SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_023431505.1 NCBI__GCF_000496075.1:WP_023431505.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 1 hits to proteins with known functional sites (download)

P0AAC6 Modulator of FtsH protease YccA from Escherichia coli (strain K12) (see 2 papers)
30% identity, 57% coverage: 103:242/245 of query aligns to 80:214/219 of P0AAC6

query
sites
P0AAC6

W

F

A

A

F

F

A

T

A

G

L

F

M

L

G

G

L

Y

S

I

L

L

S

G

S

P

I

I

F

L

L

N

V

T

Y

Y

-

L

-

S

-

A

-

G

T

M

G

G

N

D

S

V

I

I

A

A

R

M

V

A

F

L

F

G

I

G

T

T

A

A

A

L

S

V

F

F

G

F

A

C

L

C

S

S

L

A

Y

Y

G

V

Y

L

T

T

T

T

A

R

K

K

D

D

L

M

S

S

G

F

W

L

G

G

S

G

F

M

L

L

F

M

M

A

G

G

L

I

I

V

G

V

I

V

I

L

I

I

A

G

S

M

I

V

V

A

N

N

L

I

F

F

I

L

G

Q

S

L

S

P

A

A

V

L

Q

H

F

L

A

A

V

I

S

S

V

A

I

V

G

F

V

I

L

L

V

I

F

S

A

S

G

G

L

A

T

I

A

L

Y

F

D

E

T

T

Q

S

Q

N

I

I

K

-

E

-

M

-

Y

I

Y

H

E

G

G

G

D

E

S

T

A

-

L

-

V

-

S

-

S

-

R

-

K

N

A

Y

I

I

M

R

G

A

A

T

L

V

R

S

L

L

Y

Y

L

V

D

S

F

L

I

Y

N

N

L

I

F

F

V

V

M

S

L

L

L

L

S

S

L

I

F

L

G

G

Sites not aligning to the query:

1:12 MDRIVSSSHDRT→MTMITPSLHDRI: Protein is as sensitive to FtsH degradation as the wild-type.
5:12 mutation Missing: In yccA11; increases the half-life of overexpressed SecY, F(0) ATP synthase subunit a and itself in an HflKC-dependent fashion, but has no effect on degradation of cytosolic substrates sigma-32 or the phage lambda cII protein. Partially dominant over the wild-type allele. This protein interacts with but is not degraded by FtsH.

Query Sequence

>WP_023431505.1 NCBI__GCF_000496075.1:WP_023431505.1
MAEWNRQAAPRYGTATGARVGEIDQGLRSYMLQVYNYMAIGLAVTGVVAFGAFTMAVNSP
DFAQLMYGSPLRWVIIFAPLAMVFFLSARMHKMSTSAAQMAFWAFAALMGLSLSSIFLVY
TGNSIARVFFITAASFGALSLYGYTTAKDLSGWGSFLFMGLIGIIIASIVNLFIGSSAVQ
FAVSVIGVLVFAGLTAYDTQQIKEMYYEGDSALVSSRKAIMGALRLYLDFINLFVMLLSL
FGNRE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory