SitesBLAST
Comparing WP_025273295.1 NCBI__GCF_000527155.1:WP_025273295.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
A6QDA0 N-(2-amino-2-carboxyethyl)-L-glutamate synthase; ACEGA synthase; Staphyloferrin B biosynthesis protein SbnA; EC 2.5.1.140 from Staphylococcus aureus (strain Newman) (see paper)
37% identity, 92% coverage: 15:309/321 of query aligns to 21:316/326 of A6QDA0
- K47 (= K41) modified: N6-(pyridoxal phosphate)lysine
- N77 (= N71) binding pyridoxal 5'-phosphate
- R132 (= R126) mutation to A: No detectable enzyme activity. Does not form pyridoxal 5'-phosphate-alpha-aminoacrylate reaction intermediate.
- Y152 (= Y146) mutation to F: Very low enzyme activity. Does not form pyridoxal 5'-phosphate-alpha-aminoacrylate reaction intermediate; when associated with G-185.
- STTGS 185:189 (≠ GTSGT 178:182) binding pyridoxal 5'-phosphate
- S272 (= S265) binding pyridoxal 5'-phosphate
5d85A Staphyloferrin b precursor biosynthetic enzyme sbna bound to aminoacrylate intermediate (see paper)
37% identity, 92% coverage: 15:309/321 of query aligns to 13:308/313 of 5d85A
- active site: K39 (= K41), S264 (= S265)
- binding citrate anion: S67 (= S69), K92 (≠ N94), G119 (= G121), Y120 (≠ F122), L121 (= L123), R124 (= R126), R216 (= R217), A223 (= A224), S224 (= S225)
- binding 2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]acrylic acid: K39 (= K41), T66 (≠ S68), S67 (= S69), N69 (= N71), L70 (= L72), Q143 (= Q145), V176 (≠ T177), S177 (≠ G178), T178 (= T179), T179 (≠ S180), S181 (≠ T182), G220 (= G221), S264 (= S265), P290 (= P291), D291 (= D292)
5d84A Staphyloferrin b precursor biosynthetic enzyme sbna bound to plp (see paper)
37% identity, 92% coverage: 15:309/321 of query aligns to 15:310/318 of 5d84A
- active site: K41 (= K41), S266 (= S265)
- binding pyridoxal-5'-phosphate: K41 (= K41), N71 (= N71), V178 (≠ T177), S179 (≠ G178), T180 (= T179), T181 (≠ S180), S183 (≠ T182), G222 (= G221), S266 (= S265), P292 (= P291), D293 (= D292)
5d86A Staphyloferrin b precursor biosynthetic enzyme sbna y152f variant (see paper)
36% identity, 92% coverage: 15:309/321 of query aligns to 14:309/318 of 5d86A
- active site: K40 (= K41), S265 (= S265)
- binding magnesium ion: S225 (= S225), R226 (= R226)
- binding pyridoxal-5'-phosphate: K40 (= K41), N70 (= N71), V177 (≠ T177), S178 (≠ G178), T179 (= T179), T180 (≠ S180), S182 (≠ T182), G221 (= G221), S265 (= S265), P291 (= P291), D292 (= D292)
8if7A Crystal structure of cmnb (see paper)
37% identity, 88% coverage: 25:308/321 of query aligns to 36:319/342 of 8if7A
- binding 2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]acrylic acid: K52 (= K41), T78 (≠ S68), S79 (= S69), N81 (= N71), L82 (= L72), V188 (≠ T177), S189 (≠ G178), T190 (= T179), G191 (≠ S180), T193 (= T182), G232 (= G221), S276 (= S265), A302 (≠ P291), D303 (= D292)
8srvB Crystal structure of o-acetyl-l-serine sulfhydrylase a (cysk) from staphylococcus aureus nctc 8325 complexed with a transcriptional repressor (cymr) derived 10 amino acid peptide
31% identity, 93% coverage: 4:303/321 of query aligns to 13:313/314 of 8srvB
- binding : T79 (≠ S68), S80 (= S69), G81 (= G70), T83 (≠ L72), S127 (≠ P116), A129 (≠ S118), M130 (≠ T119), Q151 (= Q145), F152 (≠ Y146), S215 (= S207), G224 (≠ K215), G230 (= G221), G232 (= G223), A233 (= A224), G234 (≠ S225)
Q79FV4 S-sulfocysteine synthase; O-phospho-L-serine-dependent S-sulfocysteine synthase; OPS-dependent S-sulfocysteine synthase; O-phosphoserine sulfhydrylase; EC 2.8.5.1; EC 2.5.1.65 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
31% identity, 90% coverage: 23:310/321 of query aligns to 48:333/372 of Q79FV4
- R243 (≠ P220) mutation to A: 10-fold decrease in the specificity constant for thiosulfate compared to wild-type enzyme but little change in the specificity constant for the substrate OPS.
2bhtA Crystal structure of o-acetylserine sulfhydrylase b (see paper)
32% identity, 92% coverage: 6:300/321 of query aligns to 4:289/294 of 2bhtA
- active site: K41 (= K41), S69 (= S69), Q199 (≠ D203), G203 (≠ S207), S255 (= S265), C280 (≠ S290)
- binding pyridoxal-5'-phosphate: K41 (= K41), N71 (= N71), M173 (vs. gap), G174 (= G178), T175 (= T179), T176 (≠ S180), T178 (= T182), G208 (≠ P214), S255 (= S265), C280 (≠ S290)
2efyA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 4-acetylbutyric acid
35% identity, 86% coverage: 26:300/321 of query aligns to 25:298/302 of 2efyA
- active site: K40 (= K41), S70 (= S69), E200 (≠ D203), S204 (≠ G206), S263 (= S265)
- binding 5-oxohexanoic acid: T69 (≠ S68), G71 (= G70), T73 (≠ L72), Q141 (= Q145), G175 (= G178), G219 (= G221), M220 (≠ L222), P222 (≠ A224)
- binding pyridoxal-5'-phosphate: K40 (= K41), N72 (= N71), Y172 (≠ V175), G175 (= G178), T176 (= T179), G177 (≠ S180), T179 (= T182), G219 (= G221), S263 (= S265), P289 (= P291), D290 (= D292)
2ecqA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 3-hydroxylactate
35% identity, 86% coverage: 26:300/321 of query aligns to 25:298/302 of 2ecqA
- active site: K40 (= K41), S70 (= S69), E200 (≠ D203), S204 (≠ G206), S263 (= S265)
- binding (3s)-3-hydroxybutanoic acid: K40 (= K41), G71 (= G70), T73 (≠ L72), Q141 (= Q145), G219 (= G221)
- binding pyridoxal-5'-phosphate: K40 (= K41), N72 (= N71), Y172 (≠ V175), G173 (= G176), G175 (= G178), T176 (= T179), T179 (= T182), G219 (= G221), S263 (= S265), P289 (= P291)
2ecoA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 4-methylvalerate
35% identity, 86% coverage: 26:300/321 of query aligns to 25:298/302 of 2ecoA
- active site: K40 (= K41), S70 (= S69), E200 (≠ D203), S204 (≠ G206), S263 (= S265)
- binding 4-methyl valeric acid: K40 (= K41), T69 (≠ S68), G71 (= G70), T73 (≠ L72), Q141 (= Q145), G175 (= G178), T176 (= T179), G219 (= G221)
- binding pyridoxal-5'-phosphate: K40 (= K41), N72 (= N71), Y172 (≠ V175), G175 (= G178), T176 (= T179), T179 (= T182), G219 (= G221), S263 (= S265), P289 (= P291), D290 (= D292)
P16703 Cysteine synthase B; CSase B; O-acetylserine (thiol)-lyase B; OAS-TL B; O-acetylserine sulfhydrylase B; EC 2.5.1.47 from Escherichia coli (strain K12) (see paper)
31% identity, 95% coverage: 6:309/321 of query aligns to 4:299/303 of P16703
- N71 (= N71) binding pyridoxal 5'-phosphate
- S255 (= S265) binding pyridoxal 5'-phosphate
5xoqA Crystal structure of o-acetylserine sulfhydrylase with bound transcription factor peptide inhibitor from planctomyces limnophilus
31% identity, 93% coverage: 2:300/321 of query aligns to 4:303/310 of 5xoqA
- binding : T72 (≠ S68), S73 (= S69), G74 (= G70), T76 (≠ L72), M123 (≠ T119), Q144 (= Q145), R218 (= R216), H219 (≠ R217), Q222 (≠ P220), G223 (= G221), A226 (= A224)
3bm5A Crystal structure of o-acetyl-serine sulfhydrylase from entamoeba histolytica in complex with cysteine (see paper)
32% identity, 86% coverage: 26:300/321 of query aligns to 42:315/338 of 3bm5A
4jbnA Crystal structure of o-acetyl serine sulfhydrylase from entamoeba histolytica in complex with serine acetyl transferase derived tetrapeptide, spsi (see paper)
32% identity, 86% coverage: 26:300/321 of query aligns to 42:315/336 of 4jbnA
4il5A Crystal structure of o-acetyl serine sulfhydrylase from entamoeba histolytica in complex with isoleucine (see paper)
32% identity, 86% coverage: 26:300/321 of query aligns to 42:315/336 of 4il5A
4jblB Crystal structure of o-acetyl serine sulfhydrylase from entamoeba histolytica in complex with methionine (see paper)
32% identity, 86% coverage: 26:300/321 of query aligns to 42:315/335 of 4jblB
- active site: K57 (= K41), S279 (= S265)
- binding methionine: K57 (= K41), T84 (≠ S68), S85 (= S69), N87 (= N71), T88 (≠ L72), M135 (≠ T119), Q158 (= Q145), F159 (≠ Y146), G191 (= G178), G235 (= G221), I236 (≠ L222), A238 (= A224)
3bm5B Crystal structure of o-acetyl-serine sulfhydrylase from entamoeba histolytica in complex with cysteine (see paper)
32% identity, 86% coverage: 26:300/321 of query aligns to 42:315/335 of 3bm5B
- active site: K57 (= K41), S279 (= S265)
- binding cysteine: K57 (= K41), T84 (≠ S68), S85 (= S69), T88 (≠ L72), G235 (= G221)
- binding pyridoxal-5'-phosphate: K57 (= K41), N87 (= N71), G191 (= G178), T192 (= T179), S193 (= S180), T195 (= T182), G235 (= G221), S279 (= S265), P306 (= P291)
6kr5B Crystal structure of o-acetyl serine sulfhydrylase isoform 3 from entamoeba histolytica (see paper)
31% identity, 86% coverage: 26:300/321 of query aligns to 41:314/336 of 6kr5B
- binding pyridoxal-5'-phosphate: K56 (= K41), N86 (= N71), V189 (≠ T177), G190 (= G178), T191 (= T179), A192 (≠ S180), T194 (= T182), G234 (= G221), S278 (= S265), P305 (= P291), D306 (= D292)
4lmbA Crystal structure analysis of o-acetylserine sulfhydrylase cysk2 complexed with cystine from microcystis aeruginosa 7806 (see paper)
33% identity, 93% coverage: 1:300/321 of query aligns to 2:305/310 of 4lmbA
- active site: K46 (= K41), S269 (= S265)
- binding cysteine: K46 (= K41), T74 (≠ S68), S75 (= S69), N77 (= N71), T78 (≠ L72), M101 (≠ V95), M125 (≠ L123), M125 (≠ L123), Q147 (= Q145), F148 (≠ Y146), Q224 (≠ P220), G225 (= G221), G225 (= G221), I226 (≠ L222), A228 (= A224)
- binding pyridoxal-5'-phosphate: K46 (= K41), N77 (= N71), V180 (≠ T177), G181 (= G178), T182 (= T179), G183 (≠ S180), T185 (= T182), G225 (= G221), S269 (= S265), P296 (= P291)
Query Sequence
>WP_025273295.1 NCBI__GCF_000527155.1:WP_025273295.1
MLYNDVSEIITDDIFVNLKDFLPQRQVFLKLEGLNPSGSIKAKAAASLLEDAEKSGRLGP
GKKIIESSSGNLGIALSTACAAKGYPLTIVTDANVQESALRTMRALGTRLTIIERPDSTG
GFLHQRIDYIHRSLNDDPDLVWLNQYSNPANVWAHTATTAHAVDNELGPIDALFVGTGTS
GTLMGCLEFRRTHRRRHTIVAVDSEGSVTFGGPPKRRYIPGLGASRKPEIYSEAESFDKV
LIPEADTIAECHRLARTYGLLAGGSTGTVLAAIRKYGRRFPPGARIASISPDMGEKYLPT
VYSHEWLSRHPDLIDKETTSV
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory