SitesBLAST
Comparing WP_025274241.1 NCBI__GCF_000527155.1:WP_025274241.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5nccA Structure of fatty acid photodecarboxylase in complex with fad and palmitic acid (see paper)
34% identity, 97% coverage: 1:539/553 of query aligns to 19:578/578 of 5nccA
- active site: R347 (≠ Q327), L420 (≠ I384), I421 (≠ G385), S507 (≠ A469), A509 (≠ H471), G552 (= G513), Q553 (≠ N514)
- binding flavin-adenine dinucleotide: G30 (= G12), G32 (= G14), T33 (≠ S15), A34 (= A16), L53 (≠ I35), E54 (= E36), A55 (= A37), F74 (≠ I58), W80 (= W65), A98 (≠ G83), G100 (= G85), G105 (= G90), S106 (= S91), N110 (= N95), A111 (≠ G96), T112 (≠ M97), L113 (≠ I98), V238 (= V223), A278 (≠ G256), H282 (≠ N260), L286 (= L264), N508 (≠ Y470), Q553 (≠ N514), T554 (≠ I515), G555 (≠ Y516), V558 (= V519)
8b7sA Crystal structure of the chloramphenicol-inactivating oxidoreductase from novosphingobium sp (see paper)
34% identity, 97% coverage: 4:537/553 of query aligns to 3:457/458 of 8b7sA
- binding flavin-adenine dinucleotide: G11 (= G12), G13 (= G14), S14 (= S15), A15 (= A16), E35 (= E36), A36 (= A37), W47 (= W65), P65 (≠ G83), G67 (= G85), V180 (= V223), A214 (≠ G256), G215 (= G257), A218 (≠ N260), T270 (= T338), Y391 (= Y470), A424 (= A504), I435 (= I515), N436 (≠ Y516)
6yrvAAA structure of fap after illumination at 100k (see paper)
34% identity, 97% coverage: 1:539/553 of query aligns to 3:569/573 of 6yrvAAA
- binding carbon dioxide: R375 (≠ H361), N499 (≠ Y470)
- binding flavin-adenine dinucleotide: G14 (= G12), G16 (= G14), T17 (≠ S15), A18 (= A16), L37 (≠ I35), E38 (= E36), A39 (= A37), F58 (≠ I58), W64 (= W65), A82 (≠ G83), G89 (= G90), S90 (= S91), N94 (= N95), A95 (≠ G96), T96 (≠ M97), L97 (≠ I98), M191 (≠ N192), V222 (= V223), C264 (≠ S255), A265 (≠ G256), G266 (= G257), H269 (≠ N260), N499 (≠ Y470), A534 (= A504), Q544 (≠ N514), T545 (≠ I515), G546 (≠ Y516)
- binding heptadecane: V377 (≠ L363), G379 (vs. gap), M380 (≠ I365), G386 (= G371), T389 (≠ V374), Y390 (≠ D375), F393 (vs. gap), T408 (≠ Q381), Q410 (≠ H383)
A0A248QE08 Fatty acid photodecarboxylase, chloroplastic; CvFAP; EC 4.1.1.106 from Chlorella variabilis (Green alga) (see paper)
33% identity, 97% coverage: 1:536/553 of query aligns to 79:642/654 of A0A248QE08
- TA 93:94 (≠ SA 15:16) binding FAD
- E114 (= E36) binding FAD
- L162 (≠ V87) binding FAD
- S166 (= S91) binding FAD
- NATL 170:173 (≠ NGMI 95:98) binding FAD
- V298 (= V223) binding FAD
- C432 (≠ F341) binding hexadecanoate
- R451 (≠ H361) binding hexadecanoate
- Y466 (≠ D375) binding hexadecanoate
- Q486 (≠ H383) binding hexadecanoate
- G622 (≠ Y516) binding FAD
3ljpA Crystal structure of choline oxidase v464a mutant (see paper)
35% identity, 96% coverage: 1:530/553 of query aligns to 9:526/530 of 3ljpA
- active site: I333 (≠ A343), P377 (≠ I384), N378 (≠ G385), A464 (= A469), H466 (= H471), V509 (≠ G513), N510 (= N514)
- binding dihydroflavine-adenine dinucleotide: G22 (= G14), S23 (= S15), E44 (= E36), A45 (= A37), W71 (= W65), R89 (= R84), A90 (≠ G85), G95 (= G90), C96 (≠ S91), H99 (≠ I94), N100 (= N95), S101 (≠ G96), I103 (= I98), A232 (≠ V223), T269 (≠ G256), D273 (≠ N260), Y465 (= Y470), H466 (= H471), D499 (= D503), A500 (= A504), N510 (= N514), P511 (≠ I515), N512 (≠ Y516), V515 (= V519)
2jbvA Crystal structure of choline oxidase reveals insights into the catalytic mechanism (see paper)
35% identity, 96% coverage: 1:530/553 of query aligns to 9:526/527 of 2jbvA
- active site: I333 (≠ A343), P377 (≠ I384), N378 (≠ G385), V464 (≠ A469), H466 (= H471), V509 (≠ G513), N510 (= N514)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-5-[(4aS,10aR)-7,8-dimethyl-2,4-dioxo-1,3,4,4a,5,10a-hexahydrobenzo[g]pteridin-10(2H)-yl]-2,3,4-trihydroxypentyl dihydrogen diphosphate: G22 (= G14), S23 (= S15), E44 (= E36), A45 (= A37), W71 (= W65), A90 (≠ G85), G95 (= G90), C96 (≠ S91), H99 (≠ I94), N100 (= N95), S101 (≠ G96), I103 (= I98), R231 (= R222), A232 (≠ V223), T269 (≠ G256), G270 (= G257), D273 (≠ N260), V464 (≠ A469), Y465 (= Y470), H466 (= H471), D499 (= D503), A500 (= A504), N510 (= N514), P511 (≠ I515), N512 (≠ Y516), V515 (= V519)
4mjwA Crystal structure of choline oxidase in complex with the reaction product glycine betaine (see paper)
35% identity, 96% coverage: 1:530/553 of query aligns to 9:526/532 of 4mjwA
- active site: I333 (≠ A343), P377 (≠ I384), N378 (≠ G385), V464 (≠ A469), H466 (= H471), V509 (≠ G513), N510 (= N514)
- binding flavin-adenine dinucleotide: G20 (= G12), G22 (= G14), S23 (= S15), E44 (= E36), A45 (= A37), W71 (= W65), R89 (= R84), A90 (≠ G85), G95 (= G90), C96 (≠ S91), H99 (≠ I94), N100 (= N95), S101 (≠ G96), I103 (= I98), R231 (= R222), A232 (≠ V223), T269 (≠ G256), G270 (= G257), D273 (≠ N260), Y465 (= Y470), H466 (= H471), A500 (= A504), N510 (= N514), P511 (≠ I515), N512 (≠ Y516), V515 (= V519)
3t37A Crystal structure of pyridoxine 4-oxidase from mesorbium loti
32% identity, 94% coverage: 7:527/553 of query aligns to 3:501/509 of 3t37A
- active site: F360 (≠ I384), G361 (= G385), H444 (≠ A469), H446 (= H471), G487 (= G513), P488 (≠ N514)
- binding flavin-adenine dinucleotide: G8 (= G12), G10 (= G14), S11 (= S15), A12 (= A16), E32 (= E36), A33 (= A37), W58 (= W65), R77 (= R84), G78 (= G85), R79 (≠ K86), G83 (= G90), S84 (= S91), H88 (≠ N95), A89 (≠ G96), G91 (≠ I98), R217 (= R222), V218 (= V223), A251 (≠ G256), E255 (≠ N260), H445 (≠ Y470), A478 (= A504), P488 (≠ N514), I489 (= I515), H490 (≠ Y516)
4ha6A Crystal structure of pyridoxine 4-oxidase - pyridoxamine complex (see paper)
32% identity, 94% coverage: 7:527/553 of query aligns to 3:501/508 of 4ha6A
- active site: F360 (≠ I384), G361 (= G385), H444 (≠ A469), H446 (= H471), G487 (= G513), P488 (≠ N514)
- binding flavin-adenine dinucleotide: G8 (= G12), G10 (= G14), S11 (= S15), A12 (= A16), E32 (= E36), A33 (= A37), W58 (= W65), R77 (= R84), G78 (= G85), G83 (= G90), S84 (= S91), L87 (≠ I94), H88 (≠ N95), A89 (≠ G96), M90 (= M97), G91 (≠ I98), V218 (= V223), A251 (≠ G256), G252 (= G257), E255 (≠ N260), H445 (≠ Y470), A478 (= A504), P488 (≠ N514), I489 (= I515), H490 (≠ Y516)
- binding 4-(aminomethyl)-5-(hydroxymethyl)-2-methylpyridin-3-ol: A89 (≠ G96), S314 (≠ F341), H444 (≠ A469), H446 (= H471)
5oc1A Crystal structure of aryl-alcohol oxidase from pleurotus eryngii in complex with p-anisic acid (see paper)
31% identity, 95% coverage: 6:530/553 of query aligns to 2:561/565 of 5oc1A
- active site: V339 (≠ I323), N413 (≠ H378), A414 (≠ G379), I499 (≠ A469), H501 (= H471), A544 (≠ G513), H545 (≠ N514)
- binding 4-methoxybenzoic acid: Y91 (≠ G96), I356 (vs. gap), I390 (≠ M359), F396 (= F362), T412 (≠ K377), I499 (≠ A469), H501 (= H471), H545 (≠ N514)
- binding flavin-adenine dinucleotide: G8 (= G12), G10 (= G14), N11 (≠ S15), A12 (= A16), E32 (= E36), A33 (= A37), W60 (= W65), P78 (≠ G83), G80 (= G85), G85 (= G90), S86 (= S91), H90 (≠ N95), Y91 (≠ G96), V93 (≠ I98), V230 (= V223), S270 (= S255), A271 (≠ G256), G272 (= G257), F500 (≠ Y470), H545 (≠ N514), T546 (≠ I515), Q547 (≠ Y516), I550 (≠ V519)
3fimB Crystal structure of aryl-alcohol-oxidase from pleurotus eryingii (see paper)
31% identity, 95% coverage: 6:530/553 of query aligns to 2:561/565 of 3fimB
- active site: V339 (≠ I323), N413 (≠ H378), A414 (≠ G379), I499 (≠ A469), H501 (= H471), A544 (≠ G513), H545 (≠ N514)
- binding flavin-adenine dinucleotide: G8 (= G12), N11 (≠ S15), A12 (= A16), E32 (= E36), A33 (= A37), W60 (= W65), P78 (≠ G83), G80 (= G85), G85 (= G90), S86 (= S91), H90 (≠ N95), Y91 (≠ G96), V93 (≠ I98), V230 (= V223), S270 (= S255), A271 (≠ G256), F500 (≠ Y470), H501 (= H471), H545 (≠ N514), T546 (≠ I515), Q547 (≠ Y516), I550 (≠ V519)
4ynuA Crystal structure of aspergillus flavus fadgdh in complex with d- glucono-1,5-lactone (see paper)
30% identity, 95% coverage: 6:530/553 of query aligns to 4:562/569 of 4ynuA
- active site: V341 (≠ P318), F412 (≠ Q381), W413 (≠ I382), N501 (≠ A469), H503 (= H471), G545 (= G513), H546 (≠ N514)
- binding flavin-adenine dinucleotide: G12 (= G14), T13 (≠ S15), S14 (≠ A16), E34 (= E36), A35 (= A37), Y51 (≠ N60), F55 (vs. gap), W61 (= W65), R79 (≠ G83), G81 (= G85), G86 (= G90), T87 (≠ S91), N91 (= N95), G92 (= G96), T232 (≠ R222), A233 (≠ V223), A273 (≠ G256), G274 (= G257), R277 (≠ N260), F502 (≠ Y470), A536 (= A504), H546 (≠ N514), L547 (≠ I515), V548 (≠ Y516), L551 (≠ V519)
- binding D-glucono-1,5-lactone: Y51 (≠ N60), E411 (≠ Y380), A496 (≠ K464), N497 (≠ D465), R499 (≠ E467), R499 (≠ E467), N501 (≠ A469), H503 (= H471), H546 (≠ N514)
7vzsA Fad-dpendent glucose dehydrogenase complexed with an inhibitor at ph7.56
30% identity, 95% coverage: 6:530/553 of query aligns to 4:562/566 of 7vzsA
- binding D-glucal: Y6 (= Y8), L22 (= L24), N25 (≠ D27), Y51 (≠ N60), I349 (≠ M326), Q356 (= Q332), E411 (≠ Y380), E444 (≠ P416), W445 (≠ N417), K448 (= K420), R499 (≠ E467), N501 (≠ A469), H546 (≠ N514)
- binding flavin-adenine dinucleotide: G10 (= G12), G12 (= G14), T13 (≠ S15), S14 (≠ A16), E34 (= E36), A35 (= A37), Y51 (≠ N60), F55 (vs. gap), W61 (= W65), R79 (≠ G83), G81 (= G85), G86 (= G90), T87 (≠ S91), N91 (= N95), G92 (= G96), M93 (= M97), A94 (≠ I98), T232 (≠ R222), A233 (≠ V223), A273 (≠ G256), G274 (= G257), R277 (≠ N260), F502 (≠ Y470), A536 (= A504), H546 (≠ N514), L547 (≠ I515), V548 (≠ Y516), L551 (≠ V519)
Sites not aligning to the query:
4yntA Crystal structure of aspergillus flavus fad glucose dehydrogenase (see paper)
30% identity, 95% coverage: 6:530/553 of query aligns to 5:563/570 of 4yntA
- active site: V342 (≠ P318), F413 (≠ Q381), W414 (≠ I382), N502 (≠ A469), H504 (= H471), G546 (= G513), H547 (≠ N514)
- binding dihydroflavine-adenine dinucleotide: G13 (= G14), T14 (≠ S15), S15 (≠ A16), E35 (= E36), A36 (= A37), F56 (vs. gap), W62 (= W65), R80 (≠ G83), G82 (= G85), G87 (= G90), T88 (≠ S91), N92 (= N95), G93 (= G96), M94 (= M97), A95 (≠ I98), A234 (≠ V223), A274 (≠ G256), R278 (≠ N260), F503 (≠ Y470), A537 (= A504), H547 (≠ N514), L548 (≠ I515), V549 (≠ Y516), L552 (≠ V519)
E4QP00 5-(hydroxymethyl)furfural oxidase; 5-hydroxymethylfurfural oxidase; HMFO; Thiol oxidase; EC 1.1.3.47; EC 1.8.3.- from Methylovorus sp. (strain MP688) (see paper)
30% identity, 96% coverage: 1:531/553 of query aligns to 1:528/531 of E4QP00
- V101 (≠ I94) mutation to H: Abolishes activity.
- M103 (≠ G96) mutation to A: 16-fold reduction in catalytic efficiency on vanillyl alcohol.
- V367 (≠ G379) mutation to K: 1.6-fold reduction in catalytic efficiency on vanillyl alcohol. Shows significantly improved activity on the aldehyde 5-formyl-2-furancarboxylate, which results in a better 5-hydroxymethylfurfural to 2,5-furandicarboxylate conversion.; mutation to R: 1.4-fold reduction in catalytic efficiency on vanillyl alcohol. Shows significantly improved activity on the aldehyde 5-formyl-2-furancarboxylate, which results in a better 5-hydroxymethylfurfural to 2,5-furandicarboxylate conversion. Displays a catalytic efficiency toward 5-formyl-2-furancarboxylate that is over 1000-fold higher than that for wild-type; when associated with F-466.
- W369 (≠ Q381) mutation to A: 7.5-fold reduction in catalytic efficiency on vanillyl alcohol.
- V465 (≠ A469) mutation to A: 18-fold reduction in catalytic efficiency on vanillyl alcohol.
- W466 (≠ Y470) mutation to A: 39-fold reduction in catalytic efficiency on vanillyl alcohol. In contrast to wild-type, is active on secondary alcohols, such as (S)-1-phenylethanol, and is strictly enantionselective as this mutant has no activity on (R)-1-phenylethanol. Shows increased activity on the aldehyde 5-formyl-2-furancarboxylate.; mutation to F: 3.4-fold reduction in catalytic efficiency on vanillyl alcohol. In contrast to wild-type, is active on secondary alcohols, such as (S)-1-phenylethanol, and is strictly enantionselective as this mutant has no activity on (R)-1-phenylethanol. Shows increased activity on the aldehyde 5-formyl-2-furancarboxylate. Displays a catalytic efficiency toward 5-formyl-2-furancarboxylate that is over 1000-fold higher than that for wild-type; when associated with R-367.
- H467 (= H471) mutation to A: Abolishes activity.
- N511 (= N514) mutation to A: 53-fold reduction in catalytic efficiency on vanillyl alcohol.
4udqA Crystal structure of 5-hydroxymethylfurfural oxidase (hmfo) in the reduced state
30% identity, 95% coverage: 6:531/553 of query aligns to 2:524/525 of 4udqA
- active site: L331 (≠ E342), F364 (≠ Y380), W365 (≠ Q381), V461 (≠ A469), H463 (= H471), A506 (≠ G513), N507 (= N514)
- binding flavin-adenine dinucleotide: G8 (= G12), G10 (= G14), T11 (≠ S15), A12 (= A16), E32 (= E36), A33 (= A37), W64 (= W65), G88 (= G85), G93 (= G90), G94 (≠ S91), N98 (= N95), M99 (≠ G96), V101 (≠ I98), V229 (= V223), T261 (≠ S255), A262 (≠ G256), W462 (≠ Y470), H463 (= H471), A497 (= A504), N507 (= N514), T508 (≠ I515), N509 (≠ Y516), T512 (≠ V519)
6xuuA Crystallographic structure of oligosaccharide dehydrogenase from pycnoporus cinnabarinus, glucose-bound form (see paper)
29% identity, 95% coverage: 4:530/553 of query aligns to 13:585/589 of 6xuuA
- binding beta-D-glucopyranose: E35 (= E26), N67 (≠ G59), H83 (= H82), N104 (≠ P104), D108 (≠ E108), S139 (≠ T135), A140 (≠ V136), N142 (≠ D139), T166 (vs. gap), D169 (vs. gap), T188 (≠ A170), W213 (vs. gap), A225 (= A204), Y226 (= Y205), Q329 (≠ E299), N330 (≠ A300), A331 (≠ Y301), Q357 (≠ P322), F414 (vs. gap), K474 (≠ E431), N517 (≠ E467), G520 (vs. gap), A522 (vs. gap), V524 (vs. gap), H526 (= H471), H569 (≠ N514)
- binding flavin-adenine dinucleotide: G21 (= G12), G23 (= G14), L24 (≠ S15), T25 (≠ A16), E45 (= E36), A46 (= A37), F66 (≠ I58), W72 (= W65), H83 (= H82), G85 (= G85), G90 (= G90), S91 (= S91), N95 (= N95), G96 (= G96), A97 (≠ M97), A98 (≠ I98), M244 (≠ R222), A245 (≠ V223), A286 (≠ G256), Q290 (≠ N260), A525 (≠ Y470), A559 (= A504), H569 (≠ N514), L570 (≠ I515), S571 (≠ Y516), L574 (≠ V519)
- binding alpha-D-glucopyranose: G96 (= G96), F414 (vs. gap), W428 (vs. gap)
6xutA Crystallographic structure of oligosaccharide dehydrogenase from pycnoporus cinnabarinus, ligand-free form (see paper)
29% identity, 95% coverage: 4:530/553 of query aligns to 13:585/589 of 6xutA
- binding flavin-adenine dinucleotide: G21 (= G12), G23 (= G14), L24 (≠ S15), T25 (≠ A16), E45 (= E36), A46 (= A37), F66 (≠ I58), W72 (= W65), H83 (= H82), G85 (= G85), G90 (= G90), S91 (= S91), N95 (= N95), G96 (= G96), A97 (≠ M97), A98 (≠ I98), M244 (≠ R222), A245 (≠ V223), A286 (≠ G256), G287 (= G257), Q290 (≠ N260), A525 (≠ Y470), H526 (= H471), A559 (= A504), H569 (≠ N514), L570 (≠ I515), S571 (≠ Y516), L574 (≠ V519)
6h3oF Alcohol oxidase from phanerochaete chrysosporium mutant f101s (see paper)
27% identity, 97% coverage: 4:542/553 of query aligns to 4:640/650 of 6h3oF
- binding flavin-adenine dinucleotide: P15 (≠ S15), E37 (= E36), G38 (≠ A37), N90 (≠ K86), I91 (≠ V87), G94 (= G90), G95 (≠ S91), N99 (= N95), S100 (≠ G96), Q101 (≠ M97), M102 (≠ I98), R227 (= R222), V228 (= V223), S271 (≠ G256), I279 (≠ L264), W559 (≠ Y470), L593 (≠ A504), N603 (= N514), T604 (≠ I515), Y605 (= Y516), A608 (≠ V519)
6h3gB Alcohol oxidase from phanerochaete chrysosporium (see paper)
27% identity, 97% coverage: 4:542/553 of query aligns to 2:638/642 of 6h3gB
- binding flavin-adenine dinucleotide: P13 (≠ S15), E35 (= E36), G36 (≠ A37), A87 (≠ G85), G92 (= G90), G93 (≠ S91), N97 (= N95), F98 (≠ G96), M100 (≠ I98), R225 (= R222), V226 (= V223), S269 (≠ G256), I277 (≠ L264), W557 (≠ Y470), L591 (≠ A504), N601 (= N514), T602 (≠ I515), Y603 (= Y516), A606 (≠ V519)
- binding glycerol: R54 (≠ P51), Q57 (≠ L54), N59 (≠ Q56)
Query Sequence
>WP_025274241.1 NCBI__GCF_000527155.1:WP_025274241.1
MAVKKYDYIIVGGGSAGCVLANRLSEDPTNKVLMIEAGRTDWIWDIFIHMPAALTQIIGN
PLYDWRYESEPEPYMGGRRVYHGRGKVLGGSSSINGMIFQRGNPMDLERWAADPGMETWD
YAHCLPYFKRMENLTVGPDDWRSDEGPLWLERGPAANPLFKAFLKSAEQAGYPITRDVNG
YQQEGFQHFDRNIKNGRRWSSARAYYHPIKDRPNLELTTLSRVTKVLMKGKRAVGVQYRR
AGINHTVLAGEVVLSGGSINTPQLLQLSGIGDPEHLQSVGIKPRHELPGVGENLQDHLEA
YIQHASSQPVSYNPIYNPLYYPIIGMQWLWGQRGPAATNHFEAGGFVRGNDVVDYPNLMF
HFLPIAVRYDGTKVDAKHGYQIHIGPMYSDVRGDVKIKSDNPKEKPRLRFNYLSTPNDRK
EWIEAIRVGREIMAQDAFKEYDAGELSPGPGVQSDKEILDWVAKDAETAYHPSCTAKMGV
GDDAVVDPLSMKVHGLEGLRVVDASAMPYLTNGNIYAPVMMLAEKAADLVLGNTPLPASD
VPYYQHDKSASQG
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory