SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_025274361.1 NCBI__GCF_000527155.1:WP_025274361.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

A0QYS9 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
51% identity, 96% coverage: 9:392/401 of query aligns to 3:384/390 of A0QYS9

query
sites
A0QYS9

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K304 (≠ R314) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

P9WPZ7 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
50% identity, 98% coverage: 1:393/401 of query aligns to 1:395/400 of P9WPZ7

query
sites
P9WPZ7

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M1 (= M1) modified: Initiator methionine, Removed
T2 (= T2) modified: N-acetylthreonine; partial
K314 (≠ R314) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

7nncC Crystal structure of mycobacterium tuberculosis argd with prosthetic group pyridoxal-5'-phosphate and 6-methoxyquinoline-3-carboxylic acid
50% identity, 97% coverage: 5:393/401 of query aligns to 1:389/391 of 7nncC

query
sites
7nncC

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active site: F133 (= F139), D218 (= D224), K247 (= K253)
binding 6-methoxyquinoline-3-carboxylic acid: N75 (= N79), L76 (= L80)

7nn4A Crystal structure of mycobacterium tuberculosis argd with prosthetic group pyridoxal 5'-phosphate and 3-hydroxy-2-naphthoic acid.
50% identity, 97% coverage: 5:393/401 of query aligns to 1:389/391 of 7nn4A

query
sites
7nn4A

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L

H

T

G

G

K

R

T

T

R

K

I

L

V

V

A

A

E

H

N

D

S

A

F
|
F

H

H

G

G

R
|
R

T

T

L

M

G

G

T

S

L

L

A

A

V

L

T

T

G

G

K

Q

A

P

S

A

I

K

R

Q

E

T

P

P

F

F

A

A

P

P

F

L

G

P

I

G

E

D

A

V

T

T

F

H

V

V

P

G

Y

Y

G

G

D

D

T

V

A

D

A

A

L

L

T

A

A

A

V

V

D

D

T

H

V

T

A

A

V

V

I

F

L

L

E

E

P

P

T

I

Q

M

G

G

E

E

A

S

G

G

V

V

P

V

A

P

S

P

T

A

D

G

F

Y

L

L

H

A

T

A

A

A

R

R

A

D

L

I

T

T

H

A

E

R

H

R

G

G

S

A

A

L

L

L

I

V

L

L

D
|
D

E

E

I

V

Q

Q

A

T

G

G

F

M

G

G

R

R

T

T

G

G

R

A

W

F

F

F

A

A

H

H

H

Q

A

H

A

D

D

G

I

I

T

T

P

P

D

D

I

V

T

T

L

L

A

A

K
|
K

G

G

L

L

A

G

G

G

I

L

P

P

M

I

G

G

A

A

T

C

I

L

G

A

V

V

G

G

D

P

W

A

G

A

N

E

V

L

F

L

Q

T

A

P

G

G

D

L

H

H

G
|
G

S

S

T

T

F

F

G

G

N

N

P

P

I

V

A

C

G

A

A

A

L

L

A

A

V

V

I

L

D

R

I

V

I

L

E

A

R

S

E

D

D

G

L

L

F

V

D

R

N

R

V

A

N

E

T

V

L

L

G

G

R

K

K

S

L

L

E

R

N

H

R

G

L

I

H

E

-

A

-

L

N

G

H

H

P

P

A

L

V

I

I

D

A

H

V

V

R

R

G

G

E

R

A

G

L

L

W

L

R

L

G

G

I

I

V

A

L

L

D

T

T

A

D

P

I

H

A

A

A

K

D

D

V

A

C

E

G

A

Q

T

L

A

R

R

N

D

A

A

G

G

I

Y

L

L

A

V

N

N

P

A

V

A

R

A

P

P

N

D

V

V

I

I

R

R

I

L

A

A

P

P

L

L

S

I

I

I

T

A

A

E

A

A

E

Q

L

L

E

D

Q

G

F

F

T

V

S

A

A

A

L

L

T

P

D

A

V

I

L

L

D

D

Q

R

active site: F133 (= F139), D218 (= D224), K247 (= K253)
binding 3-hydroxynaphthalene-2-carboxylic acid: Y17 (= Y21), L76 (= L80), R136 (= R142), G274 (= G280)

8ht4B Crystal structure of acetylornithine aminotransferase complex with plp from corynebacterium glutamicum (see paper)
43% identity, 95% coverage: 13:391/401 of query aligns to 6:389/390 of 8ht4B

query
sites
8ht4B

Y

W

A

P

D

Q

A

V

L

I

M

I

D

N

T

T

Y

Y

G

G

Q

T

P

P

V

V

A

E

L

L

A

V

S

S

G

G

N

K

G

G

R

A

H

T

V

V

T

T

D

D

V

D

D

Q

G

G

R

N

T

V

Y

Y

L

I

D

D

M

L

I

L

G

A

G

G

I

I

A

A

V

V

S

N

T

A

V

L

G

G

H

H

R

A

H

H

P

P

D

A

Y

I

V

I

E

E

A

A

V

V

V

T

R

N

Q

Q

T

I

A

G

T

Q

I

L

A

G

H

H

T

V

S

S

N

N

L

L

F

F

F

A

H

S

P

R

T

P

E

V

V

V

E

E

L

V

A

A

E

E

T

E

L

L

V

I

K

K

L

R

T

F

G

S

A

L

D

D

G

D

-

A

-

T

-

L

-

A

-

Q

-

T

R

R

V

V

F

F

S

C

N

N

S
|
S

G
|
G

T
x
A

E

E

A

A

N

N

E

E

A

A

L

F

K

K

L

I

A

A

L

-

K

-

A

-

G

-

K

R

P

L

H

T

G

G

K

R

T

S

R

R

I

I

V

L

A

A

E

V

N

H

S

G

F
|
F

H
|
H

G

G

R

R

T

T

L

M

G

G

T

S

L

L

A

A

V

L

T

T

G

G

K

Q

A

P

S

D

I

K

R

R

E

E

P

A

F

F

A

L

P

P

F

M

G

P

I

S

E

G

A

V

T

E

F

F

V

Y

P

P

Y

Y

G

G

D

D

T

T

A

D

A

Y

L

L

T

R

A

K

A

M

V

V

D

E

D

T

T

N

-

P

-

T

-

D

V

V

A

A

V

I

I

F

L

L

E
|
E

P

P

T

I

Q

Q

G

G

E

E

A

T

G

G

V

V

P

P

A

A

S

P

T

E

D

G

F

F

L

L

H

K

T

A

A

V

R

R

A

E

L

L

T

C

H

D

E

E

H

Y

G

G

S

I

A

L

L

M

I

I

L

T

D
|
D

E

E

I
x
V

Q
|
Q

A

T

G

G

F

V

G

G

R

R

T

T

G

G

R

D

W

F

F

F

A

A

H

H

H

Q

A

H

A

D

D

G

I

V

T

V

P

P

D

D

I

V

T

T

L

M

A

A

K
|
K

G

G

L

L

A

G

G

G

I

L

P

P

M

I

G

G

A

A

T

C

I

L

G

A

V

T

G

G

D

R

W

A

G

A

N

E

V

L

F

M

Q

T

A

P

G

G

D

K

H

H

G

G

S

T

T

T

F

F

G

G

N

N

P

P

I

V

A

A

G

C

A

A

L

K

A

A

V

V

I

L

D

S

I

V

E

D

R

-

E

D

D

A

L

F

F

C

D

A

N

E

V

V

N

A

T

R

L

K

G

G

R

E

K

L

L

F

E

K

N

E

R

L

L

L

H

A

N

K

H

V

P

D

A

G

V

V

I

V

A

D

V

V

R

R

G

G

E

R

A

G

L

L

W

M

R

L

G

G

I

V

V

V

L

L

D

E

T

R

D

D

I

V

A

A

A

K

D

Q

V

A

C

V

G

L

Q

D

L

G

R

F

N

K

A

H

G

G

I

V

L

I

A

L

N

N

P

A

V

P

R

A

P

D

N

N

V

I

I

I

R

R

I

L

A

T

P

P

L

L

S

V

I

I

T

T

A

D

A

E

E

E

L

I

E

A

Q

D

F

A

T

V

S

K

A

A

L

I

T

A

D

E

V

T

L

I

binding pyridoxal-5'-phosphate: S108 (= S108), G109 (= G109), A110 (≠ T110), F135 (= F139), H136 (= H140), E190 (= E191), D223 (= D224), V225 (≠ I226), Q226 (= Q227), K252 (= K253)

2ordA Crystal structure of acetylornithine aminotransferase (ec 2.6.1.11) (acoat) (tm1785) from thermotoga maritima at 1.40 a resolution
41% identity, 94% coverage: 17:392/401 of query aligns to 11:391/393 of 2ordA

query
sites
2ordA

L

L

M

M

D

N

T

T

Y

Y

G

S

Q

R

P

F

P

P

V

A

A

T

L

F

A

V

S

Y

G

G

N

K

G

G

R

S

H

W

V

I

T

Y

D

D

V

E

D

K

G

G

R

N

T

A

Y

Y

L

L

D

D

M

F

I

T

G

S

G

G

I

I

A

A

V

V

S

N

T

V

V

L

G

G

H

H

R

S

H

H

P

P

D

R

Y

L

V

V

E

E

A

A

V

I

V

K

R

D

Q

Q

T

A

A

E

T

K

I

L

A

I

H

H

T

C

S

S

N

N

L

L

F

F

F

W

H

N

P

R

T

P

E

Q

V

M

E

E

L

L

A

A

E

E

T

L

L

L

V

S

K

K

L

N

T

T

G

F

A

G

D

-

G

G

R

K

V

V

F

F

S

A

N

N

S

T

G
|
G

T
|
T

E

E

A

A

N

N

E

E

A

A

L

I

K

K

L

I

A

A

-

R

-

K

L

Y

K

G

A

K

G

K

K

K

P

S

H

E

G

K

K

K

T

Y

R

R

I

I

V

L

A

S

A

A

E

H

N

N

S

S

F
|
F

H
|
H

G

G

R

R

T

T

L

L

G

G

T

S

L

L

A

T

V

A

T

T

G

G

K

Q

A

P

S

K

I

Y

R

Q

E

K

P

P

F

F

A

E

P

P

F

L

G

V

I

P

E

G

A

F

T

E

F

Y

V

F

P

E

Y

F

G

N

D

N

T

V

A

E

A

D

L

L

T

R

A

R

A

K

V

M

D

S

D

E

T

D

V

V

A

C

A

A

V

V

I

F

L

L

E
|
E

P

P

T

I

Q

Q

G

G

E

E

A

S

G

G

V

I

V

V

P

P

A

A

S

T

T

K

D

E

F

F

L

L

H

E

T

E

A

A

R

R

A

K

L

L

T

C

H

D

E

E

H

Y

G

D

S

A

A

L

L

L

I

V

L

F

D
|
D

E

E

I
x
V

Q
|
Q

A

C

G

G

F

M

G

G

R

R

T

T

G

G

R

K

W

L

F

F

A

A

H

Y

H

Q

A

K

A

Y

D

G

I

V

T

V

P

P

D

D

I

V

I

L

T

T

L

T

A

A

K
|
K

G

G

L

L

A

G

G

G

I

V

P

P

M

I

G

G

A

A

T

V

I

I

G

-

V

V

G

N

D

E

W

R

G

A

N

N

V

V

F

L

Q

E

A

P

G

G

D

D

H

H

G

G

S

T

T
|
T

F

F

G

G

N

N

P

P

I

L

A

A

G

C

A

R

A

A

A

G

L

V

A

T

V

V

I

I

D

K

I

E

I

L

E

T

R

K

E

E

D

G

L

F

F

L

D

E

N

E

V

V

-

E

-

K

-

G

N

N

T

Y

L

L

G

M

R

K

K

K

L

L

E

Q

N

E

R

M

L

K

H

E

N

E

H

Y

P

D

A

V

V

V

I

A

A

D

V

V

R

R

G

G

E

M

A

G

L

L

W

M

R

I

G

G

I

I

V

Q

L

F

D

R

T

E

D

E

I

V

A

S

-

N

A

R

D

E

V

V

C

A

G

T

Q

K

L

C

R

F

N

E

A

N

G

K

I

L

L

L

A

V

N

V

P

P

V

A

R

G

P

N

N

N

V

T

I

I

R
|
R

I

F

A

L

P

P

L

L

S

T

I

V

T

E

A

Y

A

G

E

E

L

I

E

D

Q

L

F

A

T

V

S

E

A

T

L

L

T

K

D

K

V

V

L

L

D

Q

active site: F134 (= F139), E186 (= E191), D219 (= D224), Q222 (= Q227), K248 (= K253), T276 (= T282), R367 (= R368)
binding pyridoxal-5'-phosphate: G102 (= G109), T103 (= T110), F134 (= F139), H135 (= H140), E186 (= E191), D219 (= D224), V221 (≠ I226), Q222 (= Q227), K248 (= K253)

Q9X2A5 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)
41% identity, 94% coverage: 17:392/401 of query aligns to 3:383/385 of Q9X2A5

query
sites
Q9X2A5

L

L

M

M

D

N

T

T

Y

Y

G

S

Q

R

P

F

P

P

V

A

A

T

L

F

A

V

S

Y

G

G

N

K

G

G

R

S

H

W

V

I

T

Y

D

D

V

E

D

K

G

G

R

N

T

A

Y

Y

L

L

D

D

M

F

I

T

G

S

G

G

I

I

A

A

V

V

S

N

T

V

V

L

G

G

H

H

R

S

H

H

P

P

D

R

Y

L

V

V

E

E

A

A

V

I

V

K

R

D

Q

Q

T

A

A

E

T

K

I

L

A

I

H

H

T

C

S

S

N

N

L

L

F

F

F

W

H

N

P

R

T

P

E

Q

V

M

E

E

L

L

A

A

E

E

T

L

L

L

V

S

K

K

L

N

T

T

G

F

A

G

D

-

G

G

R

K

V

V

F

F

S

A

N

N

S

T

G
|
G

T
|
T

E

E

A

A

N

N

E

E

A

A

L

I

K

K

L

I

A

A

-

R

-

K

L

Y

K

G

A

K

G

K

K

K

P

S

H

E

G

K

K

K

T

Y

R

R

I

I

V

L

A

S

A

A

E

H

N

N

S

S

F

F

H

H

G

G

R

R

T

T

L

L

G

G

T

S

L

L

A

T

V

A

T

T

G

G

K

Q

A

P

S

K

I

Y

R

Q

E

K

P

P

F

F

A

E

P

P

F

L

G

V

I

P

E

G

A

F

T

E

F

Y

V

F

P

E

Y

F

G

N

D

N

T

V

A

E

A

D

L

L

T

R

A

R

A

K

V

M

D

S

D

E

T

D

V

V

A

C

A

A

V

V

I

F

L

L

E

E

P

P

T

I

Q

Q

G

G

E

E

A

S

G

G

V

I

V

V

P

P

A

A

S

T

T

K

D

E

F

F

L

L

H

E

T

E

A

A

R

R

A

K

L

L

T

C

H

D

E

E

H

Y

G

D

S

A

A

L

L

L

I

V

L

F

D

D

E

E

I

V

Q

Q

A

C

G

G

F

M

G

G

R

R

T

T

G

G

R

K

W

L

F

F

A

A

H

Y

H

Q

A

K

A

Y

D

G

I

V

T

V

P

P

D

D

I

V

I

L

T

T

L

T

A

A

K

K

G

G

L

L

A

G

G

G

I

V

P

P

M

I

G

G

A

A

T

V

I

I

G

-

V

V

G

N

D

E

W

R

G

A

N

N

V

V

F

L

Q

E

A

P

G

G

D

D

H

H

G

G

S

T

T
|
T

F

F

G

G

N

N

P

P

I

L

A

A

G

C

A

R

A

A

A

G

L

V

A

T

V

V

I

I

D

K

I

E

I

L

E

T

R

K

E

E

D

G

L

F

F

L

D

E

N

E

V

V

-

E

-

K

-

G

N

N

T

Y

L

L

G

M

R

K

K

K

L

L

E

Q

N

E

R

M

L

K

H

E

N

E

H

Y

P

D

A

V

V

V

I

A

A

D

V

V

R

R

G

G

E

M

A

G

L

L

W

M

R

I

G

G

I

I

V

Q

L

F

D

R

T

E

D

E

I

V

A

S

-

N

A

R

D

E

V

V

C

A

G

T

Q

K

L

C

R

F

N

E

A

N

G

K

I

L

L

L

A

V

N

V

P

P

V

A

R

G

P

N

N

N

V

T

I

I

R

R

I

F

A

L

P

P

L

L

S

T

I

V

T

E

A

Y

A

G

E

E

L

I

E

D

Q

L

F

A

T

V

S

E

A

T

L

L

T

K

D

K

V

V

L

L

D

Q

GT 94:95 (= GT 109:110) binding pyridoxal 5'-phosphate
T268 (= T282) binding pyridoxal 5'-phosphate

Q9M8M7 Acetylornithine aminotransferase, chloroplastic/mitochondrial; ACOAT; Acetylornithine transaminase; AOTA; Protein HOPW1-1-INTERACTING 1; EC 2.6.1.11 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
42% identity, 94% coverage: 14:391/401 of query aligns to 66:453/457 of Q9M8M7

query
sites
Q9M8M7

A

A

D

K

A

V

L

I

M

V

D

G

T

T

Y

Y

G

A

Q

R

P

A

P

P

V

V

A

V

L

L

A

S

S

S

G

G

N

K

G

G

R

C

H

K

V

L

T

F

D

D

V

P

D

E

G

G

R

K

T

E

Y

Y

L

L

D

D

M

C

I

A

G

S

G

G

I

I

A

A

V

V

S

N

T

A

V

L

G

G

H

H

R

G

H

D

P

P

D

D

Y

W

V

L

E

R

A

A

V

V

V

T

R

E

Q

Q

T

A

A

G

T

V

I

L

A

A

H

H

T

V

S

S

N

N

L

V

F

Y

H

T

P

I

T

P

E

Q

V

I

E

E

L

L

A

A

E

K

T

R

L

L

V

V

K

A

L

S

T

S

G

F

A

A

D

D

G

-

R

R

V

V

F

F

S

C

N

N

S

S

G

G

T

T

E

E

A

A

N

N

E

E

A

A

L

I

K

K

L

F

A

S

L

R

K

K

A

F

G

Q

K

R

-

F

-

T

-

H

P

P

H

E

G

D

K

K

-

E

-

V

-

A

T

T

R

G

I

F

V

I

A

A

A

F

E

T

N

N

S

S

F

F

H

H

G

G

R

R

T

T

L

L

G

G

T

A

L

L

A

A

V

L

T

T

G

S

K

K

A

E

S

Q

I

Y

R

R

E

T

P

P

F

F

A

E

P

P

F

I

G

M

I

P

E

G

A

V

T

T

F

F

V

L

P

E

Y

Y

G

G

D

N

T

I

A

Q

A

A

L

A

T

T

A

D

A

L

V

I

D

R

D

S

-

G

T

K

V

I

A

A

V

V

I

F

L

V

E

E

P

P

T

I

Q

Q

G

G

E

E

A

G

G

G

V

I

V

Y

P

S

A

A

S

T

T

K

D

E

F

F

L

L

H

Q

T

S

A

L

R

R

A

S

L

A

T

C

H

D

E

A

H

A

G

G

S

S

A

L

L

L

I

V

L

F

D

D

E

E

I

V

Q

Q

A

C

G

G

F

L

G

G

R

R

T

T

G

G

R

L

W

M

F

W

A

A

H

Y

H

E

A

A

A

F

D

G

I

V

T

T

P

P

D

D

I

I

I

M

T

T

L

V

A

A

K

K

G

P

L

L

A

A

G

G

I

L

P

P

M

I

G

G

A

A

T

V

I

L

G

V

V

T

G

E

D

K

W

V

G

A

N

E

V

T

F

I

Q

N

A

Y

G

G

D

D

H

H

G

G

S

S

T

T

F

F

G

A

G

G

N

S

P

P

I

L

A

V

G

C

A

S

A

A

L

I

A

A

V

V

I

M

D

D

I

K

I

V

E

S

R

K

E

P

D

S

L

F

F

L

D

S

N

S

V

V

N

S

T

N

L

K

G

G

R

R

K

Y

-

F

-

R

-

D

-

L

L

L

E

V

N

K

R

K

L

L

H

G

N

G

H

N

P

S

A

H

V

V

I

K

A

E

V

V

R

R

G

G

E

E

A

G

L

L

W

I

R

I

G

G

I

V

V

E

L

L

D

D

T

V

D

P

I

-

A

A

A

S

D

S

V

L

C

V

G

D

Q

A

L

C

R

R

N

D

A

S

G

G

I

L

L

L

A

I

N

L

P

T

V

A

-

G

R

K

P

G

N

N

V

V

I

V

R

R

I

I

A

V

P

P

L

L

S

V

I

I

T

S

A

E

E

E

L

I

E

E

Q

R

F

A

T

V

S

E

A

I

L

M

T

S

D

Q

V

N

L

L

Sites not aligning to the query:

1:41 modified: transit peptide, Chloroplast and mitochondrion
42 modified: N-acetylvaline; in Acetylornithine aminotransferase, chloroplastic

P73133 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Synechocystis sp. (strain PCC 6803 / Kazusa) (see paper)
40% identity, 95% coverage: 2:382/401 of query aligns to 17:416/429 of P73133

query
sites
P73133

T

V

T

T

D

D

S

L

T

S

T

P

A

A

L

A

Q

E

N

F

R

K

Y

T

A

A

D

D

-

F

-

D

-

T

A

Y

L

V

M

M

D

N

T

T

Y
|
Y

G

G

Q

R

P

F

P

P

V

I

A

A

L

I

A

A

S

R

G

G

N

Q

G

G

R

S

H

T

V

L

T

W

D

D

V

T

D

E

G

G

R

K

T

S

Y

Y

L

L

D

D

M

F

I

V

G

A

G

G

I

I

A

A

V

T

S

C

T

T

V

L

G

G

H

H

R

A

H

H

P

P

D

A

Y

L

V

V

E

R

A

A

V

V

V

S

R

D

Q

Q

T

I

A

Q

T

K

I

L

A

H

H

H

T

V

S

S

N

N

L

L

F

Y

H

I

P

P

T

E

E

Q

V

G

E

E

L

L

A

A

E

K

T

W

L

I

V

V

K

E

L

H

T

S

G

C

A

A

D

D

G

-

R

R

V

V

F

F

S

C

N

N

S
|
S

G
|
G

T
x
A

E

E

A

A

N

N

E

E

A

A

L

I

K

K

L

L

A

V

L

R

K

K

A

Y

G

A

K

H

P

T

-

V

-

L

-

D

-

F

H

L

G

E

K

Q

T

P

R

V

I

I

V

L

A

T

A

A

E

K

N

A

S

S

F

F

H

H

G

G

R
|
R

T

T

L

L

G

A

T

T

L

I

A

T

V

A

T

T

G

G

K

Q

A

P

S

K

I

Y

R

Q

E

Q

P

Y

F

F

A

D

P

P

F

L

G

V

I

P

E

G

A

F

T

D

F

Y

V

V

P

P

Y

Y

G

N

D

D

T

I

A

R

A

S

L

L

T

E

A

N

A

K

V

V

D

A

D

D

T

L

-

D

-

E

-

G

-

N

-

S

-

R

V

V

A

A

V

I

I

F

L

L

E

E

P

P

T

L

Q

Q

G

G

E
|
E

A

G

G

G

V

V

V

R

P

P

A

G

S

D

T

L

D

A

F

Y

L

F

H

K

T

R

A

V

R

R

A

E

L

I

T

C

H

D

E

Q

H

N

G

D

S

I

A

L

L

L

I

V

L

F

D
|
D

E

E

I

V

Q
|
Q

A

V

G

G

F

V

G

G

R

R

T

T

G

G

R

K

W

L

F

W

A

G

H

Y

H

E

A

H

A

L

D

G

I

V

T

E

P

P

D

D

I

I

I

F

T

T

L

S

A

A

K
|
K

G

G

L

L

A

A

G

G

I

V

P

P

M

I

G

G

A

A

T

M

I

M

G

-

V

C

G

K

D

K

W

F

G

C

N

D

V

V

F

F

Q

E

A

P

G

G

D

N

H

H

G

A

S

S

T
|
T

F

F

G

G

N

N

P

P

I

L

A

A

G

C

A

A

A

G

L

L

A

A

V

V

I

L

D

K

I

T

I

I

E

E

R

G

E

D

D

R

L

L

F

L

D

D

N

N

V

V

N

Q

T

A

L

R

G

G

R

E

K

Q

L

L

E

R

N

S

R

G

L

L

-

A

-

E

-

I

-

K

H

N

N

Q

H

Y

P

P

A

T

V

L

I

F

A

T

-

E

V

V

R

R

G

G

E

W

A

G

L

L

W

I

R

N

G

G

I

L

V

E

L

I

D

S

T

A

D

E

-

S

-

L

I

T

A

S

A

V

D

E

V

I

C

V

G

K

Q

A

L

A

R

M

N

E

A

Q

G

G

I

L

L

L

A

L

N

A

P

P

V

A

R

G

P

P

N

K

V

V

I

L

R
|
R

I

F

A

V

P

P

L

L

S

V

I

V

T

T

A

E

A

A

E

E

L

I

E

A

Q

Q

Y39 (= Y21) mutation to F: Retains 7.4% of N-acetylornithine aminotransferase wild-type activity.
S125 (= S108) mutation to A: Retains 14% of N-acetylornithine aminotransferase wild-type activity.
G126 (= G109) mutation to A: Loss of N-acetylornithine aminotransferase activity.
A127 (≠ T110) mutation to S: Retains 9.1% of N-acetylornithine aminotransferase wild-type activity.
R163 (= R142) mutation to A: 4100-fold increase in KM for acetylornithine, but does not affect KM for 2-oxoglutarate. Retains 9.5% of N-acetylornithine aminotransferase wild-type activity.
E223 (= E196) mutation to A: 65-fold increase in KM for acetylornithine, but does not affect KM for 2-oxoglutarate. Retains 50% of N-acetylornithine aminotransferase wild-type activity.; mutation to S: 73-fold increase in KM for acetylornithine, but does not affect KM for 2-oxoglutarate. Shows 160% of N-acetylornithine aminotransferase wild-type activity.
D251 (= D224) mutation to A: Loss of N-acetylornithine aminotransferase activity.; mutation to E: Retains 20% of N-acetylornithine aminotransferase wild-type activity.
Q254 (= Q227) mutation to A: Retains 0.9% of N-acetylornithine aminotransferase wild-type activity.
K280 (= K253) mutation to A: Loss of N-acetylornithine aminotransferase activity.
T308 (= T282) mutation to A: Retains 0.3% of N-acetylornithine aminotransferase wild-type activity.
R402 (= R368) mutation to A: 2080-fold increase in KM for acetylornithine, but does not affect KM for 2-oxoglutarate. Retains 18% of N-acetylornithine aminotransferase wild-type activity.

Q5SHH5 [LysW]-aminoadipate semialdehyde transaminase; EC 2.6.1.118 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
42% identity, 94% coverage: 14:391/401 of query aligns to 15:394/395 of Q5SHH5

query
sites
Q5SHH5

A

A

D

E

A

K

L

T

M

L

D

D

T

S

-

G

-

V

Y

Y

G

N

Q

K

P

H

P

D

V

L

A

L

L

I

A

V

S

R

G

G

N

Q

G

G

R

A

H

R

V

V

T

W

D

D

V

A

D

E

G

G

R

N

T

E

Y

Y

L

I

D

D

M

C

I

V

G

G

I

Y

A

G

V

V

S

A

T

N

V

L

G

G

H

H

R

G

H

N

P

P

D

E

Y

V

V

V

E

E

A

A

V

V

V

K

R

R

Q

Q

T

A

A

E

T

T

I

L

A

M

H

A

T

M

S

P

N

Q

L

T

F

L

F

P

H

T

P

P

T

M

E

R

V

G

E

E

L

F

A

Y

E

R

T

T

L

L

V

T

K

A

L

I

T

L

G

P

A

P

D

E

-

L

G

N

R

R

V

V

F

F

F

P

S

V

N

N

S

S

G
|
G

T
|
T

E

E

A

A

N

N

E

E

A

A

L

L

K

K

L

F

A

A

L

-

K

-

A

-

G

-

K

R

P

A

H

H

-

T

G

G

K

R

T

K

R

K

I

F

V

V

A

A

E

M

N

R

S

G

F

F

H

S

G

G

R

R

T

T

L

M

G

G

T

S

L

L

A

S

V

V

T

T

G

W

K

E

A

P

S

K

I

Y

R

R

E

E

P

P

F

F

A

L

P

P

F

L

G

V

I

E

E

P

A

V

T

E

F

F

V

I

P

P

Y

Y

G

N

D

D

T

V

A

E

A

A

L

L

T

K

A

R

A

A

V

V

D

D

D

E

T

E

V

T

A

A

V

V

I

I

L

L

E

E

P

P

T

V

Q

Q

G

G

E

E

A

G

G

G

V

V

V

R

P

P

A

A

S

T

T

P

D

E

F

F

L

L

H

R

T

A

A

A

R

R

A

E

L

I

T

T

H

Q

E

E

H

K

G

G

S

A

A

L

L

L

I

I

L

L

D

D

E

E

I

I

Q

Q

A

T

G

G

F

M

G

G

R

R

T

T

G

G

R

K

W

R

F

F

A

A

H

F

H

E

A

H

A

F

D

G

I

I

T

V

P

P

D

D

I

I

I

L

T

T

L

L

A

A

K
|
K

G

A

L

L

A

G

G

G

I

V

P

P

M

L

G

G

A

A

T

A

I

V

G

M

V

R

G

E

D

E

W

V

G

A

N

R

V

S

F

M

Q

P

A

K

G

G

D

G

H

H

G

G

S

T

T
|
T

F

F

G

G

N

N

P

P

I

L

A

A

G

M

A

A

A

G

L

V

A

A

V

A

I

I

D

R

I

Y

I

L

E

E

R

R

E

T

D

R

L

L

F

W

D

E

N

R

V

A

N

A

T

E

L

L

G

G

R

P

K

W

L

F

E

M

N

E

R

K

L

L

H

R

-

A

-

I

N

P

H

S

P

P

A

K

V

I

I

R

A

E

V

V

R

R

G

G

E

M

A

G

L

L

W

M

R

V

G

G

I

L

V

E

L

L

D

K

T

E

D

K

I

A

A

A

A

P

D

Y

V

I

C

A

G

R

Q

L

L

E

R

K

N

E

A

H

G

R

I

V

L

L

A

A

N

L

P

Q

V

A

R

G

P

P

N

T

V

V

I

I

R

R

I

F

A

L

P

P

L

L

S

V

I

I

T

E

A

K

A

E

E

D

L

L

E

E

Q

R

F

V

T

V

S

E

A

A

L

V

T

R

D

A

V

V

L

L

GT 113:114 (= GT 109:110) binding pyridoxal 5'-phosphate
K254 (= K253) modified: N6-(pyridoxal phosphate)lysine
T283 (= T282) binding pyridoxal 5'-phosphate

1wkhA Acetylornithine aminotransferase from thermus thermophilus hb8
42% identity, 94% coverage: 14:391/401 of query aligns to 7:386/387 of 1wkhA

query
sites
1wkhA

A

A

D

E

A

K

L

T

M

L

D

D

T
x
S

-

G

-

V

Y

Y

G

N

Q

K

P

H

P

D

V

L

A

L

L

I

A

V

S

R

G

G

N

Q

G

G

R

A

H

R

V

V

T

W

D

D

V

A

D

E

G

G

R

N

T

E

Y

Y

L

I

D

D

M

C

I

V

G

G

I
x
Y

A

G

V

V

S

A

T

N

V

L

G

G

H

H

R

G

H

N

P

P

D

E

Y

V

V

V

E

E

A

A

V

V

V

K

R

R

Q

Q

T

A

A

E

T

T

I

L

A

M

H

A

T

M

S

P

N

Q

L

T

F

L

F

P

H

T

P

P

T

M

E

R

V

G

E

E

L

F

A

Y

E

R

T

T

L

L

V

T

K

A

L

I

T

L

G

P

A

P

D

E

-

L

G

N

R

R

V

V

F

F

F

P

S

V

N

N

S
|
S

G
|
G

T
|
T

E

E

A

A

N

N

E

E

A

A

L

L

K

K

L

F

A

A

L

-

K

-

A

-

G

-

K

R

P

A

H

H

-

T

G

G

K

R

T

K

R

K

I

F

V

V

A

A

E

M

N

R

S

G

F
|
F

H
x
S

G

G

R

R

T

T

L

M

G

G

T

S

L

L

A

S

V

V

T

T

G

W

K

E

A

P

S

K

I

Y

R

R

E

E

P

P

F

F

A

L

P

P

F

L

G

V

I

E

E

P

A

V

T

E

F

F

V

I

P

P

Y

Y

G

N

D

D

T

V

A

E

A

A

L

L

T

K

A

R

A

A

V

V

D

D

D

E

T

E

V

T

A

A

V

V

I

I

L

L

E
|
E

P

P

T

V

Q

Q

G

G

E
|
E

A

G

G

G

V

V

V

R

P

P

A

A

S

T

T

P

D

E

F

F

L

L

H

R

T

A

A

A

R

R

A

E

L

I

T

T

H

Q

E

E

H

K

G

G

S

A

A

L

L

L

I

I

L

L

D
|
D

E

E

I
|
I

Q
|
Q

A

T

G

G

F

M

G

G

R

R

T

T

G

G

R

K

W

R

F

F

A

A

H

F

H

E

A

H

A

F

D

G

I

I

T

V

P

P

D

D

I

I

I

L

T

T

L

L

A

A

K
|
K

G

A

L

L

A

G

G

G

I

V

P

P

M

L

G

G

A

V

T

A

I

V

G

M

V

R

G

E

D

E

W

V

G

A

N

R

V

S

F

M

Q

P

A

K

G

G

D

G

H

H

G

G

S

T

T
|
T

F

F

G

G

N

N

P

P

I

L

A

A

G

M

A

A

A

G

L

V

A

A

V

A

I

I

D

R

I

Y

I

L

E

E

R

R

E

T

D

R

L

L

F

W

D

E

N

R

V

A

N

A

T

E

L

L

G

G

R

P

K

W

L

F

E

M

N

E

R

K

L

L

H

R

-

A

-

I

N

P

H

S

P

P

A

K

V

I

I

R

A

E

V

V

R

R

G

G

E

M

A

G

L

L

W

M

R

V

G

G

I

L

V

E

L

L

D

K

T

E

D

K

I

A

A

A

A

P

D

Y

V

I

C

A

G

R

Q

L

L

E

R

K

N

E

A

H

G

R

I

V

L

L

A

A

N

L

P

Q

V

A

R

G

P

P

N

T

V

V

I

I

R
|
R

I

F

A

L

P

P

L

L

S

V

I

I

T

E

A

K

A

E

E

D

L

L

E

E

Q

R

F

V

T

V

S

E

A

A

L

V

T

R

D

A

V

V

L

L

active site: S13 (≠ T20), F132 (= F139), E184 (= E191), D217 (= D224), Q220 (= Q227), K246 (= K253), T275 (= T282), R363 (= R368)
binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: Y46 (≠ I51), S104 (= S108), G105 (= G109), T106 (= T110), F132 (= F139), S133 (≠ H140), E184 (= E191), E189 (= E196), D217 (= D224), I219 (= I226), K246 (= K253), R363 (= R368)

1wkgA Acetylornithine aminotransferase from thermus thermophilus hb8
42% identity, 94% coverage: 14:391/401 of query aligns to 7:386/387 of 1wkgA

query
sites
1wkgA

A

A

D

E

A

K

L

T

M

L

D

D

T
x
S

-

G

-

V

Y
|
Y

G

N

Q

K

P

H

P

D

V

L

A

L

L

I

A

V

S

R

G

G

N

Q

G

G

R

A

H

R

V

V

T

W

D

D

V

A

D

E

G

G

R

N

T

E

Y

Y

L

I

D

D

M

C

I

V

G

G

I
x
Y

A

G

V

V

S

A

T

N

V

L

G

G

H

H

R

G

H

N

P

P

D

E

Y

V

V

V

E

E

A

A

V

V

V

K

R

R

Q

Q

T

A

A

E

T

T

I

L

A

M

H

A

T

M

S

P

N

Q

L

T

F

L

F

P

H

T

P

P

T

M

E

R

V

G

E

E

L

F

A

Y

E

R

T

T

L

L

V

T

K

A

L

I

T

L

G

P

A

P

D

E

-

L

G

N

R

R

V

V

F

F

F

P

S

V

N

N

S

S

G
|
G

T
|
T

E

E

A

A

N

N

E

E

A

A

L

L

K

K

L

F

A

A

L

-

K

-

A

-

G

-

K

R

P

A

H

H

-

T

G

G

K

R

T

K

R

K

I

F

V

V

A

A

E

M

N

R

S

G

F
|
F

H
x
S

G

G

R
|
R

T

T

L

M

G

G

T

S

L

L

A

S

V

V

T

T

G

W

K

E

A

P

S

K

I

Y

R

R

E

E

P

P

F

F

A

L

P

P

F

L

G

V

I

E

E

P

A

V

T

E

F

F

V

I

P

P

Y

Y

G

N

D

D

T

V

A

E

A

A

L

L

T

K

A

R

A

A

V

V

D

D

D

E

T

E

V

T

A

A

V

V

I

I

L

L

E
|
E

P

P

T

V

Q

Q

G

G

E

E

A

G

G

G

V

V

V

R

P

P

A

A

S

T

T

P

D

E

F

F

L

L

H

R

T

A

A

A

R

R

A

E

L

I

T

T

H

Q

E

E

H

K

G

G

S

A

A

L

L

L

I

I

L

L

D
|
D

E

E

I
|
I

Q
|
Q

A

T

G

G

F

M

G

G

R

R

T

T

G

G

R

K

W

R

F

F

A

A

H

F

H

E

A

H

A

F

D

G

I

I

T

V

P

P

D

D

I

I

I

L

T

T

L

L

A

A

K
|
K

G

A

L

L

A

G

G

G

I

V

P

P

M

L

G

G

A

V

T

A

I

V

G

M

V

R

G

E

D

E

W

V

G

A

N

R

V

S

F

M

Q

P

A

K

G

G

D

G

H

H

G
|
G

S
x
T

T
|
T

F

F

G

G

N

N

P

P

I

L

A

A

G

M

A

A

A

G

L

V

A

A

V

A

I

I

D

R

I

Y

I

L

E

E

R

R

E

T

D

R

L

L

F

W

D

E

N

R

V

A

N

A

T

E

L

L

G

G

R

P

K

W

L

F

E

M

N

E

R

K

L

L

H

R

-

A

-

I

N

P

H

S

P

P

A

K

V

I

I

R

A

E

V

V

R

R

G

G

E

M

A

G

L

L

W

M

R

V

G

G

I

L

V

E

L

L

D

K

T

E

D

K

I

A

A

A

A

P

D

Y

V

I

C

A

G

R

Q

L

L

E

R

K

N

E

A

H

G

R

I

V

L

L

A

A

N

L

P

Q

V

A

R

G

P

P

N

T

V

V

I

I

R
|
R

I

F

A

L

P

P

L

L

S

V

I

I

T

E

A

K

A

E

E

D

L

L

E

E

Q

R

F

V

T

V

S

E

A

A

L

V

T

R

D

A

V

V

L

L

active site: S13 (≠ T20), F132 (= F139), E184 (= E191), D217 (= D224), Q220 (= Q227), K246 (= K253), T275 (= T282), R363 (= R368)
binding n~2~-acetyl-n~5~-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-l-ornithine: Y16 (= Y21), Y46 (≠ I51), G105 (= G109), T106 (= T110), F132 (= F139), S133 (≠ H140), R135 (= R142), E184 (= E191), D217 (= D224), I219 (= I226), Q220 (= Q227), K246 (= K253), G273 (= G280), T274 (≠ S281), T275 (= T282)

1vefA Acetylornithine aminotransferase from thermus thermophilus hb8
42% identity, 94% coverage: 14:391/401 of query aligns to 7:386/387 of 1vefA

query
sites
1vefA

A

A

D

E

A

K

L

T

M

L

D

D

T
x
S

-

G

-

V

Y

Y

G

N

Q

K

P

H

P

D

V

L

A

L

L

I

A

V

S

R

G

G

N

Q

G

G

R

A

H

R

V

V

T

W

D

D

V

A

D

E

G

G

R

N

T

E

Y

Y

L

I

D

D

M

C

I

V

G

G

I

Y

A

G

V

V

S

A

T

N

V

L

G

G

H

H

R

G

H

N

P

P

D

E

Y

V

V

V

E

E

A

A

V

V

V

K

R

R

Q

Q

T

A

A

E

T

T

I

L

A

M

H

A

T

M

S

P

N

Q

L

T

F

L

F

P

H

T

P

P

T

M

E

R

V

G

E

E

L

F

A

Y

E

R

T

T

L

L

V

T

K

A

L

I

T

L

G

P

A

P

D

E

-

L

G

N

R

R

V

V

F

F

F

P

S

V

N

N

S

S

G
|
G

T
|
T

E

E

A

A

N

N

E

E

A

A

L

L

K

K

L

F

A

A

L

-

K

-

A

-

G

-

K

R

P

A

H

H

-

T

G

G

K

R

T

K

R

K

I

F

V

V

A

A

E

M

N

R

S

G

F
|
F

H
x
S

G

G

R

R

T

T

L

M

G

G

T

S

L

L

A

S

V

V

T

T

G

W

K

E

A

P

S

K

I

Y

R

R

E

E

P

P

F

F

A

L

P

P

F

L

G

V

I

E

E

P

A

V

T

E

F

F

V

I

P

P

Y

Y

G

N

D

D

T

V

A

E

A

A

L

L

T

K

A

R

A

A

V

V

D

D

D

E

T

E

V

T

A

A

V

V

I

I

L

L

E
|
E

P

P

T

V

Q

Q

G

G

E

E

A

G

G

G

V

V

V

R

P

P

A

A

S

T

T

P

D

E

F

F

L

L

H

R

T

A

A

A

R

R

A

E

L

I

T

T

H

Q

E

E

H

K

G

G

S

A

A

L

L

L

I

I

L

L

D
|
D

E

E

I
|
I

Q

Q

A

T

G

G

F

M

G

G

R

R

T

T

G

G

R

K

W

R

F

F

A

A

H

F

H

E

A

H

A

F

D

G

I

I

T

V

P

P

D

D

I

I

I

L

T

T

L

L

A

A

K
|
K

G

A

L

L

A

G

G

G

I

V

P

P

M

L

G

G

A

V

T

A

I

V

G

M

V

R

G

E

D

E

W

V

G

A

N

R

V

S

F

M

Q

P

A

K

G

G

D

G

H

H

G

G

S

T

T
|
T

F

F

G

G

N

N

P

P

I

L

A

A

G

M

A

A

A

G

L

V

A

A

V

A

I

I

D

R

I

Y

I

L

E

E

R

R

E

T

D

R

L

L

F

W

D

E

N

R

V

A

N

A

T

E

L

L

G

G

R

P

K

W

L

F

E

M

N

E

R

K

L

L

H

R

-

A

-

I

N

P

H

S

P

P

A

K

V

I

I

R

A

E

V

V

R

R

G

G

E

M

A

G

L

L

W

M

R

V

G

G

I

L

V

E

L

L

D

K

T

E

D

K

I

A

A

A

A

P

D

Y

V

I

C

A

G

R

Q

L

L

E

R

K

N

E

A

H

G

R

I

V

L

L

A

A

N

L

P

Q

V

A

R

G

P

P

N

T

V

V

I

I

R
|
R

I

F

A

L

P

P

L

L

S

V

I

I

T

E

A

K

A

E

E

D

L

L

E

E

Q

R

F

V

T

V

S

E

A

A

L

V

T

R

D

A

V

V

L

L

active site: S13 (≠ T20), F132 (= F139), D217 (= D224), K246 (= K253), T275 (= T282), R363 (= R368)
binding pyridoxal-5'-phosphate: G105 (= G109), T106 (= T110), F132 (= F139), S133 (≠ H140), E184 (= E191), D217 (= D224), I219 (= I226), K246 (= K253)

O66442 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Aquifex aeolicus (strain VF5)
38% identity, 94% coverage: 17:391/401 of query aligns to 4:376/376 of O66442

query
sites
O66442

L

L

M

M

D

N

T

N

Y

Y

G

A

Q

R

P

L

P

P

V

V

A

K

L

F

A

V

S

R

G

G

N

K

G

G

R

V

H

Y

V

L

T

Y

D

D

V

E

D

E

G

G

R

K

T

E

Y

Y

L

L

D

D

M

F

I

V

G

S

G

G

I

I

A

G

V

V

S

N

T

S

V

L

G

G

H

H

R

A

H

Y

P

P

D

K

Y

L

V

T

E

E

A

A

V

L

V

K

R

E

Q

Q

T

V

A

E

T

K

I

L

A

L

H

H

T

V

S

S

N

N

L

L

F

Y

F

E

H

N

P

P

T

W

E

Q

V

E

E

E

L

L

A

A

E

H

T

K

L

L

V

V

K

K

L

H

T

F

G

W

A

T

D

E

G

G

R

K

V

V

F

F

S

A

N

N

S

S

G
|
G

T
|
T

E

E

A

S

N

V

E

E

A

A

L

I

K

K

L

L

A

A

L

R

K

K

A

Y

G

W

K

R

P

D

H

K

G

G

K

K

T

N

R

K

-

W

-

K

I

F

V

I

A

S

A

F

E

E

N

N

S

S

F

F

H

H

G

G

R

R

T

T

L

Y

G

G

T

S

L

L

A

S

V

A

T

T

G

G

K

Q

A

P

S

K

I

F

R

H

E

K

P

G

F

F

A

E

P

P

F

L

G

V

I

P

E

G

A

F

T

S

F

Y

V

A

P

K

Y

L

G

N

D

D

T

I

A

D

A

S

L

V

T

Y

A

K

A

L

V

L

D

D

D

E

T

E

V

T

A

A

A

G

V

I

I

I

L

I

E

E

P

V

T

I

Q

Q

G

G

E

E

A

G

G

G

V

V

V

N

P

E

A

A

S

S

T

E

D

D

F

F

L

L

H

S

T

K

A

L

R

Q

A

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L

I

T

C

H

K

E

E

H

K

G

D

S

V

A

L

L

L

I

I

L

I

D

D

E

E

I

V

Q

Q

A

T

G

G

F

I

G

G

R

R

T

T

G

G

R

E

W

F

F

Y

A

A

H

Y

H

Q

A

H

A

F

D

N

I

L

T

K

P

P

D

D

I

V

I

I

T

A

L

L

A

A

K
|
K

G

G

L

L

A

G

G

G

I

V

P

P

M

I

G

G

A

A

T

I

I

L

G

A

V

R

G

E

D

E

W

V

G

A

N

Q

V

S

F

F

Q

T

A

P

G

G

D

S

H

H

G

G

S

S

T
|
T

F

F

G

G

N

N

P

P

I

L

A

A

G

C

A

R

A

A

A

G

L

T

A

V

V

V

I

V

D

D

I

E

I

V

E

E

R

K

E

-

D

-

L

L

F

L

D

P

N

H

V

V

N

R

T

E

L

V

G

G

R

N

K

Y

L

F

E

K

N

E

R

K

L

L

H

-

N

K

H

E

P

L

A

G

V

K

I

G

A

K

V

V

R

K

G

G

E

R

A

G

L

L

W

M

R

L

G

G

I

L

V

E

L

L

D

E

T

R

D

E

I

C

A

K

A

D

D

Y

V

V

C

L

G

K

Q

A

L

L

R

E

N

K

A

-

G

G

I

L

L

L

A

I

N

N

P

C

V

T

R

A

P

G

N

K

V

V

I

L

R

R

I

F

A

L

P

P

L

L

S

I

I

I

T

Q

A

K

A

E

E

H

L

I

E

D

Q

R

F

A

T

I

S

S

A

V

L

L

T

R

D

E

V

I

L

L

GT 96:97 (= GT 109:110) binding pyridoxal 5'-phosphate
K242 (= K253) modified: N6-(pyridoxal phosphate)lysine
T271 (= T282) binding pyridoxal 5'-phosphate

2eh6A Crystal structure of acetylornithine aminotransferase from aquifex aeolicus vf5
38% identity, 94% coverage: 17:391/401 of query aligns to 3:375/375 of 2eh6A

query
sites
2eh6A

L

L

M

M

D

N

T

N

Y

Y

G

A

Q

R

P

L

P

P

V

V

A

K

L

F

A

V

S

R

G

G

N

K

G

G

R

V

H

Y

V

L

T

Y

D

D

V

E

D

E

G

G

R

K

T

E

Y

Y

L

L

D

D

M

F

I

V

G

S

G

G

I

I

A

G

V

V

S

N

T

S

V

L

G

G

H

H

R

A

H

Y

P

P

D

K

Y

L

V

T

E

E

A

A

V

L

V

K

R

E

Q

Q

T

V

A

E

T

K

I

L

A

L

H

H

T

V

S

S

N

N

L

L

F

Y

F

E

H

N

P

P

T

W

E

Q

V

E

E

E

L

L

A

A

E

H

T

K

L

L

V

V

K

K

L

H

T

F

G

W

A

T

D

E

G

G

R

K

V

V

F

F

S

A

N

N

S

S

G
|
G

T
|
T

E

E

A

S

N

V

E

E

A

A

L

I

K

K

L

L

A

A

L

R

K

K

A

Y

G

W

K

R

P

D

H

K

G

G

K

K

T

N

R

K

-

W

-

K

I

F

V

I

A

S

A

F

E

E

N

N

S

S

F
|
F

H
|
H

G

G

R

R

T

T

L

Y

G

G

T

S

L

L

A

S

V

A

T

T

G

G

K

Q

A

P

S

K

I

F

R

H

E

K

P

G

F

F

A

E

P

P

F

L

G

V

I

P

E

G

A

F

T

S

F

Y

V

A

P

K

Y

L

G

N

D

D

T

I

A

D

A

S

L

V

T

Y

A

K

A

L

V

L

D

D

D

E

T

E

V

T

A

A

A

G

V

I

I

I

L

I

E
|
E

P

V

T

I

Q

Q

G

G

E

E

A

G

G

G

V

V

V

N

P

E

A

A

S

S

T

E

D

D

F

F

L

L

H

S

T

K

A

L

R

Q

A

E

L

I

T

C

H

K

E

E

H

K

G

D

S

V

A

L

L

L

I

I

L

I

D
|
D

E

E

I
x
V

Q
|
Q

A

T

G

G

F

I

G

G

R

R

T

T

G

G

R

E

W

F

F

Y

A

A

H

Y

H

Q

A

H

A

F

D

N

I

L

T

K

P

P

D

D

I

V

I

I

T

A

L

L

A

A

K
|
K

G

G

L

L

A

G

G

G

I

V

P

P

M

I

G

G

A

A

T

I

I

L

G

A

V

R

G

E

D

E

W

V

G

A

N

Q

V

S

F

F

Q

T

A

P

G

G

D

S

H

H

G

G

S

S

T
|
T

F

F

G

G

N

N

P

P

I

L

A

A

G

C

A

R

A

A

A

G

L

T

A

V

V

V

I

V

D

D

I

E

I

V

E

E

R

K

E

-

D

-

L

L

F

L

D

P

N

H

V

V

N

R

T

E

L

V

G

G

R

N

K

Y

L

F

E

K

N

E

R

K

L

L

H

-

N

K

H

E

P

L

A

G

V

K

I

G

A

K

V

V

R

K

G

G

E

R

A

G

L

L

W

M

R

L

G

G

I

L

V

E

L

L

D

E

T

R

D

E

I

C

A

K

A

D

D

Y

V

V

C

L

G

K

Q

A

L

L

R

E

N

K

A

-

G

G

I

L

L

L

A

I

N

N

P

C

V

T

R

A

P

G

N

K

V

V

I

L

R
|
R

I

F

A

L

P

P

L

L

S

I

I

I

T

Q

A

K

A

E

E

H

L

I

E

D

Q

R

F

A

T

I

S

S

A

V

L

L

T

R

D

E

V

I

L

L

active site: F127 (= F139), E179 (= E191), D212 (= D224), Q215 (= Q227), K241 (= K253), T270 (= T282), R352 (= R368)
binding pyridoxal-5'-phosphate: G95 (= G109), T96 (= T110), F127 (= F139), H128 (= H140), E179 (= E191), D212 (= D224), V214 (≠ I226), K241 (= K253)

4addA Structural and functional study of succinyl-ornithine transaminase from e. Coli (see paper)
39% identity, 94% coverage: 10:387/401 of query aligns to 5:395/400 of 4addA

query
sites
4addA

Q

R

N

E

R

N

Y

F

A

D

D

E

A

W

L

M

M

I

D

P

T

V

Y
|
Y

G

A

Q

P

P

A

P

P

V

F

A

I

L

P

A

V

S

R

G

G

N

E

G

G

R

S

H

R

V

L

T

W

D

D

V

Q

D

Q

G

G

R

K

T

E

Y

Y

L

I

D

D

M

F

I

A

G

G

I

I

A

A

V

V

S

N

T

A

V

L

G

G

H

H

R

A

H

H

P

P

D

E

Y

L

V

R

E

E

A

A

V

L

V

N

R

E

Q

Q

T

A

A

S

T

K

I

F

A

W

H

H

T

T

S

G

N

N

L

G

F

Y

F

T

H

N

P

E

T

P

E

V

V

L

E

R

L

L

A

A

E

K

T

K

L

L

V

I

K

D

L

A

T

T

G

F

A

A

D

D

G

-

R

R

V

V

F

F

S

C

N

N

S

S

G
|
G

T
x
A

E

E

A

A

N

N

E

E

A

A

L

L

K

K

L

L

A

A

L

R

K

K

-

F

A

A

G

H

K

D

P

R

H

Y

G

G

-

S

-

H

K

K

T

S

R

G

I

I

V

V

A

A

A

F

E

K

N

N

S

A

F
|
F

H
|
H

G

G

R
|
R

T

T

L

L

G

F

T

T

L

V

A

S

V

A

T

G

G

G

K

Q

A

P

S

A

I

Y

R

S

E

Q

P

D

F

F

A

A

P

P

F

L

G

P

I

A

E

D

A

I

T

R

F

H

V

A

P

A

Y

Y

G

N

D

D

T

I

A

N

A

S

L

A

T

S

A

A

A

L

V

I

D

D

T

S

V

T

A

C

A

A

V

V

I

I

L

V

E
|
E

P

P

T

I

Q

Q

G

G

E

E

A

G

G

G

V

V

P

P

A

A

S

S

T

N

D

A

F

F

L

L

H

Q

T

G

A

L

R

R

A

E

L

L

T

C

H

N

E

R

H

H

G

N

S

A

A

L

L

L

I

I

L

F

D
|
D

E

E

I
x
V

Q
|
Q

A

T

G

G

F

V

G

G

R

R

T

T

G

G

R

E

W

L

F

Y

A

A

H

Y

H

M

A

H

A

Y

D

G

I

V

T

T

P

P

D

D

I

L

T

T

L

T

A

A

K
|
K

G

A

L

L

A

G

G

G

I

F

P

P

M

V

G

G

A

A

T

L

I

L

G

A

V

T

G

E

D

E

W

C

G

A

N

R

V

V

F

M

Q

T

A

V

G

G

D

T

H

H

G

G

S

T

T
|
T

F

Y

G

G

N

N

P

P

I

L

A

A

G

S

A

A

A

V

A

A

L

G

A

K

V

V

I

L

D

E

I

L

I

I

E

N

R

T

E

P

D

E

L

M

F

L

D

N

N

G

V

V

N

K

T

Q

L

R

G

H

R

D

K

W

L

F

E

V

N

E

R

R

L

L

-

N

-

T

-

I

-

N

H

H

N

R

H

Y

P

G

A

L

V

F

I

S

A

E

V

V

R

R

G

G

E

L

A

G

L

L

W

L

R

I

G

G

I

C

V

V

L

L

D

N

T

A

D

D

I

Y

A

A

A

G

-

Q

-

A

-

K

D

Q

V

I

C

S

G

Q

Q

E

L

A

R

A

N

K

A

A

G

G

I

V

L

M

A

V

N

L

P

I

V

A

R

G

P

G

N

N

V

V

I

V

R
|
R

I

F

A

A

P

P

P

A

L

L

S

N

I

V

T

S

A

E

E

E

-

V

-

T

-

G

L

L

E

D

Q

R

F

F

T

A

S

A

A

A

L

C

active site: F136 (= F139), E188 (= E191), D221 (= D224), Q224 (= Q227), K250 (= K253), T279 (= T282), R372 (= R368)
binding pyridoxal-5'-phosphate: G103 (= G109), A104 (≠ T110), F136 (= F139), H137 (= H140), D221 (= D224), V223 (≠ I226), K250 (= K253)
binding n~2~-(3-carboxypropanoyl)-l-ornithine: Y16 (= Y21), F136 (= F139), R139 (= R142)

4adbB Structural and functional study of succinyl-ornithine transaminase from e. Coli (see paper)
38% identity, 96% coverage: 10:393/401 of query aligns to 5:401/401 of 4adbB

query
sites
4adbB

Q

R

N

E

R

N

Y

F

A

D

D

E

A

W

L

M

M

I

D

P

T

V

Y

Y

G

A

Q

P

P

A

P

P

V

F

A

I

L

P

A

V

S

R

G

G

N

E

G

G

R

S

H

R

V

L

T

W

D

D

V

Q

D

Q

G

G

R

K

T

E

Y

Y

L

I

D

D

M

F

I

A

G

G

I

I

A

A

V

V

S

N

T

A

V

L

G

G

H

H

R

A

H

H

P

P

D

E

Y

L

V

R

E

E

A

A

V

L

V

N

R

E

Q

Q

T

A

A

S

T

K

I

F

A

W

H

H

T

T

S

G

N

N

L

G

F

Y

F

T

H

N

P

E

T

P

E

V

V

L

E

R

L

L

A

A

E

K

T

K

L

L

V

I

K

D

L

A

T

T

G

F

A

A

D

D

G

-

R

R

V

V

F

F

S

C

N

N

S
|
S

G
|
G

T
x
A

E

E

A

A

N

N

E

E

A

A

L

L

K

K

L

L

A

A

L

R

K

K

-

F

A

A

G

H

K

D

P

R

H

Y

G

G

-

S

-

H

K

K

T

S

R

G

I

I

V

V

A

A

A

F

E

K

N

N

S

A

F
|
F

H
|
H

G

G

R

R

T

T

L

L

G

F

T

T

L

V

A

S

V

A

T

G

G

G

K

Q

A

P

S

A

I

Y

R

S

E

Q

P

D

F

F

A

A

P

P

F

L

G

P

I

A

E

D

A

I

T

R

F

H

V

A

P

A

Y

Y

G

N

D

D

T

I

A

N

A

S

L

A

T

S

A

A

A

L

V

I

D

D

T

S

V

T

A

C

A

A

V

V

I

I

L

V

E
|
E

P

P

T

I

Q

Q

G

G

E

E

A

G

G

G

V

V

P

P

A

A

S

S

T

N

D

A

F

F

L

L

H

Q

T

G

A

L

R

R

A

E

L

L

T

C

H

N

E

R

H

H

G

N

S

A

A

L

L

L

I

I

L

F

D
|
D

E

E

I
x
V

Q
|
Q

A

T

G

G

F

V

G

G

R

R

T

T

G

G

R

E

W

L

F

Y

A

A

H

Y

H

M

A

H

A

Y

D

G

I

V

T

T

P

P

D

D

I

L

T

T

L

T

A

A

K
|
K

G

A

L

L

A

G

G

G

I

F

P

P

M

V

G

G

A

A

T

L

I

L

G

A

V

T

G

E

D

E

W

C

G

A

N

R

V

V

F

M

Q

T

A

V

G

G

D

T

H

H

G

G

S

T

T
|
T

F

Y

G

G

N

N

P

P

I

L

A

A

G

S

A

A

A

V

A

A

L

G

A

K

V

V

I

L

D

E

I

L

I

I

E

N

R

T

E

P

D

E

L

M

F

L

D

N

N

G

V

V

N

K

T

Q

L

R

G

H

R

D

K

W

L

F

E

V

N

E

R

R

L

L

-

N

-

T

-

I

-

N

H

H

N

R

H

Y

P

G

A

L

V

F

I

S

A

E

V

V

R

R

G

G

E

L

A

G

L

L

W

L

R

I

G

G

I

C

V

V

L

L

D

N

T

A

D

D

I

Y

A

A

A

G

-

Q

-

A

-

K

D

Q

V

I

C

S

G

Q

Q

E

L

A

R

A

N

K

A

A

G

G

I

V

L

M

A

V

N

L

P

I

V

A

R

G

P

G

N

N

V

V

I

V

R
|
R

I

F

A

A

P

P

P

A

L

L

S

N

I

V

T

S

A

E

E

E

-

V

-

T

-

G

L

L

E

D

Q

R

F

F

T

A

S

A

A

A

L

C

T

E

D

H

V

F

L

V

D

S

Q

R

active site: F136 (= F139), E188 (= E191), D221 (= D224), Q224 (= Q227), K250 (= K253), T279 (= T282), R372 (= R368)
binding pyridoxal-5'-phosphate: S102 (= S108), G103 (= G109), A104 (≠ T110), F136 (= F139), H137 (= H140), D221 (= D224), V223 (≠ I226), Q224 (= Q227), K250 (= K253)

P40732 Acetylornithine/succinyldiaminopimelate aminotransferase; ACOAT; DapATase; Succinyldiaminopimelate transferase; EC 2.6.1.11; EC 2.6.1.17 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
36% identity, 98% coverage: 1:391/401 of query aligns to 1:404/405 of P40732

query
sites
P40732

M

M

T

A

T

T

D

E

S

Q

T

T

T

A

A

I

L

T

Q

R

N

A

R

T

Y

F

A

D

D

E

A

V

L

I

M

L

D

P

T

V

Y

Y

G

A

Q

P

P

A

P

D

V

F

A

I

L

P

A

V

S

K

G

G

N

K

G

G

R

S

H

R

V

V

T

W

D

D

V

Q

D

Q

G

G

R

K

T

E

Y

Y

L

I

D

D

M

F

I

A

G

G

I

I

A

A

V

V

S

T

T

A

V

L

G

G

H

H

R

C

H

H

P

P

D

A

Y

L

V

V

E

E

A

A

V

L

V

K

R

S

Q

Q

T

G

A

E

T

T

I

L

A

W

H

H

T

T

S

S

N

N

L

V

F

F

F

T

H

N

P

E

T

P

E

A

V

L

E

R

L

L

A

G

E

R

T

K

L

L

V

I

K

D

L

A

T

T

G

F

A

A

D

E

G

-

R

R

V

V

F

L

F

F

S

M

N

N

S

S

G
|
G

T
|
T

E

E

A

A

N

N

E

E

A

T

A

A

L

F

K

K

L

L

A

A

L

R

K

H

-

Y

A

A

G

C

K

V

P

R

H

H

G

S

-

P

-

F

K

K

T

T

R

K

I

I

V

I

A

A

A

F

E

H

N

N

S

A

F

F

H

H

G

G

R

R

T

S

L

L

G

F

T

T

L

V

A

S

V

V

T

G

G

G

K

Q

A

P

S

K

I

Y

R

S

E

D

P

G

F

F

A

G

P

P

F

K

G

P

I

A

E

D

A

I

T

I

F

H

V

V

P

P

Y

F

G

N

D

D

T

L

A

H

A

A

L

V

T

K

A

A

A

V

V

M

D

D

T

H

V

T

A

C

A

A

V

V

I

V

L

V

E

E

P

P

T

I

Q

Q

G

G

E

E

A

G

G

G

V

V

V

Q

P

A

A

A

S

T

T

P

D

E

F

F

L

L

H

K

T

G

A

L

R

R

A

D

L

L

T

C

H

D

E

E

H

H

G

Q

S

A

A

L

L

L

I

V

L

F

D

D

E

E

I

V

Q

Q

A

C

G

G

F

M

G

G

R

R

T

T

G

G

R

D

W

L

F

F

A

A

H

Y

H

M

A

H

A

Y

D

G

I

V

T

T

P

P

D

D

I

I

I

L

T

T

L

S

A

A

K
|
K

G

A

L

L

A

G

G

G

I

F

P

P

M

V

G

S

A

A

T

M

I

L

G

T

V

T

G

Q

D

E

W

I

G

A

N

S

V

A

F

F

Q

H

A

V

G

G

D

S

H

H

G

G

S

S

T
|
T

F

Y

G

G

N

N

P

P

I

L

A

A

G

C

A

A

A

V

A

A

L

G

A

A

V

A

I

F

D

D

I

I

E

N

R

T

E

P

D

E

L

V

F

L

D

Q

N

G

V

I

N

H

T

T

L

K

G

R

R

Q

K

Q

L

F

E

V

N

Q

R

H

L

L

H

Q

N

A

H

I

P

D

A

E

V

Q

I

F

A

D

V

I

R

F

G

S

E

D

A

I

L

R

W

G

R

M

G

G

I

L

V

L

L

I

D

G

T

A

D

E

I

L

A

K

A

P

D

K

V

Y

C

K

G

G

Q

R

L

A

R

R

N

D

-

F

-

L

-

Y

-

A

-

G

-

A

-

E

A

A

G

G

I

V

L

M

A

V

N

L

P

N

V

A

R

G

P

A

N

D

V

V

I

M

R

R

I

F

A

A

P

P

P

S

L

L

S

V

I

V

T

E

A

E

A

A

E

D

-

I

-

H

-

E

-

G

L

M

E

Q

Q

R

F

F

T

A

S

Q

A

A

L

V

T

G

D

K

V

V

L

V

GT 108:109 (= GT 109:110) binding pyridoxal 5'-phosphate
K255 (= K253) modified: N6-(pyridoxal phosphate)lysine
T284 (= T282) binding pyridoxal 5'-phosphate

4jevB N-acetylornithine aminotransferase from s. Typhimurium complexed with gabaculine
36% identity, 95% coverage: 13:391/401 of query aligns to 8:399/402 of 4jevB

query
sites
4jevB

Y

F

A

D

D

E

A

V

L

I

M

L

D

P

T

V

Y

Y

G

A

Q

P

P

A

P

D

V

F

A

I

L

P

A

V

S

K

G

G

N

K

G

G

R

S

H

R

V

V

T

W

D

D

V

Q

D

Q

G

G

R

K

T

E

Y

Y

L

I

D

D

M

F

I

A

G

G

I
|
I

A

A

V

V

S

T

T

A

V

L

G

G

H

H

R

C

H

H

P

P

D

A

Y

L

V

V

E

E

A

A

V

L

V

K

R

S

Q

Q

T

G

A

E

T

T

I

L

A

W

H

H

T

T

S

S

N

N

L

V

F

F

F

T

H

N

P

E

T

P

E

A

V

L

E

R

L

L

A

G

E

R

T

K

L

L

V

I

K

D

L

A

T

T

G

F

A

A

D

E

G

-

R

R

V

V

F

L

F

F

S

M

N

N

S
|
S

G
|
G

T
|
T

E

E

A

A

N

N

E

E

A

T

A

A

L

F

K

K

L

L

A

A

L

R

K

H

-

Y

A

A

G

C

K

V

P

R

H

H

G

S

-

P

-

F

K

K

T

T

R

K

I

I

V

I

A

A

A

F

E

H

N

N

S

A

F
|
F

H
|
H

G

G

R

R

T

S

L

L

G

F

T

T

L

V

A

S

V

V

T

G

G

G

K

Q

A

P

S

K

I

Y

R

S

E

D

P

G

F

F

A

G

P

P

F

K

G

P

I

A

E

D

A

I

T

I

F

H

V

V

P

P

Y

F

G

N

D

D

T

L

A

H

A

A

L

V

T

K

A

A

A

V

V

M

D

D

T

H

V

T

A

C

A

A

V

V

I

V

L

V

E
|
E

P

P

T

I

Q

Q

G

G

E
|
E

A

G

G

G

V

V

V

Q

P

A

A

A

S

T

T

P

D

E

F

F

L

L

H

K

T

G

A

L

R

R

A

D

L

L

T

C

H

D

E

E

H

H

G

Q

S

A

A

L

L

L

I

V

L

F

D
|
D

E

E

I
x
V

Q
|
Q

A

C

G

G

F

M

G

G

R

R

T

T

G

G

R

D

W

L

F

F

A

A

H

Y

H

M

A

H

A

Y

D

G

I

V

T

T

P

P

D

D

I

I

I

L

T

T

L

S

A

A

K
|
K

G

A

L

L

A

G

G

G

I

F

P

P

M

V

G

S

A

A

T

M

I

L

G

T

V

T

G

Q

D

E

W

I

G

A

N

S

V

A

F

F

Q

H

A

V

G

G

D

S

H

H

G

G

S

S

T
|
T

F

Y

G

G

N

N

P

P

I

L

A

A

G

C

A

A

A

V

A

A

L

G

A

A

V

T

I

F

D

D

I

I

E

N

R

T

E

P

D

E

L

V

F

L

D

Q

N

G

V

I

N

H

T

T

L

K

G

R

R

Q

K

Q

L

F

E

V

N

Q

R

H

L

L

H

Q

N

A

H

I

P

D

A

E

V

Q

I

F

A

D

V

I

R

F

G

S

E

D

A

I

L

R

W

G

R

M

G

G

I

L

V

L

L

I

D

G

T

A

D

E

I

L

A

K

A

P

D

K

V

Y

C

K

G

G

Q

R

L

A

R

R

N

D

-

F

-

L

-

Y

-

A

-

G

-

A

-

E

A

A

G

G

I

V

L

M

A

V

N

L

P

N

V

A

R

G

P

A

N

D

V

V

I

M

R
|
R

I

F

A

A

P

P

P

S

L

L

S

V

I

V

T

E

A

E

A

A

E

D

-

I

-

H

-

E

-

G

L

M

E

Q

Q

R

F

F

T

A

S

Q

A

A

L

V

T

G

D

K

V

V

L

V

active site: F136 (= F139), E188 (= E191), D221 (= D224), Q224 (= Q227), K250 (= K253), T279 (= T282), R372 (= R368)
binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: I46 (= I51), S102 (= S108), G103 (= G109), T104 (= T110), F136 (= F139), H137 (= H140), E188 (= E191), E193 (= E196), D221 (= D224), V223 (≠ I226), Q224 (= Q227), K250 (= K253), R372 (= R368)

2byjA Ornithine aminotransferase mutant y85i (see paper)
38% identity, 95% coverage: 2:382/401 of query aligns to 4:392/404 of 2byjA

query
sites
2byjA

T

T

T

S

D

D

S

D

T

I

T

F

A

E

L

R

Q

E

N

Y

R

K

Y

Y

A

G

D

-

A

-

L

-

M

A

D

H

T

N

Y

Y

G

H

Q

P

P

L

P

P

V

V

A

A

L

L

A

E

S

R

G

G

N

K

G

G

R

I

H

Y

V

L

T

W

D

D

V

V

D

E

G

G

R

R

T

K

Y

Y

L

F

D

D

M

F

I

L

G

S

I

I

A

S

V

A

S

V

T

N

V

Q

G

G

H

H

R

C

H

H

P

P

D

K

Y

I

V

V

E

N

A

A

V

L

V

K

R

S

Q

Q

T

V

A

D

T

K

I

L

A

T

H

L

T

T

S

S

N

R

L

A

F

F

F

Y

H

N

P

N

T

V

E

L

V

G

E

E

L

Y

A

E

E

E

T

Y

L

I

V

T

K

K

L

L

T

F

G

N

A

Y

D

H

G

-

R

K

V

V

F

L

F

P

S

M

N

N

S

T

G
|
G

T
x
V

E

E

A

A

N

G

E

E

A

T

A

A

L

C

K

K

L

L

A

A

L

R

K

K

A

W

G

G

K

Y

-

T

-

V

-

K

-

G

-

I

P

Q

H

K

G

Y

K

K

T

A

R

K

I

I

V

V

A

F

A

A

E

A

N

G

S

N

F
|
F

H
x
W

G

G

R

R

T

T

L

L

G

S

T

A

L

I

A

S

V

S

T

S

G

T

K

D

A

P

S

T

I

S

R

Y

E

D

P

G

F

F

A

G

P

P

F

F

G

M

I

P

E

G

A

F

T

D

F

I

V

I

P

P

Y

Y

G

N

D

D

T

L

A

P

A

A

L

L

T

E

A

R

A

A

V

L

D

Q

D

D

-

P

T

N

V

V

A

A

V

F

I

M

L

V

E
|
E

P

P

T

I

Q

Q

G

G

E

E

A

A

G

G

V

V

P

V

A

P

S

D

T

P

D

G

F

Y

L

L

H

M

T

G

A

V

R

R

A

E

L

L

T

C

H

T

E

R

H

H

G

Q

S

V

A

L

L

F

I

I

L

A

D
|
D

E

E

I
|
I

Q
|
Q

A

T

G

G

F

L

G

A

R

R

T

T

G

G

R

R

W

W

F

L

A

A

H

V

H

D

A

Y

A

E

D

N

I

V

T

R

P

P

D

D

I

I

I

V

T

L

L

L

A

G

K
|
K

G

A

L

L

A

S

G

G

I

L

-

Y

P

P

M

V

G

S

A

A

T

V

I

L

G

C

V

D

G

D

D

D

W

I

G

M

N

L

V

T

F

I

Q

K

A

P

G

G

D

E

H

H

G

G

S

S

T
|
T

F

Y

G

G

N

N

P

P

I

L

A

G

G

C

A

R

A

V

A

A

L

I

A

A

V

A

I

L

D

E

I

V

I

L

E

E

R

E

E

E

D

N

L

L

F

A

D

E

N

N

V

A

N

D

T

K

L

L

G

G

R

I

K

I

L

L

E

R

N

N

R

E

L

L

H

M

N

K

H

L

P

P

A

S

-

D

-

V

V

V

I

T

A

A

V

V

R

R

G

G

E

K

A

G

L

L

W

L

R

N

G

A

I

I

V

V

L

I

-

K

-

E

-

T

-

K

D

D

T

W

D

D

I

-

A

A

A

W

D

K

V

V

C

C

G

L

Q

R

L

L

R

R

N

D

A

N

G

G

I

L

L

L

A

A

N

K

P

P

V

T

R

H

P

G

N

D

V

I

I

I

R
|
R

I

F

A

A

P

P

L

L

S

V

I

I

T

K

A

E

A

D

E

E

L

L

E

R

Q

E

active site: F142 (= F139), E195 (= E191), D228 (= D224), Q231 (= Q227), K257 (= K253), T287 (= T282), R378 (= R368)
binding pyridoxal-5'-phosphate: G107 (= G109), V108 (≠ T110), F142 (= F139), W143 (≠ H140), D228 (= D224), I230 (= I226), Q231 (= Q227), K257 (= K253)

Query Sequence

>WP_025274361.1 NCBI__GCF_000527155.1:WP_025274361.1
MTTDSTTALQNRYADALMDTYGQPPVALASGNGRHVTDVDGRTYLDMIGGIAVSTVGHRH
PDYVEAVVRQTATIAHTSNLFFHPTEVELAETLVKLTGADGRVFFSNSGTEANEAALKLA
LKAGKPHGKTRIVAAENSFHGRTLGTLAVTGKASIREPFAPFGIEATFVPYGDTAALTAA
VDDTVAAVILEPTQGEAGVVPASTDFLHTARALTHEHGSALILDEIQAGFGRTGRWFAHH
AADITPDIITLAKGLAGGIPMGATIGVGDWGNVFQAGDHGSTFGGNPIAGAAALAVIDII
EREDLFDNVNTLGRKLENRLHNHPAVIAVRGEALWRGIVLDTDIAADVCGQLRNAGILAN
PVRPNVIRIAPPLSITAAELEQFTSALTDVLDQYPAQGKTP

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory