SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_025273295.1 NCBI__GCF_000527155.1:WP_025273295.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

A6QDA0 N-(2-amino-2-carboxyethyl)-L-glutamate synthase; ACEGA synthase; Staphyloferrin B biosynthesis protein SbnA; EC 2.5.1.140 from Staphylococcus aureus (strain Newman) (see paper)
37% identity, 92% coverage: 15:309/321 of query aligns to 21:316/326 of A6QDA0

query
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A6QDA0

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K47 (= K41) modified: N6-(pyridoxal phosphate)lysine
N77 (= N71) binding pyridoxal 5'-phosphate
R132 (= R126) mutation to A: No detectable enzyme activity. Does not form pyridoxal 5'-phosphate-alpha-aminoacrylate reaction intermediate.
Y152 (= Y146) mutation to F: Very low enzyme activity. Does not form pyridoxal 5'-phosphate-alpha-aminoacrylate reaction intermediate; when associated with G-185.
STTGS 185:189 (≠ GTSGT 178:182) binding pyridoxal 5'-phosphate
S272 (= S265) binding pyridoxal 5'-phosphate

5d85A Staphyloferrin b precursor biosynthetic enzyme sbna bound to aminoacrylate intermediate (see paper)
37% identity, 92% coverage: 15:309/321 of query aligns to 13:308/313 of 5d85A

query
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active site: K39 (= K41), S264 (= S265)
binding citrate anion: S67 (= S69), K92 (≠ N94), G119 (= G121), Y120 (≠ F122), L121 (= L123), R124 (= R126), R216 (= R217), A223 (= A224), S224 (= S225)
binding 2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]acrylic acid: K39 (= K41), T66 (≠ S68), S67 (= S69), N69 (= N71), L70 (= L72), Q143 (= Q145), V176 (≠ T177), S177 (≠ G178), T178 (= T179), T179 (≠ S180), S181 (≠ T182), G220 (= G221), S264 (= S265), P290 (= P291), D291 (= D292)

5d84A Staphyloferrin b precursor biosynthetic enzyme sbna bound to plp (see paper)
37% identity, 92% coverage: 15:309/321 of query aligns to 15:310/318 of 5d84A

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active site: K41 (= K41), S266 (= S265)
binding pyridoxal-5'-phosphate: K41 (= K41), N71 (= N71), V178 (≠ T177), S179 (≠ G178), T180 (= T179), T181 (≠ S180), S183 (≠ T182), G222 (= G221), S266 (= S265), P292 (= P291), D293 (= D292)

5d86A Staphyloferrin b precursor biosynthetic enzyme sbna y152f variant (see paper)
36% identity, 92% coverage: 15:309/321 of query aligns to 14:309/318 of 5d86A

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active site: K40 (= K41), S265 (= S265)
binding magnesium ion: S225 (= S225), R226 (= R226)
binding pyridoxal-5'-phosphate: K40 (= K41), N70 (= N71), V177 (≠ T177), S178 (≠ G178), T179 (= T179), T180 (≠ S180), S182 (≠ T182), G221 (= G221), S265 (= S265), P291 (= P291), D292 (= D292)

8if7A Crystal structure of cmnb (see paper)
37% identity, 88% coverage: 25:308/321 of query aligns to 36:319/342 of 8if7A

query
sites
8if7A

R

R

Q

T

V

L

F

L

L

L

K

K

L

L

E

E

G

G

L

R

N

S

P

P

S

W

G

R

S

S

I

I

K
|
K

A

G

K

R

A

T

A

A

A

L

S

G

L

L

L

V

E

R

D

S

A

I

E

A

K

P

S

-

G

-

R

R

L

M

G

A

P

S

G

R

K

D

-

V

K

T

I

V

E

E

S

S

S
x
T

S
|
S

G

G

N
|
N

L
|
L

G

G

I

V

A

A

L

L

S

S

T

A

A

I

C

C

A

R

A

D

K

L

G

G

Y

L

P

P

L

F

T

V

I

A

V

V

T

V

D

D

A

L

N

K

V

Q

Q

S

E

P

S

V

A

I

L

Q

R

A

T

A

M

I

R

E

A

A

L

N

G

G

T

A

R

R

L

L

T

E

I

V

E

R

R

T

P

P

D

A

S

A

T

A

G

T

F

H

L

L

H

L

Q

D

R

R

I

L

D

D

Y

R

I

V

H

R

R

K

S

L

L

V

N

A

D

E

D

I

P

P

D

G

L

A

V

V

W

W

L

P

N

N

Q

Q

Y

Y

S

E

N

N

P

D

A

A

N

N

V

R

W

H

A

V

H

H

T

E

A

T

T

W

T

T

A

A

H

P

A

E

V

I

D

D

N

R

E

Q

L

V

G

G

-

G

P

E

I

A

D

Q

A

A

L

V

F

F

V

V

G

A

T
x
V

G
x
S

T
|
T

S
x
G

G

G

T
|
T

L

L

M

A

G

G

C

L

L

A

E

A

F

H

R

F

R

R

T

R

H

A

R

R

R

P

R

A

H

T

T

R

I

L

V

V

A

A

V

V

D

D

S

V

E

E

G

G

S

S

V

T

T

V

F

F

G

G

P

V

P

P

K

G

R

G

R

R

Y

V

I

L

P

T

G
|
G

L

I

G

G

A

A

S

S

R

R

K

R

P

S

E

T

I

F

Y

L

S

T

E

R

A

A

E

E

S

C

F

D

D

D

K

L

V

V

L

Y

I

V

P

R

E

E

A

A

D

A

T

A

I

I

A

A

E

A

C

C

H

H

R

V

L

L

A

R

R

A

T

D

Y

T

G

G

L

I

L

A

A

V

G

G

S
|
S

T

S

G

G

T

A

V

V

L

V

A

A

A

G

I

A

R

L

K

D

Y

H

G

L

R

A

F

H

P

P

P

G

G

L

A

T

R

T

I

A

A

V

S

C

I

V

S

C

P
x
A

D
|
D

M

L

G

G

E

E

K

N

Y

Y

L

A

P

R

T

T

V

V

Y

Y

S

D

H

P

E

D

W

W

L

L

S

A

binding 2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]acrylic acid: K52 (= K41), T78 (≠ S68), S79 (= S69), N81 (= N71), L82 (= L72), V188 (≠ T177), S189 (≠ G178), T190 (= T179), G191 (≠ S180), T193 (= T182), G232 (= G221), S276 (= S265), A302 (≠ P291), D303 (= D292)

8srvB Crystal structure of o-acetyl-l-serine sulfhydrylase a (cysk) from staphylococcus aureus nctc 8325 complexed with a transcriptional repressor (cymr) derived 10 amino acid peptide
31% identity, 93% coverage: 4:303/321 of query aligns to 13:313/314 of 8srvB

query
sites
8srvB

N

D

D

N

V

I

S

T

E

Q

I

I

T

G

D

G

D

T

I

P

F

V

V

V

N

K

L

L

K

R

D

N

F

V

L

V

P

D

Q

D

R

N

-

A

Q

D

V

V

F

Y

L

V

K

K

L

L

E

E

G

Y

L

Q

N

N

P

P

S

G

G

G

S

S

I

V

K

K

A

D

K

R

A

I

A

A

A

L

S

A

L

M

L

I

E

E

D

K

A

A

E

E

K

R

S

E

G

G

R

K

L

I

G

K

P

P

G

G

K

D

K

T

I

I

I

V

E

E

S

P

S
x
T

S
|
S

G
|
G

N

N

L
x
T

G

G

I

I

A

G

L

L

S

A

T

F

A

V

C

C

A

A

K

K

G

G

Y

Y

P

K

L

A

T

V

I

F

V

T

T

M

D

P

A

E

N

T

V

M

Q

S

E

Q

S

E

A

R

L

R

R

N

T

L

M

L

R

K

A

A

L

Y

G

G

T

A

R

E

L

L

T

V

I

L

I

T

E

P

R

G

P
x
S

D

E

S
x
A

T
x
M

G

K

G

G

F

A

L

I

H

K

Q

K

R

-

I

-

D

-

Y

-

I

-

H

A

R

K

S

E

L

L

N

K

D

E

P

H

D

G

L

Y

V

F

W

E

L

P

N

Q

Q
|
Q

Y
x
F

S

E

N

N

P

P

A

A

N

N

V

P

W

E

A

V

H

H

T

E

A

L

T

T

A

G

H

P

A

E

V

L

D

L

N

Q

E

Q

L

F

-

E

-

G

G

K

P

T

I

I

D

D

A

A

L

F

F

L

V

A

G

G

T

V

G

G

T

T

S

G

G

G

T

T

L

L

M

S

G

G

C

V

L

G

E

K

F

V

R

L

R

K

T

K

H

E

R

Y

R

P

R

N

H

I

T

E

I

I

V

V

A

A

V

I

D

E

S

P

E

E

G

A

S
|
S

-

P

V

V

T

L

F

S

G

G

P

E

P

P

K
x
G

R

P

R

H

Y

K

I

L

P

Q

G
|
G

L

L

G
|
G

A
|
A

S
x
G

R

F

K

I

P

P

E

G

I

T

Y

L

S

N

E

-

A

T

E

E

S

I

F

Y

D

D

K

S

V

I

L

I

I

K

P

V

E

G

A

N

D

D

T

T

I

A

A

M

E

E

-

M

C

S

H

R

R

R

L

V

A

A

R

K

T

E

Y

E

G

G

L

I

L

L

A

A

G

G

G

I

S

S

T

S

G

G

T

A

V

A

L

I

A

Y

A

A

I

A

R

I

K

Q

Y

K

G

A

R

K

R

E

F

L

P

G

P

K

G

G

A

K

R

T

I

V

A

V

S

T

I

V

S

L

P

P

D

S

M

N

G

G

E

E

K

R

Y

Y

L

L

P

S

T

T

-

P

V

L

Y

Y

S

S

binding : T79 (≠ S68), S80 (= S69), G81 (= G70), T83 (≠ L72), S127 (≠ P116), A129 (≠ S118), M130 (≠ T119), Q151 (= Q145), F152 (≠ Y146), S215 (= S207), G224 (≠ K215), G230 (= G221), G232 (= G223), A233 (= A224), G234 (≠ S225)

Q79FV4 S-sulfocysteine synthase; O-phospho-L-serine-dependent S-sulfocysteine synthase; OPS-dependent S-sulfocysteine synthase; O-phosphoserine sulfhydrylase; EC 2.8.5.1; EC 2.5.1.65 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
31% identity, 90% coverage: 23:310/321 of query aligns to 48:333/372 of Q79FV4

query
sites
Q79FV4

P

P

Q

D

R

R

Q

G

V

F

F

W

L

A

K

K

L

L

E

E

G

G

L

F

N

N

P

P

S

G

G

G

S

-

I

M

K

K

A

D

K

R

A

P

A

A

A

L

S

Y

L

M

L

V

E

E

D

C

A

A

E

R

K

A

S

R

G

G

R

D

L

I

G

A

P

P

G

G

K

A

I

I

I

V

E

E

S

S

S

T

S

G

G

G

N

T

L

L

G

G

I

L

A

G

L

L

S

A

T

L

A

A

C

G

A

K

A

V

K

Y

G

R

Y

H

P

P

L

V

T

T

I

L

V

V

T

T

D

D

A

P

N

G

V

L

Q

E

E

P

S

I

A

I

L

A

R

R

T

M

M

L

R

T

A

A

L

Y

G

G

T

A

R

G

L

V

T

D

I

M

I

V

E

T

R

Q

P

P

D

H

S

P

T

V

G

G

F

W

L

Q

H

Q

Q

A

R

R

I

K

D

D

Y

R

I

V

H

A

R

Q

S

L

L

M

N

A

D

E

D

Y

P

P

D

G

L

A

V

W

W

N

L

P

N

N

Q

Q

Y

Y

S

G

N

N

P

P

A

D

N

N

V

V

W

G

A

A

H

Y

T

R

A

S

T

L

T

A

A

L

H

E

A

L

V

V

D

-

N

A

E

Q

L

L

G

G

P

R

I

I

D

D

A

V

L

L

F

V

V

C

G

S

T

V

G

G

T

T

S

G

G

G

T

H

L

S

M

A

G

G

C

V

L

A

E

R

F

V

R

L

R

R

T

E

H

F

R

N

R

P

R

D

H

M

T

R

I

L

V

I

A

G

V

V

D

D

S

T

E

I

G

G

S

S

V

T

T

I

F

F

G

G

G

Q

P

P

P

A

K

S

R

N

R

R

Y

L

I

M

P
x
R

G

G

L

L

G

G

A

S

S

S

R

I

K

Y

P

P

E

R

I

-

Y

N

S

V

E

D

A

Y

E

R

S

A

F

F

D

D

K

E

V

V

-

H

L

W

I

V

P

A

E

P

A

P

D

E

T

A

I

V

A

W

E

A

C

C

H

R

R

S

L

L

A

A

R

A

T

T

Y

H

G

Y

L

A

L

S

A

G

G

G

G

W

S

S

T

V

G

G

T

A

V

V

L

A

A

L

A

V

I

A

R

G

K

W

Y

A

G

A

R

R

R

N

F

L

P

P

P

A

G

D

A

T

R

T

I

I

A

A

S

A

I

V

S

F

P

P

D

D

M

G

G

P

E

Q

K

R

Y

Y

L

F

P

D

T

T

V

I

Y

Y

S

N

H

D

E

A

W

Y

L

C

S

N

R

E

H

H

R243 (≠ P220) mutation to A: 10-fold decrease in the specificity constant for thiosulfate compared to wild-type enzyme but little change in the specificity constant for the substrate OPS.

2bhtA Crystal structure of o-acetylserine sulfhydrylase b (see paper)
32% identity, 92% coverage: 6:300/321 of query aligns to 4:289/294 of 2bhtA

query
sites
2bhtA

V

L

S

E

E

Q

I

T

I

I

T

G

D

N

D

T

I

P

F

L

V

V

N

K

L

L

K

Q

D

R

F

M

L

G

P

P

Q

D

R

N

-

G

-

S

Q

E

V

V

F

W

L

L

K

K

L

L

E

E

G

G

L

N

N

N

P

P

S

A

G

G

S

S

I

V

K
|
K

A

D

K

R

A

A

A

L

S

S

L

M

L

I

E

V

D

E

A

A

E

E

K

K

S

R

G

G

R

R

L

I

G

K

P

P

G

G

K

D

K

V

I

L

I

I

E

E

S

A

S

T

S
|
S

G

G

N
|
N

L

T

G

G

I

I

A

A

L

L

S

A

T

M

A

I

C

A

A

A

A

L

K

K

G

G

Y

Y

P

R

L

M

T

K

I

L

V

L

T

M

D

P

A

D

N

N

V

M

Q

S

E

Q

S

E

A

R

L

R

R

A

T

A

M

M

R

R

A

A

L

Y

G

G

T

A

R

E

L

L

T

I

I

L

I

V

E

T

R

K

P

E

D

Q

S

G

T

M

G

E

G

G

F

A

L

R

H

D

Q

L

R

A

I

L

D

E

Y

M

I

A

H

N

R

R

S

G

L

E

N

G

D

K

D

-

P

-

D

-

L

-

V

-

W

L

L

L

N

D

Q

Q

Y

F

S

N

N

N

P

P

A

D

N

N

V

P

W

K

A

A

H

H

T

Y

A

T

T

T

A

G

H

P

A

E

V

I

D

W

N

Q

E

Q

L

T

G

G

P

G

I

R

D

I

A

T

L

H

F

F

V

V

G

S

T

S

-
x
M

G
|
G

T
|
T

S
x
T

G

G

T
|
T

L

I

M

T

G

G

C

V

L

S

E

E

F

F

R

M

R

R

T

E

H

Q

R

S

R

K

R

P

H

V

T

T

I

I

V

V

A

G

V

L

D
x
Q

S

P

E

E

G

E

S
x
G

V

S

T

S

F

I

G

-

P

P

P
x
G

K

I

R

R

-

W

-

P

-

T

-

E

Y

Y

I

L

P

P

G

G

L

I

G

-

A

-

S

-

R

-

K

-

P

-

E

-

I

-

Y

-

S

F

E

N

A

A

E

S

S

L

F

V

D

D

K

E

V

V

L

L

-

D

I

I

P

H

E

Q

A

R

D

D

T

A

I

E

A

N

E

T

C

M

H

R

R

E

L

L

A

A

R

V

T

R

Y

E

G

G

L

I

L

F

A

C

G

G

G

V

S
|
S

T

S

G

G

T

G

V

A

L

V

A

A

A

G

I

A

R

L

K

R

Y

V

G

A

R

K

R

A

F

N

P

P

P

D

G

A

A

V

R

V

I

V

A

A

S

I

I

I

S
x
C

P

-

D

D

M

R

G

G

E

D

K

R

Y

Y

L

L

P

S

T

T

active site: K41 (= K41), S69 (= S69), Q199 (≠ D203), G203 (≠ S207), S255 (= S265), C280 (≠ S290)
binding pyridoxal-5'-phosphate: K41 (= K41), N71 (= N71), M173 (vs. gap), G174 (= G178), T175 (= T179), T176 (≠ S180), T178 (= T182), G208 (≠ P214), S255 (= S265), C280 (≠ S290)

2efyA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 4-acetylbutyric acid
35% identity, 86% coverage: 26:300/321 of query aligns to 25:298/302 of 2efyA

query
sites
2efyA

Q

E

V

V

F

W

L

V

K

K

L

L

E

E

G

G

L

L

N

N

P

P

S

G

G

G

S

S

I

I

K
|
K

A

D

K

R

A

P

A

A

A

W

S

Y

L

M

L

I

E

K

D

D

A

A

E

E

K

E

S

R

G

G

-

I

-

L

R

R

L

P

G

G

P

S

G

G

K

Q

K

V

I

I

I

V

E

E

S

P

S
x
T

S
|
S

G
|
G

N
|
N

L
x
T

G

G

I

I

A

G

L

L

S

A

T

M

A

I

C

A

A

A

A

S

K

R

G

G

Y

Y

P

R

L

L

T

I

I

L

V

T

T

M

D

P

A

A

N

Q

V

M

Q

S

E

E

S

E

A

R

L

K

R

R

T

V

M

L

R

K

A

A

L

F

G

G

T

A

R

E

L

L

T

V

I

L

I

T

E

-

R

-

P

-

D

D

S

P

T

E

G

R

F

M

L

L

H

A

Q

A

R

R

I

E

D

E

Y

A

I

L

H

R

R

-

S

-

L

L

N

K

D

E

P

L

D

G

L

A

V

F

W

M

L

P

N

D

Q
|
Q

Y

F

S

K

N

N

P

P

A

A

N

N

V

V

W

R

A

A

H

H

T

Y

A

E

T

T

A

G

H

P

A

E

V

L

D

Y

N

E

E

A

L

L

-

E

G

G

P

R

I

I

D

D

A

A

L

F

F

V

V
x
Y

G

G

T

S

G
|
G

T
|
T

S
x
G

G

G

T
|
T

L

I

M

T

G

G

C

V

L

G

E

R

F

Y

R

L

R

K

T

E

H

R

R

I

R

P

R

H

H

V

T

K

I

V

V

I

A

A

V

V

D
x
E

-

P

S

A

E

R

G
x
S

S

N

V

V

T

L

F

S

G

G

P

K

P

M

K

G

R

Q

R

H

Y

G

I

F

P

Q

G
|
G

L
x
M

G

G

A
x
P

S

G

R

F

K

I

P

P

E

E

I

N

Y

L

S

D

E

L

A

S

E

L

S

L

F

D

D

G

K

V

V

I

L

Q

I

V

P

W

E

E

A

E

D

D

T

A

I

F

A

P

E

L

C

A

H

R

R

R

L

L

A

A

R

R

T

E

Y

E

G

G

L

L

L

F

A

L

G

G

G

M

S
|
S

T

S

G

G

T

G

V

I

L

V

A

W

A

A

I

A

R

L

K

Q

Y

V

G

A

R

R

R

E

F

L

P

G

P

P

G

G

A

K

R

R

I

V

A

A

S

C

I

I

S

S

P
|
P

D
|
D

M

G

G

G

E

W

K

K

Y

Y

L

L

P

S

T

T

active site: K40 (= K41), S70 (= S69), E200 (≠ D203), S204 (≠ G206), S263 (= S265)
binding 5-oxohexanoic acid: T69 (≠ S68), G71 (= G70), T73 (≠ L72), Q141 (= Q145), G175 (= G178), G219 (= G221), M220 (≠ L222), P222 (≠ A224)
binding pyridoxal-5'-phosphate: K40 (= K41), N72 (= N71), Y172 (≠ V175), G175 (= G178), T176 (= T179), G177 (≠ S180), T179 (= T182), G219 (= G221), S263 (= S265), P289 (= P291), D290 (= D292)

2ecqA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 3-hydroxylactate
35% identity, 86% coverage: 26:300/321 of query aligns to 25:298/302 of 2ecqA

query
sites
2ecqA

Q

E

V

V

F

W

L

V

K

K

L

L

E

E

G

G

L

L

N

N

P

P

S

G

G

G

S

S

I

I

K
|
K

A

D

K

R

A

P

A

A

A

W

S

Y

L

M

L

I

E

K

D

D

A

A

E

E

K

E

S

R

G

G

-

I

-

L

R

R

L

P

G

G

P

S

G

G

K

Q

K

V

I

I

I

V

E

E

S

P

S

T

S
|
S

G
|
G

N
|
N

L
x
T

G

G

I

I

A

G

L

L

S

A

T

M

A

I

C

A

A

A

A

S

K

R

G

G

Y

Y

P

R

L

L

T

I

I

L

V

T

T

M

D

P

A

A

N

Q

V

M

Q

S

E

E

S

E

A

R

L

K

R

R

T

V

M

L

R

K

A

A

L

F

G

G

T

A

R

E

L

L

T

V

I

L

I

T

E

-

R

-

P

-

D

D

S

P

T

E

G

R

F

M

L

L

H

A

Q

A

R

R

I

E

D

E

Y

A

I

L

H

R

R

-

S

-

L

L

N

K

D

E

P

L

D

G

L

A

V

F

W

M

L

P

N

D

Q
|
Q

Y

F

S

K

N

N

P

P

A

A

N

N

V

V

W

R

A

A

H

H

T

Y

A

E

T

T

A

G

H

P

A

E

V

L

D

Y

N

E

E

A

L

L

-

E

G

G

P

R

I

I

D

D

A

A

L

F

F

V

V
x
Y

G
|
G

T

S

G
|
G

T
|
T

S

G

G

G

T
|
T

L

I

M

T

G

G

C

V

L

G

E

R

F

Y

R

L

R

K

T

E

H

R

R

I

R

P

R

H

H

V

T

K

I

V

V

I

A

A

V

V

D
x
E

-

P

S

A

E

R

G
x
S

S

N

V

V

T

L

F

S

G

G

P

K

P

M

K

G

R

Q

R

H

Y

G

I

F

P

Q

G
|
G

L

M

G

G

A

P

S

G

R

F

K

I

P

P

E

E

I

N

Y

L

S

D

E

L

A

S

E

L

S

L

F

D

D

G

K

V

V

I

L

Q

I

V

P

W

E

E

A

E

D

D

T

A

I

F

A

P

E

L

C

A

H

R

R

R

L

L

A

A

R

R

T

E

Y

E

G

G

L

L

L

F

A

L

G

G

G

M

S
|
S

T

S

G

G

T

G

V

I

L

V

A

W

A

A

I

A

R

L

K

Q

Y

V

G

A

R

R

R

E

F

L

P

G

P

P

G

G

A

K

R

R

I

V

A

A

S

C

I

I

S

S

P
|
P

D

D

M

G

G

G

E

W

K

K

Y

Y

L

L

P

S

T

T

active site: K40 (= K41), S70 (= S69), E200 (≠ D203), S204 (≠ G206), S263 (= S265)
binding (3s)-3-hydroxybutanoic acid: K40 (= K41), G71 (= G70), T73 (≠ L72), Q141 (= Q145), G219 (= G221)
binding pyridoxal-5'-phosphate: K40 (= K41), N72 (= N71), Y172 (≠ V175), G173 (= G176), G175 (= G178), T176 (= T179), T179 (= T182), G219 (= G221), S263 (= S265), P289 (= P291)

2ecoA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 4-methylvalerate
35% identity, 86% coverage: 26:300/321 of query aligns to 25:298/302 of 2ecoA

query
sites
2ecoA

Q

E

V

V

F

W

L

V

K

K

L

L

E

E

G

G

L

L

N

N

P

P

S

G

G

G

S

S

I

I

K
|
K

A

D

K

R

A

P

A

A

A

W

S

Y

L

M

L

I

E

K

D

D

A

A

E

E

K

E

S

R

G

G

-

I

-

L

R

R

L

P

G

G

P

S

G

G

K

Q

K

V

I

I

I

V

E

E

S

P

S
x
T

S
|
S

G
|
G

N
|
N

L
x
T

G

G

I

I

A

G

L

L

S

A

T

M

A

I

C

A

A

A

A

S

K

R

G

G

Y

Y

P

R

L

L

T

I

I

L

V

T

T

M

D

P

A

A

N

Q

V

M

Q

S

E

E

S

E

A

R

L

K

R

R

T

V

M

L

R

K

A

A

L

F

G

G

T

A

R

E

L

L

T

V

I

L

I

T

E

-

R

-

P

-

D

D

S

P

T

E

G

R

F

M

L

L

H

A

Q

A

R

R

I

E

D

E

Y

A

I

L

H

R

R

-

S

-

L

L

N

K

D

E

P

L

D

G

L

A

V

F

W

M

L

P

N

D

Q
|
Q

Y

F

S

K

N

N

P

P

A

A

N

N

V

V

W

R

A

A

H

H

T

Y

A

E

T

T

A

G

H

P

A

E

V

L

D

Y

N

E

E

A

L

L

-

E

G

G

P

R

I

I

D

D

A

A

L

F

F

V

V
x
Y

G

G

T

S

G
|
G

T
|
T

S

G

G

G

T
|
T

L

I

M

T

G

G

C

V

L

G

E

R

F

Y

R

L

R

K

T

E

H

R

R

I

R

P

R

H

H

V

T

K

I

V

V

I

A

A

V

V

D
x
E

-

P

S

A

E

R

G
x
S

S

N

V

V

T

L

F

S

G

G

P

K

P

M

K

G

R

Q

R

H

Y

G

I

F

P

Q

G
|
G

L

M

G

G

A

P

S

G

R

F

K

I

P

P

E

E

I

N

Y

L

S

D

E

L

A

S

E

L

S

L

F

D

D

G

K

V

V

I

L

Q

I

V

P

W

E

E

A

E

D

D

T

A

I

F

A

P

E

L

C

A

H

R

R

R

L

L

A

A

R

R

T

E

Y

E

G

G

L

L

L

F

A

L

G

G

G

M

S
|
S

T

S

G

G

T

G

V

I

L

V

A

W

A

A

I

A

R

L

K

Q

Y

V

G

A

R

R

R

E

F

L

P

G

P

P

G

G

A

K

R

R

I

V

A

A

S

C

I

I

S

S

P
|
P

D
|
D

M

G

G

G

E

W

K

K

Y

Y

L

L

P

S

T

T

active site: K40 (= K41), S70 (= S69), E200 (≠ D203), S204 (≠ G206), S263 (= S265)
binding 4-methyl valeric acid: K40 (= K41), T69 (≠ S68), G71 (= G70), T73 (≠ L72), Q141 (= Q145), G175 (= G178), T176 (= T179), G219 (= G221)
binding pyridoxal-5'-phosphate: K40 (= K41), N72 (= N71), Y172 (≠ V175), G175 (= G178), T176 (= T179), T179 (= T182), G219 (= G221), S263 (= S265), P289 (= P291), D290 (= D292)

P16703 Cysteine synthase B; CSase B; O-acetylserine (thiol)-lyase B; OAS-TL B; O-acetylserine sulfhydrylase B; EC 2.5.1.47 from Escherichia coli (strain K12) (see paper)
31% identity, 95% coverage: 6:309/321 of query aligns to 4:299/303 of P16703

query
sites
P16703

V

L

S

E

E

Q

I

T

I

I

T

G

D

N

D

T

I

P

F

L

V

V

N

K

L

L

K

Q

D

R

F

M

L

G

P

P

Q

D

R

N

-

G

-

S

Q

E

V

V

F

W

L

L

K

K

L

L

E

E

G

G

L

N

N

N

P

P

S

A

G

G

S

S

I

V

K

K

A

D

K

R

A

A

A

L

S

S

L

M

L

I

E

V

D

E

A

A

E

E

K

K

S

R

G

G

R

E

L

I

G

K

P

P

G

G

K

D

K

V

I

L

I

I

E

E

S

A

S

T

S

S

G

G

N
|
N

L

T

G

G

I

I

A

A

L

L

S

A

T

M

A

I

C

A

A

A

A

L

K

K

G

G

Y

Y

P

R

L

M

T

K

I

L

V

L

T

M

D

P

A

D

N

N

V

M

Q

S

E

Q

S

E

A

R

L

R

R

A

T

A

M

M

R

R

A

A

L

Y

G

G

T

A

R

E

L

L

T

I

I

L

I

V

E

T

R

K

P

E

D

Q

S

G

T

M

G

E

G

G

F

A

L

R

H

D

Q

L

R

A

I

L

D

E

Y

M

I

A

H

N

R

R

S

G

L

E

N

G

D

K

D

-

P

-

D

-

L

-

V

-

W

L

L

L

N

D

Q

Q

Y

F

S

N

N

N

P

P

A

D

N

N

V

P

W

Y

A

A

H

H

T

Y

A

T

T

T

A

G

H

P

A

E

V

I

D

W

N

Q

E

Q

L

T

G

G

P

G

I

R

D

I

A

T

L

H

F

F

V

V

G

S

T

S

-

M

G

G

T

T

S

T

G

G

T

T

L

I

M

T

G

G

C

V

L

S

E

R

F

F

R

M

R

R

T

E

H

Q

R

S

R

K

R

P

H

V

T

T

I

I

V

V

A

G

V

L

D

Q

S

P

E

E

G

E

S

G

V

S

T

S

F

I

G

-

P

P

P

G

K

I

R

R

-

W

-

P

-

T

-

E

Y

Y

I

L

P

P

G

G

L

I

G

-

A

-

S

-

R

-

K

-

P

-

E

-

I

-

Y

-

S

F

E

N

A

A

E

S

S

L

F

V

D

D

K

E

V

V

L

L

-

D

I

I

P

H

E

Q

A

R

D

D

T

A

I

E

A

N

E

T

C

M

H

R

R

E

L

L

A

A

R

V

T

R

Y

E

G

G

L

I

L

F

A

C

G

G

G

V

S
|
S

T

S

G

G

T

G

V

A

L

V

A

A

A

G

I

A

R

L

K

R

Y

V

G

A

R

K

R

A

F

N

P

P

P

D

G

A

A

V

R

V

I

V

A

A

S

I

I

I

S

C

P

-

D

D

M

R

G

G

E

D

K

R

Y

Y

L

L

P

S

T

T

-

G

V

V

Y

F

S

G

H

E

E

E

W

H

L

F

S

S

R

Q

N71 (= N71) binding pyridoxal 5'-phosphate
S255 (= S265) binding pyridoxal 5'-phosphate

5xoqA Crystal structure of o-acetylserine sulfhydrylase with bound transcription factor peptide inhibitor from planctomyces limnophilus
31% identity, 93% coverage: 2:300/321 of query aligns to 4:303/310 of 5xoqA

query
sites
5xoqA

L

I

Y

F

N

K

D

D

V

N

S

S

E

E

I

S

I

I

T

G

D

R

D

T

I

P

F

L

V

V

N

Q

L

I

K

N

D

R

F

L

L

T

P

A

-

G

-

L

Q

S

R

S

Q

R

V

V

F

L

L

A

K

K

L

I

E

E

G

G

L

R

N

N

P

P

S

A

G

Y

S

S

I

V

K

K

A

C

K

R

A

I

A

G

A

A

S

A

L

M

L

I

E

W

D

D

A

A

E

E

K

Q

S

S

G

G

R

K

L

L

G

K

P

P

G

G

K

M

K

H

I

V

E

E

S

P

S
x
T

S
|
S

G
|
G

N

N

L
x
T

G

G

I

I

A

A

L

L

S

A

T

F

A

V

C

C

A

A

K

R

G

G

Y

Y

P

K

L

L

T

T

I

L

V

T

T

M

D

P

A

E

N

T

V

M

Q

S

E

I

S

E

A

R

L

R

R

M

T

M

M

L

R

K

A

S

L

F

G

G

T

A

R

D

L

L

T

V

I

L

I

T

E

P

R

G

P

A

D

D

S

G

T
x
M

G

K

G

G

F

A

L

I

H

S

Q

K

R

A

I

-

D

-

Y

-

I

-

H

-

R

E

S

E

L

L

N

A

D

A

D

Q

P

P

D

G

L

W

V

F

W

I

L

P

N

Q

Q
|
Q

Y

F

S

K

N

N

P

P

A

A

N

N

V

P

W

A

A

I

H

H

T

V

A

K

T

T

A

G

H

P

A

E

V

I

D

W

N

N

E

D

L

T

-

E

G

G

P

Q

I

V

D

D

A

V

L

F

F

V

V

A

G

G

T

V

G

G

T

T

S

G

G

G

T

T

L

I

M

T

G

G

C

V

L

A

E

R

F

F

R

L

R

K

T

-

H

H

R

E

R

K

H

H

T

P

-

V

-

H

I

V

V

V

A

A

V

V

D

E

S

P

E

A

G

A

S

S

-

P

V

V

T

L

F

A

G

G

P

P

P

A

K

G

R
|
R

R
x
H

Y

K

I

I

P
x
Q

G
|
G

L

I

G

G

A
|
A

S

G

R

F

K

V

P

P

E

D

I

T

Y

F

S

D

E

R

A

S

E

V

S

V

F

D

D

E

K

I

V

L

L

S

I

V

P

T

E

D

A

D

D

E

T

A

I

I

A

E

E

T

C

A

H

R

R

K

L

L

A

A

R

M

T

E

Y

E

G

G

L

I

L

S

A

C

G

G

G

I

S

S

T

C

G

G

T

A

V

A

L

M

A

A

-

G

A

A

I

L

R

K

K

V

Y

A

G

A

R

R

R

P

F

E

P

F

P

A

G

G

A

K

R

T

I

I

A

V

S

T

I

V

S

L

P

P

D

D

M

A

G

G

E

E

K

R

Y

Y

L

L

P

S

T

T

binding : T72 (≠ S68), S73 (= S69), G74 (= G70), T76 (≠ L72), M123 (≠ T119), Q144 (= Q145), R218 (= R216), H219 (≠ R217), Q222 (≠ P220), G223 (= G221), A226 (= A224)

3bm5A Crystal structure of o-acetyl-serine sulfhydrylase from entamoeba histolytica in complex with cysteine (see paper)
32% identity, 86% coverage: 26:300/321 of query aligns to 42:315/338 of 3bm5A

query
sites
3bm5A

Q

R

V

I

F

L

L

V

K

K

L

L

E

E

G

Y

L

F

N

N

P

P

S

M

G

S

S

S

I

V

K
|
K

A

D

K

R

A

V

A

G

A

F

S

N

L

I

L

V

E

Y

D

Q

A

A

E

I

K

K

S

D

G

G

R

R

L

L

G

K

P

P

G

G

K

M

K

E

I

I

E

E

S

S

S

T

S

S

G

G

N
|
N

L

T

G

G

I

I

A

A

L

L

S

C

T

Q

A

A

C

G

A

A

A

V

K

F

G

G

Y

Y

P

R

L

V

T

N

I

I

V

A

T

M

D

P

A

S

N

T

V

M

Q

S

E

V

S

E

A

R

L

Q

R

M

T

I

M

M

R

K

A

A

L

F

G

G

T

A

R

E

L

L

T

I

I

L

I

T

E

E

R

G

P

K

D

K

S

G

T

M

G

P

G

G

F

-

L

-

H

-

Q

-

R

A

I

I

D

E

Y

E

I

V

H

N

R

K

S

M

L

I

N

K

D

E

D

N

P

P

D

G

L

K

V

Y

W

F

L

V

-

A

N

N

Q

Q

Y

F

S

G

N

N

P

P

A

D

N

N

V

T

W

A

A

A

H

H

T

H

A

Y

T

T

T

A

A

N

H

E

A

I

V

W

D

E

N

D

E

T

L

D

G

G

P

E

I

V

D

D

A

I

L

V

F

V

V

S

G

A

T

V

G
|
G

T
|
T

S
|
S

G

G

T
|
T

L

V

M

I

G

G

C

V

L

A

E

E

F

K

R

L

R

K

T

E

H

K

R

K

R

G

H

I

T

K

I

I

V

I

A

A

V

V

D

E

S

P

E

E

G

E

S

S

V

A

T

V

F

L

G

E

G

G

P

K

P

A

K

K

R

G

R

P

Y

H

-

G

I

I

P

Q

G
|
G

L

I

G

G

A

A

S

G

R

F

K

I

P

P

E

D

I

I

Y

Y

S

K

E

K

A

-

E

E

S

F

F

V

D

D

K

E

V

I

L

I

-

P

I

I

P

K

E

T

A

Q

D

D

T

A

I

W

A

K

E

M

C

A

H

R

R

A

L

V

A

V

R

K

T

Y

Y

D

G

G

L

I

L

M

A

C

G

G

G

M

S
|
S

T

S

G

G

-

A

T

A

V

I

L

L

A

A

A

G

I

L

R

K

K

E

Y

A

G

E

R

K

R

P

F

E

P

N

P

E

G

G

A

K

R

T

I

I

A

V

S

I

I

I

S

V

P
|
P

D

S

M

C

G

G

E

E

K

R

Y

Y

L

L

P

S

T

T

active site: K57 (= K41), S279 (= S265)
binding pyridoxal-5'-phosphate: K57 (= K41), N87 (= N71), G191 (= G178), T192 (= T179), S193 (= S180), T195 (= T182), G235 (= G221), S279 (= S265), P306 (= P291)

4jbnA Crystal structure of o-acetyl serine sulfhydrylase from entamoeba histolytica in complex with serine acetyl transferase derived tetrapeptide, spsi (see paper)
32% identity, 86% coverage: 26:300/321 of query aligns to 42:315/336 of 4jbnA

query
sites
4jbnA

Q

R

V

I

F

L

L

V

K

K

L

L

E

E

G

Y

L

F

N

N

P

P

S

M

G

S

S

S

I

V

K
|
K

A

D

K

R

A

V

A

G

A

F

S

N

L

I

L

V

E

Y

D

Q

A

A

E

I

K

K

S

D

G

G

R

R

L

L

G

K

P

P

G

G

K

M

K

E

I

I

E

E

S

S

S
x
T

S
|
S

G
|
G

N

N

L
x
T

G

G

I

I

A

A

L

L

S

C

T

Q

A

A

C

G

A

A

A

V

K

F

G

G

Y

Y

P

R

L

V

T

N

I

I

V

A

T

M

D

P

A

S

N

T

V

M

Q

S

E

V

S

E

A

R

L

Q

R

M

T

I

M

M

R

K

A

A

L

F

G

G

T

A

R

E

L

L

T

I

I

L

I

T

E

E

R

G

P

K

D

K

S

G

T

M

G

P

G

G

F

-

L

-

H

-

Q

-

R

A

I

I

D

E

Y

E

I

V

H

N

R

K

S

M

L

I

N

K

D

E

D

N

P

P

D

G

L

K

V

Y

W

F

L

V

-

A

N

N

Q
|
Q

Y

F

S

G

N

N

P

P

A

D

N

N

V

T

W

A

A

A

H

H

T

H

A

Y

T

T

T

A

A

N

H

E

A

I

V

W

D

E

N

D

E

T

L

D

G

G

P

E

I

V

D

D

A

I

L

V

F

V

V

S

G

A

T

V

G
|
G

T

T

S

S

G

G

T

T

L

V

M

I

G

G

C

V

L

A

E

E

F

K

R

L

R

K

T

E

H

K

R

K

R

G

H

I

T

K

I

I

V

I

A

A

V

V

D

E

S

P

E

E

G

E

S

S

V

A

T

V

F

L

G

E

G

G

P

K

P

A

K

K

R

G

R

P

Y

H

-

G

I

I

P

Q

G

G

L

I

G

G

A

A

S

G

R

F

K

I

P

P

E

D

I

I

Y

Y

S

K

E

K

A

-

E

E

S

F

F

V

D

D

K

E

V

I

L

I

-

P

I

I

P

K

E

T

A

Q

D

D

T

A

I

W

A

K

E

M

C

A

H

R

R

A

L

V

A

V

R

K

T

Y

Y

D

G

G

L

I

L

M

A

C

G

G

G

M

S
|
S

T

S

G

G

-

A

T

A

V

I

L

L

A

A

A

G

I

L

R

K

K

E

Y

A

G

E

R

K

R

P

F

E

P

N

P

E

G

G

A

K

R

T

I

I

A

V

S

I

I

I

S

V

P

P

D

S

M

C

G

G

E

E

K

R

Y

Y

L

L

P

S

T

T

active site: K57 (= K41), S279 (= S265)
binding : K57 (= K41), T84 (≠ S68), S85 (= S69), G86 (= G70), T88 (≠ L72), Q158 (= Q145), G191 (= G178)

4il5A Crystal structure of o-acetyl serine sulfhydrylase from entamoeba histolytica in complex with isoleucine (see paper)
32% identity, 86% coverage: 26:300/321 of query aligns to 42:315/336 of 4il5A

query
sites
4il5A

Q

R

V

I

F

L

L

V

K

K

L

L

E

E

G

Y

L

F

N

N

P

P

S

M

G

S

S

S

I

V

K
|
K

A

D

K

R

A

V

A

G

A

F

S

N

L

I

L

V

E

Y

D

Q

A

A

E

I

K

K

S

D

G

G

R

R

L

L

G

K

P

P

G

G

K

M

K

E

I

I

E

E

S

S

S

T

S

S

G
|
G

N

N

L
x
T

G

G

I

I

A

A

L

L

S

C

T

Q

A

A

C

G

A

A

A

V

K

F

G

G

Y

Y

P

R

L

V

T

N

I

I

V

A

T

M

D

P

A

S

N

T

V

M

Q

S

E

V

S

E

A

R

L

Q

R

M

T

I

M

M

R

K

A

A

L

F

G

G

T

A

R

E

L

L

T

I

I

L

I

T

E

E

R

G

P

K

D

K

S

G

T

M

G

P

G

G

F

-

L

-

H

-

Q

-

R

A

I

I

D

E

Y

E

I

V

H

N

R

K

S

M

L

I

N

K

D

E

D

N

P

P

D

G

L

K

V

Y

W

F

L

V

-

A

N

N

Q
|
Q

Y
x
F

S

G

N

N

P

P

A

D

N

N

V

T

W

A

A

A

H

H

T

H

A

Y

T

T

T

A

A

N

H

E

A

I

V

W

D

E

N

D

E

T

L

D

G

G

P

E

I

V

D

D

A

I

L

V

F

V

V

S

G

A

T

V

G
|
G

T

T

S

S

G

G

T

T

L

V

M

I

G

G

C

V

L

A

E

E

F

K

R

L

R

K

T

E

H

K

R

K

R

G

H

I

T

K

I

I

V

I

A

A

V

V

D

E

S

P

E

E

G

E

S

S

V

A

T

V

F

L

G

E

G

G

P

K

P

A

K

K

R

G

R

P

Y

H

-

G

I

I

P

Q

G
|
G

L

I

G

G

A

A

S

G

R

F

K

I

P

P

E

D

I

I

Y

Y

S

K

E

K

A

-

E

E

S

F

F

V

D

D

K

E

V

I

L

I

-

P

I

I

P

K

E

T

A

Q

D

D

T

A

I

W

A

K

E

M

C

A

H

R

R

A

L

V

A

V

R

K

T

Y

Y

D

G

G

L

I

L

M

A

C

G

G

G

M

S
|
S

T

S

G

G

-

A

T

A

V

I

L

L

A

A

A

G

I

L

R

K

K

E

Y

A

G

E

R

K

R

P

F

E

P

N

P

E

G

G

A

K

R

T

I

I

A

V

S

I

I

I

S

V

P

P

D

S

M

C

G

G

E

E

K

R

Y

Y

L

L

P

S

T

T

active site: K57 (= K41), S279 (= S265)
binding isoleucine: K57 (= K41), G86 (= G70), T88 (≠ L72), Q158 (= Q145), F159 (≠ Y146), G191 (= G178), G235 (= G221)

4jblB Crystal structure of o-acetyl serine sulfhydrylase from entamoeba histolytica in complex with methionine (see paper)
32% identity, 86% coverage: 26:300/321 of query aligns to 42:315/335 of 4jblB

query
sites
4jblB

Q

R

V

I

F

L

L

V

K

K

L

L

E

E

G

Y

L

F

N

N

P

P

S

M

G

S

S

S

I

V

K
|
K

A

D

K

R

A

V

A

G

A

F

S

N

L

I

L

V

E

Y

D

Q

A

A

E

I

K

K

S

D

G

G

R

R

L

L

G

K

P

P

G

G

K

M

K

E

I

I

E

E

S

S

S
x
T

S
|
S

G

G

N
|
N

L
x
T

G

G

I

I

A

A

L

L

S

C

T

Q

A

A

C

G

A

A

A

V

K

F

G

G

Y

Y

P

R

L

V

T

N

I

I

V

A

T

M

D

P

A

S

N

T

V

M

Q

S

E

V

S

E

A

R

L

Q

R

M

T

I

M

M

R

K

A

A

L

F

G

G

T

A

R

E

L

L

T

I

I

L

I

T

E

E

R

G

P

K

D

K

S

G

T
x
M

G

P

G

G

F

-

L

-

H

-

Q

-

R

A

I

I

D

E

Y

E

I

V

H

N

R

K

S

M

L

I

N

K

D

E

D

N

P

P

D

G

L

K

V

Y

W

F

L

V

-

A

N

N

Q
|
Q

Y
x
F

S

G

N

N

P

P

A

D

N

N

V

T

W

A

A

A

H

H

T

H

A

Y

T

T

T

A

A

N

H

E

A

I

V

W

D

E

N

D

E

T

L

D

G

G

P

E

I

V

D

D

A

I

L

V

F

V

V

S

G

A

T

V

G
|
G

T

T

S

S

G

G

T

T

L

V

M

I

G

G

C

V

L

A

E

E

F

K

R

L

R

K

T

E

H

K

R

K

R

G

H

I

T

K

I

I

V

I

A

A

V

V

D

E

S

P

E

E

G

E

S

S

V

A

T

V

F

L

G

E

G

G

P

K

P

A

K

K

R

G

R

P

Y

H

-

G

I

I

P

Q

G
|
G

L
x
I

G

G

A
|
A

S

G

R

F

K

I

P

P

E

D

I

I

Y

Y

S

K

E

K

A

-

E

E

S

F

F

V

D

D

K

E

V

I

L

I

-

P

I

I

P

K

E

T

A

Q

D

D

T

A

I

W

A

K

E

M

C

A

H

R

R

A

L

V

A

V

R

K

T

Y

Y

D

G

G

L

I

L

M

A

C

G

G

G

M

S
|
S

T

S

G

G

-

A

T

A

V

I

L

L

A

A

A

G

I

L

R

K

K

E

Y

A

G

E

R

K

R

P

F

E

P

N

P

E

G

G

A

K

R

T

I

I

A

V

S

I

I

I

S

V

P

P

D

S

M

C

G

G

E

E

K

R

Y

Y

L

L

P

S

T

T

active site: K57 (= K41), S279 (= S265)
binding methionine: K57 (= K41), T84 (≠ S68), S85 (= S69), N87 (= N71), T88 (≠ L72), M135 (≠ T119), Q158 (= Q145), F159 (≠ Y146), G191 (= G178), G235 (= G221), I236 (≠ L222), A238 (= A224)

3bm5B Crystal structure of o-acetyl-serine sulfhydrylase from entamoeba histolytica in complex with cysteine (see paper)
32% identity, 86% coverage: 26:300/321 of query aligns to 42:315/335 of 3bm5B

query
sites
3bm5B

Q

R

V

I

F

L

L

V

K

K

L

L

E

E

G

Y

L

F

N

N

P

P

S

M

G

S

S

S

I

V

K
|
K

A

D

K

R

A

V

A

G

A

F

S

N

L

I

L

V

E

Y

D

Q

A

A

E

I

K

K

S

D

G

G

R

R

L

L

G

K

P

P

G

G

K

M

K

E

I

I

E

E

S

S

S
x
T

S
|
S

G

G

N
|
N

L
x
T

G

G

I

I

A

A

L

L

S

C

T

Q

A

A

C

G

A

A

A

V

K

F

G

G

Y

Y

P

R

L

V

T

N

I

I

V

A

T

M

D

P

A

S

N

T

V

M

Q

S

E

V

S

E

A

R

L

Q

R

M

T

I

M

M

R

K

A

A

L

F

G

G

T

A

R

E

L

L

T

I

I

L

I

T

E

E

R

G

P

K

D

K

S

G

T

M

G

P

G

G

F

-

L

-

H

-

Q

-

R

A

I

I

D

E

Y

E

I

V

H

N

R

K

S

M

L

I

N

K

D

E

D

N

P

P

D

G

L

K

V

Y

W

F

L

V

-

A

N

N

Q

Q

Y

F

S

G

N

N

P

P

A

D

N

N

V

T

W

A

A

A

H

H

T

H

A

Y

T

T

T

A

A

N

H

E

A

I

V

W

D

E

N

D

E

T

L

D

G

G

P

E

I

V

D

D

A

I

L

V

F

V

V

S

G

A

T

V

G
|
G

T
|
T

S
|
S

G

G

T
|
T

L

V

M

I

G

G

C

V

L

A

E

E

F

K

R

L

R

K

T

E

H

K

R

K

R

G

H

I

T

K

I

I

V

I

A

A

V

V

D

E

S

P

E

E

G

E

S

S

V

A

T

V

F

L

G

E

G

G

P

K

P

A

K

K

R

G

R

P

Y

H

-

G

I

I

P

Q

G
|
G

L

I

G

G

A

A

S

G

R

F

K

I

P

P

E

D

I

I

Y

Y

S

K

E

K

A

-

E

E

S

F

F

V

D

D

K

E

V

I

L

I

-

P

I

I

P

K

E

T

A

Q

D

D

T

A

I

W

A

K

E

M

C

A

H

R

R

A

L

V

A

V

R

K

T

Y

Y

D

G

G

L

I

L

M

A

C

G

G

G

M

S
|
S

T

S

G

G

-

A

T

A

V

I

L

L

A

A

A

G

I

L

R

K

K

E

Y

A

G

E

R

K

R

P

F

E

P

N

P

E

G

G

A

K

R

T

I

I

A

V

S

I

I

I

S

V

P
|
P

D

S

M

C

G

G

E

E

K

R

Y

Y

L

L

P

S

T

T

active site: K57 (= K41), S279 (= S265)
binding cysteine: K57 (= K41), T84 (≠ S68), S85 (= S69), T88 (≠ L72), G235 (= G221)
binding pyridoxal-5'-phosphate: K57 (= K41), N87 (= N71), G191 (= G178), T192 (= T179), S193 (= S180), T195 (= T182), G235 (= G221), S279 (= S265), P306 (= P291)

6kr5B Crystal structure of o-acetyl serine sulfhydrylase isoform 3 from entamoeba histolytica (see paper)
31% identity, 86% coverage: 26:300/321 of query aligns to 41:314/336 of 6kr5B

query
sites
6kr5B

Q

K

V

I

F

L

L

V

K

K

L

L

E

E

G

C

L

F

N

N

P

P

S

M

G

S

S

S

I

V

K
|
K

A

D

K

R

A

V

A

G

A

F

S

N

L

I

E

Y

D

Q

A

A

E

I

K

K

S

D

G

G

R

R

L

L

G

K

P

P

G

G

K

M

K

E

I

I

E

E

S

A

S

T

S

S

G

G

N
|
N

L

T

G

G

I

I

A

G

L

L

S

C

T

Q

A

A

C

G

A

A

A

V

K

F

G

G

Y

Y

P

P

L

V

T

N

I

I

V

V

T

M

D

P

A

S

N

T

V

M

Q

S

E

V

S

E

A

R

L

Q

R

M

T

I

M

M

R

K

A

A

L

F

G

G

T

A

R

N

L

L

T

V

I

L

I

S

E

D

R

G

P

T

D

K

S

G

T

M

G

P

G

G

F

A

L

I

H

A

Q

K

R

Y

I

E

D

E

Y

L

I

I

H

K

R

Q

S

H

L

P

N

N

D

-

P

-

D

K

L

Y

V

F

W

P

L

A

N

N

Q

Q

Y

F

S

G

N

N

P

P

A

D

N

N

V

T

W

A

A

A

H

H

T

V

A

Y

T

T

T

A

A

N

H

E

A

I

V

W

D

E

N

D

E

T

L

N

G

G

P

E

I

V

D

D

A

I

L

I

F

V

V

S

G

A

T
x
V

G
|
G

T
|
T

S
x
A

G

G

T
|
T

L

V

M

I

G

G

C

V

L

G

E

E

F

N

R

L

R

K

T

K

H

K

R

K

R

G

H

V

T

K

I

V

V

V

A

A

V

V

D

E

S

P

E

A

G

E

S

S

V

A

T

V

F

L

G

S

G

G

P

K

P

P

K

K

R

G

R

P

Y

H

-

G

I

I

P

Q

G
|
G

L

I

G

G

A

A

S

G

R

F

K

V

P

T

E

D

I

I

Y

Y

S

K

E

K

A

-

E

E

S

V

F

V

D

D

K

E

V

I

L

T

-

P

I

I

P

K

E

T

A

Q

D

D

T

A

I

W

A

K

E

M

C

A

H

R

R

A

L

V

A

V

R

K

T

Y

Y

D

G

G

L

I

L

M

A

C

G

G

G

M

S
|
S

T

S

G

G

-

A

T

A

V

I

L

L

A

A

A

G

I

L

R

K

K

E

Y

A

G

G

R

K

R

V

F

E

P

N

P

E

G

G

A

K

R

T

I

I

A

V

S

I

I

I

S

L

P
|
P

D
|
D

M

C

G

G

E

E

K

R

Y

Y

L

L

P

S

T

T

binding pyridoxal-5'-phosphate: K56 (= K41), N86 (= N71), V189 (≠ T177), G190 (= G178), T191 (= T179), A192 (≠ S180), T194 (= T182), G234 (= G221), S278 (= S265), P305 (= P291), D306 (= D292)

4lmbA Crystal structure analysis of o-acetylserine sulfhydrylase cysk2 complexed with cystine from microcystis aeruginosa 7806 (see paper)
33% identity, 93% coverage: 1:300/321 of query aligns to 2:305/310 of 4lmbA

query
sites
4lmbA

M

L

L

I

Y

A

N

R

D

D

V

I

S

T

E

Q

I

L

I

V

T

G

D

R

D

T

I

P

F

L

V

V

N

Q

L

L

K

-

D

N

F

R

L

I

P

P

-

V

-

A

-

E

-

G

-

V

Q

K

R

A

Q

R

V

I

F

V

L

V

K

K

L

L

E

E

G

S

L

M

N

N

P

P

S

A

G

A

S

S

I

V

K
|
K

A

D

K

R

A

I

A

G

A

V

S

S

L

M

L

V

E

E

D

D

A

A

E

E

K

A

S

A

G

G

R

L

L

I

G

H

P

P

G

D

K

K

K

T

I

I

-

L

I

V

E

E

S

P

S
x
T

S
|
S

G

G

N
|
N

L
x
T

G

G

I

I

A

A

L

L

S

A

T

M

A

V

C

A

A

A

K

K

G

G

Y

Y

P

R

L

L

T

V

I

L

V

T

T

M

D

P

A

E

N

T

V
x
M

Q

S

E

L

S

E

A

R

L

R

R

A

T

M

M

L

R

K

A

A

L

Y

G

G

T

A

R

Q

L

L

T

E

I

L

I

T

E

-

R

-

P

P

D

G

S

S

T

Q

G

G

G

-

F

-

L
x
M

H

E

Q

G

R

A

I

I

D

T

Y

R

I

A

H

E

R

E

S

I

L

V

N

E

D

N

D

T

P

P

D

N

L

A

V

Y

W

S

L

L

N

Q

Q
|
Q

Y
x
F

S

R

N

N

P

P

A

A

N

N

V

P

W

K

A

I

H

H

T

R

A

E

T

T

A

A

H

E

A

E

V

I

-

W

-

A

D

D

N

T

E

D

L

-

G

G

P

L

I

V

D

D

A

I

L

V

F

I

V

G

G

G

T
x
V

G
|
G

T
|
T

S
x
G

G

G

T
|
T

L

I

M

T

G

G

C

I

L

A

E

E

F

T

R

I

R

K

T

P

H

R

R

R

R

P

R

Q

H

F

T

Q

I

A

V

I

A

A

V

V

D

E

S

P

E

S

G

N

S

S

-

P

V

V

T

L

F

S

G

G

P

Q

P

P

K

G

R

P

R

H

Y

K

I

I

P
x
Q

G
|
G

L
x
I

G

G

A
|
A

S

G

R

F

K

I

P

P

E

A

I

I

Y

F

S

-

E

R

A

P

E

E

S

L

F

I

D

D

K

E

V

V

L

I

I

I

-

V

P

D

E

D

A

T

D

E

T

A

I

F

A

A

E

Y

C

A

H

R

R

R

L

L

A

A

R

R

T

Q

Y

E

G

G

L

L

A

S

G

G

G

I

S
|
S

T

A

G

G

T

A

V

A

L

L

A

W

A

A

I

A

R

I

K

Q

Y

V

G

G

R

K

R

R

F

P

P

E

P

N

G

E

A

D

R

K

-

L

I

I

A

V

S

M

I

I

S

Q

P
|
P

D

S

M

F

G

G

E

E

K

R

Y

Y

L

L

P

S

T

T

active site: K46 (= K41), S269 (= S265)
binding cysteine: K46 (= K41), T74 (≠ S68), S75 (= S69), N77 (= N71), T78 (≠ L72), M101 (≠ V95), M125 (≠ L123), M125 (≠ L123), Q147 (= Q145), F148 (≠ Y146), Q224 (≠ P220), G225 (= G221), G225 (= G221), I226 (≠ L222), A228 (= A224)
binding pyridoxal-5'-phosphate: K46 (= K41), N77 (= N71), V180 (≠ T177), G181 (= G178), T182 (= T179), G183 (≠ S180), T185 (= T182), G225 (= G221), S269 (= S265), P296 (= P291)

Query Sequence

>WP_025273295.1 NCBI__GCF_000527155.1:WP_025273295.1
MLYNDVSEIITDDIFVNLKDFLPQRQVFLKLEGLNPSGSIKAKAAASLLEDAEKSGRLGP
GKKIIESSSGNLGIALSTACAAKGYPLTIVTDANVQESALRTMRALGTRLTIIERPDSTG
GFLHQRIDYIHRSLNDDPDLVWLNQYSNPANVWAHTATTAHAVDNELGPIDALFVGTGTS
GTLMGCLEFRRTHRRRHTIVAVDSEGSVTFGGPPKRRYIPGLGASRKPEIYSEAESFDKV
LIPEADTIAECHRLARTYGLLAGGSTGTVLAAIRKYGRRFPPGARIASISPDMGEKYLPT
VYSHEWLSRHPDLIDKETTSV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory