SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_026093942.1 NCBI__GCF_000816635.1:WP_026093942.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

7kb1C Complex of o-acety-l-homoserine aminocarboxypropyltransferase (mety) from thermotoga maritima and a key reaction intermediate (see paper)
43% identity, 99% coverage: 3:408/409 of query aligns to 7:425/428 of 7kb1C

query
sites
7kb1C

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binding pyridoxal-5'-phosphate: Y57 (= Y51), R59 (= R53)
binding (2E)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)imino]but-3-enoic acid: G87 (= G81), Q88 (≠ M82), Y112 (= Y106), N160 (= N152), D185 (= D177), S206 (= S199), T208 (≠ S201), K209 (= K202), N369 (= N352), I370 (= I353), R404 (= R387)

7kb1A Complex of o-acety-l-homoserine aminocarboxypropyltransferase (mety) from thermotoga maritima and a key reaction intermediate (see paper)
43% identity, 99% coverage: 3:408/409 of query aligns to 7:425/428 of 7kb1A

query
sites
7kb1A

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binding (2E)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)imino]but-3-enoic acid: Y57 (= Y51), R59 (= R53), G87 (= G81), Q88 (≠ M82), Y112 (= Y106), N160 (= N152), D185 (= D177), S206 (= S199), T208 (≠ S201), K209 (= K202), N369 (= N352), I370 (= I353), R404 (= R387)

8wkoA Crystal structure of o-acetylhomoserine sulfhydrylase from lactobacillus plantarum in the closed form (see paper)
40% identity, 100% coverage: 2:409/409 of query aligns to 8:425/425 of 8wkoA

query
sites
8wkoA

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L

L

L

I

R

R

L

I

S

S

V

V

G

G

I

V

E

E

D

N

V

A

E

D

D

D

I

L

I

I

A

A

D

D

I

L

D

D

S

Q

A

A

L

L

K

A

E

Q

L

V

binding (2S)-2-amino-6-[[3-hydroxy-2-methyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]hexanoic acid: G87 (= G81), S88 (≠ M82), Y112 (= Y106), E155 (= E148), D184 (= D177), T186 (= T179), S206 (= S199), A207 (≠ T200), T208 (≠ S201), F209 (≠ Y203), G212 (= G206), M217 (≠ I211), V369 (≠ I353), A370 (≠ G354)
binding proline: H213 (≠ T207), Q284 (≠ T269), S288 (≠ T273)

2ctzA Crystal structure of o-acetyl homoserine sulfhydrylase from thermus thermophilus hb8
38% identity, 99% coverage: 1:406/409 of query aligns to 1:420/421 of 2ctzA

query
sites
2ctzA

M

M

K

R

F

F

N

E

T

T

K

L

L

Q

I

L

H

H

G

A

N

G

A

Y

R

E

L

P

D

E

G

P

T

T

G

T

-

L

A

S

T

R

N

Q

T

V

P

P

V

I

Y

Y

L

P

S

T

N

T

A

S

Y

Y

A

V

H

F

T

K

S

S

A

P

E

E

K

H

L

A

E

A

K

N

I

L

F

F

Q

A

G

L

K

K

D

E

M

F

G

G

Y

N

A

I

Y

Y

T

S

R
|
R

I

I

S

M

N

N

P

P

T

T

V

V

T

D

S

V

F

L

E

E

R

K

R

R

I

L

A

A

S

A

I

L

E

E

N

G

G

G

M

K

V

A

A

A

T

L

S

A

A

T

S

A

S
|
S

G
|
G

M
x
H

S

A

A

A

I
x
Q

Y

F

L

L

A

A

V

L

M

T

N

T

M

L

V

A

E

Q

S

A

G

G

D

D

E

N

I

I

V

V

S

S

S

T

S

P

G

N

L

L

Y
|
Y

G

G

G

G

T

T

Y

F

T

N

L

Q

M

F

R

K

-

V

N

T

L

L

K

K

S

R

I

L

G

G

V

I

K

E

V

V

H

R

F

F

L

T

-

S

E

R

N

E

I

E

N

R

P

P

D

E

T

E

L

F

K

L

S

A

V

L

I

T

N

D

K

E

K

K

T

T

K

R

L

A

V

W

F

W

A

V

E

E

T

S

I

I

G

G

N

N

P

P

R

A

L

L

D

N

V

I

L

P

D

D

I

L

G

E

A

A

V

L

S

A

K

Q

F

A

C

A

K

R

D

E

N

K

N

G

I

V

V

A

L

L

I

I

I

V

D
|
D

S

N

T
|
T

M

F

T

G

T

M

P

G

-

G

Y

Y

L

L

I

L

K

R

P

P

L

L

E

A

Y

W

G

G

A

A

D

A

I

L

V

V

I

T

H

H

S
|
S

T

L

S
x
T

K
|
K

Y

W

I

V

N

G

G

G

T

H

S

G

N

A

S

V

I

I

G

A

G

G

I

A

I

I

V

V

D

D

G

G

G

G

S

N

S

F

K

P

Y

W

T

E

D

G

P

G

R

R

Y

Y

E

P

-

L

-

L

-

T

-

E

-

P

-

Q

-

P

-

G

-

Y

N

H

F

G

L

L

P

R

Y

L

T

T

K

E

K

A

Y

F

G

G

K

E

M

L

A

A

F

F

T

I

A

V

K

K

L

A

K

R

D

V

T

D

I

G

G

L

R

R

D

D

V

Q

G

G

P

Q

A

A

L

L

S

G

P

P

F

F

N

E

A

A

F

W

L

V

N

V

L

L

T

L

G

G

V

M

E

E

T

T

L

L

S

S

L

L

R

R

M

A

K

E

E

R

H

H

C

V

K

E

N

N

A

T

L

L

G

H

I

L

A

A

K

H

Y

W

L

L

S

L

N

E

N

Q

D

P

K

Q

I

V

V

A

D

W

V

V

N

N

Y

Y

P

P

G

G

L

L

E

P

S

H

S

H

R

P

Y

H

Y

H

D

D

L

R

A

A

Q

Q

K

K

Y

Y

Y

F

K

K

N

G

G

K

S

P

G

G

G

A

I

V

L

L

T

T

F

F

R

G

L

L

-

K

G

G

S

G

K

Y

E

E

N

A

A

A

F

K

K

R

F

F

L

I

N

S

N

R

L

L

K

K

L

L

I

I

L

S

D

H

L

L

T

A

N

N

I

V

G

G

D

D

T

T

K

R

S

T

I

L

I

A

I

I

H

H

P

P

S

A

S

S

T

T

I

T

C

H

S

S

N

Q

N

L

T

S

D

P

E

E

E

E

K

Q

R

A

Q

Q

M

A

G

G

V

V

Y

S

E

P

D

E

L

M

L

V

R

R

L

L

S

S

V

V

G

G

I

L

E

E

D

H

V

V

E

E

D

D

I

L

I

K

A

A

D

E

I

L

D

K

S

E

A

A

L

L

active site: R54 (= R53), Y107 (= Y106), D180 (= D177), K206 (= K202)
binding pyridoxal-5'-phosphate: S81 (= S80), G82 (= G81), H83 (≠ M82), Q86 (≠ I85), Y107 (= Y106), D180 (= D177), T182 (= T179), S203 (= S199), T205 (≠ S201), K206 (= K202)

Q5SK88 O-acetyl-L-homoserine sulfhydrylase 1; OAH-sulfhydrylase 1; EC 2.5.1.- from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
38% identity, 99% coverage: 1:406/409 of query aligns to 1:420/421 of Q5SK88

query
sites
Q5SK88

M

M

K

R

F

F

N

E

T

T

K

L

L

Q

I

L

H

H

G

A

N

G

A

Y

R

E

L

P

D

E

G

P

T

T

G

T

-

L

A

S

T

R

N

Q

T

V

P

P

V

I

Y

Y

L

P

S

T

N

T

A

S

Y

Y

A

V

H

F

T

K

S

S

A

P

E

E

K

H

L

A

E

A

K

N

I

L

F

F

Q

A

G

L

K

K

D

E

M

F

G

G

Y

N

A

I

Y

Y

T

S

R

R

I

I

S

M

N

N

P

P

T

T

V

V

T

D

S

V

F

L

E

E

R

K

R

R

I

L

A

A

S

A

I

L

E

E

N

G

G

G

M

K

V

A

A

A

T

L

S

A

A

T

S

A

S

S

G

G

M

H

S

A

A

A

I

Q

Y

F

L

L

A

A

V

L

M

T

N

T

M

L

V

A

E

Q

S

A

G

G

D

D

E

N

I

I

V

V

S

S

S

T

S

P

G

N

L

L

Y

Y

G

G

G

G

T

T

Y

F

T

N

L

Q

M

F

R

K

-

V

N

T

L

L

K

K

S

R

I

L

G

G

V

I

K

E

V

V

H

R

F

F

L

T

-

S

E

R

N

E

I

E

N

R

P

P

D

E

T

E

L

F

K

L

S

A

V

L

I

T

N

D

K

E

K

K

T

T

K

R

L

A

V

W

F

W

A

V

E

E

T

S

I

I

G

G

N

N

P

P

R

A

L

L

D

N

V

I

L

P

D

D

I

L

G

E

A

A

V

L

S

A

K

Q

F

A

C

A

K

R

D

E

N

K

N

G

I

V

V

A

L

L

I

I

I

V

D

D

S

N

T

T

M

F

T

G

T

M

P

G

-

G

Y

Y

L

L

I

L

K

R

P

P

L

L

E

A

Y

W

G

G

A

A

D

A

I

L

V

V

I

T

H

H

S

S

T

L

S

T

K
|
K

Y

W

I

V

N

G

G

G

T

H

S

G

N

A

S

V

I

I

G

A

G

G

I

A

I

I

V

V

D

D

G

G

G

G

S

N

S

F

K

P

Y

W

T

E

D

G

P

G

R

R

Y

Y

E

P

-

L

-

L

-

T

-

E

-

P

-

Q

-

P

-

G

-

Y

N

H

F

G

L

L

P

R

Y

L

T

T

K

E

K

A

Y

F

G

G

K

E

M

L

A

A

F

F

T

I

A

V

K

K

L

A

K

R

D

V

T

D

I

G

G

L

R

R

D

D

V

Q

G

G

P

Q

A

A

L

L

S

G

P

P

F

F

N

E

A

A

F

W

L

V

N

V

L

L

T

L

G

G

V

M

E

E

T

T

L

L

S

S

L

L

R

R

M

A

K

E

E

R

H

H

C

V

K

E

N

N

A

T

L

L

G

H

I

L

A

A

K

H

Y

W

L

L

S

L

N

E

N

Q

D

P

K

Q

I

V

V

A

D

W

V

V

N

N

Y

Y

P

P

G

G

L

L

E

P

S

H

S

H

R

P

Y

H

Y

H

D

D

L

R

A

A

Q

Q

K

K

Y

Y

Y

F

K

K

N

G

G

K

S

P

G

G

G

A

I

V

L

L

T

T

F

F

R

G

L

L

-

K

G

G

S

G

K

Y

E

E

N

A

A

A

F

K

K

R

F

F

L

I

N

S

N

R

L

L

K

K

L

L

I

I

L

S

D

H

L

L

T

A

N

N

I

V

G

G

D

D

T

T

K

R

S

T

I

L

I

A

I

I

H

H

P

P

S

A

S

S

T

T

I

T

C

H

S

S

N

Q

N

L

T

S

D

P

E

E

E

E

K

Q

R

A

Q

Q

M

A

G

G

V

V

Y

S

E

P

D

E

L

M

L

V

R

R

L

L

S

S

V

V

G

G

I

L

E

E

D

H

V

V

E

E

D

D

I

L

I

K

A

A

D

E

I

L

D

K

S

E

A

A

L

L

K206 (= K202) modified: N6-(pyridoxal phosphate)lysine

4hf8A Crystal structure of l-methionine gamma-lyase from citrobacter freundii with glycine (see paper)
37% identity, 99% coverage: 3:408/409 of query aligns to 8:394/396 of 4hf8A

query
sites
4hf8A

F

F

N

N

T

T

K

Q

L

I

I

V

H

H

G

A

N

G

A

Q

R

Q

L

P

D

D

-

P

G

S

T

T

G

G

A

A

T

L

N

S

T

T

P

P

V

I

Y

F

L

Q

S

T

N

S

A

T

Y

F

A

V

H

F

T

D

S

S

A

A

E

E

K

Q

L

G

E

A

K

A

I

R

F

F

Q

A

G

L

K

E

D

E

M

S

G

G

Y

Y

A

I

Y

Y

T

T

R
|
R

I

L

S

G

N

N

P

P

T

T

V

T

T

D

S

A

F

L

E

E

R

K

R

K

I

L

A

A

S

V

I

L

E

E

N

R

G

G

M

E

V

A

A

G

T

L

S

A

A

T

S

A

S

S

G
|
G

M
x
I

S

S

A

A

I

I

Y

T

L

T

A

T

V

L

M

L

N

T

M

L

V

C

E

Q

S

Q

G

G

D

D

E

H

I

I

V

V

S

S

S

A

S

S

G

A

L

I

Y
|
Y

G

G

G

C

T

T

Y

H

T

A

L

F

M

L

-

S

R

H

N

S

L

M

K

P

S

K

I

F

G

G

V

I

K

N

V

V

H

R

F

F

L

V

E

D

N

A

I

A

N

K

P

P

D

E

T

E

L

I

K

R

S

A

V

A

I

M

N

R

K

P

K

E

T

T

K

K

L

V

V

V

F

Y

A

I

E
|
E

T

T

I

P

G

A

N
|
N

P

P

R

T

L

L

D

S

V

L

L

V

D

D

I

I

G

E

A

T

V

V

S

A

K

G

F

I

C

A

K

H

D

Q

N

Q

N

G

I

A

V

L

L

L

I

V

I

V

D
|
D

S

N

T

T

M

F

T

M

T

S

P

P

Y

Y

L

C

I

Q

K

Q

P

P

L

L

E

Q

Y

L

G

G

A

A

D

D

I

I

V

V

I

V

H

H

S
|
S

T

V

S

T

K
|
K

Y

Y

I

I

N

N

G

G

T

H

S

G

N

D

S

V

I

I

G

G

G

G

I

I

I

I

V

V

D

-

G

-

G

G

S

K

S

Q

K

E

Y

F

T

I

D

D

P

-

R

-

Y

-

E

-

N

-

F

-

L

-

P

-

Y

-

T

-

K

-

K

-

Y

-

G

-

K

-

M

Q

A

A

F

R

T

F

A

V

K

G

L

L

K

K

D

D

T

I

I

-

G

-

R

-

D

-

V

T

G

G

P

G

A

C

L

M

S

S

P

P

F

F

N

N

A

A

F

W

L

L

N

T

L

L

T

R

G

G

V

V

E

K

T

T

L

L

S

G

L

I

R

R

M

M

K

E

E

R

H

H

C

C

K

E

N

N

A

A

L

L

G

K

I

I

A

A

K

R

Y

F

L

L

S

E

N

G

N

H

D

P

K

S

I

I

V

T

D

R

V

V

N

Y

Y

Y

P

P

G

G

L

L

E

S

S

S

S

H

R

P

Y

Q

Y

Y

D

E

L

L

A

G

Q

Q

K

R

Y

Q

Y

M

K

S

N

L

G

-

S

P

G

G

G

G

I

I

L

I

T

S

F

F

R

E

L

I

-

A

G

G

S

G

K

L

E

E

N

A

A

G

F

R

K

R

F

M

L

I

N

N

N

S

L

V

K

E

L

L

I

C

L

L

D

L

L

A

T

V

N
x
S

I

L

G

G

D

D

T

T

K

E

S

T

I

L

I

I

I

Q

H

H

P

P

S

A

S

S

T

M

I

T

C

H

S

S

N

P

N

V

T

A

D

P

E

E

E

E

K

R

R

L

Q

K

M

A

G

G

V

I

Y

T

E

D

D

G

L

L

L

I

R
|
R

L

L

S

S

V

V

G

G

I

L

E

E

D

D

V

P

E

E

D

D

I

I

I

I

A

N

D

D

I

L

D

E

S

H

A

A

L

I

K

R

E

K

active site: R59 (= R53), Y112 (= Y106), D184 (= D177), K209 (= K202)
binding n-glycine-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-yl-methane]: G87 (= G81), I88 (≠ M82), Y112 (= Y106), E155 (= E148), N159 (= N152), D184 (= D177), S206 (= S199), K209 (= K202), S338 (≠ N352), R373 (= R387)

O13326 Homocysteine synthase; O-acetylhomoserine sulfhydrylase; OAH SHL; OAH sulfhydrylase; EC 2.5.1.49 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
39% identity, 99% coverage: 3:407/409 of query aligns to 8:427/429 of O13326

query
sites
O13326

F

F

N

E

T

T

K

L

L

Q

I

L

H

H

G

A

N

G

A

Q

R

E

L

P

D

D

G

A

-

A

T

T

G

S

A

S

T

R

N

A

T

V

P

P

V

I

Y

Y

L

A

S

T

N

T

A

S

Y

Y

A

V

H

F

T

R

S

D

A

C

E

D

K

H

L

G

E

G

K

R

I

L

F

F

Q

G

G

L

K

Q

D

E

M

P

G

G

Y

Y

A

I

Y

Y

T

S

R

R

I

M

S

M

N

N

P

P

T

T

V

A

T

D

S

V

F

F

E

E

R

K

R

R

I

I

A

A

S

A

I

L

E

E

N

H

G

G

M

A

V

A

A

A

T

I

S

A

A

T

S

S

S

S

G

G

M

T

S

S

A

A

I

L

Y

F

L

M

A

A

V

L

M

T

N

T

M

L

V

A

E

K

S

A

G

G

D

D

E

N

I

I

V

V

S

S

S

T

S

S

G

Y

L

L

Y

Y

G

G

G

G

T

T

Y

Y

T

N

L

L

M

F

R

K

-

V

N

T

L

L

K

P

S

R

I

L

G

G

V

I

K

T

V

T

H

K

F

F

L

V

E

N

N

G

I

D

N

D

P

P

D

N

T

D

L

L

K

A

S

A

V

Q

I

I

N

D

K

E

K

N

T

T

K

K

L

A

V

V

F

Y

A

V

E

E

T

S

I

I

G

G

N

N

P

P

R

M

L

Y

D

N

V

V

L

P

D

D

I

F

G

E

A

R

V

I

S

A

K

E

F

V

C

A

K

H

D

A

N

A

N

G

I

V

V

P

L

L

I

M

I

V

D

D

S

N

T

T

M

F

T

G

T

G

P

G

-

G

Y

Y

L

L

I

V

K

R

P

P

L

I

E

D

Y

H

G

G

A

A

D

D

I

I

V

V

I

T

H

H

S

S

T

A

S

T

K

K

Y

W

I

I

N

G

G

G

T

H

S

G

N

T

S

T

I

I

G

G

G

G

I

V

I

I

V

V

D

D

G

S

G

G

S

K

-

F

-

D

S

W

K

K

Y

K

T

N

D

S

P

K

R

R

Y

F

E

P

N

E

F

F

-

N

-

E

-

P

-

H

-

P

-

G

-

Y

-

H

-

G

L

M

P

V

Y

F

T

T

K

E

K

T

Y

F

G

G

K

N

M

L

A

A

F

Y

T

A

A

F

K

A

L

C

K

R

D

T

T

Q

I

T

G

L

R

R

D

D

V

V

G

G

P

G

A

N

L

A

S

N

P

P

F

F

N

G

A

V

F

F

L

L

N

L

L

L

T

Q

G

G

V

L

E

E

T

T

L

L

S

S

L

L

R

R

M

M

K

E

E

R

H

H

C

V

K

Q

N

N

A

A

L

F

G

A

I

L

A

A

K

K

Y

Y

L

L

S

E

N

K

N

H

D

P

K

K

I

V

V

N

D

W

V

V

N

S

Y

Y

P

P

G

G

L

L

E

E

S

S

S

H

R

V

Y

S

Y

H

D

K

L

L

A

A

Q

K

K

K

Y

Y

Y

L

K

K

N

N

G

G

S

Y

G

G

G

A

I

V

L

L

T

S

F

F

R

G

L

A

-

K

G

G

S

G

K

P

E

D

N

Q

A

S

F

R

K

K

F

V

L

V

N

N

N

A

L

L

K

K

L

L

I

A

L

S

D

Q

L

L

T

A

N

N

I

V

G

G

D

D

T

A

K

K

S

T

I

L

I

V

I

I

H

A

P

P

S

A

S

Y

T

T

I

T

C

H

S

L

N

Q

N

L

T

T

D

D

E

E

E

E

K

Q

R

I

Q

S

M

A

G

G

V

V

Y

T

E

K

D

D

L

L

L

I

R

R

L

V

S

A

V

V

G
|
G

I

I

E

E

D

H

V

I

E

D

D

D

I

I

I

I

A

A

D

D

I

F

D

A

S

Q

A

A

L

L

K

E

G411 (= G391) mutation to D: Impairs homocysteine synthase activity.

4omaA The crystal structure of methionine gamma-lyase from citrobacter freundii in complex with l-cycloserine pyridoxal-5'-phosphate (see paper)
37% identity, 99% coverage: 3:408/409 of query aligns to 8:394/396 of 4omaA

query
sites
4omaA

F

F

N

N

T

T

K

Q

L

I

I

V

H

H

G

A

N

G

A

Q

R

Q

L

P

D

D

-

P

G

S

T

T

G

G

A

A

T

L

N

S

T

T

P

P

V

I

Y

F

L

Q

S

T

N

S

A

T

Y

F

A

V

H

F

T

D

S

S

A

A

E

E

K

Q

L

G

E

A

K

A

I

R

F

F

Q

A

G

L

K

E

D

E

M

S

G

G

Y

Y

A

I

Y

Y

T

T

R
|
R

I

L

S

G

N

N

P

P

T

T

V

T

T

D

S

A

F

L

E

E

R

K

R

K

I

L

A

A

S

V

I

L

E

E

N

R

G

G

M

E

V

A

A

G

T

L

S

A

A

T

S

A

S

S

G
|
G

M
x
I

S

S

A

A

I

I

Y

T

L

T

A

T

V

L

M

L

N

T

M

L

V

C

E

Q

S

Q

G

G

D

D

E

H

I

I

V

V

S

S

S

A

S

S

G

A

L

I

Y
|
Y

G

G

G

C

T

T

Y

H

T

A

L

F

M

L

-

S

R

H

N

S

L

M

K

P

S

K

I

F

G

G

V

I

K

N

V

V

H

S

F

F

L

V

E

D

N

A

I

A

N

K

P

P

D

E

T

E

L

I

K

R

S

A

V

A

I

M

N

R

K

P

K

E

T

T

K

K

L

V

V

V

F

Y

A

I

E

E

T

T

I

P

G

A

N

N

P

P

R

T

L

L

D

S

V

L

L

V

D

D

I

I

G

E

A

T

V

V

S

A

K

G

F

I

C

A

K

H

D

Q

N

Q

N

G

I

A

V

L

L

L

I

V

I

V

D
|
D

S

N

T

T

M

F

T

M

T

S

P

P

Y

Y

L

C

I

Q

K

Q

P

P

L

L

E

Q

Y

L

G

G

A

A

D

D

I

I

V

V

I

V

H

H

S
|
S

T

V

S
x
T

K
|
K

Y

Y

I

I

N

N

G

G

T

H

S

G

N

D

S

V

I

I

G

G

G

G

I

I

I

I

V

V

D

-

G

-

G

G

S

K

S

Q

K

E

Y

F

T

I

D

D

P

-

R

-

Y

-

E

-

N

-

F

-

L

-

P

-

Y

-

T

-

K

-

K

-

Y

-

G

-

K

-

M

Q

A

A

F

R

T

F

A

V

K

G

L

L

K

K

D

D

T

I

I

T

G

G

R

G

D

-

V

-

G

-

P

-

A

C

L

M

S

S

P

P

F

F

N

N

A

A

F

W

L

L

N

T

L

L

T

R

G

G

V

V

E

K

T

T

L

L

S

G

L

I

R

R

M

M

K

E

E

R

H

H

C

C

K

E

N

N

A

A

L

L

G

K

I

I

A

A

K

R

Y

F

L

L

S

E

N

G

N

H

D

P

K

S

I

I

V

T

D

R

V

V

N

Y

Y

Y

P

P

G

G

L

L

E

S

S

S

S

H

R

P

Y

Q

Y

Y

D

E

L

L

A

G

Q

Q

K

R

Y

Q

Y

M

K

S

N

L

G

-

S

P

G

G

G

G

I

I

L

I

T

S

F

F

R

E

L

I

-

A

G

G

S

G

K

L

E

E

N

A

A

G

F

R

K

R

F

M

L

I

N

N

N

S

L

V

K

E

L

L

I

C

L

L

D

L

L

A

T
x
V

N
x
S

I

L

G

G

D

D

T

T

K

E

S

T

I

L

I

I

I

Q

H

H

P

P

S

A

S

S

T

M

I

T

C

H

S

S

N

P

N

V

T

A

D

P

E

E

E

E

K

R

R

L

Q

K

M

A

G

G

V

I

Y

T

E

D

D

G

L

L

L

I

R
|
R

L

L

S

S

V

V

G

G

I

L

E

E

D

D

V

P

E

E

D

D

I

I

I

I

A

N

D

D

I

L

D

E

S

H

A

A

L

I

K

R

E

K

active site: R59 (= R53), Y112 (= Y106), D184 (= D177), K209 (= K202)
binding [5-hydroxy-6-methyl-4-({[(4E)-3-oxo-1,2-oxazolidin-4-ylidene]amino}methyl)pyridin-3-yl]methyl dihydrogen phosphate: G87 (= G81), I88 (≠ M82), Y112 (= Y106), D184 (= D177), S206 (= S199), T208 (≠ S201), K209 (= K202), V337 (≠ T351), S338 (≠ N352), R373 (= R387)

3jwbA Crystal structure of l-methionine gamma-lyase from citrobacter freundii with norleucine (see paper)
37% identity, 99% coverage: 3:408/409 of query aligns to 8:394/396 of 3jwbA

query
sites
3jwbA

F

F

N

N

T

T

K

Q

L

I

I

V

H

H

G

A

N

G

A

Q

R

Q

L

P

D

D

-

P

G

S

T

T

G

G

A

A

T

L

N

S

T

T

P

P

V

I

Y

F

L

Q

S

T

N

S

A

T

Y

F

A

V

H

F

T

D

S

S

A

A

E

E

K

Q

L

G

E

A

K

A

I

R

F

F

Q

A

G

L

K

E

D

E

M

S

G

G

Y

Y

A

I

Y

Y

T

T

R
|
R

I

L

S

G

N

N

P

P

T

T

V

T

T

D

S

A

F

L

E

E

R

K

R

K

I

L

A

A

S

V

I

L

E

E

N

R

G

G

M

E

V

A

A

G

T

L

S

A

A

T

S

A

S

S

G

G

M

I

S

S

A

A

I

I

Y

T

L

T

A

T

V

L

M

L

N

T

M

L

V

C

E

Q

S

Q

G

G

D

D

E

H

I

I

V

V

S

S

S

A

S

S

G

A

L

I

Y
|
Y

G

G

G

C

T

T

Y

H

T

A

L

F

M

L

-

S

R

H

N

S

L

M

K

P

S

K

I

F

G

G

V

I

K

N

V

V

H

S

F

F

L

V

E

D

N

A

I

A

N

K

P

P

D

E

T

E

L

I

K

R

S

A

V

A

I

M

N

R

K

P

K

E

T

T

K

K

L

V

V

V

F

Y

A

I

E

E

T

T

I

P

G

A

N

N

P

P

R

T

L

L

D

S

V

L

L

V

D

D

I

I

G

E

A

T

V

V

S

A

K

G

F

I

C

A

K

H

D

Q

N

Q

N

G

I

A

V

L

L

L

I

V

I

V

D
|
D

S

N

T

T

M

F

T

M

T

S

P

P

Y

Y

L

C

I

Q

K

Q

P

P

L

L

E

Q

Y

L

G

G

A

A

D

D

I

I

V

V

I

V

H

H

S

S

T

V

S

T

K
|
K

Y

Y

I

I

N

N

G

G

T

H

S

G

N

D

S

V

I

I

G

G

G

G

I

I

I

I

V

V

D

-

G

-

G

G

S

K

S

Q

K

E

Y

F

T

I

D

D

P

-

R

-

Y

-

E

-

N

-

F

-

L

-

P

-

Y

-

T

-

K

-

K

-

Y

-

G

-

K

-

M

Q

A

A

F

R

T

F

A

V

K

G

L

L

K

K

D

D

T

I

I

T

G

G

R

G

D

-

V

-

G

-

P

-

A

C

L

M

S

S

P

P

F

F

N

N

A

A

F

W

L

L

N

T

L

L

T

R

G

G

V

V

E

K

T

T

L

L

S

G

L

I

R

R

M

M

K

E

E

R

H

H

C

C

K

E

N

N

A

A

L

L

G

K

I

I

A

A

K

R

Y

F

L

L

S

E

N

G

N

H

D

P

K

S

I

I

V

T

D

R

V

V

N

Y

Y

Y

P

P

G

G

L

L

E

S

S

S

S

H

R

P

Y

Q

Y

Y

D

E

L

L

A

G

Q

Q

K

R

Y

Q

Y

M

K

S

N

L

G

-

S

P

G

G

G

G

I

I

L

I

T

S

F

F

R

E

L

I

-

A

G

G

S

G

K

L

E

E

N

A

A

G

F

R

K

R

F

M

L

I

N

N

N

S

L

V

K

E

L

L

I

C

L

L

D

L

L

A

T

V

N
x
S

I

L

G

G

D

D

T

T

K

E

S

T

I

L

I

I

I

Q

H

H

P

P

S

A

S

S

T

M

I

T

C

H

S

S

N

P

N

V

T

A

D

P

E

E

E

E

K

R

R

L

Q

K

M

A

G

G

V

I

Y

T

E

D

D

G

L

L

L

I

R
|
R

L

L

S

S

V

V

G

G

I

L

E

E

D

D

V

P

E

E

D

D

I

I

I

I

A

N

D

D

I

L

D

E

S

H

A

A

L

I

K

R

E

K

active site: R59 (= R53), Y112 (= Y106), D184 (= D177), K209 (= K202)
binding norleucine: Y112 (= Y106), K209 (= K202), S338 (≠ N352), R373 (= R387)

3jwaA Crystal structure of l-methionine gamma-lyase from citrobacter freundii with methionine phosphinate (see paper)
37% identity, 99% coverage: 3:408/409 of query aligns to 8:394/396 of 3jwaA

query
sites
3jwaA

F

F

N

N

T

T

K

Q

L

I

I

V

H

H

G

A

N

G

A

Q

R

Q

L

P

D

D

-

P

G

S

T

T

G

G

A

A

T

L

N

S

T

T

P

P

V

I

Y

F

L

Q

S

T

N

S

A

T

Y

F

A

V

H

F

T

D

S

S

A

A

E

E

K

Q

L

G

E

A

K

A

I

R

F

F

Q

A

G

L

K

E

D

E

M

S

G

G

Y

Y

A

I

Y

Y

T

T

R
|
R

I

L

S

G

N

N

P

P

T

T

V

T

T

D

S

A

F

L

E

E

R

K

R

K

I

L

A

A

S

V

I

L

E

E

N

R

G

G

M

E

V

A

A

G

T

L

S

A

A

T

S

A

S

S

G

G

M

I

S

S

A

A

I

I

Y

T

L

T

A

T

V

L

M

L

N

T

M

L

V

C

E

Q

S

Q

G

G

D

D

E

H

I

I

V

V

S

S

S

A

S

S

G

A

L

I

Y
|
Y

G

G

G

C

T

T

Y

H

T

A

L

F

M

L

-

S

R

H

N

S

L

M

K

P

S

K

I

F

G

G

V

I

K

N

V

V

H

S

F

F

L

V

E

D

N

A

I

A

N

K

P

P

D

E

T

E

L

I

K

R

S

A

V

A

I

M

N

R

K

P

K

E

T

T

K

K

L

V

V

V

F

Y

A

I

E

E

T

T

I

P

G

A

N

N

P

P

R

T

L

L

D

S

V

L

L

V

D

D

I

I

G

E

A

T

V

V

S

A

K

G

F

I

C

A

K

H

D

Q

N

Q

N

G

I

A

V

L

L

L

I

V

I

V

D
|
D

S

N

T

T

M

F

T

M

T

S

P

P

Y

Y

L

C

I

Q

K

Q

P

P

L

L

E

Q

Y

L

G

G

A

A

D

D

I

I

V

V

I

V

H

H

S

S

T

V

S

T

K
|
K

Y

Y

I

I

N

N

G

G

T

H

S

G

N

D

S

V

I

I

G

G

G

G

I

I

I

I

V

V

D

-

G

-

G

G

S

K

S

Q

K

E

Y

F

T

I

D

D

P

-

R

-

Y

-

E

-

N

-

F

-

L

-

P

-

Y

-

T

-

K

-

K

-

Y

-

G

-

K

-

M

Q

A

A

F

R

T

F

A

V

K

G

L

L

K

K

D

D

T

I

I

T

G

G

R

G

D

-

V

-

G

-

P

-

A

C

L

M

S

S

P

P

F

F

N

N

A

A

F

W

L

L

N

T

L

L

T

R

G

G

V

V

E

K

T

T

L

L

S

G

L

I

R

R

M

M

K

E

E

R

H

H

C

C

K

E

N

N

A

A

L

L

G

K

I

I

A

A

K

R

Y

F

L

L

S

E

N

G

N

H

D

P

K

S

I

I

V

T

D

R

V

V

N

Y

Y

Y

P

P

G

G

L

L

E

S

S

S

S

H

R

P

Y

Q

Y

Y

D

E

L

L

A

G

Q

Q

K

R

Y

Q

Y

M

K

S

N

L

G

-

S

P

G

G

G

G

I

I

L

I

T

S

F

F

R

E

L

I

-

A

G

G

S

G

K

L

E

E

N

A

A

G

F

R

K

R

F

M

L

I

N

N

N

S

L

V

K

E

L

L

I

C

L

L

D

L

L

A

T
x
V

N
x
S

I

L

G

G

D

D

T

T

K

E

S

T

I

L

I

I

I

Q

H

H

P

P

S

A

S

S

T

M

I

T

C

H

S

S

N

P

N

V

T

A

D

P

E

E

E

E

K

R

R

L

Q

K

M

A

G

G

V

I

Y

T

E

D

D

G

L

L

L

I

R
|
R

L

L

S

S

V

V

G

G

I

L

E

E

D

D

V

P

E

E

D

D

I

I

I

I

A

N

D

D

I

L

D

E

S

H

A

A

L

I

K

R

E

K

active site: R59 (= R53), Y112 (= Y106), D184 (= D177), K209 (= K202)
binding (1-amino-3-methylsulfanyl-propyl)-phosphinic acid: Y112 (= Y106), K209 (= K202), V337 (≠ T351), S338 (≠ N352), R373 (= R387)

3jw9A Crystal structure of l-methionine gamma-lyase from citrobacter freundii with s-ethyl-cysteine (see paper)
37% identity, 99% coverage: 3:408/409 of query aligns to 8:394/396 of 3jw9A

query
sites
3jw9A

F

F

N

N

T

T

K

Q

L

I

I

V

H

H

G

A

N

G

A

Q

R

Q

L

P

D

D

-

P

G

S

T

T

G

G

A

A

T

L

N

S

T

T

P

P

V

I

Y

F

L

Q

S

T

N

S

A

T

Y

F

A

V

H

F

T

D

S

S

A

A

E

E

K

Q

L

G

E

A

K

A

I

R

F

F

Q

A

G

L

K

E

D

E

M

S

G

G

Y

Y

A

I

Y

Y

T

T

R
|
R

I

L

S

G

N

N

P

P

T

T

V

T

T

D

S

A

F

L

E

E

R

K

R

K

I

L

A

A

S

V

I

L

E

E

N

R

G

G

M

E

V

A

A

G

T

L

S

A

A

T

S

A

S

S

G

G

M

I

S

S

A

A

I

I

Y

T

L

T

A

T

V

L

M

L

N

T

M

L

V

C

E

Q

S

Q

G

G

D

D

E

H

I

I

V

V

S

S

S

A

S

S

G

A

L

I

Y
|
Y

G

G

G

C

T

T

Y

H

T

A

L

F

M

L

-

S

R

H

N

S

L

M

K

P

S

K

I

F

G

G

V

I

K

N

V

V

H

S

F

F

L

V

E

D

N

A

I

A

N

K

P

P

D

E

T

E

L

I

K

R

S

A

V

A

I

M

N

R

K

P

K

E

T

T

K

K

L

V

V

V

F

Y

A

I

E

E

T

T

I

P

G

A

N

N

P

P

R

T

L

L

D

S

V

L

L

V

D

D

I

I

G

E

A

T

V

V

S

A

K

G

F

I

C

A

K

H

D

Q

N

Q

N

G

I

A

V

L

L

L

I

V

I

V

D
|
D

S

N

T

T

M

F

T

M

T

S

P

P

Y

Y

L

C

I

Q

K

Q

P

P

L

L

E

Q

Y

L

G

G

A

A

D

D

I

I

V

V

I

V

H

H

S

S

T

V

S

T

K
|
K

Y

Y

I

I

N

N

G

G

T

H

S

G

N

D

S

V

I

I

G

G

G

G

I

I

I

I

V

V

D

-

G

-

G

G

S

K

S

Q

K

E

Y

F

T

I

D

D

P

-

R

-

Y

-

E

-

N

-

F

-

L

-

P

-

Y

-

T

-

K

-

K

-

Y

-

G

-

K

-

M

Q

A

A

F

R

T

F

A

V

K

G

L

L

K

K

D

D

T

I

I

T

G

G

R

G

D

-

V

-

G

-

P

-

A

C

L

M

S

S

P

P

F

F

N

N

A

A

F

W

L

L

N

T

L

L

T

R

G

G

V

V

E

K

T

T

L

L

S

G

L

I

R

R

M

M

K

E

E

R

H

H

C

C

K

E

N

N

A

A

L

L

G

K

I

I

A

A

K

R

Y

F

L

L

S

E

N

G

N

H

D

P

K

S

I

I

V

T

D

R

V

V

N

Y

Y

Y

P

P

G

G

L

L

E

S

S

S

S

H

R

P

Y

Q

Y

Y

D

E

L

L

A

G

Q

Q

K

R

Y

Q

Y

M

K

S

N

L

G

-

S

P

G

G

G

G

I

I

L

I

T

S

F

F

R

E

L

I

-

A

G

G

S

G

K

L

E

E

N

A

A

G

F

R

K

R

F

M

L

I

N

N

N

S

L

V

K

E

L

L

I

C

L

L

D

L

L

A

T
x
V

N
x
S

I

L

G

G

D

D

T

T

K

E

S

T

I

L

I

I

I

Q

H

H

P

P

S

A

S

S

T

M

I

T

C

H

S

S

N

P

N

V

T

A

D

P

E

E

E

E

K

R

R

L

Q

K

M

A

G

G

V

I

Y

T

E

D

D

G

L

L

L

I

R

R

L

L

S

S

V

V

G

G

I

L

E

E

D

D

V

P

E

E

D

D

I

I

I

I

A

N

D

D

I

L

D

E

S

H

A

A

L

I

K

R

E

K

active site: R59 (= R53), Y112 (= Y106), D184 (= D177), K209 (= K202)
binding S-ethyl-L-cysteine: Y112 (= Y106), K209 (= K202), V337 (≠ T351), S338 (≠ N352)

6egrA Crystal structure of citrobacter freundii methionine gamma-lyase with v358y replacement (see paper)
37% identity, 99% coverage: 3:408/409 of query aligns to 8:394/396 of 6egrA

query
sites
6egrA

F

F

N

N

T

T

K

Q

L

I

I

V

H

H

G

A

N

G

A

Q

R

Q

L

P

D

D

-

P

G

S

T

T

G

G

A

A

T

L

N

S

T

T

P

P

V

I

Y

F

L

Q

S

T

N

S

A

T

Y

F

A