SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_025762291.1 NCBI__GCF_000566685.1:WP_025762291.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q89WV5 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110) (see paper)
58% identity, 98% coverage: 8:624/629 of query aligns to 22:638/648 of Q89WV5

query
sites
Q89WV5

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G263 (= G248) mutation to I: Loss of activity.
G266 (= G251) mutation to I: Great decrease in activity.
K269 (= K254) mutation to G: Great decrease in activity.
E414 (= E399) mutation to Q: Great decrease in activity.

P27550 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Escherichia coli (strain K12) (see paper)
56% identity, 99% coverage: 7:626/629 of query aligns to 22:643/652 of P27550

query
sites
P27550

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K609 (= K592) modified: N6-acetyllysine; by autocatalysis

Q8ZKF6 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
56% identity, 99% coverage: 7:626/629 of query aligns to 22:643/652 of Q8ZKF6

query
sites
Q8ZKF6

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N

-

V

T

S

T

I

K

K

W

W

F

Y

E

E

G

D

G

G

E

T

L

L

N

N

I

L

T

A

E

A

N

N

A

C

L

L

D

D

R

R

H

H

L

L

G

Q

D

E

K

N

G

G

D

D

Q

R

P

T

A

A

L

I

I

I

W

W

E

E

P

G

N

D

D

D

P

-

D

T

D

S

Q

Q

A

S

V

K

N

H

I

I

T

S

Y

Y

K

R

V

E

L

L

H

H

D

R

R

D

V

V

C

C

R

R

F

F

A

A

N

N

V

T

L

L

K

L

K

D

H

L

G

G

V

I

K

K

G

G

D

D

R

V

V

V

C

A

I

I

Y

Y

L

M

P

P

M

M

V

V

P

P

E

E

L

A

A

A

V

V

A

A

V

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

I

V

H

H

S

S

V

V

V

I

F

F

G

G

F

F

S

S

A

P

R

E

S

A

I

V

A

A

D

G

R

R

I

I

N

I

D

D

A

S

Q

S

C

S

V

R

M

L

V

V

I

I

T

T

S

A

D

D

G

E

A

G

F

V

R

R

G

A

P

G

K
x
R

V

S

I

I

P

P

M

L

K

K

D

K

T

N

V

V

D

D

A

A

L

L

E

K

Q

N

C

P

D

N

-

V

-

T

S

S

V

V

K

E

K

H

V

V

I

I

V

V

M

L

A

K

R

R

T

T

R

G

T

S

P

D

V

I

S

D

M

W

F

Q

K

E

G

G

R

R

D

D

L

L

W

W

E

R

E

D

E

L

V

I

K

E

H

K

V

A

D

S

S

P

N

E

C

H

P

Q

A

P

E

E

P

A

M

M

N

N

A

A

E

E

D

D

P

P

L

L

F

F

I

I

L

L

Y

Y

T

T

S

S

G

G

S

S

T

T

G

G

K

K

P

P

K

K

G

G

V

V

V

L

H

H

T

T

C

T

G

G

Y

Y

M

L

I

V

Y

Y

A

A

T

A

Y

T

T

T

F

F

T

K

N

Y

V

V

F

F

Q

D

Y

Y

D

H

P

P

G

G

E

D

I

I

H

Y

F

W

C

C

T

T

A

A

D

D

I

V

G

G

W

W

I

V

T
|
T

G

G

H

H

S

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

C

A

A

C

G

G

A

A

T

T

S

T

V

L

I

M

F

F

E

E

G

G

V

V

P

P

T
x
N

F

W

P

P

D

T

A

P

S

A

R

R

F

M

W

C

K

Q

I

V

V

V

E

D

R

K

H

H

K

Q

V

V

N

N

I

I

L

L

Y

Y

T

T

A
|
A

P

P

T

T

A

A

I

I

R

R

S

A

L

L

M

M

G

A

Y

E

G

G

L

D

H

K

F

A

V

I

K

E

D

G

H

T

D

D

L

R

T

S

S

S

L

L

K

R

K

I

L

L

G

G

S

S

V

V

G

G

E

E

P

P

I

I

N

N

Q

P

E

E

A

A

W

W

H

E

W

W

F

Y

K

W

L

K

N

K

I

I

G

G

R

K

N

E

I

K

C

C

P

P

L

V

V

V

D

D

T

T

W

W

Q

Q

T

T

E

E

T

T

G

G

I

F

M

M

I

I

S

T

P

P

L

L

A

P

G

G

I

A

T

I

P

E

E

L

K

K

P

A

S

G

F

S

A

A

T

T

L

R

P

P

L

F

P

F

G

G

V

V

Q

Q

P

P

V

A

L

L

V

V

D

D

E

N

K

E

G

G

D

H

E

P

L

Q

E

E

G

G

N

-

N

A

V

T

S

E

G

G

N

N

L

L

C

V

I

I

K

T

F

D

P

S

W

W

P

P

G

G

I

Q

I

A

R

R

T

T

I

L

Y

F

G

G

D

D

A

H

E

E

R

R

N

F

K

E

Q

Q

T

T

Y

Y

F

F

S

S

T

T

Y

F

P

K

G

N

M

M

Y

Y

F

F

T

S

G

G

D

D

G

G

C

A

L

R

R

R

D

D

E

E

D

D

G

G

F

Y

Y

Y

R

W

I

I

T

T

G

G

R

R

V

V

D
|
D

D

D

V

V

L

L

N

N

V

V

S
|
S

G
|
G

H

H

R
|
R

I

L

G

G

T

T

A

A

E

E

V

I

E

E

N

S

A

A

I

L

N

V

M

A

H

H

L

P

G

K

V

I

V

A

E

E

S

A

A

A

I

V

V

V

G

G

Y

I

P

P

H

H

D

A

I

I

K

K

G

G

Q

Q

G

A

I

I

Y

Y

A

A

F

Y

I

V

I

T

T

L

D

N

Q

H

K

G

P

E

E

E

-

P

D

S

P

P

E

E

M

L

F

Y

R

A

K

E

G

-

I

V

A

R

A

N

T

W

V

V

S
x
R

R

K

V

E

I

I

G

G

P

P

I

L

A

A

K

T

P

P

D

D

K

V

I

L

Q

H

F

W

V

T

S

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

I

I

S

A

E

A

G

G

D

D

F

T

D

S

S

N

L

L

G

G

D

D

T

T

S

S

T

T

L

L

L

A

D

D

P

P

A

G

I

V

V

V

E

E

E

K

I

L

K

L

E

E

R194 (≠ K178) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 3-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 2-fold reduction for CoA.
T311 (= T293) binding CoA
N335 (≠ T317) binding CoA
A357 (= A339) mutation to V: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 3-fold reduction for CoA.
D517 (= D500) mutation to G: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 10-fold reduction for CoA.; mutation to P: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold reduction in the affinity for ATP and 4.5-fold reduction for CoA.
S523 (= S506) binding CoA
G524 (= G507) mutation to L: No acetyl-coenzyme A synthetase activity.; mutation to S: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. Almost the same affinity as the wild-type for ATP, but 9-fold reduction in the affinity for CoA.
R526 (= R509) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 4-fold reduction for CoA.
R584 (≠ S567) binding CoA; mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 7-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 8-fold reduction for CoA.
K609 (= K592) modified: N6-acetyllysine; by Pat; mutation to A: No acetyl-coenzyme A synthetase activity.

5jrhA Crystal structure of salmonella enterica acetyl-coa synthetase (acs) in complex with camp and coenzyme a (see paper)
56% identity, 99% coverage: 7:626/629 of query aligns to 18:636/640 of 5jrhA

query
sites
5jrhA

E

E

E

Q

Y

Y

Q

E

D

T

T

K

Y

Y

N

K

Q

Q

S

S

V

I

E

N

D

D

P

P

E

D

G

T

F

F

W

W

A

G

E

E

I

Q

A

G

Q

K

Q

I

F

L

T

D

W

W

R

I

K

T

P

P

W

Y

K

Q

K

K

V

V

L

K

N

N

W

T

D

S

F

F

T

A

Q

P

G

G

-

N

-

V

T

S

T

I

K

K

W

W

F

Y

E

E

G

D

G

G

E

T

L

L

N

N

I

L

T

A

E

A

N

N

A

C

L

L

D

D

R

R

H

H

L

L

G

Q

D

E

K

N

G

G

D

D

Q

R

P

T

A

A

L

I

I

I

W
|
W

E

E

P

G

N

D

D

D

P

-

D

T

D

S

Q

Q

A

S

V

K

N

H

I

I

T

S

Y

Y

K

R

V

E

L

L

H

H

D

R

R

D

V

V

C

C

R

R

F

F

A

A

N

N

V

T

L

L

K

L

K

D

H

L

G

G

V

I

K

K

G

G

D

D

R

V

V

V

C

A

I

I

Y

Y

L

M

P

P

M

M

V

V

P

P

E
|
E

L

A

A

A

V

V

A

A

V

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

I

V

H

H

S

S

V

V

V

I

F
|
F

G
|
G

F

F

S

S

A

P

R

E

S

A

I

V

A

A

D
x
G

R

R

I

I

N

I

D

D

A

S

Q

S

C

S

V

R

M

L

V

V

I

I

T

T

S

A

D

D

G

E

A

G

F

V

R
|
R

G

A

P

G

K

R

V

S

I

I

P

P

M

L

K

K

D

K

T

N

V

V

D

D

A

A

L

L

E

K

Q

N

C

P

D

N

-

V

-

T

S

S

V

V

K

E

K

H

V

V

I

I

V

V

M

L

A

K

R

R

T

T

R

G

T

S

P

D

V

I

S

D

M

W

F

Q

K

E

G

G

R

R

D

D

L

L

W

W

E

R

E

D

E

L

V

I

K

E

H

K

V

A

D

S

S

P

N

E

C

H

P

Q

A

P

E

E

P

A

M

M

N

N

A

A

E

E

D

D

P

P

L

L

F

F

I

I

L

L

Y

Y

T
|
T

S

S

G

G

S

S

T

T

G

G

K
|
K

P
|
P

K

K

G

G

V

V

V

L

H

H

T

T

C

T

G

G

Y

Y

M

L

I

V

Y

Y

A

A

T

A

Y

T

T

T

F

F

T

K

N

Y

V

V

F

F

Q

D

Y

Y

D

H

P

P

G

G

E

D

I

I

H

Y

F

W

C

C

T

T

A

A

D

D

I

V

G

G

W

W

I

V

T

T

G

G

H

H

S

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

C

A

A

C

G

G

A

A

T

T

S

T

V

L

I

M

F

F

E

E

G

G

V

V

P

P

T

N

F

W

P

P

D

T

A

P

S

A

R

R

F

M

W

C

K

Q

I

V

V

V

E

D

R

K

H

H

K

Q

V

V

N

N

I

I

L

L

Y

Y

T

T

A

A

P

P

T

T

A

A

I

I

R

R

S

A

L

L

M

M

G

A

Y

E

G

G

L

D

H

K

F

A

V

I

K

E

D

G

H

T

D

D

L

R

T

S

S

S

L

L

K

R

K

I

L

L

G

G

S

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

Q

P

E

E

A

A

W

W

H

E

W

W

F

Y

K

W

L

K

N

K

I

I

G

G

R

K

N

E

I

K

C

C

P

P

L

V

V

V

D

D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

I

F

M

M

I

I

S

T

P

P

L

L

A

P

G

G

I

A

T

I

P

E

E

L

K

K

P

A

S

G

F

S

A

A

T

T

L

R

P

P

L

F

P

F

G

G

V

V

Q

Q

P

P

V

A

L

L

V

V

D

D

E

N

K

E

G

G

D

H

E

P

L

Q

E

E

G

G

N

-

N

A

V

T

S

E

G

G

N

N

L

L

C

V

I

I

K

T

F

D

P

S

W

W

P

P

G

G

I

Q

I

A

R

R

T

T

I

L

Y

F

G

G

D

D

A

H

E

E

R

R

N

F

K

E

Q

Q

T

T

Y

Y

F

F

S

S

T

T

Y

F

P

K

G

N

M

M

Y

Y

F

F

T

S

G

G

D
|
D

G

G

C

A

L

R

R

R

D

D

E

E

D

D

G

G

F

Y

Y

Y

R

W

I
|
I

T

T

G

G

R

R

V

V

D

D

V

V

L

L

N
|
N

V

V

S
|
S

G

G

H

H

R
|
R

I

L

G

G

T

T

A

A

E

E

V

I

E

E

N

S

A

A

I

L

N
x
V

M

A

H
|
H

L

P

G

K

V
x
I

V

A

E

E

S

A

A

A

I

V

V

V

G

G

Y

I

P

P

H

H

D

A

I

I

K

K

G

G

Q

Q

G

A

I

I

Y

Y

A

A

F

Y

I

V

I

T

T

L

D

N

Q

H

K

G

P

E

E

E

-

P

D

S

P

P

E

E

M

L

F

Y

R

A

K

E

G

-

I

V

A

R

A

N

T

W

V

V

S
x
R

R

K

V

E

I

I

G

G

P
|
P

I

L

A

A

K

T

P

P

D

D

K

V

I

L

Q

H

F

W

V

T

S

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

I

I

S

A

E

A

G

G

D

D

F

-

D

-

S

-

L

L

G

G

D

D

T

T

S

S

T

T

L

L

L

A

D

D

P

P

A

G

I

V

V

V

E

E

E

K

I

L

K

L

E

E

active site: T260 (= T246), T412 (= T398), E413 (= E399), N517 (= N504), R522 (= R509), K605 (= K592)
binding (r,r)-2,3-butanediol: W93 (= W80), E140 (= E128), G169 (≠ D157), K266 (= K252), P267 (= P253)
binding adenosine-3',5'-cyclic-monophosphate: G383 (= G369), E384 (= E370), P385 (= P371), T408 (= T394), W409 (= W395), W410 (= W396), Q411 (= Q397), T412 (= T398), D496 (= D483), I508 (= I495), N517 (= N504), R522 (= R509)
binding coenzyme a: F159 (= F147), G160 (= G148), G161 (= G149), R187 (= R175), S519 (= S506), R580 (≠ S567), P585 (= P572)
binding magnesium ion: V533 (≠ N520), H535 (= H522), I538 (≠ V525)

2p20A Acetyl-coa synthetase, r584a mutation (see paper)
55% identity, 99% coverage: 7:626/629 of query aligns to 18:637/641 of 2p20A

query
sites
2p20A

E

E

E

Q

Y

Y

Q

E

D

T

T

K

Y

Y

N

K

Q

Q

S

S

V

I

E

N

D

D

P

P

E

D

G

T

F

F

W

W

A

G

E

E

I

Q

A

G

Q

K

Q

I

F

L

T

D

W

W

R

I

K

T

P

P

W

Y

K

Q

K

K

V

V

L

K

N

N

W

T

D

S

F

F

T

A

Q

P

G

G

-

N

-

V

T

S

T

I

K

K

W

W

F

Y

E

E

G

D

G

G

E

T

L

L

N

N

I

L

T

A

E

A

N

N

A

C

L

L

D

D

R

R

H

H

L

L

G

Q

D

E

K

N

G

G

D

D

Q

R

P

T

A

A

L

I

I

I

W

W

E

E

P

G

N

D

D

D

P

-

D

T

D

S

Q

Q

A

S

V

K

N

H

I

I

T

S

Y

Y

K

R

V

E

L

L

H

H

D

R

R

D

V

V

C

C

R

R

F

F

A

A

N

N

V

T

L

L

K

L

K

D

H

L

G

G

V

I

K

K

G

G

D

D

R

V

V

V

C

A

I

I

Y

Y

L

M

P

P

M

M

V

V

P

P

E

E

L

A

A

A

V

V

A

A

V

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

I

V

H

H

S

S

V

V

V

I

F

F

G

G

F

F

S

S

A

P

R

E

S

A

I

V

A

A

D

G

R

R

I

I

N

I

D

D

A

S

Q

S

C

S

V

R

M

L

V

V

I

I

T

T

S

A

D

D

G

E

A

G

F

V

R

R

G

A

P

G

K

R

V

S

I

I

P

P

M

L

K

K

D

K

T

N

V

V

D

D

A

A

L

L

E

K

Q

N

C

P

D

N

-

V

-

T

S

S

V

V

K

E

K

H

V

V

I

I

V

V

M

L

A

K

R

R

T

T

R

G

T

S

P

D

V

I

S

D

M

W

F

Q

K

E

G

G

R

R

D

D

L

L

W

W

E

R

E

D

E

L

V

I

K

E

H

K

V

A

D

S

S

P

N

E

C

H

P

Q

A

P

E

E

P

A

M

M

N

N

A

A

E

E

D

D

P

P

L

L

F

F

I

I

L

L

Y

Y

T
|
T

S

S

G

G

S

S

T

T

G

G

K

K

P

P

K

K

G

G

V

V

V

L

H

H

T

T

C

T

G

G

Y

Y

M

L

I

V

Y

Y

A

A

T

A

Y

T

T

T

F

F

T

K

N

Y

V

V

F

F

Q

D

Y

Y

D

H

P

P

G

G

E

D

I

I

H

Y

F

W

C

C

T

T

A

A

D

D

I

V

G

G

W

W

I

V

T

T

G

G

H

H

S

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

C

A

A

C

G

G

A

A

T

T

S

T

V

L

I

M

F

F

E

E

G

G

V

V

P

P

T

N

F

W

P

P

D

T

A

P

S

A

R

R

F

M

W

C

K

Q

I

V

V

V

E

D

R

K

H

H

K

Q

V

V

N

N

I

I

L

L

Y

Y

T

T

A

A

P

P

T

T

A

A

I

I

R

R

S

A

L

L

M

M

G

A

Y

E

G

G

L

D

H

K

F

A

V

I

K

E

D

G

H

T

D

D

L

R

T

S

S

S

L

L

K

R

K

I

L

L

G

G

S

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

Q

P

E

E

A

A

W

W

H

E

W

W

F

Y

K

W

L

K

N

K

I

I

G

G

R

K

N

E

I

K

C

C

P

P

L

V

V

V

D

D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

I

F

M

M

I

I

S

T

P

P

L

L

A

P

G

G

I

A

T

I

P

E

E

L

K

K

P

A

S

G

F

S

A

A

T

T

L

R

P

P

L

F

P

F

G

G

V

V

Q

Q

P

P

V

A

L

L

V

V

D

D

E

N

K

E

G

G

D

H

E

P

L

Q

E

E

G

G

N

-

N

A

V

T

S

E

G

G

N

N

L

L

C

V

I

I

K

T

F

D

P

S

W

W

P

P

G

G

I

Q

I

A

R

R

T

T

I

L

Y

F

G

G

D

D

A

H

E

E

R

R

N

F

K

E

Q

Q

T

T

Y

Y

F

F

S

S

T

T

Y

F

P

K

G

N

M

M

Y

Y

F

F

T

S

G

G

D
|
D

G

G

C

A

L

R

R

R

D

D

E

E

D

D

G

G

F

Y

Y

Y

R

W

I
|
I

T

T

G

G

R
|
R

V

V

D

D

V

V

L

L

N
|
N

V

V

S

S

G

G

H

H

R
|
R

I

L

G

G

T

T

A

A

E

E

V

I

E

E

N

S

A

A

I

L

N

V

M

A

H

H

L

P

G

K

V

I

V

A

E

E

S

A

A

A

I

V

V

V

G

G

Y

I

P

P

H

H

D

A

I

I

K

K

G

G

Q

Q

G

A

I

I

Y

Y

A

A

F

Y

I

V

I

T

T

L

D

N

Q

H

K

G

P

E

E

E

-

P

D

S

P

P

E

E

M

L

F

Y

R

A

K

E

G

-

I

V

A

R

A

N

T

W

V

V

S

A

R

K

V

E

I

I

G

G

P

P

I

L

A

A

K

T

P

P

D

D

K

V

I

L

Q

H

F

W

V

T

S

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

I

I

S

A

E

A

G

G

D

-

F

-

D

D

S

T

L

S

G

N

D

D

T

T

S

S

T

T

L

L

L

A

D

D

P

P

A

G

I

V

V

V

E

E

E

K

I

L

K

L

E

E

active site: T260 (= T246), T412 (= T398), E413 (= E399), N517 (= N504), R522 (= R509), K605 (= K592)
binding adenosine-5'-monophosphate-propyl ester: G383 (= G369), E384 (= E370), P385 (= P371), T408 (= T394), W409 (= W395), W410 (= W396), Q411 (= Q397), T412 (= T398), D496 (= D483), I508 (= I495), R511 (= R498), R522 (= R509)

2p2fA Acetyl-coa synthetase, wild-type with acetate, amp, and coa bound (see paper)
55% identity, 99% coverage: 7:626/629 of query aligns to 17:633/637 of 2p2fA

query
sites
2p2fA

E

E

E

Q

Y

Y

Q

E

D

T

T

K

Y

Y

N

K

Q

Q

S

S

V

I

E

N

D

D

P

P

E

D

G

T

F

F

W

W

A

G

E

E

I

Q

A

G

Q

K

Q

I

F

L

T

D

W

W

R

I

K

T

P

P

W

Y

K

Q

K

K

V

V

L

K

N

N

W

T

D

S

F

F

T

A

Q

P

G

G

-

N

-

V

T

S

T

I

K

K

W

W

F

Y

E

E

G

D

G

G

E

T

L

L

N

N

I

L

T

A

E

A

N

N

A

C

L

L

D

D

R

R

H

H

L

L

G

Q

D

E

K

N

G

G

D

D

Q

R

P

T

A

A

L

I

I

I

W

W

E

E

P

G

N

D

D

D

P

-

D

T

D

S

Q

Q

A

S

V

K

N

H

I

I

T

S

Y

Y

K

R

V

E

L

L

H

H

D

R

R

D

V

V

C

C

R

R

F

F

A

A

N

N

V

T

L

L

K

L

K

D

H

L

G

G

V

I

K

K

G

G

D

D

R

V

V

V

C

A

I

I

Y

Y

L

M

P

P

M

M

V

V

P

P

E

E

L

A

A

A

V

V

A

A

V

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

I

V

H

H

S

S

V

V

V

I

F
|
F

G

G

F

F

S

S

A

P

R

E

S

A

I

V

A

A

D

G

R

R

I

I

N

I

D

D

A

S

Q

S

C

S

V

R

M

L

V

V

I

I

T

T

S

A

D

D

G

E

A

G

F

V

R
|
R

G

A

P

G

K

R

V

S

I

I

P

P

M

L

K

K

D

K

T

N

V

V

D

D

A

A

L

L

E

K

Q

N

C

P

D

N

-

V

-

T

S

S

V

V

K

E

K

H

V

V

I

I

V

V

M

L

A

K

R

R

T

T

R

G

T

S

P

D

V

I

S

D

M

W

F

Q

K

E

G

G

R

R

D

D

L

L

W

W

E

R

E

D

E

L

V

I

K

E

H

K

V

A

D

S

S

P

N

E

C

H

P

Q

A

P

E

E

P

A

M

M

N

N

A

A

E

E

D

D

P

P

L

L

F

F

I

I

L

L

Y

Y

T
|
T

S

S

G

G

S

S

T

T

G

G

K

K

P

P

K

K

G

G

V

V

V

L

H

H

T

T

C

T

G

G

Y

Y

M

L

I

V

Y

Y

A

A

T

A

Y

T

T

T

F

F

T

K

N

Y

V

V

F

F

Q

D

Y

Y

D

H

P

P

G

G

E

D

I

I

H

Y

F

W

C

C

T

T

A

A

D

D

I

V

G

G

W
|
W

I

V

T
|
T

G

G

H

H

S

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

C

A

A

C

G

G

A

A

T

T

S

T

V

L

I

M

F

F

E

E

G

G

V

V

P
|
P

T

N

F

W

P

P

D

T

A

P

S

A

R

R

F

M

W

C

K

Q

I

V

V

V

E

D

R

K

H

H

K

Q

V

V

N

N

I

I

L

L

Y

Y

T

T

A
|
A

P

P

T

T

A
|
A

I

I

R

R

S

A

L

L

M

M

G

A

Y

E

G

G

L

D

H

K

F

A

V

I

K

E

D

G

H

T

D

D

L

R

T

S

S

S

L

L

K

R

K

I

L

L

G

G

S

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

Q

P

E

E

A

A

W

W

H

E

W

W

F

Y

K

W

L

K

N

K

I

I

G

G

R

K

N

E

I

K

C

C

P

P

L

V

V

V

D

D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

I

F

M

M

I

I

S

T

P

P

L

L

A

P

G

G

I

A

T

I

P

E

E

L

K

K

P

A

S

G

F

S

A

A

T

T

L

R

P

P

L

F

P

F

G

G

V

V

Q

Q

P

P

V

A

L

L

V

V

D

D

E

N

K

E

G

G

D

H

E

P

L

Q

E

E

G

G

N

-

N

A

V

T

S

E

G

G

N

N

L

L

C

V

I

I

K

T

F

D

P

S

W

W

P

P

G

G

I

Q

I

A

R

R

T

T

I

L

Y

F

G

G

D

D

A

H

E

E

R

R

N

F

K

E

Q

Q

T

T

Y

Y

F

F

S

S

T

T

Y

F

P

K

G

N

M

M

Y

Y

F

F

T

S

G

G

D
|
D

G

G

C

A

L

R

R

R

D

D

E

E

D

D

G

G

F

Y

Y

Y

R

W

I
|
I

T

T

G

G

R
|
R

V

V

D

D

V

V

L

L

N
|
N

V

V

S
|
S

G

G

H

H

R
|
R

I

L

G

G

T

T

A

A

E

E

V

I

E

E

N

S

A

A

I

L

N

V

M

A

H

H

L

P

G

K

V

I

V

A

E

E

S

A

A

A

I

V

V

V

G

G

Y

I

P

P

H

H

D

A

I

I

K

K

G

G

Q

Q

G

A

I

I

Y

Y

A

A

F

Y

I

V

I

T

T

L

D

N

Q

H

K

G

P

E

E

E

-

P

D

S

P

P

E

E

M

L

F

Y

R

A

K

E

G

-

I

V

A

R

A

N

T

W

V

V

S
x
R

R

K

V

E

I

I

G

G

P
|
P

I

L

A

A

K

T

P

P

D

D

K

V

I

L

Q

H

F

W

V

T

S

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

I

I

S

A

E

A

G

G

D

D

F

-

D

-

S

-

L

-

G

-

D

T

T

S

S

S

T

T

L

L

L

A

D

D

P

P

A

G

I

V

V

V

E

E

E

K

I

L

K

L

E

E

active site: T259 (= T246), T411 (= T398), E412 (= E399), N516 (= N504), R521 (= R509), K604 (= K592)
binding adenosine monophosphate: G382 (= G369), E383 (= E370), P384 (= P371), T407 (= T394), W408 (= W395), W409 (= W396), Q410 (= Q397), T411 (= T398), D495 (= D483), I507 (= I495), R510 (= R498), N516 (= N504), R521 (= R509)
binding coenzyme a: F158 (= F147), R186 (= R175), W304 (= W291), T306 (= T293), P329 (= P316), A352 (= A339), A355 (= A342), S518 (= S506), R579 (≠ S567), P584 (= P572)

1pg3A Acetyl coa synthetase, acetylated on lys609 (see paper)
54% identity, 99% coverage: 7:626/629 of query aligns to 18:630/634 of 1pg3A

query
sites
1pg3A

E

E

E

Q

Y

Y

Q

E

D

T

T

K

Y

Y

N

K

Q

Q

S

S

V

I

E

N

D

D

P

P

E

D

G

T

F

F

W

W

A

G

E

E

I

Q

A

G

Q

K

Q

I

F

L

T

D

W

W

R

I

K

T

P

P

W

Y

K

Q

K

K

V

V

L

K

N

N

W

T

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active site: T260 (= T246), T412 (= T398), E413 (= E399), N517 (= N504), R522 (= R509), K605 (= K592)
binding coenzyme a: F159 (= F147), G160 (= G148), R187 (= R175), R190 (≠ K178), A301 (= A287), T307 (= T293), P330 (= P316), A356 (= A342), S519 (= S506), R580 (≠ S567), P585 (= P572)
binding magnesium ion: V533 (≠ N520), H535 (= H522), I538 (≠ V525)
binding adenosine-5'-monophosphate-propyl ester: G383 (= G369), E384 (= E370), P385 (= P371), T408 (= T394), W409 (= W395), W410 (= W396), Q411 (= Q397), T412 (= T398), D496 (= D483), R511 (= R498), R522 (= R509)

Q9NUB1 Acetyl-coenzyme A synthetase 2-like, mitochondrial; Acetate--CoA ligase 2; Acetyl-CoA synthetase 2; AceCS2; Acyl-CoA synthetase short-chain family member 1; Propionate--CoA ligase; EC 6.2.1.1; EC 6.2.1.17 from Homo sapiens (Human) (see 3 papers)
52% identity, 98% coverage: 8:624/629 of query aligns to 57:674/689 of Q9NUB1

query
sites
Q9NUB1

E

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&nb