SitesBLAST
Comparing WP_025330258.1 NCBI__GCF_000600005.1:WP_025330258.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
4cpdA Alcohol dehydrogenase tadh from thermus sp. Atn1
32% identity, 99% coverage: 1:344/347 of query aligns to 1:345/346 of 4cpdA
- active site: C38 (= C38), G39 (= G39), S40 (= S40), H43 (≠ W43), H59 (= H59), E60 (= E60), C89 (= C89), C92 (= C92), C95 (= C95), C103 (= C103), G107 (= G112), D152 (= D151), T156 (= T155), K340 (= K339)
- binding nicotinamide-adenine-dinucleotide: G39 (= G39), S40 (= S40), T156 (= T155), G178 (= G177), P179 (≠ A178), V180 (= V179), D200 (≠ S199), R201 (= R200), R205 (= R204), A243 (≠ C243), V244 (= V244), V266 (= V266), V268 (≠ I268), L292 (≠ I290), A293 (= A291), F333 (≠ M332)
- binding zinc ion: C38 (= C38), H59 (= H59), C89 (= C89), C92 (= C92), C95 (= C95), C103 (= C103), D152 (= D151)
5ylnA Zinc dependent alcohol dehydrogenase 2 from streptococcus pneumonia - apo form
33% identity, 100% coverage: 1:347/347 of query aligns to 5:344/348 of 5ylnA
6schC Nadh-dependent variant of cbadh (see paper)
31% identity, 100% coverage: 1:347/347 of query aligns to 1:354/355 of 6schC
- active site: C37 (= C38), S39 (= S40), H42 (≠ W43), H59 (= H59), D150 (= D151)
- binding nicotinamide-adenine-dinucleotide: T38 (≠ G39), W110 (vs. gap), D150 (= D151), T154 (= T155), G174 (= G175), V178 (= V179), D198 (≠ S199), Y199 (≠ R200), R200 (≠ H201), A242 (≠ C243), G243 (≠ V244), G244 (= G245), N266 (≠ G267), Y267 (≠ I268), K340 (vs. gap)
- binding zinc ion: C37 (= C38), H59 (= H59), D150 (= D151)
1kevA Structure of NADP-dependent alcohol dehydrogenase (see paper)
31% identity, 99% coverage: 1:344/347 of query aligns to 1:351/351 of 1kevA
- active site: C37 (= C38), T38 (≠ G39), S39 (= S40), H42 (≠ W43), H59 (= H59), E60 (= E60), D89 (≠ C89), S92 (≠ C92), V95 (≠ C95), S103 (≠ C103), D150 (= D151), T154 (= T155), K346 (= K339)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: T38 (≠ G39), D150 (= D151), I175 (≠ D176), G176 (= G177), V178 (= V179), S199 (≠ R200), R200 (≠ H201), Y218 (≠ E219), A242 (≠ C243), G244 (= G245), N266 (≠ G267), Y267 (≠ I268), K340 (vs. gap)
- binding zinc ion: C37 (= C38), H59 (= H59), D150 (= D151)
P25984 NADP-dependent isopropanol dehydrogenase; CbADH; EC 1.1.1.80 from Clostridium beijerinckii (Clostridium MP) (see 3 papers)
31% identity, 99% coverage: 1:344/347 of query aligns to 1:351/351 of P25984
- C37 (= C38) binding Zn(2+)
- H59 (= H59) binding Zn(2+)
- E60 (= E60) binding Zn(2+)
- D150 (= D151) binding Zn(2+)
- IGAV 175:178 (≠ DGAV 176:179) binding NADP(+)
- GSR 198:200 (≠ SRH 199:201) binding NADP(+)
- Y218 (≠ E219) binding NADP(+)
- INY 265:267 (≠ VGI 266:268) binding NADP(+)
- K340 (vs. gap) binding NADP(+)
3fsrA Chimera of alcohol dehydrogenase by exchange of the cofactor binding domain res 153-295 of t. Brockii adh by c. Beijerinckii adh (see paper)
30% identity, 99% coverage: 1:345/347 of query aligns to 1:352/352 of 3fsrA
- active site: C37 (= C38), T38 (≠ G39), S39 (= S40), H42 (≠ W43), H59 (= H59), E60 (= E60), D89 (≠ C89), T92 (≠ C92), V95 (≠ C95), S103 (≠ C103), D150 (= D151), T154 (= T155), K346 (= K339)
- binding zinc ion: C37 (= C38), H59 (= H59), D150 (= D151)
3fplA Chimera of alcohol dehydrogenase by exchange of the cofactor binding domain res 153-295 of c. Beijerinckii adh by t. Brockii adh (see paper)
31% identity, 99% coverage: 1:344/347 of query aligns to 1:351/351 of 3fplA
- active site: C37 (= C38), T38 (≠ G39), S39 (= S40), H42 (≠ W43), H59 (= H59), E60 (= E60), D89 (≠ C89), S92 (≠ C92), V95 (≠ C95), S103 (≠ C103), D150 (= D151), T154 (= T155), K346 (= K339)
- binding zinc ion: C37 (= C38), H59 (= H59), D150 (= D151)
7ux4A Crystallographic snapshots of ternary complexes of thermophilic secondary alcohol dehydrogenase from thermoanaerobacter pseudoethanolicus reveal the dynamics of ligand exchange and the proton relay network. (see paper)
30% identity, 97% coverage: 11:345/347 of query aligns to 9:350/350 of 7ux4A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: D148 (= D151), T152 (= T155), G172 (= G175), I173 (≠ D176), G174 (= G177), P175 (≠ A178), V176 (= V179), R198 (≠ H201), G242 (= G245), V263 (= V266), N264 (≠ G267), Y265 (≠ I268), C293 (≠ A291)
- binding (1S,3S)-3-methylcyclohexan-1-ol: S37 (= S40), H57 (= H59), W108 (vs. gap), D148 (= D151)
- binding zinc ion: C35 (= C38), H57 (= H59), E58 (= E60), D148 (= D151)
1ykfA NADP-dependent alcohol dehydrogenase from thermoanaerobium brockii (see paper)
30% identity, 97% coverage: 11:345/347 of query aligns to 11:352/352 of 1ykfA
- active site: C37 (= C38), T38 (≠ G39), S39 (= S40), H42 (≠ W43), H59 (= H59), E60 (= E60), D89 (≠ C89), T92 (≠ C92), V95 (≠ C95), S103 (≠ C103), D150 (= D151), T154 (= T155), K346 (= K339)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: S39 (= S40), D150 (= D151), T154 (= T155), G174 (= G175), I175 (≠ D176), G176 (= G177), P177 (≠ A178), V178 (= V179), S199 (≠ R200), R200 (≠ H201), Y218 (≠ E219), I223 (≠ A224), N266 (≠ G267), Y267 (≠ I268)
- binding zinc ion: C37 (= C38), H59 (= H59), D150 (= D151)
1bxzB Crystal structure of a thermophilic alcohol dehydrogenase substrate complex from thermoanaerobacter brockii (see paper)
30% identity, 97% coverage: 11:345/347 of query aligns to 11:352/352 of 1bxzB
- active site: C37 (= C38), T38 (≠ G39), S39 (= S40), H42 (≠ W43), H59 (= H59), E60 (= E60), D89 (≠ C89), T92 (≠ C92), V95 (≠ C95), S103 (≠ C103), D150 (= D151), T154 (= T155), K346 (= K339)
- binding 2-butanol: H59 (= H59), D150 (= D151)
P14941 NADP-dependent isopropanol dehydrogenase; EC 1.1.1.80 from Thermoanaerobacter brockii (Thermoanaerobium brockii) (see 2 papers)
30% identity, 97% coverage: 11:345/347 of query aligns to 11:352/352 of P14941
- C37 (= C38) binding Zn(2+)
- H59 (= H59) binding Zn(2+)
- D150 (= D151) binding Zn(2+)
- IGPV 175:178 (≠ DGAV 176:179) binding NADP(+)
- GSR 198:200 (≠ SRH 199:201) binding NADP(+)
- Y218 (≠ E219) binding NADP(+)
- VNY 265:267 (≠ VGI 266:268) binding NADP(+)
- K340 (≠ R335) binding NADP(+)
7uutA Ternary complex crystal structure of secondary alcohol dehydrogenases from the thermoanaerobacter ethanolicus mutants c295a and i86a provides better understanding of catalytic mechanism (see paper)
30% identity, 97% coverage: 11:345/347 of query aligns to 11:352/352 of 7uutA
- binding (2R)-pentan-2-ol: S39 (= S40), H59 (= H59), A85 (≠ F85), W110 (vs. gap), D150 (= D151), C295 (≠ A291)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: C37 (= C38), T38 (≠ G39), S39 (= S40), D150 (= D151), T154 (= T155), G174 (= G175), G176 (= G177), P177 (≠ A178), V178 (= V179), S199 (≠ R200), R200 (≠ H201), A242 (≠ C243), G243 (≠ V244), G244 (= G245), I248 (≠ S249), V265 (= V266), N266 (≠ G267), Y267 (≠ I268), C295 (≠ A291), K340 (≠ R335)
- binding zinc ion: C37 (= C38), H59 (= H59), D150 (= D151)
7xy9A Cryo-em structure of secondary alcohol dehydrogenases tbsadh after carrier-free immobilization based on weak intermolecular interactions (see paper)
30% identity, 96% coverage: 11:344/347 of query aligns to 12:344/344 of 7xy9A