SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_023432784.1 NCBI__GCF_000496075.1:WP_023432784.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q9HTJ1 NAD/NADP-dependent betaine aldehyde dehydrogenase; BADH; EC 1.2.1.8 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 2 papers)
54% identity, 100% coverage: 2:488/488 of query aligns to 1:490/490 of Q9HTJ1

query
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Q9HTJ1

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GAWN 150:153 (= GAWN 150:153) binding NADPH
K162 (= K162) active site, Charge relay system
KPSE 176:179 (= KPSE 176:179) binding NADPH
G209 (= G208) binding NADPH
GTST 230:233 (≠ SVPT 229:232) binding NADPH
E252 (= E250) active site, Proton acceptor
C286 (= C284) binding covalent; modified: Cysteine sulfenic acid (-SOH)
E387 (= E385) binding NADPH
E464 (= E462) active site, Charge relay system

4cazA Crystal structure of betaine aldehyde dehydrogenase from pseudomonas aeruginosa in complex with nadh
54% identity, 99% coverage: 7:488/488 of query aligns to 5:489/489 of 4cazA

query
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4cazA

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active site: N152 (= N153), K175 (= K176), E251 (= E250), C285 (= C284), E386 (= E385), E463 (= E462)
binding [[(2R,3S,4R,5R)-5-[(3R)-3-aminocarbonyl-3,4-dihydro-2H-pyridin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanidyl-phosphoryl] [(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl phosphate: I148 (= I149), G149 (= G150), W151 (= W152), N152 (= N153), K175 (= K176), E178 (= E179), G208 (= G208), G212 (= G212), F226 (≠ V226), T227 (= T227), G228 (= G228), G229 (≠ S229), T232 (= T232), V236 (= V236), E251 (= E250), L252 (= L251), C285 (= C284), E386 (= E385), F388 (= F387)

2woxA Betaine aldehyde dehydrogenase from pseudomonas aeruginosa with NAD(p) h-catalytic thiol adduct. (see paper)
54% identity, 99% coverage: 7:488/488 of query aligns to 5:489/489 of 2woxA

query
sites
2woxA

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V

M

M

A

A

S

S

A

A

A

S

G

S

T

S

-

S

L

L

K

K

H

E

V

V

T

T

M

M

E
|
E

L
|
L

G

G

G

G

K

K

S

S

A

P

L

L

L

I

V

I

F

F

A

P

D

D

A

A

D

D

L

L

D

D

D

R

A

A

V

A

S

D

A

I

A

A

M

V

L

M

A

A

N

N

F

F

Y

F

T

S

Q

S

G

G

E

Q

I

V

C
|
C

T

T

N

N

G

G

T

T

R

R

V

V

F

F

V

I

E

H

R

R

P

S

V

Q

C

Q

E

A

A

R

F

F

L

E

E

A

K

K

A

V

K

L

A

E

R

R

A

V

E

Q

A

R

I

I

R

R

I

L

G

G

D

D

P

P

L

Q

D

D

P

E

E

N

T

T

Q

N

M

F

G

G

P

P

L

L

I

V

H

S

A

F

Q

P

H

H

L

M

D

E

K

S

V

V

M

L

K

G

F

Y

V

I

E

E

N

S

G

G

V

K

R

A

E

Q

G

K

A

A

R

R

L

L

V

L

T

C

G

G

G

G

K

E

R

R

L

V

T

T

D

D

G

G

D

A

F

F

A

G

K

K

G

G

N

A

F

Y

M

V

Q

A

P

P

T

T

I

V

F

F

A

T

D

D

V

C

L

R

D

D

Q

D

M

M

T

T

I

I

C

V

M

R

Q

E

E
|
E

I

I

F
|
F

G

G

P

P

V

V

M

M

S

S

V

I

L

L

P

V

F

Y

E

D

S

D

E

E

E

D

E

E

A

A

L

I

R

R

R

R

A

A

N

N

D

D

T

T

P

E

F

Y

G

G

L

L

A

A

A

A

G

G

V

V

M

V

T

T

R

Q

D

D

I

L

S

A

R

R

A

A

H

H

R

R

V

A

A

I

A

H

K

R

L

L

E

E

A

A

G

G

V

I

V

C

W

W

V

I

N

N

H

T

Y

W

N

G

L

E

T

S

P

P

I

A

P

E

M

M

P

P

F

V

G

G

G

G

S

Y

K

K

M

Q

S

S

G

G

L

V

G

G

R

R

E
|
E

N

N

G

G

L

L

A

T

A

T

L

L

D

A

F

H

Y

Y

T

T

E

R

R

I

K

K

S

S

V

V

Y

Q

V

V

N

E

L

L

G

G

G

D

V

Y

E

A

S

S

P

V

Y

F

active site: N152 (= N153), K175 (= K176), E251 (= E250), C285 (= C284), E386 (= E385), E463 (= E462)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: I148 (= I149), G149 (= G150), W151 (= W152), N152 (= N153), K175 (= K176), S177 (= S178), E178 (= E179), G208 (= G208), G212 (= G212), F226 (≠ V226), T227 (= T227), G228 (= G228), G229 (≠ S229), T232 (= T232), V236 (= V236), E251 (= E250), L252 (= L251), C285 (= C284), E386 (= E385), F388 (= F387)

2wmeA Crystallographic structure of betaine aldehyde dehydrogenase from pseudomonas aeruginosa (see paper)
54% identity, 99% coverage: 7:488/488 of query aligns to 5:489/489 of 2wmeA

query
sites
2wmeA

E

E

I

Q

S

K

C

L

H

Y

I

I

A

G

G

G

R

R

R

Y

I

V

P

E

G

A

S

S

-

S

G

G

D

A

A

T

L

F

S

E

S

T

I

I

N

N

P

P

A

A

N

N

G

G

E

E

T

V

L

L

A

A

T

K

G

V

N

Q

A

R

A

A

A

S

M

R

P

E

E

D

I

V

E

E

Q

R

A

A

I

V

E

Q

A

S

A

A

R

V

E

E

A

G

R

Q

R

K

S

V

W

W

M

A

A

A

M

M

S

T

A

A

A

M

E

Q

R

R

G

S

R

R

V

I

L

L

A

R

R

R

V

A

A

V

A

D

I

I

L

L

R

R

E

E

R

R

R

N

D

D

D

E

L

L

A

A

R

A

L

L

E

E

V

T

L

L

D

D

T

T

G

G

K

K

P

P

I

L

Q

A

E

E

A

T

P

R

A

S

A

V

D

D

I

I

D

V

S

T

A

G

A

A

D

D

C

V

F

L

E

E

F

Y

F

Y

A

A

G

G

L

L

T

V

Q

P

I

A

V

I

Q

E

G

G

E

E

H

Q

I

I

P

P

F

L

G

R

D

E

D

T

G

S

F

F

A

V

Y

Y

T

T

R

R

R

R

E

E

P

P

L

L

G

G

I

V

C

V

A

A

G

G

I

I

G
|
G

A

A

W
|
W

N
|
N

Y

Y

P

P

I

V

Q

Q

I

I

A

A

S

L

W

W

K

K

T

S

A

A

P

P

A

A

L

L

A

A

A

A

G

G

N

N

A

A

M

M

L

I

F

F

K
|
K

P

P

S
|
S

E
|
E

V

V

T

T

P

P

L

L

T

T

A

A

L

L

E

K

L

L

G

A

R

E

I

I

V

Y

E

T

E

E

A

A

G

G

A

V

P

P

P

D

G

G

L

V

L

F

S

N

I

V

L

L

P

T

G

G

-

S

G
|
G

R

R

E

E

T

V

G
|
G

E

Q

I

W

L

L

S

T

T

E

H

H

P

P

A

L

I

I

A

E

K

K

I

I

S

S

V
x
F

T

T

G
|
G

S
x
G

V

T

P

S

T
|
T

G

G

K

K

A

K

V
|
V

M

M

A

A

S

S

A

A

A

S

G

S

T

S

-

S

L

L

K

K

H

E

V

V

T

T

M

M

E
|
E

L

L

G

G

G

G

K

K

S

S

A

P

L

L

L

I

V

I

F

F

A

P

D

D

A

A

D

D

L

L

D

D

D

R

A

A

V

A

S

D

A

I

A

A

M

V

L

M

A

A

N

N

F

F

Y

F

T

S

Q

S

G

G

E

Q

I

V

C
|
C

T

T

N

N

G

G

T

T

R

R

V

V

F

F

V

I

E

H

R

R

P

S

V

Q

C

Q

E

A

A

R

F

F

L

E

E

A

K

K

A

V

K

L

A

E

R

R

A

V

E

Q

A

R

I

I

R

R

I

L

G

G

D

D

P

P

L

Q

D

D

P

E

E

N

T

T

Q

N

M

F

G

G

P

P

L

L

I

V

H

S

A

F

Q

P

H

H

L

M

D

E

K

S

V

V

M

L

K

G

F

Y

V

I

E

E

N

S

G

G

V

K

R

A

E

Q

G

K

A

A

R

R

L

L

V

L

T

C

G

G

G

G

K

E

R

R

L

V

T

T

D

D

G

G

D

A

F

F

A

G

K

K

G

G

N

A

F

Y

M

V

Q

A

P

P

T

T

I

V

F

F

A

T

D

D

V

C

L

R

D

D

Q

D

M

M

T

T

I

I

C

V

M

R

Q

E

E
|
E

I

I

F

F

G

G

P

P

V

V

M

M

S

S

V

I

L

L

P

V

F

Y

E

D

S

D

E

E

E

D

E

E

A

A

L

I

R

R

R

R

A

A

N

N

D

D

T

T

P

E

F

Y

G

G

L

L

A

A

A

A

G

G

V

V

M

V

T

T

R

Q

D

D

I

L

S

A

R

R

A

A

H

H

R

R

V

A

A

I

A

H

K

R

L

L

E

E

A

A

G

G

V

I

V

C

W

W

V

I

N

N

H

T

Y

W

N

G

L

E

T

S

P

P

I

A

P

E

M

M

P

P

F

V

G

G

G

G

S

Y

K

K

M

Q

S

S

G

G

L

V

G

G

R

R

E
|
E

N

N

G

G

L

L

A

T

A

T

L

L

D

A

F

H

Y

Y

T

T

E

R

R

I

K

K

S

S

V

V

Y

Q

V

V

N

E

L

L

G

G

G

D

V

Y

E

A

S

S

P

V

Y

F

active site: N152 (= N153), K175 (= K176), E251 (= E250), C285 (= C284), E386 (= E385), E463 (= E462)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G149 (= G150), W151 (= W152), K175 (= K176), S177 (= S178), E178 (= E179), G208 (= G208), G212 (= G212), F226 (≠ V226), G228 (= G228), G229 (≠ S229), T232 (= T232), V236 (= V236)

1bpwA Betaine aldehyde dehydrogenase from cod liver (see paper)
53% identity, 98% coverage: 11:488/488 of query aligns to 23:503/503 of 1bpwA

query
sites
1bpwA

H

Y

I

W

A

G

G

G

R

R

R

R

I

I

P

K

G

S

S

K

G

D

D

G

A

A

L

T

S

T

S

E

-

P

-

V

I

F

N

E

P

P

A

A

N

T

G

G

E

R

T

V

L

L

A

C

T

Q

G

M

N

V

A

P

A

C

A

G

M

A

P

E

E

E

I

V

E

D

Q

Q

A

A

I

V

E

Q

A

S

A

A

R

Q

E

A

A

A

R

Y

R

L

S

K

W

W

M

S

A

K

M

M

S

A

A

G

A

I

E

E

R

R

G

S

R

R

V

V

L

M

A

L

R

E

V

A

A

A

A

R

I

I

L

I

R

R

E

E

R

R

R

R

D

D

D

N

L

I

A

A

R

K

L

L

E

E

V

V

L

I

D

N

T

N

G

G

K

K

P

T

I

I

Q

T

E

E

A

A

P

-

A

E

A

Y

D

D

I

I

D

D

S

A

A

A

A

W

D

Q

C

C

F

I

E

E

F

Y

F

Y

A

A

G

G

L

L

T

A

Q

P

I

T

V

L

Q

S

G

G

E

Q

H

H

I

I

P

Q

F

L

G

P

D

G

D

G

G

A

F

F

A

A

Y

Y

T

T

R

R

R

R

E

E

P

P

L

L

G

G

I

V

C

C

A

A

G

G

I
|
I

G
x
L

A

A

W
|
W

N
|
N

Y

Y

P

P

I

F

Q

M

I

I

A

A

S

A

W

W

K

K

T

C

A

A

P

P

A

A

L

L

A

A

A

C

G

G

N

N

A

A

M

V

L

V

F

F

K
|
K

P

P

S

S

E

P

V

M

T

T

P

P

L

V

T

T

A

G

L

V

E

I

L

L

G

A

R

E

I

I

V

F

E

H

E

E

A

A

G

G

A

V

P

P

P

V

G

G

L

L

L

V

S

N

I

V

L

V

P

Q

G

G

G
|
G

R

A

E

E

T

T

G
|
G

E

S

I

L

L

L

S

C

T

H

H

H

P

P

A

N

I

V

A

A

K

K

I

V

S

S

V

F

T
|
T

G
|
G

S
|
S

V

V

P

P

T
|
T

G

G

K

K

A

K

V

V

M

M

A

E

S

M

A

S

A

A

G

K

T

T

L

V

K

K

H

H

V

V

T

T

M

L

E
|
E

L
|
L

G

G

G

G

K

K

S

S

A

P

L

L

L

L

V

I

F

F

A

K

D

D

A

C

D

E

L

L

D

E

D

N

A

A

V

V

S

R

A

G

A

A

M

L

L

M

A

A

N

N

F

F

Y

L

T

T

Q

Q

G

G

E

Q

I

V

C
|
C

T

T

N

N

G

G

T

T

R

R

V

V

F

F

V

V

E

Q

R

R

P

E

V

I

C

M

E

P

A

Q

F

F

L

L

E

E

K

E

A

V

K

V

A

K

R

R

A

T

E

K

A

A

I

I

R

V

I

V

G

G

D

D

P

P

L

L

D

L

P

T

E

E

T

T

Q

R

M

M

G

G

P

G

L

L

I

I

H

S

A

K

Q

P

H

Q

L

L

D

D

K

K

V

V

M

L

K

G

F

F

V

V

E

A

N

Q

G

A

V

K

R

K

E

E

G

G

A

A

R

R

L

V

V

L

T

C

G

G

G

G

K

E

R

P

L

L

T

T

D

P

G

S

D

D

-

P

-

K

F

L

A

K

K

N

G

G

N

Y

F

F

M

M

Q

S

P

P

T

C

I

V

F

L

A

D

D

N

V

C

L

R

D

D

Q

D

M

M

T

T

I

C

C

V

M

K

Q

E

E
|
E

I

I

F
|
F

G

G

P

P

V

V

M

M

S

S

V

V

L

L

P

P

F

F

E

D

S

T

E

E

E

E

E

E

A

V

L

L

R

Q

R

R

A

A

N

N

D

N

T

T

P

T

F

F

G

G

L

L

A

A

A

S

G

G

V

V

M

F

T

T

R

R

D

D

I

I

S

S

R

R

A

A

H

H

R

R

V

V

A

A

A

A

K

N

L

L

E

E

A

A

G

G

V

T

V

C

W

Y

V

I

N

N

H

T

Y

Y

N

S

L

I

T

S

P

P

I

V

P

E

M

V

P

P

F
|
F

G

G

G

G

S

Y

K

K

M

M

S

S

G

G

L

F

G

G

R

R

E
|
E

N

N

G

G

L

Q

A

A

A

T

L

V

D

D

F

Y

Y

Y

T

S

E

Q

R

L

K

K

S

T

V

V

Y

I

V

V

N

E

L

M

G

G

G

D

V

V

E

D

S

S

P

L

Y

F

active site: N166 (= N153), K189 (= K176), E263 (= E250), C297 (= C284), E400 (= E385), E477 (= E462)
binding nicotinamide-adenine-dinucleotide: I162 (= I149), L163 (≠ G150), W165 (= W152), N166 (= N153), K189 (= K176), G221 (= G208), G225 (= G212), T240 (= T227), G241 (= G228), S242 (= S229), T245 (= T232), E263 (= E250), L264 (= L251), C297 (= C284), E400 (= E385), F402 (= F387), F466 (= F451)

P56533 4-trimethylaminobutyraldehyde dehydrogenase; TMABA-DH; TMABADH; Aldehyde dehydrogenase family 9 member A1; Betaine aldehyde dehydrogenase; BADH; EC 1.2.1.47; EC 1.2.1.3 from Gadus morhua subsp. callarias (Baltic cod) (Gadus callarias) (see paper)
53% identity, 98% coverage: 11:488/488 of query aligns to 23:503/503 of P56533

query
sites
P56533

H

Y

I

W

A

G

G

G

R

R

R

R

I

I

P

K

G

S

S

K

G

D

D

G

A

A

L

T

S

T

S

E

-

P

-

V

I

F

N

E

P

P

A

A

N

T

G

G

E

R

T

V

L

L

A

C

T

Q

G

M

N

V

A

P

A

C

A

G

M

A

P

E

E

E

I

V

E

D

Q

Q

A

A

I

V

E

Q

A

S

A

A

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Q

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A

A

A

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Y

R

L

S

K

W

W

M

S

A

K

M

M

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A

A

G

A

I

E

E

R

R

G

S

R

R

V

V

L

M

A

L

R

E

V

A

A

A

A

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I

I

L

I

R

R

E

E

R

R

R

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D

D

D

N

L

I

A

A

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L

L

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V

V

L

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N

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N

G

G

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K

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-

A

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A

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D

D

I

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A

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C

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F

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F

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H

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G

G

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C

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A

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W

N

N

Y

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P

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M

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W

K

K

T

C

A

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P

A

A

L

L

A

A

A

C

G

G

N

N

A

A

M

V

L

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F

F

K
|
K

P

P

S

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M

T

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A

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H

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K

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G
|
G

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|
S

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|
V

P
|
P

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|
T

G

G

K

K

A

K

V

V

M

M

A

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M

A

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L

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K

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H

H

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L

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D

D

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L

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N

A

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N

F

F

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N

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K

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M

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L

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I

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D

D

K

K

V

V

M

L

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F

F

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V

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G

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P

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|
E

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R

D

D

I

I

S

S

R

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A

A

H

H

R

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V

V

A

A

A

A

K

N

L

L

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E

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A

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G

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W

Y

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N

N

H

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Y

Y

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L

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M

V

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P

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F

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G

G

G

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Y

K

K

M

M

S

S

G

G

L

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G

G

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R

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N

N

G

G

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A

A

A

T

L

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D

D

F

Y

Y

Y

T

S

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K

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T

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N

E

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G

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S

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L

Y

F

K189 (= K176) binding NAD(+)
GSVPT 241:245 (= GSVPT 228:232) binding NAD(+)
E400 (= E385) binding NAD(+)

8skfA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (lattice translocation disorder)
53% identity, 100% coverage: 2:488/488 of query aligns to 8:497/497 of 8skfA

query
sites
8skfA

M

M

S

S

T

R

V

M

L

A

E

E

I

Q

S

Q

C

L

H

Y

I

I

A

H

G

G

R

K

R

F

I

V

P

A

G

A

-

T

S

S

G

G

D

K

A

T

L

F

S

E

S
x
T

I
|
I

N

N

P

P

A

A

N

T

G

G

E

E

T

V

L

L

A

A

T

T

G

V

N

Q

A

A

A

A

A

G

M

R

P

E

E

D

I

V

E

D

Q

R

A

A

I

V

E

K

A

S

A

A

R

Q

E

Q

A

G

R

Q

R

K

S

V

W

W

M

A

A

A

M

M

S

S

A

A

A

M

E

A

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R

G

S

R

R

V

I

L

L

A

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K

V

A

A

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A

D

I

I

L

L

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R

E

E

R

R

R

N

D

D

D

E

L

L

A

A

R

R

L

L

E

E

V

T

L

L

D
|
D

T

T

G

G

K

K

P

P

I

L

Q

S

E

E

A

T

P

A

A

A

A

V

D

D

I

I

D

V

S

T

A

G

A

A

D

D

C

V

F

L

E

E

F

Y

F

Y

A

A

G

G

L

L

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I

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P

I

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V

L

Q

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G

G

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H

Q

I

I

P

P

F

L

G

R

D

D

D

S

G

S

F

F

A

V

Y

Y

T

T

R

R

R

R

E

E

P

P

L

L

G

G

I

V

C

V

A

A

G

G

I
|
I

G
|
G

A
|
A

W
|
W

N

N

Y

Y

P

P

I

I

Q

Q

I

I

A

A

S

L

W

W

K

K

T

S

A

A

P

P

A

A

L

L

A

A

A

A

G

G

N

N

A

A

M

M

L

I

F

F

K
|
K

P

P

S

S

E
|
E

V
|
V

T

T

P

P

L

L

T

T

A

A

L

L

E

K

L

L

G

A

R

E

I

I

V

Y

E

R

E

E

A

A

G

G

A

L

P

P

P

D

G

G

L

V

L

F

S

N

I

V

L

L

P

P

G

G

-

I

G
|
G

R

A

E

E

T

T

G
|
G

E

Q

I

Y

L

L

S

T

T

E

H

H

P

P

A

D

I

I

A

A

K

K

I

I

S

S

V

F

T
|
T

G
|
G

S
x
G

V

V

P

A

T
x
S

G

G

K

K

A
x
K

V

V

M

M

A

A

-

N

S

S

A

A

A

A

G

S

T

S

L

L

K

K

H

E

V

V

T

T

M

M

E
|
E

L

L

G

G

G

G

K

K

S

S

A

P

L

L

L

I

V

I

F

A

A

E

D

D

A

A

D

N

L

L

D

D

D

L

A

A

V

A

S

D

A

I

A

A

M

M

L

M

A

A

N

N

F

F

Y

Y

T

S

Q

S

G

G

E

Q

I

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C
|
C

T

T

N

N

G

G

T

T

R

R

V

V

F

F

V

V

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P

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A

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K

V

F

C

K

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A

A

E

F

F

L

E

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H

K

K

A

I

K

L

A

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R

R

A

V

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G

A

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I

I

R

R

I

A

G

G

D

D

P

L

L

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A

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D

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D

T

T

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N

M

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G

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P

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L

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H

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N

V

V

M

L

K

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Y

V

I

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S

G

G

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K

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E

G

G

A

A

R

R

L

L

V

L

T

C

G

G

G

G

K

D

R

V

L

L

T

K

D

G

G

E

D

G

F

F

A

D

K

N

G

G

N

A

F

W

M

V

Q

A

P

P

T

T

I

V

F

F

A

T

D

D

V

C

L

T

D

D

Q

D

M

M

T

T

I

I

C

V

M

R

Q

E

E

E

I

I

F
|
F

G

G

P

P

V

V

M

M

S

S

V

I

L

L

P

S

F

Y

E

D

S

D

E

E

E

A

E

E

A

V

L

I

R

R

R

R

A

A

N

N

D

A

T

T

P

E

F

Y

G

G

L

L

A

A

A

A

G

G

V

V

M

V

T

T

R

P

D

D

I

L

S

N

R

R

A

A

H

H

R

R

V

I

A

I

A

H

K

Q

L

L

E

E

A

A

G

G

V

I

V

C

W

W

V

I

N

N

H

S

Y

W

N

G

L

E

T

S

P

P

I

A

P

E

M

M

P

P

F

V

G

G

G

G

S

Y

K

K

M

H

S

S

G

G

L

I

G

G

R

R

E

E

N

N

G

G

L

V

A

M

A

T

L

L

D

Q

F

S

Y

Y

T

T

E

Q

R

V

K

K

S

S

V

I

Y

Q

V

V

N

E

L

M

G

G

G

P

V

F

E

Q

S

S

P

I

Y

F

binding calcium ion: T33 (≠ S26), I34 (= I27), D100 (= D93), V187 (= V180)
binding nicotinamide-adenine-dinucleotide: I156 (= I149), G157 (= G150), A158 (= A151), W159 (= W152), K183 (= K176), E186 (= E179), G216 (= G208), G220 (= G212), T235 (= T227), G236 (= G228), G237 (≠ S229), S240 (≠ T232), K243 (≠ A235), E259 (= E250), C293 (= C284), F396 (= F387)

8vr1A Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (ctp bound)
53% identity, 99% coverage: 7:488/488 of query aligns to 4:488/488 of 8vr1A

query
sites
8vr1A

E

E

I

Q

S

Q

C

L

H

Y

I

I

A

H

G

G

R

K

R

F

I

V

P

A

G

A

-

T

S

S

G

G

D

K

A

T

L

F

S

E

S

T

I

I

N

N

P

P

A

A

N

T

G

G

E

E

T

V

L

L

A

A

T

T

G

V

N

Q

A

A

A

A

A

G

M

R

P

E

E

D

I

V

E

D

Q

R

A

A

I

V

E

K

A

S

A

A

R

Q

E

Q

A

G

R

Q

R

K

S

V

W

W

M

A

A

A

M

M

S

S

A

A

A

M

E

A

R

R

G

S

R

R

V

I

L

L

A

R

R

K

V

A

A

V

A

D

I

I

L

L

R

R

E

E

R

R

R

N

D

D

D

E

L

L

A

A

R

R

L

L

E

E

V

T

L

L

D

D

T

T

G

G

K

K

P

P

I

L

Q

S

E

E

A

T

P

A

A

A

A

V

D

D

I

I

D

V

S

T

A

G

A

A

D

D

C

V

F

L

E

E

F

Y

F

Y

A

A

G

G

L

L

T

I

Q

P

I

A

V

L

Q

E

G

G

E

S

H

Q

I

I

P

P

F

L

G

R

D

D

D

S

G

S

F

F

A

V

Y

Y

T

T

R

R

R

R

E

E

P

P

L

L

G

G

I

V

C

V

A

A

G

G

I

I

G

G

A

A

W
|
W

N

N

Y

Y

P

P

I

I

Q

Q

I

I

A

A

S

L

W

W

K

K

T

S

A

A

P

P

A

A

L

L

A

A

A

A

G

G

N

N

A

A

M

M

L

I

F

F

K

K

P

P

S

S

E

E

V

V

T

T

P

P

L

L

T

T

A

A

L

L

E

K

L

L

G

A

R

E

I

I

V

Y

E

R

E

E

A

A

G

G

A

L

P

P

P

D

G

G

L

V

L

F

S

N

I

V

L

L

P

P

G

G

-

I

G

G

R

A

E

E

T

T

G

G

E

Q

I

Y

L

L

S

T

T

E

H

H

P

P

A

D

I

I

A

A

K

K

I

I

S

S

V

F

T

T

G
|
G

S
x
G

V

V

P
x
A

T
x
S

G

G

K

K

A

K

V

V

M

M

A

A

-

N

S

S

A

A

A

A

G

S

T

S

L

L

K

K

H

E

V

V

T

T

M

M

E

E

L

L

G

G

G

G

K

K

S

S

A

P

L

L

L

I

V

I

F

A

A

E

D

D

A

A

D

N

L

L

D

D

D

L

A

A

V

A

S

D

A

I

A

A

M

M

L

M

A

A

N

N

F

F

Y

Y

T

S

Q

S

G

G

E

Q

I

V

C

C

T

T

N

N

G

G

T

T

R

R

V

V

F

F

V

V

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R

A

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K

V

F

C

K

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A

A

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F

F

L

E

E

H

K

K

A

I

K

L

A

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R

R

A

V

E

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A

R

I

I

R

R

I

A

G

G

D

D

P

L

L

F

D

A

P

D

E

D

T

T

Q

N

M

F

G

G

P

P

L

L

I

V

H

S

A

F

Q

P

H

H

L

R

D

Q

K

N

V

V

M

L

K

R

F

Y

V

I

E

E

N

S

G

G

V

K

R

S

E

E

G

G

A

A

R

R

L

L

V

L

T

C

G

G

G

G

K

D

R

V

L

L

T

K

D

G

G

E

D

G

F

F

A

D

K

N

G

G

N

A

F

W

M

V

Q

A

P

P

T

T

I

V

F

F

A

T

D

D

V

C

L

T

D

D

Q

D

M

M

T

T

I

I

C

V

M

R

Q

E

E
|
E

I

I

F
|
F

G

G

P

P

V

V

M

M

S

S

V

I

L

L

P

S

F

Y

E

D

S

D

E

E

E

A

E

E

A

V

L

I

R

R

R

R

A

A

N

N

D

A

T

T

P

E

F

Y

G

G

L

L

A

A

A

A

G

G

V

V

M

V

T

T

R

P

D

D

I

L

S

N

R

R

A

A

H

H

R

R

V

I

A

I

A

H

K

Q

L

L

E

E

A

A

G

G

V

I

V

C

W

W

V

I

N

N

H

S

Y

W

N

G

L

E

T

S

P

P

I

A

P

E

M

M

P

P

F

V

G

G

G

G

S

Y

K

K

M

H

S

S

G

G

L

I

G

G

R

R

E

E

N

N

G

G

L

V

A

M

A

T

L

L

D

Q

F

S

Y

Y

T

T

E

Q

R

V

K

K

S

S

V

I

Y

Q

V

V

N

E

L

M

G

G

G

P

V

F

E

Q

S

S

P

I

Y

F

binding cytidine-5'-triphosphate: W150 (= W152), G227 (= G228), G228 (≠ S229), A230 (≠ P231), S231 (≠ T232), E385 (= E385), F387 (= F387)

8vr0A Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (gmp bound)
53% identity, 99% coverage: 7:488/488 of query aligns to 4:488/488 of 8vr0A

query
sites
8vr0A

E

E

I

Q

S

Q

C

L

H

Y

I

I

A

H

G

G

R

K

R

F

I

V

P

A

G

A

-

T

S

S

G

G

D

K

A

T

L

F

S

E

S

T

I

I

N

N

P

P

A

A

N

T

G

G

E

E

T

V

L

L

A

A

T

T

G

V

N

Q

A

A

A

A

A

G

M

R

P

E

E

D

I

V

E

D

Q

R

A

A

I

V

E

K

A

S

A

A

R

Q

E

Q

A

G

R

Q

R

K

S

V

W

W

M

A

A

A

M

M

S

S

A

A

A

M

E

A

R

R

G

S

R

R

V

I

L

L

A

R

R

K

V

A

A

V

A

D

I

I

L

L

R

R

E

E

R

R

R

N

D

D

D

E

L

L

A

A

R

R

L

L

E

E

V

T

L

L

D

D

T

T

G

G

K

K

P

P

I

L

Q

S

E

E

A

T

P

A

A

A

A

V

D

D

I

I

D

V

S

T

A

G

A

A

D

D

C

V

F

L

E

E

F

Y

F

Y

A

A

G

G

L

L

T

I

Q

P

I

A

V

L

Q

E

G

G

E

S

H

Q

I

I

P

P

F

L

G

R

D

D

D

S

G

S

F

F

A

V

Y

Y

T

T

R

R

R

R

E

E

P

P

L

L

G

G

I

V

C

V

A

A

G

G

I

I

G

G

A

A

W
|
W

N

N

Y

Y

P

P

I

I

Q

Q

I

I

A

A

S

L

W

W

K

K

T

S

A

A

P

P

A

A

L

L

A

A

A

A

G

G

N

N

A

A

M

M

L

I

F

F

K

K

P

P

S

S

E

E

V

V

T

T

P

P

L

L

T

T

A

A

L

L

E

K

L

L

G

A

R

E

I

I

V

Y

E

R

E

E

A

A

G

G

A

L

P

P

P

D

G

G

L

V

L

F

S

N

I

V

L

L

P

P

G

G

-

I

G

G

R

A

E

E

T

T

G

G

E

Q

I

Y

L

L

S

T

T

E

H

H

P

P

A

D

I

I

A

A

K

K

I

I

S

S

V

F

T

T

G
|
G

S
x
G

V

V

P

A

T

S

G

G

K

K

A

K

V

V

M

M

A

A

-

N

S

S

A

A

A

A

G

S

T

S

L

L

K

K

H

E

V

V

T

T

M

M

E

E

L

L

G

G

G

G

K

K

S

S

A

P

L

L

L

I

V

I

F

A

A

E

D

D

A

A

D

N

L

L

D

D

D

L

A

A

V

A

S

D

A

I

A

A

M

M

L

M

A

A

N

N

F

F

Y

Y

T

S

Q

S

G

G

E

Q

I

V

C

C

T

T

N

N

G

G

T

T

R

R

V

V

F

F

V

V

E

P

R

A

P

K

V

F

C

K

E

A

A

E

F

F

L

E

E

H

K

K

A

I

K

L

A

E

R

R

A

V

E

G

A

R

I

I

R

R

I

A

G

G

D

D

P

L

L

F

D

A

P

D

E

D

T

T

Q

N

M

F

G

G

P

P

L

L

I

V

H

S

A

F

Q

P

H
|
H

L

R

D

Q

K

N

V

V

M

L

K

R

F

Y

V

I

E

E

N

S

G

G

V

K

R

S

E

E

G

G

A

A

R

R

L

L

V

L

T

C

G

G

G

G

K

D

R

V

L

L

T

K

D

G

G

E

D

G

F

F

A

D

K

N

G

G

N

A

F

W

M

V

Q

A

P

P

T

T

I

V

F

F

A

T

D

D

V

C

L

T

D

D

Q

D

M

M

T

T

I

I

C

V

M

R

Q

E

E
|
E

I

I

F
|
F

G

G

P

P

V

V

M

M

S

S

V

I

L

L

P

S

F

Y

E

D

S

D

E

E

E

A

E

E

A

V

L

I

R

R

R

R

A

A

N

N

D

A

T

T

P

E

F

Y

G

G

L

L

A

A

A

A

G

G

V

V

M

V

T

T

R

P

D

D

I

L

S

N

R

R

A

A

H

H

R

R

V

I

A

I

A

H

K

Q

L

L

E

E

A

A

G

G

V

I

V

C

W

W

V

I

N

N

H

S

Y

W

N

G

L

E

T

S

P

P

I

A

P

E

M

M

P

P

F

V

G

G

G

G

S

Y

K

K

M

H

S

S

G

G

L

I

G

G

R

R

E

E

N

N

G

G

L

V

A

M

A

T

L

L

D

Q

F

S

Y

Y

T

T

E

Q

R

V

K

K

S

S

V

I

Y

Q

V

V

N

E

L

M

G

G

G

P

V

F

E

Q

S

S

P

I

Y

F

binding guanosine-5'-monophosphate: W150 (= W152), G227 (= G228), G228 (≠ S229), H331 (= H331), E385 (= E385), F387 (= F387)

8vqzA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (cmp bound)
53% identity, 99% coverage: 7:488/488 of query aligns to 4:488/488 of 8vqzA

query
sites
8vqzA

E

E

I

Q

S

Q

C

L

H

Y

I

I

A

H

G

G

R

K

R

F

I

V

P

A

G

A

-

T

S

S

G

G

D

K

A

T

L

F

S

E

S

T

I

I

N

N

P

P

A

A

N

T

G

G

E

E

T

V

L

L

A

A

T

T

G

V

N

Q

A

A

A

A

A

G

M

R

P

E

E

D

I

V

E

D

Q

R

A

A

I

V

E

K

A

S

A

A

R

Q

E

Q

A

G

R

Q

R

K

S

V

W

W

M

A

A

A

M

M

S

S

A

A

A

M

E

A

R

R

G

S

R

R

V

I

L

L

A

R

R

K

V

A

A

V

A

D

I

I

L

L

R

R

E

E

R

R

R

N

D

D

D

E

L

L

A

A

R

R

L

L

E

E

V

T

L

L

D

D

T

T

G

G

K

K

P

P

I

L

Q

S

E

E

A

T

P

A

A

A

A

V

D

D

I

I

D

V

S

T

A

G

A

A

D

D

C

V

F

L

E

E

F

Y

F