SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_023430621.1 NCBI__GCF_000496075.1:WP_023430621.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P81601 Alcohol dehydrogenase class-3 chain L; Alcohol dehydrogenase class-III chain L; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.1; EC 1.1.1.-; EC 1.1.1.284 from Gadus morhua (Atlantic cod) (see 2 papers)
57% identity, 100% coverage: 1:375/375 of query aligns to 7:375/375 of P81601

query
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P81601

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Sites not aligning to the query:

1 modified: N-acetylalanine

P80467 Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.1; EC 1.1.1.-; EC 1.1.1.284 from Saara hardwickii (Indian spiny-tailed lizard) (Uromastyx hardwickii) (see paper)
57% identity, 100% coverage: 1:375/375 of query aligns to 5:373/373 of P80467

query
sites
P80467

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Sites not aligning to the query:

1 modified: N-acetylalanine

P81600 Alcohol dehydrogenase class-3 chain H; Alcohol dehydrogenase class-III chain H; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.1; EC 1.1.1.-; EC 1.1.1.284 from Gadus morhua (Atlantic cod) (see 2 papers)
57% identity, 100% coverage: 1:375/375 of query aligns to 7:375/375 of P81600

query
sites
P81600

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Sites not aligning to the query:

1 modified: N-acetylalanine

P12711 Alcohol dehydrogenase class-3; Alcohol dehydrogenase 2; Alcohol dehydrogenase 5; Alcohol dehydrogenase B2; ADH-B2; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.1; EC 1.1.1.-; EC 1.1.1.284 from Rattus norvegicus (Rat) (see 2 papers)
58% identity, 99% coverage: 1:373/375 of query aligns to 6:372/374 of P12711

query
sites
P12711

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L

H

H

Y

F

M

M

G

G

T

T

S

S

T

T

F

F

S

S

N

E

F

Y

T

T

V

V

L

V

P

A

E

D

I

I

A

S

V

V

A

A

K

K

I

I

R

D

E

P

D

S

A

A

P

P

F

L

D

D

K

K

V

V

C

C

Y

L

I

L

G

G

C

C

G

G

V

I

T

S

T

T

G

G

I

Y

G

G

A

A

V

A

I

V

N

N

T

T

A

A

K

K

V

V

E

E

A

P

G

G

A

S

T

T

A

C

V

A

V

V

F

F

G

G

L

L

G

G

I

V

G

G

L

L

N

A

V

V

I

I

Q

M

G

G

L

C

R

K

L

V

A

A

G

G

A

A

D

S

M

R

I

I

G

G

V

I

D

D

L

I

N

N

N

K

D

D

K

K

K

F

E

A

W

K

G

A

E

K

R

E

F

F

G

G

M

A

T

T

H

E

F

C

V

I

N

N

P

P

K

Q

E

D

V

F

G

S

S

K

D

S

L

I

V

Q

P

E

H

V

L

L

V

I

N

E

M

M

T

T

K

D

R

-

G

-

A

-

D

-

Q

-

I

-

G

G

A

V

D

D

Y

F

T

S

F

F

D

E

C

C

T

I

G

G

N

N

V

V

T

K

V

V

M

M

R

R

Q

S

A

A

L

L

E

E

C

A

A

A

H

H

R

K

G

G

W

W

G

G

Q

V

S

S

I

V

V

V

I

V

G

G

V

V

A

A

P

A

A

S

G

G

A

E

E

E

I

I

S

S

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

T

W

W

K

K

G

G

T

T

A

A

F

F

G

G

A

W

R

K

G

S

R

V

T

E

D

S

V

V

P

P

K

K

I

L

V

V

E

S

W

E

Y

Y

M

M

E

S

G

K

K

K

I

I

E

K

I

V

D

D

P

E

M

F

I

V

T

T

H

G

T

N

M

L

P

S

L

F

D

D

D

Q

I

I

N

N

K

K

G

A

F

F

D

D

L

L

M

M

H

H

H

S

G

G

E

N

S

S

I

I

R

R

S

T

V

V

V

L

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine

2fzeA Crystal structure of the binary complex of human glutathione-dependent formaldehyde dehydrogenase with adp-ribose (see paper)
58% identity, 99% coverage: 1:373/375 of query aligns to 5:371/373 of 2fzeA

query
sites
2fzeA

M

I

E

K

S

C

R

K

A

A

V

V

A

A

F

W

E

E

A

A

G

G

K

K

P

P

L

L

E

S

V

I

A

E

T

E

V

I

R

E

L

V

E

A

G

P

P

P

K

K

E

A

G

H

E

E

V

V

L

R

V

I

E

K

I

I

K

I

A

A

T

T

G

A

I

V

C
|
C

H
|
H

T
|
T

D

D

E

A

F
x
Y

T

T

R

L

S

S

G

G

A

A

D

D

P

P

E

E

G

G

I

C

F

F

P

P

A

V

I

I

L

L

G

G

H
|
H

E
|
E

G

G

A

A

G

G

V

I

V

V

V

E

D

S

V

V

G

G

P

E

G

G

V

V

R

T

S

K

L

L

R
x
K

K

A

G

G

D

D

H

T

V

V

I

I

P

P

L

L

Y

Y

T

I

P

P

E

Q

C
|
C

R

G

E

E

C
|
C

P

K

S

F

C
|
C

L

L

S

N

R

P

K

K

T

T

N

N

L

L

C
|
C

T

Q

A

K

I

I

R
|
R

T

V

T

T

Q

Q

G

G

Q

K

G

G

L

L

M

M

P

P

D

D

G

G

T

T

S

S

R

R

F

F

S

T

L

C

D

K

G

G

K

K

P

T

I

I

H

L

H

H

Y

Y

M

M

G

G

T

T

S

S

T

T

F

F

S

S

N

E

F

Y

T

T

V

V

L

V

P

A

E

D

I

I

A

S

V

V

A

A

K
|
K

I

I

R

D

E

P

D

L

A

A

P

P

F

L

D

D

K

K

V

V

C

C

Y

L

I

L

G

G

C
|
C

G

G

V

I

T

S

T
|
T

G

G

I

Y

G

G

A

A

V

A

I

V

N

N

T

T

A

A

K

K

V

L

E

E

A

P

G

G

A

S

T

V

A

C

V

A

V

V

F

F

G

G

L

L

G
|
G

G

G

I
x
V

G

G

L

L

N

A

V

V

I

I

Q

M

G

G

L

C

R

K

L

V

A

A

G

G

A

A

D

S

M

R

I

I

G

G

V

V

D
|
D

L
x
I

N

N

N

K

D

D

K

K

K

F

E

A

W

R

G

A

E

K

R

E

F

F

G

G

M

A

T

T

H

E

F

C

V

I

N

N

P

P

K

Q

E

D

V

F

G

S

S

K

D

P

L

I

V

Q

P

E

H

V

L

L

V

I

N

E

M

M

T

T

K

D

R

-

G

-

A

-

D

-

Q

-

I

-

G

G

A

V

D

D

Y

Y

T

S

F

F

D

E

C

C

T
x
I

G

G

N

N

V

V

T

K

V

V

M

M

R

R

Q

A

A

A

L

L

E

E

C

A

A

C

H

H

R

K

G

G

W

W

G

G

Q

V

S

S

I

V

V

V

I

V

G
|
G

V

V

A

A

P

A

A

S

G

G

A

E

E

E

I

I

S

A

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

T

W

W

K

K

G

G

T

T

A

A

F

F

G

G

A

W

R

K

G

S

R

V

T

E

D

S

V

V

P

P

K

K

I

L

V

V

E

S

W

E

Y

Y

M

M

E

S

G

K

K

K

I

I

E

K

I

V

D

D

P

E

M

F

I

V

T

T

H

H

T

N

M

L

P

S

L

F

D

D

D

E

I

I

N

N

K

K

G

A

F

F

D

E

L

L

M

M

H

H

H

S

G

G

E

K

S

S

I

I

R
|
R

S

T

V

V

active site: C44 (= C40), H45 (= H41), T46 (= T42), Y49 (≠ F45), H66 (= H62), E67 (= E63), C96 (= C92), C99 (= C95), C102 (= C98), C110 (= C106), R114 (= R110), C173 (= C169), T177 (= T173), R368 (= R370)
binding adenosine-5-diphosphoribose: G200 (= G196), V202 (≠ I198), D222 (= D218), I223 (≠ L219), I268 (≠ T270), G292 (= G294)
binding phosphate ion: K83 (≠ R79), K158 (= K154)
binding zinc ion: H66 (= H62), C96 (= C92), C99 (= C95), C102 (= C98), C110 (= C106), C173 (= C169)

1mc5A Ternary complex of human glutathione-dependent formaldehyde dehydrogenase with s-(hydroxymethyl)glutathione and nadh (see paper)
58% identity, 99% coverage: 1:373/375 of query aligns to 5:371/373 of 1mc5A

query
sites
1mc5A

M

I

E

K

S

C

R

K

A

A

V

V

A

A

F

W

E

E

A

A

G

G

K

K

P

P

L

L

E

S

V

I

A

E

T

E

V

I

R

E

L

V

E

A

G

P

P

P

K

K

E

A

G

H

E

E

V

V

L

R

V

I

E

K

I

I

K

I

A

A

T

T

G

A

I

V

C
|
C

H
|
H

T
|
T

D

D

E

A

F
x
Y

T

T

R

L

S

S

G

G

A

A

D

D

P
|
P

E
|
E

G

G

I

C

F

F

P

P

A

V

I

I

L

L

G

G

H
|
H

E
|
E

G

G

A

A

G

G

V

I

V

V

V

E

D

S

V

V

G

G

P

E

G

G

V

V

R

T

S

K

L

L

R

K

K

A

G

G

D

D

H

T

V

V

I

I

P

P

L

L

Y
|
Y

T

I

P

P

E

Q

C
|
C

R

G

E

E

C
|
C

P

K

S

F

C
|
C

L

L

S

N

R

P

K

K

T

T

N

N

L

L

C
|
C

T

Q

A

K

I

I

R
|
R

T

V

T

T

Q

Q

G

G

Q

K

G

G

L

L

M

M

P

P

D

D

G

G

T

T

S

S

R

R

F

F

S

T

L

C

D

K

G

G

K

K

P

T

I

I

H

L

H

H

Y

Y

M

M

G

G

T

T

S

S

T

T

F

F

S

S

N

E

F

Y

T

T

V

V

L

V

P

A

E

D

I

I

A

S

V

V

A

A

K

K

I

I

R

D

E

P

D

L

A

A

P

P

F

L

D

D

K

K

V

V

C

C

Y

L

I

L

G

G

C
|
C

G

G

V

I

T

S

T
|
T

G

G

I

Y

G

G

A

A

V

A

I

V

N

N

T

T

A

A

K

K

V

L

E

E

A

P

G

G

A

S

T

V

A

C

V

A

V

V

F

F

G
|
G

L

L

G

G

G
|
G

I
x
V

G

G

L

L

N

A

V

V

I

I

Q

M

G

G

L

C

R

K

L

V

A

A

G

G

A

A

D

S

M

R

I

I

G

G

V

V

D
|
D

L
x
I

N

N

N

K

D

D

K
|
K

K

F

E

A

W

R

G

A

E

K

R

E

F

F

G

G

M

A

T

T

H

E

F

C

V

I

N

N

P

P

K

Q

E

D

V

F

G

S

S

K

D

P

L

I

V

Q

P

E

H

V

L

L

V

I

N

E

M

M

T

T

K

D

R

-

G

-

A

-

D

-

Q

-

I

-

G

G

A

V

D

D

Y

Y

T

S

F

F

D

E

C
|
C

T
x
I

G

G

N

N

V

V

T

K

V

V

M

M

R

R

Q

A

A

A

L

L

E

E

C

A

A

C

H

H

R

K

G

G

W

W

G

G

Q

V

S

S

I

V

V

V

I
x
V

G
|
G

V
|
V

A

A

P

A

A

S

G

G

A

E

E

E

I

I

S

A

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

T

W

W

K

K

G

G

T

T

A
|
A

F

F

G

G

A

W

R

K

G

S

R

V

T

E

D

S

V

V

P

P

K

K

I

L

V

V

E

S

W

E

Y

Y

M

M

E

S

G

K

K

K

I

I

E

K

I

V

D

D

P

E

M

F

I

V

T

T

H

H

T

N

M

L

P

S

L

F

D

D

D

E

I

I

N

N

K

K

G

A

F

F

D

E

L

L

M

M

H

H

H

S

G

G

E

K

S

S

I

I

R
|
R

S

T

V

V

active site: C44 (= C40), H45 (= H41), T46 (= T42), Y49 (≠ F45), H66 (= H62), E67 (= E63), C96 (= C92), C99 (= C95), C102 (= C98), C110 (= C106), R114 (= R110), C173 (= C169), T177 (= T173), R368 (= R370)
binding 2-amino-4-[1-carboxymethyl-carbamoyl)-2-hydroxymethylsulfanyl-ethylcarbamoyl]-butyric acid: T46 (= T42), P56 (= P52), E57 (= E53), H66 (= H62), Y92 (= Y88), R114 (= R110), C173 (= C169), V293 (= V295)
binding nicotinamide-adenine-dinucleotide: C44 (= C40), H45 (= H41), T46 (= T42), C173 (= C169), T177 (= T173), G198 (= G194), G201 (= G197), V202 (≠ I198), D222 (= D218), I223 (≠ L219), K227 (= K223), C267 (= C269), I268 (≠ T270), V291 (≠ I293), G292 (= G294), V293 (= V295), A317 (= A319), R368 (= R370)
binding zinc ion: C44 (= C40), H66 (= H62), C96 (= C92), C99 (= C95), C102 (= C98), C110 (= C106), C173 (= C169)

1ma0A Ternary complex of human glutathione-dependent formaldehyde dehydrogenase with NAD+ and dodecanoic acid (see paper)
58% identity, 99% coverage: 1:373/375 of query aligns to 5:371/373 of 1ma0A

query
sites
1ma0A

M

I

E

K

S

C

R

K

A

A

V

V

A

A

F

W

E

E

A

A

G

G

K

K

P

P

L

L

E

S

V

I

A

E

T

E

V

I

R

E

L

V

E

A

G

P

P

P

K

K

E

A

G

H

E

E

V

V

L

R

V

I

E

K

I

I

K

I

A

A

T

T

G

A

I

V

C
|
C

H
|
H

T
|
T

D

D

E

A

F
x
Y

T

T

R

L

S

S

G

G

A

A

D

D

P

P

E

E

G

G

I

C

F

F

P

P

A

V

I

I

L

L

G

G

H
|
H

E
|
E

G

G

A

A

G

G

V

I

V

V

V

E

D

S

V

V

G

G

P

E

G

G

V

V

R

T

S

K

L

L

R

K

K

A

G

G

D

D

H

T

V

V

I

I

P

P

L

L

Y

Y

T

I

P

P

E

Q

C
|
C

R

G

E

E

C
|
C

P

K

S

F

C
|
C

L

L

S

N

R

P

K

K

T

T

N

N

L

L

C
|
C

T

Q

A

K

I

I

R
|
R

T

V

T

T

Q

Q

G

G

Q

K

G

G

L

L

M

M

P

P

D

D

G

G

T

T

S

S

R

R

F

F

S

T

L

C

D

K

G

G

K

K

P

T

I

I

H

L

H

H

Y

Y

M

M

G

G

T

T

S

S

T

T

F

F

S

S

N

E

F

Y

T

T

V

V

L

V

P

A

E

D

I

I

A

S

V

V

A

A

K

K

I

I

R

D

E

P

D

L

A

A

P

P

F

L

D

D

K

K

V

V

C

C

Y

L

I

L

G

G

C
|
C

G

G

V

I

T

S

T
|
T

G

G

I

Y

G

G

A

A

V

A

I

V

N

N

T

T

A

A

K

K

V

L

E

E

A

P

G

G

A

S

T

V

A

C

V

A

V

V

F

F

G

G

L

L

G
|
G

G

G

I
x
V

G

G

L

L

N

A

V

V

I

I

Q

M

G

G

L

C

R

K

L

V

A

A

G

G

A

A

D

S

M

R

I

I

G

G

V

V

D
|
D

L
x
I

N

N

N

K

D

D

K

K

K

F

E

A

W

R

G

A

E

K

R

E

F

F

G

G

M

A

T

T

H

E

F

C

V

I

N

N

P

P

K

Q

E

D

V

F

G

S

S

K

D

P

L

I

V

Q

P

E

H

V

L

L

V

I

N

E

M

M

T

T

K

D

R

-

G

-

A

-

D

-

Q

-

I

-

G

G

A

V

D

D

Y

Y

T

S

F

F

D

E

C
|
C

T
x
I

G

G

N

N

V

V

T

K

V

V

M

M

R

R

Q

A

A

A

L

L

E

E

C

A

A

C

H

H

R

K

G

G

W

W

G

G

Q

V

S

S

I

V

V

V

I
x
V

G
|
G

V
|
V

A

A

P

A

A

S

G

G

A

E

E

E

I

I

S

A

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

T

W

W

K

K

G

G

T

T

A
|
A

F
|
F

G

G

A

W

R

K

G

S

R

V

T

E

D

S

V

V

P

P

K

K

I

L

V

V

E

S

W

E

Y

Y

M

M

E

S

G

K

K

K

I

I

E

K

I

V

D

D

P

E

M

F

I

V

T

T

H

H

T

N

M

L

P

S

L

F

D

D

D

E

I

I

N

N

K

K

G

A

F

F

D

E

L

L

M

M

H

H

H

S

G

G

E

K

S

S

I

I

R
|
R

S

T

V

V

active site: C44 (= C40), H45 (= H41), T46 (= T42), Y49 (≠ F45), H66 (= H62), E67 (= E63), C96 (= C92), C99 (= C95), C102 (= C98), C110 (= C106), R114 (= R110), C173 (= C169), T177 (= T173), R368 (= R370)
binding nicotinamide-adenine-dinucleotide: H45 (= H41), T177 (= T173), G200 (= G196), V202 (≠ I198), D222 (= D218), I223 (≠ L219), C267 (= C269), I268 (≠ T270), V291 (≠ I293), G292 (= G294), V293 (= V295), A317 (= A319), F318 (= F320)
binding zinc ion: C44 (= C40), H66 (= H62), E67 (= E63), C96 (= C92), C99 (= C95), C102 (= C98), C110 (= C106), C173 (= C169)

