SitesBLAST
Comparing WP_025763018.1 NCBI__GCF_000566685.1:WP_025763018.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
6slbAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
38% identity, 97% coverage: 8:259/259 of query aligns to 8:257/257 of 6slbAAA
- active site: Q64 (≠ A65), F69 (≠ T70), L80 (= L81), N84 (= N86), A108 (= A110), S111 (= S113), A130 (≠ M132), F131 (= F133), L136 (= L138), P138 (= P140), D139 (= D141), A224 (≠ D226), G234 (≠ S236)
- binding (~{E})-6-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-6-oxidanylidene-hex-3-enoic acid: R58 (≠ T59), A62 (≠ S63), Q64 (≠ A65), D65 (= D66), L66 (= L67), Y76 (≠ V77), A108 (= A110), F131 (= F133), D139 (= D141)
6slaAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
37% identity, 97% coverage: 8:259/259 of query aligns to 5:245/245 of 6slaAAA
- active site: Q61 (≠ A65), L68 (= L81), N72 (= N86), A96 (= A110), S99 (= S113), A118 (≠ M132), F119 (= F133), L124 (= L138), P126 (= P140), N127 (≠ D141), A212 (≠ D226), G222 (≠ S236)
- binding ~{S}-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyl] 2-(2,5-dihydrooxepin-7-yl)ethanethioate: L21 (≠ H24), A59 (≠ S63), Q61 (≠ A65), D62 (= D66), L63 (= L67), L68 (= L81), Y71 (= Y84), A94 (≠ V108), G95 (= G109), A96 (= A110), F119 (= F133), I122 (= I136), L124 (= L138), N127 (≠ D141), F234 (= F248), K237 (= K251)
5zaiC Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
34% identity, 97% coverage: 10:259/259 of query aligns to 10:259/259 of 5zaiC
- active site: A65 (= A65), F70 (vs. gap), S82 (≠ D78), R86 (= R82), G110 (≠ A110), E113 (≠ S113), P132 (≠ M132), E133 (≠ F133), I138 (≠ L138), P140 (= P140), G141 (≠ D141), A226 (≠ D226), F236 (≠ S236)
- binding coenzyme a: K24 (≠ V23), L25 (≠ H24), A63 (≠ S63), G64 (= G64), A65 (= A65), D66 (= D66), I67 (≠ L67), P132 (≠ M132), R166 (= R166), F248 (= F248), K251 (= K251)
5jbxB Crystal structure of liuc in complex with coenzyme a and malonic acid (see paper)
31% identity, 91% coverage: 24:259/259 of query aligns to 26:261/261 of 5jbxB
- active site: A67 (= A65), R72 (≠ T70), L84 (≠ Y85), R88 (≠ I89), G112 (≠ A110), E115 (≠ S113), T134 (≠ M132), E135 (≠ F133), I140 (≠ L138), P142 (= P140), G143 (≠ D141), A228 (≠ D226), L238 (≠ S236)
- binding coenzyme a: R26 (≠ H24), A28 (= A26), A65 (≠ S63), D68 (= D66), L69 (= L67), K70 (= K68), L110 (≠ V108), G111 (= G109), T134 (≠ M132), E135 (≠ F133), L138 (≠ I136), R168 (= R166)
Sites not aligning to the query:
2uzfA Crystal structure of staphylococcus aureus 1,4-dihydroxy-2-naphthoyl coa synthase (menb) in complex with acetoacetyl coa (see paper)
31% identity, 98% coverage: 2:256/259 of query aligns to 7:253/260 of 2uzfA
- active site: G70 (≠ A65), R80 (= R82), L84 (≠ N86), G108 (≠ A110), V111 (≠ S113), T130 (≠ M132), G131 (≠ F133), S136 (≠ L138), D138 (≠ P140), A139 (≠ D141), A225 (≠ L225), Y233 (≠ K237)
- binding acetoacetyl-coenzyme a: V28 (= V23), R29 (≠ H24), S68 (= S63), G69 (= G64), G70 (≠ A65), D71 (= D66), Y104 (≠ L106), G108 (≠ A110)
Q5HH38 1,4-dihydroxy-2-naphthoyl-CoA synthase; DHNA-CoA synthase; EC 4.1.3.36 from Staphylococcus aureus (strain COL) (see paper)
31% identity, 98% coverage: 2:256/259 of query aligns to 12:266/273 of Q5HH38
- R34 (≠ H24) binding in other chain
- SGGDQ 73:77 (≠ SGADL 63:67) binding in other chain
- S149 (≠ L138) binding in other chain
1ey3A Structure of enoyl-coa hydratase complexed with the substrate dac-coa (see paper)
30% identity, 93% coverage: 16:257/259 of query aligns to 18:256/258 of 1ey3A
- active site: A66 (= A65), M71 (≠ T70), S81 (≠ N86), L85 (≠ M90), G109 (≠ A110), E112 (≠ S113), P131 (≠ M132), E132 (≠ F133), T137 (≠ L138), P139 (= P140), G140 (≠ D141), K225 (≠ D226), F235 (≠ S236)
- binding 4-(n,n-dimethylamino)cinnamoyl-coa: K24 (≠ H22), L26 (≠ H24), A28 (= A26), A64 (≠ S63), G65 (= G64), A66 (= A65), D67 (= D66), I68 (≠ L67), L85 (≠ M90), W88 (vs. gap), G109 (≠ A110), P131 (≠ M132), L135 (≠ I136), G140 (≠ D141)
1dubA 2-enoyl-coa hydratase, data collected at 100 k, ph 6.5 (see paper)
30% identity, 93% coverage: 16:257/259 of query aligns to 20:258/260 of 1dubA
- active site: A68 (= A65), M73 (≠ T70), S83 (≠ N86), L87 (≠ M90), G111 (≠ A110), E114 (≠ S113), P133 (≠ M132), E134 (≠ F133), T139 (≠ L138), P141 (= P140), G142 (≠ D141), K227 (≠ D226), F237 (≠ S236)
- binding acetoacetyl-coenzyme a: K26 (≠ H22), A27 (≠ V23), L28 (≠ H24), A30 (= A26), A66 (≠ S63), A68 (= A65), D69 (= D66), I70 (≠ L67), Y107 (≠ L106), G110 (= G109), G111 (≠ A110), E114 (≠ S113), P133 (≠ M132), E134 (≠ F133), L137 (≠ I136), G142 (≠ D141), F233 (≠ Q232), F249 (= F248)
P14604 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus (Rat) (see 3 papers)
30% identity, 93% coverage: 16:257/259 of query aligns to 50:288/290 of P14604
- E144 (≠ S113) mutation to D: Reduces activity 50-fold.; mutation to Q: Reduces activity 3300-fold.
- E164 (≠ F133) mutation to D: Reduces activity 1250-fold.; mutation to Q: Reduces activity 330000-fold.
Sites not aligning to the query:
- 1:29 modified: transit peptide, Mitochondrion
3q0jC Crystal structure of the mycobacterium tuberculosis crotonase in complex with the inhibitor acetoacetylcoa
28% identity, 98% coverage: 2:256/259 of query aligns to 3:254/255 of 3q0jC
- active site: A65 (= A65), M70 (≠ T70), T80 (≠ L81), F84 (≠ Y85), G108 (≠ A110), E111 (≠ S113), P130 (≠ M132), E131 (≠ F133), V136 (≠ L138), P138 (= P140), G139 (≠ D141), L224 (≠ D226), F234 (≠ S236)
- binding acetoacetyl-coenzyme a: Q23 (≠ H22), A24 (≠ V23), L25 (≠ H24), A27 (= A26), A63 (≠ S63), G64 (= G64), A65 (= A65), D66 (= D66), I67 (≠ L67), K68 (= K68), M70 (≠ T70), F84 (≠ Y85), G107 (= G109), G108 (≠ A110), E111 (≠ S113), P130 (≠ M132), E131 (≠ F133), P138 (= P140), G139 (≠ D141), M140 (≠ A142)
3q0gC Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
28% identity, 98% coverage: 2:256/259 of query aligns to 3:254/255 of 3q0gC
- active site: A65 (= A65), M70 (≠ T70), T80 (≠ L81), F84 (≠ Y85), G108 (≠ A110), E111 (≠ S113), P130 (≠ M132), E131 (≠ F133), V136 (≠ L138), P138 (= P140), G139 (≠ D141), L224 (≠ D226), F234 (≠ S236)
- binding coenzyme a: L25 (≠ H24), A63 (≠ S63), I67 (≠ L67), K68 (= K68), Y104 (≠ L106), P130 (≠ M132), E131 (≠ F133), L134 (≠ I136)
3h81A Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis (see paper)
28% identity, 98% coverage: 2:256/259 of query aligns to 2:253/256 of 3h81A
- active site: A64 (= A65), M69 (≠ T70), T79 (≠ L81), F83 (≠ Y85), G107 (≠ A110), E110 (≠ S113), P129 (≠ M132), E130 (≠ F133), V135 (≠ L138), P137 (= P140), G138 (≠ D141), L223 (≠ D226), F233 (≠ S236)
- binding calcium ion: F233 (≠ S236), Q238 (≠ A241)
2hw5C The crystal structure of human enoyl-coenzyme a (coa) hydratase short chain 1, echs1
30% identity, 93% coverage: 16:257/259 of query aligns to 20:258/260 of 2hw5C
- active site: A68 (= A65), M73 (≠ T70), S83 (≠ N86), L87 (≠ M90), G111 (≠ A110), E114 (≠ S113), P133 (≠ M132), E134 (≠ F133), T139 (≠ L138), P141 (= P140), G142 (≠ D141), K227 (≠ D226), F237 (≠ S236)
- binding crotonyl coenzyme a: K26 (≠ H22), A27 (≠ V23), L28 (≠ H24), A30 (= A26), K62 (≠ T59), I70 (≠ L67), F109 (≠ V108)
1mj3A Crystal structure analysis of rat enoyl-coa hydratase in complex with hexadienoyl-coa (see paper)
30% identity, 93% coverage: 16:257/259 of query aligns to 20:256/258 of 1mj3A
- active site: A68 (= A65), M73 (≠ T70), S83 (≠ N86), L85 (≠ M88), G109 (≠ A110), E112 (≠ S113), P131 (≠ M132), E132 (≠ F133), T137 (≠ L138), P139 (= P140), G140 (≠ D141), K225 (≠ D226), F235 (≠ S236)
- binding hexanoyl-coenzyme a: K26 (≠ H22), A27 (≠ V23), L28 (≠ H24), A30 (= A26), A66 (≠ S63), G67 (= G64), A68 (= A65), D69 (= D66), I70 (≠ L67), G109 (≠ A110), P131 (≠ M132), E132 (≠ F133), L135 (≠ I136), G140 (≠ D141)
2dubA Enoyl-coa hydratase complexed with octanoyl-coa (see paper)
30% identity, 93% coverage: 16:257/259 of query aligns to 19:252/254 of 2dubA
- active site: A67 (= A65), M72 (≠ T70), S82 (≠ N86), G105 (≠ A110), E108 (≠ S113), P127 (≠ M132), E128 (≠ F133), T133 (≠ L138), P135 (= P140), G136 (≠ D141), K221 (≠ D226), F231 (≠ S236)
- binding octanoyl-coenzyme a: K25 (≠ H22), A26 (≠ V23), L27 (≠ H24), A29 (= A26), A65 (≠ S63), A67 (= A65), D68 (= D66), I69 (≠ L67), K70 (= K68), G105 (≠ A110), E108 (≠ S113), P127 (≠ M132), E128 (≠ F133), G136 (≠ D141), A137 (= A142)
Q8GYN9 1,4-dihydroxy-2-naphthoyl-CoA synthase, peroxisomal; DHNS; Enoyl-CoA hydratase/isomerase D; ECHID; Naphthoate synthase; EC 4.1.3.36 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
31% identity, 95% coverage: 11:256/259 of query aligns to 85:330/337 of Q8GYN9
Sites not aligning to the query:
- 20 H→V: Loss of peroxisomal targeting.
3q0gD Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
27% identity, 98% coverage: 2:256/259 of query aligns to 2:249/250 of 3q0gD