SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_024851134.1 NCBI__GCF_000526715.1:WP_024851134.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

2x5dD Crystal structure of a probable aminotransferase from pseudomonas aeruginosa (see paper)
71% identity, 96% coverage: 15:394/397 of query aligns to 1:380/380 of 2x5dD

query
sites
2x5dD

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active site: Y113 (= Y127), D191 (= D205), A193 (= A207), K225 (= K239)
binding pyridoxal-5'-phosphate: G87 (= G101), S88 (= S102), K89 (= K103), Y113 (= Y127), D191 (= D205), A193 (= A207), Y194 (= Y208), T222 (= T236), S224 (= S238), K225 (= K239), R233 (= R247)

6l1oB Product bound bacf structure from bacillus subtillis (see paper)
37% identity, 96% coverage: 8:388/397 of query aligns to 9:387/392 of 6l1oB

query
sites
6l1oB

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binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G103 (≠ S102), K104 (= K103), Y128 (= Y127), N174 (= N173), D206 (= D205), Y209 (= Y208), S236 (≠ T236), S238 (= S238), K239 (= K239), R247 (= R247)
binding tyrosine: F17 (= F16), Q39 (≠ M38), G40 (= G39), K104 (= K103), Y128 (= Y127), E130 (≠ I129), Y325 (≠ F324), R367 (= R368)

6l1lB Apo-bacf structure from bacillus subtillis (see paper)
37% identity, 96% coverage: 8:388/397 of query aligns to 9:387/393 of 6l1lB

query
sites
6l1lB

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binding pyridoxal-5'-phosphate: G103 (≠ S102), K104 (= K103), Y128 (= Y127), N174 (= N173), N178 (= N177), D206 (= D205), Y209 (= Y208), S236 (≠ T236), S238 (= S238), R247 (= R247)

6l1nA Substrate bound bacf structure from bacillus subtillis (see paper)
37% identity, 96% coverage: 8:388/397 of query aligns to 9:386/393 of 6l1nA

query
sites
6l1nA

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G

V

V

D

T

L

I

D

N

Y

P

E

E

T

T

E

E

A

V

V

A

V

L

T

F

I

G

G

G

S
x
G

K

K

E

A

G

G

L

L

A

Y

H

V

L

L

A

T

L

Q

A

C

T

L

V

L

E

N

K

P

G

G

D

D

T

I

V

A

L

L

V

V

P

P

N

N

P

P

A

G

Y
|
Y

P

P

I

E

H

Y

P

L

Y

S

G

G

F

I

V

T

I

M

A

A

G

R

A

A

D

E

I

L

R

Y

H

E

V

M

R

P

M

L

T

Y

P

E

D

E

V

N

D

G

F

Y

F

L

D

P

E

D

L

F

E

E

K

K

A

I

I

D

K

P

E

A

S

V

W

L

P

E

K

K

P

A

K

K

M

L

L

M

V

F

L

L

N
|
N

F

Y

P

P

G

N

N
|
N

P

P

T

T

T

G

Q

A

T

V

V

A

D

D

L

A

H

A

F

F

F

Y

E

A

K

K

V

A

V

A

A

A

I

F

A

A

K

K

E

E

H

H

N

N

I

I

W

H

V

L

I

I

H

H

D
|
D

L

F

A

A

Y
|
Y

A

G

D

A

I

F

A

E

F

F

D

D

G

-

Y

Q

K

K

A

P

P

A

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S

I

F

M

L

Q

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A

E

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G

D

A

A

K

K

D

T

I

V

A

G

V

A

E

E

F

L

Y

Y

T
x
S

L

F

S
|
S

K

K

S

T

Y

F

N

N

M

M

P

A

G

G

W

W

R
|
R

V

M

G

A

F

F

M

A

V

V

G

G

N

N

P

E

V

K

L

I

V

I

Q

Q

A

A

L

V

K

N

R

E

M

F

K

Q

S

D

Y

H

L

V

D

F

Y

V

G

G

T

M

F

F

T

G

P

G

I

L

Q

Q

V

Q

A

A

A

A

I

S

A

A

A

A

L

L

E

S

G

G

P

D

Q

P

D

E

C

H

V

T

Q

E

E

S

I

L

S

K

D

R

M

I

Y

Y

K

K

S

E

R

R

R

I

D

D

-

F

-

F

-

T

V

A

L

L

C

C

Q

E

G

-

L

-

N

K

S

E

I

L

G

G

W

W

K

K

V

M

E

E

P

K

P

P

K

K

A

G

T

T

M

F

F
x
Y

V

V

W

W

A

A

P

E

I

I

P

P

E

N

E

T

Y

F

R

E

D

T

M

-

G

-

S

S

L

H

E

Q

F

F

S

S

K

D

K

Y

L

L

L

L

T

E

D

H

A

A

K

H

V

V

A

V

V

V

A

T

P

P

G

G

I

E

G

I

F

F

G

G

D

S

Y

N

G

G

D

K

D

R

H

H

V

V

R
|
R

F

I

G

S

L

M

I

V

E

S

N

K

E

Q

H

E

R

D

T

L

R

R

Q

E

A

F

I

V

R

T

G

R

I

I

R

Q

D

K

M

L

binding glycine: E16 (≠ V15), Y127 (= Y127), N177 (= N177), Y324 (≠ F324), R366 (= R368)
binding pyridoxal-5'-phosphate: G102 (≠ S102), Y127 (= Y127), N173 (= N173), N177 (= N177), D205 (= D205), Y208 (= Y208), S235 (≠ T236), S237 (= S238), R246 (= R247)

2o1bA Structure of aminotransferase from staphylococcus aureus
32% identity, 88% coverage: 33:381/397 of query aligns to 21:364/376 of 2o1bA

query
sites
2o1bA

I

L

I

I

D

N

F

M

G

A

M

V

G

G

N

I

P

P

D

D

Q

G

D

P

T

T

P

P

K

Q

H

G

I

I

V

I

D

D

K

H

L

F

I

Q

E

K

V

A

V

L

Q

T

R

I

E

P

G

E

T

N

H

Q

R

K

Y

Y

S

G

V

A

S

F

Q

H

G

G

I

K

P

E

R

A

L

F

R

K

R

Q

A

A

I

I

C

V

N

D

W

F

Y

Y

K

Q

T

R

K

Q

F

Y

D

N

V

V

D

T

L

L

D

D

Y

K

E

E

T

D

E

E

A

V

V

C

V

I

T

L

I

Y

G

G

S
x
T

K
|
K

E

N

G

G

L

L

A

V

H

A

L

V

A

P

L

T

A

C

T

V

V

I

E

N

K

P

G

G

D

D

T

Y

V

V

L

L

V

L

P

P

N

D

P

P

A

G

Y
|
Y

P

T

I

D

H

Y

P

L

Y

A

G

G

F

V

V

L

I

L

A

A

G

D

A

G

D

K

I

P

R

V

H

P

V

L

R

N

M

L

T

E

P

P

D

P

V

-

D

H

F

Y

F

L

D

P

E

D

L

W

E

S

K

K

A

V

I

D

K

S

E

Q

S

I

W

I

P

D

K

K

P

T

K

K

M

L

L

I

V

Y

L

L

N

T

F

Y

P

P

G

N

N
|
N

P

P

T

T

T

G

Q

S

T

T

V

A

D

T

L

K

H

E

F

V

F

F

E

D

K

E

V

A

V

I

A

A

I

K

A

F

K

K

E

G

H

T

N

D

I

T

W

K

V

I

I

V

H

H

D
|
D

L

F

A
|
A

Y
|
Y

A

G

D

A

I

F

A

G

F

F

D

D

G

A

Y

-

K

K

A

N

P

P

S

S

I

I

M

L

Q

A

V

S

E

E

G

N

A

G

K

K

D

D

I

V

A

A

V

I

E

E

F

I

Y

Y

T
x
S

L

L

S
|
S

K
|
K

S

G

Y

Y

N

N

M

M

P

S

G

G

W

F

R
|
R

V

V

G

G

F

F

M

A

V

V

G

G

N

N

P

K

V

D

L

M

V

I

Q

Q

A

A

L

L

K

K

R

K

M

Y

K

Q

S

T

Y

H

L

T

D

N

Y

A

G

G

T

M

F

F

T

G

P

A

I

L

Q

Q

V

D

A

A

A

A

I

I

A

Y

A

A

L

L

E

N

G

H

P

Y

Q

D

D

D

C

F

V

L

Q

E

E

E

I

Q

S

S

D

N

M

V

Y

F

K

K

S

T

R

R

R

R

D

D

V

R

L

F

C

E

Q

A

G

M

L

L

N

A

S

K

I

A

G

D

W

L

K

P

V

F

E

V

P

H

P

A

K

K

A

G

T

G

M

I

F

Y

V

V

W

W

A

L

P

E

I

T

P

P

E

P

E

G

Y

Y

R

D

D

-

M

-

G

-

S

S

L

E

E

Q

F

F

S

E

K

Q

K

F

L

L

L

V

T

Q

D

E

A

K

K

S

V

I

A

L

V

V

A

A

P

P

G

G

I

K

G

P

F

F

G

G

D

E

Y

N

G

G

D

N

D

R

H

Y

V

V

R

R

F

I

G

S

L

L

I

A

E

L

N

D

E

D

H

Q

R

K

T

L

R

D

Q

E

A

A

active site: Y115 (= Y127), D192 (= D205), A194 (= A207), K225 (= K239)
binding pyridoxal-5'-phosphate: T90 (≠ S102), K91 (= K103), Y115 (= Y127), N164 (= N177), D192 (= D205), A194 (= A207), Y195 (= Y208), S222 (≠ T236), S224 (= S238), K225 (= K239), R233 (= R247)

1j32A Aspartate aminotransferase from phormidium lapideum
32% identity, 91% coverage: 7:369/397 of query aligns to 6:364/388 of 1j32A

query
sites
1j32A

R

R

I

V

K

E

R

S

L

V

P

S

P

P

Y

S

V

M

F

T

N

L

I

I

T

I

G

D

E

A

L

K

K

A

A

K

E

A

A

M

R

K

R

A

R

E

G

G

E

I

D

D

I

V

I

C

D

S

F

F

G

S

M

A

G

G

N

E

P

P

D

D

Q

F

D

N

T

T

P

P

K

K

H

H

I

I

V

V

D

E

K

A

L

A

I

K

E

A

V

A

V

L

Q

E

R

-

E

Q

G

G

T

K

H

T

R

R

Y

Y

S

G

V

P

S

A

Q

A

G

G

I

E

P

P

R

R

L

L

R

R

R

E

A

A

I

I

C

A

N

Q

W

-

Y

-

K

K

T

L

K

Q

F

R

D

D

V

N

D

G

L

L

D

C

Y

Y

E

G

T

A

E

D

A

N

V

I

-

L

V

V

T

T

I

N

G
|
G

S
x
G

K
|
K

E

Q

G

S

L

I

A

F

H

N

L

L

A

M

L

L

A

A

T

M

V

I

E

E

K

P

G

G

D

D

T

E

V

V

L

I

V

I

P

P

N

A

P

P

A

F

Y
x
W

P

V

I

S

H

Y

P

P

Y

E

G

M

F

V

V

K

I

L

A

A

G

E

A

G

D

T

I

P

R

V

H

I

V

L

R

P

M

T

T

T

P

V

D

E

V

T

D

Q

F

F

F

K

D

V

E

S

L

P

E

E

K

Q

A

I

I

R

K

Q

E

A

S

I

W

T

P

P

K

K

P

T

K

K

M

L

L

L

V

V

L

F

N

N

F

T

P

P

G

S

N
|
N

P

P

T

T

T

G

Q

M

T

V

V

Y

D

T

L

P

H

D

F

E

F

V

E

R

K

A

V

I

V

A

A

Q

I

V

A

A

K

V

E

E

H

A

N

G

I

L

W

W

V

V

I

L

H

S

D
|
D

L

E

A
x
I

Y
|
Y

A

E

D

K

I

I

A

L

F

Y

D

D

G

D

Y

A

K

Q

A

H

P

L

S

S

I

I

M

G

Q

A

V

A

E

S

-

P

G

E

A

A

K

Y

D

E

I

R

A

S

V

V

E

V

F

C

Y

S

T

G

L

F

S

A

K
|
K

S

T

Y

Y

N

A

M

M

P

T

G

G

W

W

R
|
R

V

V

G

G

F

F

M

L

V

A

G

G

N

P

P

V

V

P

L

L

V

V

Q

K

A

A

L

A

K

T

R

K

M

I

K

Q

S

G

Y

H

L

S

D

T

Y

S

G

N

T

V

F

C

T

T

P

F

I

A

Q

Q

V

Y

A

G

A

A

I

I

A

A

A

A

L

Y

E

E

G

N

P

S

Q

Q

D

D

C

C

V

V

Q

Q

E

E

I

M

S

L

D

A

M

A

Y

F

K

A

S

E

R

R

R

R

D

R

V

Y

L

M

C

L

Q

D

G

A

L

L

N

N

S

A

I

M

-

P

G

G

W

L

K

E

V

C

E

P

P

K

P

P

K

D

A

G

T

A

M

F

F

Y

V

M

W

F

A

P

P

S

I

I

P

A

E

K

E

T

Y

G

R

R

D

S

M

-

G

-

S

S

L

L

E

D

F

F

S

C

K

S

K

E

L

L

L

L

T

D

D

Q

A

H

K

Q

V

V

A

A

V

T

A

V

P

P

G

G

I

A

G

A

F

F

G

G

D

-

Y

-

G

A

D

D

D

D

H

C

V

I

R

R

F

L

active site: W124 (≠ Y127), D202 (= D205), I204 (≠ A207), K237 (= K239)
binding pyridoxal-5'-phosphate: G98 (= G101), G99 (≠ S102), K100 (= K103), W124 (≠ Y127), N174 (= N177), D202 (= D205), I204 (≠ A207), Y205 (= Y208), K237 (= K239), R245 (= R247)

Q58097 (5-formylfuran-3-yl)methyl phosphate transaminase; 4-HFC-P:alanine aminotransferase; EC 2.6.1.108 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
29% identity, 93% coverage: 22:391/397 of query aligns to 20:370/370 of Q58097

query
sites
Q58097

L

L

K

A

A

Q

E

K

A

L

R

E

R

S

R

E

G

G

E

K

D

K

I

V

I

I

D

H

F

L

G

E

M

I

G

G

N

E

P

P

D

D

Q

F

D

N

T

T

P

P

K

K

H

P

I

I

V

V

D

D

K

E

L

G

I

I

E

K

V

S

V

L

Q

-

R

K

E

E

G

G

T

K

H

T

R

H

Y

Y

S

T

V

D

S

S

Q

R

G

G

I

I

P

L

R

E

L

L

R

R

R

E

A

K

I

I

C

S

N

E

W

L

Y

Y

K

K

T

D

K

K

F

Y

D

K

V

A

D

D

L

I

D

-

Y

I

E

P

T

D

E

N

A

I

V

I

V

I

T

T

I

G

G

G

S

S

K

S

E

L

G

G

L

L

A

F

H

F

L

A

A

L

L

S

A

S

T

I

V

I

E

D

K

D

G

G

D

D

T

E

V

V

L

L

V

I

P

Q

N

N

P

P

A

C

Y

Y

P

P

I

C

H

Y

P

K

Y

N

G

F

F

I

V

R

I

F

A

L

G

G

A

A

D

K

I

P

R

V

H

F

V

C

R

D

M

F

T

T

P

-

D

-

V

-

D

-

F

-

F

V

D

E

E

S

L

L

E

E

K

E

A

A

I

L

K

S

E

D

S

-

W

-

P

-

K

K

P

T

K

K

M

A

L

I

V

I

L

I

N

N

F

S

P

P

G

S

N

N

P

P

T

L

T

G

Q

E

T

V

V

I

D

D

L

R

H

E

F

I

F

Y

E

E

K

-

V

-

V

-

A

-

I

F

A

A

K

Y

E

E

H

N

N

I

I

P

W

Y

V

I

I

I

H

S

D

D

L

E

A

I

Y

Y

A

N

D

G

I

L

A

V

F

Y

D

E

G

G

Y

-

K

K

A

C

P

Y

S

S

I

A

M

I

Q

E

V

F

E

D

G

E

A

N

K

L

D

E

I

K

A

T

V

I

E

L

F

I

Y

N

T

G

L

F

S

S

K
|
K

S

L

Y

Y

N

A

M

M

P

T

G

G

W

W

R

R

V

I

G

G

F

Y

M

V

V

I

G

S

N

N

P

D

V

E

L

I

V

I

Q

E

A

A

L

I

K

L

R

K

M

L

K

Q

S

Q

Y

N

L

L

D

F

Y

I

G

S

T

A

F

P

T

T

P

I

I

S

Q

Q

V

Y

A

A

A

A

I

L

A

K

A

A

L

F

E

E

G

-

P

-

Q

K

D

E

C

T

V

E

Q

R

E

E

I

I

S

N

D

S

M

M

Y

I

K

K

S

E

-

F

-

D

-

R

R

R

R

R

D

R

V

L

L

V

C

L

Q

K

G

Y

L

V

N

K

S

D

I

F

G

G

W

W

K

E

V

V

E

N

P

N

P

P

K

I

A

G

T

A

M

Y

F

Y

V

V

W

F

A

P

P

N

I

I

P

G

E

E

E

D

Y

G

R

R

D

-

M

-

G

-

S

-

L

-

E

E

F

F

S

A

K

Y

K

K

L

L

L

L

T

K

D

E

A

K

K

F

V

V

A

A

V

L

A

T

P

P

G

G

I

I

G

G

F

F

G

G

D

S

Y

K

G

G

D

K

D

N

H

Y

V

I

R

R

F

I

G

S

L

Y

I

A

E

N

N

S

E

Y

H

E

R

N

T

I

R

K

Q

E

A

G

I

L

R

E

G

R

I

I

R

K

D

E

M

F

F

L

R

N

K

K

K222 (= K239) modified: N6-(pyridoxal phosphate)lysine

1gdeA Crystal structure of pyrococcus protein a-1 e-form (see paper)
29% identity, 92% coverage: 31:395/397 of query aligns to 25:388/388 of 1gdeA

query
sites
1gdeA

E

K

D

D

I

V

I

I

D

S

F

L

G

G

M

I

G

G

N

E

P

P

D

D

Q

F

D

D

T

T

P

P

K

Q

H

H

I

I

V

K

D

E

K

Y

L

A

I

K

E

E

V

A

V

L

Q

D

R

K

E

G

G

L

T

T

H

H

R

-

Y

Y

S

G

V

P

S

N

Q

I

G

G

I

L

P

L

R

E

L

L

R

R

R

E

A

A

I

I

C

A

N

E

W

K

Y

L

K

K

T

K

K

Q

F

N

D

G

V

I

D

E

L

A

D

D

Y

P

E

K

T

T

E

E

A

I

V

M

V

V

T

L

I

L

G
|
G

S
x
A

K
x
N

E

Q

G

A

L

F

A

L

H

M

L

G

A

L

L

S

A

A

T

F

V

L

E

K

K

D

G

G

D

E

T

E

V

V

L

L

V

I

P

P

N

T

P

P

A

A

Y
x
F

P

V

I

S

H

Y

P

A

Y

P

G

A

F

V

V

I

I

L

A

A

G

G

A

G

D

K

I

P

R

V

H

E

V

V

R

P

M

T

T

Y

P

E

D

E

V

D

D

E

F

F

-

R

-

L

-

N

F

V

D

D

E

E

L

L

E

K

K

K

A

Y

I

V

K

T

E

D

S

-

W

-

P

-

K

K

P

T

K

R

M

A

L

L

V

I

L

I

N
|
N

F

S

P

P

G

C

N
|
N

P

P

T

T

T

G

Q

A

T

V

V

L

D

T

L

K

H

K

F

D

F

L

E

E

K

E

V

I

V

A

A

D

I

F

A

V

K

V

E

E

H

H

N

D

I

L

W

I

V

V

I

I

H

S

D
|
D

L

E

A

V

Y
|
Y

A

E

D

H

I

F

A

I

F

Y

D

D

G

D

Y

A

K

R

A

H

P

Y

S

S

I

I

M

A

Q

S

V

L

E

D

G

G

A

M

K

F

D

E

I

R

A

T

V

I

E

T

F

V

Y

N

T

G

L

F

S
|
S

K
|
K

S

T

Y

F

N

A

M

M

P

T

G

G

W

W

R
|
R

V

L

G

G

F

F

M

V

V

A

G

A

N

P

P

S

V

W

L

I

V

I

Q

E

A

R

L

M

K

V

R

K

M

F

K

Q

S

M

Y

Y

L

N

D

A

Y

T

G

C

T

P

F

V

T

T

P

F

I

I

Q

Q

V

Y

A

A

A

A

I

A

A

K

A

A

L

L

-

K

-

D

E

E

G

R

P

S

Q

W

D

K

C

A

V

V

Q

E

E

E

I

M

S

R

D

K

M

E

Y

Y

K

D

S

R

R

R

R

R

D

K

V

L

L

V

C

W

Q

K

G

R

L

L

N

N

S

E

I

M

G

G

W

L

K

P

V

T

E

V

P

K

P

P

K

K

A

G

T

A

M

F

F

Y

V

I

W

F

A

P

P

R

I

I

P

-

E

-

E

-

Y

-

R

R

D

D

M

T

G

G

-

L

-

T

S

S

L

K

E

K

F

F

S

S

K

E

K

L

L

M

L

L

T

K

D

E

A

A

K

R

V

V

A

A

V

V

A

V

P

P

G

G

I

S

G

A

F

F

G

G

D

K

Y

A

G

G

D

E

D

G

H

Y

V

V

R
|
R

F

I

G

S

L

Y

I

A

E

T

N

A

E

Y

H

E

R

K

T

L

R

E

Q

E

A

A

I

M

R

D

G

R

I

M

R

E

D

R

M

V

F

L

R

K

K

E

D

R

G

K

L

L

I

V

active site: K232 (= K239)
binding glutamic acid: F120 (≠ Y127), N170 (= N177), R361 (= R368)
binding pyridoxal-5'-phosphate: G94 (= G101), A95 (≠ S102), N96 (≠ K103), F120 (≠ Y127), N166 (= N173), D198 (= D205), Y201 (= Y208), S231 (= S238), K232 (= K239), R240 (= R247)

1gd9A Crystall structure of pyrococcus protein-a1 (see paper)
29% identity, 92% coverage: 31:395/397 of query aligns to 25:388/388 of 1gd9A

query
sites
1gd9A

E

K

D

D

I

V

I

I

D

S

F

L

G

G

M

I

G

G

N

E

P

P

D

D

Q

F

D

D

T

T

P

P

K

Q

H

H

I

I

V

K

D

E

K

Y

L

A

I

K

E

E

V

A

V

L

Q

D

R

K

E

G

G

L

T

T

H

H

R

-

Y

Y

S

G

V

P

S

N

Q

I

G

G

I

L

P

L

R

E

L

L

R

R

R

E

A

A

I

I

C

A

N

E

W

K

Y

L

K

K

T

K

K

Q

F

N

D

G

V

I

D

E

L

A

D

D

Y

P

E

K

T

T

E

E

A

I

V

M

V

V

T

L

I

L

G
|
G

S
x
A

K
x
N

E

Q

G

A

L

F

A

L

H

M

L

G

A

L

L

S

A

A

T

F

V

L

E

K

K

D

G

G

D

E

T

E

V

V

L

L

V

I

P

P

N

T

P

P

A

A

Y
x
F

P

V

I

S

H

Y

P

A

Y

P

G

A

F

V

V

I

I

L

A

A

G

G

A

G

D

K

I

P

R

V

H

E

V

V

R

P

M

T

T

Y

P

E

D

E

V

D

D

E

F

F

-

R

-

L

-

N

F

V

D

D

E

E

L

L

E

K

K

K

A

Y

I

V

K

T

E

D

S

-

W

-

P

-

K

K

P

T

K

R

M

A

L

L

V

I

L

I

N

N

F

S

P

P

G

C

N
|
N

P

P

T

T

T

G

Q

A

T

V

V

L

D

T

L

K

H

K

F

D

F

L

E

E

K

E

V

I

V

A

A

D

I

F

A

V

K

V

E

E

H

H

N

D

I

L

W

I

V

V

I

I

H

S

D
|
D

L

E

A
x
V

Y
|
Y

A

E

D

H

I

F

A

I

F

Y

D

D

G

D

Y

A

K

R

A

H

P

Y

S

S

I

I

M

A

Q

S

V

L

E

D

G

G

A

M

K

F

D

E

I

R

A

T

V

I

E

T

F

V

Y

N

T

G

L

F

S
|
S

K
|
K

S

T

Y

F

N

A

M

M

P

T

G

G

W

W

R
|
R

V

L

G

G

F

F

M

V

V

A

G

A

N

P

P

S

V

W

L

I

V

I

Q

E

A

R

L

M

K

V

R

K

M

F

K

Q

S

M

Y

Y

L

N

D

A

Y

T

G

C

T

P

F

V

T

T

P

F

I

I

Q

Q

V

Y

A

A

A

A

I

A

A

K

A

A

L

L

-

K

-

D

E

E

G

R

P

S

Q

W

D

K

C

A

V

V

Q

E

E

E

I

M

S

R

D

K

M

E

Y

Y

K

D

S

R

R

R

R

R

D

K

V

L

L

V

C

W

Q

K

G

R

L

L

N

N

S

E

I

M

G

G

W

L

K

P

V

T

E

V

P

K

P

P

K

K

A

G

T

A

M

F

F

Y

V

I

W

F

A

P

P

R

I

I

P

-

E

-

E

-

Y

-

R

R

D

D

M

T

G

G

-

L

-

T

S

S

L

K

E

K

F

F

S

S

K

E

K

L

L

M

L

L

T

K

D

E

A

A

K

R

V

V

A

A

V

V

A

V

P

P

G

G

I

S

G

A

F

F

G

G

D

K

Y

A

G

G

D

E

D

G

H

Y

V

V

R

R

F

I

G

S

L

Y

I

A

E

T

N

A

E

Y

H

E

R

K

T

L

R

E

Q

E

A

A

I

M

R

D

G

R

I

M

R

E

D

R

M

V

F

L

R

K

K

E

D

R

G

K

L

L

I

V

active site: K232 (= K239)
binding pyridoxal-5'-phosphate: G94 (= G101), A95 (≠ S102), N96 (≠ K103), F120 (≠ Y127), N170 (= N177), D198 (= D205), V200 (≠ A207), Y201 (= Y208), S231 (= S238), K232 (= K239), R240 (= R247)

Q56232 Aspartate/prephenate aminotransferase; AspAT / PAT; Transaminase A; EC 2.6.1.1; EC 2.6.1.78 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see 3 papers)
29% identity, 96% coverage: 1:382/397 of query aligns to 1:375/385 of Q56232

query
sites
Q56232

M

M

T

R

G

G

D

L

F

S

E

R

R

R

I

V

K

Q

R

A

L

M

P
x
K

P

P

Y

S

V

A

F

T

N

V

I

A

T

V

G

N

E

A

L

K

K

A

A

L

E

E

A

L

R

R

R

R

R

Q

G

G

E

V

D

D

I

L

I

V

D

A

F

L

G

T

M

A

G

G

N

E

P

P

D

D

Q

F

D

D

T

T

P

P

K

E

H

H

I

V

V

K

D

E

K

A

L

A

I

R

E

R

V

A

V

L

Q

A

R

-

E

Q

G

G

T

K

H

T

R

K

Y

Y

S

A

V

P

S

P

Q

A

G

G

I

I

P

P

R

E

L

L

R

R

R

E

A

A

I

L

C

A

N

E

W

K

Y

F

K

R

T

R

K

E

F

N

D

G

V

L

D

S

L

V

D

T

Y

P

E

E

T

-

E

E

A

T

V

I

V

V

T

T

I

V

G

G

S

G

K

K

E

Q

G

A

L

L

A

F

H

N

L

L

A

F

L

Q

A

A

T

I

V

L

E

D

K

P

G

G

D

D

T

E

V

V

L

I

V

V

P

L

N

S

P

P

A

Y

Y

W

P

V

I

S

H

Y

P

P

Y

E

G

M

F

V

V

R

I

F

A

A

G

G

A

G

D

V

I

V

R

V

H

E

V

V

R

E

M

T

T

L

P

P

D

E

V

E

D

G

F

F

F

V

D

P

E

D

L

P

E

E

K

R

A

V

I

R

K

R

E

A

S

I

W

T

P

P

K

R

P

T

K

K

M

A

L

L

V

V

L

V

N

N

F

S

P

P

G

N

N

N

P

P

T

T

T

G

Q

A

T

V

V

Y

D

P

L

K

H

E

F

V

F

L

E

E

K

A

V

L

V

A

A

R

I

L

A

A

K

V

E

E

H

H

N

D

I

F

W

Y

V

L

I

V

H

S

D

D

L

E

A

I

Y

Y

A

E

D

H

I

L

A

L

F

Y

D

E

G

G

Y

E

K

H

-

F

-

S

-

P

-

G

-

R

-

V

A

A

P

P

S

E

-

H

I

T

M

L

Q

T

V

V

E

N

G

G

A

A

K

-

D

-

I

-

A

-

V

-

E

-

F

-

Y

-

T

-

L

-

S

A

K
|
K

S

A

Y

F

N

A

M

M

P

T

G

G

W

W

R

R

V

I

G

G

F

Y

M

A

V

C

G

G

N

P

P

K

V

E

L

V

V

I

Q

K

A

A

L

M

K

A

R

S

M

V

K

S

S

S

Y

Q

L

S

D

T

Y

T

G

S

T

P

F

D

T

T

P

I

I

A

Q

Q

V

W

A

A

A

T

I

L

A

E

A

A

L

L

-

T

-

N

-

Q

E

E

G

A

P

S

Q

R

D

A

C

F

V

V

Q

E

E

M

I

A

S

R

D

E

M

A

Y

Y

K

R

S

R

R

R

R

R

D

D

V

L

L

L

C

L

Q

E

G

G

L

L

N

T

S

A

I

L

G

G

W

L

K

K

V

A

E

V

P

R

P

P

K

S

A

G

T

A

M

F

F

Y

V

V

W

L

-

M

-

D

-

T

A

S

P

P

I

I

-

A

P

P

E

D

E

E

Y

V

R

R

D

-

M

-

G

-

S

-

L

-

E

-

F

-

S

A

K

A

K

E

L

R

L

L

T

L

D

E

A

A

K

G

V

V

A

A

V

V

A

V

P

P

G

G

I

T

G

D

F

F

G

A

D

A

Y

F

G

G

D

-

D

-

H

H

V

V

R

R

F

L

G

S

L

Y

I

A

E

T

N

S

E

E

H

E

R

N

T

L

R

R

Q

K

A

A

I

L

K12 (≠ P12) Important for prephenate aminotransferase activity; mutation to G: 10-fold increase in Km for prephenate. Does not affect Km for oxaloacetate.
K234 (= K239) modified: N6-(pyridoxal phosphate)lysine

1bkgA Aspartate aminotransferase from thermus thermophilus with maleate (see paper)
29% identity, 96% coverage: 1:382/397 of query aligns to 1:375/382 of 1bkgA

query
sites
1bkgA

M

M

T

R

G

G

D

L

F

S

E

R

R

R

I

V

K

Q

R

A

L

M

P

K

P

P

Y

S

V

A

F

T

N

V

I

A

T

V

G

N

E

A

L

K

K

A

A

L

E

E

A

L

R

R

R

R

R

Q

G

G

E

V

D

D

I

L

I

V

D

A

F

L

G

T

M

A

G

G

N

E

P

P

D

D

Q

F

D

D

T

T

P

P

K

E

H

H

I

V

V

K

D

E

K

A

L

A

I

R

E

R

V

A

V

L

Q

A

R

-

E

Q

G

G

T

K

H

T

R

K

Y

Y

S

A

V

P

S

P

Q

A

G

G

I

I

P

P

R

E

L

L

R

R

R

E

A

A

I

L

C

A

N

E

W

K

Y

F

K

R

T

R

K

E

F

N

D

G

V

L

D

S

L

V

D

T

Y

P

E

E

T

-

E

E

A

T

V

I

V

V

T

T

I

V

G
|
G

S
x
G

K
|
K

E

Q

G

A

L

L

A

F

H

N

L

L

A

F

L

Q

A

A

T

I

V

L

E

D

K

P

G

G

D

D

T

E

V

V

L

I

V

V

P

L

N

S

P

P

A

Y

Y
x
W

P

V

I

S

H

Y

P

P

Y

E

G

M

F

V

V

R

I

F

A

A

G

G

A

G

D

V

I

V

R

V

H

E

V

V

R

E

M

T

T

L

P

P

D

E

V

E

D

G

F

F

F

V

D

P

E

D

L

P

E

E

K

R

A

V

I

R

K

R

E

A

S

I

W

T

P

P

K

R

P

T

K

K

M

A

L

L

V

V

L

V

N
|
N

F

S

P

P

G

N

N
|
N

P

P

T

T

T

G

Q

A

T

V

V

Y

D

P

L

K

H

E

F

V

F

L

E

E

K

A

V

L

V

A

A

R

I

L

A

A

K

V

E

E

H

H

N

D

I

F

W

Y

V

L

I

V

H

S

D
|
D

L

E

A
x
I

Y
|
Y

A

E

D

H

I

L

A

L

F

Y

D

E

G

G

Y

E

K

H

-

F

-

S

-

P

-

G

-

R

-

V

A

A

P

P

S

E

-

H

I

T

M

L

Q

T

V

V

E

N

G

G

A

A

K

-

D

-

I

-

A

-

V

-

E

-

F

-

Y

-

T

-

L

-

S

A

K
|
K

S

A

Y

F

N

A

M

M

P

T

G

G

W

W

R
|
R

V

I

G

G

F

Y

M

A

V

C

G

G

N

P

P

K

V

E

L

V

V

I

Q

K

A

A

L

M

K

A

R

S

M

V

K

S

S

S

Y

Q

L

S

D

T

Y

T

G

S

T

P

F

D

T

T

P

I

I

A

Q

Q

V

W

A

A

A

T

I

L

A

E

A

A

L

L

-

T

-

N

-

Q

E

E

G

A

P

S

Q

R

D

A

C

F

V

V

Q

E

E

M

I

A

S

R

D

E

M

A

Y

Y

K

R

S

R

R

R

R

R

D

D

V

L

L

L

C

L

Q

E

G

G

L

L

N

T

S

A

I

L

G

G

W

L

K

K

V

A

E

V

P

R

P

P

K

S

A

G

T

A

M

F

F

Y

V

V

W

L

-

M

-

D

-

T

A

S

P

P

I

I

-

A

P

P

E

D

E

E

Y

V

R

R

D

-

M

-

G

-

S

-

L

-

E

-

F

-

S

A

K

A

K

E

L

R

L

L

T

L

D

E

A

A

K

G

V

V

A

A

V

V

A

V

P

P

G

G

I

T

G

D

F

F

G

A

D

A

Y

F

G

G

D

-

D

-

H

H

V

V

R

R

F

L

G

S

L

Y

I

A

E

T

N

S

E

E

H

E

R

N

T

L

R

R

Q

K

A

A

I

L

active site: W125 (≠ Y127), D203 (= D205), I205 (≠ A207), K234 (= K239)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G99 (= G101), G100 (≠ S102), K101 (= K103), W125 (≠ Y127), N171 (= N173), N175 (= N177), D203 (= D205), I205 (≠ A207), Y206 (= Y208), K234 (= K239), R242 (= R247)

1bjwA Aspartate aminotransferase from thermus thermophilus (see paper)
29% identity, 96% coverage: 1:382/397 of query aligns to 1:375/382 of 1bjwA

query
sites
1bjwA

M

M

T

R

G

G

D

L

F

S

E

R

R

R

I

V

K

Q

R

A

L

M

P

K

P

P

Y

S

V

A

F

T

N

V

I

A

T

V

G

N

E

A

L

K

K

A

A

L

E

E

A

L

R

R

R

R

R

Q

G

G

E

V

D

D

I

L

I

V

D

A

F

L

G

T

M

A

G
|
G

N

E

P

P

D

D

Q

F

D

D

T

T

P

P

K

E

H

H

I

V

V

K

D

E

K

A

L

A

I

R

E

R

V

A

V

L

Q

A

R

-

E

Q

G

G

T

K

H

T

R

K

Y

Y

S

A

V

P

S

P

Q

A

G

G

I

I

P

P

R

E

L

L

R

R

R

E

A

A

I

L

C

A

N

E

W

K

Y

F

K

R

T

R

K

E

F

N

D

G

V

L

D

S

L

V

D

T

Y

P

E

E

T

-

E

E

A

T

V

I

V

V

T

T

I

V

G

G

S

G

K

K

E

Q

G

A

L

L

A

F

H

N

L

L

A

F

L

Q

A

A

T

I

V

L

E

D

K

P

G

G

D

D

T

E

V

V

L

I

V

V

P

L

N

S

P

P

A

Y

Y
x
W

P

V

I

S

H

Y

P

P

Y

E

G

M

F

V

V

R

I

F

A

A

G

G

A

G

D

V

I

V

R

V

H

E

V

V

R

E

M

T

T

L

P

P

D

E

V

E

D

G

F

F

F

V

D

P

E

D

L

P

E

E

K

R

A

V

I

R

K

R

E

A

S

I

W

T

P

P

K

R

P

T

K

K

M

A

L

L

V

V

L

V

N

N

F

S

P

P

G

N

N
|
N

P

P

T

T

T

G

Q

A

T

V

V

Y

D

P

L

K

H

E

F

V

F

L

E

E

K

A

V

L

V

A

A

R

I

L

A

A

K

V

E

E

H

H

N

D

I

F

W

Y

V

L

I

V

H

S

D
|
D

L

E

A
x
I

Y

Y

A

E

D

H

I

L

A

L

F

Y

D

E

G

G

Y

E

K

H

-

F

-

S

-

P

-

G

-

R

-

V

A

A

P

P

S

E

-

H

I

T

M

L

Q

T

V

V

E

N

G

G

A

A

K

-

D

-

I

-

A

-

V

-

E

-

F

-

Y

-

T

-

L

-

S

A

K
|
K

S

A

Y

F

N

A

M

M

P

T

G

G

W

W

R

R

V

I

G

G

F

Y

M

A

V

C

G

G

N

P

P

K

V

E

L

V

V

I

Q

K

A

A

L

M

K

A

R

S

M

V

K

S

S

S

Y

Q

L

S

D

T

Y

T

G

S

T

P

F

D

T

T

P

I

I

A

Q

Q

V

W

A

A

A

T

I

L

A

E

A

A

L

L

-

T

-

N

-

Q

E

E

G

A

P

S

Q

R

D

A

C

F

V

V

Q

E

E

M

I

A

S

R

D

E

M

A

Y

Y

K

R

S

R

R

R

R

R

D

D

V

L

L

L

C

L

Q

E

G

G

L

L

N

T

S

A

I

L

G

G

W

L

K

K

V

A

E

V

P

R

P

P

K

S

A

G

T

A

M

F

F

Y

V

V

W

L

-

M

-

D

-

T

A

S

P

P

I

I

-

A

P

P

E

D

E

E

Y

V

R

R

D

-

M

-

G

-

S

-

L

-

E

-

F

-

S

A

K

A

K

E

L

R

L

L

T

L

D

E

A

A

K

G

V

V

A

A

V

V

A

V

P

P

G

G

I

T

G

D

F

F

G

A

D

A

Y

F

G

G

D

-

D

-

H

H

V

V

R
|
R

F

L

G

S

L

Y

I

A

E

T

N

S

E

E

H

E

R

N

T

L

R

R

Q

K

A

A

I

L

active site: W125 (≠ Y127), D203 (= D205), I205 (≠ A207), K234 (= K239)
binding phosphate ion: G39 (= G39), W125 (≠ Y127), N175 (= N177), R361 (= R368)

1b5oA Thermus thermophilus aspartate aminotransferase single mutant 1 (see paper)
28% identity, 96% coverage: 1:382/397 of query alig