SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_024770047.1 NCBI__GCF_000520995.1:WP_024770047.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

O59010 Glutamate transporter homolog; Glt(Ph); Sodium-aspartate symporter Glt(Ph); Sodium-dependent aspartate transporter from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 3 papers)
38% identity, 95% coverage: 8:426/440 of query aligns to 14:419/425 of O59010

query
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O59010

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S65 (= S60) mutation to V: Strongly decreased chloride conductance.
R276 (≠ S283) mutation to S: Increased rate of aspartate transport; when associated with R-395.
RSS 276:278 (≠ SSS 283:285) binding L-aspartate
M311 (= M319) mutation to A: Decreased dependence of aspartate binding on Na(+) concentration.
T314 (= T322) binding L-aspartate
V355 (= V364) binding L-aspartate
D394 (= D401) binding L-aspartate
M395 (= M402) mutation to R: Increased rate of aspartate transport; when associated with S-276.
R397 (= R404) mutation to A: Strongly decreased affinity for aspartate.
N401 (= N408) binding L-aspartate
D405 (= D412) mutation to N: Strongly decreased affinity for aspartate.

6x15A Inward-facing state of the glutamate transporter homologue gltph in complex with l-aspartate and sodium ions (see paper)
38% identity, 95% coverage: 9:426/440 of query aligns to 15:419/419 of 6x15A

query
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6x15A

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binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: F46 (≠ L41), F46 (≠ L41), P75 (≠ D70), L91 (= L87), F95 (≠ I91), L130 (= L143), I133 (≠ F145), I159 (= I172), Y167 (≠ L180), K196 (= K202), G200 (≠ L206), I207 (≠ Y213), F210 (= F216), L250 (≠ V257), I262 (≠ L269), M269 (≠ Q276), T334 (= T343), V335 (≠ F344), G336 (≠ S345), T340 (= T349), L343 (= L352), M399 (≠ T406)
binding aspartic acid: S277 (= S284), S278 (= S285), T314 (= T322), G354 (≠ A363), A358 (= A367), G359 (= G368), D394 (= D401), R397 (= R404), T398 (= T405)
binding sodium ion: Y89 (= Y85), T92 (= T88), S93 (≠ T89), G306 (= G314), T308 (= T316), N310 (= N318), N310 (= N318), M311 (= M319), D312 (= D320), S349 (= S358), I350 (= I359), T352 (≠ S361), N401 (= N408), V402 (= V409), D405 (= D412)

Sites not aligning to the query:

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: 4, 7

2nwwA Crystal structure of gltph in complex with tboa (see paper)
38% identity, 94% coverage: 9:422/440 of query aligns to 6:406/407 of 2nwwA

query
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2nwwA

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T

T

I

I

N

N

M

M

D

D

G

G

T
|
T

S

A

L

L

Y

Y

Q

Q

A

G

V

V

A

C

S

T

V

F

F

F

I

I

M

A

Q

N

V

A

L

L

W

G

P

S

E

H

G

-

L

L

T

T

F

V

S

G

N

Q

Q

Q

I

L

T

T

I

I

V

V

L

L

T

T

A

A

L

V

L

L

A

A

S

S

I

I

G

G

S

T

A

A

A

G

V

V

P

P

G

G

A
|
A

G
|
G

M

A

V

I

M

M

L

L

V

A

I

M

V

V

L

L

E

H

A

S

I

V

G

G

F

L

P

P

-

L

-

T

S

D

D

P

K

N

L

V

P

A

I

A

G

A

L

Y

A

A

L

M

I

I

F

L

A

G

V

I

D

D

R

A

P

I

L

L

D
|
D

M

M

L

G

R
|
R

T
|
T

T

M

V

V

N
|
N

V

V

T

T

G

G

D

D

A

L

T

T

V

G

S

T

M

A

I

I

V

V

A

A

K

K

S

T

binding (3s)-3-(benzyloxy)-l-aspartic acid: S269 (= S285), T305 (= T322), A349 (= A367), G350 (= G368), D385 (= D401), R388 (= R404), T389 (= T405), N392 (= N408)

6bavA Crystal structure of gltph r397c in complex with s-benzyl-l-cysteine (see paper)
38% identity, 95% coverage: 8:424/440 of query aligns to 6:409/409 of 6bavA