1m6wA Binary complex of human glutathione-dependent formaldehyde dehydrogenase and 12-hydroxydodecanoic acid (see paper)
58% identity, 99% coverage: 1:373/375 of query aligns to 5:371/373 of 1m6wA

query
sites
1m6wA

M

I

E

K

S

C

R

K

A

A

V

V

A

A

F

W

E

E

A

A

G

G

K

K

P

P

L

L

E

S

V

I

A

E

T

E

V

I

R

E

L

V

E

A

G

P

P

P

K

K

E

A

G

H

E

E

V

V

L

R

V

I

E

K

I

I

K

I

A

A

T

T

G

A

I

V

C
|
C

H
|
H

T
|
T

D

D

E

A

F
x
Y

T

T

R

L

S

S

G

G

A

A

D

D

P

P

E

E

G

G

I

C

F

F

P

P

A

V

I

I

L

L

G

G

H
|
H

E
|
E

G

G

A

A

G

G

V

I

V

V

V

E

D

S

V

V

G

G

P

E

G

G

V

V

R

T

S

K

L

L

R

K

K

A

G

G

D

D

H

T

V

V

I

I

P

P

L

L

Y
|
Y

T

I

P

P

E

Q

C
|
C

R

G

E

E

C
|
C

P

K

S

F

C
|
C

L

L

S

N

R

P

K

K

T

T

N

N

L

L

C
|
C

T
x
Q

A

K

I

I

R
|
R

T

V

T

T

Q

Q

G

G

Q

K

G

G

L

L

M

M

P

P

D

D

G

G

T

T

S

S

R

R

F

F

S

T

L

C

D

K

G

G

K

K

P

T

I

I

H

L

H

H

Y

Y

M

M

G

G

T

T

S

S

T

T

F

F

S

S

N

E

F

Y

T

T

V

V

L

V

P

A

E

D

I

I

A

S

V

V

A

A

K

K

I

I

R

D

E

P

D

L

A

A

P

P

F

L

D

D

K

K

V

V

C

C

Y

L

I

L

G

G

C
|
C

G

G

V

I

T

S

T
|
T

G

G

I

Y

G

G

A

A

V

A

I

V

N

N

T

T

A

A

K

K

V

L

E

E

A

P

G

G

A

S

T

V

A

C

V

A

V

V

F

F

G

G

L

L

G

G

I

V

G

G

L

L

N

A

V

V

I

I

Q

M

G

G

L

C

R

K

L

V

A

A

G

G

A

A

D

S

M

R

I

I

G

G

V

V

D

D

L

I

N

N

N

K

D

D

K

K

K

F

E

A

W

R

G

A

E

K

R

E

F

F

G

G

M

A

T

T

H

E

F

C

V

I

N

N

P

P

K

Q

E

D

V

F

G

S

S

K

D

P

L

I

V

Q

P

E

H

V

L

L

V

I

N

E

M

M

T

T

K

D

R

-

G

-

A

-

D

-

Q

-

I

-

G

G

A

V

D

D

Y

Y

T

S

F

F

D

E

C

C

T

I

G

G

N

N

V

V

T

K

V

V

M

M

R

R

Q

A

A

A

L

L

E

E

C

A

A

C

H

H

R

K

G

G

W

W

G

G

Q

V

S

S

I

V

V

V

I

V

G

G

V

V

A

A

P

A

A

S

G

G

A

E

E

E

I

I

S

A

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

T

W

W

K

K

G

G

T

T

A

A

F

F

G

G

A

W

R

K

G

S

R

V

T

E

D

S

V

V

P

P

K

K

I

L

V

V

E

S

W

E

Y

Y

M

M

E

S

G

K

K

K

I

I

E

K

I

V

D

D

P

E

M

F

I

V

T

T

H

H

T

N

M

L

P

S

L

F

D

D

D

E

I

I

N

N

K

K

G

A

F

F

D

E

L

L

M

M

H

H

H

S

G

G

E

K

S

S

I

I

R
|
R

S

T

V

V

active site: C44 (= C40), H45 (= H41), T46 (= T42), Y49 (≠ F45), H66 (= H62), E67 (= E63), C96 (= C92), C99 (= C95), C102 (= C98), C110 (= C106), R114 (= R110), C173 (= C169), T177 (= T173), R368 (= R370)
binding 12-hydroxydodecanoic acid: T46 (= T42), H66 (= H62), Y92 (= Y88), Q111 (≠ T107), R114 (= R110), C173 (= C169)
binding zinc ion: C44 (= C40), H66 (= H62), C96 (= C92), C99 (= C95), C102 (= C98), C110 (= C106), C173 (= C169)

P11766 Alcohol dehydrogenase class-3; Alcohol dehydrogenase 5; Alcohol dehydrogenase class chi chain; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.1; EC 1.1.1.-; EC 1.1.1.284 from Homo sapiens (Human) (see 3 papers)
58% identity, 99% coverage: 1:373/375 of query aligns to 6:372/374 of P11766

query
sites
P11766

M

I

E

K

S

C

R

K

A

A

V

V

A

A

F

W

E

E

A

A

G

G

K

K

P

P

L

L

E

S

V

I

A

E

T

E

V

I

R

E

L

V

E

A

G

P

P

P

K

K

E

A

G

H

E

E

V

V

L

R

V

I

E

K

I

I

K

I

A

A

T

T

G

A

I

V

C
|
C

H

H

T

T

D

D

E

A

F

Y

T

T

R

L

S

S

G

G

A

A

D

D

P

P

E

E

G

G

I

C

F

F

P

P

A

V

I

I

L

L

G

G

H
|
H

E

E

G

G

A

A

G

G

V

I

V

V

V

E

D

S

V

V

G

G

P

E

G

G

V

V

R

T

S

K

L

L

R

K

K

A

G

G

D

D

H

T

V

V

I

I

P

P

L

L

Y

Y

T

I

P

P

E

Q

C
|
C

R

G

E

E

C
|
C

P

K

S

F

C
|
C

L

L

S

N

R

P

K

K

T

T

N

N

L

L

C
|
C

T

Q

A

K

I

I

R
|
R

T

V

T

T

Q

Q

G

G

Q

K

G

G

L

L

M

M

P

P

D

D

G

G

T

T

S

S

R

R

F

F

S

T

L

C

D

K

G

G

K

K

P

T

I

I

H

L

H

H

Y

Y

M

M

G

G

T

T

S

S

T

T

F

F

S

S

N

E

F

Y

T

T

V

V

L

V

P

A

E

D

I

I

A

S

V

V

A

A

K

K

I

I

R

D

E

P

D
x
L

A

A

P

P

F

L

D

D

K

K

V

V

C

C

Y

L

I

L

G

G

C
|
C

G

G

V

I

T

S

T

T

G

G

I

Y

G

G

A

A

V

A

I

V

N

N

T

T

A

A

K

K

V

L

E

E

A

P

G

G

A

S

T

V

A

C

V

A

V

V

F

F

G

G

L

L

G

G

I

V

G

G

L

L

N

A

V

V

I

I

Q

M

G

G

L

C

R

K

L

V

A

A

G

G

A

A

D

S

M

R

I

I

G

G

V

V

D

D

L

I

N

N

N

K

D

D

K

K

K

F

E

A

W

R

G

A

E

K

R

E

F

F

G

G

M

A

T

T

H

E

F

C

V

I

N

N

P

P

K

Q

E

D

V

F

G

S

S

K

D

P

L

I

V

Q

P

E

H

V

L

L

V

I

N

E

M

M

T

T

K

D

R

-

G

-

A

-

D

-

Q

-

I

-

G

G

A

V

D

D

Y

Y

T

S

F

F

D

E

C

C

T

I

G

G

N

N

V

V

T

K

V

V

M

M

R

R

Q

A

A

A

L

L

E

E

C

A

A

C

H

H

R

K

G

G

W

W

G

G

Q

V

S

S

I

V

V

V

I

V

G

G

V

V

A

A

P

A

A

S

G

G

A

E

E

E

I

I

S

A

T

T

R

R

P

P

F

F

Q

Q

L

L

V
|
V

T

T

G

G

R

R

V

T

W

W

K

K

G

G

T

T

A

A

F

F

G

G

A

W

R

K

G

S

R

V

T

E

D

S

V

V

P

P

K

K

I

L

V

V

E

S

W

E

Y

Y

M

M

E

S

G

K

K

K

I

I

E

K

I

V

D

D

P

E

M

F

I

V

T

T

H

H

T

N

M

L

P

S

L

F

D

D

D

E

I

I

N

N

K

K

G

A

F

F

D

E

L

L

M

M

H

H

H

S

G

G

E

K

S

S

I

I

R

R

S

T

V

V

C45 (= C40) binding Zn(2+)
H67 (= H62) binding Zn(2+)
C97 (= C92) binding Zn(2+)
C100 (= C95) binding Zn(2+)
C103 (= C98) binding Zn(2+)
C111 (= C106) binding Zn(2+)
R115 (= R110) Important for FDH activity and activation by fatty acids; mutation R->A,D: Loss of FDH activity and loss of activation by fatty acids.
L163 (≠ D158) to S: in dbSNP:rs28730623
C174 (= C169) binding Zn(2+)
V309 (= V310) to I: in dbSNP:rs28730628

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

8gv3A The cryo-em structure of gsnor with nyy001
58% identity, 99% coverage: 1:373/375 of query aligns to 4:370/373 of 8gv3A

query
sites
8gv3A

M

I

E

K

S

C

R

K

A

A

V

V

A

A

F

W

E

E

A

A

G

G

K

K

P

P

L

L

E

S

V

I

A

E

T

E

V

I

R

E

L

V

E

A

G

P

P

P

K

K

E

A

G

H

E

E

V

V

L

R

V

I

E

K

I

I

K

I

A

A

T

T

G

A

I

V

C
|
C

H

H

T
|
T

D

D

E

A

F

Y

T

T

R

L

S

S

G

G

A

A

D

D

P

P

E

E

G

G

I

C

F

F

P

P

A

V

I

I

L

L

G

G

H
|
H

E

E

G

G

A

A

G

G

V

I

V

V

V

E

D

S

V

V

G

G

P

E

G

G

V

V

R

T

S

K

L

L

R

K

K

A

G

G

D

D

H

T

V

V

I

I

P

P

L

L

Y
|
Y

T
x
I

P
|
P

E

Q

C

C

R

G

E

E

C

C

P

K

S

F

C
|
C

L

L

S

N

R

P

K

K

T

T

N

N

L
|
L

C
|
C

T

Q

A

K

I

I

R

R

T

V

T

T

Q

Q

G

G

Q

K

G

G

L

L

M

M

P

P

D

D

G

G

T

T

S

S

R

R

F

F

S

T

L

C

D

K

G

G

K

K

P

T

I

I

H

L

H

H

Y

Y

M
|
M

G

G

T

T

S

S

T

T

F

F

S

S

N

E

F

Y

T

T

V

V

L

V

P

A

E

D

I

I

A

S

V

V

A

A

K

K

I

I

R

D

E

P

D

L

A

A

P

P

F

L

D

D

K

K

V

V

C

C

Y

L

I

L

G

G

C
|
C

G

G

V

I

T

S

T

T

G

G

I

Y

G

G

A

A

V

A

I

V

N

N

T

T

A

A

K

K

V

L

E

E

A

P

G

G

A

S

T

V

A

C

V

A

V

V

F

F

G

G

L

L

G

G

I
x
V

G

G

L

L

N

A

V

V

I

I

Q

M

G

G

L

C

R

K

L

V

A

A

G

G

A

A

D

S

M

R

I

I

G

G

V

V

D
|
D

L
x
I

N

N

N

K

D

D

K

K

K

F

E

A

W

R

G

A

E

K

R

E

F

F

G

G

M

A

T

T

H

E

F

C

V

I

N

N

P

P

K

Q

E

D

V

F

G

S

S

K

D

P

L

I

V

Q

P

E

H

V

L

L

V

I

N

E

M

M

T

T

K

D

R

-

G

-

A

-

D

-

Q

-

I

-

G

G

A

V

D

D

Y

Y

T

S

F

F

D

E

C
|
C

T
x
I

G

G

N

N

V

V

T

K

V

V

M

M

R

R

Q

A

A

A

L

L

E

E

C

A

A

C

H

H

R

K

G

G

W

W

G

G

Q

V

S

S

I

V

V

V

I
x
V

G
|
G

V

V

A

A

P

A

A

S

G

G

A

E

E

E

I

I

S

A

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

T

W

W

K

K

G

G

T

T

A

A

F

F

G

G

A

W

R

K

G

S

R

V

T

E

D

S

V

V

P

P

K

K

I

L

V

V

E

S

W

E

Y

Y

M

M

E

S

G

K

K

K

I

I

E

K

I

V

D

D

P

E

M

F

I

V

T

T

H

H

T

N

M

L

P

S

L

F

D

D

D

E

I

I

N

N

K

K

G

A

F

F

D

E

L

L

M

M

H

H

H

S

G

G

E

K

S

S

I

I

R

R

S

T

V

V

binding nicotinamide-adenine-dinucleotide: C172 (= C169), V201 (≠ I198), D221 (= D218), I222 (≠ L219), C266 (= C269), I267 (≠ T270), V290 (≠ I293), G291 (= G294)
binding (4P)-4-{2-[4-(1H-imidazol-1-yl)phenyl]-5-[3-oxo-3-(2-oxo-1,3-thiazolidin-3-yl)propyl]-1H-pyrrol-1-yl}-3-methylbenzamide: T45 (= T42), Y91 (= Y88), I92 (≠ T89), P93 (= P90), L108 (= L105), C109 (= C106), M139 (= M136), C172 (= C169)
binding zinc ion: C43 (= C40), H65 (= H62), C101 (= C98), C109 (= C106), C172 (= C169)

3qj5A S-nitrosoglutathione reductase (gsnor) in complex with n6022 (see paper)
58% identity, 99% coverage: 1:373/375 of query aligns to 4:370/372 of 3qj5A

query
sites
3qj5A

M

I

E

K

S

C

R

K

A

A

V

V

A

A

F

W

E

E

A

A

G

G

K

K

P

P

L

L

E

S

V

I

A

E

T

E

V

I

R

E

L

V

E

A

G

P

P

P

K

K

E

A

G

H

E

E

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V

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R

V

I

E

K

I

I

K

I

A

A

T

T

G

A

I

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C
|
C

H
|
H

T
|
T

D

D

E

A

F
x
Y

T

T

R

L

S

S

G

G

A

A

D

D

P

P

E

E

G

G

I

C

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F

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P

A

V

I

I

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L

G

G

H
|
H

E
|
E

G

G

A

A

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V

I

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E

D

S

V

V

G

G

P

E

G

G

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S

K

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L

R

K

K

A

G

G

D

D

H

T

V

V

I

I

P

P

L

L

Y
|
Y

T
x
I

P

P

E

Q

C
|
C

R

G

E

E

C
|
C

P

K

S

F

C
|
C

L

L

S

N

R

P

K

K

T

T

N

N

L

L

C
|
C

T
x
Q

A

K

I

I

R
|
R

T

V

T

T

Q
|
Q

G

G

Q

K

G

G

L

L

M

M

P

P

D

D

G

G

T

T

S

S

R

R

F

F

S

T

L

C

D

K

G

G

K

K

P

T

I

I

H

L

H

H

Y

Y

M

M

G

G

T

T

S

S

T

T

F

F

S

S

N

E

F

Y

T

T

V

V

L

V

P

A

E

D

I

I

A

S

V

V

A

A

K

K

I

I

R

D

E

P

D

L

A

A

P

P

F

L

D

D

K

K

V

V

C

C

Y

L

I

L

G

G

C
|
C

G

G

V

I

T

S

T
|
T

G

G

I

Y

G

G

A

A

V

A

I

V

N

N

T

T

A

A

K

K

V

L

E

E

A

P

G

G

A

S

T

V

A

C

V

A

V

V

F

F

G

G

L

L

G
|
G

G

G

I
x
V

G

G

L

L

N

A

V

V

I

I

Q

M

G

G

L

C

R

K

L

V

A

A

G

G

A

A

D

S

M

R

I

I

G

G

V

V

D
|
D

L
x
I

N

N

N

K

D

D

K

K

K

F

E

A

W

R

G

A

E

K

R

E

F

F

G

G

M

A

T

T

H

E

F

C

V

I

N

N

P

P

K

Q

E

D

V

F

G

S

S

K

D

P

L

I

V

Q

P

E

H

V

L

L

V

I

N

E

M

M

T

T

K

D

R

-

G

-

A

-

D

-

Q

-

I

-

G

G

A

V

D

D

Y

Y

T

S

F

F

D

E

C
|
C

T
x
I

G

G

N

N

V

V

T

K

V

V

M

M

R

R

Q

A

A

A

L

L

E

E

C

A

A

C

H

H

R

K

G

G

W

W

G

G

Q

V

S

S

I

V

V

V

I
x
V

G
|
G

V
|
V

A

A

P
x
A

A

S

G

G

A

E

E

E

I

I

S

A

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

T

W

W

K

K

G

G

T

T

A
|
A

F
|
F

G

G

A

W

R

K

G

S

R

V

T

E

D

S

V

V

P

P

K

K

I

L

V

V

E

S

W

E

Y

Y

M

M

E

S

G

K

K

K

I

I

E

K

I

V

D

D

P

E

M

F

I

V

T

T

H

H

T

N

M

L

P

S

L

F

D

D

D

E

I

I

N

N

K

K

G

A

F

F

D

E

L

L

M

M

H

H

H

S

G

G

E

K

S

S

I

I

R
|
R

S

T

V

V

active site: C43 (= C40), H44 (= H41), T45 (= T42), Y48 (≠ F45), H65 (= H62), E66 (= E63), C95 (= C92), C98 (= C95), C101 (= C98), C109 (= C106), R113 (= R110), C172 (= C169), T176 (= T173), R367 (= R370)
binding 3-{1-(4-carbamoyl-2-methylphenyl)-5-[4-(1H-imidazol-1-yl)phenyl]-1H-pyrrol-2-yl}propanoic acid: C43 (= C40), T45 (= T42), H65 (= H62), Y91 (= Y88), I92 (≠ T89), Q110 (≠ T107), Q116 (= Q113), C172 (= C169)
binding pentane-1,5-diamine: H44 (= H41), Y48 (≠ F45), V292 (= V295), A294 (≠ P297)
binding nicotinamide-adenine-dinucleotide: C172 (= C169), T176 (= T173), G199 (= G196), V201 (≠ I198), D221 (= D218), I222 (≠ L219), C266 (= C269), I267 (≠ T270), V290 (≠ I293), G291 (= G294), V292 (= V295), A316 (= A319), F317 (= F320)
binding zinc ion: C43 (= C40), H65 (= H62), C95 (= C92), C98 (= C95), C101 (= C98), C109 (= C106), C172 (= C169)

P19854 Alcohol dehydrogenase class-3; Alcohol dehydrogenase 5; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.1; EC 1.1.1.-; EC 1.1.1.284 from Equus caballus (Horse) (see 2 papers)
58% identity, 99% coverage: 1:373/375 of query aligns to 6:372/374 of P19854

query
sites
P19854

M

I

E

K

S

C

R

K

A

A

V

V

A

A

F

W

E

E

A

A

G

G

K

K

P

P

L

V

E

S

V

I

A

E

T

E

V

V

R

E

L

V

E

A

G

P

P

P

K

K

E

A

G

H

E

E

V

V

L

R

V

I

E

K

I

I

K

I

A

A

T

T

G

A

I

V

C

C

H

H

T

T

D

D

E

A

F

Y

T

T

R

L

S

S

G

G

A

A

D

D

P

P

E

E

G

G

I

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F

F

P

P

A

V

I

I

L

L

G

G

H

H

E

E

G

G

A

A

G

G

V

I

V

V

V

E

D

S

V

V

G

G

P

E

G

G

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V

R

T

S

K

L

L

R

K

K

A

G

G

D

D

H

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V

V

I

I

P

P

L

L

Y

Y

T

I

P

P

E

Q

C

C

R

G

E

E

C

C

P

K

S

F

C

C

L

L

S

N

R

P

K

Q

T

T

N

N

L

L

C

C

T

Q

A

K

I

I

R

R

T

T

Q

Q

G

G

Q

K

G

G

L

L

M

M

P

P

D

D

G

G

T

T

S

S

R

R

F

F

S

T

L

C

D

K

G

G

K

K

P

T

I

I

H

L

H

H

Y

Y

M

M

G

G

T

T

S

S

T

T

F

F

S

S

N

E

F

Y

T

T

V

V

L

V

P

A

E

D

I

I

A

S

V

V

A

A

K

K

I

I

R

D

E

P

D

L

A

A

P

P

F

L

D

D

K

K

V

V

C

C

Y

L

I

L

G

G

C

C

G

G

V

V

T

S

T

T

G

G

I

Y

G

G

A

A

V

A

I

V

N

N

T

T

A

A

K

K

V

V

E

E

A

P

G

G

A

S

T

T

A

C

V

A

V

I

F

F

G

G

L

L

G

G

I

V

G

G

L

L

N

A

V

V

I

I

Q

M

G

G

L

C

R

K

L

V

A

A

G

G

A

A

D

S

M

R

I

I

G

G

V

V

D

D

L

I

N

N

N

K

D

D

K

K

K

F

E

A

W

K

G

A

E

K

R

E

F

F

G

G

M

A

T

S

H

E

F

C

V

I

N

N

P

P

K

Q

E

D

V

F

G

S

S

K

D

P

L

I

V

Q

P

E

H

V

L

L

V

I

N

E

M

M

T

T

K

D

R

-

G

-

A

-

D

-

Q

-

I

-

G

G

A

V

D

D

Y

Y

T

S

F

F

D

E

C

C

T

I

G

G

N

N

V

V

T

K

V

V

M

M

R

R

Q

A

A

A

L

L

E

E

C

A

A

C

H

H

R

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G

G

W

W

G

G

Q

V

S

S

I

V

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V

I

V

G

G

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A

A

P

A

A

S

G

G

A

E

E

E

I

I

S

A

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T

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P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

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W

W

K

K

G

G

T

T

A

A

F

F

G

G

A

W

R

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R

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T

E

D

S

V

I

P

P

K

K

I

L

V

V

E

S

W

E

Y

Y

M

M

E

S

G

K

K

K

I

I

E

K

I

V

D

D

P

E

M

F

I

V

T

T

H

H

T

S

M

L

P

S

L

F

D

D

D

Q

I

I

N

N

K

E

G

A

F

F

D

E

L

L

M

M

H

H

H

A

G

G

E

K

S

S

I

I

R

R

S

T

V

V

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylserine

P81431 Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.1; EC 1.1.1.-; EC 1.1.1.284 from Octopus vulgaris (Common octopus) (see paper)
55% identity, 99% coverage: 1:373/375 of query aligns to 8:374/378 of P81431

query
sites
P81431

M

I

E

K

S

C

R

M

A

A

V

I

A

A

F

W

E

E

A

A

G

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K

K

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P

L

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A

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E

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V

R

E

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V

E

A

G

P

P

P

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K

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G

E

E

V

V

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R

V

I

E

K

I

I

K

L

A

H

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S

G

G

I

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C

C

H

H

T

T

D

D

E

A

F

Y

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T

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E

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G

A

I

D

D

P

P

E

E

G

G

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I

F

F

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P

A

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I

I

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L

G

G

H

H

E

E

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G

A

A

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G

V

I

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E

D

S

V

I

G

G

P

E

G

G

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S

L

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R

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K

P

G

G

D

D

H

T

V

V

I

I

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P

L

L

Y

Y

T

V

P

P

E

Q

C

C

R

Y

E

E

C

C

P

K

S

F

C

C

L

L

S

N

R

P

K

K

T

T

N

N

L

L

C

C

T

Q

A

K

I

I

R

R

T

V

T

T

Q

Q

G

G

Q

K

G

G

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M

M

P

P

D

D

G

G

T

T

S

T

R

R

F

F

S

T

L

C

D

K

G

G

K

K

P

E

I

V

H

F

H

H

Y

F

M

M

G

G

T

C

S

S

T

T

F

F

S

S

N

E

F

F

T

T

V

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L

V

P

A

E

E

I

I

A

S

V

I

A

A

K

K

I

V

R

N

E

P

D

K

A

A

P

A

F

L

D

D

K

K

V

V

C

C

Y

L

I

L

G

G

C

C

G

G

V

I

T

S

T

T

G

G

I

Y

G

G

A

A

V

A

I

L

N

N

T

T

A

A

K

A

V

V

E

E

A

K

G

G

A

S

T

N

A

C

V

A

V

V

F

W

G

G

L

L

G

G

G

A

I

V

G

G

L

L

N

A

V

V

I

A

Q

M

G

G

L

C

R

K

L

A

A

A

G

G

A

A

D

A

M

R

I

I

G

G

V

I

D

D

L

I

N

N

N

E

D

S

K

K

K

F

E

E

W

I

G

G

E

K

R

K

F

F

G

G

M

I

T

T

H

E

F

F

V

V

N

N

P

P

K

K

E

K

V

F

G

C

S

K

D

S

L

V

V

Q

P

K

H

V

L

L

V

G

N

E

M

M

T

T

K

D

R

V

G

-

A

-

D

-

Q

-

I

-

G

-

G

G

A

V

D

D

Y

F

T

T

F

F

D

E

C

C

T

I

G

G

N

N

V

V

T

G

V

V

M

M

R

R

Q

A

A

A

L

L

E

E

C

S

A

C

H

H

R

K

G

G

W

W

G

G

Q

T

S

S

I

V

V

I

I

V

G

G

V

V

A

A

P

A

A

H

G

G

A

E

E

E

I

I

S

S

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

V

W

W

K

K

G

G

T

T

A

A

F

F

G

G

A

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K

G

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T

D

D

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V

P

P

K

K

I

L

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V

E

D

W

D

Y

Y

M

M

E

N

G

K

K

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I

L

E

L

I

L

D

D

P

E

M

F

I

V

T

S

H

H

T

K

M

L

P

S

L

F

D

K

I

I

N

N

K

E

G

A

F

F

D

D

L

L

M

M

H

H

H

K

G

G

E

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S

I

I

R

R

S

A

V

V

V

L

Sites not aligning to the query:

1 modified: N-acetylthreonine

8co4C Crystal structure of apo s-nitrosoglutathione reductase from arabidopsis thalina (see paper)
55% identity, 99% coverage: 4:373/375 of query aligns to 11:375/379 of 8co4C

query
sites
8co4C

R

K

A

A

V

V

A

A

F

Y

E

E

A

P

G

N

K

K

P

P

L

L

E

V

V

I

A

E

T

D

V

V

R

Q

L

V

E

A

G

P

P

P

K

Q

E

A

G

G

E

E

V

V

L

R

V

I

E

K

I

I

K

L

A

Y

T

T

G

A

I

L

C
|
C

H

H

T

T

D

D

E

A

F

Y

T

T

R

W

S

S

G

G

A

K

D

D

P

P

E

E

G

G

I

L

F

F

P

P

A

C

I

I

L

L

G

G

H
|
H

E

E

G

A

A

A

G

G

V

I

V

V

V

E

D

S

V

V

G

G

P

E

G

G

V

V

R

T

S

E

L

V

R

Q

K

A

G

G

D

D

H

H

V

V

I

I

P

P

L

C

Y

Y

T

Q

P

A

E

E

C
|
C

R

R

E

E

C
|
C

P

K

S

F

C
|
C

L

K

S

S

R

G

K

K

T

T

N

N

L

L

C
|
C

T

G

A

K

I

V

R

R

T

S

T

A

Q

T

G

G

Q

V

G

G

-

I

L

M

M

M

P

N

D

D

G

R

T

K

S

S

R

R

F

F

S

S

L

V

D

N

G

G

K

K

P

P

I

I

H

Y

H

H

Y

F

M

M

G

G

T

T

S

S

T

T

F

F

S

S

N

Q

F

Y

T

T

V

V

L

V

P

H

E

D

I

V

A

S

V

V

A

A

K

K

I

I

R

D

E

P

D

T

A

A

P

P

F

L

D

D

K

K

V

V

C

C

Y

L

I

L

G

G

C
|
C

G

G

V

V

T

P

T

T

G

G

I

L

G

G

A

A

V

V

I

W

N

N

T

T

A

A

K

K

V

V

E

E

A

P

G

G

A

S

T

N

A

V

V

A

V

I

F

F

G

G

L

L

G

G

G

T

I

V

G

G

L

L

N

A

V

V

I

A

Q

E

G

G

L

A

R

K

L

T

A

A

G

G

A

A

D

S

M

R

I

I

G

G

V

I

D

D

L

I

N

D

N

S

D

K

K

K

K

Y

E

E

W

T

G

A

E

K

R

K

F

F

G

G

M

V

T

N

H

E

F

F

V

V

N

N

P

P

K

K

E

D

V

H

G

D

S

K

D

P

L

I

V

Q

P

E

H

V

L

I

V

V

N

D

M

L

T

T

K

D

R

-

G

-

A

-

D

-

Q

-

I

-

G

G

A

V

D

D

Y

Y

T

S

F

F

D

E

C

C

T

I

G

G

N

N

V

V

T

S

V

V

M

M

R

R

Q

A

A

A

L

L

E

E

C

C

A

C

H

H

R

K

G

G

W

W

G

G

Q

T

S

S

I

V

V

I

I

V

G

G

V

V

A

A

P

A

A

S

G

G

A

Q

E

E

I

I

S

S

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

V

W

W

K

K

G

G

T

T

A

A

F

F

G

G

A

F

R

K

G

S

R

R

T

T

D

Q

V

V

P

P

K

W

I

L

V

V

E

E

W

K

Y

Y

M

M

E

N

G

K

K

E

I

I

E

K

I

V

D

D

P

E

M

Y

I

I

T

T

H

H

T

N

M

L

P

T

L

L

D

G

D

E

I

I

N

N

K

K

G

A

F

F

D

D

L

L

M

L

H

H

H

E

G

G

E

T

S

C

I

L

R

R

S

C

V

V

V

L

binding zinc ion: C47 (= C40), H69 (= H62), C99 (= C92), C102 (= C95), C105 (= C98), C113 (= C106), C177 (= C169)

Q96533 Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; Nitrosoglutathione reductase; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.1; EC 1.1.1.-; EC 1.1.1.-; EC 1.1.1.284 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
55% identity, 99% coverage: 4:373/375 of query aligns to 11:375/379 of Q96533

query
sites
Q96533

R

K

A

A

V

V

A

A

F

Y

E

E

A

P

G

N

K

K

P

P

L

L

E

V

V

I

A

E

T

D

V

V

R

Q

L

V

E

A

G

P

P

P

K

Q

E

A

G

G

E

E

V

V

L

R

V

I

E

K

I

I

K

L

A

Y

T

T

G

A

I

L

C
|
C

H
|
H

T

T

D

D

E

A

F

Y

T

T

R

W

S

S

G

G

A

K

D

D

P

P

E

E

G

G

I

L

F

F

P

P

A

C

I

I

L

L

G

G

H
|
H

E
|
E

G

A

A

A

G

G

V

I

V

V

V

E

D

S

V

V

G

G

P

E

G

G

V

V

R

T

S

E

L

V

R

Q

K

A

G

G

D

D

H

H

V

V

I

I

P

P

L

C

Y

Y

T

Q

P

A

E

E

C
|
C

R

R

E

E

C
|
C

P

K

S

F

C
|
C

L

K

S

S

R

G

K

K

T

T

N

N

L

L

C
|
C

T

G

A

K

I

V

R

R

T

S

T

A

Q

T

G

G

Q

V

G

G

-

I

L

M

M

M

P

N

D

D

G

R

T

K

S

S

R

R

F

F

S

S

L

V

D

N

G

G

K

K

P

P

I

I

H

Y

H

H

Y

F

M

M

G

G

T

T

S

S

T

T

F

F

S

S

N

Q

F

Y

T

T

V

V

L

V

P

H

E

D

I

V

A

S

V

V

A

A

K

K

I

I

R

D

E

P

D

T

A

A

P

P

F

L

D

D

K

K

V

V

C

C

Y

L

I

L

G

G

C
|
C

G

G

V

V

T

P

T

T

G

G

I

L

G

G

A

A

V

V

I

W

N

N

T

T

A

A

K

K

V

V

E

E

A

P

G

G

A

S

T

N

A

V

V

A

V

I

F

F

G
|
G

L
|
L

G
|
G

G
x
T

I
x
V

G
|
G

L

L

N

A

V

V

I

A

Q

E

G

G

L

A

R

K

L

T

A

A

G

G

A

A

D

S

M

R

I

I

G

G

V

I

D
|
D

L

I

N

D

N

S

D

K

K
|
K

K

Y

E

E

W

T

G

A

E

K

R

K

F

F

G

G

M

V

T

N

H

E

F

F

V

V

N

N

P

P

K

K

E

D

V

H

G

D

S

K

D

P

L

I

V

Q

P

E

H

V

L

I

V

V

N

D

M

L

T

T

K

D

R

-

G

-

A

-

D

-

Q

-

I

-

G

G

A

V

D

D

Y

Y

T

S

F

F

D

E

C

C

T
x
I

G

G

N

N

V

V

T

S

V

V

M

M

R

R

Q

A

A

A

L

L

E

E

C

C

A

C

H

H

R

K

G

G

W

W

G

G

Q

T

S

S

I

V

V

I

I
x
V

G
|
G

V
|
V

A

A

P

A

A

S

G

G

A

Q

E

E

I

I

S

S

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

V

W

W

K

K

G

G

T
|
T

A
|
A

F
|
F

G

G

A

F

R

K

G

S

R

R

T

T

D

Q

V

V

P

P

K

W

I

L

V

V

E

E

W

K

Y

Y

M

M

E

N

G

K

K

E

I

I

E

K

I

V

D

D

P

E

M

Y

I

I

T

T

H

H

T

N

M

L

P

T

L

L

D

G

D

E

I

I

N

N

K

K

G

A

F

F

D

D

L

L

M

L

H

H

H

E

G

G

E

T

S

C

I

L

R
|
R

S

C

V

V

V

L

C47 (= C40) binding Zn(2+)
H48 (= H41) binding NAD(+)
H69 (= H62) binding Zn(2+)
E70 (= E63) binding Zn(2+)
C99 (= C92) binding Zn(2+)
C102 (= C95) binding Zn(2+)
C105 (= C98) binding Zn(2+)
C113 (= C106) binding Zn(2+)
C177 (= C169) binding Zn(2+)
GLGTVG 202:207 (≠ GLGGIG 194:199) binding NAD(+)
D226 (= D218) binding NAD(+)
K231 (= K223) binding NAD(+)
I272 (≠ T270) binding NAD(+)
VGV 295:297 (≠ IGV 293:295) binding NAD(+)
TAF 320:322 (= TAF 318:320) binding NAD(+)
R372 (= R370) binding NAD(+)

4gl4A Crystal structure of oxidized s-nitrosoglutathione reductase from arabidopsis thalina, complex with nadh
55% identity, 99% coverage: 1:373/375 of query aligns to 7:374/378 of 4gl4A

query
sites
4gl4A

M

I

E

T

S

C

R

K

A

A

V

V

A

A

F

Y

E

E

A

P

G

N

K

K

P

P

L

L

E

V

V

I

A

E

T

D

V

V

R

Q

L

V

E

A

G

P

P

P

K

Q

E

A

G

G

E

E

V

V

L

R

V

I

E

K

I

I

K

L

A

Y

T

T

G

A

I

L

C
|
C

H
|
H

T
|
T

D

D

E

A

F
x
Y

T

T

R

W

S

S

G

G

A

K

D

D

P

P

E

E

G

G

I

L

F

F

P

P

A

C

I

I

L

L

G

G

H
|
H

E
|
E

G

A

A

A

G

G

V

I

V

V

V

E

D

S

V

V

G

G

P

E

G

G

V

V

R

T

S

E

L

V

R

Q

K

A

G

G

D

D

H

H

V

V

I

I

P

P

L

C

Y
|
Y

T

Q

P

A

E

E

C
|
C

R

R

E

E

C
|
C

P

K

S

F

C
|
C

L

K

S

S

R

G

K

K

T

T

N

N

L

L

C
|
C

T

G

A

K

I

V

R
|
R

T

S

T

A

Q

T

G

G

Q

V

G

G

-

I

L

M

M

M

P

N

D

D

G

R

T

K

S

S

R

R

F

F

S

S

L

V

D

N

G

G

K

K

P

P

I

I

H

Y

H

H

Y

F

M

M

G

G

T

T

S

S

T

T

F

F

S

S

N

Q

F

Y

T

T

V

V

L

V

P

H

E

D

I

V

A

S

V

V

A

A

K

K

I

I

R

D

E

P

D

T

A

A

P

P

F

L

D

D

K

K

V

V

C

C

Y

L

I

L

G

G

C
|
C

G

G

V

V

T

P

T
|
T

G

G

I

L

G

G

A

A

V

V

I

W

N

N

T

T

A

A

K

K

V

V

E

E

A

P

G

G

A

S

T

N

A

V

V

A

V

I

F

F

G

G

L

L

G
|
G

G

T

I
x
V

G

G

L

L

N

A

V

V

I

A

Q

E

G

G

L

A

R

K

L

T

A

A

G

G

A

A

D

S

M

R

I

I

G

G

V

I

D
|
D

L
x
I

N

D

N

S

D

K

K

K

K

Y

E

E

W

T

G

A

E

K

R

K

F

F

G

G

M

V

T

N

H

E

F

F

V

V

N

N

P

P

K

K

E

D

V

H

G

D

S

K

D

P

L

I

V

Q

P

E

H

V

L

I

V

V

N

D

M

L

T

T

K

D

R

-

G

-

A

-

D

-

Q

-

I

-

G

G

A

V

D

D

Y

Y

T

S

F

F

D

E

C
|
C

T
x
I

G

G

N

N

V

V

T

S

V

V

M

M

R

R

Q

A

A

A

L

L

E

E

C

C

A

C

H

H

R

K

G

G

W

W

G

G

Q

T

S

S

I

V

V

I

I
x
V

G
|
G

V
|
V

A

A

P

A

A

S

G

G

A

Q

E

E

I

I

S

S

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

V

W

W

K

K

G

G

T

T

A
|
A

F

F

G

G

A

F

R

K

G

S

R

R

T

T

D

Q

V

V

P

P

K

W

I

L

V

V

E

E

W

K

Y

Y

M

M

E

N

G

K

K

E

I

I

E

K

I

V

D

D

P

E

M

Y

I

I

T

T

H

H

T

N

M

L

P

T

L

L

D

G

D

E

I

I

N

N

K

K

G

A

F

F

D

D

L

L

M

L

H

H

H

E

G

G

E

T

S

C

I

L

R
|
R

S

C

V

V

V

L

active site: C46 (= C40), H47 (= H41), T48 (= T42), Y51 (≠ F45), H68 (= H62), E69 (= E63), C98 (= C92), C101 (= C95), C104 (= C98), C112 (= C106), R116 (= R110), C176 (= C169), T180 (= T173), R371 (= R370)
binding 1,4-dihydronicotinamide adenine dinucleotide: H47 (= H41), Y94 (= Y88), T180 (= T173), G203 (= G196), V205 (≠ I198), D225 (= D218), I226 (≠ L219), C270 (= C269), I271 (≠ T270), V294 (≠ I293), G295 (= G294), V296 (= V295), A320 (= A319)
binding zinc ion: C46 (= C40), H68 (= H62), E69 (= E63), C98 (= C92), C101 (= C95), C104 (= C98), C112 (= C106), C176 (= C169)

3ukoA Crystal structure of s-nitrosoglutathione reductase from arabidopsis thaliana, complex with nadh
55% identity, 99% coverage: 1:373/375 of query aligns to 7:374/378 of 3ukoA

query
sites
3ukoA

M

I

E

T

S

C

R

K

A

A

V

V

A

A

F

Y

E

E

A

P

G

N

K

K

P

P

L

L

E

V

V

I

A

E

T

D

V

V

R

Q

L

V

E

A

G

P

P

P

K

Q

E

A

G

G

E

E

V

V

L

R

V

I

E

K

I

I

K

L

A

Y

T

T

G

A

I

L

C
|
C

H
|
H

T
|
T

D

D

E

A

F
x
Y

T

T

R

W

S

S

G

G

A

K

D

D

P

P

E

E

G

G

I

L

F

F

P

P

A

C

I

I

L

L

G

G

H
|
H

E
|
E

G

A

A

A

G

G

V

I

V

V

V

E

D

S

V

V

G

G

P

E

G

G

V

V

R

T

S

E

L

V

R

Q

K

A

G

G

D

D

H

H

V

V

I

I

P

P

L

C

Y

Y

T

Q

P

A

E

E

C
|
C

R

R

E

E

C
|
C

P

K

S

F

C
|
C

L

K

S

S

R

G

K

K

T

T

N

N

L

L

C
|
C

T

G

A

K

I

V

R
|
R

T

S

T

A

Q

T

G

G

Q

V

G

G

-

I

L

M

M

M

P

N

D

D

G

R

T

K

S

S

R

R

F

F

S

S

L

V

D

N

G

G

K

K

P

P

I

I

H

Y

H

H

Y

F

M

M

G

G

T

T

S

S

T

T

F

F

S

S

N

Q

F

Y

T

T

V

V

L

V

P

H

E

D

I

V

A

S

V

V

A

A

K

K

I

I

R

D

E

P

D

T

A

A

P

P

F

L

D

D

K

K

V

V

C

C

Y

L

I

L

G

G

C
|
C

G

G

V

V

T

P

T
|
T

G

G

I

L

G

G

A

A

V

V

I

W

N

N

T

T

A

A

K

K

V

V

E

E

A

P

G

G

A

S

T

N

A

V

V

A

V

I

F

F

G

G

L

L

G
|
G

G

T

I
x
V

G

G

L

L

N

A

V

V

I

A

Q

E

G

G

L

A

R

K

L

T

A

A

G

G

A

A

D

S

M

R

I

I

G

G

V

I

D
|
D

L
x
I

N

D

N

S

D

K

K
|
K

K

Y

E

E

W

T

G

A

E

K

R

K

F

F

G

G

M

V

T

N

H

E

F

F

V

V

N

N

P

P

K

K

E

D

V

H

G

D

S

K

D

P

L

I

V

Q

P

E

H

V

L

I

V

V

N

D

M

L

T

T

K

D

R

-

G

-

A

-

D

-

Q

-

I

-

G

G

A

V

D

D

Y

Y

T

S

F

F

D

E

C
|
C

T
x
I

G

G

N

N

V

V

T

S

V

V

M

M

R

R

Q

A

A

A

L

L

E

E

C

C

A

C

H

H

R

K

G

G

W

W

G

G

Q

T

S

S

I

V

V

I

I
x
V

G
|
G

V
|
V

A

A

P

A

A

S

G

G

A

Q

E

E

I

I

S

S

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

V

W

W

K

K

G

G

T

T

A
|
A

F

F

G

G

A

F

R

K

G

S

R

R

T

T

D

Q

V

V

P

P

K

W

I

L

V

V

E

E

W

K

Y

Y

M

M

E

N

G

K

K

E

I

I

E

K

I

V

D

D

P

E

M

Y

I

I

T

T

H

H

T

N

M

L

P

T

L

L

D

G

D

E

I

I

N

N

K

K

G

A

F

F

D

D

L

L

M

L

H

H

H

E

G

G

E

T

S

C

I

L

R
|
R

S

C

V

V

V

L

active site: C46 (= C40), H47 (= H41), T48 (= T42), Y51 (≠ F45), H68 (= H62), E69 (= E63), C98 (= C92), C101 (= C95), C104 (= C98), C112 (= C106), R116 (= R110), C176 (= C169), T180 (= T173), R371 (= R370)
binding nicotinamide-adenine-dinucleotide: H47 (= H41), T180 (= T173), G203 (= G196), V205 (≠ I198), D225 (= D218), I226 (≠ L219), K230 (= K223), C270 (= C269), I271 (≠ T270), V294 (≠ I293), G295 (= G294), V296 (= V295), A320 (= A319)
binding zinc ion: C46 (= C40), H68 (= H62), E69 (= E63), C98 (= C92), C101 (= C95), C104 (= C98), C112 (= C106), C176 (= C169)

4dl9A Crystal structure of s-nitrosoglutathione reductase from tomato (solanum lycopersicum) in complex with NAD+ (see paper)
55% identity, 99% coverage: 4:373/375 of query aligns to 11:375/379 of 4dl9A

query
sites
4dl9A

R

K

A

A

V

V

A

A

F

W

E

E

A

P

G

N

K

K

P

P

L

L

E

V

V

I

A

E

T

D

V

V

R

Q

L

V

E

A

G

P

P

P

K

Q

E

A

G

G

E

E

V

V

L

R

V

V

E

K

I

V

K

L

A

Y

T

T

G

A

I

L

C
|
C

H
|
H

T
|
T

D

D

E

A

F
x
Y

T

T

R

W

S

S

G

G

A

K

D

D

P

P

E

E

G

G

I

L

F

F

P

P

A

C

I

V

L

L

G

G

H
|
H

E
|
E

G

A

A

A

G

G

V

I

V

V

V

E

D

S

V

V

G

G

P

E

G

G

V

V

R

T

S

E

L

V

R

Q

K

P

G

G

D

D

H

H

V

V

I

I

P

P

L

C

Y
|
Y

T

Q

P

A

E

E

C
|
C

R

R

E

E

C
|
C

P

K

S

F

C
|
C

L

K

S

S

R

G

K

K

T

T

N

N

L

L

C
|
C

T

G

A

K

I

V

R
|
R

T

A

Q

T

G

G

Q

V

G

G

-

V

L

M

M

M

P

N

D

D

G

R

T

K

S

S

R

R

F

F

S

S

L

I

D

N

G

G

K

K

P

P

I

I

H

Y

H

H

Y

F

M

M

G

G

T

T

S

S

T

T

F

F

S

S

N

Q

F

Y

T

T

V

V

L

V

P

H

E

D

I

V

A

S

V

V

A

A

K

K

I

I

R

D

E

P

D

V

A

A

P

P

F

L

D

E

K

K

V

V

C

C

Y

L

I

L

G

G

C
|
C

G

G

V

V

T

P

T
|
T

G

G

I

L

G

G

A

A

V

V

I

W

N

N

T

T

A

A

K

K

V

V

E

E

A

P

G

G

A

S

T

I

A

V

V

A

V

V

F

F

G

G

L

L

G
|
G

G

T

I
x
V

G

G

L

L

N

A

V

V

I

A

Q

E

G

G

L

A

R

K

L

A

A

A

G

G

A

A

D

S

M

R

I

I

G

G

V

I

D
|
D

L
x
I

N

D

N

S

D

K

K
|
K

K

F

E

D

W

R

G

A

E

K

R

N

F

F

G

G

M

V

T

T

H

E

F

F

V

I

N

N

P

P

K

K

E

E

V

H

G

E

S

Q

D

P

L

I

V

Q

P

Q

H

V

L

I

V

V

N

D

M

L

T

T

K

D

R

-

G

-

A

-

D

-

Q

-

I

-

G

G

A

V

D

D

Y

Y

T

S

F

F

D

E

C
|
C

T
x
I

G

G

N

N

V

V

T

S

V

V

M

M

R

R

Q

S

A

A

L

L

E

E

C

C

A

C

H

H

R

K

G

G

W

W

G

G

Q

T

S

S

I

V

V

I

I
x
V

G
|
G

V
|
V

A

A

P

A

A

S

G

G

A

Q

E

E

I

I

S

S

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

V

W

W

K

K

G

G

T

T

A
|
A

F
|
F

G

G

A

F

R

K

G

S

R

R

T

S

D

Q

V

V

P

P

K

S

I

L

V

V

E

D

W

K

Y

Y

M

L

E

K

G

K

K

E

I

I

E

K

I

V

D

D

P

E

M

Y

I

I

T

T

H

H

T

N

M

M

P

T

L

L

D

A

D

D

I

I

N

N

K

K

G

A

F

F

D

D

L

L

M

M

H

H

H

D

G

G

E

D

S

C

I

L

R
|
R

S

V

V

V

V

L

active site: C47 (= C40), H48 (= H41), T49 (= T42), Y52 (≠ F45), H69 (= H62), E70 (= E63), C99 (= C92), C102 (= C95), C105 (= C98), C113 (= C106), R117 (= R110), C177 (= C169), T181 (= T173), R372 (= R370)
binding nicotinamide-adenine-dinucleotide: Y95 (= Y88), T181 (= T173), G204 (= G196), V206 (≠ I198), D226 (= D218), I227 (≠ L219), K231 (= K223), C271 (= C269), I272 (≠ T270), V295 (≠ I293), G296 (= G294), V297 (= V295), A321 (= A319), F322 (= F320)
binding zinc ion: C47 (= C40), H69 (= H62), E70 (= E63), C99 (= C92), C102 (= C95), C105 (= C98), C113 (= C106), C177 (= C169)

P80572 Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.1; EC 1.1.1.-; EC 1.1.1.284 from Pisum sativum (Garden pea) (Lathyrus oleraceus) (see 2 papers)
54% identity, 99% coverage: 4:373/375 of query aligns to 10:374/378 of P80572

query
sites
P80572

R

K

A

A

V

V

A

A

F

W

E

E

A

P

G

N

K

K

P

P

L

L

E

T

V

I

A

E

T

D

V

V

R

E

L

V

E

A

G

P

P

P

K

Q

E

A

G

N

E

E

V

V

L

R

V

I

E

Q

I

I

K

L

A

F

T

T

G

A

I

L

C

C

H

H

T

T

D

D

E

A

F

Y

T

T

R

L

S

G

G

G

A

K

D

D

P

P

E

E

G

G

I

L

F

F

P

P

A

C

I

I

L

L

G

G

H

H

E

E

G

A

A

A

G

G

V

I

V

V

V

E

D

S

V

V

G

G

P

E

G

G

V

V

R

T

S

D

L

V

R

K

K

P

G

G

D

D

H

H

V

V

I

I

P

P

L

S

Y

Y

T

Q

P

A

E

E

C

C

R

G

E

E

C

C

P

K

S

F

C

C

L

K

S

S

R

P

K

K

T

T

N

N

L

L

C

C

T

G

A

K

I

V

R

R

T

A

Q

T

G

G

Q

V

G

G

-

V

L

M

M

M

P

A

D

D

G

R

T

K

S

S

R

R

F

F

S

S

L

V

D

K

G

G

K

K

P

P

I

I

H

Y

H

H

Y

F

M

M

G

G

T

T

S

S

T

T

F

F

S

S

N

Q

F

Y

T

T

V

V

L

V

P

H

E

D

I

V

A

S

V

V

A

A

K

K

I

I

R

H

E

P

D

D

A

A

P

P

F

L

D

D

K

K

V

V

C

C

Y

L

I

L

G

G

C

C

G

G

V

V

T

P

T

T

G

G

I

L

G

G

A

A

V

V

I

W

N

N

T

T

A

A

K

K

V

V

E

E

A

P

G

G

A

S

T

I

A

V

V

A

V

I

F

F

G

G

L

L

G

G

G

T

I

V

G

G

L

L

N

A

V

V

I

A

Q

E

G

G

L

A

R

K

L

S

A

A

G

G

A

A

D

S

M

R

I

I

G

G

V

I

D

D

L

I

N

D

N

S

D

N

K

K

K

Y

E

D

W

T

G

A

E

K

R

N

F

F

G

G

M

V

T

T

H

E

F

F

V

I

N

N

P

P

K

K

E

D

V

H

G

E

S

K

D

P

L

I

V

Q

P

Q

H

V

L

I

V

I

N

D

M

L

T

T

K

D

R

-

G

-

A

-

D

-

Q

-

I

-

G

G

A

V

D

D

Y

Y

T

S

F

F

D

E

C

C

T

L

G

G

N

N

V

V

T

S

V

V

M

M

R

R

Q

S

A

A

L

L

E

E

C

C

A

C

H

H

R

K

G

G

W

W

G

G

Q

T

S

S

I

V

V

I

I

V

G

G

V

V

A

A

P

A

A

S

G

G

A

Q

E

E

I

I

S

S

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

V

W

W

K

K

G

G

T

T

A

A

F

F

G

G

A

F

R

K

G

S

R

R

T

S

D

Q

V

V

P

P

K

W

I

L

V

V

E

E

W

K

Y

Y

M

L

E

K

G

K

K

E

I

I

E

K

I

V

D

D

P

E

M

Y

I

I

T

T

H

H

T

N

M

L

P

T

L

L

D

L

D

E

I

I

N

N

K

K

G

A

F

F

D

D

L

L

M

L

H

H

H

E

G

G

E

Q

S

C

I

L

R

R

S

C

V

V

V

L

Sites not aligning to the query:

1 modified: N-acetylalanine

7aauC Crystal structure of nitrosoglutathione reductase from chlamydomonas reinhardtii in complex with NAD+ (see paper)
55% identity, 100% coverage: 1:375/375 of query aligns to 9:378/378 of 7aauC

query
sites
7aauC

M

I

E

E

S

C

R

K

A

A

V

I

A

A

F

W

E

E

A

A

G

K

K

K

P

P

L

L

E

E

V

V

A

R

T

T

V

V

R

T

L

V

E

A

G

P

P

P

K

G

E

P

G

G

E

E

V

V

L

R

V

V

E

Q

I

I

K

K

A

A

T

T

G

A

I

L

C
|
C

H

Q

T
|
T

D

D

E

A

F
x
Y

T

T

R

L

S

G

G

G

A

L

D

D

P

P

E

E

G

G

I

R

F

F

P

P

A

C

I

I

L

L

G

G

H
|
H

E

E

G

A

A

A

G

G

V

V

V

E

D

S

V

V

G

G

P

E

G

G

V

V

R

T

S

S

L

V

R

K

K

P

G

G

D

D

H

H

V

V

I

I

P

P

L

C

Y

Y

T

Q

P

A

E

Y

C
|
C

R

G

E

E

C
|
C

P

K

S

F

C
|
C

L

K

S

H

R

P

K

E

T

S

N

N

L

L

C
|
C

T

V

A

S

I

V

R

R

T

A

T

F

Q

T

G

G

Q

K

G

G

L

V

M

M

-

K

P

S

D

D

G

G

T

K

S

P

R

R

F

F

S

T

L

V

D

D

G

G

K

K

P

P

I

I

H

Y

H

H

Y

F

M

M

G

G

T

T

S

S

T

T

F

F

S

S

N

E

F

Y

T

T

V

V

L

V

P

H

E

E

I

Q

A

S

V

V

A

A

K

K

I

I

R

D

E

V

D

N

A

A

P

P

F

L

D

D

K

K

V

V

C

C

Y

L

I

L

G

G

C
|
C

G

G

V

V

T

S

T
|
T

G

G

I

W

G

G

A

A

V

V

I

F

N

N

T

T

A

A

K

K

V

V

E

T

A

A

G

G

A

S

T

T

A

V

V

A

V

V

F

F

G
|
G

L

L

G
|
G

G

A

I
x
V

G

G

L

L

N

A

V

V

I

I

Q

E

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K

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G

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A

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V

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D
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I

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K

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F

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G

A

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F

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H

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-

I

-

G

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D

-

Y

Y

T

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F

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D

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C
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C

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I

G

G

N

N

V

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T

A

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R

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V

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F

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L

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N

M

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H

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G

G

E

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I

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R
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R

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C

V

V

V

L

L

T

F

F

active site: C48 (= C40), T50 (= T42), Y53 (≠ F45), H70 (= H62), C178 (= C169)
binding nicotinamide-adenine-dinucleotide: T182 (= T173), G203 (= G194), G205 (= G196), V207 (≠ I198), D227 (= D218), I228 (≠ L219), C272 (= C269), I273 (≠ T270), V296 (≠ I293), G297 (= G294), V298 (= V295), A322 (= A319), F323 (= F320), R373 (= R370)
binding zinc ion: C48 (= C40), H70 (= H62), C100 (= C92), C103 (= C95), C106 (= C98), C114 (= C106), C178 (= C169)

Query Sequence

>WP_023430621.1 NCBI__GCF_000496075.1:WP_023430621.1
MESRAAVAFEAGKPLEVATVRLEGPKEGEVLVEIKATGICHTDEFTRSGADPEGIFPAIL
GHEGAGVVVDVGPGVRSLRKGDHVIPLYTPECRECPSCLSRKTNLCTAIRTTQGQGLMPD
GTSRFSLDGKPIHHYMGTSTFSNFTVLPEIAVAKIREDAPFDKVCYIGCGVTTGIGAVIN
TAKVEAGATAVVFGLGGIGLNVIQGLRLAGADMIIGVDLNNDKKEWGERFGMTHFVNPKE
VGSDLVPHLVNMTKRGADQIGGADYTFDCTGNVTVMRQALECAHRGWGQSIVIGVAPAGA
EISTRPFQLVTGRVWKGTAFGGARGRTDVPKIVEWYMEGKIEIDPMITHTMPLDDINKGF
DLMHHGESIRSVVLF

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory