SitesBLAST

O59010 Glutamate transporter homolog; Glt(Ph); Sodium-aspartate symporter Glt(Ph); Sodium-dependent aspartate transporter from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 3 papers)
34% identity, 91% coverage: 25:431/445 of query aligns to 15:418/425 of O59010

query
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O59010

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S65 (= S81) mutation to V: Strongly decreased chloride conductance.
R276 (≠ A294) mutation to S: Increased rate of aspartate transport; when associated with R-395.
RSS 276:278 (≠ ASS 294:296) binding L-aspartate
M311 (= M330) mutation to A: Decreased dependence of aspartate binding on Na(+) concentration.
T314 (= T333) binding L-aspartate
V355 (≠ I374) binding L-aspartate
D394 (≠ G407) binding L-aspartate
M395 (= M408) mutation to R: Increased rate of aspartate transport; when associated with S-276.
R397 (= R410) mutation to A: Strongly decreased affinity for aspartate.
N401 (= N414) binding L-aspartate
D405 (= D418) mutation to N: Strongly decreased affinity for aspartate.

6xwnB Structure of glutamate transporter homologue glttk in the presence of tboa inhibitor (see paper)
35% identity, 94% coverage: 10:427/445 of query aligns to 2:414/426 of 6xwnB

query
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6xwnB

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binding (3s)-3-(benzyloxy)-l-aspartic acid: S275 (= S295), S276 (= S296), M310 (= M330), T313 (= T333), D394 (≠ G407), R397 (= R410)

6zl4A The structure of glutamate transporter homologue glttk in complex with the photo switchable compound (cis) (see paper)
35% identity, 93% coverage: 13:427/445 of query aligns to 2:411/424 of 6zl4A

query
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6zl4A

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L

G

D

M

M

F

G

R
|
R

T
|
T

A

M

V

V

N

N

V

V

T

T

G

G

D

D

L

L

T

A

A

G

S

T

V

V

I

I

F

V

N

A

K

K

binding decyl-beta-d-maltopyranoside: L3 (= L14), L191 (≠ I214), G195 (≠ W218), R282 (≠ K305)
binding (2~{S},3~{S})-2-azanyl-3-[[4-[2-(4-methoxyphenyl)hydrazinyl]phenyl]methoxy]butanedioic acid: R271 (≠ A294), S272 (= S295), S273 (= S296), M307 (= M330), T310 (= T333), G353 (= G376), A354 (≠ G377), R394 (= R410), T395 (= T411)

6zgbA Glutamate transporter homologue glttk in complex with a photo cage compound (see paper)
35% identity, 93% coverage: 13:427/445 of query aligns to 3:412/425 of 6zgbA

query
sites
6zgbA

Y

Y

L

L

D

D

R

Y

L

P

I

V

K

-

G

-

R

-

L

L

W

W

L

-

K

K

V

I

I

L

I

W

G

G

L

L

I

V

L

L

G

G

A

A

G

V

V

F

G

G

I

L

V

I

I

A

N

G

P

H

S

F

T

-

G

-

F

-

V

-

P

-

E

-

N

G

F

Y

S

A

L

G

L

A

L

V

A

K

N

T

W

Y

L

I

D

K

L

P

P

F

G

G

Q

D

I

L

F

F

M

V

R

R

L

L

V

L

Q

K

M

M

I

L

M

V

I

M

P

P

L

I

I

V

F

L

A

A

S

S

I

L

I

V

S

V

G

G

I

A

V

A

G

S

N

I

T

S

S

P

E

A

N

R

L

L

K

G

A

R

F

V

G

G

I

V

K

K

L

I

L

V

Y

Y

F

Y

V

L

F

A

T

T

T

S

V

A

I

M

A

A

I

V

T

F

I

F

G

G

L

L

I

I

V

V

T

-

F

-

I

-

M

-

K

-

P

-

G

-

Q

G

Y

R

V

L

F

F

K

N

L

V

G

G

G

A

F

N

P

V

N

N

S

L

G

G

E

S

-

G

-

T

-

G

K

K

L

A

I

I

E

E

A

A

N

-

E

Q

Q

P

T

P

N

S

L

L

I

V

E

Q

N

-

I

-

P

-

D

-

T

T

I

L

S

L

K

N

L

I

I

V

P

P

N

T

N

N

P

P

L

F

E

A

S

S

I

L

L

A

M

K

G

G

E

E

M

V

L

L

G

P

V

V

V

I

I

F

F

F

T

A

I

I

I

L

G

G

V

I

A

A

I

I

T

T

Q

Y

L

L

-

M

-

N

-

R

N

N

H

E

D

E

-

R

-

V

-

R

-

K

T

S

A

A

R

E

P

T

I

L

R

R

F

V

V

F

E

D

A

G

I

L

Q

A

K

E

I

A

C

M

M

Y

I

L

V

I

I

V

S

G

W

G

A

V

M

M

M

Q

L

Y

V

A

P

P

Y

I

A

G

V

V

F

F

G

A

L

L

I

I

A

A

A

Y

L

V

L

M

S

A

K

E

I

Q

G

G

I

V

E

R

I

V

F

V

L

G

G

P

L

L

G

A

Y

K

Y

V

M

V

I

G

V

A

V

V

I

Y

L

T

G

G

L

L

I

F

L

L

L

Q

M

I

I

V

-

I

-

T

-

Y

F

F

Y

I

L

L

I

L

M

K

V

V

F

F

A

G

F

I

T

-

R

-

K

-

N

D

P

P

L

I

K

K

F

F

L

I

N

R

L

K

I

A

R

K

E

D

P

A

Q

M

L

I

L

T

A

A

F

F

S

V

T

T

A
x
R

S
|
S

A

S

A

G

V

T

M

L

P

P

V

V

S

T

M

M

K

R

T

V

A

A

D

E

E

E

K

E

L

M

G

G

V

V

S

D

S

K

N

G

I

I

S

F

D

S

F

F

V

T

I

L

P

P

V

L

G

G

A

A

T

T

I

I

N

N

M
|
M

D

D

G

G

T

T

A

A

L

L

F

Y

Q

Q

C

G

V

V

T

T

T

V

L

L

F

F

M

V

A

A

Q

N

A

A

Y

I

G

G

I

H

E

P

L

L

S

T

V

L

I

G

N

Q

L

Q

I

L

L

V

I

V

T

V

T

L

T

T

V

A

V

V

A

L

A

A

S

S

I

I

G

G

T

T

P

A

A

G

I

V

P

P

G

G

G

A

G

G

V

A

I

I

I

M

L

L

A

A

S

M

V

V

L

L

Q

Q

S

S

A

V

G

G

I

L

P

D

I

L

D

T

-

P

-

G

-

S

-

P

-

V

-

A

-

L

G

A

L

Y

I

A

I

M

I

I

I

L

G

G

I

I

D

D

R

A

I

I

L

L

G
x
D

M

M

F

G

R
|
R

T
|
T

A

M

V

V

N

N

V

V

T

T

G

G

D

D

L

L

T

A

A

G

S

T

V

V

I

I

F

V

N

A

K

K

binding (2~{S},3~{S})-2-azanyl-3-[(2-nitrophenyl)methoxy]butanedioic acid: R272 (≠ A294), S273 (= S295), S274 (= S296), M308 (= M330), D392 (≠ G407), R395 (= R410), T396 (= T411)

6x15A Inward-facing state of the glutamate transporter homologue gltph in complex with l-aspartate and sodium ions (see paper)
34% identity, 91% coverage: 25:431/445 of query aligns to 15:418/419 of 6x15A

query
sites
6x15A

K

K

V

I

I

L

I

I

G

G

L

L

I

I

L

L

G

G

A

A

G

I

V

V

G

G

I

L

V

I

I

L

N

G

P

H

S

-

T

-

G

-

F

-

V

-

P

-

E

Y

N

G

F

Y

S

A

L

H

L

A

L

V

A

H

N

T

W

Y

L

V

D

K

L

P

P
x
F

G

G

Q

D

I

L

F

F

M

V

R

R

L

L

V

L

Q

C

M

M

I

L

M

V

I

M

P

P

L

I

I

V

F

F

A

A

S

S

I

L

I

V

S

V

G

G

I

A

V

A

G

S

N

I

T

S

S
x
P

E

A

N

R

L

L

K

G

A

R

F

V

G

G

I

V

K

K

L

I

L

V

Y

Y

F
x
Y

V

L

F
x
L

T
|
T

T
x
S

V

A

I
x
F

A

A

I

V

T

T

I

L

G

G

L

I

I

I

V

M

T

A

F

R

I

L

M

F

K

N

P

P

G

G

Q

A

Y

-

V

-

F

-

K

-

L

-

G

G

G

I

F

H

P

L

N

A

S

V

G

G

E

G

K

Q

L

Q

I

F

E

Q

A

P

N

H

E

Q

Q

A

T

P

N

P

L
|
L

I

V

E

H

N
x
I

I

L

P

L

D

D

T

-

I

-

S

-

K

-

L

I

I

V

P

P

N

T

N

N

P

P

L

F

E

G

S

A

I

L

L

A

M

N

G

G

E

Q

M

V

L

L

G

P

V

T

V

I

I

F

F

F

T

A

I
|
I

I

I

I

L

G

G

V

I

A

A

I

I

T

T

Q
x
Y

L

L

N

M

H

N

D

S

-

E

-

N

-

E

-

K

-

V

-

R

-

K

T

S

A

A

R

E

P

T

I

L

R

D

F

A

V

I

E

N

A

G

I

L

Q

A

K

E

I

A

C

M

M

Y

I
x
K

V

I

I

V

S

N

W
x
G

A

V

M

M

M

Q

L

Y

V

A

P

P

Y
x
I

A

G

V

V

F
|
F

G

A

L

L

I

I

A

A

A

Y

L

V

L

M

S

A

K

E

I

Q

G

G

I

V

E

H

I

V

F

V

L

G

G

E

L

L

G

A

Y

K

Y

V

M

T

I

A

V

A

V

V

I

Y

L

V

G

G

L

L

-

T

-

L

-

Q

I

I

L

L

M

V

I

Y

F

F

Y

V

L
|
L

I

L

M

K

V

I

F

Y

A

G

F

I

T

-

R

-

K

-

N

D

P

P

L

I

K

S

F

F

L
x
I

N

K

L

H

I

A

R

K

E

D

P

A

Q
x
M

L

L

L

T

A

A

F

F

S

V

T

T

A

R

S
|
S

A

S

A

G

V

T

M

L

P

P

V

V

S

T

M

M

K

R

T

V

A

A

D

K

E

E

K

-

L

M

G

G

V

I

S

S

S

E

N

G

I

I

S

Y

D

S

F

F

V

T

I

L

P

P

V

L

G
|
G

A

A

T
|
T

I

I

N
|
N

M
|
M

D
|
D

G

G

T
|
T

A

A

L

L

F

Y

Q

Q

C

G

V

V

T

A

T

T

L

F

F

F

M

I

A

A

Q

N

A

A

Y

L

G

G

I

S

E

H

L

L

S
x
T

V
|
V

I
x
G

N

Q

L

Q

I

L

L
x
T

I

I

T

V

T
x
L

T

T

V

A

V

V

A

L

A

A

S
|
S

I
|
I

G

G

T
|
T

P

A

A
x
G

I

V

P

P

G

G

G
x
A

G
|
G

V

A

I

I

I

M

L

L

A

C

S

M

V

V

L

L

Q

H

S

S

A

V

G

G

I

L

P

P

I

L

D

T

-

D

-

P

-

N

-

V

-

A

G

A

L

Y

I

A

I

M

I

I

I

L

G

G

I

I

D

D

R

A

I

I

L

L

G
x
D

M

M

F

G

R
|
R

T
|
T

A
x
M

V

V

N
|
N

V
|
V

T

T

G

G

D
|
D

L

L

T

T

A

G

S

T

V

A

I

I

F

V

N

A

K

K

F

T

Y

E

G

G

S

T

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: F46 (≠ P62), F46 (≠ P62), P75 (≠ S91), L91 (≠ F107), F95 (≠ I111), L130 (= L151), I133 (≠ N154), I159 (= I184), Y167 (≠ Q192), K196 (≠ I214), G200 (≠ W218), I207 (≠ Y225), F210 (= F228), L250 (= L265), I262 (≠ L280), M269 (≠ Q287), T334 (≠ S353), V335 (= V354), G336 (≠ I355), T340 (≠ L359), L343 (≠ T362), M399 (≠ A412)
binding aspartic acid: S277 (= S295), S278 (= S296), T314 (= T333), G354 (≠ A373), A358 (≠ G377), G359 (= G378), D394 (≠ G407), R397 (= R410), T398 (= T411)
binding sodium ion: Y89 (≠ F105), T92 (= T108), S93 (≠ T109), G306 (= G325), T308 (= T327), N310 (= N329), N310 (= N329), M311 (= M330), D312 (= D331), S349 (= S368), I350 (= I369), T352 (= T371), N401 (= N414), V402 (= V415), D405 (= D418)

Sites not aligning to the query:

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: 4, 7

6x14A Inward-facing state of the glutamate transporter homologue gltph in complex with tfb-tboa (see paper)
34% identity, 91% coverage: 25:427/445 of query aligns to 12:411/413 of 6x14A

query
sites
6x14A

K

K

V
x
I

I

L

I

I

G

G

L

L

I

I

L

L

G

G

A

A

G

I

V

V

G

G

I

L

V

I

I

L

N

G

P

H

S

-

T

-

G

-

F

-

V

-

P

-

E

Y

N

G

F

Y

S

A

L

H

L

A

L

V

A

H

N

T

W

Y

L

V

D

K

L

P

P

F

G

G

Q

D

I

L

F

F

M

V

R

R

L

L

V

L

Q

C

M

M

I

L

M

V

I

M

P

P

L

I

I

V

F

F

A

A

S

S

I

L

I

V

S

V

G
|
G

I

A

V

A

G

S

N

I

T

S

S

P

E

A

N

R

L

L

K

G

A

R

F

V

G

G

I

V

K

K

L

I

L
x
V

L

V

Y

Y

F

Y

V

L

F

L

T

T

T

S

V

A

I

F

A

A

I

V

T

T

I

L

G

G

L

I

I

I

V

M

T

A

F

R

I

L

M

F

K

N

P

P

G

G

Q

A

Y

-

V

-

F

-

K

-

L

-

G

G

G

I

F

H

P

L

N

A

S

V

G

G

E

G

K

Q

L

Q

I

F

E

Q

A

P

N

H

E

Q

Q

A

T

P

N

P

L

L

I

V

E

H

N

I

I

L

P

L

D

D

T

-

I

-

S

-

K

-

L

I

I

V

P

P

N

T

N

N

P

P

L

F

E

G

S

A

I

L

L

A

M

N

G

G

E

Q

M

V

L

L

G

P

V

T

V

I

I

F

F

F

T

A

I

I

I
|
I

I

L

G

G

V

I

A

A

I

I

T

T

Q
x
Y

L

L

N

M

H

N

D

S

-

E

-

N

-

E

-

K

-

V

-

R

-

K

T

S

A

A

R

E

P

T

I

L

R

D

F

A

V

I

E

N

A

G

I

L

Q

A

K

E

I

A

C

M

M

Y

I
x
K

V

I

I

V

S

N

W

G

A

V

M
|
M

M

Q

L

Y

V

A

P

P

Y

I

A

G

V

V

F

F

G

A

L

L

I

I

A

A

A

Y

L

V

L

M

S

A

K

E

I

Q

G

G

I

V

E

H

I

V

F

V

L

G

G

E

L

L

G

A

Y

K

Y

V

M

T

I

A

V

A

V

V

I

Y

L

V

G

G

L

L

-

T

-

L

-

Q

I

I

L

L

M

V

I

Y

F

F

Y

V

L

L

I

L

M

K

V

I

F

Y

A

G

F

I

T

-

R

-

K

-

N

D

P

P

L

I

K

S

F

F

L

I

N

K

L

H

I

A

R

K

E

D

P

A

Q

M

L

L

L

T

A

A

F

F

S

V

T

T

A

R

S

S

S
|
S

A

S

A

G

V

T

M

L

P

P

V

V

S

T

M

M

K

R

T

V

A

A

D

K

E

E

K

-

L

M

G

G

V

I

S

S

S

E

N

G

I

I

S

Y

D

S

F

F

V

T

I

L

P

P

V

L

G

G

A

A

T
|
T

I
|
I

N

N

M

M

D

D

G

G

T
|
T

A

A

L

L

F

Y

Q

Q

C

G

V

V

T

A

T

T

L

F

F

F

M

I

A

A

Q

N

A

A

Y

L

G

G

I

S

E

H

L

L

S

T

V

V

I

G

N

Q

L

Q

I

L

L

T

I

I

T

V

T

L

T

T

V

A

V

V

A

L

A

A

S

S

I
|
I

G

G

T

T

P

A

A

G

I

V

P

P

G

G

G

A

G
|
G

V

A

I

I

I

M

L

L

A

C

S

M

V

V

L

L

Q

H

S

S

A

V

G

G

I

L

P

P

I

L

D

T

-

D

-

P

-

N

-

V

-

A

G

A

L

Y

I

A

I

M

I

I

I
x
L

G

G

I

I

D

D

R

A

I

I

L

L

G
x
D

M

M

F

G

R
|
R

T

T

A

M

V

V

N

N

V

V

T

T

G

G

D

D

L

L

T

T

A

G

S

T

V

A

I

I

F

V

N

A

K

K

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: G66 (= G85), V83 (≠ L102), I157 (= I185), Y164 (≠ Q192), K193 (≠ I214), T305 (= T327), I306 (= I328), I347 (= I369)
binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I13 (≠ V26), M199 (= M220), S275 (= S296), T311 (= T333), G356 (= G378), L384 (≠ I400), D391 (≠ G407), R394 (= R410)

Sites not aligning to the query:

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: 4

6bavA Crystal structure of gltph r397c in complex with s-benzyl-l-cysteine (see paper)
34% identity, 91% coverage: 25:430/445 of query aligns to 7:409/409 of 6bavA

query
sites
6bavA

K

K

V

I

I

L

I

I

G

G

L

L

I

I

L

L

G

G

A

A

G

I

V

V

G

G

I

L

V

I

I

L

N

G

P

H

S

-

T

-

G

-

F

-

V

-

P

-

E

Y

N

G

F

Y

S

A

L

D

L

A

L

V

A

K

N

T

W

Y

L

V

D

K

L

P

P

F

G

G

Q

D

I

L

F

F

M

V

R

R

L

L

V

L

Q

K

M

M

I

L

M

V

I

M

P

P

L

I

I

V

F

F

A

A

S

S

I

L

I

V

S

V

G

G

I

A

V

A

G

S

N

I

T

S

S

P

E

A

N

R

L

L

K

G

A

R

F

V

G

G

I

V

K

K

L

I

L

V

Y

Y

F

Y

V

L

F

L

T

T

T

S

V

A

I

F

A

A

I

V

T

T

I

L

G

G

L

I

I

I

V

M

T

A

F

R

I

L

M

F

K

N

P

P

G

G

Q

A

Y

G

V

I

-

H

F

L

K

A

L

V

G

G

F

-

P

-

N

-

S

-

G

-

E

-

K

Q

L

Q

I

F

E

Q

A

P

N

K

E

Q

Q

A

T

P

N

P

L

L

I

V

E

K

N

I

I

L

P

L

D

D

T

-

I

-

S

-

K

-

L

I

I

V

P

P

N

T

N

N

P

P

L

F

E

G

S

A

I

L

L

A

M

N

G

G

E

Q

M

V

L

L

G

P

V

T

V

I

I

F

F

F

T

A

I

I

I

L

G

G

V

I

A

A

I

I

T

T

Q

Y

L

L

N

M

H

N

D

S

-

E

-

N

-

E

-

K

-

V

-

R

-

K

T

S

A

A

R

E

P

T

I

L

R

D

F

A

V

I

E

N

A

G

I

L

Q

A

K

E

I

A

C

M

M

Y

I

K

V

I

I

V

S

N

W

G

A

V

M

M

M

Q

L

Y

V

A

P

P

Y

I

A

G

V

V

F

F

G

A

L

L

I

I

A

A

A

Y

L

V

L

M

S

A

K

E

I

Q

G

G

I

V

E

K

I

V

F

V

L

G

G

E

L

L

G

A

Y

K

Y

V

M

T

I

A

V

A

V

V

I

Y

L

V

G

G

L

L

-

T

-

L

-

Q

I

I

L

L

M

V

I

Y

F

F

Y

V

L

L

I

L

M

K

V

I

F

Y

A

G

F

I

T

-

R

-

K

-

N

D

P

P

L

I

K

S

F

F

L

I

N

K

L

K

I

A

R

K

E

D

P

A

Q

M

L

L

L

T

A

A

F

F

S

V

T

T

A
x
R

S

S

S
|
S

A

S

A

G

V

T

M

L

P

P

V

V

S

T

M

M

K

R

T

V

A

A

D

K

E

E

K

-

L

M

G

G

V

I

S

S

S

E

N

G

I

I

S

Y

D

S

F

F

V

T

I

L

P

P

V

L

G

G

A

A

T

T

I

I

N

N

M

M

D

D

G

G

T
|
T

A

A

L

L

F
x
Y

Q

Q

C

G

V

V

T

C

T

T

L

F

F

F

M

I

A

A

Q

N

A

A

Y

L

G

G

I

S

E

H

L

L

S

T

V

V

I

G

N

Q

L

Q

I

L

L

T

I

I

T

V

T

L

T

T

V

A

V

V

A

L

A

A

S

S

I

I

G

G

T

T

P

A

A

G

I

V

P

P

G

G

G

A

G

G

V

A

I

I

I

M

L

L

A

A

S

M

V

V

L

L

Q

E

S

S

A

V

G

G

I

L

P

P

I

L

D

T

-

D

-

P

-

N

-

V

-

A

G

A

L

Y

I

A

I

M

I

I

I

L

G

G

I

I

D
|
D

R

A

I

I

L

L

G
x
D

M

M

F

G

R
x
C

T
|
T

A

M

V

V

N

N

V

V

T

T

G

G

D

D

L

L

T

T

A

G

S

T

V

A

I

I

F

V

N

A

K

K

F

T

Y

E

G

G

binding benzylcysteine: R268 (≠ A294), S270 (= S296), T306 (= T333), Y309 (≠ F336), D382 (= D403), D386 (≠ G407), C389 (≠ R410), T390 (= T411)

6r7rA Crystal structure of the glutamate transporter homologue glttk in complex with d-aspartate (see paper)
34% identity, 91% coverage: 22:427/445 of query aligns to 4:403/416 of 6r7rA

query
sites
6r7rA

L

L

W

W

L

-

K

K

V

I

I

L

I

W

G

G

L

L

I

V

L

L

G

G

A

A

G

V

V

F

G

G

I

L

V

I

I

A

N

G

P
x
H

S

F

T

-

G

-

F

-

V

-

P

-

E

-

N

G

F

Y

S

A

L

G

L

A

L

V

A

K

N

T

W

Y

L

I

D

K

L

P

P

F

G

G

Q

D

I

L

F

F

M

V

R

R

L

L

V

L

Q

K

M

M

I

L

M

V

I

M

P

P

L

I

I

V

F

L

A

A

S

S

I

L

I

V

S

V

G

G

I

A

V

A

G

S

N

I

T

S

S

P

E

A

N

R

L

L

K

G

A

R

F

V

G

G

I

V

K

K

L

I

L

V

Y

Y

F

Y

V

L

F

A

T

T

T

S

V

A

I

M

A

A

I

V

T

F

I

F

G

G

L

L

I

I

V

V

T

-

F

-

I

-

M

-

K

-

P

-

G

-

Q

G

Y

R

V

L

F

F

K

N

L

V

G

G

G

A

F

N

P

V

N

N

S

L

G

G

E

S

K

G

L

-

I

-

E

T

A

G

N

A

E

Q

Q

P

T

P

N

S

L

L

I

V

E

Q

N

-

I

-

P

-

D

-

T

T

I

L

S

L

K

N

L

I

I

V

P

P

N

T

N

N

P

P

L

F

E

A

S

S

I

L

L

A

M

K

G

G

E

E

M

V

L

L

G

P

V

V

V

I

I

F

F

F

T

A

I

I

I

L

G

G

V

I

A

A

I

I

T

T

Q

Y

L

L

-

M

-

N

-

R

N

N

H

E

D

E

-

R

-

V

-

R

-

K

T

S

A

A

R

E

P

T

I

L

R

R

F

V

V

F

E

D

A

G

I

L

Q

A

K

E

I

A

C

M

M

Y

I

L

V

I

I

V

S

G

W

G

A

V

M

M

M

Q

L

Y

V

A

P

P

Y

I

A

G

V

V

F

F

G

A

L

L

I

I

A

A

A

Y

L

V

L

M

S

A

K

E

I

Q

G

G

I

V

E

R

I

V

F
x
V

L

G

G

P

L

L

G
x
A

Y

K

Y

V

M

V

I

G

V

A

V

V

I

Y

L

T

G

G

L

L

I

F

L

L

L

Q

M

I

I

V

-

I

-

T

-

Y

F

F

Y

I

L

L

I

L

M

K

V

V

F

F

A

G

F

I

T

-

R

-

K

-

N

D

P

P

L

I

K

K

F

F

L

I

N

R

L

K

I

A

R

K

E

D

P

A

Q

M

L

I

L

T

A

A

F

F

S

V

T

T

A
x
R

S

S

S
|
S

A

S

A

G

V

T

M

L

P

P

V

V

S

T

M

M

K

R

T

V

A

A

D

E

E

E

K

E

L

M

G

G

V

V

S

D

S

K

N

G

I

I

S

F

D

S

F

F

V

T

I

L

P

P

V

L

G

G

A

A

T

T

I

I

N

N

M
|
M

D

D

G

G

T
|
T

A

A

L

L

F

Y

Q

Q

C

G

V

V

T

T

T

V

L

L

F

F

M

V

A

A

Q

N

A

A

Y

I

G

G

I

H

E

P

L

L

S

T

V

L

I

G

N

Q

L

Q

I

L

L

V

I

V

T

V

T

L

T

T

V

A

V

V

A

L

A

A

S

S

I

I

G

G

T
|
T

P

A

A
x
G

I
x
V

P

P

G

G

G

A

G
|
G

V

A

I

I

I

M

L

L

A

A

S

M

V

V

L

L

Q

Q

S

S

A

V

G

G

I

L

P

D

I

L

D

T

-

P

-

G

-

S

-

P

-

V

-

A

-

L

G

A

L

Y

I

A

I

M

I

I

I

L

G

G

I

I

D

D

R

A

I

I

L

L

G
x
D

M

M

F

G

R
|
R

T
|
T

A

M

V

V

N
|
N

V

V

T

T

G

G

D

D

L

L

T

A

A

G

S

T

V

V

I

I

F

V

N

A

K

K

binding d-aspartic acid: R263 (≠ A294), S265 (= S296), M299 (= M330), T302 (= T333), T340 (= T371), G342 (≠ A373), V343 (≠ I374), G347 (= G378), D383 (≠ G407), R386 (= R410), T387 (= T411), N390 (= N414)
binding decyl-beta-d-maltopyranoside: H23 (≠ P42), V212 (≠ F243), A216 (≠ G247)

6bauA Crystal structure of gltph r397c in complex with l-cysteine (see paper)
34% identity, 91% coverage: 25:427/445 of query aligns to 7:406/408 of 6bauA

query
sites
6bauA

K

K

V

I

I

L

I

I

G

G

L

L

I

I

L

L

G

G

A

A

G

I

V

V

G

G

I

L

V

I

I

L

N

G

P

H

S

-

T

-

G

-

F

-

V

-

P

-

E

Y

N

G

F

Y

S

A

L

D

L

A

L

V

A

K

N

T

W

Y

L

V

D

K

L

P

P

F

G

G

Q

D

I

L

F

F

M

V

R

R

L

L

V

L

Q

K

M

M

I

L

M

V

I

M

P

P

L

I

I

V

F

F

A

A

S

S

I

L

I

V

S

V

G

G

I

A

V

A

G

S

N

I

T

S

S

P

E

A

N

R

L

L

K

G

A

R

F

V

G

G

I

V

K

K

L

I

L

V

Y

Y

F

Y

V

L

F

L

T

T

T

S

V

A

I

F

A

A

I

V

T

T

I

L

G

G

L

I

I

I

V

M

T

A

F

R

I

L

M

F

K

N

P

P

G

G

Q

A

Y

-

V

-

F

-

K

-

L

-

G

G

G

I

F

H

P

L

N

A

S

V

G

G

E

G

K

Q

L

Q

I

F

E

Q

A

P

N

K

E

Q

Q

A

T

P

N

P

L

L

I

V

E

K

N

I

I

L

P

L

D

D

T

-

I

-

S

-

K

-

L

I

I

V

P

P

N

T

N

N

P

P

L

F

E

G

S

A

I

L

L

A

M

N

G

G

E

Q

M

V

L

L

G

P

V

T

V

I

I

F

F

F

T

A

I

I

I

L

G

G

V

I

A

A

I

I

T

T

Q

Y

L

L

N

M

H

N

D

S

-

E

-

N

-

E

-

K

-

V

-

R

-

K

T

S

A

A

R

E

P

T

I

L

R

D

F

A

V

I

E

N

A

G

I

L

Q

A

K

E

I

A

C

M

M

Y

I

K

V

I

I

V

S

N

W

G

A

V

M

M

M

Q

L

Y

V

A

P

P

Y

I

A

G

V

V

F

F

G

A

L

L

I

I

A

A

A

Y

L

V

L

M

S

A

K

E

I

Q

G

G

I

V

E

K

I

V

F

V

L

G

G

E

L

L

G

A

Y

K

Y

V

M

T

I

A

V

A

V

V

I

Y

L

V

G

G

L

L

-

T

-

L

-

Q

I

I

L

L

M

V

I

Y