SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_024769835.1 NCBI__GCF_000520995.1:WP_024769835.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P36204 Bifunctional PGK/TIM; EC 2.7.2.3; EC 5.3.1.1 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
52% identity, 99% coverage: 3:396/396 of query aligns to 5:397/654 of P36204

query
sites
P36204

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R36 (= R35) binding substrate
R118 (= R116) binding substrate
R151 (= R149) binding substrate

1vpeA Crystallographic analysis of phosphoglycerate kinase from the hyperthermophilic bacterium thermotoga maritima (see paper)
52% identity, 99% coverage: 3:394/396 of query aligns to 4:394/398 of 1vpeA

query
sites
1vpeA

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active site: R35 (= R35), K196 (= K197), G353 (= G353), G376 (= G376)
binding phosphoaminophosphonic acid-adenylate ester: G194 (= G195), A195 (≠ S196), K196 (= K197), K200 (= K201), G218 (= G219), A219 (≠ G220), N316 (= N316), P318 (= P318), G320 (= G320), V321 (= V321), E323 (= E323), G352 (= G352), G353 (= G353), D354 (= D354), S355 (= S355)

1phpA Structure of the adp complex of the 3-phosphoglycerate kinase from bacillus stearothermophilus at 1.65 angstroms (see paper)
50% identity, 100% coverage: 2:396/396 of query aligns to 4:394/394 of 1phpA

query
sites
1phpA

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active site: R36 (= R35), K197 (= K197), G351 (= G353), G374 (= G376)
binding adenosine-5'-diphosphate: G195 (= G195), K201 (= K201), G219 (= G219), G220 (= G220), L237 (≠ I237), N316 (= N316), P318 (= P318), G320 (= G320), V321 (= V321), E323 (= E323), G350 (= G352), D352 (= D354), S353 (= S355)

P18912 Phosphoglycerate kinase; EC 2.7.2.3 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see paper)
50% identity, 100% coverage: 2:396/396 of query aligns to 4:394/394 of P18912

query
sites
P18912

K

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R

D

H

A

I

V

V

A

D

K

K

R

V

L

A

G

D

E

V

L

L

L

G

E

V

R

Q

P

V

V

K

A

Y

K

V

T

A

N

N

E

C

A

V

V

G

G

Q

D

E

E

A

V

E

K

D

A

A

A

V

V

A

D

A

R

L

L

N

N

S

E

G

G

E

D

V

V

L

L

E

E

N

N

L

V

R

R

F

F

H

Y

P

P

N

G

E

E

T

E

S

K

G

N

D

D

A

P

I

E

F

L

A

A

E

K

Q

A

L

F

S

A

K

E

L

L

G

A

D

D

I

L

Y

Y

I

V

N

N

D

D

A

A

F

F

G

G

T

A

A

A

H

H

R

R

A

A

H

H

A

A

S

S

T

T

T

E

I

G

V

I

A

A

Q

H

F

Y

F

L

P

P

E

-

H

-

K

A

C

V

F

A

G

G

S

F

L

L

L

M

A

E

K

K

E

E

I

L

E

E

S

V

I

L

D

G

K

K

V

A

L

L

R

S

S

N

G

P

E

D

K

R

P

P

V

F

T

T

A

A

I

I

L

I

G

G

S

A

K

K

V

V

S

K

S

D

K
|
K

I

I

T

G

I

V

I

I

E

D

N

N

I

L

L

L

D

E

K

K

I

V

D

D

H

N

L

L

I

I

G

G

M

L

T

A

Y

Y

T

T

F

F

I

V

K

K

A

A

L

L

G

G

G

H

Q

D

I

V

G

G

D

K

S

S

I

L

C

L

E

E

D

E

D

D

K

K

Q

I

E

E

L

L

A

A

L

K

E

S

I

F

L

M

R

E

K

K

A

A

K

K

E

E

K

K

G

G

V

V

E

R

I

F

H

Y

L

M

P

P

V

V

D

D

V

V

L

V

G

V

A

A

D

D

A

R

F

F

D

A

N

N

N

D

A

A

N

N

T

T

K

K

V

V

A

P

V

I

N

D

T

A

I

I

P

P

D

A

G

D

W

W

Q

S

G

A

L

L

D

D

V

I

G

G

P

P

E

K

T

T

M

R

K

E

I

L

F

Y

H

R

E

D

V

V

I

I

L

R

K

E

S

S

K

K

T

L

I

V

L

V

W

W

N
|
N

G

G

P

P

L

M

G

G

V

V

F

F

E
|
E

M

M

E

D

N

A

F

F

S

A

K

H

G

G

T

T

I

K

A

A

L

I

G

A

H

E

S

A

I

L

A

A

E

E

A

A

T

L

E

D

N

T

G

-

A

-

F

Y

S

S

L

V

V

I

G

G

G
|
G

D
|
D

S
|
S

V

A

A

A

V

V

K

E

Q

K

F

F

G

G

F

L

G

A

T

D

K

K

V

M

S

D

Y

H

V

I

S

S

T

T

G

G

A

A

M

S

L

L

E

E

S

F

L

M

E

E

G

G

K

K

T

Q

L

L

P

P

G

G

I

V

A

V

A

A

I

L

E

E

N

D

E

K

K201 (= K201) binding ATP
N316 (= N316) binding ATP
E323 (= E323) binding ATP
GGDS 350:353 (= GGDS 352:355) binding ATP

P40924 Phosphoglycerate kinase; EC 2.7.2.3 from Bacillus subtilis (strain 168) (see paper)
47% identity, 100% coverage: 2:396/396 of query aligns to 4:394/394 of P40924

query
sites
P40924

K

K

T

T

I

L

D

K

D

D

F

I

N

D

F

V

E

K

N

G

K

K

K

V

A

V

L

F

I

C

R

R

V

V

D

D

F

F

N

N

V

V

P

P

L

M

N

K

E

D

N

G

F

-

E

E

V

V

T

T

D

D

T

T

R

R

I

I

Q

R

A

A

K

L

P

P

T

T

I

I

I

K

K

H

V

L

L

A

E

D

D

Q

G

G

G

A

S

K

A

V

I

L

L

L

M

A

S

S

H

H

L

L

G

G

R

R

P

P

K

K

G

G

-

E

V

V

Q

V

D

E

E

E

F

L

S

R

L

L

R

T

H

P

I

V

V

A

D

A

K

R

V

L

A

G

D

E

V

L

L

L

G

G

V

K

Q

E

V

V

K

K

Y

K

V

A

A

D

N

E

C

A

V

Y

G

G

Q

D

E

A

A

V

E

K

D

A

A

Q

V

I

A

S

A

E

L

M

N

K

S

D

G

G

E

D

V

V

L

L

L

V

L

L

E

E

N

N

L

V

R

R

F

F

H

Y

P

P

N

G

E

E

T

E

S

K

G

N

D

D

A

P

I

E

F

L

A

A

E

K

Q

A

L

F

S

A

K

E

L

L

G

A

D

D

I

V

Y

Y

I

V

N

N

D

D

A

A

F

F

G

G

T

A

A

A

H

H

R

R

A

A

H

H

A

A

S

S

T

T

T

A

I

G

V

I

A

A

Q

E

F

H

F

L

P

P

E

-

H

-

K

A

C

V

F

A

G

G

S

F

L

L

L

M

A

E

K

K

E

E

I

L

E

D

S

V

I

L

D

G

K

K

V

A

L

V

R
x
S

S

N

G

P

E

D

K

R

P

P

V

F

T

T

A

A

I

I

L

I

G

G

S

A

K

K

V

V

S

K

S

D

K

K

I

I

T

G

I

V

I

I

E

E

N

S

I

L

L

L

D

D

K

K

I

V

D

D

H

N

L

L

I

I

G

G

M

L

T

A

Y

Y

T

T

F

F

I

V

K

K

A

A

L

L

G

G

G

Y

Q

E

I

V

G

G

D

K

S

S

I

L

C

L

E

E

D

E

D

D

K

K

Q

I

E

E

L

L

A

A

L

K

E

S

I

F

L

M

R

D

K

R

A

A

K

K

E

E

K

K

G

G

V

V

E

N

I

F

H

Y

L

M

P

P

V

E

D

D

V

V

L

L

G

V

A

A

D

D

A

D

F

F

D

S

N

N

N

D

A

A

N

N

T

V

K

K

V

I

V

V

A

P

V

I

N

S

T

E

I

I

P

P

D

S

G

D

W

L

Q

E

G

A

L

I

D

D

V

I

G

G

P

T

E

K

T
|
T

M

R

K

E

I

T

F

Y

H

A

E

D

V

V

I

I

L

K

K

N

S

S

K

K

T

L

I

V

L

V

W

W

N

N

G

G

P

P

L

M

G

G

V

V

F

F

E

E

M

I

E

D

N

L

F

F

S

A

K

Q

G

G

T

T

I

K

A

A

L

V

G

A

H

E

S

A

I

L

A

A

E

E

A

A

T

K

E

D

N

T

G

-

A

-

F

Y

S

S

L

V

V

I

G

G

D

D

S

S

V

A

A

A

V

V

K

E

Q

K

F

F

G

G

F

L

G

A

T

D

K

K

V

M

S

S

Y

H

V

I

S

S

T

T

G

G

A

A

M

S

L

L

E

E

S

F

L

M

E

E

G

G

K

K

T

E

L

L

P

P

G

G

I

V

A

A

I

L

E

N

N

D

E

K

S183 (≠ R183) modified: Phosphoserine
T299 (= T299) modified: Phosphothreonine

P09041 Phosphoglycerate kinase 2; Phosphoglycerate kinase, testis specific; EC 2.7.2.3 from Mus musculus (Mouse) (see paper)
46% identity, 99% coverage: 3:395/396 of query aligns to 8:416/417 of P09041

query
sites
P09041

T

T

I

L

D

D

D

K

F

V

N

D

F

L

E

K

N

G

K

K

K

R

A

V

L

I

I

M

R

R

V

V

D

D

F

F

N

N

V

V

P

P

L

M

N

K

E

N

N

N

F

-

E

Q

V

I

T

T

D

N

T

Q

R

R

I

I

Q

K

A

A

K

I

P

P

T

S

I

I

I

K

K

H

V

C

L

L

E

D

D

N

G

G

G

A

-

K

S

S

A

V

I

V

L

L

M

M

S

S

H

H

L

L

G

G

R

R

P

P

K

D

G

G

V

I

Q

P

-

M

-

P

D

D

E

K

F

Y

S

S

L

L

R

E

H

P

I

V

V

A

D

D

K

E

V

L

A

K

D

S

V

L

L

L

G

N

V

K

Q

D

V

V

K

I

Y

F

V

L

A

K

N

D

C

C

V

V

G

G

Q

P

E

E

A

V

E

E

D

Q

A

A

V

C

A

A

A

N

L

P

N

D

S

N

G

G

E

S

V

I

L

I

L

L

E

E

N

N

L

L

R

R

F

F

H

H

P

V

N

E

E

E

-

E

-

G

-

K

-

G

-

K

-

D

T

S

S

S

G

G

D

K

A

K

I

I

-

S

-

A

-

D

-

P

-

A

-

K

-

V

-

E

-

A

F

F

A

Q

E

A

Q

S

L

L

S

S

K

K

L

L

G

G

D

D

I

V

Y

Y

I

V

N

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

A

H

H

A

S

S

S

T

T

T

-

I

-

V

V

A

G

Q

V

F

N

F

L

P

P

E

Q

H

-

K

K

C

A

F

S

G

G

S

F

L

L

L

M

A

K

K

K

E

E

I

L

E

D

S

Y

I

F

D

S

K

K

V

A

L

L

R

E

S

K

G

P

E

E

K

R

P

P

V

F

T

L

A

A

I

I

L

L

G

G

S

A

K

K

V

V

S

K

S

D

K
|
K

I

I

T

Q

I

L

I

I

E

K

N

N

I

M

L

L

D

D

K

K

I

V

D

N

H

F

L

M

I

I

G

G

M

M

T

A

Y

Y

T

T

F

F

I

L

K

K

A

E

L

L

G

K

G

N

-

M

Q

Q

I

I

G

G

D

A

S

S

I

L

C

F

E

D

D

E

K

G

Q

A

E

T

L

I

A

V

L

K

E

E

I

I

L

M

R

E

K

K

A

A

K

E

E

K

K

N

G

G

V

V

E

K

I

I

H

V

L

F

P

P

V

V

D

D

V

F

L

V

G

T

A

G

D

D

A

K

F

F

D

D

N

E

N

N

A

A

N

K

T

V

K

G

V

Q

V

A

A

T

V

I

N

E

T

S

-

G

I

I

P

P

D

S

G

G

W

W

Q

M

G
|
G

L

L

D

D

V

C

G

G

P

P

E

E

T

S

M

I

K

K

I

I

F

N

H

A

E

Q

V

I

I

V

L

A

K

Q

S

A

K

K

T

L

I

I

L

V

W

W

N

N

G

G

P

P

L

I

G

G

V

V

F

F

E
|
E

M

W

E

D

N

A

F

F

S

A

K

K

G

G

T

T

I

K

A

A

L

L

G

M

H

D

S

E

I

V

A

V

E

K

A

A

T

T

E

S

N

N

G

G

A

C

F

V

S

T

L

I

V

I

G

G

G
|
G

D
|
D

S
x
T

V

A

A

T

A

C

V

C

K

A

Q

K

F

W

G

G

F

T

G

E

T

D

K

K

V

V

S

S

Y

H

V

V

S

S

T

T

G

G

A

A

M

S

L

L

E

E

S

L

L

L

E

E

G

G

K

K

T

I

L

L

P

P

G

G

I

V

A

E

A

A

I

L

E

S

N

N

K220 (= K201) binding ATP
G313 (= G292) binding ATP
E344 (= E323) binding ATP
GGDT 373:376 (≠ GGDS 352:355) binding ATP

2paaA Crystal structure of phosphoglycerate kinase-2 bound to atp and 3pg (see paper)
46% identity, 99% coverage: 3:395/396 of query aligns to 4:412/413 of 2paaA

query
sites
2paaA

T

T

I

L

D

D

D

K

F

V

N

D

F

L

E

K

N

G

K

K

K

R

A

V

L

I

I

M

R

R

V

V

D

D

F

F

N

N

V

V

P

P

L

M

N

K

E

N

N

N

F

-

E

Q

V

I

T

T

D

N

T

Q

R
|
R

I

I

Q

K

A

A

K

I

P

P

T

S

I

I

I

K

K

H

V

C

L

L

E

D

D

N

G

G

G

A

-

K

S

S

A

V

I

V

L

L

M

M

S

S

H

H

L

L

G

G

R

R

P

P

K

D

G

G

V

I

Q

P

-

M

-

P

D

D

E

K

F

Y

S

S

L

L

R

E

H

P

I

V

V

A

D

D

K

E

V

L

A

K

D

S

V

L

L

L

G

N

V

K

Q

D

V

V

K

I

Y

F

V

L

A

K

N

D

C

C

V

V

G

G

Q

P

E

E

A

V

E

E

D

Q

A

A

V

C

A

A

A

N

L

P

N

D

S

N

G

G

E

S

V

I

L

I

L

L

E

E

N

N

L

L

R

R

F

F

H

H

P

V

N

E

E

E

-

E

-

G

-

K

-

G

-

K

-

D

T

S

S

S

G

G

D

K

A

K

I

I

-

S

-

A

-

D

-

P

-

A

-

K

-

V

-

E

-

A

F

F

A

R

E

A

Q

S

L

L

S

S

K

K

L

L

G

G

D

D

I

V

Y

Y

I

V

N

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

A

H

H

A

S

S

S

T

-

I

M

V

V

A

G

Q

V

F

N

F

L

P

P

E

Q

H

-

K

K

C

A

F

S

G

G

S

F

L

L

L

M

A

K

K

K

E

E

I

L

E

D

S

Y

I

F

D

S

K

K

V

A

L

L

R

E

S

K

G

P

E

E

K

R

P

P

V

F

T

L

A

A

I

I

L

L

G

G

G
|
G

S
x
A

K
|
K

V

V

S

K

S

D

K
|
K

I

I

T

Q

I

L

I

I

E

K

N

N

I

M

L

L

D

D

K

K

I

V

D

N

H

F

L

M

I

I

G

G

G
|
G

M

M

T

A

Y

Y

T

T

F

F

I

L

K

K

A

E

L

L

G

K

G

N

-

M

Q

Q

I

I

G

G

D

A

S

S

I
x
L

C

F

E

D

D

E

K

G

Q

A

E

T

L

I

A

V

L

K

E

E

I

I

L

M

R

E

K

K

A

A

K

E

E

K

K

N

G

G

V

V

E

K

I

I

H

V

L

F

P

P

V

V

D

D

V

F

L

V

G

T

A

G

D

D

A

K

F

F

D

D

N

E

N

N

A

A

N

K

T

V

K

G

V

Q

V

A

A

T

V

I

N

E

T

S

-

G

I

I

P

P

D

S

G

G

W

W

Q

M

G
|
G

L
|
L

D

D

V

C

G

G

P

P

E

E

T

S

M

I

K

K

I

I

F

N

H

A

E

Q

V

I

I

V

L

A

K

Q

S

A

K

K

T

L

I

I

L

V

W

W

N

N

G
|
G

P

P

L

I

G
|
G

V
|
V

F

F

E
|
E

M

W

E

D

N

A

F

F

S

A

K

K

G

G

T

T

I

K

A

A

L

L

G

M

H

D

S

E

I

V

A

V

E

K

A

A

T

T

E

S

N

N

G

G

A

C

F

V

S

T

L

I

V

I

G

G

G
|
G

D
|
D

S

T

V

A

A

T

A

C

V

C

K

A

Q

K

F

W

G

G

F

T

G

E

T

D

K

K

V

V

S

S

Y

H

V

V

S

S

T

T

G

G

G
|
G

A

A

M

S

L

L

E

E

S

L

L

L

E

E

G

G

K

K

T

I

L

L

P

P

G

G

I

V

A

E

A

A

I

L

E

S

N

N

active site: R35 (= R35), K212 (= K197), G370 (= G353), G393 (= G376)
binding adenosine-5'-triphosphate: G210 (= G195), A211 (≠ S196), K216 (= K201), G235 (= G220), L253 (≠ I237), G309 (= G292), L310 (= L293), G334 (= G317), G337 (= G320), V338 (= V321), E340 (= E323), D371 (= D354)

1ltkC Crystal structure of phosphoglycerate kinase from plasmodium falciparum, in the open conformation
47% identity, 99% coverage: 3:396/396 of query aligns to 15:424/424 of 1ltkC

query
sites
1ltkC

T

S

I

I

D

S

D

D

F

L

-

K

N

D

F

I

E

K

N

N

K

K

A

V

L

L

I

V

R

R

V

V

D

D

F

F

N

N

V

V

P

P

L

I

N

-

E

E

N

N

F

G

E

I

V

I

T

K

D

D

D

T

T

N

R
|
R

I

I

Q

T

A

A

A

T

K

L

P

P

T

T

I

I

I

N

K

H

V

L

L

K

E

K

D

E

G

G

G

A

S

S

A

K

I

I

-

I

L

L

M

I

S

S

H

H

L

C

G

G

R

R

P

P

K

D

G

G

V

L

Q

R

D

N

E

E

-

K

F

Y

S

T

L

L

R

K

H

P

I

V

V

A

D

E

K

T

V

L

A

K

D

G

V

L

L

L

G

G

V

E

Q

E

V

V

K

L

Y

F

V

L

A

N

N

D

C

C

V

V

G

G

Q

K

E

E

A

V

E

E

D

D

A

K

V

I

A

N

A

A

L

A

N

K

S

E

G

N

E

S

V

V

L

I

L

L

E

E

N

N

L

L

R

R

F

F

H

H

P

I

N

E

E

E

-

E

-

G

-

K

-

G

-

V

-

D

T

A

S

N

G

G

D

N

A

K

I

V

-

K

-

A

-

N

-

K

-

E

-

D

-

V

-

E

-

K

F

F

A

Q

E

N

Q

D

L

L

S

T

K

K

L

L

G

A

D

D

I

V

Y

F

I

I

N

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

A

H

H

A

S

S

S

T

M

T

V

I

G

V

V

A

K

Q

L

F

N

F

V

P

-

E

-

H

-

K

K

C

A

F

S

G

G

S

F

L

L

L

M

A

K

K

K

E

E

I

L

E

E

S

Y

I

F

D

S

K

K

V

A

L

L

R

E

S

N

G

P

E

Q

K

R

P

P

V

L

T

L

A

A

I

I

L

L

G

G

G
|
G

S
x
A

K
|
K

V

V

S

S

S

D

K

K

I

I

T

Q

I

L

I

I

E

K

N

N

I

L

L

L

D

D

K

K

I

V

D

D

H

R

L

M

I

I

G

G

G
|
G

M

M

T

A

Y

Y

T

T

F

F

I

K

K

K

A

V

L

L

G

N

-

M

Q

K

I

I

G

G

D

T

S

S

I

L

C

F

E

D

D

E

D

A

K

G

Q

S

E

K

L

I

A

V

L

G

E

E

I

I

L

M

R

E

K

K

A

A

K

K

E

A

K

K

G

N

V

V

E

Q

I

I

H

F

L

L

P

P

V

V

D

D

V

F

L

K

G

I

A

A

D

D

A

N

F

F

D

D

N

N

A

A

N

N

T

T

K

K

V

F

V

V

A

T

V

D

N

E

T

E

-

G

I

I

P

P

D

D

G

N

W

W

Q

M

G

G

L

L

D

D

V

A

G

G

P

P

E

K

T

S

M

I

K

E

I

N

F

Y

H

K

E

D

V

V

I

I

L

L

K

T

S

S

K

K

T

T

I

V

L

I

W

W

N

N

G

G

P

P

L

Q

G
|
G

V
|
V

F

F

E
|
E

M

M

E

P

N

N

F

F

S

A

K

K

G

G

T

S

I

I

A

E

L

C

G

L

H

N

S

L

I

V

A

V

E

E

A

V

T

T

E

K

N

K

G

G

A

A

F

I

S

T

L

I

V

V

G

G

G
|
G

D
|
D

S

T

V

A

A

S

A

L

V

V

K

E

Q

Q

F

Q

G

N

F

K

G

K

T

N

K

E

V

I

S

S

Y

H

V

V

S

S

T

T

G

G

G
|
G

A

A

M

S

L

L

E

E

S

L

L

L

E

E

G

G

K

K

T

E

L

L

P

P

G

G

I

V

A

L

A

A

I

L

E

S

N

N

E

K

active site: R47 (= R35), K223 (= K197), G381 (= G353), G404 (= G376)
binding adenosine monophosphate: G221 (= G195), A222 (≠ S196), K223 (= K197), G245 (= G219), G246 (= G220), G348 (= G320), V349 (= V321), E351 (= E323), D382 (= D354)

2wzcA The catalytically active fully closed conformation of human phosphoglycerate kinase in complex with adp, 3pg and aluminium tetrafluoride (see paper)
46% identity, 99% coverage: 3:395/396 of query aligns to 6:404/405 of 2wzcA

query
sites
2wzcA

T

T

I

L

D

D

D

K

F

L

N

D

F

V

E

K

N

G

K

K

K

R

A

V

L

V

I

M

R

R

V

V

D

D

F

F

N

N

V

V

P

P

L

M

N

K

E

N

N

N

F

-

E

Q

V

I

T

T

D

N

T

Q

R
|
R

I

I

Q

K

A

A

K

V

P

P

T

S

I

I

I

K

K

F

V

C

L

L

E

D

D

N

G

G

G

A

-

K

S

S

A

V

I

V

L

L

M

M

S

S

H

H

L

L

G

G

R

R

P

P

K

D

G

G

V

V

Q

P

-

M

-

P

D

D

E

K

F

Y

S

S

L

L

R

E

H

P

I

V

V

A

D

V

K

E

V

L

A

K

D

S

V

L

L

L

G

G

V

K

Q

D

V

V

K

L

Y

F

V

L

A

K

N

D

C

C

V

V

G

G

Q

P

E

E

A

V

E

E

D

K

A

A

V

C

A

A

A

N

L

P

N

A

S

A

G

G

E

S

V

V

L

I

L

L

E

E

N

N

L

L

R

R

F

F

H

H

-

V

-

E

-

G

P

K

N

G

E

E

T

P

S

A

G

K

D

I

A

E

I

A

F

F

A

R

E

A

Q

S

L

L

S

S

K

K

L

L

G

G

D

D

I

V

Y

Y

I

V

N

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

A

H

H

A

S

S

S

T

-

I

M

V

V

A

G

Q

V

F

N

F

L

P

P

E

Q

H

-

K

K

C

A

F

G

G

G

S

F

L

L

L

M

A

K

K

K

E

E

I

L

E

N

S

Y

I

F

D

A

K

K

V

A

L

L

R

E

S

S

G

P

E

E

K

R

P

P

V

F

T

L

A

A

I

I

L

L

G

G

G
|
G

S
x
A

K
|
K

V

V

S

A

S

D

K
|
K

I

I

T

Q

I

L

I

I

E

N

N

N

I

M

L

L

D

D

K

K

I

V

D

N

H

E

L

M

I

I

G

G

G
|
G

M

M

T

A

Y

F

T

T

F

F

I

L

K

K

A

V

L

L

G

N

-

M

Q

E

I

I

G

G

D

T

S

S

I

L

C

F

E

D

D

E

K

G

Q

A

E

K

L

I

A

V

L

K

E

D

I

L

L

M

R

S

K

K

A

A

K

E

E

K

K

N

G

G

V

V

E

K

I

I

H

T

L

L

P

P

V

V

D

D

V

F

L

V

G

T

A

A

D

D

A

K

F

F

D

D

N

E

N

N

A

A

N

K

T

T

K

G

V

Q

V

A

A

T

V

V

N

A

T

S

-

G

I

I

P

P

D

A

G

G

W

W

Q

M

G

G

L

L

D

D

V

C

G

G

P

P

E

E

T

S

M

S

K

K

I

K

F

Y

H

A

E

E

V

A

I

V

L

T

K

R

S

A

K

K

T

Q

I

I

L

V

W

W

N
|
N

G

G

P
|
P

L

V

G
|
G

V
|
V

F

F

E
|
E

M

W

E

E

N

A

F

F

S

A

K

R

G

G

T

T

I

K

A

A

L

L

G

M

H

D

S

E

I

V

A

V

E

K

A

A

T

T

E

S

N

R

G

G

A

C

F

I

S

T

L

I

V

I

G

G

G
|
G

D
|
D

S
x
T

V

A

A

T

A

C

V

C

K

A

Q

K

F

W

G

N

F

T

G

E

T

D

K

K

V

V

S

S

Y

H

V

V

S

S

T

T

G

G

G
|
G

A

A

M

S

L

L

E

E

S

L

L

L

E

E

G

G

K

K

T

V

L

L

P

P

G

G

I

V

A

D

A

A

I

L

E

S

N

N

active site: R37 (= R35), K204 (= K197), G362 (= G353), G385 (= G376)
binding adenosine-5'-diphosphate: G202 (= G195), A203 (≠ S196), K204 (= K197), K208 (= K201), G226 (= G219), G227 (= G220), N325 (= N316), P327 (= P318), G329 (= G320), V330 (= V321), E332 (= E323), G361 (= G352), D363 (= D354), T364 (≠ S355)
binding tetrafluoroaluminate ion: R37 (= R35), K204 (= K197), K208 (= K201), G361 (= G352), G362 (= G353), G384 (= G375)

2wzbA The catalytically active fully closed conformation of human phosphoglycerate kinase in complex with adp, 3pg and magnesium trifluoride (see paper)
46% identity, 99% coverage: 3:395/396 of query aligns to 6:404/405 of 2wzbA

query
sites
2wzbA

T

T

I

L

D

D

D

K

F

L

N

D

F

V

E

K

N

G

K

K

K

R

A

V

L

V

I

M

R

R

V

V

D

D

F

F

N

N

V

V

P

P

L

M

N

K

E

N

N

N

F

-

E

Q

V

I

T

T

D

N

T

Q

R
|
R

I

I

Q

K

A

A

K

V

P

P

T

S

I

I

I

K

K

F

V

C

L

L

E

D

D

N

G

G

G

A

-

K

S

S

A

V

I

V

L

L

M

M

S

S

H

H

L

L

G

G

R

R

P

P

K

D

G

G

V

V

Q

P

-

M

-

P

D

D

E

K

F

Y

S

S

L

L

R

E

H

P

I

V

V

A

D

V

K

E

V

L

A

K

D

S

V

L

L

L

G

G

V

K

Q

D

V

V

K

L

Y

F

V

L

A

K

N

D

C

C

V

V

G

G

Q

P

E

E

A

V

E

E

D

K

A

A

V

C

A

A

A

N

L

P

N

A

S

A

G

G

E

S

V

V

L

I

L

L

E

E

N

N

L

L

R

R

F

F

H

H

-

V

-

E

-

G

P

K

N

G

E

E

T

P

S

A

G

K

D

I

A

E

I

A

F

F

A

R

E

A

Q

S

L

L

S

S

K

K

L

L

G

G

D

D

I

V

Y

Y

I

V

N

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

A

H

H

A

S

S

S

T

-

I

M

V

V

A

G

Q

V

F

N

F

L

P

P

E

Q

H

-

K

K

C

A

F

G

G

G

S

F

L

L

L

M

A

K

K

K

E

E

I

L

E

N

S

Y

I

F

D

A

K

K

V

A

L

L

R

E

S

S

G

P

E

E

K

R

P

P

V

F

T

L

A

A

I

I

L

L

G

G

G
|
G

S
x
A

K
|
K

V

V

S

A

S

D

K
|
K

I

I

T

Q

I

L

I

I

E

N

N

N

I

M

L

L

D

D

K

K

I

V

D

N

H

E

L

M

I

I

G

G

G
|
G

M

M

T

A

Y

F

T

T

F

F

I

L

K

K

A

V

L

L

G

N

-

M

Q

E

I

I

G

G

D

T

S

S

I

L

C

F

E

D

D

E

K

G

Q

A

E

K

L

I

A

V

L

K

E

D

I

L

L

M

R

S

K

K

A

A

K

E

E

K

K

N

G

G

V

V

E

K

I

I

H

T

L

L

P

P

V

V

D

D

V

F

L

V

G

T

A

A

D

D

A

K

F

F

D

D

N

E

N

N

A

A

N

K

T

T

K

G

V

Q

V

A

A

T

V

V

N

A

T

S

-

G

I

I

P

P

D

A

G

G

W

W

Q

M

G

G

L

L

D

D

V

C

G

G

P

P

E

E

T

S

M

S

K

K

I

K

F

Y

H

A

E

E

V

A

I

V

L

T

K

R

S

A

K

K

T

Q

I

I

L

V

W

W

N
|
N

G

G

P
|
P

L

V

G
|
G

V
|
V

F

F

E
|
E

M

W

E

E

N

A

F

F

S

A

K

R

G

G

T

T

I

K

A

A

L

L

G

M

H

D

S

E

I

V

A

V

E

K

A

A

T

T

E

S

N

R

G

G

A

C

F

I

S

T

L

I

V

I

G

G

G
|
G

D
|
D

S
x
T

V

A

A

T

A

C

V

C

K

A

Q

K

F

W

G

N

F

T

G

E

T

D

K

K

V

V

S

S

Y

H

V

V

S

S

T

T

G

G

G
|
G

A

A

M

S

L

L

E

E

S

L

L

L

E

E

G

G

K

K

T

V

L

L

P

P

G

G

I

V

A

D

A

A

I

L

E

S

N

N

active site: R37 (= R35), K204 (= K197), G362 (= G353), G385 (= G376)
binding adenosine-5'-diphosphate: G202 (= G195), A203 (≠ S196), K204 (= K197), K208 (= K201), G226 (= G219), G227 (= G220), N325 (= N316), P327 (= P318), G329 (= G320), V330 (= V321), E332 (= E323), G361 (= G352), D363 (= D354), T364 (≠ S355)
binding trifluoromagnesate: K204 (= K197), K208 (= K201), G361 (= G352), G384 (= G375), G385 (= G376)

P00558 Phosphoglycerate kinase 1; Cell migration-inducing gene 10 protein; Primer recognition protein 2; PRP 2; EC 2.7.2.3 from Homo sapiens (Human) (see 16 papers)
46% identity, 99% coverage: 3:395/396 of query aligns to 8:416/417 of P00558

query
sites
P00558

T

T

I

L

D

D

D

K

F

L

N

D

F

V

E

K

N

G

K

K

K

R

A

V

L

V

I

M

R

R

V

V

D
|
D

F
|
F

N
|
N

V

V

P

P

L

M

N

K

E

N

N

N

F

-

E

Q

V

I

T

T

D

N

T

Q

R
|
R

I

I

Q

K

A

A

K

V

P

P

T

S

I

I

I

K

K

F

V

C

L

L

E

D

D

N

G

G

G

A

-

K

S

S

A

V

I

V

L

L

M

M

S

S

H
|
H

L
|
L

G
|
G

R
|
R

P

P

K

D

G

G

V

V

Q

P

-

M

-

P

D

D

E

K

F

Y

S

S

L

L

R

E

H

P

I

V

V

A

D

V

K

E

V

L

A

K

D

S

V
x
L

L

L

G

G

V

K

Q

D

V

V

K

L

Y

F

V

L

A
x
K

N

D

C

C

V

V

G

G

Q

P

E

E

A

V

E

E

D

K

A

A

V

C

A

A

A

N

L

P

N

A

S

A

G

G

E

S

V

V

L

I

L

L

E

E

N

N

L

L

R
|
R

F

F

H

H

P

V

N

E

E

E

-

E

-

G

-
x
K

-

G

-

K

-

D

T

A

S

S

G

G

D

N

A

K

I

V

F

K

A

A

E

E

-

P

-

A

-

K

-

I

-

E

-

A

-

F

-

R

-

A

Q

S

L

L

S

S

K

K

L

L

G
|
G

D

D

I

V

Y

Y

I

V

N

N

D
|
D

A

A

F

F

G

G

T

T

A

A

H

H

R
|
R

A

A

H

H

A

S

S

S

T

-

I

M

V

V

A

G

Q

V

F

N

F

L

P

P

E

Q

H

-

K

K

C

A

F

G

G

G

S

F

L

L

L

M

A
x
K

K

K

E

E

I

L

E

N

S

Y

I

F

D

A

K

K

V

A

L

L

R

E

S

S

G

P

E

E

K
x
R

P

P

V

F

T

L

A

A

I

I

L

L

G

G

S

A

K
|
K

V

V

S

A

S

D

K
|
K

I

I

T

Q

I

L

I

I

E

N

N

N

I

M

L

L

D

D

K

K

I

V

D

N

H

E

L

M

I

I

G

G

M

M

T

A

Y

F

T

T

F

F

I

L

K

K

A

V

L

L

G

N

-

M

Q
x
E

I

I

G

G

D

T

S

S

I

L

C

F

E

D

D

E

K

G

Q

A

E

K

L

I

A
x
V

L

K

E
x
D

I

L

L

M

R

S

K

K

A

A

K

E

E

K

K

N

G

G

V

V

E

K

I

I

H

T

L

L

P

P

V

V

D
|
D

V

F

L

V

G

T

A

A

D

D

A

K

F

F

D

D

N

E

N

N

A

A

N

K

T

T

-

G

K

Q

V

A

V

T

A

V

V

A

N

S

T

G

I

I

P

P

D

A

G

G

W

W

Q

M

G
|
G

L

L

D
|
D

V
x
C

G

G

P

P

E

E

T

S

M

S

K

K

I
x
K

F

Y

H

A

E

E

V

A

I

V

L

T

K

R

S

A

K

K

T

Q

I

I

L

V

W

W

N

N

G

G

P

P

L

V

G

G

V

V

F

F

E
|
E

M

W

E

E

N

A

F

F

S

A

K

R

G

G

T
|
T

I

K

A

A

L

L

G

M

H

D

S

E

I

V

A

V

E

K

A

A

T

T

E

S

N

R

G

G

A

C

F

I

S

T

L

I

V

I

G

G

G
|
G

D
|
D

S
x
T

V

A

A

T

A

C

V

C

K

A

Q

K

F

W

G

N

F

T

G

E

T

D

K

K

V

V

S

S

Y

H

V

V

S

S

T

T

G

G

A

A

M

S

L

L

E

E

S

L

L

L

E

E

G

G

K

K

T

V

L

L

P

P

G

G

I

V

A

D

A

A

I

L

E

S

N

N

DFN 24:26 (= DFN 19:21) binding substrate
R39 (= R35) binding substrate
HLGR 63:66 (= HLGR 58:61) binding substrate
L88 (≠ V81) to P: in PGK1D; with congenital non-spherocytic anemia; variant Matsue; dbSNP:rs137852531
K97 (≠ A90) modified: N6-(2-hydroxyisobutyryl)lysine; alternate
R123 (= R116) binding substrate
K131 (vs. gap) modified: N6-malonyllysine; alternate
G158 (= G136) to V: in PGK1D; with chronic hemolytic anemia; variant Shizuoka; dbSNP:rs137852532
D164 (= D142) to V: in PGK1D; with chronic hemolytic anemia and intellectual disability; variant Amiens; dbSNP:rs137852538
R171 (= R149) binding substrate
K191 (≠ A172) natural variant: Missing (in PGK1D; with chronic hemolytic anemia; variant Alabama)
R206 (≠ K187) to P: in PGK1D; with chronic hemolytic anemia; variant Uppsala; dbSNP:rs137852529
K216 (= K197) modified: N6-(2-hydroxyisobutyryl)lysine
K220 (= K201) binding ATP; modified: N6-(2-hydroxyisobutyryl)lysine
E252 (≠ Q232) to A: in PGK1D; with chronic hemolytic anemia; variant Antwerp
V266 (≠ A246) to M: in PGK1D; with chronic non-spherocytic hemolytic anemia; variant Tokyo; dbSNP:rs431905501
D268 (≠ E248) to N: in Munchen; 21% of activity; dbSNP:rs137852528
D285 (= D265) to V: in PGK1D; with chronic hemolytic anemia; variant Herlev; 50% of activity; dbSNP:rs137852535
G313 (= G292) binding ATP
D315 (= D294) to N: in PGK1D; with rhabdomyolysis; variant Creteil
C316 (≠ V295) to R: in PGK1D; with chronic hemolytic anemia; variant Michigan; dbSNP:rs137852533
K323 (≠ I302) modified: N6-(2-hydroxyisobutyryl)lysine
E344 (= E323) binding ATP
T352 (= T331) to N: in dbSNP:rs137852530
GGDT 373:376 (≠ GGDS 352:355) binding ATP

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

2y3iA The structure of the fully closed conformation of human pgk in complex with l-adp, 3pg and the tsa aluminium tetrafluoride (see paper)
46% identity, 99% coverage: 3:395/396 of query aligns to 6:414/414 of 2y3iA

query
sites
2y3iA

T

T

I

L

D

D

D

K

F

L

N

D

F

V

E

K

N

G

K

K

K

R

A

V

L

V

I

M

R

R

V

V

D

D

F

F

N

N

V

V

P

P

L

M

N

K

E

N

N

N

F

-

E

Q

V

I

T

T

D

N

T

Q

R
|
R

I

I

Q

K

A

A

K

V

P

P

T

S

I

I

I

K

K

F

V

C