SitesBLAST
Comparing WP_024852149.1 NCBI__GCF_000526715.1:WP_024852149.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
8wm7C Cryo-em structure of cyanobacterial nitrate/nitrite transporter nrtbcd in complex with signalling protein pii (see paper)
44% identity, 90% coverage: 25:261/263 of query aligns to 23:267/658 of 8wm7C
Sites not aligning to the query:
8w9mC Cryo-em structure of the cyanobacterial nitrate transporter nrtbcd in complex with atp (see paper)
49% identity, 78% coverage: 25:230/263 of query aligns to 23:232/256 of 8w9mC
- binding adenosine-5'-triphosphate: S42 (= S44), G43 (= G45), G45 (= G47), K46 (= K48), S47 (= S49), T48 (= T50), Q83 (= Q85), K132 (= K131), E136 (= E135), S138 (= S137), G140 (= G139), H195 (= H194)
- binding magnesium ion: S47 (= S49), Q83 (= Q85)
Sites not aligning to the query:
8wm7D Cryo-em structure of cyanobacterial nitrate/nitrite transporter nrtbcd in complex with signalling protein pii (see paper)
43% identity, 85% coverage: 24:246/263 of query aligns to 23:257/257 of 8wm7D
Sites not aligning to the query:
8w9mD Cryo-em structure of the cyanobacterial nitrate transporter nrtbcd in complex with atp (see paper)
43% identity, 85% coverage: 24:246/263 of query aligns to 21:255/256 of 8w9mD
- binding adenosine-5'-triphosphate: H40 (≠ R43), S41 (= S44), G42 (= G45), G44 (= G47), K45 (= K48), S46 (= S49), T47 (= T50), Q82 (= Q85), Q135 (≠ E135), S137 (= S137), G139 (= G139), M140 (≠ Q140), H194 (= H194)
- binding magnesium ion: S46 (= S49), Q82 (= Q85)
Sites not aligning to the query:
2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
42% identity, 81% coverage: 24:236/263 of query aligns to 17:233/374 of 2awnB
Sites not aligning to the query:
P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
42% identity, 81% coverage: 24:236/263 of query aligns to 18:234/371 of P68187
- A85 (≠ S88) mutation to M: Suppressor of EAA loop mutations in MalFG.
- K106 (≠ Y109) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V114 (= V116) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V117 (≠ L119) mutation to M: Suppressor of EAA loop mutations in MalFG.
- E119 (≠ D121) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- A124 (≠ S126) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G137 (= G139) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
- D158 (= D160) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
- R228 (= R230) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
Sites not aligning to the query:
- 241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 267 W→G: Normal maltose transport but constitutive mal gene expression.
- 278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
- 322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 340 G→A: Maltose transport is affected but retains ability to interact with MalT.
- 346 G→S: Normal maltose transport but constitutive mal gene expression.
- 355 F→Y: Maltose transport is affected but retains ability to interact with MalT.
1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
42% identity, 81% coverage: 24:236/263 of query aligns to 15:231/367 of 1q12A
- binding adenosine-5'-triphosphate: S35 (= S44), G36 (= G45), C37 (= C46), G38 (= G47), K39 (= K48), S40 (= S49), T41 (= T50), R126 (≠ K131), A130 (≠ E135), S132 (= S137), G134 (= G139), Q135 (= Q140)
Sites not aligning to the query:
3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
42% identity, 81% coverage: 24:236/263 of query aligns to 17:233/371 of 3puyA
- binding phosphoaminophosphonic acid-adenylate ester: S37 (= S44), G38 (= G45), C39 (= C46), G40 (= G47), K41 (= K48), S42 (= S49), T43 (= T50), Q81 (= Q85), R128 (≠ K131), A132 (≠ E135), S134 (= S137), G136 (= G139), Q137 (= Q140), E158 (= E161), H191 (= H194)
- binding magnesium ion: S42 (= S49), Q81 (= Q85)
Sites not aligning to the query:
3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
42% identity, 81% coverage: 24:236/263 of query aligns to 17:233/371 of 3puxA
- binding adenosine-5'-diphosphate: G38 (= G45), C39 (= C46), G40 (= G47), K41 (= K48), S42 (= S49), T43 (= T50), R128 (≠ K131), S134 (= S137), Q137 (= Q140)
- binding beryllium trifluoride ion: S37 (= S44), G38 (= G45), K41 (= K48), Q81 (= Q85), S134 (= S137), G136 (= G139), H191 (= H194)
- binding magnesium ion: S42 (= S49), Q81 (= Q85)
Sites not aligning to the query:
3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
42% identity, 81% coverage: 24:236/263 of query aligns to 17:233/371 of 3puwA
- binding adenosine-5'-diphosphate: V17 (= V24), G38 (= G45), C39 (= C46), G40 (= G47), K41 (= K48), S42 (= S49), T43 (= T50), R128 (≠ K131), A132 (≠ E135), S134 (= S137), Q137 (= Q140)
- binding tetrafluoroaluminate ion: S37 (= S44), G38 (= G45), K41 (= K48), Q81 (= Q85), S134 (= S137), G135 (= G138), G136 (= G139), E158 (= E161), H191 (= H194)
- binding magnesium ion: S42 (= S49), Q81 (= Q85)
Sites not aligning to the query:
3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
42% identity, 81% coverage: 24:236/263 of query aligns to 17:233/371 of 3puvA
- binding adenosine-5'-diphosphate: V17 (= V24), G38 (= G45), C39 (= C46), G40 (= G47), K41 (= K48), S42 (= S49), T43 (= T50), R128 (≠ K131), A132 (≠ E135), S134 (= S137), Q137 (= Q140)
- binding magnesium ion: S42 (= S49), Q81 (= Q85)
Sites not aligning to the query:
P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
43% identity, 81% coverage: 24:236/263 of query aligns to 18:234/369 of P19566
- L86 (= L89) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- P160 (= P162) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- D165 (= D167) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
Sites not aligning to the query:
- 306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.
2awnC Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
42% identity, 81% coverage: 24:236/263 of query aligns to 10:203/344 of 2awnC
2d62A Crystal structure of multiple sugar binding transport atp-binding protein
38% identity, 81% coverage: 25:236/263 of query aligns to 22:243/375 of 2d62A
1g291 Malk (see paper)
37% identity, 81% coverage: 25:236/263 of query aligns to 19:240/372 of 1g291
- binding magnesium ion: D69 (≠ K75), E71 (≠ S77), K72 (≠ A78), K79 (vs. gap), D80 (vs. gap)
- binding pyrophosphate 2-: S38 (= S44), G39 (= G45), C40 (= C46), G41 (= G47), K42 (= K48), T43 (≠ S49), T44 (= T50)
Sites not aligning to the query:
P9WQI3 Trehalose import ATP-binding protein SugC; MtbSugC; Nucleotide-binding domain of SugABC transporter; NBD of SugABC transporter; SugABC transporter ATPase SugC; EC 7.5.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
39% identity, 80% coverage: 27:236/263 of query aligns to 22:235/393 of P9WQI3
- H193 (= H194) mutation to A: Decreased hydrolysis of ATP. No change in KM, but 2-fold reduction in Vmax compared to wild-type.
1oxvD Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
39% identity, 77% coverage: 8:209/263 of query aligns to 4:214/353 of 1oxvD
1oxvA Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
39% identity, 77% coverage: 8:209/263 of query aligns to 4:214/353 of 1oxvA
1oxuA Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
39% identity, 77% coverage: 8:209/263 of query aligns to 4:214/353 of 1oxuA
Q97UY8 Glucose import ATP-binding protein GlcV; EC 7.5.2.- from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see paper)
39% identity, 77% coverage: 8:209/263 of query aligns to 4:214/353 of Q97UY8