query
sites
6bavA

W

Q

K

K

I

I

M

L

I

I

G

G

M

L

I

I

L

L

G

G

I

A

L

I

F

V

G

G

F

L

L

I

M

L

T

G

T

H

L

Y

S

G

W

Y

G

A

K

D

G

A

F

V

T

K

G

-

D

T

W

Y

I

V

A

K

P

P

L

F

G

G

T

D

I

L

F

F

V

V

N

R

L

L

K

K

L

M

I

L

A

V

V

M

P

P

L

I

I

V

L

F

A

A

S

S

L

L

I

V

K

V

G

G

I

A

S

A

D

S

L

I

K

S

D

P

I

-

S

A

K

R

F

L

K

G

L

R

I

V

G

G

G

V

R

K

T

I

I

V

V

V

I

Y

Y

Y

I

L

L

L

T

T

T

S

V

A

I

F

A

A

I

V

T

T

I

L

G

G

L

I

L

M

V

A

N

R

T

L

F

F

K

N

P

P

G

G

N

A

G

G

I

I

T

-

P

-

E

-

T

-

I

-

E

-

K

-

L

-

T

-

E

-

Y

-

A

-

G

-

N

-

A

-

K

-

I

-

S

-

E

-

R

H

I

L

A

A

E

V

A

G

S

G

R

Q

Q

Q

-

F

-

Q

-

P

K

K

E

Q

S

A

G

P

P

P

L

L

-

V

Q

K

F

I

I

L

V

L

D

D

M

I

V

V

P

P

Q

T

N

N

A

P

F

F

K

G

A

A

M

L

S

A

D

-

N

N

K

G

M

Q

M

V

L

L

Q

P

V

T

I

I

F

F

A

A

I

I

F

I

L

L

G

G

I

I

S

A

M

I

L

T

L

Y

I

L

K

M

P

N

S

S

Q

E

S

N

E

E

P

K

L

V

K

R

K

K

-

S

-

A

-

E

-

T

F

L

D

D

S

A

L

I

N

N

-

G

-

L

-

A

D

E

V

A

V

M

L

Y

K

K

M

I

V

V

D

N

L

G

I

V

M

M

L

Q

T

Y

A

A

P

P

Y

I

A

G

V

V

F

F

A

A

L

L

L

I

A

A

N

-

V

Y

V

V

V

M

T

A

S

E

D

Q

D

G

P

V

D

K

I

V

L

V

L

G

A

E

L

L

L

A

K

K

Y

V

A

T

G

A

V

A

V

V

V

Y

F

V

G

G

L

L

A

T

L

L

M

Q

I

I

V

L

F

L

Y

V

C

Y

I

F

L

V

V

L

A

L

V

K

I

I

T

Y

K

G

K

I

S

D

P

P

L

I

W

S

F

F

L

I

K

K

E

K

I

A

S

K

P

D

A

A

Q

M

L

L

L

T

A

A

F

F

S

V

T

T

S
x
R

S

S

S
|
S

A

S

A

G

T

T

L

L

P

P

V

V

T

T

M

M

E

-

R

R

V

V

E

A

E

K

H

E

I

M

G

G

V

I

D

S

K

E

E

G

V

I

S

Y

S

S

F

F

V

T

L

L

P

P

V

L

G

G

A

A

T

T

I

I

N

N

M

M

D

D

G

G

T
|
T

S

A

L

L

Y
|
Y

Q

Q

A

G

V

V

A

C

S

T

V

F

F

F

I

I

M

A

Q

N

V

A

L

L

W

G

P

S

E

H

G

-

L

L

T

T

F

V

S

G

N

Q

Q

Q

I

L

T

T

I

I

V

V

L

L

T

T

A

A

L

V

L

L

A

A

S

S

I

I

G

G

S

T

A

A

A

G

V

V

P

P

G

G

A

A

G

G

M

A

V

I

M

M

L

L

V

A

I

M

V

V

L

L

E

E

A

S

I

V

G

G

F

L

P

P

-

L

-

T

S

D

D

P

K

N

L

V

P

A

I

A

G

A

L

Y

A

A

L

M

I

I

F

L

A

G

V

I

D
|
D

R

A

P

I

L

L

D
|
D

M

M

L

G

R
x
C

T
|
T

T

M

V

V

N

N

V

V

T

T

G

G

D

D

A

L

T

T

V

G

S

T

M

A

I

I

V

V

A

A

K

K

S

T

V

E

G

G

binding benzylcysteine: R268 (≠ S283), S270 (= S285), T306 (= T322), Y309 (= Y325), D382 (= D397), D386 (= D401), C389 (≠ R404), T390 (= T405)

6x14A Inward-facing state of the glutamate transporter homologue gltph in complex with tfb-tboa (see paper)
38% identity, 94% coverage: 9:422/440 of query aligns to 12:412/413 of 6x14A

query
sites
6x14A

K

K

I
|
I

M

L

I

I

G

G

M

L

I

I

L

L

G

G

I

A

L

I

F

V

G

G

F

L

L

I

M

L

T

G

T

H

L

Y

S

G

W

Y

G

A

K

H

G

A

F

V

T

H

G

-

D

T

W

Y

I

V

A

K

P

P

L

F

G

G

T

D

I

L

F

F

V

V

N

R

L

L

K

C

L

M

I

L

A

V

V

M

P

P

L

I

I

V

L

F

A

A

S

S

L

L

I

V

K

V

G
|
G

I

A

S

A

D

S

L

I

K

S

D

P

I

-

S

A

K

R

F

L

K

G

L

R

I

V

G

G

G

V

R

K

T

I

I
x
V

V

V

I

Y

Y

Y

I

L

L

L

T

T

T

S

V

A

I

F

A

A

I

V

T

T

I

L

G

G

L

I

L

M

V

A

N

R

T

L

F

F

K

N

P

P

G

G

N

A

G

G

I

I

T

-

P

-

E

-

T

-

I

-

E

-

K

-

L

-

T

-

E

-

Y

-

A

-

G

-

N

-

A

-

K

-

I

-

S

-

E

-

R

H

I

L

A

A

E

V

A

G

S

G

R

Q

Q

Q

-

F

-

Q

-

P

K

H

E

Q

S

A

G

P

P

P

L

L

-

V

Q

H

F

I

I

L

V

L

D

D

M

I

V

V

P

P

Q

T

N

N

A

P

F

F

K

G

A

A

M

L

S

A

D

-

N

N

K

G

M

Q

M

V

L

L

Q

P

V

T

I

I

F

F

A

A

I

I

F
x
I

L

L

G

G

I

I

S

A

M

I

L

T

L
x
Y

I

L

K

M

P

N

S

S

Q

E

S

N

E

E

P

K

L

V

K

R

K

K

-

S

-

A

-

E

-

T

F

L

D

D

S

A

L

I

N

N

-

G

-

L

-

A

D

E

V

A

V

M

L

Y

K
|
K

M

I

V

V

D

N

L

G

I

V

M
|
M

L

Q

T

Y

A

A

P

P

Y

I

A

G

V

V

F

F

A

A

L

L

L

I

A

A

N

-

V

Y

V

V

V

M

T

A

S

E

D

Q

D

G

P

V

D

H

I

V

L

V

L

G

A

E

L

L

L

A

K

K

Y

V

A

T

G

A

V

A

V

V

V

Y

F

V

G

G

L

L

A

T

L

L

M

Q

I

I

V

L

F

L

Y

V

C

Y

I

F

L

V

V

L

A

L

V

K

I

I

T

Y

K

G

K

I

S

D

P

P

L

I

W

S

F

F

L

I

K

K

E

H

I

A

S

K

P

D

A

A

Q

M

L

L

L

T

A

A

F

F

S

V

T

T

S

R

S

S

S
|
S

A

S

A

G

T

T

L

L

P

P

V

V

T

T

M

M

E

-

R

R

V

V

E

A

E

K

H

E

I

M

G

G

V

I

D

S

K

E

E

G

V

I

S

Y

S

S

F

F

V

T

L

L

P

P

V

L

G

G

A

A

T
|
T

I
|
I

N

N

M

M

D

D

G

G

T
|
T

S

A

L

L

Y

Y

Q

Q

A

G

V

V

A

A

S

T

V

F

F

F

I

I

M

A

Q

N

V

A

L

L

W

G

P

S

E

H

G

-

L

L

T

T

F

V

S

G

N

Q

Q

Q

I

L

T

T

I

I

V

V

L

L

T

T

A

A

L

V

L

L

A

A

S

S

I
|
I

G

G

S

T

A

A

A

G

V

V

P

P

G

G

A

A

G
|
G

M

A

V

I

M

M

L

L

V

C

I

M

V

V

L

L

E

H

A

S

I

V

G

G

F

L

P

P

-

L

-

T

S

D

D

P

K

N

L

V

P

A

I

A

G

A

L

Y

A

A

L

M

I

I

F
x
L

A

G

V

I

D

D

R

A

P

I

L

L

D
|
D

M

M

L

G

R
|
R

T

T

T

M

V

V

N

N

V

V

T

T

G

G

D

D

A

L

T

T

V

G

S

T

M

A

I

I

V

V

A

A

K

K

S

T

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: G66 (= G64), V83 (≠ I82), I157 (≠ F173), Y164 (≠ L180), K193 (= K202), T305 (= T316), I306 (= I317), I347 (= I359)
binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I13 (= I10), M199 (= M208), S275 (= S285), T311 (= T322), G356 (= G368), L384 (≠ F394), D391 (= D401), R394 (= R404)

Sites not aligning to the query:

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: 4

6bauA Crystal structure of gltph r397c in complex with l-cysteine (see paper)
38% identity, 94% coverage: 8:422/440 of query aligns to 6:407/408 of 6bauA

query
sites
6bauA

W

Q

K

K

I

I

M

L

I

I

G

G

M

L

I

I

L

L

G

G

I

A

L

I

F

V

G

G

F

L

L

I

M

L

T

G

T

H

L

Y

S

G

W

Y

G

A

K

D

G

A

F

V

T

K

G

-

D

T

W

Y

I

V

A

K

P

P

L

F

G

G

T

D

I

L

F

F

V

V

N

R

L

L

K

K

L

M

I

L

A

V

V

M

P

P

L

I

I

V

L

F

A

A

S

S

L

L

I

V

K

V

G

G

I

A

S

A

D

S

L

I

K

S

D

P

I

-

S

A

K

R

F

L

K

G

L

R

I

V

G

G

G

V

R

K

T

I

I

V

V

V

I

Y

Y

Y

I

L

L

L

T

T

T

S

V

A

I

F

A

A

I

V

T

T

I

L

G

G

L

I

L

M

V

A

N

R

T

L

F

F

K

N

P

P

G

G

N

A

G

G

I

I

T

-

P

-

E

-

T

-

I

-

E

-

K

-

L

-

T

-

E

-

Y

-

A

-

G

-

N

-

A

-

K

-

I

-

S

-

E

-

R

H

I

L

A

A

E

V

A

G

S

G

R

Q

Q

Q

-

F

-

Q

-

P

K

K

E

Q

S

A

G

P

P

P

L

L

-

V

Q

K

F

I

I

L

V

L

D

D

M

I

V

V

P

P

Q

T

N

N

A

P

F

F

K

G

A

A

M

L

S

A

D

-

N

N

K

G

M

Q

M

V

L

L

Q

P

V

T

I

I

F

F

A

A

I

I

F

I

L

L

G

G

I

I

S

A

M

I

L

T

L

Y

I

L

K

M

P

N

S

S

Q

E

S

N

E

E

P

K

L

V

K

R

K

K

-

S

-

A

-

E

-

T

F

L

D

D

S

A

L

I

N

N

-

G

-

L

-

A

D

E

V

A

V

M

L

Y

K

K

M

I

V

V

D

N

L

G

I

V

M

M

L

Q

T

Y

A

A

P

P

Y

I

A

G

V

V

F

F

A

A

L

L

L

I

A

A

N

-

V

Y

V

V

V

M

T

A

S

E

D

Q

D

G

P

V

D

K

I

V

L

V

L

G

A

E

L

L

L

A

K

K

Y

V

A

T

G

A

V

A

V

V

V

Y

F

V

G

G

L

L

A

T

L

L

M

Q

I

I

V

L

F

L

Y

V

C

Y

I

F

L

V

V

L

A

L

V

K

I

I

T

Y

K

G

K

I

S

D

P

P

L

I

W

S

F

F

L

I

K

K

E

K

I

A

S

K

P

D

A

A

Q

M

L

L

L

T

A

A

F

F

S

V

T

T

S

R

S

S

S
|
S

A

S

A

G

T

T

L

L

P

P

V

V

T

T

M

M

E

-

R

R

V

V

E

A

E

K

H

E

I

M

G

G

V

I

D

S

K

E

E

G

V

I

S

Y

S

S

F

F

V

T

L

L

P

P

V

L

G

G

A

A

T

T

I

I

N

N

M
|
M

D

D

G

G

T
|
T

S

A

L

L

Y

Y

Q

Q

A

G

V

V

A

C

S

T

V

F

F

F

I

I

M

A

Q

N

V

A

L

L

W

G

P

S

E

H

G

-

L

L

T

T

F

V

S

G

N

Q

Q

Q

I

L

T

T

I

I

V

V

L

L

T

T

A

A

L

V

L

L

A

A

S

S

I

I

G

G

S

T

A
|
A

A
x
G

V
|
V

P

P

G

G

A

A

G
|
G

M

A

V

I

M

M

L

L

V

A

I

M

V

V

L

L

E

E

A

S

I

V

G

G

F

L

P

P

-

L

-

T

S

D

D

P

K

N

L

V

P

A

I

A

G

A

L

Y

A

A

L

M

I

I

F

L

A

G

V

I

D

D

R

A

P

I

L

L

D
|
D

M

M

L

G

R
x
C

T
|
T

T

M

V

V

N
|
N

V

V

T

T

G

G

D

D

A

L

T

T

V

G

S

T

M

A

I

I

V

V

A

A

K

K

S

T

binding cysteine: S270 (= S285), M303 (= M319), T306 (= T322), A345 (= A362), G346 (≠ A363), V347 (= V364), G351 (= G368), D386 (= D401), C389 (≠ R404), T390 (= T405), N393 (= N408)

6zgbA Glutamate transporter homologue glttk in complex with a photo cage compound (see paper)
36% identity, 96% coverage: 8:428/440 of query aligns to 10:419/425 of 6zgbA

query
sites
6zgbA

W

W

K

K

I

I

M

L

I

W

G

G

M

L

I

V

L

L

G

G

I

A

L

V

F

F

G

G

F

L

L

I

M

A

T

G

T

H

L

F

S

G

W

Y

G

-

K

A

G

G

F

A

T

V

G

K

D

T

W

Y

I

I

A

K

P

P

L

F

G

G

T

D

I

L

F

F

V

V

N

R

L

L

K

K

L

M

I

L

A

V

V

M

P

P

L

I

I

V

L

L

A

A

S

S

L

L

I

V

K

V

G

G

I

A

S

A

D

S

L

I

K

S

D

P

I

-

S

A

K

R

F

L

K

G

L

R

I

V

G

G

G

V

R

K

T

I

I

V

V

V

I

Y

Y

Y

I

L

L

A

T

T

T

S

V

A

I

M

A

A

I

V

T

F

I

F

G

G

L

L

L

I

L

V

V

G

N

R

T

L

F

F

K

N

P

V

G

G

N

-

G

-

I

-

T

-

P

-

E

-

T

-

I

-

E

-

K

-

L

-

T

-

E

-

Y

-

A

-

G

A

N

N

A

V

K

N

I

L

S

G

E

S

R

G

I

T

A

G

E

K

A

A

S

I

R

E

Q

A

K

Q

E

P

S

P

G

S

P

L

L

V

Q

Q

F

T

I

L

V

L

D

N

M

I

V

V

P

P

Q

T

N

N

A

P

F

F

K

A

A

S

M

L

S

A

D

K

N

G

K

E

M

V

M

-

L

L

Q

P

V

V

I

I

F

F

A

A

I

I

F

I

L

L

G

G

I

I

S

A

M

I

L

T

L

Y

I

L

K

M

P

N

S

R

Q

N

S

E

E

E

P

R

L

V

K

R

K

K

-

S

-

A

-

E

-

T

-

L

-

R

F

V

F

F

D

D

S

G

L

L

N

A

D

E

V

A

V

M

L

Y

K

L

M

I

V

V

D

G

L

G

I

V

M

M

L

Q

T

Y

A

A

P

P

Y

I

A

G

V

V

F

F

A

A

L

L

L

I

A

A

N

Y

V

V

V

M

V

-

T

A

S

E

D

Q

D

G

P

V

D

R

I

V

L

V

L

G

A

P

L

L

L

A

K

K

Y

V

A

V

G

G

V

A

V

V

V

Y

F

T

G

G

L

L

A

F

L

L

M

Q

I

I

V

V

F

I

Y

T

C

Y

I

F

L

I

V

L

A

L

V

K

I

V

T

F

K

G

K

I

S

D

P

P

L

I

W

K

F

F

L

I

K

R

E

K

I

A

S

K

P

D

A

A

Q

M

L

I

L

T

A

A

F

F

S

V

T

T

S
x
R

S
|
S

A

S

A

G

T

T

L

L

P

P

V

V

T

T

M

M

E

R

R

V

V

A

E

E

H

E

I

M

G

G

V

V

D

D

K

K

E

G

V

I

S

F

S

S

F

F

V

T

L

L

P

P

V

L

G

G

A

A

T

T

I

I

N

N

M
|
M

D

D

G

G

T

T

S

A

L

L

Y

Y

Q

Q

A

G

V

V

A

T

S

V

V

L

F

F

I

V

M

A

Q

N

V

A

L

I

W

G

P

-

E

H

G

P

L

L

T

T

F

L

S

G

N

Q

Q

Q

I

L

T

V

I

V

V

V

L

L

T

T

A

A

L

V

L

L

A

A

S

S

I

I

G

G

S

T

A

A

A

G

V

V

P

P

G

G

A

A

G

G

M

A

V

I

M

M

L

L

V

A

I

M

V

V

L

L

E

Q

A

S

I

V

G

G

F

L

-

D

-

L

-

T

P

P

S

G

D

S

K

P

L

V

P

A

I

L

G

A

L

Y

A

A

L

M

I

I

F

L

A

G

V

I

D

D

R

A

P

I

L

L

D
|
D

M

M

L

G

R
|
R

T
|
T

T

M

V

V

N

N

V

V

T

T

G

G

D

D

A

L

T

A

V

G

S

T

M

V

I

I

V

V

A

A

K

K

S

T

V

E

G

K

K

E

L

L

G

D

E

E

binding (2~{S},3~{S})-2-azanyl-3-[(2-nitrophenyl)methoxy]butanedioic acid: R272 (≠ S283), S273 (= S284), S274 (= S285), M308 (= M319), D392 (= D401), R395 (= R404), T396 (= T405)

6zl4A The structure of glutamate transporter homologue glttk in complex with the photo switchable compound (cis) (see paper)
36% identity, 96% coverage: 8:428/440 of query aligns to 9:418/424 of 6zl4A

query
sites
6zl4A

W

W

K

K

I

I

M

L

I

W

G

G

M

L

I

V

L

L

G

G

I

A

L

V

F

F

G

G

F

L

L

I

M

A

T

G

T

H

L

F

S

G

W

Y

G

-

K

A

G

G

F

A

T

V

G

K

D

T

W

Y

I

I

A

K

P

P

L

F

G

G

T

D

I

L

F

F

V

V

N

R

L

L

K

K

L

M

I

L

A

V

V

M

P

P

L

I

I

V

L

L

A

A

S

S

L

L

I

V

K

V

G

G

I

A

S

A

D

S

L

I

K

S

D

P

I

-

S

A

K

R

F

L

K

G

L

R

I

V

G

G

G

V

R

K

T

I

I

V

V

V

I

Y

Y

Y

I

L

L

A

T

T

T

S

V

A

I

M

A

A

I

V

T

F

I

F

G

G

L

L

L

I

L

V

V

G

N

R

T

L

F

F

K

N

P

V

G

G

N

-

G

-

I

-

T

-

P

-

E

-

T

-

I

-

E

-

K

-

L

-

T

-

E

-

Y

-

A

-

G

A

N

N

A

V

K

N

I

L

S

G

E

S

R

G

I

T

A

G

E

K

A

A

S

I

R

E

Q

A

K

Q

E

P

S

P

G

S

P

L

L

V

Q

Q

F

T

I

L

V

L

D

N

M

I

V

V

P

P

Q

T

N

N

A

P

F

F

K

A

A

S

M

L

S

A

D

K

N

G

K

E

M

V

M

-

L

L

Q

P

V

V

I

I

F

F

A

A

I

I

F

I

L

L

G

G

I

I

S

A

M

I

L

T

L

Y

I

L

K

M

P

N

S

R

Q

N

S

E

E

E

P

R

L

V

K

R

K

K

-

S

-

A

-

E

-

T

-

L

-

R

F

V

F

F

D

D

S

G

L

L

N

A

D

E

V

A

V

M

L

Y

K
x
L

M

I

V

V

D

G

L
x
G

I

V

M

M

L

Q

T

Y

A

A

P

P

Y

I

A

G

V

V

F

F

A

A

L

L

L

I

A

A

N

Y

V

V

V

M

V

-

T

A

S

E

D

Q

D

G

P

V

D

R

I

V

L

V

L

G

A

P

L

L

L

A

K

K

Y

V

A

V

G

G

V

A

V

V

V

Y

F

T

G

G

L

L

A

F

L

L

M

Q

I

I

V

V

F

I

Y

T

C

Y

I

F

L

I

V

L

A

L

V

K

I

V

T

F

K

G

K

I

S

D

P

P

L

I

W

K

F

F

L

I

K

R

E

K

I

A

S

K

P

D

A

A

Q

M

L

I

L

T

A

A

F

F

S

V

T

T

S
x
R

S
|
S

A

S

A

G

T

T

L

L

P

P

V

V

T

T

M

M

E
x
R

R

V

V

A

E

E

H

E

I

M

G

G

V

V

D

D

K

K

E

G

V

I

S

F

S

S

F

F

V

T

L

L

P

P

V

L

G

G

A

A

T

T

I

I

N

N

M
|
M

D

D

G

G

T
|
T

S

A

L

L

Y

Y

Q

Q

A

G

V

V

A

T

S

V

V

L

F

F

I

V

M

A

Q

N

V

A

L

I

W

G

P

-

E

H

G

P

L

L

T

T

F

L

S

G

N

Q

Q

Q

I

L

T

V

I

V

V

V

L

L

T

T

A

A

L

V

L

L

A

A

S

S

I

I

G

G

S

T

A

A

A

G

V

V

P

P

G
|
G

A
|
A

G

G

M

A

V

I

M

M

L

L

V

A

I

M

V

V

L

L

E

Q

A

S

I

V

G

G

F

L

-

D

-

L

-

T

P

P

S

G

D

S

K

P

L

V

P

A

I

L

G

A

L

Y

A

A

L

M

I

I

F

L

A

G

V

I

D

D

R

A

P

I

L

L

D

D

M

M

L

G

R
|
R

T
|
T

T

M

V

V

N

N

V

V

T

T

G

G

D

D

A

L

T

A

V

G

S

T

M

V

I

I

V

V

A

A

K

K

S

T

V

E

G

K

K

E

L

L

G

D

E

E

binding decyl-beta-d-maltopyranoside: L191 (≠ K202), G195 (≠ L206), R282 (≠ E294)
binding (2~{S},3~{S})-2-azanyl-3-[[4-[2-(4-methoxyphenyl)hydrazinyl]phenyl]methoxy]butanedioic acid: R271 (≠ S283), S272 (= S284), S273 (= S285), M307 (= M319), T310 (= T322), G353 (= G366), A354 (= A367), R394 (= R404), T395 (= T405)

Sites not aligning to the query:

binding decyl-beta-d-maltopyranoside: 3

5e9sA Crystal structure of substrate-bound glutamate transporter homologue glttk (see paper)
36% identity, 96% coverage: 8:428/440 of query aligns to 12:421/427 of 5e9sA

query
sites
5e9sA

W

W

K

K

I

I

M

L

I

W

G

G

M

L

I

V

L

L

G

G

I

A

L

V

F

F

G

G

F

L

L

I

M

A

T

G

T

H

L

F

S

G

W

Y

G

-

K

A

G

G

F

A

T

V

G

K

D

T

W

Y

I

I

A

K

P

P

L

F

G

G

T

D

I

L

F

F

V

V

N

R

L

L

K

K

L

M

I

L

A

V

V

M

P

P

L

I

I

V

L

L

A

A

S

S

L

L

I

V

K

V

G

G

I

A

S

A

D

S

L

I

K

S

D

P

I

-

S

A

K

R

F

L

K

G

L

R

I

V

G

G

G

V

R

K

T

I

I

V

V

V

I

Y

Y
|
Y

I

L

L

A

T
|
T

T
x
S

V

A

I

M

A

A

I

V

T

F

I

F

G

G

L

L

L

I

L

V

V

G

N

R

T

L

F

F

K

N

P

V

G

G

N

-

G

-

I

-

T

-

P

-

E

-

T

-

I

-

E

-

K

-

L

-

T

-

E

-

Y

-

A

-

G

A

N

N

A

V

K

N

I

L

S

G

E

S

R

G

I

T

A

G

E

K

A

A

S

I

R

E

Q

A

K

Q

E

P

S

P

G

S

P

L

L

V

Q

Q

F

T

I

L

V

L

D

N

M

I

V

V

P

P

Q

T

N

N

A

P

F

F

K

A

A

S

M

L

S

A

D

K

N

G

K

E

M

V

M

-

L

L

Q

P

V

V

I

I

F

F

A

A

I

I

F

I

L

L

G

G

I

I

S

A

M

I

L

T

L

Y

I

L

K

M

P

N

S

R

Q

N

S

E

E

E

P

R

L

V

K

R

K

K

-

S

-

A

-

E

-

T

-

L

-

R

F

V

F

F

D

D

S

G

L

L

N

A

D

E

V

A

V

M

L

Y

K
x
L

M

I

V

V

D

G

L
x
G

I

V

M

M

L

Q

T
x
Y

A

A

P

P

Y

I

A

G

V

V

F

F

A

A

L

L

L

I

A

A

N

Y

V

V

V

M

V

-

T

A

S

E

D

Q

D

G

P

V

D

R

I

V

L

V

L

G

A

P

L

L

L

A

K

K

Y

V

A

V

G

G

V

A

V

V

V

Y

F

T

G

G

L

L

A

F

L

L

M

Q

I

I

V

V

F

I

Y

T

C

Y

I

F

L

I

V

L

A

L

V

K

I

V

T

F

K

G

K

I

S

D

P

P

L

I

W

K

F

F

L

I

K

R

E

K

I

A

S

K

P

D

A

A

Q

M

L

I

L

T

A

A

F

F

S

V

T

T

S
x
R

S
|
S

A

S

A

G

T

T

L

L

P

P

V

V

T

T

M

M

E

R

R

V

V

A

E

E

H

E

I

M

G

G

V

V

D

D

K

K

E

G

V

I

S

F

S

S

F

F

V

T

L

L

P

P

V

L

G
|
G

A
|
A

T
|
T

I

I

N
|
N

M
|
M

D
|
D

G

G

T
|
T

S

A

L

L

Y

Y

Q

Q

A

G

V

V

A

T

S

V

V

L

F

F

I

V

M

A

Q

N

V

A

L

I

W

G

P

-

E

H

G

P

L

L

T

T

F

L

S

G

N

Q

Q

Q

I

L

T

V

I

V

V

V

L

L

T

T

A

A

L

V

L

L

A

A

S
|
S

I
|
I

G
|
G

S
x
T

A

A

A
x
G

V
|
V

P

P

G

G

A
|
A

G
|
G

M

A

V

I

M

M

L

L

V

A

I

M

V

V

L

L

E

Q

A

S

I

V

G

G

F

L

-

D

-

L

-

T

P

P

S

G

D

S

K

P

L

V

P

A

I

L

G

A

L

Y

A

A

L

M

I

I

F

L

A

G

V

I

D

D

R

A

P

I

L

L

D
|
D

M

M

L

G

R
|
R

T
|
T

T

M

V

V

N
|
N

V
|
V

T

T

G

G

D
|
D

A

L

T

A

V

G

S

T

M

V

I

I

V

V

A

A

K

K

S

T

V

E

G

K

K

E

L

L

G

D

E

E

binding aspartic acid: R274 (≠ S283), S275 (= S284), S276 (= S285), T313 (= T322), G353 (≠ A363), V354 (= V364), A357 (= A367), G358 (= G368), D394 (= D401), R397 (= R404), T398 (= T405)
binding decyl-beta-d-maltopyranoside: L194 (≠ K202), G198 (≠ L206), Y202 (≠ T210)
binding sodium ion: Y87 (= Y85), T90 (= T88), S91 (≠ T89), S276 (= S285), G305 (= G314), A306 (= A315), T307 (= T316), N309 (= N318), N309 (= N318), M310 (= M319), D311 (= D320), S348 (= S358), I349 (= I359), G350 (= G360), T351 (≠ S361), N401 (= N408), V402 (= V409), D405 (= D412)

6xwnB Structure of glutamate transporter homologue glttk in the presence of tboa inhibitor (see paper)
36% identity, 96% coverage: 8:428/440 of query aligns to 12:421/426 of 6xwnB

query
sites
6xwnB

W

W

K

K

I

I

M

L

I

W

G

G

M

L

I

V

L

L

G

G

I

A

L

V

F

F

G

G

F

L

L

I

M

A

T

G

T

H

L

F

S

G

W

Y

G

-

K

A

G

G

F

A

T

V

G

K

D

T

W

Y

I

I

A

K

P

P

L

F

G

G

T

D

I

L

F

F

V

V

N

R

L

L

K

K

L

M

I

L

A

V

V

M

P

P

L

I

I

V

L

L

A

A

S

S

L

L

I

V

K

V

G

G

I

A

S

A

D

S

L

I

K

S

D

P

I

-

S

A

K

R

F

L

K

G

L

R

I

V

G

G

G

V

R

K

T

I

I

V

V

V

I

Y

Y

Y

I

L

L

A

T

T

T

S

V

A

I

M

A

A

I

V

T

F

I

F

G

G

L

L

L

I

L

V

V

G

N

R

T

L

F

F

K

N

P

V

G

G

N

-

G

-

I

-

T

-

P

-

E

-

T

-

I

-

E

-

K

-

L

-

T

-

E

-

Y

-

A

-

G

A

N

N

A

V

K

N

I

L

S

G

E

S

R

G

I

T

A

G

E

K

A

A

S

I

R

E

Q

A

K

Q

E

P

S

P

G

S

P

L

L

V

Q

Q

F

T

I

L

V

L

D

N

M

I

V

V

P

P

Q

T

N

N

A

P

F

F

K

A

A

S

M

L

S

A

D

K

N

G

K

E

M

V

M

-

L

L

Q

P

V

V

I

I

F

F

A

A

I

I

F

I

L

L

G

G

I

I

S

A

M

I

L

T

L

Y

I

L

K

M

P

N

S

R

Q

N

S

E

E

E

P

R

L

V

K

R

K

K

-

S

-

A

-

E

-

T

-

L

-

R

F

V

F

F

D

D

S

G

L

L

N

A

D

E

V

A

V

M

L

Y

K

L

M

I

V

V

D

G

L

G

I

V

M

M

L

Q

T

Y

A

A

P

P

Y

I

A

G

V

V

F

F

A

A

L

L

L

I

A

A

N

Y

V

V

V

M

V

-

T

A

S

E

D

Q

D

G

P

V

D

R

I

V

L

V

L

G

A

P

L

L

L

A

K

K

Y

V

A

V

G

G

V

A

V

V

V

Y

F

T

G

G

L

L

A

F

L

L

M

Q

I

I

V

V

F

I

Y

T

C

Y

I

F

L

I

V

L

A

L

V

K

I

V

T

F

K

G

K

I

S

D

P

P

L

I

W

K

F

F

L

I

K

R

E

K

I

A

S

K

P

D

A

A

Q

M

L

I

L

T

A

A

F

F

S

V

T

T

S

R

S
|
S

A

S

A

G

T

T

L

L

P

P

V

V

T

T

M

M

E

R

R

V

V

A

E

E

H

E

I

M

G

G

V

V

D

D

K

K

E

G

V

I

S

F

S

S

F

F

V

T

L

L

P

P

V

L

G

G

A

A

T

T

I

I

N

N

M
|
M

D

D

G

G

T
|
T

S

A

L

L

Y

Y

Q

Q

A

G

V

V

A

T

S

V

V

L

F

F

I

V

M

A

Q

N

V

A

L

I

W

G

P

-

E

H

G

P

L

L

T

T

F

L

S

G

N

Q

Q

Q

I

L

T

V

I

V

V

V

L

L

T

T

A

A

L

V

L

L

A

A

S

S

I

I

G

G

S

T

A

A

A

G

V

V

P

P

G

G

A

A

G

G

M

A

V

I

M

M

L

L

V

A

I

M

V

V

L

L

E

Q

A

S

I

V

G

G

F

L

-

D

-

L

-

T

P

P

S

G

D

S

K

P

L

V

P

A

I

L

G

A

L

Y

A

A

L

M

I

I

F

L

A

G

V

I

D

D

R

A

P

I

L

L

D
|
D

M

M

L

G

R
|
R

T

T

T

M

V

V

N

N

V

V

T

T

G

G

D

D

A

L

T

A

V

G

S

T

M

V

I

I

V

V

A

A

K

K

S

T

V

E

G

K

K

E

L

L

G

D

E

E

binding (3s)-3-(benzyloxy)-l-aspartic acid: S275 (= S284), S276 (= S285), M310 (= M319), T313 (= T322), D394 (= D401), R397 (= R404)

6r7rA Crystal structure of the glutamate transporter homologue glttk in complex with d-aspartate (see paper)
35% identity, 96% coverage: 8:428/440 of query aligns to 5:410/416 of 6r7rA

query
sites
6r7rA

W

W

K

K

I

I

M

L

I

W

G

G

M

L

I

V

L

L

G

G

I

A

L

V

F

F

G

G

F

L

L

I

M

A

T

G

T
x
H

L

F

S

G

W

Y

G

-

K

A

G

G

F

A

T

V

G

K

D

T

W

Y

I

I

A

K

P

P

L

F

G

G

T

D

I

L

F

F

V

V

N

R

L

L

K

K

L

M

I

L

A

V

V

M

P

P

L

I

I

V

L

L

A

A

S

S

L

L

I

V

K

V

G

G

I

A

S

A

D

S

L

I

K

S

D

P

I

-

S

A

K

R

F

L

K

G

L

R

I

V

G

G

G

V

R

K

T

I

I

V

V

V

I

Y

Y

Y

I

L

L

A

T

T

T

S

V

A

I

M

A

A

I

V

T

F

I

F

G

G

L

L

L

I

L

V

V

G

N

R

T

L

F

F

K

N

P

V

G

G

N

A

G

N

I

V

T

-

P

-

E

-

T

-

I

-

E

-

K

-

L

-

T

-

E

-

Y

-

A

-

G

-

N

-

A

-

K

-

I

-

S

-

E

-

R

N

I

L

A

G

E

S

A

G

S

T

R

G

Q

A

K

Q

E

P

S

P

G

S

P

L

L

V

Q

Q

F

T

I

L

V

L

D

N

M

I

V

V

P

P

Q

T

N

N

A

P

F

F

K

A

A

S

M

L

S

A

D

K

N

G

K

E

M

V

M

-

L

L

Q

P

V

V

I

I

F

F

A

A

I

I

F

I

L

L

G

G

I

I

S

A

M

I

L

T

L

Y

I

L

K

M

P

N

S

R

Q

N

S

E

E

E

P

R

L

V

K

R

K

K

-

S

-

A

-

E

-

T

-

L

-

R

F

V

F

F

D

D

S

G

L

L

N

A

D

E

V

A

V

M

L

Y

K

L

M

I

V

V

D

G

L

G

I

V

M

M

L

Q

T

Y

A

A

P

P

Y

I

A

G

V

V

F

F

A

A

L

L

L

I

A

A

N

Y

V

V

V

M

V

-

T

A

S

E

D

Q

D

G

P

V

D

R

I

V