SitesBLAST
Comparing WP_022950095.1 NCBI__GCF_000421465.1:WP_022950095.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5ve7A Crystal structure of utp-glucose-1-phosphate uridylyltransferase from burkholderia ambifaria in complex with utp
62% identity, 99% coverage: 1:286/289 of query aligns to 1:281/282 of 5ve7A
- binding uridine 5'-triphosphate: P9 (= P9), V10 (= V10), A11 (= A11), G12 (= G12), G14 (= G14), T15 (= T15), R16 (= R16), K26 (= K26), E27 (= E27), Q104 (= Q104), A107 (= A107), G109 (= G109), A113 (= A113)
5i1fA Crystal structure of utp-glucose-1-phosphate uridylyltransferase from burkholderia vietnamiensis in complex with uridine-5'-diphosphate- glucose
62% identity, 98% coverage: 1:284/289 of query aligns to 3:285/290 of 5i1fA
- binding uridine-5'-diphosphate-glucose: P11 (= P9), A13 (= A11), G14 (= G12), K28 (= K26), E29 (= E27), Q106 (= Q104), A109 (= A107), L110 (= L108), G111 (= G109), L112 (= L110), A115 (= A113), D135 (= D133), Y172 (= Y171), G173 (= G172), E192 (= E191), K193 (= K192), V205 (= V204)
2ux8G Crystal structure of sphingomonas elodea atcc 31461 glucose-1- phosphate uridylyltransferase in complex with glucose-1-phosphate. (see paper)
53% identity, 97% coverage: 3:283/289 of query aligns to 5:282/288 of 2ux8G
8f73E Crystal structure of pseudomonas aeruginosa udp-glucose phosphorylase in complex with udp-glucose (see paper)
53% identity, 91% coverage: 3:266/289 of query aligns to 8:271/281 of 8f73E
- binding uridine-5'-diphosphate-glucose: P14 (= P9), A16 (= A11), G17 (= G12), K31 (= K26), E32 (= E27), Q108 (= Q104), G113 (= G109), L114 (= L110), A117 (= A113), L134 (= L130), D137 (= D133), E196 (= E191), K197 (= K192), I209 (≠ V204)
6knlA Uridine and triphosphate-bound ugpase from acinetobacter baumannii
48% identity, 99% coverage: 3:288/289 of query aligns to 2:290/290 of 6knlA
- binding triphosphate: G13 (= G14), T14 (= T15), R15 (= R16), K79 (≠ R80), K81 (= K82)
- binding uridine: P8 (= P9), G11 (= G12), K25 (= K26), Q103 (= Q104), P106 (≠ A107), G108 (= G109), P130 (≠ A131), D131 (= D132)
6k8dA Udp-glucose pyrophosphorylase with upg from acinetobacter baumanii
48% identity, 99% coverage: 3:288/289 of query aligns to 2:290/290 of 6k8dA
- binding uridine-5'-diphosphate-glucose: P8 (= P9), A10 (= A11), G11 (= G12), Q103 (= Q104), P106 (≠ A107), G108 (= G109), L109 (= L110), A112 (= A113), L129 (= L130), D131 (= D132), E193 (= E191), V206 (= V204)
6ikzB Udp-glucose pyrophosphorylase from acinetobacter baumanii
47% identity, 99% coverage: 3:288/289 of query aligns to 2:285/285 of 6ikzB
- binding uridine 5'-triphosphate: P8 (= P9), V9 (= V10), A10 (= A11), G11 (= G12), L12 (= L13), G13 (= G14), T14 (= T15), R15 (= R16), K25 (= K26), Q103 (= Q104), P106 (≠ A107), G108 (= G109), D131 (= D132)
3jukD The crystal structure of udp-glucose pyrophosphorylase complexed with udp-glucose (see paper)
48% identity, 91% coverage: 3:266/289 of query aligns to 2:257/264 of 3jukD
- binding magnesium ion: T14 (= T15), R15 (= R16)
- binding uridine-5'-diphosphate-glucose: P8 (= P9), A10 (= A11), G11 (= G12), E26 (= E27), Q94 (= Q104), M97 (≠ A107), G99 (= G109), L100 (= L110), A103 (= A113), L120 (= L130), D123 (= D133), Y162 (= Y171), G163 (= G172), E182 (= E191), K183 (= K192), V195 (= V204)
3jukA The crystal structure of udp-glucose pyrophosphorylase complexed with udp-glucose (see paper)
48% identity, 91% coverage: 3:266/289 of query aligns to 2:257/265 of 3jukA
- binding uridine-5'-diphosphate-glucose: A10 (= A11), G11 (= G12), E26 (= E27), Q94 (= Q104), M97 (≠ A107), G99 (= G109), L100 (= L110), A103 (= A113), D123 (= D133), Y162 (= Y171), G163 (= G172), E182 (= E191), K183 (= K192), V195 (= V204)
2ux8A Crystal structure of sphingomonas elodea atcc 31461 glucose-1- phosphate uridylyltransferase in complex with glucose-1-phosphate. (see paper)
47% identity, 97% coverage: 3:283/289 of query aligns to 5:249/255 of 2ux8A
8b6dA Crystal structure of udp-glucose pyrophosphorylase from thermocrispum agreste dsm 44070 in complex with udp
47% identity, 97% coverage: 6:284/289 of query aligns to 5:284/291 of 8b6dA
- binding uridine-5'-diphosphate: P8 (= P9), A10 (= A11), G11 (= G12), L12 (= L13), G13 (= G14), T14 (= T15), R15 (= R16), K25 (= K26), Q102 (= Q104), A105 (= A107), G107 (= G109), A111 (= A113)
8b68A Crystal structure of udp-glucose pyrophosphorylase from thermocrispum agreste dsm 44070 in complex with udp-glucose
46% identity, 97% coverage: 6:284/289 of query aligns to 5:279/286 of 8b68A
- binding uridine-5'-diphosphate-glucose: P8 (= P9), A10 (= A11), G11 (= G12), Q102 (= Q104), A105 (= A107), G107 (= G109), A111 (= A113), L130 (= L130), P131 (≠ A131), D133 (= D133), A203 (≠ V204), G205 (= G206)
2pa4B Crystal structure of udp-glucose pyrophosphorylase from corynebacteria glutamicum in complex with magnesium and udp-glucose (see paper)
41% identity, 99% coverage: 3:287/289 of query aligns to 3:289/299 of 2pa4B
- binding uridine-5'-diphosphate-glucose: P9 (= P9), A10 (≠ V10), A11 (= A11), G12 (= G12), E27 (= E27), Q103 (= Q104), P106 (≠ A107), G108 (= G109), L109 (= L110), L131 (= L130), P132 (≠ A131), D134 (= D133), Y170 (= Y171), G171 (= G172), E192 (= E191), K193 (= K192), A205 (≠ V204)
4ecmA 2.3 angstrom crystal structure of a glucose-1-phosphate thymidylyltransferase from bacillus anthracis in complex with thymidine-5-diphospho-alpha-d-glucose and pyrophosphate (see paper)
25% identity, 88% coverage: 5:258/289 of query aligns to 4:224/245 of 4ecmA
- binding 2'deoxy-thymidine-5'-diphospho-alpha-d-glucose: L8 (≠ P9), G10 (≠ A11), H26 (≠ E27), Q82 (= Q104), A85 (= A107), G87 (= G109), I88 (≠ L110), L108 (= L130), D110 (= D132), F144 (≠ Y171), G145 (= G172), E159 (≠ P193), K160 (= K194), V170 (= V204)
- binding pyrophosphate 2-: T12 (≠ L13), G13 (= G14), S14 (≠ T15), R15 (= R16), K25 (= K26)
3hl3A 2.76 angstrom crystal structure of a putative glucose-1-phosphate thymidylyltransferase from bacillus anthracis in complex with a sucrose.
25% identity, 88% coverage: 5:258/289 of query aligns to 3:223/246 of 3hl3A
2ggqA Complex of hypothetical glucose-1-phosphate thymidylyltransferase from sulfolobus tokodaii
28% identity, 88% coverage: 5:257/289 of query aligns to 2:210/401 of 2ggqA
- active site: R13 (= R16)
- binding thymidine-5'-triphosphate: L6 (≠ P9), A8 (= A11), G9 (= G12), S10 (≠ L13), G11 (= G14), E12 (≠ T15), R13 (= R16), K23 (= K26), Q73 (≠ R103), G79 (= G109), A83 (= A113), R179 (≠ G226), E181 (= E228)
Sites not aligning to the query:
Q975F9 Bifunctional sugar-1-phosphate nucleotidylyltransferase/acetyltransferase; EC 2.7.7.24; EC 2.7.7.9; EC 2.7.7.83; EC 2.7.7.23; EC 2.3.1.276; EC 2.3.1.157 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 3 papers)
28% identity, 88% coverage: 5:257/289 of query aligns to 2:210/401 of Q975F9
- AGSGER 8:13 (≠ AGLGTR 11:16) binding a ribonucleoside 5'-triphosphate
- Q73 (≠ R103) binding a ribonucleoside 5'-triphosphate
- G79 (= G109) binding a ribonucleoside 5'-triphosphate
- T80 (≠ L110) mutation T->A,G,Q: Increases both GlcNAc-1-P UTase and Glc-1-P UTase activities.; mutation T->D,H: Decrease in GlcNAc-1-P UTase activity but increase in Glc-1-P UTase activity.; mutation T->E,K,L,M,R,W,Y: Strong decrease in GlcNAc-1-P UTase activity and loss of Glc-1-P UTase activity.; mutation T->F,I: Loss of both GlcNAc-1-P UTase and Glc-1-P UTase activities.; mutation T->N,S: Strong increase in GlcNAc-1-P UTase activity and decrease in Glc-1-P UTase activity.; mutation to N: Loss of GlcNAc-1-P UTase activity; when associated with V-97.
- Y97 (≠ L130) mutation Y->A,D,F,G,I,K,T,V: Increases GlcNAc-1-P UTase activity. Decreases Glc-1-P UTase activity.; mutation Y->C,E,P,R,W: Decreases GlcNAc-1-P UTase and Glc-1-P UTase activities.; mutation Y->H,L,M,N,Q,S: Increases GlcNAc-1-P UTase and Glc-1-P UTase activities.; mutation to V: Loss of GlcNAc-1-P UTase activity; when associated with N-80.
- E146 (= E191) mutation E->A,C,F,G,I,K,L,M,P,Q,R,V,W,Y: Loss of both GlcNAc-1-P UTase and Glc-1-P UTase activities.; mutation E->D,N: Decrease in GlcNAc-1-P UTase and Glc-1-P UTase activities.; mutation E->H,S,T: Decrease in GlcNAc-1-P UTase activity and loss of Glc-1-P UTase activity.
Sites not aligning to the query:
- 308 H→A: Strong decrease in GalN-1-P AcTase activity and almost loss of GlcN-1-P AcTase activity.
- 311 Y→A: Strong decrease in GalN-1-P AcTase activity and increase in GlcN-1-P AcTase activity.
- 331 N→A: Strong decrease in GalN-1-P AcTase activity and decrease in GlcN-1-P AcTase activity.
- 337 K→A: Slight decrease in GalN-1-P AcTase activity and increase in GlcN-1-P AcTase activity.
- 340 K→A: Decrease in GalN-1-P AcTase activity and increase in GlcN-1-P AcTase activity.
- 391:401 mutation Missing: No change in GlcNAc-1-P UTase activity. Shows 38% less GalN-1-P AcTase activity than the wild-type, but increases GlcN-1-P AcTase activity 16.8 times. Significantly affects the thermostability of the entire protein.
- 397:401 mutation Missing: No change in GlcNAc-1-P UTase activity. Shows 20% less GalN-1-P AcTase activity than the wild-type, but increases GlcN-1-P AcTase activity 4.8 times. Does not affect thermostability.
5z09A St0452(y97n)-utp binding form (see paper)
29% identity, 88% coverage: 5:257/289 of query aligns to 2:210/401 of 5z09A
- binding uridine 5'-triphosphate: L6 (≠ P9), A7 (≠ V10), A8 (= A11), G9 (= G12), S10 (≠ L13), G11 (= G14), E12 (≠ T15), R13 (= R16), Q73 (≠ R103), G79 (= G109), G98 (≠ D132), D99 (= D133)
4ho4A Crystal structure of glucose 1-phosphate thymidylyltransferase from aneurinibacillus thermoaerophilus complexed with thymidine and glucose-1-phosphate
26% identity, 82% coverage: 5:240/289 of query aligns to 2:206/289 of 4ho4A
Sites not aligning to the query:
1h5sB Thymidylyltransferase complexed with tmp (see paper)
27% identity, 81% coverage: 1:233/289 of query aligns to 1:202/291 of 1h5sB
- binding thymidine-5'-phosphate: L9 (≠ P9), G11 (≠ A11), G12 (= G12), Q83 (= Q104), P86 (≠ A107), D87 (≠ L108), G88 (= G109), D111 (= D132), Y115 (≠ E136), G116 (≠ N137), H117 (≠ N138), D118 (≠ G139)
Sites not aligning to the query:
Query Sequence
>WP_022950095.1 NCBI__GCF_000421465.1:WP_022950095.1
MKIKKAVFPVAGLGTRFLPATKASPKEMLPVVDKPLIQYAVEEAAAAGIELMVFVTGRNK
TAISNHFDKAYELETELEHRGKIDKLELVRNIPPAGVNCVYIRQAEALGLGHAVLCAKPI
IGNEPFAVLLADDLIENNGRGCMEEMVEIYESHGRSVLAVEEIPLDASESYGVVAASEVV
DNVTRVEGIVEKPKPEEAPSNLGVVGRYILTPRIFDLLETVGRGAGGEIQLTDAIAQLLE
HELVYAHRFSGKRYDCGAKLGYLEATVDHALQHPELAEDFRAYLKSLNL
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory