SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_024750185.1 NCBI__GCF_000017845.1:WP_024750185.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

2gsaB Crystal structure of glutamate-1-semialdehyde aminomutase (aminotransferase, wild-type form) (see paper)
83% identity, 99% coverage: 7:433/433 of query aligns to 1:427/427 of 2gsaB

query
sites
2gsaB

L

F

K

K

T

T

T

I

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K

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D

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F

F

A

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Q

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K

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M

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P

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G

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S

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S

S

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P

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F

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K

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S

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V

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G

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G

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I

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F

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V

K

K

G

D

A

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Y

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I

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V

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D

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G

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N

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R

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Y

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I

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D

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Y

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T

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P

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I

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E

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A

A

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M

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A

A

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P

C

C

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A

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L

E

E

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N

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L

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A

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E

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M

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I

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M

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R

F

F

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V

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E

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C

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M

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R

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K

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F

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E

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E

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E

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A

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Y

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P

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L

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F

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A
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G

F

F

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T

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L

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A

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H

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T

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L

active site: V21 (= V27), Y144 (= Y150), E206 (= E212), D239 (= D245), M242 (= M248), K267 (= K273), A401 (= A407)
binding pyridoxal-5'-phosphate: G117 (= G123), T118 (= T124), Y144 (= Y150), E206 (= E212), N211 (= N217), D239 (= D245), V241 (= V247), M242 (= M248), K267 (= K273)

2gsaA Crystal structure of glutamate-1-semialdehyde aminomutase (aminotransferase, wild-type form) (see paper)
83% identity, 99% coverage: 7:433/433 of query aligns to 1:427/427 of 2gsaA

query
sites
2gsaA

L

F

K

K

T

T

T

I

K

K

S

S

E

D

D

E

I

I

F

F

A

A

A

A

A

A

Q

Q

K

K

L

L

M

M

P

P

G

G

G

G

V
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V

S

S

S

S

P

P

V

V

R

R

A

A

F

F

K

K

S

S

V

V

G

G

G

G

Q

Q

P

P

I

I

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V

F

F

D

D

H

R

V

V

K

K

G

D

A

A

Y

Y

I

A

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W

D

D

V

V

D

D

G

G

N

N

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R

Y

Y

I

I

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D

Y

Y

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V

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G

T

T

W

W

G

G

P

P

A

A

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I

C

C

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G

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A

A

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H

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P

E

E

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V

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I

A

E

A

A

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A

A

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M

D

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F

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A

A

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P

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C

L

A

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L

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E

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N

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L

A

A

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E

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M

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A

A

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M

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R

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F

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V

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E

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A

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C

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M

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A

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T

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R

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D

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I

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I

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K

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F

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E

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H

H

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A

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D

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M

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A

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P

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G

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T

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A

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T

T

L

L

T

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A

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P

Y

Y

N

N

D

D

L

L

E

E

A

A

V

V

K

K

A

A

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L

F

F

A

A

E

E

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N

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P

D

G

E

E

I

I

A

A

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I

I

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E
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E

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P

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G

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F

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I

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V

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P

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D

G

A

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F

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E

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D

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G

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P

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L

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I

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K

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T

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E

E

L

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Y

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E

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D

A

A

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K

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K

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D

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L

A

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K

F

F

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R

F

F

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R

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M

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G

G

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Y

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L

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A

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Q

F

F

E

E

A
|
A

G

G

F

F

T

T

S

S

L

L

A

A

H

H

T

T

S

E

E

E

D

D

I

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E

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K

A

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A

A

A

A

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T

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A

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L

active site: V21 (= V27), Y144 (= Y150), E206 (= E212), D239 (= D245), M242 (= M248), K267 (= K273), A401 (= A407)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G117 (= G123), T118 (= T124), Y144 (= Y150), H145 (= H151), G146 (= G152), N211 (= N217), D239 (= D245), V241 (= V247), K267 (= K273)

3usfA Crystal structure of dava-4
83% identity, 99% coverage: 7:433/433 of query aligns to 1:427/427 of 3usfA

query
sites
3usfA

L

F

K

K

T

T

T

I

K

K

S

S

E

D

D

E

I

I

F

F

A

A

A

A

A

A

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Q

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K

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L

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M

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P

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G

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V

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S
|
S

P

P

V
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V

R

R

A

A

F

F

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K

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S

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V

G

G

G

G

Q

Q

P

P

I

I

V

V

F

F

D

D

H

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V

V

K

K

G

D

A

A

Y

Y

I

A

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W

D

D

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V

D

D

G

G

N

N

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R

Y

Y

I

I

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D

Y

Y

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V

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T

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W

G

G

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A

A

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I

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G

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A

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P

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C

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A

L

L

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E

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L

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E

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M

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D

A

A

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E

E

M

M

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V

R

R

F

F

V

V

N

N

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S

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G

T
|
T

E

E

A

A

C

C

M

M

S

A

V

V

L

L

R

R

L

I

M

M

R

R

A

A

F

Y

T

T

G

G

R

R

D

D

K

K

I

I

I

I

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K

F

F

Q

E

G

G

C

C

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|
Y

H

H

G

G

H

H

A

A

D

D

M

M

F

F

L

L

V

V

Q

K

A

A

G

G

S
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S

G

G

V

V

A

A

T

T

L

L

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G

L

L

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P

D

S

S

S

P

P

G

G

V

V

P

P

K

K

T

K

T

T

T

T

A

A

N

N

T

T

L

L

T

T

A

T

P

P

Y

Y

N

N

D

D

L

L

E

E

A

A

V

V

K

K

A

A

L

L

F

F

A

A

E

E

N

N

P

P

D

G

E

E

I

I

A

A

G

G

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V

I

I

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L

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E

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P

V

I

V

V

G

G

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N

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S

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G

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F

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P

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D

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A

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F

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F

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M

Y

Y

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Q

A

A

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G

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T

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L

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S

G

G

N

N

P

P

L

L

A

A

M

M

T

T

A

A

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I

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T

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L

E

E

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A

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Y

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L

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A

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E

A
|
A

G

G

F

F

T

T

S

S

L

L

A

A

H

H

T

T

S

E

E

E

D

D

I

I

E

D

K

A

T

T

L

L

A

A

A

A

A

A

K

R

D

T

V

V

L

M

S

S

N

A

L

L

active site: V21 (= V27), Y144 (= Y150), D239 (= D245), M242 (= M248), K267 (= K273), A401 (= A407)
binding (4s)-4,5-diaminopentanoic acid: S23 (= S29), V25 (= V31), S157 (= S163), K267 (= K273), E400 (= E406)
binding (5-hydroxy-4,6-dimethylpyridin-3-yl)methyl dihydrogen phosphate: G117 (= G123), T118 (= T124), Y144 (= Y150), N211 (= N217), D239 (= D245), V241 (= V247), K267 (= K273)

3fq7A Gabaculine complex of gsam (see paper)
83% identity, 99% coverage: 7:433/433 of query aligns to 1:427/427 of 3fq7A

query
sites
3fq7A

L

F

K

K

T

T

T

I

K

K

S

S

E

D

D

E

I

I

F

F

A

A

A

A

A

A

Q

Q

K

K

L

L

M

M

P

P

G

G

G

G

V
|
V

S

S

S
|
S

P

P

V
|
V

R

R

A

A

F

F

K

K

S

S

V

V

G

G

G

G

Q

Q

P

P

I

I

V

V

F

F

D

D

H

R

V

V

K

K

G

D

A

A

Y

Y

I

A

W

W

D

D

V

V

D

D

G

G

N

N

Q

R

Y

Y

I

I

D

D

Y

Y

V

V

G

G

T

T

W
|
W

G

G

P

P

A

A

I

I

C

C

G

G

H

H

A

A

H

H

P

P

E

E

V

V

I

I

A

E

A

A

L

L

H

K

D

V

A

A

L

M

D

E

R

K

G

G

T

T

S

S

F

F

G

G

A

A

P

P

C

C

L

A

L

L

E

E

N

N

V

V

L

L

A

A

E

E

M

M

V

V

I

N

D

D

A

A

V

V

P

P

S

S

I

I

E

E

M

M

V

V

R

R

F

F

V

V

N

N

S

S

G
|
G

T
|
T

E

E

A

A

C

C

M

M

S

A

V

V

L

L

R

R

L

I

M

M

R

R

A

A

F

Y

T

T

G

G

R

R

D

D

K

K

I

I

I

I

K

K

F

F

Q

E

G

G

C

C

Y
|
Y

H
|
H

G

G

H

H

A

A

D

D

M

M

F

F

L

L

V

V

Q

K

A

A

G

G

S

S

G

G

V

V

A

A

T

T

L

L

G

G

L

L

P

P

D

S

S

S

P

P

G

G

V

V

P

P

K

K

T

K

T

T

T

T

A

A

N

N

T

T

L

L

T

T

A

T

P

P

Y

Y

N

N

D

D

L

L

E

E

A

A

V

V

K

K

A

A

L

L

F

F

A

A

E

E

N

N

P

P

D

G

E

E

I

I

A

A

G

G

V

V

I

I

L

L

E
|
E

P

P

V

I

V

V

G

G

N
|
N

S

S

G

G

F

F

V

I

V

V

P

P

D

D

G

A

G

G

F

F

L

L

Q

E

G

G

L

L

R

R

E

E

L

I

T

T

N

L

E

E

Y

H

G

D

A

A

L

L

L

L

V

V

F

F

D
|
D

E

E

V
|
V

M
|
M

T

T

G

G

F

F

R

R

L

I

S

A

Y

Y

G

G

G

G

A

V

Q

Q

E

E

K

K

F

F

G

G

V

V

T

T

P

P

D

D

L

L

T

T

T

T

L

L

G

G

K
|
K

V

I

I

I

G

G

G

G

G

G

L

L

P

P

V

V

G

G

A

A

Y

Y

G

G

G

G

R

K

K

R

D

E

I

I

M

M

S

Q

M

L

V

V

A

A

P

P

A

A

G

G

P

P

M

M

Y

Y

Q

Q

A

A

G
|
G

T
|
T

L

L

S

S

G

G

N

N

P

P

L

L

A

A

M

M

T

T

A

A

G

G

I

I

K

K

T

T

L

L

E

E

L

L

L

L

Q

R

K

Q

P

P

G

G

T

T

Y

Y

N

E

Q

Y

L

L

E

D

Q

Q

I

I

T

T

Q

K

Q

R

L

L

S

S

E

D

G

G

L

L

L

L

K

A

I

I

A

A

K

Q

E

E

A

T

G

G

H

H

Q

A

V

A

C

C

G

G

G

G

Y

Q

I

V

G

S

A

G

M

M

F

F

G

G

L

F

F

F

F

F

T

T

E

E

G

G

P

P

V

V

R

H

N

N

Y

Y

D

E

D

D

A

A

K

K

K

K

S

S

D

D

L

L

A

Q

K

K

F

F

G

S

R

R

F

F

H

H

R

R

G

G

M

M

L

L

E

E

K

Q

G

G

V

I

Y

Y

L

L

A

A

P

P

S

S

Q

Q

F

F

E
|
E

A
|
A

G

G

F

F

T

T

S

S

L

L

A

A

H

H

T

T

S

E

E

E

D

D

I

I

E

D

K

A

T

T

L

L

A

A

A

A

A

A

K

R

D

T

V

V

L

M

S

S

N

A

L

L

active site: V21 (= V27), Y144 (= Y150), D239 (= D245), M242 (= M248), K267 (= K273), A401 (= A407)
binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: S23 (= S29), V25 (= V31), W61 (= W67), G117 (= G123), T118 (= T124), Y144 (= Y150), H145 (= H151), E206 (= E212), N211 (= N217), D239 (= D245), V241 (= V247), M242 (= M248), K267 (= K273), G298 (= G304), T299 (= T305), E400 (= E406)

