosphate: V47 (= V49), K48 (= K50), N79 (= N80), G183 (= G183), T184 (= T184), G185 (≠ T185), T187 (= T187), S256 (= S264), P283 (≠ C289), S284 (≠ D290)
binding alpha-D-ribofuranose: C40 (≠ G42), E41 (≠ N43), N42 (= N44), P43 (= P45), A45 (≠ G47), Y285 (≠ R291)

Q93244 Cysteine synthase 1; O-acetylserine (thiol)-lyase 1; OAS-TL; EC 2.5.1.47 from Caenorhabditis elegans (see 2 papers)
43% identity, 95% coverage: 15:301/303 of query aligns to 13:310/341 of Q93244

query
sites
Q93244

A

A

C

L

V

I

G

G

N

N

T

T

P

P

L

M

V

V

H

Y

L

I

Q

N

R

K

L

L

P

T

G

K

D

G

T

L

R

P

N

G

V

T

I

V

L

A

A

V

K

K

L

I

E

E

G

Y

N

M

N

N

P

P

A

A

G

G

S

S

V

V

K

K

D

D

R

R

P

I

A

G

L

A

S

A

M

M

I

L

Q

A

H

A

A

A

Q

E

E

K

R

D

G

G

E

T

I

V

K

I

P

P

G

G

-

V

D

T

R

T

L

L

I

I

E

E

A
x
P

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

A

L

L

A

A

M

F

V

V

A
|
A

T

A

I

A

K

K

G

G

Y

Y

R

R

M

C

T

I

L

V

I

T

M

M

P

P

E

A

N

S

M

M

S

S

V

G

E

E

R

R

A

T

V

L

M

L

K

K

A

A

F

Y

G

G

A

S

E

E

I

V

L

V

L

L

V

T

S

D

P

P

E

A

G

K

G

G

M

M

E

K

E

G

A

A

R

I

D

D

L

M

A

A

D

N

A

Q

M

L

G

K

E

E

R

N

G

I

E

P

G

G

K
x
S

V

I

-

I

L

L

N

A

Q

Q

F

F

A

D

N

N

P

P

D

N

N

N

P

P

Q

L

A

V

H

H

Y

Y

E

Q

G

T

T

T

G

G

P

P

E

E

I

I

W

W

R

R

D

Q

T

T

K

K

G

G

T

T

I

V

T

D

H

A

F

V

V

V

S

F

S
x
G

M

V

G
|
G

T

T

T

G

G

G

T

T

I

I

M

T

G

G

V

V

S

G

K

R

F

Y

F

L

K

Q

E

E

K

Q

K

N

P

P

A

G

I

V

Q

R

I

V

V

F

G

A

V

V

Q

E

P

P

V

E

E

E

G

S

A

A

K

I

I

L

P

S

G

G

I

-

R

R

R

P

W

A

P

G

P

P

E

H

Y

K

L

I

P

Q

K

G

I

I

-
x
G

-

A

-

G

Y

F

E

A

P

P

S

A

R

V

V

L

D

D

R

T

Q

K

L

I

-

Y

-

E

D

D

V

V

T

I

Q

R

Q

I

E

H

A

S

E

D

E

E

T

A

-

I

-

V

-

M

T

A

R

Q

R
|
R

L

L

A

S

A

Y

E

E

G

G

I

L

F

L

C

G

G

G

I

I

S

S

S
|
S

G

G

G

A

A

N

V

V

A

A

L

L

R

Q

L

L

S

A

A

A

-

R

-

P

E

E

V

M

E

A

N

G

A

K

V

L

I

I

V

V

T
|
T

I

C

V

L

C

P

D

S

R

C

G

G

D

E

R

R

Y

Y

L

M

S

T

T

S

G

P

V

L

F

Y

P75 (≠ A76) mutation to L: In n5537; severe loss of protein stability.
A88 (= A89) mutation to V: In n5522; severe loss of protein stability.
S144 (≠ K145) mutation to F: In mr26; susceptible to high levels of hydrogen sulfide.
G181 (≠ S181) mutation to E: In n5521 and mr23; severe loss of protein stability. Susceptible to high levels of hydrogen sulfide.
G183 (= G183) mutation to R: In n5515; severe loss of protein stability.
G229 (vs. gap) mutation to E: In mr33; susceptible to high levels of hydrogen sulfide.
R259 (= R252) mutation to K: In n5519; no loss of protein stability. No effect on enzyme activity.
S272 (= S265) mutation to F: In mr29; susceptible to high levels of hydrogen sulfide.
T295 (= T286) mutation to I: In mr39; susceptible to high levels of hydrogen sulfide.

1z7yA Crystal structure of the arabidopsis thaliana o-acetylserine sulfhydrylase k46a mutant (see paper)
43% identity, 95% coverage: 16:303/303 of query aligns to 10:308/320 of 1z7yA

query
sites
1z7yA

C

L

V

I

G

G

N

N

T

T

P

P

L

L

V

V

H

Y

L

L

Q

N

R

N

L

V

P

A

G

E

D

G

T

C

R

V

N

G

V

R

I

V

L

A

A

A

K

K

L

L

E

E

G

M

N

M

N

E

P

P

A

C

G

S

S

S

V

V

K
x
A

D

D

R

R

P

I

A

G

L

F

S

S

M

M

I

I

Q

S

H

D

A

A

Q

E

E

K

R

K

G

G

E

L

I

I

K

K

P

P

G

G

D

E

R

S

-

V

L

L

I

I

E

E

A

P

T

T

S

S

G
|
G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

M

F

V

T

A

A

T

A

I

A

K

K

G

G

Y

Y

R

K

M

L

T

I

L

I

I

T

M

M

P

P

E

A

N

S

M

M

S

S

V

T

E

E

R

R

A

I

V

I

M

L

K

L

A

A

F

F

G

G

A

V

E

E

I

L

L

V

L

L

V

T

S

D

P

P

E

A

G

K

G

G

M

M

E

K

E

G

A

A

R

I

D

A

L

K

A

A

D

E

-

E

A

I

M

L

G

A

E

K

R

T

G

P

E

N

G

G

K

Y

V

M

L

L

N

Q

Q
|
Q

F

F

A

E

N

N

P

P

D

A

N

N

P

P

Q

K

A

I

H

H

Y

Y

E

E

G

T

T

T

G

G

P

P

E

E

I

I

W

W

R

K

D

G

T

T

K

G

G

G

T

K

I

I

T

D

H

G

F

F

V

V

S

S

S

G

M
x
I

G
|
G

T
|
T

T
x
G

G

G

T
|
T

I

I

M

T

G

G

V

A

S

G

K

K

F

Y

F

L

K

K

E

E

K

Q

K

N

P

A

A

N

I

V

Q

K

I

L

V

Y

G

G

V

V

Q

E

P

P

V

V

E

E

G

S

A

A

K

I

I

L

P

S

G

G

I

G

R

K

R

P

W

G

P

P

P

H

E

K

-

I

-

Q

-
x
G

-

I

-

G

-

A

-

G

Y

F

L

I

P

P

K

S

I

V

Y

L

E

N

P

V

S

D

R

L

V

I

D

D

R

E

Q

V

L

V

D

Q

V

V

T

S

Q

S

Q

D

E

E

A

S

E

I

E

D

T

M

T

A

R

R

R

Q

L

L

A

A

A

L

E

K

E

E

G

G

I

L

F

L

C

V

G

G

I

I

S
|
S

S

S

G

G

G

A

A

A

V

A

A

A

L

I

R

K

L

L

S

A

A

Q

E

R

V

P

E

E

N

N

A

A

-

G

-

K

V

L

I

F

V

V

T

A

I

I

V

F

C
x
P

D
x
S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

V

V

L

F

F

P

D

A

A

active site: A44 (≠ K50), S267 (= S264)
binding n-[(3-hydroxy-2-methyl-5-{[(trihydroxyphosphoranyl)oxy]methyl}pyridin-4-yl)methylene]methionine: G74 (= G79), N75 (= N80), T76 (= T81), Q145 (= Q149), I178 (≠ M182), G179 (= G183), T180 (= T184), G181 (≠ T185), T183 (= T187), G223 (vs. gap), S267 (= S264), P294 (≠ C289), S295 (≠ D290)

P0ABK5 Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; S-carboxymethylcysteine synthase; Sulfate starvation-induced protein 5; SSI5; EC 2.5.1.47; EC 4.5.1.5 from Escherichia coli (strain K12) (see 5 papers)
44% identity, 94% coverage: 16:301/303 of query aligns to 11:312/323 of P0ABK5

query
sites
P0ABK5

C

T

V

I

G

G

N

H

T

T

P

P

L

L

V

V

H

R

L

L

Q

N

R

R

L

I

P

-

G

G

D

N

T

G

R

R

N

-

V

-

I

I

L

L

A

A

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K42 (= K50) modified: N6-(pyridoxal phosphate)lysine; mutation to A: Still stimulates tRNase activity of CdiA-CT in vitro and in vivo.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

2q3dA 2.2 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) from mycobacterium tuberculosis in complex with the reaction intermediate alpha-aminoacrylate (see paper)
42% identity, 95% coverage: 13:301/303 of query aligns to 7:305/306 of 2q3dA

query
sites
2q3dA

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x
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F

active site: K44 (= K50), S266 (= S264), P293 (≠ C289)
binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-propionic acid: K44 (= K50), T71 (= T77), S72 (= S78), N74 (= N80), T75 (= T81), Q144 (= Q149), V177 (≠ M182), G178 (= G183), T179 (= T184), G180 (≠ T185), T182 (= T187), G222 (vs. gap), I223 (vs. gap), S266 (= S264), P293 (≠ C289), D294 (= D290)

P9WP55 O-acetylserine sulfhydrylase; OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase; EC 2.5.1.47 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
42% identity, 95% coverage: 13:301/303 of query aligns to 7:305/310 of P9WP55

query
sites
P9WP55

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A