SitesBLAST
Comparing WP_023430324.1 NCBI__GCF_000496075.1:WP_023430324.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
O58389 L-threonine 3-dehydrogenase; L-ThrDH; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 2 papers)
38% identity, 82% coverage: 32:350/388 of query aligns to 19:322/348 of O58389
- C42 (= C55) binding Zn(2+)
- T44 (= T57) mutation to A: Total loss of enzymatic activity.
- H67 (= H84) binding Zn(2+)
- E68 (= E85) binding Zn(2+)
- C97 (= C114) binding Zn(2+)
- C100 (= C117) binding Zn(2+)
- C103 (= C120) binding Zn(2+)
- C111 (= C128) binding Zn(2+)
- E152 (≠ V180) mutation E->A,Q: Almost complete loss of enzymatic activity.; mutation to C: 120-fold decrease in catalytic efficiency.; mutation to D: Shows 3-fold higher turnover rate and reduced affinities toward L-threonine and NAD(+), compared to wild-type.; mutation to K: Total loss of enzymatic activity.
- L179 (≠ I209) binding NAD(+)
- E199 (≠ G229) binding NAD(+); mutation to A: Large decrease in affinity for NAD(+).
- R204 (= R234) binding NAD(+); mutation to A: Large decrease in affinity for NAD(+).
- LGL 266:268 (≠ AAF 296:298) binding NAD(+)
- IT 291:292 (≠ IR 320:321) binding NAD(+)
- R294 (≠ E323) mutation to A: 4000-fold decrease in catalytic efficiency.
2dfvA Hyperthermophilic threonine dehydrogenase from pyrococcus horikoshii (see paper)
38% identity, 82% coverage: 32:350/388 of query aligns to 17:320/346 of 2dfvA
- active site: C40 (= C55), G41 (≠ A56), T42 (= T57), H45 (≠ E60), H65 (= H84), E66 (= E85), C95 (= C114), C98 (= C117), C101 (= C120), C109 (= C128), K113 (≠ R143), P151 (≠ T181), A155 (= A185)
- binding nicotinamide-adenine-dinucleotide: G175 (= G207), P176 (= P208), L177 (≠ I209), E197 (≠ G229), P198 (≠ T230), R202 (= R234), F241 (≠ C273), S242 (= S274), A244 (= A276), L264 (≠ A296), G265 (≠ A297), L266 (≠ F298), I289 (= I320), T290 (≠ R321)
- binding zinc ion: C95 (= C114), C101 (= C120), C109 (= C128)
Sites not aligning to the query:
3gfbA L-threonine dehydrogenase (tktdh) from the hyperthermophilic archaeon thermococcus kodakaraensis (see paper)
35% identity, 86% coverage: 32:363/388 of query aligns to 17:334/347 of 3gfbA
- active site: C40 (= C55), G41 (≠ A56), T42 (= T57), H45 (≠ E60), H65 (= H84), E66 (= E85), C95 (= C114), C98 (= C117), C101 (= C120), C109 (= C128), K113 (≠ R143), P151 (≠ T181), A155 (= A185)
- binding nicotinamide-adenine-dinucleotide: G173 (= G205), G175 (= G207), P176 (= P208), L177 (≠ I209), S196 (≠ T228), E197 (≠ G229), P198 (≠ T230), R202 (= R234), F241 (≠ C273), S242 (= S274), A244 (= A276), L264 (= L295), G265 (≠ A296), L266 (≠ A297), I289 (= I320), T290 (≠ R321)
Sites not aligning to the query:
Q5JI69 L-threonine 3-dehydrogenase; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1)) (see paper)
35% identity, 86% coverage: 32:363/388 of query aligns to 19:336/350 of Q5JI69
- L179 (≠ I209) binding NAD(+)
- E199 (≠ G229) binding NAD(+)
- R204 (= R234) binding NAD(+)
- LGL 266:268 (≠ LAA 295:297) binding NAD(+)
- IT 291:292 (≠ IR 320:321) binding NAD(+)
P07913 L-threonine 3-dehydrogenase; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Escherichia coli (strain K12) (see paper)
32% identity, 86% coverage: 30:364/388 of query aligns to 13:330/341 of P07913
- C38 (= C55) mutation to D: Shows only 1% of wild-type catalytic activity. This mutant can be stimulated to the wild-type activity level after incubation with Zn(+).; mutation to S: Loss of catalytic activity. This mutant cannot be stimulated to the wild-type activity level after incubation with Zn(+).
5kiaA Crystal structure of l-threonine 3-dehydrogenase from burkholderia thailandensis
32% identity, 88% coverage: 19:359/388 of query aligns to 2:323/339 of 5kiaA
- active site: C37 (= C55), G38 (≠ A56), T39 (= T57), H42 (≠ E60), H61 (= H84), E62 (= E85), C91 (= C114), C94 (= C117), C97 (= C120), C105 (= C128), V109 (≠ R143), P147 (≠ T181), A151 (= A185)
- binding calcium ion: D146 (≠ V180), N150 (≠ T184), E288 (≠ G324)
- binding zinc ion: C91 (= C114), C94 (= C117), C97 (= C120), C105 (= C128)
Sites not aligning to the query:
2ejvA Crystal structure of threonine 3-dehydrogenase complexed with NAD+
33% identity, 87% coverage: 24:359/388 of query aligns to 7:326/343 of 2ejvA
- active site: C38 (= C55), G39 (≠ A56), T40 (= T57), H43 (≠ E60), H63 (= H84), E64 (= E85), C93 (= C114), C96 (= C117), C99 (= C120), C107 (= C128), Q111 (≠ G132), P149 (≠ T181), A153 (= A185)
- binding nicotinamide-adenine-dinucleotide: G172 (= G205), G174 (= G207), P175 (= P208), I176 (= I209), S195 (≠ T228), D196 (≠ G229), P197 (≠ T230), R201 (= R234), F238 (≠ C273), S239 (= S274), N241 (≠ A276), A244 (= A279), L261 (≠ A296), G262 (≠ A297), I263 (≠ F298)
- binding zinc ion: C38 (= C55), H63 (= H84), E64 (= E85), C96 (= C117), C99 (= C120), C107 (= C128)
Sites not aligning to the query:
2dq4A Crystal structure of threonine 3-dehydrogenase
33% identity, 87% coverage: 24:359/388 of query aligns to 7:326/343 of 2dq4A
- active site: C38 (= C55), G39 (≠ A56), T40 (= T57), H43 (≠ E60), H63 (= H84), E64 (= E85), C93 (= C114), C96 (= C117), C99 (= C120), C107 (= C128), Q111 (≠ G132), P149 (≠ T181), A153 (= A185)
- binding zinc ion: C38 (= C55), H63 (= H84), E64 (= E85), C93 (= C114), C96 (= C117), C107 (= C128)
Sites not aligning to the query:
Q5SKS4 L-threonine 3-dehydrogenase; TDH; EC 1.1.1.103 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
33% identity, 87% coverage: 24:359/388 of query aligns to 7:326/343 of Q5SKS4
- C38 (= C55) binding Zn(2+)
- H63 (= H84) binding Zn(2+)
- E64 (= E85) binding Zn(2+)
- C93 (= C114) binding Zn(2+)
- C96 (= C117) binding Zn(2+)
- C99 (= C120) binding Zn(2+)
- C107 (= C128) binding Zn(2+)
- I176 (= I209) binding NAD(+)
- D196 (≠ G229) binding NAD(+)
- R201 (= R234) binding NAD(+)
- LGI 261:263 (≠ AAF 296:298) binding NAD(+)
- IAG 286:288 (≠ IRG 320:322) binding NAD(+)
P12311 Alcohol dehydrogenase; ADH-T; EC 1.1.1.1 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see paper)
33% identity, 76% coverage: 27:319/388 of query aligns to 12:283/337 of P12311
- C38 (= C55) mutation to S: No activity.
- T40 (= T57) mutation to A: No activity.; mutation to S: Little decrease in activity.
- H43 (≠ E60) mutation to A: No activity.; mutation to R: Higher level of activity at pH 9.
1rjwA Crystal structure of NAD(+)-dependent alcohol dehydrogenase from bacillus stearothermophilus strain lld-r (see paper)
34% identity, 78% coverage: 19:319/388 of query aligns to 1:283/339 of 1rjwA
- active site: C38 (= C55), H39 (≠ A56), T40 (= T57), H43 (≠ E60), H61 (= H84), E62 (= E85), C92 (= C114), C95 (= C117), C98 (= C120), C106 (= C128), K110 (≠ A144), C148 (≠ T181), T152 (≠ A185)
- binding trifluoroethanol: T40 (= T57), C148 (≠ T181)
- binding zinc ion: C38 (= C55), H61 (= H84), C92 (= C114), C95 (= C117), C98 (= C120), C106 (= C128)
Sites not aligning to the query:
3piiA Crystal structure of mutant of ht- alcohol dehydrogenase with substrate analogue butyramide
34% identity, 78% coverage: 19:319/388 of query aligns to 1:283/337 of 3piiA
- active site: C38 (= C55), H39 (≠ A56), T40 (= T57), H43 (≠ E60), H61 (= H84), E62 (= E85), C92 (= C114), C95 (= C117), C98 (= C120), C106 (= C128), K110 (≠ A144), C148 (≠ T181), T152 (≠ A185)
- binding butyramide: T40 (= T57), H61 (= H84), W87 (vs. gap), C148 (≠ T181)
- binding zinc ion: C38 (= C55), H61 (= H84), E62 (= E85), C92 (= C114), C95 (= C117), C98 (= C120), C106 (= C128), C148 (≠ T181)
Sites not aligning to the query:
5vm2A Crystal structure of eck1772, an oxidoreductase/dehydrogenase of unknown specificity involved in membrane biogenesis from escherichia coli
28% identity, 91% coverage: 23:375/388 of query aligns to 7:344/347 of 5vm2A
- active site: C39 (= C55), G40 (≠ A56), S41 (≠ T57), H44 (≠ E60), H65 (= H84), E66 (= E85), C95 (= C114), C98 (= C117), C101 (= C120), C109 (= C128), D113 (≠ N134), P153 (≠ T181), G157 (≠ A185), K340 (= K371)
- binding magnesium ion: H65 (= H84), E66 (= E85), E152 (≠ V180)
- binding zinc ion: C95 (= C114), C98 (= C117), C101 (= C120), C109 (= C128)
5ylnA Zinc dependent alcohol dehydrogenase 2 from streptococcus pneumonia - apo form
28% identity, 93% coverage: 18:377/388 of query aligns to 4:344/348 of 5ylnA