SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_025272809.1 NCBI__GCF_000527155.1:WP_025272809.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

P69783 PTS system glucose-specific EIIA component; EIIA-Glc; EIII-Glc; Glucose-specific phosphotransferase enzyme IIA component from Escherichia coli (strain K12) (see 6 papers)
41% identity, 80% coverage: 3:121/148 of query aligns to 22:140/169 of P69783

query
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P69783

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H76 (= H57) Important for phospho-donor activity; binding Zn(2+); mutation to Q: Unable to transfer phosphoryl group.
H91 (= H72) active site, Tele-phosphohistidine intermediate; for EIIA activity; binding Zn(2+); modified: Phosphohistidine; by HPr; mutation to Q: Unable to be phosphorylated by Hpr.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
4 F→W: Same activity as the wild-type.

1glcF Cation promoted association (cpa) of a regulatory and target protein is controlled by phosphorylation (see paper)
41% identity, 80% coverage: 3:121/148 of query aligns to 14:132/161 of 1glcF

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1glcF

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binding zinc ion: H68 (= H57), H83 (= H72)

1o2fA Complex of enzyme iiaglc and iibglc phosphocarrier protein hpr from escherichia coli nmr, restrained regularized mean structure (see paper)
41% identity, 80% coverage: 3:121/148 of query aligns to 3:121/150 of 1o2fA

query
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1o2fA

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active site: T55 (vs. gap), H57 (= H57), H72 (= H72)
binding phosphite ion: H72 (= H72), D76 (= D76)

P09323 PTS system N-acetylglucosamine-specific EIICBA component; EIICBA-Nag; EII-Nag; EC 2.7.1.193 from Escherichia coli (strain K12) (see paper)
40% identity, 82% coverage: 1:122/148 of query aligns to 498:619/648 of P09323

query
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P09323

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Sites not aligning to the query:

1 modified: N-formylmethionine

Query Sequence

>WP_025272809.1 NCBI__GCF_000527155.1:WP_025272809.1
MAEVLAPVAGDITPLDQVSDPVFAQGFVGPGLAVTPVAEPTTAVAAIGGTVTKLHPHAYV
ITGDDGRGVLVHLGIDTVELKGEGFTLLAAEGDTVTAGQAIVEWNPAEIKAGGKDTVCPV
VALDGQPDAITDHATGEVSAGDRLFTWQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory