SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_024771162.1 NCBI__GCF_000520995.1:WP_024771162.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P07821 Iron(3+)-hydroxamate import ATP-binding protein FhuC; Ferric hydroxamate uptake protein C; Ferrichrome transport ATP-binding protein FhuC; Iron(III)-hydroxamate import ATP-binding protein FhuC; EC 7.2.2.16 from Escherichia coli (strain K12) (see 2 papers)
32% identity, 88% coverage: 32:262/264 of query aligns to 30:261/265 of P07821

query
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P07821

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K50 (= K52) mutation to Q: Lack of activity.
D172 (= D174) mutation to E: Lack of activity.
E173 (= E175) mutation to A: Lack of activity.

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
31% identity, 78% coverage: 32:236/264 of query aligns to 21:223/241 of 4u00A

query
sites
4u00A

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binding adenosine-5'-diphosphate: S37 (≠ N48), G38 (= G49), S39 (≠ I50), G40 (= G51), K41 (= K52), S42 (= S53), T43 (= T54)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12, 17

1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
28% identity, 81% coverage: 27:241/264 of query aligns to 14:223/367 of 1q12A

query
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1q12A

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binding adenosine-5'-triphosphate: S35 (≠ N48), G36 (= G49), C37 (≠ I50), G38 (= G51), K39 (= K52), S40 (= S53), T41 (= T54), R126 (≠ K145), A130 (≠ E149), S132 (= S151), G134 (= G153), Q135 (= Q154)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 10

P9WQK5 Uncharacterized ABC transporter ATP-binding protein Rv0073 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
30% identity, 81% coverage: 32:246/264 of query aligns to 26:238/330 of P9WQK5

query
sites
P9WQK5

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K65 (≠ M71) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
28% identity, 81% coverage: 27:241/264 of query aligns to 17:226/371 of P68187

query
sites
P68187

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A85 (≠ I97) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (≠ M122) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (≠ I130) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (≠ A133) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (= E135) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (≠ G140) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G153) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D174) mutation to N: Loss of maltose transport but retains ability to repress mal genes.

Sites not aligning to the query:

228 R→C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
267 W→G: Normal maltose transport but constitutive mal gene expression.
278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
28% identity, 81% coverage: 27:241/264 of query aligns to 16:225/371 of 3puyA

query
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3puyA

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K
|
K

S
|
S

T
|
T

L

L

R

R

T

M

L

I

S

A

R

G

V

L

Q

E

P

T

A

I

L

T

H

S

G

G

K

D

I

L

M

F

L

I

S

G

D

E

I

K

E

R

L

M

Q

N

S

D

Y

T

K

P

S

P

V

A

E

E

L

R

A

G

S

-

Q

-

L

V

S

G

I

M

V

V

L

F

T
x
Q

E

S

Q

Y

I

A

A

L

S

Y

K

P

N

H

L

L

T

S

V

V

Q

A

E

E

L

N

V

M

A

S

L

F

G

G

R

-

Q

-

P

-

Y

-

T

L

N

K

W

L

V

A

G

G

K

A

M

K

A

K

E

E

E

V

D

I

I

N

E

Q

Q

R

I

V

R

N

K

Q

A

V

I

A

E

E

V

V

T

L

H

Q

I

L

G

A

D

H

L

L

V

L

A

D

K
x
R

R

K

C

P

F

K

E
x
A

L

L

S
|
S

D

G

G
|
G

Q
|
Q

L

R

Q

Q

K

R

V

V

L

A

I

I

A

G

R

R

A

T

I

L

A

V

Q

A

D

E

T

P

P

S

F

V

I

F

M

L

L

L

D

D

E
|
E

P

P

T

L

T

S

H

N

L

L

D

D

V

A

Y

A

H

L

K

R

A

V

Y

Q

I

M

L

R

K

I

L

E

L

I

K

S

R

R

L

L

A

H

F

K

E

R

T

L

R

G

K

R

T

T

I

M

L

I

F

Y

S

V

T

T

H
|
H

E

D

I

Q

D

V

F

E

A

A

I

M

Q

T

L

L

C

A

D

D

K

K

M

I

I

V

V

V

M

L

N

D

E

A

D

G

G

R

F

V

-

A

E

Q

F

V

G

G

R

K

P

P

K

L

E

E

L

L

V

Y

Q

H

C

Y

R

P

A

A

binding phosphoaminophosphonic acid-adenylate ester: S37 (≠ N48), G38 (= G49), C39 (≠ I50), G40 (= G51), K41 (= K52), S42 (= S53), T43 (= T54), Q81 (≠ T94), R128 (≠ K145), A132 (≠ E149), S134 (= S151), G136 (= G153), Q137 (= Q154), E158 (= E175), H191 (= H208)
binding magnesium ion: S42 (= S53), Q81 (≠ T94)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 12

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
28% identity, 81% coverage: 27:241/264 of query aligns to 16:225/371 of 3puxA

query
sites
3puxA

V

V

I

V

A

S

A

K

D

D

I

I

N

N

L

L

K

D

L

I

H

H

E

E

G

G

E

E

L

F

V

V

G

V

L

F

V

V

G

G

A

P

N
x
S

G
|
G

I
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

R

T

M

L

I

S

A

R

G

V

L

Q

E

P

T

A

I

L

T

H

S

G

G

K

D

I

L

M

F

L

I

S

G

D

E

I

K

E

R

L

M

Q

N

S

D

Y

T

K

P

S

P

V

A

E

E

L

R

A

G

S

-

Q

-

L

V

S

G

I

M

V

V

L

F

T
x
Q

E

S

Q

Y

I

A

A

L

S

Y

K

P

N

H

L

L

T

S

V

V

Q

A

E

E

L

N

V

M

A

S

L

F

G

G

R

-

Q

-

P

-

Y

-

T

L

N

K

W

L

V

A

G

G

K

A

M

K

A

K

E

E

E

V

D

I

I

N

E

Q

Q

R

I

V

R

N

K

Q

A

V

I

A

E

E

V

V

T

L

H

Q

I

L

G

A

D

H

L

L

V

L

A

D

K
x
R

R

K

C

P

F

K

E

A

L

L

S
|
S

D

G

G
|
G

Q
|
Q

L

R

Q

Q

K

R

V

V

L

A

I

I

A

G

R

R

A

T

I

L

A

V

Q

A

D

E

T

P

P

S

F

V

I

F

M

L

L

L

D

D

E

E

P

P

T

L

T

S

H

N

L

L

D

D

V

A

Y

A

H

L

K

R

A

V

Y

Q

I

M

L

R

K

I

L

E

L

I

K

S

R

R

L

L

A

H

F

K

E

R

T

L

R

G

K

R

T

T

I

M

L

I

F

Y

S

V

T

T

H
|
H

E

D

I

Q

D

V

F

E

A

A

I

M

Q

T

L

L

C

A

D

D

K

K

M

I

I

V

V

V

M

L

N

D

E

A

D

G

G

R

F

V

-

A

E

Q

F

V

G

G

R

K

P

P

K

L

E

E

L

L

V

Y

Q

H

C

Y

R

P

A

A

binding adenosine-5'-diphosphate: G38 (= G49), C39 (≠ I50), G40 (= G51), K41 (= K52), S42 (= S53), T43 (= T54), R128 (≠ K145), S134 (= S151), Q137 (= Q154)
binding beryllium trifluoride ion: S37 (≠ N48), G38 (= G49), K41 (= K52), Q81 (≠ T94), S134 (= S151), G136 (= G153), H191 (= H208)
binding magnesium ion: S42 (= S53), Q81 (≠ T94)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12

3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
28% identity, 81% coverage: 27:241/264 of query aligns to 16:225/371 of 3puwA

query
sites
3puwA

V

V

I
x
V

A

S

A

K

D

D

I

I

N

N

L

L

K

D

L

I

H

H

E

E

G

G

E

E

L

F

V

V

G

V

L

F

V

V

G

G

A

P

N
x
S

G
|
G

I
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

R

T

M

L

I

S

A

R

G

V

L

Q

E

P

T

A

I

L

T

H

S

G

G

K

D

I

L

M

F

L

I

S

G

D

E

I

K

E

R

L

M

Q

N

S

D

Y

T

K

P

S

P

V

A

E

E

L

R

A

G

S

-

Q

-

L

V

S

G

I

M

V

V

L

F

T
x
Q

E

S

Q

Y

I

A

A

L

S

Y

K

P

N

H

L

L

T

S

V

V

Q

A

E

E

L

N

V

M

A

S

L

F

G

G

R

-

Q

-

P

-

Y

-

T

L

N

K

W

L

V

A

G

G

K

A

M

K

A

K

E

E

E

V

D

I

I

N

E

Q

Q

R

I

V

R

N

K

Q

A

V

I

A

E

E

V

V

T

L

H

Q

I

L

G

A

D

H

L

L

V

L

A

D

K
x
R

R

K

C

P

F

K

E
x
A

L

L

S
|
S

D
x
G

G
|
G

Q
|
Q

L

R

Q

Q

K

R

V

V

L

A

I

I

A

G

R

R

A

T

I

L

A

V

Q

A

D

E

T

P

P

S

F

V

I

F

M

L

L

L

D

D

E
|
E

P

P

T

L

T

S

H

N

L

L

D

D

V

A

Y

A

H

L

K

R

A

V

Y

Q

I

M

L

R

K

I

L

E

L

I

K

S

R

R

L

L

A

H

F

K

E

R

T

L

R

G

K

R

T

T

I

M

L

I

F

Y

S

V

T

T

H
|
H

E

D

I

Q

D

V

F

E

A

A

I

M

Q

T

L

L

C

A

D

D

K

K

M

I

I

V

V

V

M

L

N

D

E

A

D

G

G

R

F

V

-

A

E

Q

F

V

G

G

R

K

P

P

K

L

E

E

L

L

V

Y

Q

H

C

Y

R

P

A

A

binding adenosine-5'-diphosphate: V17 (≠ I28), G38 (= G49), C39 (≠ I50), G40 (= G51), K41 (= K52), S42 (= S53), T43 (= T54), R128 (≠ K145), A132 (≠ E149), S134 (= S151), Q137 (= Q154)
binding tetrafluoroaluminate ion: S37 (≠ N48), G38 (= G49), K41 (= K52), Q81 (≠ T94), S134 (= S151), G135 (≠ D152), G136 (= G153), E158 (= E175), H191 (= H208)
binding magnesium ion: S42 (= S53), Q81 (≠ T94)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12

3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
28% identity, 81% coverage: 27:241/264 of query aligns to 16:225/371 of 3puvA

query
sites
3puvA

V

V

I
x
V

A

S

A

K

D

D

I

I

N

N

L

L

K

D

L

I

H

H

E

E

G

G

E

E

L

F

V

V

G

V

L

F

V

V

G

G

A

P

N

S

G
|
G

I
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

R

T

M

L

I

S

A

R

G

V

L

Q

E

P

T

A

I

L

T

H

S

G

G

K

D

I

L

M

F

L

I

S

G

D

E

I

K

E

R

L

M

Q

N

S

D

Y

T

K

P

S

P

V

A

E

E

L

R

A

G

S

-

Q

-

L

V

S

G

I

M

V

V

L

F

T
x
Q

E

S

Q

Y

I

A

A

L

S

Y

K

P

N

H

L

L

T

S

V

V

Q

A

E

E

L

N

V

M

A

S

L

F

G

G

R

-

Q

-

P

-

Y

-

T

L

N

K

W

L

V

A

G

G

K

A

M

K

A

K

E

E

E

V

D

I

I

N

E

Q

Q

R

I

V

R

N

K

Q

A

V

I

A

E

E

V

V

T

L

H

Q

I

L

G

A

D

H

L

L

V

L

A

D

K
x
R

R

K

C

P

F

K

E
x
A

L

L

S
|
S

D

G

G

G

Q
|
Q

L

R

Q

Q

K

R

V

V

L

A

I

I

A

G

R

R

A

T

I

L

A

V

Q

A

D

E

T

P

P

S

F

V

I

F

M

L

L

L

D

D

E

E

P

P

T

L

T

S

H

N

L

L

D

D

V

A

Y

A

H

L

K

R

A

V

Y

Q

I

M

L

R

K

I

L

E

L

I

K

S

R

R

L

L

A

H

F

K

E

R

T

L

R

G

K

R

T

T

I

M

L

I

F

Y

S

V

T

T

H

H

E

D

I

Q

D

V

F

E

A

A

I

M

Q

T

L

L

C

A

D

D

K

K

M

I

I

V

V

V

M

L

N

D

E

A

D

G

G

R

F

V

-

A

E

Q

F

V

G

G

R

K

P

P

K

L

E

E

L

L

V

Y

Q

H

C

Y

R

P

A

A

binding adenosine-5'-diphosphate: V17 (≠ I28), G38 (= G49), C39 (≠ I50), G40 (= G51), K41 (= K52), S42 (= S53), T43 (= T54), R128 (≠ K145), A132 (≠ E149), S134 (= S151), Q137 (= Q154)
binding magnesium ion: S42 (= S53), Q81 (≠ T94)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12

2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
28% identity, 80% coverage: 27:236/264 of query aligns to 16:220/374 of 2awnB

query
sites
2awnB

V

V

I
x
V

A

S

A

K

D

D

I

I

N

N

L

L

K

D

L

I

H

H

E

E

G

G

E

E

L

F

V

V

G

V

L

F

V

V

G

G

A

P

N
x
S

G
|
G

I

C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

R

T

M

L

I

S

A

R

G

V

L

Q

E

P

T

A

I

L

T

H

S

G

G

K

D

I

L

M

F

L

I

S

G

D

E

I

K

E

R

L

M

Q

N

S

D

Y

T

K

P

S

P

V

A

E

E

L

R

A

G

S

-

Q

-

L

V

S

G

I

M

V

V

L

F

T

Q

E

S

Q

Y

I

A

A

L

S

Y

K

P

N

H

L

L

T

S

V

V

Q

A

E

E

L

N

V

M

A

S

L

F

G

G

R

-

Q

-

P

-

Y

-

T

L

N

K

W

L

V

A

G

G

K

A

M

K

A

K

E

E

E

V

D

I

I

N

E

Q

Q

R

I

V

R

N

K

Q

A

V

I

A

E

E

V

V

T

L

H

Q

I

L

G

A

D

H

L

L

V

L

A

D

K

R

R

K

C

P

F

K

E

A

L

L

S

S

D

G

G

G

Q

Q

L

R

Q

Q

K

R

V

V

L

A

I

I

A

G

R

R

A

T

I

L

A

V

Q

A

D

E

T

P

P

S

F

V

I

F

M

L

L

L

D

D

E

E

P

P

T

L

T

S

H

N

L

L

D

D

V

A

Y

A

H

L

K

R

A

V

Y

Q

I

M

L

R

K

I

L

E

L

I

K

S

R

R

L

L

A

H

F

K

E

R

T

L

R

G

K

R

T

T

I

M

L

I

F

Y

S

V

T

T

H

H

E

D

I

Q

D

V

F

E

A

A

I

M

Q

T

L

L

C

A

D

D

K

K

M

I

I

V

V

V

M

L

N

D

E

A

D

G

G

R

F

V

-

A

E

Q

F

V

G

G

R

K

P

P

K

L

E

E

L

L

binding adenosine-5'-diphosphate: V17 (≠ I28), S37 (≠ N48), G38 (= G49), G40 (= G51), K41 (= K52), S42 (= S53), T43 (= T54)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12

1f3oA Crystal structure of mj0796 atp-binding cassette (see paper)
30% identity, 78% coverage: 9:213/264 of query aligns to 1:209/232 of 1f3oA

query
sites
1f3oA

V

M

L

I

K

K

T

L

E

K

D

N

L

V

S

T

I

K

G

T

Y
|
Y

R

K

T

M

K

G

K

E

E

E

L

I

N

I

V

Y

I

A

A

L

A

K

D

N

I

V

N

N

L

L

K

N

L

I

H

K

E

E

G

G

E

E

L

F

V

V

G

S

L

I

V

M

G

G

A

P

N

S

G
|
G

I

S

G
|
G

K
|
K

S
|
S

T
|
T

L

M

L

L

R

N

T

I

L

I

S

G

R

C

V

L

Q

D

P

K

A

P

L

T

H

E

G

G

K

E

I

V

M

Y

L

I

S

D

D

N

I

I

E

K

L

T

Q

N

S

D

Y

L

K

D

S

D

V

D

E

E

L

L

A

T

-

K

-

I

-

R

S

D

Q

K

L

I

S

G

I

F

V

V

L

F

T

Q

E

Q

Q

F

I

N

A

L

S

I

K

P

N

L

L

L

T

T

V

A

Q

L

E

E

L

N

V

V

A

E

L

L

G

-

R

-

Q

-

P

P

Y

L

T

I

-

F

N

K

W

Y

V

R

G

G

K

A

M

M

A

S

E

G

E

E

D

-

I

-

E

E

Q

R

I

R

R

K

K

R

A

A

I

L

E

E

V

C

T

L

H

K

I

M

G

A

D

E

L

L

V

E

A

E

K

R

-

F

-

A

-

N

-

H

R

K

C

P

F

N

E

Q

L

L

S

S

D

G

G

G

Q

Q

L

Q

Q

Q

K

R

V

V

L

A

I

I

A

A

R

R

A

A

I

L

A

A

Q

N

D

N

T

P

P

P

F

I

I

I

M

L

L

A

D

D

E

E

P

P

T

T

T

G

H

A

L

L

D

D

V

S

Y

K

H

T

K

G

A

E

Y

K

I

I

L

M

K

Q

L

L

K

K

R

K

L

L

A

N

F

E

E

E

T

D

R

G

K

K

T

T

I

V

L

V

F

V

S

V

T

T

H

H

E

D

I

I

D

N

F

V

A

A

binding adenosine-5'-diphosphate: Y11 (= Y19), G41 (= G49), G43 (= G51), K44 (= K52), S45 (= S53), T46 (= T54)

P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
28% identity, 80% coverage: 27:236/264 of query aligns to 17:221/369 of P19566

query
sites
P19566

V

V

I

V

A

S

A

K

D

D

I

I

N

N

L

L

K

D

L

I

H

H

E

D

G

G

E

E

L

F

V

V

G

V

L

F

V

V

G

G

A

P

N

S

G

G

I

C

G

G

K

K

S

S

T

T

L

L

R

R

T

M

L

I

S

A

R

G

V

L

Q

E

P

T

A

I

L

T

H

S

G

G

K

D

I

L

M

F

L

I

S

G

D

E

I

T

E

R

L

M

Q

N

S

D

Y

I

K

P

S

P

V

A

E

E

L

R

A

G

S

-

Q

-

L

V

S

G

I

M

V

V

L

F

T

Q

E

S

Q

Y

I

A

A
x
L

S

Y

K

P

N

H

L

L

T

S

V

V

Q

A

E

E

L

N

V

M

A

S

L

F

G

G

R

-

Q

-

P

-

Y

-

T

L

N

K

W

L

V

A

G

G

K

A

M

K

A

K

E

E

E

V

D

M

I

N

E

Q

Q

R

I

V

R

N

K

Q

A

V

I

A

E

E

V

V

T

L

H

Q

I

L

G

A

D

H

L

L

V

L

A

E

K

R

R

K

C

P

F

K

E

A

L

L

S

S

D

G

G

G

Q

Q

L

R

Q

Q

K

R

V

V

L

A

I

I

A

G

R

R

A

T

I

L

A

V

Q

A

D

E

T

P

P

R

F

V

I

F

M

L

L

L

D

D

E

E

P
|
P

T

L

T

S

H

N

L

L

D
|
D

V

A

Y

A

H

L

K

R

A

V

Y

Q

I

M

L

R

K

I

L

E

L

I

K

S

R

R

L

L

A

H

F

K

E

R

T

L

R

G

K

R

T

T

I

M

L

I

F

Y

S

V

T

T

H

H

E

D

I

Q

D

V

F

E

A

A

I

M

Q

T

L

L

C

A

D

D

K

K

M

I

I

V

V

V

M

L

N

D

E

A

D

G

G

R

F

V

-

A

E

Q

F

V

G

G

R

K

P

P

K

L

E

E

L

L

L86 (≠ A98) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
P160 (= P176) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
D165 (= D181) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.

Sites not aligning to the query:

306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.

1l2tA Dimeric structure of mj0796, a bacterial abc transporter cassette (see paper)
30% identity, 78% coverage: 9:213/264 of query aligns to 1:209/230 of 1l2tA

query
sites
1l2tA

V

M

L

I

K

K

T

L

E

K

D

N

L

V

S

T

I

K

G

T

Y
|
Y

R

K

T

M

K

G

K

E

E

E

L

I

N

I

V

Y

I

A

A

L

A

K

D

N

I

V

N

N

L

L

K

N

L

I

H

K

E

E

G

G

E

E

L

F

V

V

G

S

L

I

V

M

G

G

A

P

N
x
S

G
|
G

I
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

M

L

L

R

N

T

I

L

I

S

G

R

C

V

L

Q

D

P

K

A

P

L

T

H

E

G

G

K

E

I

V

M

Y

L

I

S

D

D

N

I

I

E

K

L

T

Q

N

S

D

Y

L

K

D

S

D

V

D

E

E

L

L

A

T

-

K

-

I

-

R

S

D

Q

K

L

I

S

G

I

F

V

V

L

F

T

Q

E

Q

Q

F

I

N

A

L

S

I

K

P

N

L

L

L

T

T

V

A

Q

L

E

E

L

N

V

V

A

E

L

L

G

-

R

-

Q

-

P

P

Y

L

T

I

-

F

N

K

W

Y

V

R

G

G

K

A

M

M

A

S

E

G

E

E

D

-

I

-

E

E

Q

R

I

R

R

K

K

R

A

A

I

L

E

E

V

C

T

L

H

K

I

M

G

A

D

E

L

L

V

E

A

E

K

R

-
x
F

-

A

-

N

-

H

R

K

C

P

F

N

E
x
Q

L

L

S
|
S

D

G

G
|
G

Q
|
Q

L

Q

Q

Q

K

R

V

V

L

A

I

I

A

A

R

R

A

A

I

L

A

A

Q

N

D

N

T

P

P

P

F

I

I

I

M

L

L

A

D

D

E

Q

P

P

T

T

T

G

H

A

L

L

D

D

V

S

Y

K

H

T

K

G

A

E

Y

K

I

I

L

M

K

Q

L

L

K

K

R

K

L

L

A

N

F

E

E

E

T

D

R

G

K

K

T

T

I

V

L

V

F

V

S

V

T

T

H
|
H

E

D

I

I

D

N

F

V

A

A

binding adenosine-5'-triphosphate: Y11 (= Y19), S40 (≠ N48), G41 (= G49), S42 (≠ I50), G43 (= G51), K44 (= K52), S45 (= S53), T46 (= T54), F138 (vs. gap), Q145 (≠ E149), S147 (= S151), G149 (= G153), Q150 (= Q154), H204 (= H208)

3c4jA Abc protein artp in complex with atp-gamma-s
31% identity, 78% coverage: 32:236/264 of query aligns to 22:225/242 of 3c4jA

query
sites
3c4jA

I

I

N

N

L

V

K

H

L

I

H

R

E

E

G

G

E

E

L

V

V

V

G

V

L

V

V

I

G

G

A

P

N
x
S

G
|
G

I
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

R

R

T

C

L

L

S

N

R

L

V

L

Q

E

P

D

A

F

L

D

H

E

G

G

K

E

I

I

M

I

L

I

S

D

D

G

I

I

E

N

L

L

Q

K

S

A

Y

K

K

D

S

T

V

N

-

L

-

N

E

K

L

V

A

R

S

E

Q

E

L

V

S

G

I

M

V

V

L

F

T

Q

E

R

Q

F

I

N

A

L

S

F

K

P

N

H

L

M

T

T

V

V

Q

L

E

N

L

N

V

I

A

T

L

L

G

A

R

P

Q

M

P

K

Y

V

T

R

N

K

W

W

V

P

G

R

K

E

M

K

A

A

E

E

E

A

D

-

I

-

E

-

Q

-

I

-

R

-

K

K

A

A

I

M

E

E

V

L

-

L

-

D

-

K

T

V

H

G

I

L

G

K

D

D

L

K

V

A

A

H

K

A

R

Y

C

P

F

D

E

S

L

L

S

S

D

G

G

G

Q

Q

L

A

Q

Q

K

R

V

V

L

A

I

I

A

A

R

R

A

A

I

L

A

A

Q

M

D

E

T

P

P

K

F

I

I

M

M

L

L

F

D

D

E

E

P

P

T

T

T

S

H

A

L

L

D

D

V

P

Y

E

H

M

K

V

A

G

Y

E

I

V

L

L

K

S

L

V

L

M

K

K

R

Q

L

L

A

A

F

N

E

E

T

G

R

M

K

-

T

T

I

M

L

V

F

V

S

V

T

T

H

H

E

E

I

M

D

G

F

F

A

A

I

R

Q

E

L

V

C

G

D

D

K

R

M

V

I

L

V

F

M

M

N

D

E

-

D

G

G

G

F

Y

-

I

E

E

F

E

G

G

R

K

P

P

K

E

E

D

L

L

binding phosphothiophosphoric acid-adenylate ester: S38 (≠ N48), G39 (= G49), S40 (≠ I50), G41 (= G51), K42 (= K52), S43 (= S53), T44 (= T54)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 13, 18

3c41J Abc protein artp in complex with amp-pnp/mg2+
31% identity, 78% coverage: 32:236/264 of query aligns to 22:225/242 of 3c41J

query
sites
3c41J

I

I

N

N

L

V

K

H

L

I

H

R

E

E

G

G

E

E

L

V

V

V

G

V

L

V

V

I

G

G

A

P

N
x
S

G
|
G

I

S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

R

R

T

C

L

L

S

N

R

L

V

L

Q

E

P

D

A

F

L

D

H

E

G

G

K

E

I

I

M

I

L

I

S

D

D

G

I

I

E

N

L

L

Q

K

S

A

Y

K

K

D

S

T

V

N

-

L

-

N

E

K

L

V

A

R

S

E

Q

E

L

V

S

G

I

M

V

V

L

F

T

Q

E

R

Q

F

I

N

A

L

S

F

K

P

N

H

L

M

T

T

V

V

Q

L

E

N

L

N

V

I

A

T

L

L

G

A

R

P

Q

M

P

K

Y

V

T

R

N

K

W

W

V

P

G

R

K

E

M

K

A

A

E

E

E

A

D

-

I

-

E

-

Q

-

I

-

R

-

K

K

A

A

I

M

E

E

V

L

-

L

-

D

-

K

T

V

H

G

I

L

G

K

D

D

L

K

V

A

A

H

K

A

R

Y

C

P

F

D

E

S

L

L

S

S

D

G

G

G

Q

Q

L

A

Q

Q

K

R

V

V

L

A

I

I

A

A

R

R

A

A

I

L

A

A

Q

M

D

E

T

P

P

K

F

I

I

M

M

L

L

F

D
|
D

E

E

P

P

T

T

T

S

H

A

L

L

D

D

V

P

Y

E

H

M

K

V

A

G

Y

E

I

V

L

L

K

S

L

V

L

M

K

K

R

Q

L

L

A

A

F

N

E

E

T

G

R

M

K

-

T

T

I

M

L

V

F

V

S

V

T

T

H

H

E

E

I

M

D

G

F

F

A

A

I

R

Q

E

L

V

C

G

D

D

K

R

M

V

I

L

V

F

M

M

N

D

E

-

D

G

G

G

F

Y

-

I

E

E

F

E

G

G

R

K

P

P

K

E

E

D

L

L

binding phosphoaminophosphonic acid-adenylate ester: S38 (≠ N48), G39 (= G49), G41 (= G51), K42 (= K52), S43 (= S53), T44 (= T54)
binding magnesium ion: S43 (= S53), D163 (= D174)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 13, 18

2olkA Abc protein artp in complex with adp-beta-s
31% identity, 78% coverage: 32:236/264 of query aligns to 22:225/242 of 2olkA

query
sites
2olkA

I

I

N

N

L

V

K

H

L

I

H

R

E

E

G

G

E

E

L

V

V

V

G

V

L

V

V

I

G

G

A

P

N
x
S

G
|
G

I
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

R

R

T

C

L

L

S

N

R

L

V

L

Q

E

P

D

A

F

L

D

H

E

G

G

K

E

I

I

M

I

L

I

S

D

D

G

I

I

E

N

L

L

Q

K

S

A

Y

K

K

D

S

T

V

N

-

L

-

N

E

K

L

V

A

R

S

E

Q

E

L

V

S

G

I

M

V

V

L

F

T

Q

E

R

Q

F

I

N

A

L

S

F

K

P

N

H

L

M

T

T

V

V

Q

L

E

N

L

N

V

I

A

T

L

L

G

A

R

P

Q

M

P

K

Y

V

T

R

N

K

W

W

V

P

G

R

K

E

M

K

A

A

E

E

E

A

D

-

I

-

E

-

Q

-

I

-

R

-

K

K

A

A

I

M

E

E

V

L

-

L

-

D

-

K

T

V

H

G

I

L

G

K

D

D

L

K

V

A

A

H

K

A

R

Y

C

P

F

D

E

S

L

L

S

S

D

G

G

G

Q

Q

L

A

Q

Q

K

R

V

V

L

A

I

I

A

A

R

R

A

A

I

L

A

A

Q

M

D

E

T

P

P

K

F

I

I

M

M

L

L

F

D

D

E

E

P

P

T

T

T

S

H

A

L

L

D

D

V

P

Y

E

H

M

K

V

A

G

Y

E

I

V

L

L

K

S

L

V

L

M

K

K

R

Q

L

L

A

A

F

N

E

E

T

G

R

M

K

-

T

T

I

M

L

V

F

V

S

V

T

T

H

H

E

E

I

M

D

G

F

F

A

A

I

R

Q

E

L

V

C

G

D

D

K

R

M

V

I

L

V

F

M

M

N

D

E

-

D

G

G

G

F

Y

-

I

E

E

F

E

G

G

R

K

P

P

K

E

E

D

L

L

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: S38 (≠ N48), G39 (= G49), S40 (≠ I50), G41 (= G51), K42 (= K52), S43 (= S53), T44 (= T54)

Sites not aligning to the query:

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: 13, 18

2oljA Abc protein artp in complex with adp/mg2+
31% identity, 78% coverage: 32:236/264 of query aligns to 22:225/242 of 2oljA

query
sites
2oljA

I

I

N

N

L

V

K

H

L

I

H

R

E

E

G

G

E

E

L

V

V

V

G

V

L

V

V

I

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G

A

P

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x
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x
S

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G

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K

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S

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T

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Y

K

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-

L

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L

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L

binding adenosine-5'-diphosphate: S38 (≠ N48), G39 (= G49), S40 (≠ I50), G41 (= G51), K42 (= K52), S43 (= S53), T44 (= T54)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 13, 18

Q99758 Phospholipid-transporting ATPase ABCA3; ABC-C transporter; ATP-binding cassette sub-family A member 3; ATP-binding cassette transporter 3; ATP-binding cassette 3; Xenobiotic-transporting ATPase ABCA3; EC 7.6.2.1; EC 7.6.2.2 from Homo sapiens (Human) (see 15 papers)
29% identity, 81% coverage: 10:223/264 of query aligns to 530:736/1704 of Q99758

query
sites
Q99758

L

I

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N

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N568 (= N48) to D: in SMDP3; does not affect location in intracellular vesicle membrane; does not affect proteolytic cleavage; does not affect N-glycosylation; loss of ATP hydrolysis activity; decreases ATP binding in vitro; does not affect protein expression; does not affect multivesicular bodies and lamellar bodies location; affects multivesicular bodies and lamellar bodies development; loss of phosphatidylcholine transport; does not affect cholesterol transport; dbSNP:rs121909184
L579 (= L59) to P: in SMDP3; uncertain significance
R605 (≠ A86) to Q: in SMDP3; uncertain significance; dbSNP:rs760006956
S693 (≠ T178) mutation to L: Does not affect protein oligomerization.

Sites not aligning to the query:

43 R → L: in SMDP3; uncertain significance
53 N→Q: Does not affect N-glycosylation. Does not affect protein expression. Does not affect lamellar body membrane location.
101 L → P: in SMDP3; loss of intracellular vesicle membrane location; loss of proteolytic cleavage; does not affect N-glycosylation; loss of ATP hydrolysis activity; decreases ATP binding in vitro; dbSNP:rs121909182
124 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→Q: Loss of N-glycosylation. Reduces protein expression by 50%. Affects anterograde trafficking; when associated with Q-140. Reduces protein expression by 85%; when associated with Q-140. Does not affect lamellar body membrane location.
140 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N → H: in dbSNP:rs45447801; N→Q: Loss of N-glycosylation. Reduces protein expression by 50%. Affects anterograde trafficking; when associated with Q-124. Reduces protein expression by 85%; when associated with Q-140. Does not affect lamellar body membrane location.
173:174 LK→AA: Loss of proteolytic processing.
174:175 Cleavage; by CTSL
215 Q → K: in SMDP3; loss of lamellar bodies membrane location; loss of proteolytic cleavage; increases cellular free cholesterol and phosphatidylcholine transport; loss of vesicles formation; increases free cholesterol induced cell death; loss of protein oligomerization; dbSNP:rs879159551
280 R → C: in SMDP3; uncertain significance; does not affect protein oligomerization; dbSNP:rs201299260
288 R → K: in SMDP3; uncertain significance; does not affect protein oligomerization; dbSNP:rs117603931
290 L → M: in a breast cancer sample; somatic mutation
292 E → V: in SMDP3; uncertain significance; does not affect lamellar bodies membrane location; does not affect proteolytic cleavage; affects lamellar bodies formation; does not affect cholesterol and phosphatidylcholine transport; decreases vesicles formation; does not affect free cholesterol induced cell death; dbSNP:rs149989682
766 P → S: in dbSNP:rs45592239
801 E → D: in a breast cancer sample; somatic mutation
945 N→Q: Does not affect lamellar body membrane location. Does not affect protein expression. Does not affect proteolytic processing.
982 L → P: in SMDP3; loss of intracellular vesicle membrane location; loss of proteolytic cleavage; does not affect N-glycosylation; dbSNP:rs1402761450
1069 H → Q: in a breast cancer sample; somatic mutation
1076 N → K: in SMDP3; uncertain significance
1221 G → S: in SMDP3; does not affect intracellular vesicle membrane location; does not affect proteolytic cleavage; does not affect N-glycosylation; loss of ATP hydrolysis activity; G→A: Decreases ATP hydrolysis activity of 15% compared to the wild-type.; G→T: Decreases ATP hydrolysis activity of 36% compared to the wild-type.; G→V: Decreases ATP hydrolysis activity of 18% compared to the wild-type.
1302 G → E: in SMDP3; uncertain significance
1388 K → N: in SMDP3; decreases phosphatidylcholine transport; increases protein abundance; does not affect folding in the endoplasmic reticulum; decreases proteolytic processing; affects lamellar bodies development; reduces free cholesterol transport
1553 L → P: in SMDP3; loss of intracellular vesicle membrane location; loss of proteolytic cleavage; does not affect N-glycosylation; dbSNP:rs121909183
1580 L → P: in SMDP3; does not affect location in intracellular vesicle membrane; does not affect proteolytic cleavage; does not affect N-glycosylation; loss of ATP hydrolysis activity; decreases ATP binding in vitro; affects the intracellular vesicles development; decreases phosphatidylcholine transport; L→A: Decreases ATP hydrolysis activity of 13% compared to the wild-type.; L→F: Decreases ATP hydrolysis activity of 13% compared to the wild-type.; L→V: Decreases ATP hydrolysis activity of 56% compared to the wild-type.
1591 Q → P: in SMDP3; loss of intracellular vesicle membrane location; loss of proteolytic cleavage; does not affect N-glycosylation; dbSNP:rs28936691

7w02A Cryo-em structure of atp-bound abca3 (see paper)
29% identity, 81% coverage: 10:223/264 of query aligns to 495:701/1566 of 7w02A

query
sites
7w02A

L

I

K

K

T

I

E

K

D

H

L

L

S

S

I

K

G

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Y
x
F

R

R

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V

K

G

K

N

E

K

L

D

N

R

V

A

I

A

A

V

A

R

D

D

I

L

N

N

L

L

K

N

L

L

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Y

E

E

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G

E

Q

L

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T

G

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L

L

V

L

G

G

A

H

N
|
N

G
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G

I

A

G
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G

K
|
K

S
x
T

T
|
T

L

T

L

L

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T

M

L

L

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L

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P

A

P

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S

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A

M

Y

L

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S

D

G

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E

E

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S

S

Y

Q

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V

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L

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L

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I

L

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C

L

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T
x
Q

E

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D

I

I

A

L

S

F

K

D

N

N

L

L

T

T

V

V

Q

A

E

E

L

H

V

L

A

Y

L

F

G

-

R

-

Q

-

P

-

Y

Y

T

A

N

Q

W

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G

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D

L

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A

N

K

S

R

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C

S

F

R

E

F

L
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L

S
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S

D

G

G

G

Q

M

L

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Q

R

K

K

V

L

L

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I

A

G

R

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A

A

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A

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A

R

Y

A

I

I

L

W

K

D

L

L

K

Q

R

R

L

Q

A

-

F

-

E

K

T

S

R

D

K

R

T

T

I

I

L

V

F

L

S

T

T

T

H

H

E

F

I

M

D

D

F

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A

A

I

D

Q

L

L

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D

D

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A

V

I

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M

binding adenosine-5'-triphosphate: F504 (≠ Y19), N533 (= N48), G534 (= G49), G536 (= G51), K537 (= K52), T538 (≠ S53), T539 (= T54), Q578 (≠ T94), L630 (= L150), S631 (= S151)
binding magnesium ion: T538 (≠ S53), Q578 (≠ T94)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 1277, 1305, 1306, 1307, 1308, 1309, 1310, 1344, 1395, 1398
binding magnesium ion: 1310, 1344

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
30% identity, 83% coverage: 19:236/264 of query aligns to 8:223/240 of 4ymuJ

query
sites
4ymuJ

Y

Y

R

K

T

N

K
x
F

K

G

E

S

L

L

N

E

V
|
V

I

L

A

K

A

G

D

-

I

V

N

T

L

L

K

K

L

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H

N

E

K

G

G

E

E

L

V

V

V

G

V

L

I

V

I

G

G

A

P

N
x
S

G
|
G

I
x
S

G
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G

K
|
K

S
|
S

T
|
T

L

L

R

R

T

C

L

I

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N

R

L

V

L

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A

P

L

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H

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G

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E

I

V

M

F

L

I

S

D

D

G

I

V

E

K

L

I

Q

N

S

N

Y

G

K

K

S

V

-

N

-

I

V

N

E

K

L

V

A

R

S

Q

Q

K

L

V

S

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I

M

V

V

L

F

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H

Q

F

I

N

A

L

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N

H

L

L

T

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V

A

Q

I

E

E

L

N

V

I

A

T

L

L

G

A

R

-

Q

-

P

P

Y

V

T

K

N

-

W

-

V

V

G

K

K

K

M

M

A

N

E

K

D

E

I

A

E

E

Q

E

I

L

R

-

K

-

A

A

I

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V

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L

H

A

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G

V

D

G

L

L

V

L

A

D

K

K

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K

-

D

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-

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L

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Q

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V

L

L

A

I

I

A

A

R

R

A

A

I

L

A

A

Q

M

D

Q

T

P

P

E

F

V

I

M

M

L

L

F

D

D

E
|
E

P

P

T

T

T

S

H

A

L

L

D

D

V

P

Y

E

H

M

K

V

A

K

Y

E

I

V

L

L

K

N

L

V

L

M

K

K

R

Q

L

L

A

A

F

N

E

E

T

G

R

M

K

-

T

T

I

M

L

V

F

V

S

V

T

T

H
|
H

E

E

I

M

D

G

F

F

A

A

I

R

Q

E

L

V

C

G

D

D

K

R

M

V

I

I

V

F

M

M

N

-

E

D

D

D

G

G

-

V

-

I

F

V

E

E

F

E

G

G

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T

P

P

K

E

E

E

L

I

binding adenosine-5'-triphosphate: F11 (≠ K22), V16 (= V27), S36 (≠ N48), G37 (= G49), S38 (≠ I50), G39 (= G51), K40 (= K52), S41 (= S53), T42 (= T54), E162 (= E175), H194 (= H208)
binding magnesium ion: S41 (= S53), E162 (= E175)

Query Sequence

>WP_024771162.1 NCBI__GCF_000520995.1:WP_024771162.1
MNREALNIVLKTEDLSIGYRTKKELNVIAADINLKLHEGELVGLVGANGIGKSTLLRTLS
RVQPALHGKIMLSDIELQSYKSVELASQLSIVLTEQIASKNLTVQELVALGRQPYTNWVG
KMAEEDIEQIRKAIEVTHIGDLVAKRCFELSDGQLQKVLIARAIAQDTPFIMLDEPTTHL
DVYHKAYILKLLKRLAFETRKTILFSTHEIDFAIQLCDKMIVMNEDGFEFGRPKELVQCR
AFSSLFPEDLILFDSKSGRYKVRN

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory