SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_026127746.1 NCBI__GCF_000341125.1:WP_026127746.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P9WQ73 Phosphoserine aminotransferase; Phosphohydroxythreonine aminotransferase; PSAT; EC 2.6.1.52 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
66% identity, 100% coverage: 1:373/374 of query aligns to 5:376/376 of P9WQ73

query
sites
P9WQ73

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T154 (= T150) binding pyridoxal 5'-phosphate
D176 (= D173) binding pyridoxal 5'-phosphate
Q199 (= Q196) binding pyridoxal 5'-phosphate

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

2fyfA Structure of a putative phosphoserine aminotransferase from mycobacterium tuberculosis (see paper)
66% identity, 99% coverage: 5:373/374 of query aligns to 2:368/368 of 2fyfA

query
sites
2fyfA

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active site: F101 (= F104), D168 (= D173), K192 (= K197)
binding tetrachloroplatinate(ii): L2 (≠ I5), I321 (≠ V326), A324 (= A329)
binding pyridoxal-5'-phosphate: A77 (≠ T80), T78 (= T81), W81 (= W84), F101 (= F104), T147 (= T150), D168 (= D173), T170 (= T175), Q191 (= Q196), K192 (= K197), N243 (= N248), T244 (= T249)

Sites not aligning to the query:

binding tetrachloroplatinate(ii): 1

3ffrA Crystal structure of a phosphoserine aminotransferase serc (chu_0995) from cytophaga hutchinsonii atcc 33406 at 1.75 a resolution
26% identity, 93% coverage: 18:366/374 of query aligns to 7:356/361 of 3ffrA

query
sites
3ffrA

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binding serine: F93 (= F104), Y328 (= Y338)

5yb0B Crystal structure of wild type phosphoserine aminotransferase (psat) from e. Histolytica (see paper)
23% identity, 94% coverage: 17:368/374 of query aligns to 3:347/349 of 5yb0B

query
sites
5yb0B

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S

L

K

P

P

V

I

D

D

I

I

A

S

E

K

T

Y

D

S

V

L

Y

I

Y

Y

F

A

A

G

P

A

Q
|
Q

K
|
K

S

N

F

C

A

G

A

A

D

-

G

A

G

G

L

V

W

T

I

I

A

V

V

I

M

I

S

K

P

K

R

E

A

I

L

L

A

G

R

K

V

V

E

Q

E

-

I

-

A

-

G

-

R

R

Y

K

I

I

P

P

E

I

F

I

F

L

S

D

L

Y

T

Q

T

V

A

H

V

I

A

L

N

N

S

-

R

-

K

-

D

N

Q

S

T

M

Y

Y

N
|
N

T
|
T

P

P

A

P

V

V

A

I

T

S

L

I

L

F

L

T

L

V

A

N

E

Q

Q

T

L

L

E

K

W

Y

M

I

N

K

T

K

R

I

G

G

L

L

E

K

W

K

T

I

V

Q

A

E

R

L

T

N

A

E

E

E

S

K

S

A

S

R

I

L

L

L

Y

Y

D

A

W

E

A

I

E

D

K

R

S

N

P

K

V

I

A

-

S

-

P

-

F

F

V

R

T

G

D

T

P

V

A

R

K

K

R

K

S

D

Q

R

V

S

V

I

G

M

T

N

I

V

D

C

F

F

-

V

-

M

-

E

-

Q

-

Y

-

K

-

Q

-

L

S

E

D

N

D

E

V

F

D

S

A

E

A

Y

A

A

I

L

A

Q

K

K

V

-

L

-

R

-

A

-

N

-

G

G

V

I

D

G

T

I

E

K

P

G

Y

H

R

R

K

S

L

V

G

G

R

-

N

-

Q

G

L

F

R

R

V

A

A

S

M

I

F

Y

P

N

A

A

V

V

E

T

P

I

G

E

D

S

V

V

H

Q

K

A

L

L

T

I

E

K

C

C

V

M

-

H

D

D

F

F

binding pyridoxal-5'-phosphate: A72 (≠ T80), S73 (≠ T81), W96 (≠ F104), T143 (= T150), D162 (≠ G163), S164 (≠ D165), Q185 (= Q196), K186 (= K197), N227 (= N248), T228 (= T249)

Q9Y617 Phosphoserine aminotransferase; Phosphohydroxythreonine aminotransferase; PSAT; EC 2.6.1.52 from Homo sapiens (Human) (see 6 papers)
23% identity, 93% coverage: 17:362/374 of query aligns to 9:357/370 of Q9Y617

query
sites
Q9Y617

R

N

F

F

G

G

S

P

G

G

P

P

S

A

K

K

V

L

R

P

P

H

E

S

Q

V

L

L

E

L

A

E

L

I

A

Q

S

K

S

E

G

L

A

L

R

D

Y

Y

M

K

G

G

T

V

-

G

-

I

-

S

-

V

-

L

-

E

-

M

S
|
S

H
|
H

R
|
R

Q

S

K

S

P

D

V

F

K

A

S

K

L

I

V

I

S

N

R

N

V

T

R

E

S

N

G

L

V

V

S

R

D

E

L

L

F

L

S

A

L

V

P

P

E

D

G

N

Y
|
Y

E

K

V

V

V

I

L

F

G

L

N

Q

G

G

T
x
G

T
x
C

A

G

F

Q

W

F

D

S

I

A

A

V

A
x
P

H

L

G

N

L

L

L

I

R

G

S

L

K

K

S

A

Q

G

H

R

L

C

A
|
A

-
x
D

-

Y

-

V

-

T

-

G

-

A

-
x
W

-

S

-

A

-

K

F

A

G

A

E

E

F

E

S

A

S

K

K

K

F

F

A

G

K

T

V

I

A

N

A

I

G

V

A

H

P

P

W

K

L

L

E

G

E

S

P

Y

T

T

V

K

V

I

S

P

T

-

D

D

P

P

G

-

S

S

H

T

G

W

E

N

A

L

V

N

A

P

E

D

A

A

G

S

V

Y

D

V

V

Y

Y

Y

A

C

L

-

T

-

H

A

N

N

E
|
E

T
|
T

S

V

T

H

G

G

V

V

A

E

A

F

P

D

-

F

I

I

R

P

R

D

V

V

A

K

G

G

A

A

D

-

A

-

L

V

V

L

L

V

V

C

D
|
D

A

M

T

S

S
|
S

G

N

A

F

G

L

G

S

L

K

P

P

V

V

D

D

I

V

A

S

E

K

T

F

D

G

V

V

Y

I

Y

F

F

A

A

G

P

A

Q
|
Q

K
|
K

S

N

F

V

A

G

A

S

D

A

G

G

G

V

L

T

W

V

I

V

A

I

V

V

M

-

S

-

P

-

R

-

A

-

L

-

A

-

R

R

V

D

E

D

E

L

I

L

A

G

A

F

G

A

G

L

R

R

Y

E

I

C

P

P

E

S

F

V

F

L

S

E

L

Y

T

K

T

V

A

Q

V

A

A

G

N

N

S

S

R

-

K

-

D

-

Q

S

T

L

Y

Y

N
|
N

T
|
T

P

P

A

P

V
x
C

A

F

T

S

L

I

L

Y

L

V

L

M

A

G

E

L

Q

V

L

L

E

E

W

W

M

I

N

K

T

N

R

N

G

G

L

A

E

A

W

A

T

M

V

E

A

K

R

L

T

S

A

S

E

I

S

K

S

S

S

Q

I

T

L

I

Y

Y

D

E

W

I

A

I

E

D

K

N

S

S

P

Q

V

G

A

F

S

Y

P

V

F

C

V

P

T

V

D

E

P

P

A

Q

K

N

R

R

S

S

Q

K

V

M

-

N

-

I

-

P

-

F

-

R

V

I

G

G

T

N

I

A

D

K

F

G

S

D

D

D

D

A

V

L

D

E

A

K

A

R

A

F

I

L

A

D

K

K

V

A

L

L

R

E

A

L

N

N

G

-

V

M

V

L

D

S

T

L

E

K

P

G

Y
x
H

R
|
R

K

S

L

V

G

G

R

-

N

-

Q

G

L

I

R
|
R

V

A

A

S

M

L

F

Y

P

N

A

A

V

V

E

T

P

I

G

E

D

D

V

V

H

Q

K

K

L

L

S43 (= S44) to R: in PSATD; reduced O-phospho-L-serine:2-oxoglutarate aminotransferase catalytic efficiency; 3-fold increase of KM for 3-phosphohydroxypyruvate; 5-fold increase of KM for L-glutamate; decreased function in L-serine biosynthesis shown through in vitro reconstruction of the phosphorylated pathway; does not affect secondary structure; does not affect dimerization; does not affect thermal stability
H44 (= H45) binding in other chain
R45 (= R46) binding in other chain
Y70 (= Y71) to N: in NLS2; uncertain significance
G79 (≠ T80) binding pyridoxal 5'-phosphate; to W: in NLS2; loss of O-phospho-L-serine:2-oxoglutarate aminotransferase activity; loss of function in L-serine biosynthesis shown through in vitro reconstruction of the phosphorylated pathway
C80 (≠ T81) binding pyridoxal 5'-phosphate
P87 (≠ A88) to A: has no effect on O-phospho-L-serine:2-oxoglutarate aminotransferase catalytic efficiency; does not affect KM for 3-phosphohydroxypyruvate; does not affect KM for L-glutamate; no effect on function in L-serine biosynthesis shown through in vitro reconstruction of the phosphorylated pathway; does not affect secondary structure; does not affect dimerization; does not affect thermal stability; dbSNP:rs11540974
A99 (= A100) to V: in NLS2; does not affect secondary structure; does not affect dimerization; increased thermal stability; dbSNP:rs587777778
D100 (vs. gap) to A: in PSATD; has no effect on O-phospho-L-serine:2-oxoglutarate aminotransferase catalytic efficiency; does not affect KM for 3-phosphohydroxypyruvate; does not affect KM for L-glutamate; does not affect secondary structure; results in increased protein aggregation as shown by dynamic light scattering; dbSNP:rs118203967
W107 (vs. gap) binding pyridoxal 5'-phosphate
E155 (= E149) to Q: in NLS2; uncertain significance
T156 (= T150) binding pyridoxal 5'-phosphate
D176 (= D173) binding pyridoxal 5'-phosphate
S179 (= S176) to L: in NLS2; loss of O-phospho-L-serine:2-oxoglutarate aminotransferase activity; loss of function in L-serine biosynthesis shown through in vitro reconstruction of the phosphorylated pathway; dbSNP:rs587777777
Q199 (= Q196) binding pyridoxal 5'-phosphate
K200 (= K197) modified: N6-(pyridoxal phosphate)lysine
N241 (= N248) binding in other chain
T242 (= T249) binding in other chain
C245 (≠ V252) to R: in NLS2; reduced O-phospho-L-serine:2-oxoglutarate aminotransferase catalytic efficiency; 9-fold increase of KM for L-glutamate; does not affect KM for 3-phosphohydroxypyruvate; decreased function in L-serine biosynthesis shown through in vitro reconstruction of the phosphorylated pathway; does not affect secondary structure; does not affect dimerization
H335 (≠ Y338) binding O-phospho-L-serine
R336 (= R339) binding O-phospho-L-serine
R342 (= R347) binding O-phospho-L-serine; to W: in NLS2; loss of O-phospho-L-serine:2-oxoglutarate aminotransferase activity; loss of function in L-serine biosynthesis shown through in vitro reconstruction of the phosphorylated pathway; dbSNP:rs202103028

1bt4A Phosphoserine aminotransferase from bacillus circulans subsp. Alkalophilus
23% identity, 90% coverage: 18:354/374 of query aligns to 7:341/361 of 1bt4A

query
sites
1bt4A

F

F

G

N

S

A

G

G

P

P

S

A

K

A

V

L

R

P

P

L

E

E

Q

V

L

L

E

E

A

R

-

A

-

Q

-

A

-

E

-

F

-

V

-

D

L

Y

A

Q

S

H

S

T

G

G

A

M

R

S

Y

I

M

M

G

E

T

M

S

S

H

H

R

R

Q

G

K

A

P

V

V

Y

K

E

S

A

L

V

V

H

S

N

R

E

V

A

R

Q

S

A

G

R

V

L

S

L

D

A

L

L

F

L

S

G

L

N

P

P

E

T

G

G

Y

Y

E

K

V

V

V

L

L

F

G

I

N

Q

G

G

T
x
A

T
x
S

A

T

F

Q

W

F

D

A

I

M

A

I

A

P

H

M

G

N

L

F

L

L

R

K

-

E

-

G

S

Q

K

T

S

A

Q

N

H

Y

L

V

A

M

F

T

G

G

E

S

F
x
W

S

A

S

S

K

K

F

A

A

L

K

K

V

-

A

-

G

E

A

A

P

K

W

L

L

I

E

G

E

D

P

T

T

H

V

V

V

A

S

A

T

S

D

S

P

E

G

A

S

S

H

N

-

Y

-

M

-

T

-

L

-

P

-

K

-

L

G

Q

E

E

A

I

V

Q

A

L

E

Q

A

D

G

N

V

A

D

A

V

Y

Y

L

A

H

L

L

T

T

H

S

N

N

E

E

T

T

S

I

T

E

G

G

V

A

A

Q

A

F

P

K

I

-

R

-

V

-

A

A

G

F

A

P

D

D

D

T

D

G

A

S

L

V

V

P

L

L

V

I

-

G

D
|
D

A

M

T
x
S

S

S

G

D

A

I

G

L

G

S

L

R

P

P

V

F

D

D

I

L

A

N

E

Q

T

F

D

G

V

L

Y

V

Y

Y

F

A

A

G

P

A

Q
|
Q

K
|
K

S

N

F

L

A

G

A

P

D

S

G

G

G

V

L

T

W

V

I

V

A

I

V

V

M

-

S

-

P

-

R

-

A

-

L

-

A

-

R

R

V

E

E

D

E

L

I

V

A

A

A

E

G

S

G

P

R

K

Y

H

I

L

P

P

E

T

F

M

F

L

S

R

L

Y

T

D

T

T

A

Y

V

V

A

K

N

N

S

-

R

-

K

-

D

N

Q

S

T

L

Y

Y

N

N

T

T

P

P

A

P

V

S

A

F

T

G

L

I

L

Y

L

M

L

V

A

N

E

E

Q

V

L

L

E

K

W

W

M

I

N

E

T

E

R

R

G

G

L

L

E

E

W

G

T

V

V

Q

A

Q

R

A

T

N

A

R

E

K

S

K

S

A

S

S

I

L

L

I

Y

Y

D

D

W

A

A

I

E

D

K

Q

S

S

P

G

V

G

A

F

S

Y

P

R

F

G

V

C

T

V

D

D

P

V

A

D

K

S

R

R

S

S

Q

D

V

M

V

N

G

I

T

T

I

F

D

R

F

L

S

A

D

S

D

E

V

E

D

L

A

E

A

K

A

E

I

F

A

V

K

K

V

A

L

S

R

E

A

Q

N

E

G

G

V

F

V

V

D

G

T

L

E

K

P

G

Y

H

R

R

K

S

L

V

G

G

R

-

N

-

Q

G

L

L

R

R

V

A

A

S

M

I

F

Y

P

N

A

A

V

V

active site: W102 (≠ F104), D172 (= D173), K196 (= K197)
binding pyridoxal-5'-phosphate: A76 (≠ T80), S77 (≠ T81), W102 (≠ F104), D172 (= D173), S174 (≠ T175), Q195 (= Q196), K196 (= K197)

8a5vE Crystal structure of the human phosposerine aminotransferase (psat) (see paper)
23% identity, 93% coverage: 17:362/374 of query aligns to 5:353/366 of 8a5vE

query
sites
8a5vE

R

N

F

F

G

G

S

P

G

G

P

P

S

A

K

K

V

L

R

P

P

H

E

S

Q

V

L

L

E

L

A

E

L

I

A

Q

S

K

S

E

G

L

A

L

R

D

Y

Y

M

K

G

G

T

V

-

G

-

I

-

S

-

V

-

L

-

E

-

M

S

S

H

H

R

R

Q

S

K

S

P

D

V

F

K

A

S

K

L

I

V

I

S

N

R

N

V

T

R

E

S

N

G

L

V

V

S

R

D

E

L

L

F

L

S

A

L

V

P

P

E

D

G

N

Y

Y

E

K

V

V

V

I

L

F

G

L

N

Q

G

G

T

G

T

C

A

G

F

Q

W

F

D

S

I

A

A

V

A

P

H

L

G

N

L

L

L

I

R

G

S

L

K

K

S

A

Q

G

H

R

L

C

A

A

-

D

-

Y

-

V

-

T

-

G

-

A

-
x
W

-

S

-

A

-

K

F

A

G

A

E

E

F

E

S

A

S

K

K

K

F

F

A

G

K

T

V

I

A

N

A

I

G

V

A

H

P

P

W

K

L

L

E

G

E

S

P

Y

T

T

V

K

V

I

S

P

T

-

D

D

P

P

G

-

S

S

H

T

G

W

E

N

A

L

V

N

A

P

E

D

A

A

G

S

V

Y

D

V

V

Y

Y

Y

A

C

L

-

T

-

H

A

N

N

E

E

T

T

S

V

T

H

G

G

V

V

A

E

A

F

P

D

-

F

I

I

R

P

R

D

V

V

A

K

G

G

A

A

D

-

A

-

L

V

V

L

L

V

V

C

D

D

A

M

T

S

S

S

G

N

A

F

G

L

G

S

L

K

P

P

V

V

D

D

I

V

A

S

E

K

T

F

D

G

V

V

Y

I

Y

F

F

A

A

G

P

A

Q

Q

K

K

S

N

F

V

A

G

A

S

D

A

G

G

G

V

L

T

W

V

I

V

A

I

V

V

M

-

S

-

P

-

R

-

A

-

L

-

A

-

R

R

V

D

E

D

E

L

I

L

A

G

A

F

G

A

G

L

R

R

Y

E

I

C

P

P

E

S

F

V

F

L

S

E

L

Y

T

K

T

V

A

Q

V

A

A

G

N

N

S

S

R

-

K

-

D

-

Q

S

T

L

Y

Y

N

N

T

T

P

P

A

P

V

C

A

F

T

S

L

I

L

Y

L

V

L

M

A

G

E

L

Q

V

L

L

E

E

W

W

M

I

N

K

T

N

R

N

G

G

L

A

E

A

W

A

T

M

V

E

A

K

R

L

T

S

A

S

E

I

S

K

S

S

S

Q

I

T

L

I

Y

Y

D

E

W

I

A

I

E

D

K

N

S

S

P

Q

V

G

A

F

S

Y

P

V

F

C

V

P

T

V

D

E

P

P

A

Q

K

N

R

R

S

S

Q

K

V

M

-

N

-

I

-

P

-

F

-

R

V

I

G

G

T

N

I

A

D

K

F

G

S

D

D

D

D

A

V

L

D

E

A

K

A

R

A

F

I

L

A

D

K

K

V

A

L

L

R

E

A

L

N

N

G

-

V

M

V

L

D

S

T

L

E

K

P

G

Y

H

R
|
R

K

S

L

V

G

G

R

-

N

-

Q

G

L

I

R

R

V

A

A

S

M

L

F

Y

P

N

A

A

V

V

E

T

P

I

G

E

D

D

V

V

H

Q

K

K

L

L

binding sulfate ion: W103 (vs. gap), R332 (= R339)

8a5wC Crystal structure of the human phosphoserine aminotransferase (psat) in complex with o-phosphoserine (see paper)
23% identity, 93% coverage: 17:362/374 of query aligns to 4:352/365 of 8a5wC

query
sites
8a5wC

R

N

F

F

G

G

S

P

G

G

P

P

S

A

K

K

V

L

R

P

P

H

E

S

Q

V

L

L

E

L

A

E

L

I

A

Q

S

K

S

E

G

L

A

L

R

D

Y

Y

M

K

G

G

T

V

-

G

-

I

-

S

-

V

-

L

-

E

-

M

S

S

H
|
H

R
|
R

Q

S

K

S

P

D

V

F

K

A

S

K

L

I

V

I

S

N

R

N

V

T

R

E

S

N

G

L

V

V

S

R

D

E

L

L

F

L

S

A

L

V

P

P

E

D

G

N

Y

Y

E

K

V

V

V

I

L

F

G

L

N

Q

G

G

T

G

T

C

A

G

F

Q

W

F

D

S

I

A

A

V

A

P

H

L

G

N

L

L

L

I

R

G

S

L

K

K

S

A

Q

G

H

R

L

C

A

A

-

D

-

Y

-

V

-

T

-

G

-

A

-

W

-

S

-

A

-

K

F

A

G

A

E

E

F

E

S

A

S

K

K

K

F

F

A

G

K

T

V

I

A

N

A

I

G

V

A

H

P

P

W

K

L

L

E

G

E

S

P

Y

T

T

V

K

V

I

S

P

T

-

D

D

P

P

G

-

S

S

H

T

G

W

E

N

A

L

V

N

A

P

E

D

A

A

G

S

V

Y

D

V

V

Y

Y

Y

A

C

L

-

T

-

H

A

N

N

E

E

T
|
T

S

V

T

H

G

G

V

V

A

E

A

F

P

D

-

F

I

I

R

P

R

D

V

V

A

K

G

G

A

A

D

-

A

-

L

V

V

L

L

V

V

C

D

D

A

M

T

S

S

S

G

N

A

F

G

L

G

S

L

K

P

P

V

V

D

D

I

V

A

S

E

K

T

F

D

G

V

V

Y

I

Y

F

F

A

A

G

P

A

Q

Q

K
|
K

S

N

F

V

A

G

A

S

D

A

G

G

G

V

L

T

W

V

I

V

A

I

V

V

M

-

S

-

P

-

R

-

A

-

L

-

A

-

R

R

V

D

E

D

E

L

I

L

A

G

A

F

G

A

G

L

R

R

Y

E

I

C

P

P

E

S

F

V

F

L

S

E

L

Y

T

K

T

V

A

Q

V

A

A

G

N

N

S

S

R

-

K

-

D

-

Q

S

T

L

Y

Y

N
|
N

T
|
T

P

P

A

P

V

C

A

F

T

S

L

I

L

Y

L

V

L

M

A

G

E

L

Q

V

L

L

E

E

W

W

M

I

N

K

T

N

R

N

G

G

L

A

E

A

W

A

T

M

V

E

A

K

R

L

T

S

A

S

E

I

S

K

S

S

S

Q

I

T

L

I

Y

Y

D

E

W

I

A

I

E

D

K

N

S

S

P

Q

V

G

A

F

S

Y

P

V

F

C

V

P

T

V

D

E

P

P

A

Q

K

N

R

R

S

S

Q

K

V

M

-

N

-

I

-

P

-

F

-

R

V

I

G

G

T

N

I

A

D

K

F

G

S

D

D

D

D

A

V

L

D

E

A

K

A

R

A

F

I

L

A

D

K

K

V

A

L

L

R

E

A

L

N

N

G

-

V

M

V

L

D

S

T

L

E

K

P

G

Y
x
H

R
|
R

K

S

L

V

G

G

R

-

N

-

Q

G

L

I

R
|
R

V

A

A

S

M

L

F

Y

P

N

A

A

V

V

E

T

P

I

G

E

D

D

V

V

H

Q

K

K

L

L

binding (2S)-2-[(E)-[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]-3-phosphonooxy-propanoic acid: H39 (= H45), R40 (= R46), N236 (= N248), T237 (= T249)
binding phosphoserine: T151 (= T150), K195 (= K197), H330 (≠ Y338), R331 (= R339), R337 (= R347)

8a5wA Crystal structure of the human phosphoserine aminotransferase (psat) in complex with o-phosphoserine (see paper)
23% identity, 93% coverage: 17:362/374 of query aligns to 4:352/365 of 8a5wA

query
sites
8a5wA

R

N

F

F

G

G

S

P

G

G

P

P

S

A

K

K

V

L

R

P

P

H

E

S

Q

V

L

L

E

L

A

E

L

I

A

Q

S

K

S

E

G

L

A

L

R

D

Y

Y

M

K

G

G

T

V

-

G

-

I

-

S

-

V

-

L

-

E

-

M

S

S

H
|
H

R
|
R

Q

S

K

S

P

D

V

F

K

A

S

K

L

I

V

I

S

N

R

N

V

T

R

E

S

N

G

L

V

V

S

R

D

E

L

L

F

L

S

A

L

V

P

P

E

D

G

N

Y

Y

E

K

V

V

V

I

L

F

G

L

N

Q

G

G

G
|
G

T
x
G

T
x
C

A

G

F

Q

W

F

D

S

I

A

A

V

A

P

H

L

G

N

L

L

L

I

R

G

S

L

K

K

S

A

Q

G

H

R

L

C

A

A

-

D

-

Y

-

V

-

T

-

G

-

A

-
x
W

-

S

-

A

-

K

F

A

G

A

E

E

F

E

S

A

S

K

K

K

F

F

A

G

K

T

V

I

A

N

A

I

G

V

A

H

P

P

W

K

L

L

E

G

E

S

P

Y

T

T

V

K

V

I

S

P

T

-

D

D

P

P

G

-

S

S

H

T

G

W

E

N

A

L

V

N

A

P

E

D

A

A

G

S

V

Y

D

V

V

Y

Y

Y

A

C

L

-

T

-

H

A

N

N

E

E

T
|
T

S

V

T

H

G

G

V

V

A

E

A

F

P

D

-

F

I

I

R

P

R

D

V

V

A

K

G

G

A

A

D

-

A

-

L

V

V

L

L

V

V

C

D
|
D

A

M

T
x
S

S

S

G

N

A

F

G

L

G

S

L

K

P

P

V

V

D

D

I

V

A

S

E

K

T

F

D

G

V

V

Y

I

Y

F

F

A

A

G

P

A

Q
|
Q

K
|
K

S

N

F

V

A

G

A

S

D

A

G

G

G

V

L

T

W

V

I

V

A

I

V

V

M

-

S

-

P

-

R

-

A

-

L

-

A

-

R

R

V

D

E

D

E

L

I

L

A

G

A

F

G

A

G

L

R

R

Y

E

I

C

P

P

E

S

F

V

F

L

S

E

L

Y

T

K

T

V

A

Q

V

A

A

G

N

N

S

S

R

-

K

-

D

-

Q

S

T

L

Y

Y

N

N

T

T

P

P

A

P

V

C

A

F

T

S

L

I

L

Y

L

V

L

M

A

G

E

L

Q

V

L

L

E

E

W

W

M

I

N

K

T

N

R

N

G

G

L

A

E

A

W

A

T

M

V

E

A

K

R

L

T

S

A

S

E

I

S

K

S

S

S

Q

I

T

L

I

Y

Y

D

E

W

I

A

I

E

D

K

N

S

S

P

Q

V

G

A

F

S

Y

P

V

F

C

V

P

T

V

D

E

P

P

A

Q

K

N

R

R

S

S

Q

K

V

M

-

N

-

I

-

P

-

F

-

R

V

I

G

G

T

N

I

A

D

K

F

G

S

D

D

D

D

A

V

L

D

E

A

K

A

R

A

F

I

L

A

D

K

K

V

A

L

L

R

E

A

L

N

N

G

-

V

M

V

L

D

S

T

L

E

K

P

G

Y
x
H

R

R

K

S

L

V

G

G

R

-

N

-

Q

G

L

I

R
|
R

V

A

A

S

M

L

F

Y

P

N

A

A

V

V

E

T

P

I

G

E

D

D

V

V

H

Q

K

K

L

L

binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G73 (= G79), G74 (≠ T80), C75 (≠ T81), W102 (vs. gap), T151 (= T150), D171 (= D173), S173 (≠ T175), Q194 (= Q196), K195 (= K197)
binding phosphoserine: H39 (= H45), R40 (= R46), H330 (≠ Y338), R337 (= R347)

8a5vA Crystal structure of the human phosposerine aminotransferase (psat) (see paper)
23% identity, 93% coverage: 17:362/374 of query aligns to 4:352/365 of 8a5vA

query
sites
8a5vA

R

N

F

F

G

G

S

P

G

G

P

P

S

A

K

K

V

L

R

P

P

H

E

S

Q

V

L

L

E

L

A

E

L

I

A

Q

S

K

S

E

G

L

A

L

R

D

Y

Y

M

K

G

G

T

V

-

G

-

I

-

S

-

V

-

L

-

E

-

M

S

S

H

H

R

R

Q

S

K

S

P

D

V

F

K

A

S

K

L

I

V

I

S

N

R

N

V

T

R

E

S

N

G

L

V

V

S

R

D

E

L

L

F

L

S

A

L

V

P

P

E

D

G

N

Y

Y

E

K

V

V

V

I

L

F

G

L

N

Q

G

G

T

G

T

C

A

G

F

Q

W

F

D

S

I

A

A

V

A

P

H

L

G

N

L

L

L

I

R

G

S

L

K

K

S

A

Q

G

H

R

L

C

A

A

-

D

-

Y

-

V

-

T

-

G

-

A

-

W

-

S

-

A

-

K

F

A

G

A

E

E

F

E

S

A

S

K

K

K

F

F

A

G

K

T

V

I

A

N

A

I

G

V

A

H

P

P

W

K

L

L

E

G

E

S

P

Y

T

T

V

K

V

I

S

P

T

-

D

D

P

P

G

-

S

S

H

T

G

W

E

N

A

L

V

N

A

P

E

D

A

A

G

S

V

Y

D

V

V

Y

Y

Y

A

C

L

-

T

-

H

A

N

N

E

E

T

T

S

V

T

H

G

G

V

V

A

E

A

F

P

D

-

F

I

I

R

P

R

D

V

V

A

K

G

G

A

A

D

-

A

-

L

V

V

L

L

V

V

C

D

D

A

M

T

S

S

S

G

N

A

F

G

L

G

S

L

K

P

P

V

V

D

D

I

V

A

S

E

K

T

F

D

G

V

V

Y

I

Y

F

F

A

A

G

P

A

Q

Q

K

K

S

N

F

V

A

G

A

S

D

A

G

G

G

V

L

T

W

V

I

V

A

I

V

V

M

-

S

-

P

-

R

-

A

-

L

-

A

-

R

R

V

D

E

D

E

L

I

L

A

G

A

F

G

A

G

L

R

R

Y

E

I

C

P

P

E

S

F

V

F

L

S

E

L

Y

T

K

T

V

A

Q

V

A

A

G

N

N

S

S

R

-

K

-

D

-

Q

S

T

L

Y

Y

N
|
N

T
|
T

P

P

A

P

V

C

A

F

T

S

L

I

L

Y

L

V

L

M

A

G

E

L

Q

V

L

L

E

E

W

W

M

I

N

K

T

N

R

N

G

G

L

A

E

A

W

A

T

M

V

E

A

K

R

L

T

S

A

S

E

I

S

K

S

S

S

Q

I

T

L

I

Y

Y

D

E

W

I

A

I

E

D

K

N

S

S

P

Q

V

G

A

F

S

Y

P

V

F

C

V

P

T

V

D

E

P

P

A

Q

K

N

R

R

S

S

Q

K

V

M

-

N

-

I

-

P

-

F

-

R

V

I

G

G

T

N

I

A

D

K

F

G

S

D

D

D

D

A

V

L

D

E

A

K

A

R

A

F

I

L

A

D

K

K

V

A

L

L

R

E

A

L

N

N

G

-

V

M

V

L

D

S

T

L

E

K

P

G

Y

H

R

R

K

S

L

V

G

G

R

-

N

-

Q

G

L

I

R

R

V

A

A

S

M

L

F

Y

P

N

A

A

V

V

E

T

P

I

G

E

D

D

V

V

H

Q

K

K

L

L

binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: N236 (= N248), T237 (= T249)

2dr1A Crystal structure of the ph1308 protein from pyrococcus horikoshii ot3
21% identity, 97% coverage: 13:374/374 of query aligns to 15:376/381 of 2dr1A

query
sites
2dr1A

P

P

S

K

D

Y

G

K

R

L

F

F

G

T

S

A

G

G

P

P

S

V

K

A

V

C

R

F

P

P

E

E

Q

V

L

L

E

E

A

I

L

M

A

-

S

-

G

-

A

-

R

K

Y

V

M

Q

G

M

T

F

S

S

H

H

R

R

Q

S

K

K

P

E

V

Y

K

R

S

K

L

V

V

H

S

M

R

D

V

T

R

V

S

E

G

R

V

L

S

R

D

E

L

F

F

L

S

E

L

V

P

E

E

K

G

G

Y

E

E

V

V

L

L

V

G

P

N

S

G
x
S

G
|
G

T
|
T

T

G

A

I

F

M

W

E

D

A

I

S

A

I

A

R

H

N

G

G

L

V

L

S

R

K

S

G

-

G

K

K

S

V

Q

L

H

V

L

T

A

I

F

I

G

G

E

A

F
|
F

S

G

S

K

K

R

F

Y

A

K

K

E

V

V

A

V

A

E

G

S

A

N

P

G

W

-

L

-

E

R

E

K

P

A

T

V

V

V

V

L

S

E

T

Y

D

E

P

P

G

G

S

K

-

A

-

V

H

K

G

P

E

E

A

D

V

L

A

D

E

D

A

A

-

L

-

R

-

K

-

N

-

P

G

D

V

V

D

E

V

A

Y

V

A

T

L

I

T

T

H

Y

N

N

E

E

T
|
T

S

S

T

T

G

G

V

V

A

L

A

N

P

P

I

L

R

P

R

E

V

L

A

A

-

K

-

V

G

A

A

K

D

E

D

H

D

D

A

K

L

L

V

V

L

F

V

V

D
|
D

A

A

T
x
V

S

S

G

A

A

M

G

G

-

A

-

D

L

I

P

K

V

F

D

D

I

K

A

W

E

G

T

L

D

D

V

V

Y

V

Y

F

F

S

A
x
S

P

S

Q

Q

K
|
K

S

A

F

F

A

G

A

V

D

P

G

P

G

G

L

L

W

A

I

I

A

G

V

A

M

F

S

S

P

E

R

R

A

F

L

L

A

E

R

I

V

A

E

E

E

K

I

M

A

P

A

E

G

R

G

G

R

W

Y

Y

I

-

P

-

E

-

F

-

F

F

S

D

L

I

T

P

T

L

A

Y

V

V

A

K

N

Y

S

L

R

K

-

E

K

K

D

E

Q

S

T

T

Y

P

N

S

T

T

P

P

A

P

V

M

A

P

T

Q

L

V

L

F

L

G

L

I

A

N

E

V

Q

A

L

L

E

R

W

I

M

I

N

E

T

K

R

M

G

G

L

K

E

E

W

K

T

W

V

L

A

E

R

M

T

Y

A

E

E

K

S

R

S

A

S

K

I

M

L

V

Y

R

D

E

W

G

A

V

E

R

K

E

S

-

P

-

V

I

A

G

S

L

P

D

F

I

V

L

T

A

D

E

P

P

A

G

K

H

R

E

S

S

Q

P

V

T

V

I

G

T

T

A

I

V

D

L

F

T

S

P

D

P

D

G

V

I

D

K

A

G

A

D

A

E

I

V

A

Y

K

E

V

A

L

M

R

R

A

K

N

R

G

G

V

F

V

E

D

L

T

A

E

K

P

G

Y

Y

R

G

K

S

L

V

G

K

R

E

N

K

Q

T

L

F

R

R

V

I

A

G

M

H

F

M

P

G

A

Y

V

M

E

K

P

F

G

E

D

D

V

I

H

Q

K

E

L

M

T

L

E

D

C

N

V

L

D

R

F

E

V

V

L

I

S

N

R

E

L

L

S

K

binding pyridoxal-5'-phosphate: S75 (≠ G78), G76 (= G79), T77 (= T80), F102 (= F104), T153 (= T150), D178 (= D173), V180 (≠ T175), S201 (≠ A194), K204 (= K197)

1iugA The crystal structure of aspartate aminotransferase which belongs to subgroup iv from thermus thermophilus (see paper)
26% identity, 84% coverage: 21:333/374 of query aligns to 7:307/348 of 1iugA

query
sites
1iugA

G

G

P

P

S

V

K

R

V

L

R

H

P

P

E

K

Q

A

L

L

E

E

A

A

L

L

A

A

S

R

S

P

G

Q

A

-

R

-

Y

-

M

-

G

-

T

L

S

H

H

H

R

R

Q

T

K

E

P

A

V

A

K

R

S

E

L

V

V

F

S

L

R

K

V

A

R

R

S

G

G

L

V

L

S

R

D

E

L

A

F

F

S

R

L

T

P

-

E

E

G

G

Y

E

E

V

V

L

V

I

L

L

G

T

N

G

G

S

G

G

T

T

T

L

A

A

F

M

W

E

D

A

I

L

A

V

A

K

H

N

G

-

L

L

L

F

R

A

S

P

K

G

S

E

Q

R

H

V

L

L

A

V

-

P

-

V

F

Y

G

G

E
x
K

F

F

S

S

S

E

K

R

F

F

A

Y

K

E

V

I

A

A

A

L

G

E

A

A

P

G

W

L

L

V

E

V

E

E

P

R

T

L

V

D

V

Y

S

P

T

Y

D

G

P

D

G

T

S

P

H

R

G

P

E

E

A

D

V

V

A

A

E

K

A

E

G

G

V

Y

D

A

V

G

Y

L

A

L

L

L

T

V

H

H

N

S

E

E

T

T

S

S

T

T

G

G

V

A

A

L

A

A

P

D

I

L

R

P

R

A

V

L

A

A

G

R

A

A

-

F

-

K

-

E

-

K

D

N

D

P

D

E

A

G

L

L

V

V

L

G

V

A

D

D

A

M

T

V

S

T

G

S

-

L

A

V

G

G

G

E

L

V

P

A

V

L

D

E

I

A

A

M

E

G

T

V

D

D

V

A

Y

A

F

S

A

G

P

S

Q

Q

K
|
K

S

G

F

L

A

M

A

C

D

P

G

P

G

G

L

L

W

G

I

F

A

V

V

A

M

L

S

S

P

P

R

R

A

A

L

L

A

E

R

R

V

L

E

K

E

-

I

-

A

-

A

P

G

R

G

G

R

Y

Y

Y

I

L

P

-

E

-

F

-

S

D

L

L

T

A

T

R

A

E

V

L

A

K

N

A

S

Q

R

K

K

E

D

G

Q

E

T

S

Y

A

N

W

T

T

P

P

A

A

V

I

A

N

T

L

L

V

L

L

L

A

L

V

A

A

E

A

Q

V

L

L

E

E

W

E

M

V

N

L

T

P

R

R

G

-

L

L

E

E

W

E

T

H

V

L

A

A

R

L

T

K

A

A

E

W

S

Q

S

N

S

A

I

L

L

L

Y

Y

D

G

W

V

A

G

E

E

K

E

S

G

-

G

-

L

-

R

P

P

V

V

A

-

S

-

P

-

F

-

V

-

T

-

D

-

P

P

A

K

K

R

R

F

S

S

Q

P

V

A

V

V

G

A

T

A

I

F

D

Y

F

L

S

P

D

E

D

G

V

V

D

P

A

Y

A

A

A

R

I

V

A

K

K

E

V

A

L

F

R

A

A

Q

N

R

G

G

V

A

V

V

binding phosphate ion: K84 (≠ E103), K184 (= K197)

8a5wE Crystal structure of the human phosphoserine aminotransferase (psat) in complex with o-phosphoserine (see paper)
23% identity, 93% coverage: 17:362/374 of query aligns to 5:352/365 of 8a5wE

query
sites
8a5wE

R

N

F

F

G

G

S

P

G

G

P

P

S

A

K

K

V

L

R

P

P

H

E

S

Q

V

L

L

E

L

A

E

L

I

A

Q

S

K

S

E

G

L

A

L

R

D

Y

Y

M

K

G

G

T

V

-

G

-

I

-

S

-

V

-

L

-

E

-

M

S

S

H
|
H

R
|
R

Q

S

K

S

P

D

V

F

K

A

S

K

L

I

V

I

S

N

R

N

V

T

R

E

S

N

G

L

V

V

S

R

D

E

L

L

F

L

S

A

L

V

P

P

E

D

G

N

Y

Y

E

K

V

V

V

I

L

F

G

L

N

Q

G

G

G
|
G

T
x
G

T
x
C

A

G

F

Q

W

F

D

S

I

A

A

V

A

P

H

L

G

N

L

L

L

I

R

G

S

L

K

K

S

A

Q

G

H

R

L

C

A

A

-

D

-

Y

-

V

-

T

-

G

-

A

-
x
W

-

S

-

A

-

K

F

A

G

A

E

E

F

E

S

A

S

K

K

K

F

F

A

G

K

T

V

I

A

N

A

I

G

V

A

H

P

P

W

K

L

L

E

G

E

S

P

Y

T

T

V

K

V

I

S

P

T

-

D

D

P

P

G

-

S

S

H

T

G

W

E

N

A

L

V

N

A

P

E

D

A

A

G

S

V

Y

D

V

V

Y

Y

Y

A

C

L

-

T

-

H

A

N

N

E

E

T
|
T

S

V

T

H

G

G

V

V

A

E

A

F

P

D

-

F

I

I

R

P

R

D

V

V

A

K

G

G

A

A

D

-

A

-

L

V

V

L

L

V

V

C

D

D

A

M

T
x
S

S

S

G

N

A

F

G

L

G

S

L

K

P

P

V

V

D

D

I

V

A

S

E

K

T

F

D

G

V

V

Y

I

Y

F

F

A

A

G

P
x
A

Q
|
Q

K
x
N

S

V

F

G

A

S

A

A

D

-

G

-

G

G

L

V

W

T

I

V

A

V

V

I

M

V

S

-

P

-

R

-

A

-

L

-

A

-

R

R

V

D

E

D

E

L

I

L

A

G

A

F

G

A

G

L

R

R

Y

E

I

C

P

P

E

S

F

V

F

L

S

E

L

Y

T

K

T

V

A

Q

V

A

A

G

N

N

S

S

R

-

K

-

D

-

Q

S

T

L

Y

Y

N
|
N

T
|
T

P

P

A

P

V

C

A

F

T

S

L

I

L

Y

L

V

L

M

A

G

E

L

Q

V

L

L

E

E

W

W

M

I

N

K

T

N

R

N

G

G

L

A

E

A

W

A

T

M

V

E