SitesBLAST

N7 (= N7) mutation to A: Almost abolishes catalytic activity. Requires increased magnesium levels for activity. Strongly decreases affinity for acetate.; mutation to D: Almost abolishes catalytic activity. Strongly decreases affinity for acetate.
S10 (= S10) mutation S->A,T: Strongly decreases catalytic activity. Strongly decreases affinity for acetate.
S12 (= S12) mutation to A: Decreases catalytic activity. Strongly decreases affinity for acetate. Requires increased magnesium levels for enzyme activity.; mutation to T: Decreases catalytic activity. Strongly decreases affinity for acetate.
K14 (= K14) mutation to A: Strongly decreases enzyme activity.; mutation to R: Reduces enzyme activity.
R91 (= R98) mutation R->A,L: Decreases catalytic activity. Decreases affinity for acetate.
V93 (= V100) mutation to A: Decreases affinity for acetate.
L122 (≠ T129) mutation to A: Decreases affinity for acetate.
D148 (= D155) active site, Proton donor/acceptor; mutation D->A,E,N: Abolishes catalytic activity. Decreases affinity for acetate, but not for ATP.
F179 (= F185) mutation to A: Decreases affinity for acetate.
N211 (= N217) mutation to A: Slightly reduced enzyme activity.
P232 (= P238) mutation to A: Decreases affinity for acetate.
R241 (= R247) mutation R->K,L: Decreases catalytic activity. Strongly reduced affinity for ATP.
E384 (= E390) mutation to A: Almost abolishes catalytic activity. Strongly decreases affinity for acetate. Requires strongly increased magnesium levels for enzyme activity.

1tuuA Acetate kinase crystallized with atpgs (see paper)
39% identity, 99% coverage: 1:404/407 of query aligns to 1:398/399 of 1tuuA

query
sites
1tuuA

M

M

N

K

I

V

L

L

V

V

L

I

N
|
N

A

A

G

G

S

S

Q

L

K
|
K

S

Y

C

Q

L

L

Y

-

C

-

L

-

D

-

E

-

K

-

E

-

L

I

P

D

Q

M

E

T

T

N

Q

E

K

S

P

A

I

L

W

A

S

V

A

G

N

L

I

C

D

E

W

R

T

I

A

G

T

I

S

D

N

N

Q

S

G

I

V

I

F

T

N

Q

V

K

K

K

A

F

N

D

G

G

I

K

K

K

Q

L

H

E

L

K

T

L

L

T

E

D

S

L

N

P

D

T

H

H

H

K

Q

D

G

A

I

L

R

E

Q

E

M

V

L

V

D

K

T

A

L

L

I

T

Q

D

G

D

K

E

T

F

K

G

V

V

I

I

D

K

N

D

F

M

S

G

A

E

I

I

N

N

L

A

V

V

G

G

H

H

R
|
R

V

V

H

H

G

G

T

E

N

K

Y

F

S

T

E

T

A

S

T

A

L

L

I

Y

N

D

S

E

D

G

V

V

K

E

A

K

T

A

I

I

T

K

E

D

L

C

I

F

P

E

L

L

A

A

P

P

T

L

H

H

N

N

P

P

A

P

H

N

L

M

E

M

G

G

I

I

E

S

I

A

I

C

E

A

S

E

I

I

L

M

N

P

N

G

I

T

P

P

Q

M

V

V

A

I

V

V

F

F

D

D

T

T

A

A

F

F

H

H

S

Q

Q

T

M

M

P

P

L

P

E

Y

N

A

A

Y

A

M

Y

Y

P

A

I

L

P

P

Y

Y

Q

D

W

L

L

Y

E

E

K

K

-

H

G

G

I

V

R

R

R

K

Y

Y

G

G

F

F

H
|
H

G

G

I

T

S

S

H

H

Q

K

Y

Y

C

V

A

A

N

E

R

R

V

A

A

A

E

L

L

M

M

L

N

G

Q

K

P

P

L

A

S

E

L

T

K

K

L

I

I

I

T

T

C

C

H

H

L

L

G
|
G

N

N

G
|
G

C

S

S

S

L

I

A

T

A

A

I

V

K

E

N

G

G

G

I

K

S

S

I

V

D

E

T

T

T

S

M

M

G

G

F

F

T

T

P

P

L

L

E

E

G

G

L

L

M

A

M

M

G

G

T

T

R
|
R

G

C

G

G

S

S

I

I

D

D

P

P

A

A

I

I

L

V

I

P

Y

F

L

L

M

M

R

E

E

K

H

E

N

G

F

L

Q

T

A

T

E

R

E

E

L

I

N

D

H

T

L

L

L

M

N

N

Q

K

E

K

S

S

G

G

L

V

K

L

G

G

I

V

S

S

G

G

I

L

S

S

S

N

D
|
D

M
x
F

R
|
R

A

D

I

L

C

D

Q

E

G

A

I

A

K

S

E

K

G

G

N

N

Q

R

R

K

A

A

K

E

L

L

A

A

F

L

D

E

M

I

F

F

I

A

H

Y

R

K

L

V

H

K

S

K

C

F

L

I

G

G

S

E

M

Y

L

S

T

A

S

V

L

L

G

N

G

G

L

A

D

D

A

A

L

V

V

V

F

F

T

T

A

A

G
|
G

I
|
I

G

G

E

E

N
|
N

S

S

T

A

I

S

V

I

R

R

E

K

K

R

A

I

C

L

E

T

A

G

L

L

S

D

F

G

L

I

G

G

L

I

E

K

L

I

D

D

L

D

F

E

K

K

N

N

N

K

D

I

S

R

P

G

V

Q

G

E

V

I

D

D

I

I

S

S

S

T

S

P

D

D

S

A

S

K

I

V

K

R

V

V

F

F

V

V

I

I

Q

P

T

T

Q

N

E
|
E

D

E

W

L

A

A

I

I

A

A

Q

R

S

E

S

T

W

K

Q

E

L

I

Y

V

N

E

S

T

active site: N7 (= N7), R91 (= R98), H180 (= H186), R241 (= R247), E384 (= E390)
binding adenosine-5'-diphosphate: K14 (= K14), G210 (= G216), D283 (= D289), F284 (≠ M290), R285 (= R291), G331 (= G337), I332 (= I338), N335 (= N341)
binding sulfate ion: R91 (= R98), H180 (= H186), G212 (= G218)

1tuuB Acetate kinase crystallized with atpgs (see paper)
39% identity, 99% coverage: 1:404/407 of query aligns to 1:398/398 of 1tuuB

query
sites
1tuuB

M

M

N

K

I

V

L

L

V

V

L

I

N
|
N

A

A

G

G

S

S

Q

L

K

K

S

Y

C

Q

L

L

Y

-

C

-

L

-

D

-

E

-

K

-

E

-

L

I

P

D

Q

M

E

T

T

N

Q

E

K

S

P

A

I

L

W

A

S

V

A

G

N

L

I

C

D

E

W

R

T

I

A

G

T

I

S

D

N

N

Q

S

G

I

V

I

F

T

N

Q

V

K

K

K

A

F

N

D

G

G

I

K

K

K

Q

L

H

E

L

K

T

L

L

T

E

D

S

L

N

P

D

T

H

H

H

K

Q

D

G

A

I

L

R

E

Q

E

M

V

L

V

D

K

T

A

L

L

I

T

Q

D

G

D

K

E

T

F

K

G

V

V

I

I

D

K

N

D

F

M

S

G

A

E

I

I

N

N

L

A

V

V

G

G

H

H

R
|
R

V

V

H

H

G

G

T

E

N

K

Y

F

S

T

E

T

A

S

T

A

L

L

I

Y

N

D

S

E

D

G

V

V

K

E

A

K

T

A

I

I

T

K

E

D

L

C

I

F

P

E

L

L

A

A

P

P

T

L

H

H

N

N

P

P

A

P

H

N

L

M

E

M

G

G

I

I

E

S

I

A

I

C

E

A

S

E

I

I

L

M

N

P

N

G

I

T

P

P

Q

M

V

V

A

I

V

V

F

F

D

D

T

T

A

A

F

F

H

H

S

Q

Q

T

M

M

P

P

L

P

E

Y

N

A

A

Y

A

M

Y

Y

P

A

I

L

P

P

Y

Y

Q

D

W

L

L

Y

E

E

K

K

-

H

G

G

I

V

R

R

R

K

Y

Y

G

G

F

F

H
|
H

G

G

I

T

S

S

H

H

Q

K

Y

Y

C

V

A

A

N

E

R

R

V

A

A

A

E

L

L

M

M

L

N

G

Q

K

P

P

L

A

S

E

L

T

K

K

L

I

I

I

T

T

C

C

H

H

L

L

G

G

N

N

G
|
G

C

S

S

S

L

I

A

T

A

A

I

V

K

E

N

G

G

G

I

K

S

S

I

V

D

E

T

T

T

S

M

M

G

G

F

F

T

T

P

P

L

L

E

E

G

G

L

L

M

A

M

M

G

G

T

T

R
|
R

G

C

G

G

S

S

I

I

D

D

P

P

A

A

I

I

L

V

I

P

Y

F

L

L

M

M

R

E

E

K

H

E

N

G

F

L

Q

T

A

T

E

R

E

E

L

I

N

D

H

T

L

L

L

M

N

N

Q

K

E

K

S

S

G

G

L

V

K

L

G

G

I

V

S

S

G

G

I

L

S

S

S

N

D
|
D

M

F

R
|
R

A

D

I

L

C

D

Q

E

G

A

I

A

K

S

E

K

G

G

N

N

Q

R

R

K

A

A

K

E

L

L

A

A

F

L

D

E

M

I

F

F

I

A

H

Y

R

K

L

V

H

K

S

K

C

F

L

I

G

G

S

E

M

Y

L

S

T

A

S

V

L

L

G

N

G

G

L

A

D

D

A

A

L

V

V

V

F

F

T

T

A

A

G
|
G

I
|
I

G

G

E

E

N
|
N

S
|
S

T

A

I

S

V

I

R

R

E

K

K

R

A

I

C

L

E

T

A

G

L

L

S

D

F

G

L

I

G

G

L

I

E

K

L

I

D

D

L

D

F

E

K

K

N

N

N

K

D

I

S

R

P

G

V

Q

G

E

V

I

D

D

I

I

S

S

S

T

S

P

D

D

S

A

S

K

I

V

K

R

V

V

F

F

V

V

I

I

Q

P

T

T

Q

N

E
|
E

D

E

W

L

A

A

I

I

A

A

Q

R

S

E

S

T

W

K

Q

E

L

I

Y

V

N

E

S

T

active site: N7 (= N7), R91 (= R98), H180 (= H186), R241 (= R247), E384 (= E390)
binding adenosine monophosphate: D283 (= D289), R285 (= R291), G331 (= G337), I332 (= I338), N335 (= N341), S336 (= S342)
binding trihydrogen thiodiphosphate: H180 (= H186), G212 (= G218), R241 (= R247)

9dv9A The amp-bound structure of acka from treponema vincentii (see paper)
38% identity, 99% coverage: 1:401/407 of query aligns to 5:403/453 of 9dv9A

query
sites
9dv9A

M

M

N

V

I

I

L

L

V

T

L

L

N

N

A

C

G

G

S

S

Q

V

K

K

S

Y

C

Q

L

V

Y

Y

C

D

L

W

D

D

E

N

K

H

E

S

L

-

P

-

Q

-

E

-

T

-

Q

-

K

-

P

-

I

V

W

L

S

A

A

S

N

G

I

V

D

V

W

E

T

R

A

V

T

T

S

Q

N

P

Q

G

G

S

V

V

F

I

N

T

V

H

K

E

A

A

N

K

G

G

I

-

K

K

Q

D

H

K

L

Y

T

V

L

L

E

E

S

S

N

P

-

C

-

P

D

S

H

H

H

T

Q

H

G

A

I

V

R

E

Q

L

M

I

L

I

D

K

T

T

L

L

I

T

Q

D

G

P

K

S

T

V

K

G

V

V

I

I

D

T

N

D

F

M

S

N

A

V

I

I

N

K

L

A

V

V

G

G

H

H

R

R

V

V

V

T

H

H

G

G

T

D

N

K

Y

F

S

I

E

K

A

S

T

V

L

I

I

V

N

T

S

P

D

E

V

I

K

L

A

N

T

T

I

F

T

R

E

E

L

V

I

Q

P

D

L

L

A

G

P

P

T

L

H

H

N

N

P

P

A

A

H

N

L

I

E

M

G

G

I

I

E

E

I

A

E

Q

S

K

I

V

L

L

N

P

N

N

I

V

P

P

Q

H

V

C

A

A

V

I

F

I

D

D

T

T

A

A

F

W

H

H

S

Q

Q

T

M

M

P

P

L

E

E

T

N

S

A

F

A

M

Y

Y

P

A

I

I

P

P

Y

H

Q

E

W

W

L

Y

E

E

K

K

-

Y

G

S

I

A

R

R

Y

Y

G

G

F

F

H

H

G

G

I

T

S

S

H

F

Q

L

Y

Y

C

T

A

A

N

K

R

R

V

A

A

A

E

V

L

I

M

L

N

G

Q

K

P

K

L

P

S

E

S

D

L

T

K

N

L

I

I

I

T

I

C

A

H

H

L

I

G
|
G

N

N

G

G

C

A

S

S

L

M

A

C

I

V

K

K

N

Q

G

G

I

K

S

C

I

F

D

D

T

T

T

S

M

M

G

G

F

L

T

T

P

P

L

L

E

E

G

G

L

L

M

V

M

M

G

G

T

T

R

R

G

S

G

G

S

D

I

C

D

D

P

P

A

A

I

L

L

P

I

F

Y

Y

L

I

M

M

R

R

E

K

H

T

N

G

F

M

Q

T

A

P

E

A

E

E

L

M

N

D

H

T

L

A

L

L

N

N

Q

K

E

K

S

S

G

G

L

L

K

L

G

G

I

V

S

T

G

G

I

Q

S

Y

S

V

D
|
D

M
x
R

R
|
R

A

D

I

V

C

S

Q

K

G

A

I

M

K

G

E

E

G

G

N

D

Q

K

R

R

A

A

K

R

L

L

A

A

F

F

D

N

M

M

F

E

I

V

H

Y

R

R

L

L

H

Q

S

K

C

Y

L

F

G

G

S

A

M

Y

L

I

T

A

S

A

L

L

G

G

-

Q

G

K

L

P

D

D

A

A

L

I

V

V

F

F

T

T

A
|
A

G
|
G

I

V

G

G

E

E

N
x
F

S

G

T

F

I

D

V

T

R

R

E

L

K

A

A

V

C

C

E

E

A

G

L

L

S

T

F

H

L

L

G

G

L

I

E

K

L

I

D

D

L

P

F

K

K

K

N

N

-

A

-

L

-

A

N

R

D

T

S

R

P

N

V

A

G

E

V

T

D

C

I

I

S

S

S

A

S

D

D

D

S

S

S

P

I

V

K

K

V

I

F

F

V

V

I

I

Q

P

T

T

Q

D

E

E

D

E

W

L

A

V

I

M

A

T

Q

E

S

D

S

A

W

Y

Q

A

L

L

binding adenosine monophosphate: G214 (= G216), D287 (= D289), R288 (≠ M290), R289 (= R291), A335 (= A336), G336 (= G337), F340 (≠ N341)

4ijnA Crystal structure of an acetate kinase from mycobacterium smegmatis bound to amp and sulfate (see paper)
41% identity, 82% coverage: 66:397/407 of query aligns to 42:372/376 of 4ijnA

query
sites
4ijnA

D

D

H

H

H

D

Q

A

G

A

I

L

R

R

Q

A

M

A

L

F

D

D

T

E

L

L

I

A

Q

A

G

A

K

G

T

L

K

H

V

L

I

E

D

D

N

-

F

-

S

L

A

D

I

L

N

K

L

A

V

V

G

G

H

H

R
|
R

V

M

V

V

H

H

G

G

T

K

N

T

Y

F

S

Y

E

K

A

P

T

S

L

V

I

V

N

D

S

D

D

E

V

L

K

I

A

A

T

K

I

A

T

R

E

E

L

L

I

S

P

P

L

L

A

A

P

P

T

L

H

H

N

N

P

P

A

P

H

A

L

I

E

K

G

G

I

I

E

E

I

V

I

A

E

R

S

K

I

L

L

L

N

P

N

D

I

L

P

P

Q

H

V

I

A

A

V

V

F

F

D

D

T

T

A

A

F

F

H

F

S

H

Q

D

M

L

P

P

L

A

E

P

N

A

A

S

A

T

Y

Y

P

A

I

I

P

D

Y

R

Q

E

W

L

L

A

E

E

K

T

-

W

G

H

I

I

R

K

R

R

Y

Y

G

G

F

F

H
|
H

G

G

I

T

S

S

H

H

Q

E

Y

Y

C

V

A

S

N

Q

R

Q

V

A

A

A

E

I

L

F

M

L

N

D

Q

R

P

P

L

L

S

E

S

S

L

L

K

N

L

Q

I

I

T

V

C

L

H

H

L

L

G
|
G

N
|
N

G

G

C

A

S

S

L

A

A

S

A

A

I

V

K

A

N

G

G

G

I

K

S

A

I

V

D

D

T

T

T

S

M

M

G

G

F

L

T

T

P

P

L

M

E

E

G

G

L

L

M

V

M

M

G

G

T

T

R
|
R

G

S

G

G

S

D

I

I

D

D

P

P

A

G

I

V

L

I

I

M

Y

Y

L

L

M

W

R

R

E

T

H

A

N

G

F

M

Q

S

A

V

E

D

L

I

N

E

H

S

L

M

L

L

N

N

Q

R

E

R

S

S

G

G

L

V

K

L

G

G

I

L

S

G

G

G

I

-

S

A

S

S

D
|
D

M
x
F

R
|
R

A

K

I

L

C

R

Q

E

G

L

I

I

K

E

E

S

G

G

N

D

Q

E

R

H

A

A

K

K

L

L

A

A

F

Y

D

D

M

V

F

Y

I

I

H

H

R

R

L

L

H

R

S

K

C

Y

L

I

G

G

S

A

M

Y

L

M

T

A

S

V

L

L

G

G

G

R

L

T

D

D

A

V

L

I

V

S

F

F

T

T

A

A

G
|
G

I
x
V

G

G

E

E

N
|
N

S
x
V

T

P

I

P

V

V

R

R

E

R

K

D

A

A

C

L

E

A

A

G

L

L

S

G

F

G

L

L

G

G

L

I

E

E

L

I

D

D

L

D

F

A

K

L

N

N

-

S

-

A

-

K

N

S

D

D

S

E

P

P

V

-

G

-

V

R

D

L

I

I

S

S

S

T

S

P

D

D

S

S

S

R

I

V

K

T

V

V

F

L

V

V

I

V

Q

P

T

T

Q

N

E
|
E

D

E

W

L

A

A

I

I

A

A

Q

R

S

A

active site: R72 (= R98), H161 (= H186), R222 (= R247), E365 (= E390)
binding adenosine monophosphate: G191 (= G216), N192 (= N217), D263 (= D289), F264 (≠ M290), R265 (= R291), G311 (= G337), V312 (≠ I338), N315 (= N341), V316 (≠ S342)

Sites not aligning to the query:

active site: 8

4iz9A Crystal structure of an acetate kinase from mycobacterium avium bound to an unknown acid-apcpp conjugate and manganese (see paper)
42% identity, 83% coverage: 66:401/407 of query aligns to 44:378/381 of 4iz9A

query
sites
4iz9A

D

D

H

H

H

D

Q

A

G

A

I

L

R

R

Q

R

M

A

L

F

D

D

T

M

L

L

I

A

Q

G

G

D

K

G

T

V

K

D

V

L

I

-

D

-

N

N

F

T

S

A

A

G

I

L

N

V

L

A

V

V

G

G

H

H

R
|
R

V

V

H

H

G

G

T

N

N

T

Y

F

S

Y

E

R

A

P

T

T

L

V

I

L

N

D

S

D

D

A

V

V

K

I

A

A

T

R

I

L

T

H

E

E

L

L

I

S

P

E

L

L

A

A

P

P

T

L

H

H

N

N

P

P

A

P

H

A

L

L

E

L

G

G

I

I

E

E

I

V

I

A

E

R

S

R

I

L

L

L

N

P

N

G

I

I

P

A

Q

H

V

V

A

A

V

V

F

F

D

D

T

T

A

G

F

F

H

F

S

H

Q

D

M

L

P

P

L

P

E

A

N

A

A

A

A

T

Y

Y

P

A

I

I

P

D

Y

R

Q

E

W

L

L

A

E

D

K

R

-

W

G

Q

I

I

R

R

Y

Y

G

G

F

F

H
|
H

G

G

I

T

S

S

H

H

Q

R

Y

Y

C

V

A

S

N

E

R

Q

V

A

A

A

E

A

L

F

M

L

N

D

Q

R

P

P

L

L

S

R

S

G

L

L

K

K

L

Q

I

I

T

V

C

L

H

H

L

L

G
|
G

N
|
N

G

G

C

C

S

S

L

A

A

S

A

A

I

I

K

A

N

G

G

T

I

R

S

P

I

L

D

D

T

T

T

S

M

M

G

G

F

L

T

T

P

P

L

L

E

E

G

G

L

L

M

V

M

M

G

G

T

T

R
|
R

G

S

G

G

S

D

I

I

D

D

P

P

A

S

I

V

L

V

I

S

Y

Y

L

L

M

C

R

H

E

T

H

A

N

G

F

M

Q

G

A

V

E

D

L

V

N

E

H

S

L

M

L

L

N

N

Q

H

E

R

S

S

G

G

L

V

K

V

G

G

I

L

S

S

G

G

I

V

S

-

S

R

D
|
D

M
x
F

R
|
R

A

R

I

L

C

R

Q

E

G

L

I

I

K

E

E

S

G

G

N

D

Q

G

R

A

A

A

K

Q

L

L

A

A

F

Y

D

S

M

V

F

F

I

T

H

H

R

R

L

L

H

R

S

K

C

Y

L

I

G

G

S

A

M

Y

L

L

T

A

S

V

L

L

G

G

G

H

L

T

D

D

A

V

L

I

V

S

F

F

T

T

A

A

G
|
G

I
|
I

G

G

E

E

N
|
N

S
x
D

T

A

I

A

V

V

R

R

E

R

K

D

A

A

C

V

E

S

A

G

L

M

S

E

F

E

L

L

G

G

L

I

E

V

L

L

D

D

L

E

F

R

K

R

N

N

N

L

D

P

S

G

P

A

V

K

G

G

V

A

-

R

D

Q

I

I

S

S

S

A

S

D

D

D

S

S

S

P

I

I

K

T

V

V

F

L

V

V

I

V

Q

P

T

T

Q

N

E
|
E

D

E

W

L

A

A

I

I

A

A

Q

R

S

D

S

C

W

V

Q

R

L

V

active site: R74 (= R98), H163 (= H186), R224 (= R247), E367 (= E390)
binding diphosphomethylphosphonic acid adenosyl ester: G193 (= G216), N194 (= N217), D265 (= D289), F266 (≠ M290), R267 (= R291), G313 (= G337), I314 (= I338), N317 (= N341), D318 (≠ S342)

Sites not aligning to the query:

active site: 10
binding diphosphomethylphosphonic acid adenosyl ester: 17

4fwsA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with ctp (see paper)
41% identity, 78% coverage: 87:403/407 of query aligns to 72:391/394 of 4fwsA

query
sites
4fwsA

D

D

N

L

F

T

S

D

A

S

I

V

N

A

L

L

V

I

G

G

H

H

R
|
R

V

I

V

A

H

H

G

G

T

E

N

L

Y

F

S

T

E

Q

A

S

T

V

L

I

I

I

N

T

S

D

D

E

V

I

K

I

A

D

T

N

I

I

T

R

E

R

L

V

I

S

P

P

L

L

A

A

P

P

T

L

H
|
H

N

N

P

Y

A

A

H

N

L

L

E

S

G

G

I

I

E

D

I

A

E

R

S

H

I

L

L

F

N

P

N

A

I

V

P

R

Q

Q

V

V

A

A

V

V

F

F

D

D

T

T

A

S

F

F

H

H

S

Q

Q

T

M

L

P

A

L

P

E

E

N

A

A

Y

A

L

Y

Y

P

G

I

L

P

P

Y

W

Q

E

W

Y

L

F

E

S

K

S

-

L

G

G

I

V

R

R

Y

Y

G

G

F

F

H
|
H

G

G

I

T

S

S

H

H

Q

R

Y

Y

C

V

A

S

N

R

R

R

V

A

A

Y

E

E

L

L

M

L

N

D

Q

L

P

D

L

E

S

K

S

D

L

S

K

G

L

L

I

I

T

V

C

A

H

H

L

L

G
|
G

N
|
N

G
|
G

C

A

S

S

L

I

A

C

A

A

I

V

K

R

N

N

G

G

I

Q

S

S

I

V

D

D

T

T

T

S

M

M

G

G

F

M

T

T

P

P

L

L

E

E

G

G

L

L

M

M

G

G

T
|
T

R
|
R

G

S

G

G

S

D

I

V

D

D

P

F

A

G

I

A

L

M

I

A

Y

W

L

I

M

A

R

K

E

E

H

T

N

G

F

Q

Q

T

A

L

E

S

E

D

L

L

N

E

H

R

L

V

N

N

Q
x
K

E

E

S

S

G

G

L

L

K

L

G

G

I

I

S

S

G

G

I

L

S

S

D
|
D

M
x
L

R
|
R

A

V

I

L

C

E

Q

K

G

A

I

W

K

H

E

E

G

G

N

H

Q

E

R

R

A

A

K

R

L

L

A

A

F

I

D

K

M

T

F

F

I

V

H

H

R

R

L

I

H

A

S

R

C

H

L

I

G

A

S

G

M

H

L

A

T

A

S

S

L

L

G

H

G

R

L

L

D

D

A

G

L

I

V

I

F

F

T

T

A

G

G
|
G

I
|
I

G

G

E

E

N
|
N

S

S

T

V

I

L

V

I

R

R

E

Q

K

L

A

V

C

I

E

E

A

H

L

L

S

G

F

V

L

L

G

G

L

L

E

T

L

L

D

D

L

V

F

E

K

M

N

N

N

K

-

Q

-

P

D

N

S

S

P

H

V

G

G

E

V

R

D

I

I

I

S

S

S

A

S

N

D

P

S

S

S

Q

I

V

K

I

V

C

F

A

V

V

I

I

Q

P

T

T

Q

N

E
|
E

D

E

W

K

A

M

I

I

A

A

Q

L

S

D

S

A

W

I

Q

H

L

L

Y

G

N

N

active site: R83 (= R98), H172 (= H186), R233 (= R247), E378 (= E390)
binding cytidine-5'-triphosphate: G202 (= G216), N203 (= N217), G204 (= G218), D275 (= D289), L276 (≠ M290), R277 (= R291), G323 (= G337), I324 (= I338), N327 (= N341)
binding 1,2-ethanediol: H115 (= H130), N203 (= N217), T232 (= T246), R233 (= R247), K262 (≠ Q276)

Sites not aligning to the query:

active site: 8
binding 1,2-ethanediol: 21, 24

4fwrA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with cmp (see paper)
41% identity, 78% coverage: 87:403/407 of query aligns to 72:391/394 of 4fwrA

query
sites
4fwrA

D

D

N

L

F

T

S

D

A

S

I

V

N

A

L

L

V

I

G

G

H

H

R
|
R

V

I

V

A

H

H

G

G

T

E

N

L

Y

F

S

T

E

Q

A

S

T

V

L

I

I

I

N

T

S

D

D

E

V

I

K

I

A

D

T

N

I

I

T

R

E

R

L

V

I

S

P

P

L

L

A

A

P

P

T

L

H

H

N

N

P

Y

A

A

H

N

L

L

E

S

G

G

I

I

E

D

I

A

E

R

S

H

I

L

L

F

N

P

N

A

I

V

P

R

Q

Q

V

V

A

A

V

V

F

F

D

D

T

T

A

S

F

F

H

H

S

Q

Q

T

M

L

P

A

L

P

E

E

N

A

A

Y

A

L

Y

Y

P

G

I

L

P

P

Y

W

Q

E

W

Y

L

F

E

S

K

S

-

L

G

G

I

V

R

R

Y

Y

G

G

F

F

H
|
H

G

G

I

T

S

S

H

H

Q

R

Y

Y

C

V

A

S

N

R

R

R

V

A

A

Y

E

E

L

L

M

L

N

D

Q

L

P

D

L

E

S

K

S

D

L

S

K

G

L

L

I

I

T

V

C

A

H

H

L

L

G
|
G

N
|
N

G

G

C

A

S

S

L

I

A

C

A

A

I

V

K

R

N

N

G

G

I

Q

S

S

I

V

D

D

T

T

T

S

M

M

G

G

F

M

T

T

P

P

L

L

E

E

G

G

L

L

M

M

G

G

T

T

R
|
R

G

S

G

G

S

D

I

V

D

D

P

F

A

G

I

A

L

M

I

A

Y

W

L

I

M

A

R

K

E

E

H

T

N

G

F

Q

Q

T

A

L

E

S

E

D

L

L

N

E

H

R

L

V

N

N

Q

K

E

E

S

S

G

G

L

L

K

L

G

G

I

I

S

S

G

G

I

L

S

S

D
|
D

M
x
L

R
|
R

A

V

I

L

C

E

Q

K

G

A

I

W

K

H

E

E

G

G

N

H

Q

E

R

R

A

A

K

R

L

L

A

A

F

I

D

K

M

T

F

F

I

V

H

H

R

R

L

I

H

A

S

R

C

H

L

I

G

A

S

G

M

H

L

A

T

A

S

S

L

L

G

H

G

R

L

L

D

D

A

G

L

I

V

I

F

F

T

T

A

G

G
|
G

I
|
I

G

G

E

E

N
|
N

S

S

T

V

I

L

V

I

R

R

E

Q

K

L

A

V

C

I

E

E

A

H

L

L

S

G

F

V

L

L

G

G

L

L

E

T

L

L

D

D

L

V

F

E

K

M

N

N

N

K

-

Q

-

P

D

N

S

S

P

H

V

G

G

E

V

R

D

I

I

I

S

S

S

A

S

N

D

P

S

S

S

Q

I

V

K

I

V

C

F

A

V

V

I

I

Q

P

T

T

Q

N

E
|
E

D

E

W

K

A

M

I

I

A

A

Q

L

S

D

S

A

W

I

Q

H

L

L

Y

G

N

N

active site: R83 (= R98), H172 (= H186), R233 (= R247), E378 (= E390)
binding cytidine-5'-monophosphate: G202 (= G216), N203 (= N217), D275 (= D289), L276 (≠ M290), R277 (= R291), G323 (= G337), I324 (= I338), N327 (= N341)

Sites not aligning to the query:

active site: 8

4fwqA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with gtp (see paper)
41% identity, 78% coverage: 87:403/407 of query aligns to 72:391/394 of 4fwqA

query
sites
4fwqA

D

D

N

L

F

T

S

D

A

S

I

V

N

A

L

L

V

I

G

G

H

H

R
|
R

V

I

V

A

H

H

G

G

T

E

N

L

Y

F

S

T

E

Q

A

S

T

V

L

I

I

I

N

T

S

D

D

E

V

I

K

I

A

D

T

N

I

I

T

R

E

R

L

V

I

S

P

P

L

L

A

A

P

P

T

L

H

H

N

N

P

Y

A

A

H

N

L

L

E

S

G

G

I

I

E

D

I

A

E

R

S

H

I

L

L

F

N

P

N

A

I

V

P

R

Q

Q

V

V

A

A

V

V

F

F

D

D

T

T

A

S

F

F

H

H

S

Q

Q

T

M

L

P

A

L

P

E

E

N

A

A

Y

A

L

Y

Y

P

G

I

L

P

P

Y

W

Q

E

W

Y

L

F

E

S

K

S

-

L

G

G

I

V

R

R

Y

Y

G

G

F

F

H
|
H

G

G

I

T

S

S

H

H

Q

R

Y

Y

C

V

A

S

N

R

R

R

V

A

A

Y

E

E

L

L

M

L

N

D

Q

L

P

D

L

E

S

K

S

D

L

S

K

G

L

L

I

I

T

V

C

A

H

H

L

L

G

G

N
|
N

G
|
G

C

A

S

S

L

I

A

C

A

A

I

V

K

R

N

N

G

G

I

Q

S

S

I

V

D

D

T

T

T

S

M

M

G

G

F

M

T

T

P

P

L

L

E

E

G

G

L

L

M

M

G

G

T

T

R
|
R

G

S

G

G

S

D

I

V

D

D

P

F

A

G

I

A

L

M

I

A

Y

W

L

I

M

A

R

K

E

E

H

T

N

G

F

Q

Q

T

A

L

E

S

E

D

L

L

N

E

H

R

L

V

N

N

Q

K

E

E

S

S

G

G

L

L

K

L

G

G

I

I

S

S

G

G

I

L

S

S

D
|
D

M
x
L

R
|
R

A

V

I

L

C
x
E

Q

K

G

A

I

W

K

H

E

E

G

G

N

H

Q

E

R

R

A

A

K

R

L

L

A

A

F

I

D

K

M

T

F

F

I

V

H

H

R

R

L

I

H

A

S

R

C

H

L

I

G

A

S

G

M

H

L

A

T

A

S

S

L

L

G

H

G

R

L

L

D

D

A

G

L

I

V

I

F

F

T

T

A

G

G
|
G

I
|
I

G

G

E

E

N
|
N

S

S

T

V

I

L

V

I

R

R

E

Q

K

L

A

V

C

I

E

E

A

H

L

L

S

G

F

V

L

L

G

G

L

L

E

T

L

L

D

D

L

V

F

E

K

M

N

N

N

K

-

Q

-

P

D

N

S

S

P

H

V

G

G

E

V

R

D

I

I

I

S

S

S

A

S

N

D

P

S

S

S

Q

I

V

K

I

V

C

F

A

V

V

I

I

Q

P

T

T

Q

N

E
|
E

D

E

W

K

A

M

I

I

A

A

Q

L

S

D

S

A

W

I

Q

H

L

L

Y

G

N

N

active site: R83 (= R98), H172 (= H186), R233 (= R247), E378 (= E390)
binding guanosine-5'-triphosphate: H172 (= H186), N203 (= N217), G204 (= G218), D275 (= D289), L276 (≠ M290), R277 (= R291), E280 (≠ C294), G323 (= G337), I324 (= I338), N327 (= N341)

Sites not aligning to the query:

active site: 8

4fwpA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with gdp (see paper)
41% identity, 78% coverage: 87:403/407 of query aligns to 72:391/394 of 4fwpA

query
sites
4fwpA

D

D

N

L

F

T

S

D

A

S

I

V

N

A

L

L

V

I

G

G

H

H

R
|
R

V

I

V

A

H

H

G

G

T

E

N

L

Y

F

S

T

E

Q

A

S

T

V

L

I

I

I

N

T

S

D

D

E

V

I

K

I

A

D

T

N

I

I

T

R

E

R

L

V

I

S

P

P

L

L

A

A

P

P

T

L

H
|
H

N

N

P

Y

A

A

H

N

L

L

E

S

G

G

I

I

E

D

I

A

E

R

S

H

I

L

L

F

N

P

N

A

I

V

P

R

Q

Q

V

V

A

A

V

V

F

F

D

D

T

T

A

S

F

F

H

H

S

Q

Q

T

M

L

P

A

L

P

E

E

N

A

A

Y

A

L

Y

Y

P

G

I

L

P

P

Y

W

Q

E

W

Y

L

F

E

S

K

S

-

L

G

G

I

V

R

R

Y

Y

G

G

F

F

H
|
H

G

G

I

T

S

S

H

H

Q

R

Y

Y

C

V

A

S

N

R

R

R

V

A

A

Y

E

E

L

L

M

L

N

D

Q

L

P

D

L

E

S

K

S

D

L

S

K

G

L

L

I

I

T

V

C

A

H

H

L

L

G

G

N
|
N

G

G

C

A

S

S

L

I

A

C

A

A

I

V

K

R

N

N

G

G

I

Q

S

S

I

V

D

D

T

T

T

S

M

M

G

G

F

M

T

T

P

P

L

L

E

E

G

G

L

L

M

M

G

G

T

T

R
|
R

G

S

G

G

S

D

I

V

D

D

P

F

A

G

I

A

L

M

I

A

Y

W

L

I

M

A

R

K

E

E

H

T

N

G

F

Q

Q

T

A

L

E

S

E

D

L

L

N

E

H

R

L

V

N

N

Q
x
K

E

E

S

S

G

G

L

L

K

L

G

G

I

I

S

S

G

G

I

L

S

S

D
|
D

M
x
L

R
|
R

A

V

I

L

C
x
E

Q

K

G

A

I

W

K

H

E

E

G

G

N

H

Q

E

R

R

A

A

K

R

L

L

A

A

F

I

D

K

M

T

F

F

I

V

H

H

R

R

L

I

H

A

S

R

C

H

L

I

G

A

S

G

M

H

L

A

T

A

S

S

L

L

G

H

G

R

L

L

D

D

A

G

L

I

V

I

F

F

T

T

A

G

G
|
G

I
|
I

G

G

E

E

N
|
N

S
|
S

T

V

I

L

V

I

R

R

E

Q

K

L

A

V

C

I

E

E

A

H

L

L

S

G

F

V

L

L

G

G

L

L

E

T

L

L

D

D

L

V

F

E

K

M

N

N

N

K

-

Q

-

P

D

N

S

S

P

H

V

G

G

E

V

R

D

I

I

I

S

S

S

A

S

N

D

P

S

S

S

Q

I

V

K

I

V

C

F

A

V

V

I

I

Q

P

T

T

Q

N

E
|
E

D

E

W

K

A

M

I

I

A

A

Q

L

S

D

S

A

W

I

Q

H

L

L

Y

G

N

N

active site: R83 (= R98), H172 (= H186), R233 (= R247), E378 (= E390)
binding 1,2-ethanediol: H115 (= H130), K262 (≠ Q276)
binding guanosine-5'-diphosphate: N203 (= N217), D275 (= D289), L276 (≠ M290), R277 (= R291), E280 (≠ C294), G323 (= G337), I324 (= I338), N327 (= N341), S328 (= S342)

Sites not aligning to the query:

active site: 8
binding 1,2-ethanediol: 11

4fwoA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with gmp (see paper)
41% identity, 78% coverage: 87:403/407 of query aligns to 72:391/394 of 4fwoA

query
sites
4fwoA

D

D

N

L

F

T

S

D

A

S

I

V

N

A

L

L

V

I

G

G

H

H

R
|
R

V

I

V

A

H

H

G

G

T

E

N

L

Y

F

S

T

E

Q

A

S

T

V

L

I

I

I

N

T

S

D

D
x
E

V

I

K

I

A

D

T
x
N

I

I

T

R

E

R

L

V

I

S

P

P

L

L

A

A

P

P

T

L

H

H

N

N

P

Y

A

A

H

N

L

L

E

S

G

G

I

I

E

D

I

A

E

R

S

H

I

L

L

F

N

P

N

A

I

V

P

R

Q

Q

V

V

A

A

V

V

F

F

D

D

T

T

A

S

F

F

H

H

S

Q

Q

T

M

L

P

A

L

P

E

E

N

A

A

Y

A

L

Y

Y

P

G

I

L

P

P

Y

W

Q

E

W

Y

L

F

E

S

K

S

-

L

G

G

I

V

R

R

Y

Y

G

G

F

F

H
|
H

G

G

I

T

S

S

H

H

Q

R

Y

Y

C

V

A

S

N

R

R

R

V

A

A

Y

E

E

L

L

M

L

N

D

Q

L

P

D

L

E

S

K

S

D

L

S

K

G

L

L

I

I

T

V

C

A

H

H

L

L

G
|
G

N
|
N

G

G

C

A

S

S

L

I

A

C

A

A

I

V

K

R

N

N

G

G

I

Q

S

S

I

V

D

D

T

T

T

S

M

M

G

G

F

M

T

T

P

P

L

L

E

E

G

G

L

L

M

M

G

G

T

T

R
|
R

G

S

G

G

S

D

I

V

D

D

P

F

A

G

I

A

L

M

I

A

Y

W

L

I

M

A

R

K

E

E

H

T

N

G

F

Q

Q

T

A

L

E

S

E

D

L

L

N

E

H

R

L

V

N

N

Q

K

E

E

S

S

G

G

L

L

K

L

G

G

I

I

S

S

G

G

I

L

S

S

D
|
D

M
x
L

R
|
R

A

V

I

L

C
x
E

Q

K

G

A

I

W

K

H

E

E

G

G

N

H

Q

E

R

R

A

A

K

R

L

L

A

A

F

I

D

K

M

T

F

F

I

V

H

H

R

R

L

I

H

A

S

R

C

H

L

I

G

A

S

G

M

H

L

A

T

A

S

S

L

L

G

H

G

R

L

L

D

D

A

G

L

I

V

I

F

F

T

T

A

G

G
|
G

I
|
I

G

G

E

E

N
|
N

S

S

T

V

I

L

V

I

R

R

E

Q

K

L

A

V

C

I

E

E

A

H

L

L

S

G

F

V

L

L

G

G

L

L

E

T

L

L

D

D

L

V

F

E

K

M

N

N

N

K

-

Q

-

P

D

N

S

S

P

H

V

G

G

E

V

R

D

I

I

I

S

S

S

A

S

N

D

P

S

S

S

Q

I

V

K

I

V

C

F

A

V

V

I

I

Q

P

T

T

Q

N

E
|
E

D

E

W

K

A

M

I

I

A

A

Q

L

S

D

S

A

W

I

Q

H

L

L

Y

G

N

N

active site: R83 (= R98), H172 (= H186), R233 (= R247), E378 (= E390)
binding guanosine-5'-monophosphate: G202 (= G216), N203 (= N217), D275 (= D289), L276 (≠ M290), R277 (= R291), E280 (≠ C294), G323 (= G337), I324 (= I338), N327 (= N341)
binding 1,2-ethanediol: E100 (≠ D115), N104 (≠ T119)

Sites not aligning to the query:

active site: 8

4fwnA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with adenosine tetraphosphate (ap4) (see paper)
41% identity, 78% coverage: 87:403/407 of query aligns to 72:391/394 of 4fwnA

query
sites
4fwnA

D

D

N

L

F

T

S

D

A

S

I

V

N

A

L

L

V

I

G

G

H

H

R
|
R

V

I

V

A

H

H

G

G

T

E

N

L

Y

F

S

T

E

Q

A

S

T

V

L

I

I

I

N

T

S

D

D

E

V

I

K

I

A

D

T

N

I

I

T

R

E

R

L

V

I

S

P

P

L

L

A

A

P

P

T

L

H

H

N

N

P

Y

A

A

H

N

L

L

E

S

G

G

I

I

E

D

I

A

E

R

S

H

I

L

L

F

N

P

N

A

I

V

P

R

Q

Q

V

V

A

A

V

V

F

F

D

D

T

T

A

S

F

F

H

H

S

Q

Q

T

M

L

P

A

L

P

E

E

N

A

A

Y

A

L

Y

Y

P

G

I

L

P

P

Y

W

Q

E

W

Y

L

F

E

S

K

S

-

L

G

G

I

V

R

R

Y

Y

G

G

F

F

H
|
H

G

G

I

T

S

S

H

H

Q

R

Y

Y

C

V

A

S

N

R

R

R

V

A

A

Y

E

E

L

L

M

L

N

D

Q

L

P

D

L

E

S

K

S

D

L

S

K

G

L

L

I

I

T

V

C

A

H
|
H

L

L

G

G

N
|
N

G
|
G

C

A

S

S

L

I

A

C

A

A

I

V

K

R

N

N

G

G

I

Q

S

S

I

V

D

D

T

T

T

S

M

M

G

G

F

M

T

T

P

P

L

L

E

E

G

G

L

L

M

M

G

G

T

T

R
|
R

G

S

G

G

S

D

I

V

D

D

P

F

A

G

I

A

L

M

I

A

Y

W

L

I

M

A

R

K

E

E

H

T

N

G

F

Q

Q

T

A

L

E

S

E

D

L

L

N

E

H

R

L

V

N

N

Q

K

E

E

S

S

G

G

L

L

K

L

G

G

I

I

S

S

G

G

I

L

S

S

D
|
D

M
x
L

R
|
R

A

V

I

L

C

E

Q

K

G

A

I

W

K

H

E

E

G

G

N

H

Q

E

R

R

A

A

K

R

L

L

A

A

F

I

D

K

M

T

F

F

I

V

H

H

R

R

L

I

H

A

S

R

C

H

L

I

G

A

S

G

M

H

L

A

T

A

S

S

L

L

G

H

G

R

L

L

D

D

A

G

L

I

V

I

F

F

T

T

A

G

G
|
G

I
|
I

G

G

E

E

N
|
N

S

S

T

V

I

L

V

I

R

R

E

Q

K

L

A

V

C

I

E

E

A

H

L

L

S

G

F

V

L

L

G

G

L

L

E

T

L

L

D

D

L

V

F

E

K

M

N

N

N

K

-

Q

-

P

D

N

S

S

P

H

V

G

G

E

V

R

D

I

I

I

S

S

S

A

S

N

D

P

S

S

S

Q

I

V

K

I

V

C

F

A

V

V

I

I

Q

P

T

T

Q

N

E
|
E

D

E

W

K

A

M

I

I

A

A

Q

L

S

D

S

A

W

I

Q

H

L

L

Y

G

N

N

active site: R83 (= R98), H172 (= H186), R233 (= R247), E378 (= E390)
binding adenosine-5'-tetraphosphate: H172 (= H186), H200 (= H214), N203 (= N217), G204 (= G218), D275 (= D289), L276 (≠ M290), R277 (= R291), G323 (= G337), I324 (= I338), N327 (= N341)

Sites not aligning to the query:

active site: 8

4fwmA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with atp (see paper)
41% identity, 78% coverage: 87:403/407 of query aligns to 72:391/394 of 4fwmA

query
sites
4fwmA

D

D

N

L

F

T

S

D

A

S

I

V

N

A

L

L

V

I

G

G

H

H

R
|
R

V

I

V

A

H

H

G

G

T

E

N

L

Y

F

S

T

E

Q

A

S

T

V

L

I

I

I

N

T

S

D

D

E

V

I

K

I

A

D

T

N

I

I

T

R

E

R

L

V

I

S

P

P

L

L

A

A

P

P

T

L

H

H

N

N

P

Y

A

A

H

N

L

L

E

S

G

G

I

I

E

D

I

A

E

R

S

H

I

L

L

F

N

P

N

A

I

V

P

R

Q

Q

V

V

A

A

V

V

F

F

D

D

T

T

A

S

F

F

H

H

S

Q

Q

T

M

L

P

A

L

P

E

E

N

A

A

Y

A

L

Y

Y

P

G

I

L

P

P

Y

W

Q

E

W

Y

L

F

E

S

K

S

-

L

G

G

I

V

R

R

Y

Y

G

G

F

F

H
|
H

G

G

I

T

S

S

H

H

Q

R

Y

Y

C

V

A

S

N

R

R

R

V

A

A

Y

E

E

L

L

M

L

N

D

Q

L

P

D

L

E

S

K

S

D

L

S

K

G

L

L

I

I

T

V

C

A

H
|
H

L

L

G

G

N
|
N

G
|
G

C

A

S

S

L

I

A

C

A

A

I

V

K

R

N

N

G

G

I

Q

S

S

I

V

D

D

T

T

T

S

M

M

G

G

F

M

T

T

P

P

L

L

E

E

G

G

L

L

M

M

G

G

T

T

R
|
R

G

S

G

G

S

D

I

V

D

D

P

F

A

G

I

A

L

M

I

A

Y

W

L

I

M

A

R

K

E

E

H

T

N

G

F

Q

Q

T

A

L

E

S

E

D

L

L

N

E

H

R

L

V

N

N

Q

K

E

E

S

S

G

G

L

L

K

L

G

G

I

I

S

S

G

G

I

L

S

S

D
|
D

M
x
L

R
|
R

A

V

I

L

C

E

Q

K

G

A

I

W

K

H

E

E

G

G

N

H

Q

E

R

R

A

A

K

R

L

L

A

A

F

I

D

K

M

T

F

F

I

V

H

H

R

R

L

I

H

A

S

R

C

H

L

I

G

A

S

G

M

H

L

A

T

A

S

S

L

L

G

H

G

R

L

L

D

D

A

G

L

I

V

I

F

F

T

T

A

G

G
|
G

I
|
I

G

G

E

E

N
|
N

S

S

T

V

I

L

V

I

R

R

E

Q

K

L

A

V

C

I

E

E

A

H

L

L

S

G

F

V

L

L

G

G

L

L

E

T

L

L

D

D

L

V

F

E

K

M

N

N

N

K

-

Q

-

P

D

N

S

S

P

H

V

G

G

E

V

R

D

I

I

I

S

S

S

A

S

N

D

P

S

S

S

Q

I

V

K

I

V

C

F

A

V

V

I

I

Q

P

T

T

Q

N

E
|
E

D

E

W

K

A

M

I

I

A

A

Q

L

S

D

S

A

W

I

Q

H

L

L

Y

G

N

N

active site: R83 (= R98), H172 (= H186), R233 (= R247), E378 (= E390)
binding adenosine-5'-triphosphate: H172 (= H186), H200 (= H214), N203 (= N217), G204 (= G218), D275 (= D289), L276 (≠ M290), R277 (= R291), G323 (= G337), I324 (= I338), N327 (= N341)
binding 1,2-ethanediol: H172 (= H186), R233 (= R247)

Sites not aligning to the query:

active site: 8

4fwkA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with amp (see paper)
41% identity, 78% coverage: 87:403/407 of query aligns to 72:391/394 of 4fwkA

query
sites
4fwkA

D

D

N

L

F

T

S

D

A

S

I

V

N

A

L

L

V

I

G

G

H

H

R
|
R

V

I

V

A

H

H

G

G

T

E

N

L

Y

F

S

T

E

Q

A

S

T

V

L

I

I

I

N

T

S

D

D

E

V

I

K

I

A
x
D

T
x
N

I

I

T

R

E
x
R

L

V

I

S

P

P

L

L

A

A

P

P

T

L

H

H

N

N

P

Y

A

A

H

N

L

L

E

S

G

G

I

I

E

D

I

A

E

R

S

H

I

L

L

F

N

P

N

A

I

V

P

R

Q

Q

V

V

A

A

V

V

F

F

D

D

T

T

A

S

F

F

H

H

S

Q

Q

T

M

L

P

A

L

P

E

E

N

A

A

Y

A

L

Y

Y

P

G

I

L

P

P

Y

W

Q

E

W

Y

L

F

E

S

K

S

-

L

G

G

I

V

R

R

Y

Y

G

G

F

F

H
|
H

G

G

I

T

S

S

H

H

Q

R

Y

Y

C

V

A

S

N

R

R

R

V

A

A

Y

E

E

L

L

M

L

N

D

Q

L

P

D

L

E

S

K

S

D

L

S

K

G

L

L

I

I

T

V

C

A

H

H

L

L

G
|
G

N
|
N

G

G

C

A

S

S

L

I

A

C

A

A

I

V

K

R

N

N

G

G

I

Q

S

S

I

V

D

D

T

T

T

S

M

M

G

G

F

M

T

T

P

P

L

L

E

E

G

G

L

L

M

M

G

G

T

T

R
|
R

G

S

G

G

S

D

I

V

D

D

P

F

A

G

I

A

L

M

I

A

Y

W

L

I

M

A

R

K

E

E

H

T

N

G

F

Q

Q

T

A

L

E

S

E

D

L

L

N

E

H

R

L

V

N

N

Q

K

E

E

S

S

G

G

L

L

K

L

G

G

I

I

S

S

G

G

I

L

S

S

D
|
D

M
x
L

R
|
R

A

V

I

L

C

E

Q

K

G

A

I

W

K

H

E

E

G

G

N

H

Q

E

R

R

A

A

K

R

L

L

A

A

F

I

D

K

M

T

F

F

I

V

H

H

R

R

L

I

H

A

S

R

C

H

L

I

G

A

S

G

M

H

L

A

T

A

S

S

L

L

G

H

G

R

L

L

D

D

A

G

L

I

V

I

F

F

T

T

A

G

G
|
G

I
|
I

G

G

E

E

N
|
N

S

S

T

V

I

L

V

I

R

R

E

Q

K

L

A

V

C

I

E

E

A

H

L

L

S

G

F

V

L

L

G

G

L

L

E

T

L

L

D

D

L

V

F

E

K

M

N

N

N

K

-

Q

-

P

D

N

S

S

P

H

V

G

G

E

V

R

D

I

I

I

S

S

S

A

S

N

D

P

S

S

S

Q

I

V

K

I

V

C

F

A

V

V

I

I

Q

P

T

T

Q

N

E
|
E

D

E

W

K

A

M

I

I

A

A

Q

L

S

D

S

A

W

I

Q

H

L

L

Y

G

N

N

active site: R83 (= R98), H172 (= H186), R233 (= R247), E378 (= E390)
binding adenosine monophosphate: G202 (= G216), N203 (= N217), D275 (= D289), L276 (≠ M290), R277 (= R291), G323 (= G337), I324 (= I338), N327 (= N341)
binding 1,2-ethanediol: D103 (≠ A118), N104 (≠ T119), R107 (≠ E122)

Sites not aligning to the query:

active site: 8

2e1zA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with diadenosine tetraphosphate (ap4a) obtained after co- crystallization with atp (see paper)
41% identity, 78% coverage: 87:403/407 of query aligns to 72:391/394 of 2e1zA

query
sites
2e1zA

D

D

N

L

F

T

S

D

A

S

I

V

N

A

L

L

V

I

G

G

H

H

R
|
R

V

I

V

A

H

H

G

G

T

E

N

L

Y

F

S

T

E

Q

A

S

T

V

L

I

I

I

N

T

S

D

D

E

V

I

K

I

A

D

T

N

I

I

T

R

E

R

L

V

I

S

P

P

L

L

A

A

P

P

T

L

H
|
H

N

N

P

Y

A

A

H

N

L

L

E

S

G

G

I

I

E

D

I

A

E

R

S

H

I

L

L

F

N

P

N

A

I

V

P

R

Q

Q

V

V

A

A

V

V

F

F

D

D

T

T

A

S

F

F

H

H

S

Q

Q

T

M

L

P

A

L

P

E

E

N

A

A

Y

A

L

Y

Y

P

G

I

L

P

P

Y

W

Q

E

W

Y

L

F

E

S

K

S

-

L

G

G

I

V

R

R

Y

Y

G

G

F

F

H
|
H

G

G

I

T

S

S

H

H

Q

R

Y

Y

C

V

A

S

N

R

R

R

V

A

A

Y

E

E

L

L

M

L

N

D

Q

L

P

D

L

E

S

K

S

D

L

S

K

G

L

L

I

I

T

V

C

A

H

H

L

L

G
|
G

N
|
N

G
|
G

C

A

S

S

L

I

A

C

A

A

I

V

K

R

N

N

G

G

I

Q

S

S

I

V

D

D

T

T

T

S

M

M

G

G

F

M

T

T

P
|
P

L

L

E

E

G

G

L

L

M

M

G

G

T

T

R
|
R

G

S

G

G

S

D

I

V

D

D

P

F

A

G

I

A

L

M

I

A

Y

W

L

I

M

A

R

K

E

E

H

T

N

G

F

Q

Q

T

A

L

E

S

E

D

L

L

N

E

H

R

L

V

N

N

Q

K

E

E

S

S

G

G

L

L

K

L

G

G

I

I

S

S

G

G

I

L

S

S

D
|
D

M
x
L

R
|
R

A

V

I

L

C

E

Q

K

G

A

I

W

K

H

E

E

G

G

N

H

Q

E

R

R

A

A

K

R

L

L

A

A

F

I

D

K

M

T

F

F

I

V

H

H

R

R

L

I

H

A

S

R

C

H

L

I

G

A

S

G

M

H

L

A

T

A

S

S

L

L

G

H

G

R

L

L

D

D

A

G

L

I

V

I

F

F

T

T

A

G

G
|
G

I
|
I

G

G

E

E

N
|
N

S

S

T

V

I

L

V

I

R

R

E

Q

K

L

A

V

C

I

E

E

A

H

L

L

S

G

F

V

L

L

G

G

L

L

E

T

L

L

D

D

L

V

F

E

K

M

N

N

N

K

-

Q

-

P

D

N

S

S

P

H

V

G

G

E

V

R

D

I

I

I

S

S

S

A

S

N

D

P

S

S

S

Q

I

V

K

I

V

C

F

A

V

V

I

I

Q

P

T

T

Q

N

E
|
E

D

E

W

K

A

M

I

I

A

A

Q

L

S

D

S

A

W

I

Q

H

L

L

Y

G

N

N

active site: R83 (= R98), H172 (= H186), R233 (= R247), E378 (= E390)
binding bis(adenosine)-5'-tetraphosphate: R83 (= R98), H115 (= H130), G202 (= G216), N203 (= N217), G204 (= G218), P224 (= P238), R233 (= R247), D275 (= D289), L276 (≠ M290), R277 (= R291), G323 (= G337), I324 (= I338), N327 (= N341)

Sites not aligning to the query:

active site: 8
binding bis(adenosine)-5'-tetraphosphate: 8

1x3nA Crystal structure of amppnp bound propionate kinase (tdcd) from salmonella typhimurium (see paper)
41% identity, 78% coverage: 87:403/407 of query aligns to 72:391/394 of 1x3nA

query
sites
1x3nA

D

D

N

L

F

T

S

D

A

S

I

V

N

A

L

L

V

I

G

G

H

H

R
|
R

V

I

V

A

H

H

G

G

T

E

N

L

Y

F

S

T

E

Q

A

S

T

V

L

I

I

I

N

T

S

D

D

E

V

I

K

I

A

D

T

N

I

I

T

R

E

R

L

V

I

S

P

P

L

L

A

A

P

P

T

L

H

H

N

N

P

Y

A

A

H

N

L

L

E

S

G

G

I

I

E

D

I

A

E

R

S

H

I

L

L

F

N

P

N

A

I

V

P

R

Q

Q

V

V

A

A

V

V

F

F

D

D

T

T

A

S

F

F

H

H

S

Q

Q

T

M

L

P

A

L

P

E

E

N

A

A

Y

A

L

Y

Y

P

G

I

L

P

P

Y

W

Q

E

W

Y

L

F

E

S

K

S

-

L

G

G

I

V

R

R

Y

Y

G

G

F

F

H
|
H

G

G

I

T

S

S

H

H

Q

R

Y

Y

C

V

A

S

N

R

R

R

V

A

A

Y

E

E

L

L

M

L

N

D

Q

L

P

D

L

E

S

K

S

D

L

S

K

G

L

L

I

I

T

V

C

A

H

H

L

L

G
|
G

N
|
N

G
|
G

C

A

S

S

L

I

A

C

A

A

I

V

K

R

N

N

G

G

I

Q

S

S

I

V

D

D

T

T

T

S

M

M

G

G

F

M

T

T

P

P

L

L

E

E

G

G

L

L

M

M

G

G

T

T

R
|
R

G

S

G

G

S

D

I

V

D

D

P

F

A

G

I

A

L

M

I

A

Y

W

L

I

M

A

R

K

E

E

H

T

N

G

F

Q

Q

T

A

L

E

S

E

D

L

L

N

E

H

R

L

V

N

N

Q

K

E

E

S

S

G

G

L

L

K

L

G

G

I

I

S

S

G

G

I

L

S

S

D
|
D

M
x
L

R
|
R

A

V

I

L

C

E

Q

K

G

A

I

W

K

H

E

E

G

G

N

H

Q

E

R

R

A

A

K

R

L

L

A

A

F

I

D

K

M

T

F

F

I

V

H

H

R

R

L

I

H

A

S

R

C

H

L

I

G

A

S

G

M

H

L

A

T

A

S

S

L

L

G

H

G

R

L

L

D

D

A

G

L

I

V

I

F

F

T

T

A

G

G
|
G

I
|
I

G

G

E

E

N
|
N

S

S

T

V

I

L

V

I

R

R

E

Q

K

L

A

V

C

I

E

E

A

H

L

L

S

G

F

V

L

L

G

G

L

L

E

T

L

L

D

D

L

V

F

E

K

M

N

N

N

K

-

Q

-

P

D

N

S

S

P

H

V

G

G

E

V

R

D

I

I

I

S

S

S

A

S

N

D

P

S

S

S

Q

I

V

K

I

V

C

F

A

V

V

I

I

Q

P

T

T

Q

N

E
|
E

D

E

W

K

A

M

I

I

A

A

Q

L

S

D

S

A

W

I

Q

H

L

L

Y

G

N

N

active site: R83 (= R98), H172 (= H186), R233 (= R247), E378 (= E390)
binding phosphoaminophosphonic acid-adenylate ester: G202 (= G216), N203 (= N217), G204 (= G218), D275 (= D289), L276 (≠ M290), R277 (= R291), G323 (= G337), I324 (= I338), N327 (= N341)

Sites not aligning to the query:

active site: 8

1x3mA Crystal structure of adp bound propionate kinase (tdcd) from salmonella typhimurium (see paper)
41% identity, 78% coverage: 87:403/407 of query aligns to 72:391/394 of 1x3mA

query
sites
1x3mA

D

D

N

L

F

T

S

D

A

S

I

V

N

A

L

L

V

I

G

G

H

H

R
|
R

V

I

V

A

H

H

G

G

T

E

N

L

Y

F

S

T

E

Q

A

S

T

V

L

I

I

I

N

T

S

D

D

E

V

I

K

I

A

D

T

N

I

I

T

R

E

R

L

V

I

S

P

P

L

L

A

A

P

P

T

L

H

H

N

N

P

Y

A

A

H

N

L

L

E

S

G

G

I

I

E

D

I

A

E

R

S

H

I

L

L

F

N

P

N

A

I

V

P

R

Q

Q

V

V

A

A

V

V

F

F

D

D

T

T

A

S

F

F

H

H

S

Q

Q

T

M

L

P

A

L

P

E

E

N

A

A

Y

A

L

Y

Y

P

G

I

L

P

P

Y

W

Q

E

W

Y

L

F

E

S

K

S

-

L

G

G

I

V

R

R

Y

Y

G

G

F

F

H
|
H

G

G

I

T

S

S

H

H

Q

R

Y

Y

C

V

A

S

N

R

R

R

V

A

A

Y

E

E

L

L

M

L

N

D

Q

L

P

D

L

E

S

K

S

D

L

S

K

G

L

L

I

I

T

V

C

A

H

H

L

L

G
|
G

N
|
N

G

G

C

A

S

S

L

I

A

C

A

A

I

V

K

R

N

N

G

G

I

Q

S

S

I

V

D

D

T

T

T

S

M

M

G

G

F

M

T

T

P

P

L

L

E

E

G

G

L

L

M

M

G

G

T

T

R
|
R

G

S

G

G

S

D

I

V

D

D

P

F

A

G

I

A

L

M

I

A

Y

W

L

I

M

A

R

K

E

E

H

T

N

G

F

Q

Q

T

A

L

E

S

E

D

L

L

N

E

H

R

L

V

N

N

Q

K

E

E

S

S

G

G

L

L

K

L

G

G

I

I

S

S

G

G

I

L

S

S

D
|
D

M
x
L

R
|
R

A

V

I

L

C

E

Q

K

G

A

I

W

K

H

E

E

G

G

N

H

Q

E

R

R

A

A

K

R

L

L

A

A

F

I

D

K

M

T

F

F

I

V

H

H

R

R

L

I

H

A

S

R

C

H

L

I

G

A

S

G

M

H

L

A

T

A

S

S

L

L

G

H

G

R

L

L

D

D

A

G

L

I

V

I

F

F

T

T

A
x
G

G
|
G

I
|
I

G

G

E

E

N
|
N

S

S

T

V

I

L

V

I

R

R

E

Q

K

L

A

V

C

I

E

E

A

H

L

L

S

G

F

V

L

L

G

G

L

L

E

T

L

L

D

D

L

V

F

E

K

M

N

N

N

K

-

Q

-

P

D

N

S

S

P

H

V

G

G

E

V

R

D

I

I

I

S

S

S

A

S

N

D

P

S

S

S

Q

I

V

K

I

V

C

F

A

V

V

I

I

Q

P

T

T

Q

N

E
|
E

D

E

W

K

A

M

I

I

A

A

Q

L

S

D

S

A

W

I

Q

H

L

L

Y

G

N

N

active site: R83 (= R98), H172 (= H186), R233 (= R247), E378 (= E390)
binding adenosine-5'-diphosphate: G202 (= G216), N203 (= N217), D275 (= D289), L276 (≠ M290), R277 (= R291), G322 (≠ A336), G323 (= G337), I324 (= I338), N327 (= N341)

Sites not aligning to the query:

active site: 8

1sazA Membership in the askha superfamily: enzymological properties and crystal structure of butyrate kinase 2 from thermotoga maritima (see paper)
27% identity, 43% coverage: 181:356/407 of query aligns to 149:323/375 of 1sazA

query
sites
1sazA

R

R

R

K

Y

S

G

I

F

F

H
|
H

G

A

I

L

S

N

H

Q

Q

K

Y

T

C

V

A

A

N

K

R

E

V

V

A

A

E

R

L

M

M

M

N

N

Q

K

P

R

L

Y

S

E

L

M

K

N

L

L

I

V

T

V

C

A

H

H

L

M

G
|
G

N
x
G

G
|
G

C

I

S

S

L

I

A

A

I

H

K

R

N

K

G

G

I

R

S

V

I

I

D

D

T

V

T

N

M

N

G

A

F

L

T

D

P

G

L

-

E

D

G

G

L

P

M

F

M

T

G

P

T

E

R
|
R

G

S

G

G

S

T

I

L

D

P

P

L

A

T

I

Q

L

L

I

V

Y

D

L

L

M

C

R

F

E

S

H

G

N

K

F

F

Q

T

A

Y

E

E

L

M

N

K

H

K

L

R

L

I

N

V

Q

G

E

N

S

G

G

G

L

L

K

V

G

A

I

Y

S

L

G

G

I

-

S

T

S
|
S

D
|
D

M
x
A

R
|
R

A

E

I

V

C

V

Q

R

G

R

I

I

K

K

E

Q

G

G

N

D

Q

E

R

W

A

A

K

K

L

R

A

V

F

Y

D

R

M

A

F

M

I

A

H

Y

R

Q

L

I

H

A

S

K

C

W

L

I

G

G

S

K

M

M

L

A

T

A

S

V

L

L

G

K

G

G

-

E

L

V

D

D

A

F

L

I

V

V

F

L

T

T

A

G

G
|
G

I
x
L

G

A

E
x
H

N

E

S

K

T

E

I

F

V

L

R

V

E

P

K

W

A

I

C

T

E

K

A

R

L

V

S

S

F

F

L

I

active site: R214 (= R247)
binding phosphomethylphosphonic acid adenylate ester: H154 (= H186), G184 (= G216), G185 (≠ N217), G186 (= G218), S254 (= S288), D255 (= D289), A256 (≠ M290), R257 (= R291), G304 (= G337), L305 (≠ I338), H307 (≠ E340)

Query Sequence

>WP_024750388.1 NCBI__GCF_000017845.1:WP_024750388.1
MNILVLNAGSSSQKSCLYCLDEKELPQETQKPIWSANIDWTATSNQGVFNVKANGIKQHL
TLESNDHHQGIRQMLDTLIQGKTKVIDNFSAINLVGHRVVHGGTNYSEATLINSDVKATI
TELIPLAPTHNPAHLEGIEIIESILNNIPQVAVFDTAFHSQMPLENAAYPIPYQWLEKGI
RRYGFHGISHQYCANRVAELMNQPLSSLKLITCHLGNGCSLAAIKNGISIDTTMGFTPLE
GLMMGTRGGSIDPAILIYLMREHNFQAEELNHLLNQESGLKGISGISSDMRAICQGIKEG
NQRAKLAFDMFIHRLHSCLGSMLTSLGGLDALVFTAGIGENSTIVREKACEALSFLGLEL
DLFKNNDSPVGVDISSSDSSIKVFVIQTQEDWAIAQSSWQLYNSLSH

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory