SitesBLAST
Comparing WP_025329670.1 NCBI__GCF_000600005.1:WP_025329670.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
35% identity, 82% coverage: 54:309/312 of query aligns to 56:313/334 of 5aovA
- active site: L100 (≠ G98), R241 (= R234), D265 (= D258), E270 (= E263), H288 (= H284)
- binding glyoxylic acid: Y74 (≠ P72), A75 (= A73), V76 (≠ T74), G77 (= G75), R241 (= R234), H288 (= H284)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (≠ T74), T104 (≠ V102), F158 (≠ K155), G159 (= G156), R160 (≠ G157), I161 (= I158), S180 (≠ E177), R181 (≠ H178), A211 (≠ H204), V212 (≠ C205), P213 (= P206), T218 (= T211), I239 (≠ V232), A240 (≠ G233), R241 (= R234), H288 (= H284), G290 (≠ A286)
Sites not aligning to the query:
6biiA Crystal structure of pyrococcus yayanosii glyoxylate hydroxypyruvate reductase in complex with NADP and malonate (re-refinement of 5aow) (see paper)
34% identity, 81% coverage: 54:307/312 of query aligns to 55:310/332 of 6biiA
- active site: L99 (≠ G98), R240 (= R234), D264 (= D258), E269 (= E263), H287 (= H284)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V75 (≠ T74), T103 (≠ V102), G156 (= G154), F157 (≠ K155), G158 (= G156), R159 (≠ G157), I160 (= I158), A179 (≠ E177), R180 (≠ H178), S181 (≠ K179), K183 (≠ A181), V211 (≠ C205), P212 (= P206), E216 (≠ Q210), T217 (= T211), V238 (= V232), A239 (≠ G233), R240 (= R234), D264 (= D258), H287 (= H284), G289 (≠ A286)
1wwkA Crystal structure of phosphoglycerate dehydrogenase from pyrococcus horikoshii ot3
34% identity, 84% coverage: 35:295/312 of query aligns to 30:289/304 of 1wwkA
- active site: S96 (≠ G98), R230 (= R234), D254 (= D258), E259 (= E263), H278 (= H284)
- binding nicotinamide-adenine-dinucleotide: V100 (= V102), G146 (= G154), F147 (≠ K155), G148 (= G156), R149 (≠ G157), I150 (= I158), Y168 (vs. gap), D169 (vs. gap), P170 (vs. gap), V201 (≠ C205), P202 (= P206), T207 (= T211), T228 (≠ V232), S229 (≠ G233), D254 (= D258), H278 (= H284), G280 (≠ A286)
3kb6B Crystal structure of d-lactate dehydrogenase from aquifex aeolicus complexed with NAD and lactic acid (see paper)
31% identity, 78% coverage: 54:296/312 of query aligns to 53:306/334 of 3kb6B
- active site: S97 (≠ G98), R231 (= R234), D255 (= D258), E260 (= E263), H294 (= H284)
- binding lactic acid: S72 (≠ A73), V73 (≠ T74), G74 (= G75), Y96 (= Y97), R231 (= R234), H294 (= H284)
- binding nicotinamide-adenine-dinucleotide: V73 (≠ T74), Y96 (= Y97), V101 (= V102), G150 (= G156), R151 (≠ G157), I152 (= I158), D171 (≠ E177), V172 (≠ H178), P203 (= P206), T229 (≠ V232), A230 (≠ G233), R231 (= R234), H294 (= H284), A296 (= A286), Y297 (≠ W287)
Sites not aligning to the query:
5z20F The ternary structure of d-lactate dehydrogenase from pseudomonas aeruginosa with nadh and oxamate (see paper)
38% identity, 76% coverage: 67:304/312 of query aligns to 77:322/336 of 5z20F
- active site: S108 (≠ G98), R241 (= R234), D265 (= D258), E270 (= E263), H302 (= H284)
- binding 1,4-dihydronicotinamide adenine dinucleotide: Y107 (= Y97), G160 (= G156), Q161 (≠ G157), I162 (= I158), Y180 (≠ W175), D181 (≠ G176), P182 (≠ E177), C212 (= C205), P213 (= P206), T218 (= T211), T239 (≠ V232), G240 (= G233), R241 (= R234), H302 (= H284), A304 (= A286)
Q65CJ7 Hydroxyphenylpyruvate reductase; HPPR; EC 1.1.1.237 from Plectranthus scutellarioides (Coleus) (Solenostemon scutellarioides) (see paper)
32% identity, 80% coverage: 56:305/312 of query aligns to 58:300/313 of Q65CJ7
3bazA Structure of hydroxyphenylpyruvate reductase from coleus blumei in complex with NADP+ (see paper)
32% identity, 80% coverage: 56:305/312 of query aligns to 56:298/311 of 3bazA
- active site: L98 (≠ G98), R230 (= R234), A251 (= A255), D254 (= D258), E259 (= E263), H277 (= H284)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V74 (≠ T74), G149 (= G154), L150 (≠ K155), G151 (= G156), R152 (≠ G157), I153 (= I158), S172 (≠ G176), R173 (≠ E177), S174 (≠ H178), C201 (= C205), P202 (= P206), T207 (= T211), I228 (≠ V232), G229 (= G233), R230 (= R234), D254 (= D258), H277 (= H284), G279 (≠ A286)
6p2iA Acyclic imino acid reductase (bsp5) in complex with NADPH and d-arg (see paper)
31% identity, 98% coverage: 5:310/312 of query aligns to 2:300/307 of 6p2iA
- binding d-arginine: E51 (≠ N51), T73 (≠ A73), T74 (= T74), S75 (≠ G75), Y97 (= Y97), W277 (= W287)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: S98 (≠ G98), V102 (= V102), G149 (= G154), I150 (≠ K155), G151 (= G156), Q152 (≠ G157), I153 (= I158), N172 (≠ H178), K173 (= K179), S174 (≠ N180), R176 (≠ S182), H199 (= H204), I200 (≠ C205), P201 (= P206), T206 (= T211), T227 (≠ V232), C228 (≠ G233), W277 (= W287)
2dbqA Crystal structure of glyoxylate reductase (ph0597) from pyrococcus horikoshii ot3, complexed with NADP (i41) (see paper)
35% identity, 82% coverage: 54:309/312 of query aligns to 56:313/333 of 2dbqA
- active site: L100 (≠ G98), R241 (= R234), D265 (= D258), E270 (= E263), H288 (= H284)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (≠ T74), T104 (≠ V102), L158 (≠ K155), G159 (= G156), R160 (≠ G157), I161 (= I158), S180 (≠ E177), R181 (≠ H178), T182 (≠ K179), A211 (≠ H204), V212 (≠ C205), P213 (= P206), T218 (= T211), I239 (≠ V232), A240 (≠ G233), R241 (= R234), D265 (= D258), H288 (= H284), G290 (≠ A286)
O58320 Glyoxylate reductase; EC 1.1.1.26 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
35% identity, 82% coverage: 54:309/312 of query aligns to 56:313/334 of O58320
6ttbA Crystal structure of NAD-dependent formate dehydrogenase from staphylococcus aureus in complex with NAD
32% identity, 80% coverage: 58:308/312 of query aligns to 71:316/331 of 6ttbA
- binding nicotinamide-adenine-dinucleotide: V87 (≠ T74), N111 (≠ G98), V115 (= V102), F162 (= F153), G165 (= G156), R166 (≠ G157), I167 (= I158), Y185 (≠ K179), D186 (≠ N180), P187 (≠ A181), H214 (= H204), A215 (≠ C205), P216 (= P206), T221 (= T211), T242 (≠ V232), A243 (≠ G233), R244 (= R234), H292 (= H284), S294 (≠ A286), G295 (≠ W287)
4zgsA Identification of the pyruvate reductase of chlamydomonas reinhardtii (see paper)
33% identity, 82% coverage: 54:308/312 of query aligns to 65:335/346 of 4zgsA
- active site: S111 (≠ G98), R244 (= R234), D268 (= D258), E273 (= E263), H311 (= H284)
- binding nicotinamide-adenine-dinucleotide: Y110 (= Y97), G163 (= G156), A164 (≠ G157), I165 (= I158), D184 (≠ E177), C215 (= C205), P216 (= P206), L218 (≠ N208), S220 (≠ Q210), T221 (= T211), S243 (≠ G233), H311 (= H284), F314 (≠ W287)
7arzA Ternary complex of NAD-dependent formate dehydrogenase from physcomitrium patens
28% identity, 92% coverage: 26:312/312 of query aligns to 52:343/361 of 7arzA
- binding azide ion: T77 (≠ S50), P79 (≠ K52), F80 (≠ V53), L99 (≠ I69), V100 (≠ A70), T101 (≠ V71), G103 (≠ A73), V104 (≠ T74), R267 (= R234), H315 (= H284)
- binding nicotinamide-adenine-dinucleotide: V104 (≠ T74), N128 (≠ G98), V132 (= V102), G182 (= G156), R183 (≠ G157), I184 (= I158), D204 (vs. gap), R205 (vs. gap), T238 (≠ C205), P239 (= P206), Q243 (= Q210), N265 (≠ V232), A266 (≠ G233), R267 (= R234), D291 (= D258), H315 (= H284), S317 (≠ A286), G318 (≠ W287)
Sites not aligning to the query:
P87228 Putative D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; EC 1.1.1.95; EC 1.1.1.399 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
30% identity, 89% coverage: 34:310/312 of query aligns to 87:370/466 of P87228
- S87 (= S34) modified: Phosphoserine
- S258 (≠ N208) modified: Phosphoserine
7ewhA Crystal structure of human phgdh in complex with homoharringtonine (see paper)
29% identity, 80% coverage: 47:295/312 of query aligns to 46:289/302 of 7ewhA
- binding (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine: L146 (≠ F153), G147 (= G154), L148 (≠ K155), G149 (= G156), R150 (≠ G157), I151 (= I158), G152 (= G159), D170 (≠ E177), H201 (= H204), T202 (≠ C205), P203 (= P206)
6rihA Crystal structure of phgdh in complex with compound 9 (see paper)
29% identity, 80% coverage: 47:295/312 of query aligns to 46:289/302 of 6rihA