2hp2A Inter-subunit signaling in gsam (see paper)
83% identity, 99% coverage: 7:433/433 of query aligns to 1:427/427 of 2hp2A

query
sites
2hp2A

L

F

K

K

T

T

T

I

K

K

S

S

E

D

D

E

I

I

F

F

A

A

A

A

A

A

Q

Q

K

K

L

L

M

M

P

P

G

G

G

G

V
|
V

S

S

S
|
S

P

P

V

V

R

R

A

A

F

F

K

K

S

S

V

V

G

G

G

G

Q

Q

P

P

I

I

V

V

F

F

D

D

H

R

V

V

K

K

G

D

A

A

Y

Y

I

A

W

W

D

D

V

V

D

D

G

G

N

N

Q

R

Y

Y

I

I

D

D

Y

Y

V

V

G

G

T

T

W

W

G

G

P

P

A

A

I

I

C

C

G

G

H

H

A

A

H

H

P

P

E

E

V

V

I

I

A

E

A

A

L

L

H

K

D

V

A

A

L

M

D

E

R

K

G

G

T

T

S

S

F

F

G

G

A

A

P

P

C

C

L

A

L

L

E

E

N

N

V

V

L

L

A

A

E

E

M

M

V

V

I

N

D

D

A

A

V

V

P

P

S

S

I

I

E

E

M

M

V

V

R

R

F

F

V

V

N

N

S

S

G
|
G

T
|
T

E

E

A

A

C

C

M

M

S

A

V

V

L

L

R

R

L

I

M

M

R

R

A

A

F

Y

T

T

G

G

R

R

D

D

K

K

I

I

I

I

K

K

F

F

Q

E

G

G

C

C

Y
|
Y

H
|
H

G

G

H

H

A

A

D

D

M

M

F

F

L

L

V

V

Q

K

A

A

G

G

S

S

G

G

V

V

A

A

T

T

L

L

G

G

L

L

P

P

D

S

S

S

P

P

G

G

V

V

P

P

K

K

T

K

T

T

T

T

A

A

N

N

T

T

L

L

T

T

A

T

P

P

Y

Y

N

N

D

D

L

L

E

E

A

A

V

V

K

K

A

A

L

L

F

F

A

A

E

E

N

N

P

P

D

G

E

E

I

I

A

A

G

G

V

V

I

I

L

L

E
|
E

P

P

V

I

V

V

G

G

N
|
N

S

S

G

G

F

F

V

I

V

V

P

P

D

D

G

A

G

G

F

F

L

L

Q

E

G

G

L

L

R

R

E

E

L

I

T

T

N

L

E

E

Y

H

G

D

A

A

L

L

L

L

V

V

F

F

D
|
D

E

E

V
|
V

M
|
M

T

T

G

G

F

F

R

R

L

I

S

A

Y

Y

G

G

G

G

A

V

Q

Q

E

E

K

K

F

F

G

G

V

V

T

T

P

P

D

D

L

L

T

T

T

T

L

L

G

G

K
|
K

V

I

I

I

G

G

G

G

G

G

L

L

P

P

V

V

G

G

A

A

Y

Y

G

G

G

G

R

K

K

R

D

E

I

I

M

M

S

Q

M

L

V

V

A

A

P

P

A

A

G

G

P

P

M

M

Y

Y

Q

Q

A

A

G
|
G

T
|
T

L

L

S

S

G

G

N

N

P

P

L

L

A

A

M

M

T

T

A

A

G

G

I

I

K

K

T

T

L

L

E

E

L

L

L

L

Q

R

K

Q

P

P

G

G

T

T

Y

Y

N

E

Q

Y

L

L

E

D

Q

Q

I

I

T

T

Q

K

Q

R

L

L

S

S

E

D

G

G

L

L

L

L

K

A

I

I

A

A

K

Q

E

E

A

T

G

G

H

H

Q

A

V

A

C

C

G

G

G

G

Y

Q

I

V

G

S

A

G

M

M

F

F

G

G

L

F

F

F

F

F

T

T

E

E

G

G

P

P

V

V

R

H

N

N

Y

Y

D

E

D

D

A

A

K

K

K

K

S

S

D

D

L

L

A

Q

K

K

F

F

G

S

R

R

F

F

H

H

R

R

G

G

M

M

L

L

E

E

K

Q

G

G

V

I

Y

Y

L

L

A

A

P

P

S

S

Q

Q

F

F

E

E

A
|
A

G

G

F

F

T

T

S

S

L

L

A

A

H

H

T

T

S

E

E

E

D

D

I

I

E

D

K

A

T

T

L

L

A

A

A

A

A

A

K

R

D

T

V

V

L

M

S

S

N

A

L

L

active site: V21 (= V27), Y144 (= Y150), D239 (= D245), M242 (= M248), K267 (= K273), A401 (= A407)
binding (4s)-4,5-diaminopentanoic acid: G298 (= G304), T299 (= T305)
binding (4r)-5-amino-4-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]pentanoic acid: S23 (= S29), G117 (= G123), T118 (= T124), Y144 (= Y150), H145 (= H151), E206 (= E212), N211 (= N217), D239 (= D245), V241 (= V247), M242 (= M248), K267 (= K273)
binding pyridoxal-5'-phosphate: G298 (= G304), T299 (= T305)

2hp1A Inter-subunit signaling in gsam (see paper)
83% identity, 99% coverage: 7:433/433 of query aligns to 1:427/427 of 2hp1A

query
sites
2hp1A

L

F

K

K

T

T

T

I

K

K

S

S

E

D

D

E

I

I

F

F

A

A

A

A

A

A

Q

Q

K

K

L

L

M

M

P

P

G

G

G

G

V
|
V

S

S

S
|
S

P

P

V
|
V

R

R

A

A

F

F

K

K

S

S

V

V

G

G

G

G

Q

Q

P

P

I

I

V

V

F

F

D

D

H

R

V

V

K

K

G

D

A

A

Y

Y

I

A

W

W

D

D

V

V

D

D

G

G

N

N

Q

R

Y

Y

I

I

D

D

Y

Y

V

V

G

G

T

T

W
|
W

G

G

P

P

A

A

I

I

C

C

G

G

H

H

A

A

H

H

P

P

E

E

V

V

I

I

A

E

A

A

L

L

H

K

D

V

A

A

L

M

D

E

R

K

G

G

T

T

S

S

F

F

G

G

A

A

P

P

C

C

L

A

L

L

E

E

N

N

V

V

L

L

A

A

E

E

M

M

V

V

I

N

D

D

A

A

V

V

P

P

S

S

I

I

E

E

M

M

V

V

R

R

F

F

V

V

N

N

S
|
S

G
|
G

T
|
T

E

E

A

A

C

C

M

M

S

A

V

V

L

L

R

R

L

I

M

M

R

R

A

A

F

Y

T

T

G

G

R

R

D

D

K

K

I

I

I

I

K

K

F

F

Q

E

G

G

C

C

Y
|
Y

H
|
H

G
|
G

H

H

A

A

D

D

M

M

F

F

L

L

V

V

Q

K

A

A

G

G

S

S

G

G

V

V

A

A

T

T

L

L

G

G

L

L

P

P

D

S

S

S

P

P

G

G

V

V

P

P

K

K

T

K

T

T

T

T

A

A

N

N

T

T

L

L

T

T

A

T

P

P

Y

Y

N

N

D

D

L

L

E

E

A

A

V

V

K

K

A

A

L

L

F

F

A

A

E

E

N

N

P

P

D

G

E

E

I

I

A

A

G

G

V

V

I

I

L

L

E
|
E

P

P

V

I

V

V

G

G

N
|
N

S

S

G

G

F

F

V

I

V

V

P

P

D

D

G

A

G

G

F

F

L

L

Q

E

G

G

L

L

R

R

E

E

L

I

T

T

N

L

E

E

Y

H

G

D

A

A

L

L

L

L

V

V

F

F

D
|
D

E

E

V
|
V

M
|
M

T

T

G

G

F

F

R

R

L

I

S

A

Y

Y

G

G

G

G

A

V

Q

Q

E

E

K

K

F

F

G

G

V

V

T

T

P

P

D

D

L

L

T

T

T

T

L

L

G

G

K
|
K

V

I

I

I

G

G

G

G

G

G

L

L

P

P

V

V

G

G

A

A

Y

Y

G

G

G

G

R

K

K

R

D

E

I

I

M

M

S

Q

M

L

V

V

A

A

P

P

A

A

G

G

P

P

M

M

Y

Y

Q

Q

A

A

G

G

T

T

L

L

S

S

G

G

N

N

P

P

L

L

A

A

M

M

T

T

A

A

G

G

I

I

K

K

T

T

L

L

E

E

L

L

L

L

Q

R

K

Q

P

P

G

G

T

T

Y

Y

N

E

Q

Y

L

L

E

D

Q

Q

I

I

T

T

Q

K

Q

R

L

L

S

S

E

D

G

G

L

L

L

L

K

A

I

I

A

A

K

Q

E

E

A

T

G

G

H

H

Q

A

V

A

C

C

G

G

G

G

Y

Q

I

V

G

S

A

G

M

M

F

F

G

G

L

F

F

F

F

F

T

T

E

E

G

G

P

P

V

V

R

H

N

N

Y

Y

D

E

D

D

A

A

K

K

K

K

S

S

D

D

L

L

A

Q

K

K

F

F

G

S

R

R

F

F

H

H

R

R

G

G

M

M

L

L

E

E

K

Q

G

G

V

I

Y

Y

L

L

A

A

P

P

S

S

Q

Q

F

F

E
|
E

A
|
A

G

G

F

F

T

T

S

S

L

L

A

A

H

H

T

T

S

E

E

E

D

D

I

I

E

D

K

A

T

T

L

L

A

A

A

A

A

A

K

R

D

T

V

V

L

M

S

S

N

A

L

L

active site: V21 (= V27), Y144 (= Y150), D239 (= D245), M242 (= M248), K267 (= K273), A401 (= A407)
binding (4s)-4-amino-5-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]pentanoic acid: S23 (= S29), V25 (= V31), W61 (= W67), S116 (= S122), G117 (= G123), T118 (= T124), Y144 (= Y150), H145 (= H151), G146 (= G152), E206 (= E212), N211 (= N217), D239 (= D245), V241 (= V247), M242 (= M248), K267 (= K273), E400 (= E406)

2hozA Inter-subunit signaling in gsam (see paper)
83% identity, 99% coverage: 7:433/433 of query aligns to 1:427/427 of 2hozA

query
sites
2hozA

L

F

K

K

T

T

T

I

K

K

S

S

E

D

D

E

I

I

F

F

A

A

A

A

A

A

Q

Q

K

K

L

L

M

M

P

P

G

G

G

G

V
|
V

S

S

S

S

P

P

V

V

R

R

A

A

F

F

K

K

S

S

V

V

G

G

G

G

Q

Q

P

P

I

I

V

V

F

F

D

D

H

R

V

V

K

K

G

D

A

A

Y

Y

I

A

W

W

D

D

V

V

D

D

G

G

N

N

Q

R

Y

Y

I

I

D

D

Y

Y

V

V

G

G

T

T

W

W

G

G

P

P

A

A

I

I

C

C

G

G

H

H

A

A

H

H

P

P

E

E

V

V

I

I

A

E

A

A

L

L

H

K

D

V

A

A

L

M

D

E

R

K

G

G

T

T

S

S

F

F

G

G

A

A

P

P

C

C

L

A

L

L

E

E

N

N

V

V

L

L

A

A

E

E

M

M

V

V

I

N

D

D

A

A

V

V

P

P

S

S

I

I

E

E

M

M

V

V

R

R

F

F

V

V

N

N

S

S

G
|
G

T
|
T

E

E

A

A

C

C

M

M

S

A

V

V

L

L

R

R

L

I

M

M

R

R

A

A

F

Y

T

T

G

G

R

R

D

D

K

K

I

I

I

I

K

K

F

F

Q
x
E

G

G

C

C

Y
|
Y

H
|
H

G
|
G

H

H

A

A

D

D

M

M

F

F

L

L

V

V

Q

K

A

A

G
|
G

S
|
S

G

G

V

V

A

A

T

T

L

L

G

G

L

L

P

P

D

S

S

S

P

P

G

G

V

V

P

P

K

K

T

K

T

T

T

T

A

A

N

N

T

T

L

L

T

T

A

T

P
|
P

Y

Y

N

N

D

D

L

L

E

E

A

A

V

V

K

K

A

A

L

L

F

F

A

A

E

E

N

N

P

P

D

G

E

E

I

I

A

A

G

G

V

V

I

I

L

L

E
|
E

P

P

V

I

V

V

G

G

N
|
N

S

S

G

G

F

F

V

I

V

V

P

P

D

D

G

A

G

G

F

F

L

L

Q

E

G

G

L

L

R

R

E

E

L

I

T

T

N

L

E

E

Y

H

G

D

A

A

L

L

L

L

V

V

F

F

D
|
D

E

E

V

V

M
|
M

T

T

G

G

F

F

R

R

L

I

S

A

Y

Y

G

G

G

G

A

V

Q

Q

E

E

K

K

F

F

G

G

V

V

T

T

P

P

D

D

L

L

T

T

T

T

L

L

G

G

K
|
K

V

I

I

I

G

G

G

G

G

G

L

L

P

P

V

V

G

G

A

A

Y

Y

G

G

G

G

R

K

K

R

D

E

I

I

M

M

S

Q

M

L

V

V

A

A

P

P

A

A

G

G

P

P

M

M

Y

Y

Q

Q

A

A

G
|
G

T
|
T

L

L

S

S

G

G

N

N

P

P

L

L

A

A

M

M

T

T

A

A

G

G

I

I

K

K

T

T

L

L

E

E

L

L

L

L

Q

R

K

Q

P

P

G

G

T

T

Y

Y

N

E

Q

Y

L

L

E

D

Q

Q

I

I

T

T

Q

K

Q

R

L

L

S

S

E

D

G

G

L

L

L

L

K

A

I

I

A

A

K

Q

E

E

A

T

G

G

H

H

Q

A

V

A

C

C

G

G

G

G

Y

Q

I

V

G

S

A

G

M

M

F

F

G

G

L

F

F

F

F

F

T

T

E

E

G

G

P

P

V

V

R

H

N
|
N

Y

Y

D
x
E

D

D

A

A

K
|
K

K

K

S

S

D

D

L

L

A

Q

K

K

F

F

G

S

R

R

F

F

H

H

R

R

G

G

M

M

L

L

E

E

K

Q

G

G

V

I

Y

Y

L

L

A

A

P

P

S

S

Q

Q

F

F

E

E

A
|
A

G

G

F

F

T

T

S

S

L

L

A

A

H

H

T

T

S

E

E

E

D

D

I

I

E

D

K

A

T

T

L

L

A

A

A

A

A

A

K

R

D

T

V

V

L

M

S

S

N

A

L

L

active site: V21 (= V27), Y144 (= Y150), D239 (= D245), M242 (= M248), K267 (= K273), A401 (= A407)
binding (4s)-4,5-diaminopentanoic acid: E141 (≠ Q147), G156 (= G162), S157 (= S163), P182 (= P188), N368 (= N374), E370 (≠ D376), K373 (= K379)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G117 (= G123), T118 (= T124), Y144 (= Y150), H145 (= H151), G146 (= G152), E206 (= E212), N211 (= N217), D239 (= D245), G298 (= G304), T299 (= T305)

3fqaA Gabaculien complex of gabaculine resistant gsam version (see paper)
83% identity, 98% coverage: 8:433/433 of query aligns to 1:426/426 of 3fqaA

query
sites
3fqaA

K

K

T

T

T

I

K

K

S

S

E

D

D

E

I

I

F

F

A

A

A

A

A

A

Q

Q

K

K

L

L

M

M

P

P

G

G

G

G

V
|
V

S

S

S
|
S

P

P

V

V

R
|
R

A

A

F

F

K

K

S

S

V

V

G

G

G

G

Q

Q

P

P

I

I

V

V

F

F

D

D

H

R

V

V

K

K

G

D

A

A

Y

Y

I

A

W

W

D

D

V

V

D

D

G

G

N

N

Q

R

Y

Y

I

I

D

D

Y

Y

V

V

G

G

T

T

W
|
W

G

G

P

P

A

A

I

I

C

C

G

G

H

H

A

A

H

H

P

P

E

E

V

V

I

I

A

E

A

A

L

L

H

K

D

V

A

A

L

M

D

E

R

K

G

G

T

T

S

S

F

F

G

G

A

A

P

P

C

C

L

A

L

L

E

E

N

N

V

V

L

L

A

A

E

E

M

M

V

V

I

N

D

D

A

A

V

V

P

P

S

S

I

I

E

E

M

M

V

V

R

R

F

F

V

V

N

N

S

S

G
|
G

T
|
T

E

E

A

A

C

C

M

M

S

A

V

V

L

L

R

R

L

L

M

M

R

R

A

A

F

Y

T

T

G

G

R

R

D

D

K

K

I

I

I

I

K

K

F

F

Q

E

G

G

C

C

Y
|
Y

H

H

G

G

H

H

A

A

D

D

M

M

F

F

L

L

V

V

Q

K

A

A

G

G

S

S

G

G

V

V

A

A

T

T

L

L

G

G

L

L

P

P

D

S

S

S

P

P

G

G

V

V

P

P

K

K

T

K

T

T

T

T

A

A

N

N

T

T

L

L

T

T

A

T

P

P

Y

Y

N

N

D

D

L

L

E

E

A

A

V

V

K

K

A

A

L

L

F

F

A

A

E

E

N

N

P

P

D

G

E

E

I

I

A

A

G

G

V

V

I

I

L

L

E
|
E

P

P

V

I

V

V

G

G

N
|
N

S

S

G

G

F

F

V

I

V

V

P

P

D

D

G

A

G

G

F

F

L

L

Q

E

G

G

L

L

R

R

E

E

L

I

T

T

N

L

E

E

Y

H

G

D

A

A

L

L

L

L

V

V

F

F

D
|
D

E

E

V
|
V

M
x
I

T

T

G

G

F

F

R

R

L

I

S

A

Y

Y

G

G

G

G

A

V

Q

Q

E

E

K

K

F

F

G

G

V

V

T

T

P

P

D

D

L

L

T

T

T

T

L

L

G

G

K
|
K

V

I

I

I

G

G

G

G

G

G

L

L

P

P

V

V

G

G

A

A

Y

Y

G

G

G

G

R

K

K

R

D

E

I

I

M

M

S

Q

M

L

V

V

A

A

P

P

A

A

G

G

P

P

M

M

Y

Y

Q

Q

A

A

G

G

T

T

L

L

S

S

G

G

N

N

P

P

L

L

A

A

M

M

T

T

A

A

G

G

I

I

K

K

T

T

L

L

E

E

L

L

L

L

Q

R

K

Q

P

P

G

G

T

T

Y

Y

N

E

Q

Y

L

L

E

D

Q

Q

I

I

T

T

Q

K

Q

R

L

L

S

S

E

D

G

G

L

L

L

L

K

A

I

I

A

A

K

Q

E

E

A

T

G

G

H

H

Q

A

V

A

C

C

G

G

G

G

Y

Q

I

V

G

S

A

G

M

M

F

F

G

G

L

F

F

F

F

F

T

T

E

E

G

G

P

P

V

V

R

H

N

N

Y

Y

D

E

D

D

A

A

K

K

K

K

S

S

D

D

L

L

A

Q

K

K

F

F

G

S

R

R

F

F

H

H

R

R

G

G

M

M

L

L

E

E

K

Q

G

G

V

I

Y

Y

L

L

A

A

P

P

S

S

Q

Q

F

F

E

E

A
|
A

G

G

F

F

T

T

S

S

L

L

A

A

H

H

T

T

S

E

E

E

D

D

I

I

E

D

K

A

T

T

L

L

A

A

A

A

A

A

K

R

D

T

V

V

L

M

S

S

N

A

L

L

active site: V20 (= V27), Y143 (= Y150), D238 (= D245), I241 (≠ M248), K266 (= K273), A400 (= A407)
binding 3-aminobenzoic acid: S22 (= S29), R25 (= R32), W60 (= W67)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G116 (= G123), T117 (= T124), Y143 (= Y150), E205 (= E212), N210 (= N217), D238 (= D245), V240 (= V247), I241 (≠ M248)

3usfB Crystal structure of dava-4
78% identity, 99% coverage: 7:433/433 of query aligns to 1:402/402 of 3usfB

query
sites
3usfB

L

F

K

K

T

T

T

I

K

K

S

S

E

D

D

E

I

I

F

F

A

A

A

A

A

A

Q

Q

K

K

L

L

M

M

P

P

G

G

G

G

V
|
V

S

S

S

S

P

P

V

V

R

R

A

A

F

F

K

K

S

S

V

V

G

G

G

G

Q

Q

P

P

I

I

V

V

F

F

D

D

H

R

V

V

K

K

G

D

A

A

Y

Y

I

A

W

W

D

D

V

V

D

D

G

G

N

N

Q

R

Y

Y

I

I

D

D

Y

Y

V

V

G

G

T

T

W

W

G

G

P

P

A

A

I

I

C

C

G

G

H

H

A

A

H

H

P

P

E

E

V

V

I

I

A

E

A

A

L

L

H

K

D

V

A

A

L

M

D

E

R

K

G

G

T

T

S

S

F

F

G

G

A

A

P

P

C

C

L

A

L

L

E

E

N

N

V

V

L

L

A

A

E

E

M

M

V

V

I

N

D

D

A

A

V

V

P

P

S

S

I

I

E

E

M

M

V

V

R

R

F

F

V

V

N

N

S

S

G
|
G

T
|
T

E

E

A

A

C

C

M

M

S

A

V

V

L

L

R

R

L

I

M

M

R

R

A

A

F

Y

T

T

G

G

R

R

D

D

K

K

I

I

I

I

K

K

F

F

Q

E

G

G

C

C

Y
|
Y

H

H

G

G

H

H

A

A

D

D

M

-

F

-

L

-

V

-

Q

-

A

-

G

-

S

-

G

-

V

-

A

-

T

-

L

-

G

-

L

-

P

-

D

-

S

-

P

-

G

-

V

-

P

-

K

-

T

-

T

-

T

T

A

A

N

N

T

T

L

L

T

T

A

T

P

P

Y

Y

N

N

D

D

L

L

E

E

A

A

V

V

K

K

A

A

L

L

F

F

A

A

E

E

N

N

P

P

D

G

E

E

I

I

A

A

G

G

V

V

I

I

L

L

E
|
E

P

P

V

I

V

V

G

G

N
|
N

S

S

G

G

F

F

V

I

V

V

P

P

D

D

G

A

G

G

F

F

L

L

Q

E

G

G

L

L

R

R

E

E

L

I

T

T

N

L

E

E

Y

H

G

D

A

A

L

L

L

L

V

V

F

F

D
|
D

E

E

V
|
V

M
|
M

T

T

G

G

F

F

R

R

L

I

S

A

Y

Y

G

G

G

G

A

V

Q

Q

E

E

K

K

F

F

G

G

V

V

T

T

P

P

D

D

L

L

T

T

T

T

L

L

G

G

K
|
K

V

I

I

I

G

G

G

G

G

G

L

L

P

P

V

V

G

G

A

A

Y

Y

G

G

G

G

R

K

K

R

D

E

I

I

M

M

S

Q

M

L

V

V

A

A

P

P

A

A

G

G

P

P

M

M

Y

Y

Q

Q

A

A

G
|
G

T
|
T

L

L

S

S

G

G

N

N

P

P

L

L

A

A

M

M

T

T

A

A

G

G

I

I

K

K

T

T

L

L

E

E

L

L

L

L

Q

R

K

Q

P

P

G

G

T

T

Y

Y

N

E

Q

Y

L

L

E

D

Q

Q

I

I

T

T

Q

K

Q

R

L

L

S

S

E

D

G

G

L

L

L

L

K

A

I

I

A

A

K

Q

E

E

A

T

G

G

H

H

Q

A

V

A

C

C

G

G

G

G

Y

Q

I

V

G

S

A

G

M

M

F

F

G

G

L

F

F

F

F

F

T

T

E

E

G

G

P

P

V

V

R

H

N

N

Y

Y

D

E

D

D

A

A

K

K

K

K

S

S

D

D

L

L

A

Q

K

K

F

F

G

S

R

R

F

F

H

H

R

R

G

G

M

M

L

L

E

E

K

Q

G

G

V

I

Y

Y

L

L

A

A

P

P

S

S

Q

Q

F

F

E

E

A
|
A

G

G

F

F

T

T

S

S

L

L

A

A

H

H

T

T

S

E

E

E

D

D

I

I

E

D

K

A

T

T

L

L

A

A

A

A

A

A

K

R

D

T

V

V

L

M

S

S

N

A

L

L

active site: V21 (= V27), Y144 (= Y150), E181 (= E212), D214 (= D245), M217 (= M248), K242 (= K273), A376 (= A407)
binding (4s)-4,5-diaminopentanoic acid: G273 (= G304), T274 (= T305)
binding (5-hydroxy-4,6-dimethylpyridin-3-yl)methyl dihydrogen phosphate: G117 (= G123), T118 (= T124), Y144 (= Y150), E181 (= E212), N186 (= N217), D214 (= D245), V216 (= V247), M217 (= M248), K242 (= K273)

2hp1B Inter-subunit signaling in gsam (see paper)
77% identity, 99% coverage: 7:433/433 of query aligns to 1:398/398 of 2hp1B

query
sites
2hp1B

L

F

K

K

T

T

T

I

K

K

S

S

E

D

D

E

I

I

F

F

A

A

A

A

A

A

Q

Q

K

K

L

L

M

M

P

P

G

G

G

G

V
|
V

S

S

S
|
S

P

P

V
|
V

R
|
R

A

A

F

F

K

K

S

S

V

V

G

G

G

G

Q

Q

P

P

I

I

V

V

F

F

D

D

H

R

V

V

K

K

G

D

A

A

Y

Y

I

A

W

W

D

D

V

V

D

D

G

G

N

N

Q

R

Y

Y

I

I

D

D

Y

Y

V

V

G

G

T

T

W
|
W

G

G

P

P

A

A

I

I

C

C

G

G

H

H

A

A

H

H

P

P

E

E

V

V

I

I

A

E

A

A

L

L

H

K

D

V

A

A

L

M

D

E

R

K

G

G

T

T

S

S

F

F

G

G

A

A

P

P

C

C

L

A

L

L

E

E

N

N

V

V

L

L

A

A

E

E

M

M

V

V

I

N

D

D

A

A

V

V

P

P

S

S

I

I

E

E

M

M

V

V

R

R

F

F

V

V

N

N

S
|
S

G
|
G

T
|
T

E

E

A

A

C

C

M

M

S

A

V

V

L

L

R

R

L

I

M

M

R

R

A

A

F

Y

T

T

G

G

R

R

D

D

K

K

I

I

I

I

K

K

F

F

Q

E

G

G

C

C

Y
|
Y

H

H

G

G

H

H

A

-

D

-

M

-

F

-

L

-

V

-

Q

-

A

-

G

-

S

-

G

-

V

-

A

-

T

-

L

-

G

-

L

-

P

-

D

-

S

-

P

-

G

-

V

-

P

-

K

-

T

-

T

-

T

-

A

-

N

N

T

T

L

L

T

T

A

T

P

P

Y

Y

N

N

D

D

L

L

E

E

A

A

V

V

K

K

A

A

L

L

F

F

A

A

E

E

N

N

P

P

D

G

E

E

I

I

A

A

G

G

V

V

I

I

L

L

E
|
E

P

P

V

I

V

V

G

G

N
|
N

S

S

G

G

F

F

V

I

V

V

P

P

D

D

G

A

G

G

F

F

L

L

Q

E

G

G

L

L

R

R

E

E

L

I

T

T

N

L

E

E

Y

H

G

D

A

A

L

L

L

L

V

V

F

F

D
|
D

E

E

V
|
V

M
|
M

T

T

G

G

F

F

R

R

L

I

S

A

Y

Y

G

G

G

G

A

V

Q

Q

E

E

K

K

F

F

G

G

V

V

T

T

P

P

D

D

L

L

T

T

T

T

L

L

G

G

K
|
K

V

I

I

I

G

G

G

G

G

G

L

L

P

P

V

V

G

G

A

A

Y

Y

G

G

G

G

R

K

K

R

D

E

I

I

M

M

S

Q

M

L

V

V

A

A

P

P

A

A

G

G

P

P

M

M

Y

Y

Q

Q

A

A

G
|
G

T
|
T

L

L

S

S

G

G

N

N

P

P

L

L

A

A

M

M

T

T

A

A

G

G

I

I

K

K

T

T

L

L

E

E

L

L

L

L

Q

R

K

Q

P

P

G

G

T

T

Y

Y

N

E

Q

Y

L

L

E

D

Q

Q

I

I

T

T

Q

K

Q

R

L

L

S

S

E

D

G

G

L

L

L

L

K

A

I

I

A

A

K

Q

E

E

A

T

G

G

H

H

Q

A

V

A

C

C

G

G

G

G

Y

Q

I

V

G

S

A

G

M

M

F

F

G

G

L

F

F

F

F

F

T

T

E

E

G

G

P

P

V

V

R

H

N

N

Y

Y

D

E

D

D

A

A

K

K

K

K

S

S

D

D

L

L

A

Q

K

K

F

F

G

S

R

R

F

F

H

H

R

R

G

G

M

M

L

L

E

E

K

Q

G

G

V

I

Y

Y

L

L

A

A

P

P

S

S

Q

Q

F

F

E

E

A
|
A

G

G

F

F

T

T

S

S

L

L

A

A

H

H

T

T

S

E

E

E

D

D

I

I

E

D

K

A

T

T

L

L

A

A

A

A

A

A

K

R

D

T

V

V

L

M

S

S

N

A

L

L

active site: V21 (= V27), Y144 (= Y150), E177 (= E212), D210 (= D245), M213 (= M248), K238 (= K273), A372 (= A407)
binding (4s)-4-amino-5-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]pentanoic acid: G269 (= G304), T270 (= T305)
binding (4s)-4,5-diaminopentanoic acid: S23 (= S29), V25 (= V31), R26 (= R32), W61 (= W67), Y144 (= Y150)
binding pyridoxal-5'-phosphate: S116 (= S122), G117 (= G123), T118 (= T124), Y144 (= Y150), E177 (= E212), N182 (= N217), D210 (= D245), V212 (= V247), M213 (= M248), K238 (= K273)

Q42522 Glutamate-1-semialdehyde 2,1-aminomutase 2, chloroplastic; GSA 2; Glutamate-1-semialdehyde aminotransferase 2; GSA-AT 2; EC 5.4.3.8 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
73% identity, 99% coverage: 4:433/433 of query aligns to 43:472/472 of Q42522

query
sites
Q42522

T

T

T

K

S

K

L

F

K

T

T

L

T

Q

K

K

S

S

E

E

D

E

I

A

F

F

A

N

A

A

A

A

Q

K

K

N

L

L

M

M

P

P

G

G

G

G

V

V

S

N

S

S

P

P

V

V

R

R

A

A

F

F

K

K

S

S

V

V

G

G

G

G

Q

Q

P

P

I

V

V

V

F

M

D

D

H

S

V

A

K

K

G

G

A

S

Y

R

I

I

W
x
R

D

D

V

I

D

D

G

G

N

N

Q

E

Y

Y

I

I

D

D

Y

Y

V

V

G

G

T

S

W

W

G

G

P

P

A

A

I

I

C

I

G

G

H

H

A

A

H

D

P

D

E

E

V

V

I

L

A

A

A

A

L

L

H

A

D

E

A

T

L

M

D

K

R

K

G

G

T

T

S

S

F

F

G

G

A

A

P

P

C

C

L

L

L

L

E

E

N

N

V

V

L

L

A

A

E

E

M

M

V

V

I

I

D

S

A

A

V

V

P

P

S

S

I

I

E

E

M

M

V

V</