SitesBLAST
Comparing WP_025274425.1 NCBI__GCF_000527155.1:WP_025274425.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
5ovkA Crystal structure maba bound to NADPH from m. Smegmatis (see paper)
62% identity, 100% coverage: 1:234/234 of query aligns to 9:242/242 of 5ovkA
5ovlA Crystal structure of maba bound to NADP+ from m. Smegmatis (see paper)
62% identity, 100% coverage: 1:234/234 of query aligns to 8:241/241 of 5ovlA
- active site: G20 (= G13), S134 (= S127), Y147 (= Y140), L154 (= L147)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G16 (= G9), N18 (= N11), R19 (= R12), G20 (= G13), I21 (= I14), R41 (= R34), D55 (= D48), V56 (≠ I49), N82 (= N75), A83 (= A76), I85 (≠ V78), T105 (= T98), I132 (= I125), S134 (= S127), Y147 (= Y140), K151 (= K144), P177 (= P170), G178 (= G171), I180 (≠ V173)
P71534 3-oxoacyl-[acyl-carrier-protein] reductase MabA; 3-ketoacyl-acyl carrier protein reductase; Acetoacetyl-CoA reductase; Beta-ketoacyl-ACP reductase; Beta-ketoacyl-acyl carrier protein reductase; EC 1.1.1.100; EC 1.1.1.36 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
62% identity, 100% coverage: 1:234/234 of query aligns to 22:255/255 of P71534
- NRGI 32:35 (= NRGI 11:14) binding NADP(+)
- R55 (= R34) binding NADP(+)
- DV 69:70 (≠ DI 48:49) binding NADP(+)
- Y161 (= Y140) binding NADP(+)
- K165 (= K144) binding NADP(+)
- I194 (≠ V173) binding NADP(+)
- R205 (≠ D184) binding NADP(+)
P9WGT3 3-oxoacyl-[acyl-carrier-protein] reductase MabA; 3-ketoacyl reductase; 3-ketoacyl-acyl carrier protein reductase; Acetoacetyl-CoA reductase; Beta-ketoacyl-acyl carrier protein reductase; Beta-ketoacyl-ACP reductase; Mycolic acid biosynthesis A; EC 1.1.1.100; EC 1.1.1.36 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 7 papers)
61% identity, 100% coverage: 1:234/234 of query aligns to 14:247/247 of P9WGT3
- T21 (= T8) modified: Phosphothreonine; mutation to A: Slight decrease in phosphorylation by PknB. Lack of phosphorylation by PknB; when associated with A-114 and A-191.
- RGI 25:27 (= RGI 12:14) binding NADP(+)
- R47 (= R34) binding NADP(+)
- C60 (= C47) mutation to V: Displays a lower activity than the wild-type and a slightly decreased affinity for the cofactor. Retains 84% of activity; when associated with L-144. Totally inactive; when associated with A-139 and L-144.
- DV 61:62 (≠ DI 48:49) binding NADP(+)
- G90 (= G77) binding NADP(+)
- T114 (= T101) modified: Phosphothreonine; mutation to A: Slight decrease in phosphorylation by PknB. Lack of phosphorylation by PknB; when associated with A-21 and A-191.
- G139 (≠ S126) mutation to A: Complete protein inactivation and freezes the catalytic site into its closed form. Totally inactive; when associated with V-60 and L-144.
- S140 (= S127) mutation to A: Loss of activity. Can still bind NADPH.; mutation to T: Loss of activity. Impaired NADPH binding.
- S144 (≠ M131) mutation to L: Stabilizes the catalytic loop in its open active form. Retains 84% of activity; when associated with V-60. Totally inactive; when associated with V-60 and A-139.
- Y185 (≠ F172) mutation to L: 70% decrease in activity with acetoacetyl-CoA as substrate. Does not affect NADP binding.
- T191 (= T178) modified: Phosphothreonine; mutation to A: Retains 22% of wild-type reductase activity. Strong decrease in phosphorylation by PknB. Lack of phosphorylation by PknB; when associated with A-21 and A-114.; mutation to D: Phosphomimetic mutant that retains less than 10% of wild-type reductase activity. Impaired NADPH binding. Overproduction of the mutant leads to a significant inhibition of de novo biosynthesis of mycolic acids.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
1uznA Maba from mycobacterium tuberculosis (see paper)
61% identity, 100% coverage: 1:234/234 of query aligns to 6:239/239 of 1uznA
- active site: G18 (= G13), S132 (= S127), Y145 (= Y140), K149 (= K144)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G14 (= G9), N16 (= N11), R17 (= R12), I19 (= I14), R39 (= R34), D53 (= D48), V54 (≠ I49), A81 (= A76), G82 (= G77)
7emgB Carbonyl reductase variant 4 (r123c/l209p/f183y/v61k) from serratia marcescens complexed with NADP+ (see paper)
45% identity, 98% coverage: 3:232/234 of query aligns to 5:242/243 of 7emgB
P0AEK2 3-oxoacyl-[acyl-carrier-protein] reductase FabG; 3-ketoacyl-acyl carrier protein reductase; Beta-Ketoacyl-acyl carrier protein reductase; Beta-ketoacyl-ACP reductase; EC 1.1.1.100 from Escherichia coli (strain K12) (see 2 papers)
44% identity, 98% coverage: 3:232/234 of query aligns to 6:243/244 of P0AEK2
- GASR 12:15 (≠ GGNR 9:12) binding NADP(+)
- T37 (≠ V31) binding NADP(+)
- NV 59:60 (≠ DI 48:49) binding NADP(+)
- N86 (= N75) binding NADP(+)
- Y151 (= Y140) mutation to F: Defect in the affinity for NADPH.
- YAAAK 151:155 (≠ YAASK 140:144) binding NADP(+)
- A154 (≠ S143) mutation to T: Decreases in the thermolability of the reductase; when associated with K-233.
- K155 (= K144) mutation to A: Defect in the affinity for NADPH.
- I184 (≠ V173) binding NADP(+)
- E233 (≠ A222) mutation to K: Decreases in the thermolability of the reductase; when associated with T-154.
1q7bA The structure of betaketoacyl-[acp] reductase from e. Coli in complex with NADP+ (see paper)
44% identity, 98% coverage: 3:232/234 of query aligns to 5:242/243 of 1q7bA
- active site: G15 (= G13), E101 (≠ Q91), S137 (= S127), Q147 (= Q137), Y150 (= Y140), K154 (= K144)
- binding calcium ion: E232 (≠ A222), T233 (≠ I223)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G9), S13 (≠ N11), R14 (= R12), T36 (≠ V31), N58 (≠ D48), V59 (≠ I49), N85 (= N75), A86 (= A76), G87 (= G77), I88 (≠ V78), S137 (= S127), Y150 (= Y140), K154 (= K144), P180 (= P170), G181 (= G171), I183 (≠ V173)
6t77A Crystal structure of klebsiella pneumoniae fabg(NADPH-dependent) NADP- complex at 1.75 a resolution (see paper)
42% identity, 98% coverage: 3:232/234 of query aligns to 6:243/244 of 6t77A
- active site: G16 (= G13), S138 (= S127), Y151 (= Y140)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G9), S14 (≠ N11), R15 (= R12), T37 (≠ R34), L58 (≠ C47), N59 (≠ D48), V60 (≠ I49), A87 (= A76), G88 (= G77), I89 (≠ V78)
P0A2C9 3-oxoacyl-[acyl-carrier-protein] reductase FabG; 3-ketoacyl-acyl carrier protein reductase; Beta-Ketoacyl-acyl carrier protein reductase; Beta-ketoacyl-ACP reductase; EC 1.1.1.100 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
43% identity, 98% coverage: 3:232/234 of query aligns to 6:243/244 of P0A2C9
- M125 (= M114) mutation to I: Loss of the temperature-sensitive phenotype; when associated with T-223.
- A223 (≠ S212) mutation to T: Loss of the temperature-sensitive phenotype; when associated with I-125.
- S224 (≠ G213) mutation to F: Distorts the local conformation and prevent stacking around Phe-221. The S224F mutation would additionally disrupt the hydrogen bond formed between Ser-224 and Glu-226.
1q7cA The structure of betaketoacyl-[acp] reductase y151f mutant in complex with NADPH fragment (see paper)
44% identity, 98% coverage: 3:232/234 of query aligns to 5:242/243 of 1q7cA
- active site: G15 (= G13), S137 (= S127), Q147 (= Q137), F150 (≠ Y140), K154 (= K144)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (= G9), S13 (≠ N11), R14 (= R12), A35 (= A30), T36 (≠ V31), L57 (≠ C47), N58 (≠ D48), V59 (≠ I49), G87 (= G77), I88 (≠ V78)
3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
42% identity, 99% coverage: 1:232/234 of query aligns to 3:246/246 of 3osuA
6t62A Crystal structure of acinetobacter baumannii fabg in complex with NADPH at 1.8 a resolution (see paper)
43% identity, 98% coverage: 3:232/234 of query aligns to 6:243/244 of 6t62A
- active site: G16 (= G13), S138 (= S127), Y151 (= Y140)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G9), S14 (≠ N11), R15 (= R12), A36 (≠ H33), T37 (≠ R34), L58 (≠ C47), D59 (= D48), V60 (≠ I49), N86 (= N75), A87 (= A76), G88 (= G77), I89 (≠ V78), I136 (= I125), S137 (= S126), S138 (= S127), Y151 (= Y140), K155 (= K144), P181 (= P170), G182 (= G171), I184 (≠ V173), M188 (= M177)
6wprA Crystal structure of a putative 3-oxoacyl-acp reductase (fabg) with NADP(h) from acinetobacter baumannii (see paper)
43% identity, 98% coverage: 3:232/234 of query aligns to 6:243/244 of 6wprA
- active site: G16 (= G13), S138 (= S127), Y151 (= Y140)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G12 (= G9), S14 (≠ N11), R15 (= R12), T37 (≠ R34), L58 (≠ C47), D59 (= D48), V60 (≠ I49), N86 (= N75), A87 (= A76), G88 (= G77), I89 (≠ V78), I136 (= I125), Y151 (= Y140), K155 (= K144), P181 (= P170)
4ag3A Crystal structure of 3-ketoacyl-(acyl-carrier-protein) reductase (fabg) from pseudomonas aeruginosa in complex with NADPH at 1.8a resolution (see paper)
45% identity, 98% coverage: 3:232/234 of query aligns to 13:253/254 of 4ag3A
- active site: G23 (= G13), S148 (= S127), Y161 (= Y140), K165 (= K144)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G19 (= G9), S21 (≠ N11), R22 (= R12), G23 (= G13), I24 (= I14), T44 (vs. gap), L68 (≠ C47), D69 (= D48), V70 (≠ I49), N96 (= N75), A97 (= A76), I146 (= I125), S148 (= S127), Y161 (= Y140), K165 (= K144), P191 (= P170), G192 (= G171), F193 (= F172), I194 (≠ V173), T196 (= T175), M198 (= M177), T199 (= T178)
3op4A Crystal structure of putative 3-ketoacyl-(acyl-carrier-protein) reductase from vibrio cholerae o1 biovar eltor str. N16961 in complex with NADP+ (see paper)
42% identity, 98% coverage: 3:232/234 of query aligns to 9:246/247 of 3op4A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G9), S17 (≠ N11), R18 (= R12), I20 (= I14), T40 (≠ R34), N62 (≠ D48), V63 (≠ I49), N89 (= N75), A90 (= A76), I92 (≠ V78), V139 (≠ I125), S141 (= S127), Y154 (= Y140), K158 (= K144), P184 (= P170), G185 (= G171), I187 (≠ V173), T189 (= T175), M191 (= M177)
3sj7A Structure of beta-ketoacetyl-coa reductase (fabg) from staphylococcus aureus complex with NADPH (see paper)
42% identity, 99% coverage: 2:232/234 of query aligns to 1:239/239 of 3sj7A
- active site: G12 (= G13), S138 (= S127), Q148 (= Q137), Y151 (= Y140), K155 (= K144)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G9), S10 (≠ N11), R11 (= R12), I13 (= I14), N31 (≠ T32), Y32 (≠ H33), A33 (≠ R34), G34 (= G35), S35 (= S36), A58 (≠ C47), N59 (≠ D48), V60 (≠ I49), N86 (= N75), A87 (= A76), T109 (= T98), S138 (= S127), Y151 (= Y140), K155 (= K144), P181 (= P170), G182 (= G171)
4i08A Crystal structure of beta-ketoacyl-acyl carrier protein reductase (fabg) from vibrio cholerae in complex with NADPH (see paper)
41% identity, 98% coverage: 3:232/234 of query aligns to 9:242/243 of 4i08A
- active site: G19 (= G13), N113 (= N99), S141 (= S127), Q151 (= Q137), Y154 (= Y140), K158 (= K144)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G15 (= G9), S17 (≠ N11), R18 (= R12), I20 (= I14), T40 (≠ R34), N62 (≠ D48), V63 (≠ I49), N89 (= N75), A90 (= A76), G140 (≠ S126), S141 (= S127), Y154 (= Y140), K158 (= K144), P184 (= P170), G185 (= G171), T189 (= T175)
4bo4C Crystal structure of 3-oxoacyl-(acyl-carrier-protein) reductase (fabg) from pseudomonas aeruginosa in complex with n-(2-methoxyphenyl)-3,4- dihydro-2h-quinoline-1-carboxamide at 2.7a resolution (see paper)
45% identity, 98% coverage: 3:232/234 of query aligns to 19:254/255 of 4bo4C
4bnzA Crystal structure of 3-oxoacyl-(acyl-carrier-protein) reductase (fabg) from pseudomonas aeruginosa in complex with 1-methyl-n-phenylindole- 3-carboxamide at 2.5a resolution (see paper)
44% identity, 98% coverage: 3:232/234 of query aligns to 8:240/241 of 4bnzA
Query Sequence
>WP_025274425.1 NCBI__GCF_000527155.1:WP_025274425.1
MSRTVLVTGGNRGIGLAIARAFEKNGDRVAVTHRGSGAPEGLYGVQCDITDSDSVNAAFE
QIEADLGPVEVLVANAGVTDDTLLLRMSEEQFENVLNTNLTGAWRCAKRASSKMLRAKFG
RMIFISSVVGMLGNAGQTNYAASKAGLVGLARSITRELGSRNITANVIAPGFVETDMTAE
LSDDQRKAYLDTIPAKRLAQPEEIAATATWLSGNDAGYINGAIIPVDGGLGMGH
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory