SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_026142645.1 NCBI__GCF_000816635.1:WP_026142645.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P26276 Phosphomannomutase/phosphoglucomutase; PMM / PGM; EC 5.4.2.2; EC 5.4.2.8 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 10 papers)
28% identity, 84% coverage: 57:485/508 of query aligns to 51:444/463 of P26276

query
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P26276

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S108 (= S116) binding via phosphate group; modified: Phosphoserine; mutation S->A,V: About 5% activity, still subject to substrate inhibition and requires G1,6P as an activator; phosphorylation occurs at a different site.; mutation to C: KM for G1P unchanged, kcat decreases 24-fold; G1,6P stimulates reaction by 2-3 orders of magnitude. No stable protein phosphorylation detected, altered ligation of metal residue.
N110 (≠ L118) mutation to A: KM halves, decreases processivity as dissociation of G1,6P intermediate increases 30-fold.
D242 (= D264) binding Mg(2+)
D244 (= D266) binding Mg(2+)
D246 (= D268) binding Mg(2+)
R247 (= R269) mutation to A: Small reduction in KM, small increase in dissociation of G1,6P intermediate.
R262 (≠ L284) mutation to A: Increases KM 2-fold, decreases kcat 9-fold for G1P. Alters flexibility of the hinge region.
K285 (≠ V307) binding alpha-D-glucose 1-phosphate
H308 (≠ Y330) binding alpha-D-glucose 1-phosphate; binding alpha-D-mannose 1-phosphate
E325 (= E352) mutation to A: Reduces KM and Vmax approximately 2-fold.
EMSGH 325:329 (≠ ETSGH 352:356) binding alpha-D-glucose 1-phosphate; binding alpha-D-mannose 1-phosphate
H329 (= H356) mutation to A: No phosphoglucomutase activity using G1P as substrate, protein is less easily phosphorylated, no significant change in structure.
P368 (≠ I394) mutation to G: Increases KM 2-fold, decreases kcat 6-fold for G1P. Alters flexibility of the hinge region, structure is less compact.
R421 (= R462) mutation to C: Loss of phosphomannomutase activity, very low phosphoglucomutase activity.
RASNT 421:425 (≠ RLSLH 462:466) binding alpha-D-glucose 1-phosphate; binding alpha-D-mannose 1-phosphate

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
15 R→A: KM halves, decreases processivity as dissociation of G1,6P intermediate increases 25-fold.
17 binding alpha-D-glucose 1-phosphate; binding alpha-D-mannose 1-phosphate
20 R→A: No phosphoglucomutase activity.

Q02E40 Phosphomannomutase/phosphoglucomutase; PMM / PGM; EC 5.4.2.2; EC 5.4.2.8 from Pseudomonas aeruginosa (strain UCBPP-PA14) (see paper)
28% identity, 84% coverage: 57:485/508 of query aligns to 51:444/463 of Q02E40

query
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Q02E40

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S108 (= S116) active site, Non-phosphorylated intermediate; modified: Phosphoserine

2h5aX Complex of the enzyme pmm/pgm with xylose 1-phosphate (see paper)
28% identity, 84% coverage: 57:485/508 of query aligns to 43:436/455 of 2h5aX

query
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2h5aX

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A

S

R

Q

L

D

K

Q

N

R

D

D

S

S

N

E

G

H

K

V

L

F

S

S

D

A

L

F

I

P

S

S

D

D

L

I

D

S

S

T

P

P

C

-

E

-

S

-

K

-

E

-

Y

-

R

E

M

I

K

N

I

I

K

T

F

V

N

T

D

E

F

D

K

S

E

K

Y

F

G

A

S

-

N

-

I

I

L

I

D

E

N

A

L

L

K

Q

D

R

Y

D

V

A

K

Q

S

-

I

-

N

-

G

-

W

W

G

G

-

E

I

G

E

N

P

I

E

T

N

T

Y

L

E

D

G

G

I

V

K

R

V

V

N

D

C

Y

N

P

K

K

E

-

N

-

G

-

D

-

G

G

W

W

F

G

L

L

L

V

R
|
R

L

A

S
|
S

L
x
N

H
x
T

E

T

A

P

V

V

M

L

P

V

L

L

N

R

I

F

E

E

S

A

D

D

S

T

Q

E

G

E

V

L

E

E

F

R

I

I

active site: H101 (= H117), D234 (= D264), D236 (= D266), D238 (= D268), R239 (= R269), D332 (= D366)
binding 1-O-phosphono-alpha-D-xylopyranose: T298 (≠ R328), G299 (= G329), H300 (≠ Y330), E317 (= E352), S319 (= S354), H321 (= H356), R413 (= R462), S415 (= S464), N416 (≠ L465), T417 (≠ H466)
binding zinc ion: S100 (= S116), D234 (= D264), D236 (= D266), D238 (= D268)

Sites not aligning to the query:

binding 1-O-phosphono-alpha-D-xylopyranose: 9

2h4lX Complex of pmm/pgm with ribose 1-phosphate (see paper)
28% identity, 84% coverage: 57:485/508 of query aligns to 43:436/455 of 2h4lX

query
sites
2h4lX

I

V

S

A

I

V

G

G

M

R

D

D

S

G

R

R

I

L

T

S

S

G

E

P

S

E

I

L

K

V

E

K

I

Q

L

L

I

I

S

Q

E

G

I

L

K

V

K

D

L

C

G

G

C

C

T

Q

V

V

M

S

D

D

C

V

G

G

L

M

C

V

S

P

T

T

P

P

A

V

M

L

F

Y

M

Y

S

A

T

A

I

N

F

V

E

L

N

E

Y

G

K

K

S

S

D

-

G

-

A

G

I

V

E

M

I

L

T

T

A

G

S
|
S

H
|
H

L

N

P

P

Y

P

Y

D

Y

Y

N

N

G

G

F

F

K

K

F

I

F

V

T

V

N

A

K

G

G

E

G

T

F

L

D

A

K

N

E

E

D

Q

I

I

N

Q

E

A

L

L

L

R

D

E

I

-

A

-

Q

R

N

I

E

E

K

K

V

N

C

D

T

L

V

A

Q

S

K

G

L

V

G

G

A

S

I

V

I

E

K

Q

C

V

D

D

L

I

M

L

K

P

D

R

Y

Y

S

-

N

-

F

-

L

-

I

-

N

-

K

-

I

-

I

F

D

K

G

Q

I

I

D

R

D

D

K

D

E

I

H

A

R

M

L

A

K

K

P

P

L

M

E

-

N

-

F

-

K

K

I

V

V

V

D

D

A

C

G

G

N

N

G

G

A

V

G

A

G

G

F

V

F

I

V

A

E

P

K

Q

V

L

L

I

N

E

Q

A

L

L

G

G

A

C

D

S

T

V

N

I

G

-

S

P

Q

L

F

Y

I

C

E

E

P

V

D

D

G

G

T

N

F

F

P

P

N

N

H

H

I

H

P

P

N

D

P

P

E

G

K

K

K

P

E

E

A

N

M

L

D

K

S

D

L

L

K

I

K

A

A

K

V

V

L

K

E

A

N

E

K

N

A

A

D

D

I

L

G

G

I

L

I

A

F

F

D
|
D

T

G

D
|
D

V

G

D
|
D

R
|
R

A

V

A

G

I

V

V

V

D

T

S

N

Y

T

G

G

R

T

E

I

I

I

N

Y

R

P

N

D

L

R

L

L

I

L

A

M

L

L

T

F

S

A

A

K

I

D

V

V

L

V

E

S

E

R

H

N

P

P

G

G

S

A

I

D

I

I

V

I

T

F

D

D

S

V

V

K

T

C

S

T

N

R

G

R

L

L

K

I

K

A

F

L

I

I

E

S

K

G

R

Y

G

G

I

R

H

P

H

V

R

M

F

W

K

K

R
x
T

G

G

Y

H

R

-

N

-

V

-

I

-

N

-

E

S

A

L

I

I

K

K

F

K

N

K

N

M

E

K

S

E

R

T

E

G

C

A

H

L

F

L

A

A

I

G

E
|
E

T

M

S

S

G

G

H

H

A

V

A

F

L

F

K

K

E

E

N

R

Y

W

F

F

-

G

L

F

D
|
D

D

D

G

G

A

I

Y

Y

L

S

V

A

C

A

K

R

I

L

L

L

I

E

K

I

M

L

A

S

R

Q

L

D

K

Q

N

R

D

D

S

S

N

E

G

H

K

V

L

F

S

S

D

A

L

F

I

P

S

S

D

D

L

I

D

S

S

T

P

P

C

-

E

-

S

-

K

-

E

-

Y

-

R

E

M

I

K

N

I

I

K

T

F

V

N

T

D

E

F

D

K

S

E

K

Y

F

G

A

S

-

N

-

I

I

L

I

D

E

N

A

L

L

K

Q

D

R

Y

D

V

A

K

Q

S

-

I

-

N

-

G

-

W

W

G

G

-

E

I

G

E

N

P

I

E

T

N

T

Y

L

E

D

G

G

I

V

K

R

V

V

N

D

C

Y

N

P

K

K

E

-

N

-

G

-

D

-

G

G

W

W

F

G

L

L

L

V

R
|
R

L

A

S
|
S

L
x
N

H
x
T

E

T

A

P

V

V

M

L

P

V

L

L

N

R

I

F

E

E

S

A

D

D

S

T

Q

E

G

E

V

L

E

E

F

R

I

I

active site: H101 (= H117), D234 (= D264), D236 (= D266), D238 (= D268), R239 (= R269), D332 (= D366)
binding 1-O-phosphono-alpha-D-ribofuranose: S100 (= S116), T298 (≠ R328), E317 (= E352), R413 (= R462), S415 (= S464), N416 (≠ L465), T417 (≠ H466)
binding zinc ion: S100 (= S116), D234 (= D264), D236 (= D266), D238 (= D268)

Sites not aligning to the query:

binding 1-O-phosphono-alpha-D-ribofuranose: 9, 12

2fkfA Phosphomannomutase/phosphoglucomutase from pseudomonas aeruginosa with alpha-d-glucose 1,6-bisphosphate bound (see paper)
28% identity, 84% coverage: 57:485/508 of query aligns to 43:436/455 of 2fkfA

query
sites
2fkfA

I

V

S

A

I

V

G

G

M

R

D

D

S

G

R

R

I

L

T

S

S

G

E

P

S

E

I

L

K

V

E

K

I

Q

L

L

I

I

S

Q

E

G

I

L

K

V

K

D

L

C

G

G

C

C

T

Q

V

V

M

S

D

D

C

V

G

G

L

M

C

V

S

P

T

T

P

P

A

V

M

L

F

Y

M

Y

S

A

T

A

I

N

F

V

E

L

N

E

Y

G

K

K

S

S

D

-

G

-

A

G

I

V

E

M

I

L

T

T

A

G

S
|
S

H
|
H

L

N

P

P

Y

P

Y

D

Y

Y

N

N

G

G

F

F

K
|
K

F

I

F

V

T

V

N

A

K

G

G

E

G

T

F

L

D

A

K

N

E

E

D

Q

I

I

N

Q

E

A

L

L

L

R

D

E

I

-

A

-

Q

R

N

I

E

E

K

K

V

N

C

D

T

L

V

A

Q

S

K

G

L

V

G

G

A

S

I

V

I

E

K

Q

C

V

D

D

L

I

M

L

K

P

D

R

Y

Y

S

-

N

-

F

-

L

-

I

-

N

-

K

-

I

-

I

F

D

K

G

Q

I

I

D

R

D

D

K

D

E

I

H

A

R

M

L

A

K

K

P

P

L

M

E

-

N

-

F

-

K

K

I

V

V

V

D

D

A

C

G

G

N

N

G

G

A

V

G

A

G

G

F

V

F

I

V

A

E

P

K

Q

V

L

L

I

N

E

Q

A

L

L

G

G

A

C

D

S

T

V

N

I

G

-

S

P

Q

L

F

Y

I

C

E

E

P

V

D

D

G

G

T

N

F

F

P

P

N

N

H

H

I

H

P

P

N

D

P

P

E

G

K

K

K

P

E

E

A

N

M

L

D

K

S

D

L

L

K

I

K

A

A

K

V

V

L

K

E

A

N

E

K

N

A

A

D

D

I

L

G

G

I

L

I

A

F

F

D
|
D

T

G

D
|
D

V

G

D
|
D

R
|
R

A

V

A

G

I

V

V

V

D

T

S

N

Y

T

G

G

R

T

E

I

I

I

N

Y

R

P

N

D

L

R

L

L

I

L

A

M

L

L

T

F

S

A

A

K

I

D

V

V

L

V

E

S

E

R

H

N

P

P

G

G

S

A

I

D

I

I

V

I

T

F

D

D

S

V

V

K

T

C

S

T

N

R

G

R

L

L

K

I

K

A

F

L

I

I

E

S

K

G

R

Y

G

G

I

R

H

P

H

V

R

M

F

W

K

K

R

T

G

G

Y

H

R

-

N

-

V

-

I

-

N

-

E

S

A

L

I

I

K

K

F

K

N

K

N

M

E

K

S

E

R

T

E

G

C

A

H

L

F

L

A

A

I

G

E

E

T

M

S
|
S

G

G

H
|
H

A

V

A

F

L

F

K

K

E

E

N

R

Y

W

F

F

-

G

L

F

D
|
D

D

D

G

G

A

I

Y

Y

L

S

V

A

C

A

K

R

I

L

L

L

I

E

K

I

M

L

A

S

R

Q

L

D

K

Q

N

R

D

D

S

S

N

E

G

H

K

V

L

F

S

S

D

A

L

F

I

P

S

S

D

D

L

I

D

S

S

T

P

P

C

-

E

-

S

-

K

-

E

-

Y

-

R

E

M

I

K

N

I

I

K

T

F

V

N

T

D

E

F

D

K

S

E

K

Y

F

G

A

S

-

N

-

I

I

L

I

D

E

N

A

L

L

K

Q

D

R

Y

D

V

A

K

Q

S

-

I

-

N

-

G

-

W

W

G

G

-

E

I

G

E

N

P

I

E

T

N

T

Y

L

E

D

G

G

I

V

K

R

V

V

N

D

C

Y

N

P

K

K

E

-

N

-

G

-

D

-

G

G

W

W

F

G

L

L

L

V

R
|
R

L

A

S
|
S

L
x
N

H
x
T

E

T

A

P

V

V

M

L

P

V

L

L

N

R

I

F

E

E

S

A

D

D

S

T

Q

E

G

E

V

L

E

E

F

R

I

I

active site: S100 (= S116), H101 (= H117), K110 (= K126), D234 (= D264), D236 (= D266), D238 (= D268), R239 (= R269), H321 (= H356), D332 (= D366)
binding 1,6-di-O-phosphono-alpha-D-glucopyranose: H101 (= H117), S319 (= S354), R413 (= R462), S415 (= S464), N416 (≠ L465), T417 (≠ H466)
binding zinc ion: S100 (= S116), D234 (= D264), D236 (= D266), D238 (= D268)

Sites not aligning to the query:

active site: 12
binding 1,6-di-O-phosphono-alpha-D-glucopyranose: 7

1pcmX Enzyme-ligand complex of p. Aeruginosa pmm/pgm (see paper)
28% identity, 84% coverage: 57:485/508 of query aligns to 43:436/455 of 1pcmX

query
sites
1pcmX

I

V

S

A

I

V

G

G

M

R

D

D

S

G

R

R

I

L

T

S

S

G

E

P

S

E

I

L

K

V

E

K

I

Q

L

L

I

I

S

Q

E

G

I

L

K

V

K

D

L

C

G

G

C

C

T

Q

V

V

M

S

D

D

C

V

G

G

L

M

C

V

S

P

T

T

P

P

A

V

M

L

F

Y

M

Y

S

A

T

A

I

N

F

V

E

L

N

E

Y

G

K

K

S

S

D

-

G

-

A

G

I

V

E

M

I

L

T

T

A

G

S
|
S

H
|
H

L

N

P

P

Y

P

Y

D

Y

Y

N

N

G

G

F

F

K
|
K

F

I

F

V

T

V

N

A

K

G

G

E

G

T

F

L

D

A

K

N

E

E

D

Q

I

I

N

Q

E

A

L

L

L

R

D

E

I

-

A

-

Q

R

N

I

E

E

K

K

V

N

C

D

T

L

V

A

Q

S

K

G

L

V

G

G

A

S

I

V

I

E

K

Q

C

V

D

D

L

I

M

L

K

P

D

R

Y

Y

S

-

N

-

F

-

L

-

I

-

N

-

K

-

I

-

I

F

D

K

G

Q

I

I

D

R

D

D

K

D

E

I

H

A

R

M

L

A

K

K

P

P

L

M

E

-

N

-

F

-

K

K

I

V

V

V

D

D

A

C

G

G

N

N

G

G

A

V

G

A

G

G

F

V

F

I

V

A

E

P

K

Q

V

L

L

I

N

E

Q

A

L

L

G

G

A

C

D

S

T

V

N

I

G

-

S

P

Q

L

F

Y

I

C

E

E

P

V

D

D

G

G

T

N

F

F

P

P

N

N

H

H

I

H

P

P

N

D

P

P

E

G

K

K

K

P

E

E

A

N

M

L

D

K

S

D

L

L

K

I

K

A

A

K

V

V

L

K

E

A

N

E

K

N

A

A

D

D

I

L

G

G

I

L

I

A

F

F

D
|
D

T

G

D
|
D

V

G

D
|
D

R
|
R

A

V

A

G

I

V

V

V

D

T

S

N

Y

T

G

G

R

T

E

I

I

I

N

Y

R

P

N

D

L

R

L

L

I

L

A

M

L

L

T

F

S

A

A

K

I

D

V

V

L

V

E

S

E

R

H

N

P

P

G

G

S

A

I

D

I

I

V

I

T

F

D

D

S

V

V

K

T

C

S

T

N

R

G

R

L

L

K

I

K

A

F

L

I

I

E

S

K

G

R

Y

G

G

I

R

H

P

H

V

R

M

F

W

K

K

R
x
T

G
|
G

Y
x
H

R

-

N

-

V

-

I

-

N

-

E

S

A

L

I

I

K

K

F

K

N

K

N

M

E

K

S

E

R

T

E

G

C

A

H

L

F

L

A

A

I

G

E
|
E

T

M

S
|
S

G

G

H
|
H

A

V

A

F

L

F

K

K

E

E

N

R

Y

W

F

F

-

G

L

F

D
|
D

D

D

G

G

A

I

Y

Y

L

S

V

A

C

A

K

R

I

L

L

L

I

E

K

I

M

L

A

S

R

Q

L

D

K

Q

N

R

D

D

S

S

N

E

G

H

K

V

L

F

S

S

D

A

L

F

I

P

S

S

D

D

L

I

D

S

S

T

P

P

C

-

E

-

S

-

K

-

E

-

Y

-

R

E

M

I

K

N

I

I

K

T

F

V

N

T

D

E

F

D

K

S

E

K

Y

F

G

A

S

-

N

-

I

I

L

I

D

E

N

A

L

L

K

Q

D

R

Y

D

V

A

K

Q

S

-

I

-

N

-

G

-

W

W

G

G

-

E

I

G

E

N

P

I

E

T

N

T

Y

L

E

D

G

G

I

V

K

R

V

V

N

D

C

Y

N

P

K

K

E

-

N

-

G

-

D

-

G

G

W

W

F

G

L

L

L

V

R
|
R

L

A

S
|
S

L

N

H

T

E

T

A

P

V

V

M

L

P

V

L

L

N

R

I

F

E

E

S

A

D

D

S

T

Q

E

G

E

V

L

E

E

F

R

I

I

active site: S100 (= S116), H101 (= H117), K110 (= K126), D234 (= D264), D236 (= D266), D238 (= D268), R239 (= R269), H321 (= H356), D332 (= D366)
binding 6-O-phosphono-alpha-D-mannopyranose: S100 (= S116), T298 (≠ R328), G299 (= G329), H300 (≠ Y330), E317 (= E352), S319 (= S354), H321 (= H356), R413 (= R462), S415 (= S464)
binding zinc ion: S100 (= S116), D234 (= D264), D236 (= D266), D238 (= D268)

Sites not aligning to the query:

active site: 12
binding 6-O-phosphono-alpha-D-mannopyranose: 9

1p5gX Enzyme-ligand complex of p. Aeruginosa pmm/pgm (see paper)
28% identity, 84% coverage: 57:485/508 of query aligns to 43:436/455 of 1p5gX

query
sites
1p5gX

I

V

S

A

I

V

G

G

M

R

D

D

S

G

R

R

I

L

T

S

S

G

E

P

S

E

I

L

K

V

E

K

I

Q

L

L

I

I

S

Q

E

G

I

L

K

V

K

D

L

C

G

G

C

C

T

Q

V

V

M

S

D

D

C

V

G

G

L

M

C

V

S

P

T

T

P

P

A

V

M

L

F

Y

M

Y

S

A

T

A

I

N

F

V

E

L

N

E

Y

G

K

K

S

S

D

-

G

-

A

G

I

V

E

M

I

L

T

T

A

G

S
|
S

H
|
H

L

N

P

P

Y

P

Y

D

Y

Y

N

N

G

G

F

F

K
|
K

F

I

F

V

T

V

N

A

K

G

G

E

G

T

F

L

D

A

K

N

E

E

D

Q

I

I

N

Q

E

A

L

L

L

R

D

E

I

-

A

-

Q

R

N

I

E

E

K

K

V

N

C

D

T

L

V

A

Q

S

K

G

L

V

G

G

A

S

I

V

I

E

K

Q

C

V

D

D

L

I

M

L

K

P

D

R

Y

Y

S

-

N

-

F

-

L

-

I

-

N

-

K

-

I

-

I

F

D

K

G

Q

I

I

D

R

D

D

K

D

E

I

H

A

R

M

L

A

K

K

P

P

L

M

E

-

N

-

F

-

K

K

I

V

V

V

D

D

A

C

G

G

N

N

G

G

A

V

G

A

G

G

F

V

F

I

V

A

E

P

K

Q

V

L

L

I

N

E

Q

A

L

L

G

G

A

C

D

S

T

V

N

I

G

-

S

P

Q

L

F

Y

I

C

E

E

P

V

D

D

G

G

T

N

F

F

P

P

N

N

H

H

I

H

P

P

N

D

P

P

E

G

K

K

K

P

E

E

A

N

M

L

D

K

S

D

L

L

K

I

K

A

A

K

V

V

L

K

E

A

N

E

K

N

A

A

D

D

I

L

G

G

I

L

I

A

F

F

D
|
D

T

G

D
|
D

V

G

D
|
D

R
|
R

A

V

A

G

I

V

V

V

D

T

S

N

Y

T

G

G

R

T

E

I

I

I

N

Y

R

P

N

D

L

R

L

L

I

L

A

M

L

L

T

F

S

A

A

K

I

D

V

V

L

V

E

S

E

R

H

N

P

P

G

G

S

A

I

D

I

I

V

I

T

F

D

D

S

V

V
x
K

T

C

S

T

N

R

G

R

L

L

K

I

K

A

F

L

I

I

E

S

K

G

R

Y

G

G

I

R

H

P

H

V

R

M

F

W

K

K

R

T

G
|
G

Y
x
H

R

-

N

-

V

-

I

-

N

-

E

S

A

L

I

I

K

K

F

K

N

K

N

M

E

K

S

E

R

T

E

G

C

A

H

L

F

L

A

A

I

G

E
|
E

T

M

S
|
S

G

G

H
|
H

A

V

A

F

L

F

K

K

E

E

N

R

Y

W

F

F

-

G

L

F

D
|
D

D

D

G

G

A

I

Y

Y

L

S

V

A

C

A

K

R

I

L

L

L

I

E

K

I

M

L

A

S

R

Q

L

D

K

Q

N

R

D

D

S

S

N

E

G

H

K

V

L

F

S

S

D

A

L

F

I

P

S

S

D

D

L

I

D

S

S

T

P

P

C

-

E

-

S

-

K

-

E

-

Y

-

R

E

M

I

K

N

I

I

K

T

F

V

N

T

D

E

F

D

K

S

E

K

Y

F

G

A

S

-

N

-

I

I

L

I

D

E

N

A

L

L

K

Q

D

R

Y

D

V

A

K

Q

S

-

I

-

N

-

G

-

W

W

G

G

-

E

I

G

E

N

P

I

E

T

N

T

Y

L

E

D

G

G

I

V

K

R

V

V

N

D

C

Y

N

P

K

K

E

-

N

-

G

-

D

-

G

G

W

W

F

G

L

L

L

V

R
|
R

L

A

S
|
S

L
x
N

H
x
T

E

T

A

P

V

V

M

L

P

V

L

L

N

R

I

F

E

E

S

A

D

D

S

T

Q

E

G

E

V

L

E

E

F

R

I

I

active site: S100 (= S116), H101 (= H117), K110 (= K126), D234 (= D264), D236 (= D266), D238 (= D268), R239 (= R269), H321 (= H356), D332 (= D366)
binding 6-O-phosphono-alpha-D-glucopyranose: S100 (= S116), K277 (≠ V307), G299 (= G329), H300 (≠ Y330), E317 (= E352), S319 (= S354), H321 (= H356), R413 (= R462), S415 (= S464), N416 (≠ L465), T417 (≠ H466)
binding zinc ion: S100 (= S116), D234 (= D264), D236 (= D266), D238 (= D268)

Sites not aligning to the query:

active site: 12
binding 6-O-phosphono-alpha-D-glucopyranose: 9

1p5dX Enzyme-ligand complex of p. Aeruginosa pmm/pgm (see paper)
28% identity, 84% coverage: 57:485/508 of query aligns to 43:436/455 of 1p5dX

query
sites
1p5dX

I

V

S

A

I

V

G

G

M

R

D

D

S

G

R

R

I

L

T

S

S

G

E

P

S

E

I

L

K

V

E

K

I

Q

L

L

I

I

S

Q

E

G

I

L

K

V

K

D

L

C

G

G

C

C

T

Q

V

V

M

S

D

D

C

V

G

G

L

M

C

V

S

P

T

T

P

P

A

V

M

L

F

Y

M

Y

S

A

T

A

I

N

F

V

E

L

N

E

Y

G

K

K

S

S

D

-

G

-

A

G

I

V

E

M

I

L

T

T

A

G

S
|
S

H
|
H

L

N

P

P

Y

P

Y

D

Y

Y

N

N

G

G

F

F

K
|
K

F

I

F

V

T

V

N

A

K

G

G

E

G

T

F

L

D

A

K

N

E

E

D

Q

I

I

N

Q

E

A

L

L

L

R

D

E

I

-

A

-

Q

R

N

I

E

E

K

K

V

N

C

D

T

L

V

A

Q

S

K

G

L

V

G

G

A

S

I

V

I

E

K

Q

C

V

D

D

L

I

M

L

K

P

D

R

Y

Y

S

-

N

-

F

-

L

-

I

-

N

-

K

-

I

-

I

F

D

K

G

Q

I

I

D

R

D

D

K

D

E

I

H

A

R

M

L

A

K

K

P

P

L

M

E

-

N

-

F

-

K

K

I

V

V

V

D

D

A

C

G

G

N

N

G

G

A

V

G

A

G

G

F

V

F

I

V

A

E

P

K

Q

V

L

L

I

N

E

Q

A

L

L

G

G

A

C

D

S

T

V

N

I

G

-

S

P

Q

L

F

Y

I

C

E

E

P

V

D

D

G

G

T

N

F

F

P

P

N

N

H

H

I

H

P

P

N

D

P

P

E

G

K

K

K

P

E

E

A

N

M

L

D

K

S

D

L

L

K

I

K

A

A

K

V

V

L

K

E

A

N

E

K

N

A

A

D

D

I

L

G

G

I

L

I

A

F

F

D
|
D

T

G

D
|
D

V

G

D
|
D

R
|
R

A

V

A

G

I

V

V

V

D

T

S

N

Y

T

G

G

R

T

E

I

I

I

N

Y

R

P

N

D

L

R

L

L

I

L

A

M

L

L

T

F

S

A

A

K

I

D

V

V

L

V

E

S

E

R

H

N

P

P

G

G

S

A

I

D

I

I

V

I

T

F

D

D

S

V

V

K

T

C

S

T

N

R

G

R

L

L

K

I

K

A

F

L

I

I

E

S

K

G

R

Y

G

G

I

R

H

P

H

V

R

M

F

W

K

K

R
x
T

G
|
G

Y
x
H

R

-

N

-

V

-

I

-

N

-

E

S

A

L

I

I

K

K

F

K

N

K

N

M

E

K

S

E

R

T

E

G

C

A

H

L

F

L

A

A

I

G

E
|
E

T

M

S
|
S

G

G

H
|
H

A

V

A

F

L

F

K

K

E

E

N

R

Y

W

F

F

-

G

L

F

D
|
D

D

D

G

G

A

I

Y

Y

L

S

V

A

C

A

K

R

I

L

L

L

I

E

K

I

M

L

A

S

R

Q

L

D

K

Q

N

R

D

D

S

S

N

E

G

H

K

V

L

F

S

S

D

A

L

F

I

P

S

S

D

D

L

I

D

S

S

T

P

P

C

-

E

-

S

-

K

-

E

-

Y

-

R

E

M

I

K

N

I

I

K

T

F

V

N

T

D

E

F

D

K

S

E

K

Y

F

G

A

S

-

N

-

I

I

L

I

D

E

N

A

L

L

K

Q

D

R

Y

D

V

A

K

Q

S

-

I

-

N

-

G

-

W

W

G

G

-

E

I

G

E

N

P

I

E

T

N

T

Y

L

E

D

G

G

I

V

K

R

V

V

N

D

C

Y

N

P

K

K

E

-

N

-

G

-

D

-

G

G

W

W

F

G

L

L

L

V

R
|
R

L

A

S
|
S

L

N

H
x
T

E

T

A

P

V

V

M

L

P

V

L

L

N

R

I

F

E

E

S

A

D

D

S

T

Q

E

G

E

V

L

E

E

F

R

I

I

active site: S100 (= S116), H101 (= H117), K110 (= K126), D234 (= D264), D236 (= D266), D238 (= D268), R239 (= R269), H321 (= H356), D332 (= D366)
binding 1-O-phosphono-alpha-D-glucopyranose: S100 (= S116), R239 (= R269), T298 (≠ R328), G299 (= G329), H300 (≠ Y330), E317 (= E352), S319 (= S354), H321 (= H356), R413 (= R462), S415 (= S464), T417 (≠ H466)
binding zinc ion: S100 (= S116), D234 (= D264), D236 (= D266), D238 (= D268)

Sites not aligning to the query:

active site: 12
binding 1-O-phosphono-alpha-D-glucopyranose: 9

1pcjX Enzyme-ligand complex of p. Aeruginosa pmm/pgm (see paper)
28% identity, 84% coverage: 57:485/508 of query aligns to 46:439/458 of 1pcjX

query
sites
1pcjX

I

V

S

A

I

V

G

G

M

R

D

D

S

G

R

R

I

L

T

S

S

G

E

P

S

E

I

L

K

V

E

K

I

Q

L

L

I

I

S

Q

E

G

I

L

K

V

K

D

L

C

G

G

C

C

T

Q

V

V

M

S

D

D

C

V

G

G

L

M

C

V

S

P

T

T

P

P

A

V

M

L

F

Y

M

Y

S

A

T

A

I

N

F

V

E

L

N

E

Y

G

K

K

S

S

D

-

G

-

A

G

I

V

E

M

I

L

T

T

A

G

S
|
S

H
|
H

L

N

P

P

Y

P

Y

D

Y

Y

N

N

G

G

F

F

K
|
K

F

I

F

V

T

V

N

A

K

G

G

E

G

T

F

L

D

A

K

N

E

E

D

Q

I

I

N

Q

E

A

L

L

L

R

D

E

I

-

A

-

Q

R

N

I

E

E

K

K

V

N

C

D

T

L

V

A

Q

S

K

G

L

V

G

G

A

S

I

V

I

E

K

Q

C

V

D

D

L

I

M

L

K

P

D

R

Y

Y

S

-

N

-

F

-

L

-

I

-

N

-

K

-

I

-

I

F

D

K

G

Q

I

I

D

R

D

D

K

D

E

I

H

A

R

M

L

A

K

K

P

P

L

M

E

-

N

-

F

-

K

K

I

V

V

V

D

D

A

C

G

G

N

N

G

G

A

V

G

A

G

G

F

V

F

I

V

A

E

P

K

Q

V

L

L

I

N

E

Q

A

L

L

G

G

A

C

D

S

T

V

N

I

G

-

S

P

Q

L

F

Y

I

C

E

E

P

V

D

D

G

G

T

N

F

F

P

P

N

N

H

H

I

H

P

P

N

D

P

P

E

G

K

K

K

P

E

E

A

N

M

L

D

K

S

D

L

L

K

I

K

A

A

K

V

V

L

K

E

A

N

E

K

N

A

A

D

D

I

L

G

G

I

L

I

A

F

F

D
|
D

T

G

D
|
D

V

G

D
|
D

R
|
R

A

V

A

G

I

V

V

V

D

T

S

N

Y

T

G

G

R

T

E

I

I

I

N

Y

R

P

N

D

L

R

L

L

I

L

A

M

L

L

T

F

S

A

A

K

I

D

V

V

L

V

E

S

E

R

H

N

P

P

G

G

S

A

I

D

I

I

V

I

T

F

D

D

S

V

V

K

T

C

S

T

N

R

G

R

L

L

K

I

K

A

F

L

I

I

E

S

K

G

R

Y

G

G

I

R

H

P

H

V

R

M

F

W

K

K

R
x
T

G
|
G

Y

H

R

-

N

-

V

-

I

-

N

-

E

S

A

L

I

I

K

K

F

K

N

K

N

M

E

K

S

E

R

T

E

G

C

A

H

L

F

L

A

A

I

G

E
|
E

T

M

S
|
S

G

G

H
|
H

A

V

A

F

L

F

K

K

E

E

N

R

Y

W

F

F

-

G

L

F

D
|
D

D

D

G

G

A

I

Y

Y

L

S

V

A

C

A

K

R

I

L

L

L

I

E

K

I

M

L

A

S

R

Q

L

D

K

Q

N

R

D

D

S

S

N

E

G

H

K

V

L

F

S

S

D

A

L

F

I

P

S

S

D

D

L

I

D

S

S

T

P

P

C

-

E

-

S

-

K

-

E

-

Y

-

R

E

M

I

K

N

I

I

K

T

F

V

N

T

D

E

F

D

K

S

E

K

Y

F

G

A

S

-

N

-

I

I

L

I

D

E

N

A

L

L

K

Q

D

R

Y

D

V

A

K

Q

S

-

I

-

N

-

G

-

W

W

G

G

-

E

I

G

E

N

P

I

E

T

N

T

Y

L

E

D

G

G

I

V

K

R

V

V

N

D

C

Y

N

P

K

K

E

-

N

-

G

-

D

-

G

G

W

W

F

G

L

L

L

V

R
|
R

L

A

S
|
S

L
x
N

H
x
T

E

T

A

P

V

V

M

L

P

V

L

L

N

R

I

F

E

E

S

A

D

D

S

T

Q

E

G

E

V

L

E

E

F

R

I

I

active site: S103 (= S116), H104 (= H117), K113 (= K126), D237 (= D264), D239 (= D266), D241 (= D268), R242 (= R269), H324 (= H356), D335 (= D366)
binding 1-O-phosphono-alpha-D-mannopyranose: S103 (= S116), T301 (≠ R328), G302 (= G329), E320 (= E352), S322 (= S354), H324 (= H356), R416 (= R462), S418 (= S464), N419 (≠ L465), T420 (≠ H466)
binding zinc ion: S103 (= S116), D237 (= D264), D239 (= D266), D241 (= D268)

Sites not aligning to the query:

active site: 15
binding 1-O-phosphono-alpha-D-mannopyranose: 12

1wqaA Crystal structure of pyrococcus horikoshii phosphomannomutase/phosphoglucomutase complexed with mg2+
30% identity, 87% coverage: 36:479/508 of query aligns to 26:436/455 of 1wqaA

query
sites
1wqaA

I

I

A

G

K

M

A

A

F

F

V

G

L

T

L

L

S

K

R

R

K

E

K

G

N

R

R

K

D

-

L

-

S

-

K

K

L

P

K

L

I

V

S

V

I

V

G

G

M

R

D

D

S

T

R

R

I

V

T

S

S

G

E

E

S

M

I

L

K

K

E

E

I

A

L

L

I

I

S

S

E

G

I

L

K

L

K

S

L

V

G

G

C

C

T

D

V

V

M

I

D

D

C

V

G

G

L

I

C

A

S

P

T

T

P

P

A

A

M

V

F

Q

M

W

S

A

T

T

I

-

F

-

E

K

N

H

Y

F

K

N

S

A

D

D

G

G

A

G

I

A

E

V

I

I

T

T

A

A

S
|
S

H
|
H

L

N

P

P

Y

P

Y

E

Y

Y

N

N

G

G

F

I

K
|
K

F

L

T

E

N

P

K

N

G

G

-

M

G

G

F

L

-

K

D

E

K

R

E

E

D

A

I

I

N

V

E

E

L

E

L

L

D

F

I

F

A

K

Q

E

N

D

E

F

K

D

V

R

C

A

T

K

V

W

Q

Y

K

E

L

I

G

G

A

E

I

V

I

R

K

R

C

E

D

D

L

I

M

I

K

K

D

P

Y

Y

S

I

N

E

F

A

L

I

I

K

N

S

K

K

I

V

-

D

I

V

D

E

G

A

I

I

D

K

K

R

E

-

H

-

R

-

L

-

K

K

P

P

L

F

E

-

N

-

F

-

K

-

I

V

V

V

D

D

A

T

G

S

N

N

G

G

A

A

G

G

G

S

F

L

F

T

V

L

E

P

K

Y

V

L

L

L

N

R

Q

E

L

L

G

G

A

C

D

K

T

V

N

I

G

-

S

T

Q

V

F

N

I

A

E

Q

P

P

D

D

G

G

T

Y

F

F

P

P

N

A

H

R

I

N

P

P

N

E

P

P

E

-

K

N

K

E

E

E

A

N

M

L

D

K

S

E

L

F

K

M

K

E

A

I

V

V

L

K

E

A

N

L

K

G

A

A

D

D

I

F

G

G

I

V

I

A

F

Q

D
|
D

T

G

D
|
D

V

A

D
|
D

R
|
R

A

A

A

V

I

F

V

I

D

D

S

E

Y

N

G

G

R

R

E

F

I

I

N

Q

R

G

N

D

L

K

L

T

I

F

A

A

L

L

T

V

S

A

A

D

I

A

V

V

L

L

E

K

E

E

H

K

P

G

G

G

S

G

I

L

V

V

T

T

D

T

S

V

V

A

T

T

S

S

N

N

G

L

L

L

K

D

F

I

I

A

E

K

K

K

R

H

G

G

G

A

I

K

H

V

H

M

R

R

F

T

K

K

R

V

G

G

Y

-

R

D

N

L

V

I

I

V

N

A

E

R

A

A

I

L

K

Y

F

E

N

N

E

G

S

T

R

-

E

-

C

-

H

-

F

I

A

G

I

G

E

E

T

E

S

N

G

G

H
x
G

A

V

A

I

L

F

K

P

E

E

N

H

Y

V

F

L

L

G

D
x
R

D

D

G

G

A

A

Y

M

L

T

V

V

C

A

K

K

I

V

L

V

I

E

K

I

M

F

A

A

R

K

L

-

K

-

N

-

D

-

S

S

N

G

G

K

K

K

L

F

S

S

D

E

L

L

I

I

S

D

D

E

L

L

D

-

S

-

P

-

C

-

E

-

S

P

K

K

E

Y

Y

Y

R

Q

M

I

K

K

I

T

K

K

F

R

N

H

D

V

F

E

K

G

E

D

Y

R

G

H

S

A

N

-

I

I

L

V

D

N

N

K

L

V

K

A

D

E

Y

M

V

A

K

R

S

E

I

-

N

R

G

G

W

Y

G

T

I

V

E

-

P

-

E

D

N

T

Y

T

E

D

G

G

I

A

K

K

V

I

N

I

C

F

N

E

K

-

E

-

N

-

G

-

D

D

G

G

W

W

F

V

L

L

L

V

R

R

L

A

S

S

L

G

H

T

E

E

A

P

V

I

M

I

P

R

L

I

N

F

I

S

E

E

S

A

D

K

S

S

Q

K

active site: S101 (= S116), H102 (= H117), K111 (= K126), D243 (= D264), D245 (= D266), D247 (= D268), R248 (= R269), G330 (≠ H356), R340 (≠ D366)
binding magnesium ion: S101 (= S116), D243 (= D264), D245 (= D266), D247 (= D268)

Sites not aligning to the query:

active site: 11

1k2yX Crystal structure of phosphomannomutase/phosphoglucomutase s108a mutant from p. Aeruginosa (see paper)
28% identity, 84% coverage: 57:485/508 of query aligns to 47:440/459 of 1k2yX

query
sites
1k2yX

I

V

S

A

I

V

G

G

M

R

D

D

S

G

R

R

I

L

T

S

S

G

E

P

S

E

I

L

K

V

E

K

I

Q

L

L

I

I

S

Q

E

G

I

L

K

V

K

D

L

C

G

G

C

C

T

Q

V

V

M

S

D

D

C

V

G

G

L

M

C

V

S

P

T

T

P

P

A

V

M

L

F

Y

M

Y

S

A

T

A

I

N

F

V

E

L

N

E

Y

G

K

K

S

S

D

-

G

-

A

G

I

V

E

M

I

L

T

T

A

G

S

A

H
|
H

L

N

P

P

Y

P

Y

D

Y

Y

N

N

G

G

F

F

K

K

F

I

F

V

T

V

N

A

K

G

G

E

G

T

F

L

D

A

K

N

E

E

D

Q

I

I

N

Q

E

A

L

L

L

R

D

E

I

-

A

-

Q

R

N

I

E

E

K

K

V

N

C

D

T

L

V

A

Q

S

K

G

L

V

G

G

A

S

I

V

I

E

K

Q

C

V

D

D

L

I

M

L

K

P

D

R

Y

Y

S

-

N

-

F

-

L

-

I

-

N

-

K

-

I

-

I

F

D

K

G

Q

I

I

D

R

D

D

K

D

E

I

H

A

R

M

L

A

K

K

P

P

L

M

E

-

N

-

F

-

K

K

I

V

V

V

D

D

A

C

G

G

N

N

G

G

A

V

G

A

G

G

F

V

F

I

V

A

E

P

K

Q

V

L

L

I

N

E

Q

A

L

L

G

G

A

C

D

S

T

V

N

I

G

-

S

P

Q

L

F

Y

I

C

E

E

P

V

D

D

G

G

T

N

F

F

P

P

N

N

H

H

I

H

P

P

N

D

P

P

E

G

K

K

K

P

E

E

A

N

M

L

D

K

S

D

L

L

K

I

K

A

A

K

V

V

L

K

E

A

N

E

K

N

A

A

D

D

I

L

G

G

I

L

I

A

F

F

D
|
D

T

G

D
|
D

V

G

D
|
D

R
|
R

A

V

A

G

I

V

V

V

D

T

S

N

Y

T

G

G

R

T

E

I

I

I

N

Y

R

P

N

D

L

R

L

L

I

L

A

M

L

L

T

F

S

A

A

K

I

D

V

V

L

V

E

S

E

R

H

N

P

P

G

G

S

A

I

D

I

I

V

I

T

F

D

D

S

V

V

K

T

C

S

T

N

R

G

R

L

L

K

I

K

A

F

L

I

I

E

S

K

G

R

Y

G

G

I

R

H

P

H

V

R

M

F

W

K

K

R

T

G

G

Y

H

R

-

N

-

V

-

I

-

N

-

E

S

A

L

I

I

K

K

F

K

N

K

N

M

E

K

S

E

R

T

E

G

C

A

H

L

F

L

A

A

I

G

E

E

T

M

S

S

G

G

H

H

A

V

A

F

L

F

K

K

E

E

N

R

Y

W

F

F

-

G

L

F

D
|
D

D

D

G

G

A

I

Y

Y

L

S

V

A

C

A

K

R

I

L

L

L

I

E

K

I

M

L

A

S

R

Q

L

D

K

Q

N

R

D

D

S

S

N

E

G

H

K

V

L

F

S

S

D

A

L

F

I

P

S

S

D

D

L

I

D

S

S

T

P

P

C

-

E

-

S

-

K

-

E

-

Y

-

R

E

M

I

K

N

I

I

K

T

F

V

N

T

D

E

F

D

K

S

E

K

Y

F

G

A

S

-

N

-

I

I

L

I

D

E

N

A

L

L

K

Q

D

R

Y

D

V

A

K

Q

S

-

I

-

N

-

G

-

W

W

G

G

-

E

I

G

E

N

P

I

E

T

N

T

Y

L

E

D

G

G

I

V

K

R

V

V

N

D

C

Y

N

P

K

K

E

-

N

-

G

-

D

-

G

G

W

W

F

G

L

L

L

V

R

R

L

A

S

S

L

N

H

T

E

T

A

P

V

V

M

L

P

V

L

L

N

R

I

F

E

E

S

A

D

D

S

T

Q

E

G

E

V

L

E

E

F

R

I

I

active site: H105 (= H117), D238 (= D264), D240 (= D266), D242 (= D268), R243 (= R269), D336 (= D366)
binding zinc ion: D238 (= D264), D240 (= D266), D242 (= D268)

4il8A Crystal structure of an h329a mutant of p. Aeruginosa pmm/pgm (see paper)
28% identity, 84% coverage: 57:485/508 of query aligns to 47:440/459 of 4il8A

query
sites
4il8A

I

V

S

A

I

V

G

G

M

R

D

D

S

G

R

R

I

L

T

S

S

G

E

P

S

E

I

L

K

V

E

K

I

Q

L

L

I

I

S

Q

E

G

I

L

K

V

K

D

L

C

G

G

C

C

T

Q

V

V

M

S

D

D

C

V

G

G

L

M

C

V

S

P

T

T

P

P

A

V

M

L

F

Y

M

Y

S

A

T

A

I

N

F

V

E

L

N

E

Y

G

K

K

S

S

D

-

G

-

A

G

I

V

E

M

I

L

T

T

A

G

S
|
S

H
|
H

L

N

P

P

Y

P

Y

D

Y

Y

N

N

G

G

F

F

K
|
K

F

I

F

V

T

V

N

A

K

G

G

E

G

T

F

L

D

A

K

N

E

E

D

Q

I

I

N

Q

E

A

L

L

L

R

D

E

I

-

A

-

Q

R

N

I

E

E

K

K

V

N

C

D

T

L

V

A

Q

S

K

G

L

V

G

G

A

S

I

V

I

E

K

Q

C

V

D

D

L

I

M

L

K

P

D

R

Y

Y

S

-

N

-

F

-

L

-

I

-

N

-

K

-

I

-

I

F

D

K

G

Q

I

I

D

R

D

D

K

D

E

I

H

A

R

M

L

A

K

K

P

P

L

M

E

-

N

-

F

-

K

K

I

V

V

V

D

D

A

C

G

G

N

N

G

G

A

V

G

A

G

G

F

V

F

I

V

A

E

P

K

Q

V

L

L

I

N

E

Q

A

L

L

G

G

A

C

D

S

T

V

N

I

G

-

S

P

Q

L

F

Y

I

C

E

E

P

V

D

D

G

G

T

N

F

F

P

P

N

N

H

H

I

H

P

P

N

D

P

P

E

G

K

K

K

P

E

E

A

N

M

L

D

K

S

D

L

L

K

I

K

A

A

K

V

V

L

K

E

A

N

E

K

N

A

A

D

D

I

L

G

G

I

L

I

A

F

F

D
|
D

T

G

D
|
D

V

G

D
|
D

R
|
R

A

V

A

G

I

V

V

V

D

T

S

N

Y

T

G

G

R

T

E

I

I

I

N

Y

R

P

N

D

L

R

L

L

I

L

A

M

L

L

T

F

S

A

A

K

I

D

V

V

L

V

E

S

E

R

H

N

P

P

G

G

S

A

I

D

I

I

V

I

T

F

D

D

S

V

V

K

T

C

S

T

N

R

G

R

L

L

K

I

K

A

F

L

I

I

E

S

K

G

R

Y

G

G

I

R

H

P

H

V

R

M

F

W

K

K

R

T

G

G

Y

H

R

-

N

-

V

-

I

-

N

-

E

S

A

L

I

I

K

K

F

K

N

K

N

M

E

K

S

E

R

T

E

G

C

A

H

L

F

L

A

A

I

G

E

E

T

M

S

S

G

G

H
x
A

A

V

A

F

L

F

K

K

E

E

N

R

Y

W

F

F

-

G

L

F

D
|
D

D

D

G

G

A

I

Y

Y

L

S

V

A

C

A

K

R

I

L

L

L

I

E

K

I

M

L

A

S

R

Q

L

D

K

Q

N

R

D

D

S

S

N

E

G

H

K

V

L

F

S

S

D

A

L

F

I

P

S

S

D

D

L

I

D

S

S

T

P

P

C

-

E

-

S

-

K

-

E

-

Y

-

R

E

M

I

K

N

I

I

K

T

F

V

N

T

D

E

F

D

K

S

E

K

Y

F

G

A

S

-

N

-

I

I

L

I

D

E

N

A

L

L

K

Q

D

R

Y

D

V

A

K

Q

S

-

I

-

N

-

G

-

W

W

G

G

-

E

I

G

E

N

P

I

E

T

N

T

Y

L

E

D

G

G

I

V

K

R

V

V

N

D

C

Y

N

P

K

K

E

-

N

-

G

-

D

-

G

G

W

W

F

G

L

L

L

V

R

R

L

A

S

S

L

N

H

T

E

T

A

P

V

V

M

L

P

V

L

L

N

R

I

F

E

E

S

A

D

D

S

T

Q

E

G

E

V

L

E

E

F

R

I

I

active site: S104 (= S116), H105 (= H117), K114 (= K126), D238 (= D264), D240 (= D266), D242 (= D268), R243 (= R269), A325 (≠ H356), D336 (= D366)
binding magnesium ion: S104 (= S116), D238 (= D264), D240 (= D266), D242 (= D268)

Sites not aligning to the query:

active site: 16

6nqhA Xanthomonas citri dephospho-pgm in complex with xylose-1-phosphate
30% identity, 84% coverage: 52:476/508 of query aligns to 35:428/448 of 6nqhA

query
sites
6nqhA

L

L

S

D

K

Q

L

G

K

P

I

V

S

V

I

L

G

G

M

H

D

D

S

V

R

R

I

L

T

A

S

S

E

P

S

A

I

L

K

Q

E

E

I

A

L

L

I

S

S

A

E

G

I

L

K

R

K

A

L

S

G

G

C

R

T

D

V

V

M

I

D

D

C

I

G

G

L

L

C

C

S

G

T

T

P

E

A

E

M

V

F

Y

M

F

S

Q

T

T

I

D

F

Y

E

-

N

-

Y

L

K

K

S

A

D

A

G

G

A

G

I

V

E

M

I

V

T

T

A

A

S
|
S

H
|
H

L

N

P

P

Y

M

Y

D

Y

Y

N

N

G

G

F

M

K
|
K

F

L

F

V

-

R

-

E

-

Q

-

A

-

R

-

P

-

I

-

S

T

S

N

D

K

T

G

G

G

L

F

F

D

A

K

I

E

R

D

D

I

T

N

V

E

A

L

A

L

D

D

T

I

A

A

A

Q

P

N

G

E

E

K

P

V

T

C

A

T

S

V

E

Q

Q

K

S

L

-

G

-

A

-

I

-

K

-

C

-

D

-

L

-

M

R

K

T

D

D

Y

K

S

T

N

A

F

Y

L

L

I

-

N

E

K

H

I

L

D

S

G

Y

I

V

D

D

D

-

K

-

E

R

H

S

R

T

L

L

K

K

P

P

L

L

E

-

N

-

F

-

K

K

I

L

V

V

D

N

A

A

G

G

N

N

G

G

A

G

G

A

G

G

F

L

F

I

V

V

E

D

K

L

V

L

L

A

N

P

Q

H

L

L

G

P

A

F

D

E

T

F

N

V

G

-

S

R

Q

V

F

F

I

H

E

E

P

P

D

D

G

G

T

N

F

F

P

P

N

N

H

G

I

I

P

P

N

N

P

P

E

L

K

L

K

P

E

E

A

N

M

R

D

D

S

A

L

T

K

A

K

K

A

A

V

V

L

K

E

D

N

N

K

G

A

A

D

D

I

F

G

G

I

I

I

A

F

W

D
|
D

T

G

D
|
D

V

F

D
|
D

R
|
R

A

C

A

F

I

F

V

F

D

D

S

H

Y

T

G

G

R

R

E

F

I

I

N

E

R

G

N

Y

L

Y

L

L

I

V

A

G

L

L

T

L

S

A

A

Q

I

A

V

I

L

L

E

A

E

K

H

Q

P

P

G

G

S

G

I

K

I

V

V

V

T

H

D

D

S

P

V
x
R

T

L

S

T

N

W

G

N

L

T

K

V

K

E

F

Q

I

V

E

E

K

E

R

A

G

G

I

I

H

P

H

V

R

L

F

C

K

K

R
x
S

G
|
G

Y

H

R

-

N

-

V

-

I

-

N

-

E

A

A

F

I

I

K

K

F

E

N

K

N

M

E

R

S

S

R

E

E

N

C

A

H

V

F

Y

A

G

I

G

E
|
E

T

M

S
|
S

G

A

H
|
H

A

H

A

Y

L

F

K

R

E

E

N

F

Y

A

F

Y

L

A

D

D

D

S

G

G

A

-

Y

-

L

M

V

I

C

P

K

W

I

L

L

L

I

I

K

-

M

-

A

A

R

E

L

L

K

V

N

S

D

Q

S

S

N

G

G

R

K

S

L

L

S

A

D

D

L

L

I

V

S

-

D

-

L

-

D

-

S

-

P

-

C

-

E

-

S

-

K

-

E

E

Y

A

R

R

M

M

K

Q

I

-

K

K

F

F

N

P

D

C

F

S

K

G

E

E

Y

I

G

N

S

F

N

K

I

V

L

A

D

D

N

A

L

K

K

A

D

S

Y

V

V

A

K

R

S

V

I

M

N

E

G

H

W

Y

G

A

-

S

I

L

E

S

P

P

E

E

-

L

N

D

Y

Y

-

T

E

D

G

G

I

I

K

S

V

A

N

D

C

F

N

G

K

Q

E

-

N

-

G

-

D

-

G

-

W

W

-

R

F

F

L

N

L

L

R
|
R

L

S

S
|
S

L
x
N

H
x
T

E

E

A

P

V

L

M

L

P
x
R

L

L

N

N

I

V

E

E

S

T

active site: S97 (= S116), H98 (= H117), K107 (= K126), D237 (= D264), D239 (= D266), D241 (= D268), R242 (= R269), H324 (= H356)
binding magnesium ion: D237 (= D264), D239 (= D266), D241 (= D268)
binding 1-O-phosphono-alpha-D-xylopyranose: S97 (= S116), H98 (= H117), K107 (= K126), D239 (= D266), R242 (= R269), R280 (≠ V307), S301 (≠ R328), G302 (= G329), E320 (= E352), S322 (= S354), H324 (= H356), R414 (= R462), S416 (= S464), N417 (≠ L465), T418 (≠ H466), R423 (≠ P471)

Sites not aligning to the query:

active site: 12
binding 1-O-phosphono-alpha-D-xylopyranose: 12

6np8A Xanthomonas citri phospho-pgm in complex with mannose-6-phosphate (see paper)
30% identity, 84% coverage: 52:476/508 of query aligns to 35:428/448 of 6np8A

query
sites
6np8A

L

L

S

D

K

Q

L

G

K

P

I

V

S

V

I

L

G

G

M

H

D

D

S

V

R

R

I

L

T

A

S

S

E

P

S

A

I

L

K

Q

E

E

I

A

L

L

I

S

S

A

E

G

I

L

K

R

K

A

L

S

G

G

C

R

T

D

V

V

M

I

D

D

C

I

G

G

L

L

C

C

S

G

T

T

P

E

A

E

M

V

F

Y

M

F

S

Q

T

T

I

D

F

Y

E

-

N

-

Y

L

K

K

S

A

D

A

G

G

A

G

I

V

E

M

I

V

T

T

A

A

S
|
S

H
|
H

L

N

P

P

Y

M

Y

D

Y

Y

N

N

G

G

F

M

K
|
K

F

L

F

V

-

R

-

E

-

Q

-

A

-

R

-

P

-

I

-

S

T

S

N

D

K

T

G

G

G

L

F

F

D

A

K

I

E

R

D

D

I

T

N

V

E

A

L

A

L

D

D

T

I

A

A

A

Q

P

N

G

E

E

K

P

V

T

C

A

T

S

V

E

Q

Q

K

S

L

-

G

-

A

-

I

-

K

-

C

-

D

-

L

-

M

R

K

T

D

D

Y

K

S

T

N

A

F

Y

L

L

I

-

N

E

K

H

I

L

D

S

G

Y

I

V

D

D

D

-

K

-

E

R

H

S

R

T

L

L

K

K

P

P

L

L

E

-

N

-

F

-

K

K

I

L

V

V

D

N

A

A

G

G

N

N

G

G

A

G

G

A

G

G

F

L

F

I

V

V

E

D

K

L

V

L

L

A

N

P

Q

H

L

L

G

P

A

F

D

E

T

F

N

V

G

-

S

R

Q

V

F

F

I

H

E

E

P

P

D

D

G

G

T

N

F

F

P

P

N

N

H

G

I

I

P

P

N

N

P

P

E

L

K

L

K

P

E

E

A

N

M

R

D

D

S

A

L

T

K

A

K

K

A

A

V

V

L

K

E

D

N

N

K

G

A

A

D

D

I

F

G

G

I

I

I

A

F

W

D
|
D

T

G

D
|
D

V

F

D
|
D

R
|
R

A

C

A

F

I

F

V

F

D

D

S

H

Y

T

G

G

R

R

E

F

I

I

N

E

R

G

N

Y

L

Y

L

L

I

V

A

G

L

L

T

L

S

A

A

Q

I

A

V

I

L

L

E

A

E

K

H

Q

P

P

G

G

S

G

I

K

I

V

V

V

T

H

D

D

S

P

V
x
R

T

L

S

T

N

W

G

N

L

T

K

V

K

E

F

Q

I

V

E

E

K

E

R

A

G

G

I

I

H

P

H

V

R

L

F

C

K

K

R

S

G
|
G

Y
x
H

R

-

N

-

V

-

I

-

N

-

E

A

A

F

I

I

K

K

F

E

N

K

N

M

E

R

S

S

R

E

E

N

C

A

H

V

F

Y

A

G

I

G

E
|
E

T

M

S
|
S

G

A

H
|
H

A

H

A

Y

L

F

K

R

E

E

N

F

Y

A

F

Y

L

A

D

D

D

S

G

G

A

-

Y

-

L

M

V

I

C

P

K

W

I

L

L

L

I

I

K

-

M

-

A

A

R

E

L

L

K

V

N

S

D

Q

S

S

N

G

G

R

K

S

L

L

S

A

D

D

L

L

I

V

S

-

D

-

L

-

D

-

S

-

P

-

C

-

E

-

S

-

K

-

E

E

Y

A

R

R

M

M

K

Q

I

-

K

K

F

F

N

P

D

C

F

S

K

G

E

E

Y

I

G

N

S

F

N

K

I

V

L

A

D

D

N

A

L

K

K

A

D

S

Y

V

V

A

K

R

S

V

I

M

N

E

G

H

W

Y

G

A

-

S

I

L

E

S

P

P

E

E

-

L

N

D

Y

Y

-

T

E

D

G

G

I

I

K

S

V

A

N

D

C

F

N

G

K

Q

E

-

N

-

G

-

D

-

G

-

W

W

-

R

F

F

L

N

L

L

R
|
R

L

S

S
|
S

L
x
N

H
x
T

E

E

A

P

V

L

M

L

P
x
R

L

L

N

N

I

V

E

E

S

T

active site: S97 (= S116), H98 (= H117), K107 (= K126), D237 (= D264), D239 (= D266), D241 (= D268), R242 (= R269), H324 (= H356)
binding calcium ion: S97 (= S116), D237 (= D264), D239 (= D266), D241 (= D268)
binding 6-O-phosphono-alpha-D-mannopyranose: R280 (≠ V307), G302 (= G329), H303 (≠ Y330), E320 (= E352), S322 (= S354), H324 (= H356), R414 (= R462), S416 (= S464), N417 (≠ L465), T418 (≠ H466), R423 (≠ P471)

Sites not aligning to the query:

active site: 12
binding 6-O-phosphono-alpha-D-mannopyranose: 9

6nolA Xanthomonas citri dephospho-pgm in complex with mannose-1-phosphate (see paper)
30% identity, 84% coverage: 52:476/508 of query aligns to 35:428/448 of 6nolA

query
sites
6nolA

L

L

S

D

K

Q

L

G

K

P

I

V

S

V

I

L

G

G

M

H

D

D

S

V

R

R

I

L

T

A

S

S

E

P

S

A

I

L

K

Q

E

E

I

A

L

L

I

S

S

A

E

G

I

L

K

R

K

A

L

S

G

G

C

R

T

D

V

V

M

I

D

D

C

I

G

G

L

L

C

C

S

G

T

T

P

E

A

E

M

V

F

Y

M

F

S

Q

T

T

I

D

F

Y

E

-

N

-

Y

L

K

K

S

A

D

A

G

G

A

G

I

V

E

M

I

V

T

T

A

A

S
|
S

H
|
H

L

N

P

P

Y

M

Y

D

Y

Y

N

N

G

G

F

M

K
|
K

F

L

F

V

-

R

-

E

-

Q

-

A

-

R

-

P

-

I

-

S

T

S

N

D

K

T

G

G

G

L

F

F

D

A

K

I

E

R

D

D

I

T

N

V

E

A

L

A

L

D

D

T

I

A

A

A

Q

P

N

G

E

E

K

P

V

T

C

A

T

S

V

E

Q

Q

K

S

L

-

G

-

A

-

I

-

K

-

C

-

D

-

L

-

M

R

K

T

D

D

Y

K

S

T

N

A

F

Y

L

L

I

-

N

E

K

H

I

L

D

S

G

Y

I

V

D

D

D

-

K

-

E

R

H

S

R

T

L

L

K

K

P

P

L

L

E

-

N

-

F

-

K

K

I

L

V

V

D

N

A

A

G

G

N

N

G

G

A

G

G

A

G

G

F

L

F

I

V

V

E

D

K

L

V

L

L

A

N

P

Q

H

L

L

G

P

A

F

D

E

T

F

N

V

G

-

S

R

Q

V

F

F

I

H

E

E

P

P

D

D

G

G

T

N

F

F

P

P

N

N

H

G

I

I

P

P

N

N

P

P

E

L

K

L

K

P

E

E

A

N

M

R

D

D

S

A

L

T

K

A

K

K

A

A

V

V

L

K

E

D

N

N

K

G

A

A

D

D

I

F

G

G

I

I

I

A

F

W

D
|
D

T

G

D
|
D

V

F

D
|
D

R
|
R

A

C

A

F

I

F

V

F

D

D

S

H

Y

T

G

G

R

R

E

F

I

I

N

E

R

G

N

Y

L

Y

L

L

I

V

A

G

L

L

T

L

S

A

A

Q

I

A

V

I

L

L

E

A

E

K

H

Q

P

P

G

G

S

G

I

K

I

V

V

V

T

H

D

D

S

P

V

R

T

L

S

T

N

W

G

N

L

T

K

V

K

E

F

Q

I

V

E

E

K

E

R

A

G

G

I

I

H

P

H

V

R

L

F

C

K

K

R

S

G
|
G

Y

H

R

-

N

-

V

-

I

-

N

-

E

A

A

F

I

I

K

K

F

E

N

K

N

M

E

R

S

S

R

E

E

N

C

A

H

V

F

Y

A

G

I

G

E
|
E

T

M

S
|
S

G

A

H
|
H

A

H

A

Y

L

F

K

R

E

E

N

F

Y

A

F

Y

L

A

D

D

D

S

G

G

A

-

Y

-

L

M

V

I

C

P

K

W

I

L

L

L

I

I

K

-

M

-

A

A

R

E

L

L

K

V

N

S

D

Q

S

S

N

G

G

R

K

S

L

L

S

A

D

D

L

L

I

V

S

-

D

-

L

-

D

-

S

-

P

-

C

-

E

-

S

-

K

-

E

E

Y

A

R

R

M

M

K

Q

I

-

K

K

F

F

N

P

D

C

F

S

K

G

E

E

Y

I

G

N

S

F

N

K

I

V

L

A

D

D

N

A

L

K

K

A

D

S

Y

V

V

A

K

R

S

V

I

M

N

E

G

H

W

Y

G

A

-

S

I

L

E

S

P

P

E

E

-

L

N

D

Y

Y

-

T

E

D

G

G

I

I

K

S

V

A

N

D

C

F

N

G

K

Q

E

-

N

-

G

-

D

-

G

-

W

W

-

R

F

F

L

N

L

L

R
|
R

L

S

S
|
S

L
x
N

H
x
T

E

E

A

P

V

L

M

L

P
x
R

L

L

N

N

I

V

E

E

S

T

active site: S97 (= S116), H98 (= H117), K107 (= K126), D237 (= D264), D239 (= D266), D241 (= D268), R242 (= R269), H324 (= H356)
binding 1-O-phosphono-alpha-D-mannopyranose: G302 (= G329), E320 (= E352), S322 (= S354), H324 (= H356), R414 (= R462), S416 (= S464), N417 (≠ L465), T418 (≠ H466), R423 (≠ P471)
binding magnesium ion: S97 (= S116), D237 (= D264), D239 (= D266), D241 (= D268)

Sites not aligning to the query:

active site: 12

6nnpA Xanthomonas citri dephospho-pgm in complex with glucose-6-phosphate (see paper)
30% identity, 84% coverage: 52:476/508 of query aligns to 35:428/448 of 6nnpA

query
sites
6nnpA

L

L

S

D

K

Q

L

G

K

P

I

V

S

V

I

L

G

G

M

H

D

D

S

V

R

R

I

L

T

A

S

S

E

P

S

A

I

L

K

Q

E

E

I

A

L

L

I

S

S

A

E

G

I

L

K

R

K

A

L

S

G

G

C

R

T

D

V

V

M

I

D

D

C

I

G

G

L

L

C

C

S

G

T

T

P

E

A

E

M

V

F

Y

M

F

S

Q

T

T

I

D

F

Y

E

-

N

-

Y

L

K

K

S

A

D

A

G

G

A

G

I

V

E

M

I

V

T

T

A

A

S
|
S

H
|
H

L

N

P

P

Y

M

Y

D

Y

Y

N

N

G

G

F

M

K
|
K

F

L

F

V

-

R

-

E

-

Q

-

A

-

R

-

P

-

I

-

S

T

S

N

D

K

T

G

G

G

L

F

F

D

A

K

I

E

R

D

D

I

T

N

V

E

A

L

A

L

D

D

T

I

A

A

A

Q

P

N

G

E

E

K

P

V

T

C

A

T

S

V

E

Q

Q

K

S

L

-

G

-

A

-

I

-

K

-

C

-

D

-

L

-

M

R

K

T

D

D

Y

K

S

T

N

A

F

Y

L

L

I

-

N

E

K

H

I

L

D

S

G

Y

I

V

D

D

D

-

K

-

E

R

H

S

R

T

L

L

K

K

P

P

L

L

E

-

N

-

F

-

K

K

I

L

V

V

D

N

A

A

G

G

N

N

G

G

A

G

G

A

G

G

F

L

F

I

V

V

E

D

K

L

V

L

L

A

N

P

Q

H

L

L

G

P

A

F

D

E

T

F

N

V

G

-

S

R

Q

V

F

F

I

H

E

E

P

P

D

D

G

G

T

N

F

F

P

P

N

N

H

G

I

I

P

P

N

N

P

P

E

L

K

L

K

P

E

E

A

N

M

R

D

D

S

A

L

T

K

A

K

K

A

A

V

V

L

K

E

D

N

N

K

G

A

A

D

D

I

F

G

G

I

I

I

A

F

W

D
|
D

T

G

D
|
D

V

F

D
|
D

R
|
R

A

C

A

F

I

F

V

F

D

D

S

H

Y

T

G

G

R

R

E

F

I

I

N

E

R

G

N

Y

L

Y

L

L

I

V

A

G

L

L

T

L

S

A

A

Q

I

A

V

I

L

L

E

A

E

K

H

Q

P

P

G

G

S

G

I

K

I

V

V

V

T

H

D

D

S

P

V
x
R

T

L

S

T

N

W

G

N

L

T

K

V

K

E

F

Q

I

V

E

E

K

E

R

A

G

G

I

I

H

P

H

V

R

L

F

C

K

K

R

S

G
|
G

Y
x
H

R

-

N

-

V

-

I

-

N

-

E

A

A

F

I

I

K

K

F

E

N

K

N

M

E

R

S

S

R

E

E

N

C

A

H

V

F

Y

A

G

I

G

E
|
E

T

M

S

S

G

A

H
|
H

A

H

A

Y

L

F

K

R

E

E

N

F

Y

A

F

Y

L

A

D

D

D

S

G

G

A

-

Y

-

L

M

V

I

C

P

K

W

I

L

L

L

I

I

K

-

M

-

A

A

R

E

L

L

K

V

N

S

D

Q

S

S

N

G

G

R

K

S

L

L

S

A

D

D

L

L

I

V

S

-

D

-

L

-

D

-

S

-

P

-

C

-

E

-

S

-

K

-

E

E

Y

A

R

R

M

M

K

Q

I

-

K

K

F

F

N

P

D

C

F

S

K

G

E

E

Y

I

G

N

S

F

N

K

I

V

L

A

D

D

N

A

L

K

K

A

D

S

Y

V

V

A

K

R

S

V

I

M

N

E

G

H

W

Y

G

A

-

S

I

L

E

S

P

P

E

E

-

L

N

D

Y

Y

-

T

E

D

G

G

I

I

K

S

V

A

N

D

C

F

N

G

K

Q

E

-

N

-

G

-

D

-

G

-

W

W

-

R

F

F

L

N

L

L

R
|
R

L

S

S
|
S

L
x
N

H
x
T

E

E

A

P

V

L

M

L

P
x
R

L

L

N

N

I

V

E

E

S

T

active site: S97 (= S116), H98 (= H117), K107 (= K126), D237 (= D264), D239 (= D266), D241 (= D268), R242 (= R269), H324 (= H356)
binding 6-O-phosphono-alpha-D-glucopyranose: R280 (≠ V307), G302 (= G329), H303 (≠ Y330), E320 (= E352), H324 (= H356), R414 (= R462), S416 (= S464), N417 (≠ L465), T418 (≠ H466), R423 (≠ P471)
binding magnesium ion: S97 (= S116), D237 (= D264), D239 (= D266), D241 (= D268)

Sites not aligning to the query:

active site: 12

6nn2A Xanthomonas citri pgm apo-phospho (see paper)
30% identity, 84% coverage: 52:476/508 of query aligns to 35:428/448 of 6nn2A

query
sites
6nn2A

L

L

S

D

K

Q

L

G

K

P

I

V

S

V

I

L

G

G

M

H

D

D

S

V

R

R

I

L

T

A

S

S

E

P

S

A

I

L

K

Q

E

E

I

A

L

L

I

S

S

A

E

G

I

L

K

R

K

A

L

S

G

G

C

R

T

D

V

V

M

I

D

D

C

I

G

G

L

L

C

C

S

G

T

T

P

E

A

E

M

V

F

Y

M

F

S

Q

T

T

I

D

F

Y

E

-

N

-

Y

L

K

K

S

A

D

A

G

G

A

G

I

V

E

M

I

V

T

T

A

A

S
|
S

H
|
H

L

N

P

P

Y

M

Y

D

Y

Y

N

N

G

G

F

M

K
|
K

F

L

F

V

-

R

-

E

-

Q

-

A

-

R

-

P

-

I

-

S

T

S

N

D

K

T

G

G

G

L

F

F

D

A

K

I

E

R

D

D

I

T

N

V

E

A

L

A

L

D

D

T

I

A

A

A

Q

P

N

G

E

E

K

P

V

T

C

A

T

S

V

E

Q

Q

K

S

L

-

G

-

A

-

I

-

K

-

C

-

D

-

L

-

M

R

K

T

D

D

Y

K

S

T

N

A

F

Y

L

L

I

-

N

E

K

H

I

L

D

S

G

Y

I

V

D

D

D

-

K

-

E

R

H

S

R

T

L

L

K

K

P

P

L

L

E

-

N

-

F

-

K

K

I

L

V

V

D

N

A

A

G

G

N

N

G

G

A

G

G

A

G

G

F

L

F

I

V

V

E

D

K

L

V

L

L

A

N

P

Q

H

L

L

G

P

A

F

D

E

T

F

N

V

G

-

S

R

Q

V

F

F

I

H

E

E

P

P

D

D

G

G

T

N

F

F

P

P

N

N

H

G

I

I

P

P

N

N

P

P

E

L

K

L

K

P

E

E

A

N

M

R

D

D

S

A

L

T

K

A

K

K

A

A

V

V

L

K

E

D

N

N

K

G

A

A

D

D

I

F

G

G

I

I

I

A

F

W

D
|
D

T

G

D
|
D

V

F

D
|
D

R
|
R

A

C

A

F

I

F

V

F

D

D

S

H

Y

T

G

G

R

R

E

F

I

I

N

E

R

G

N

Y

L

Y

L

L

I

V

A

G

L

L

T

L

S

A

A

Q

I

A

V

I

L

L

E

A

E

K

H

Q

P

P

G

G

S

G

I

K

I

V

V

V

T

H

D

D

S

P

V

R

T

L

S

T

N

W

G

N

L

T

K

V

K

E

F

Q

I

V

E

E

K

E

R

A

G

G

I

I

H

P

H

V

R

L

F

C

K

K

R

S

G

G

Y

H

R

-

N

-

V

-

I

-

N

-

E

A

A

F

I

I

K

K

F

E

N

K

N

M

E

R

S

S

R

E

E

N

C

A

H

V

F

Y

A

G

I

G

E

E

T

M

S

S

G

A

H
|
H

A

H

A

Y

L

F

K

R

E

E

N

F

Y

A

F

Y

L

A

D

D

D

S

G

G

A

-

Y

-

L

M

V

I

C

P

K

W

I

L

L

L

I

I

K

-

M

-

A

A

R

E

L

L

K

V

N

S

D

Q

S

S

N

G

G

R

K

S

L

L

S

A

D

D

L

L

I

V

S

-

D

-

L

-

D

-

S

-

P

-

C

-

E

-

S

-

K

-

E

E

Y

A

R

R

M

M

K

Q

I

-

K

K

F

F

N

P

D

C

F

S

K

G

E

E

Y

I

G

N

S

F

N

K

I

V

L

A

D

D

N

A

L

K

K

A

D

S

Y

V

V

A

K

R

S

V

I

M

N

E

G

H

W

Y

G

A

-

S

I

L

E

S

P

P

E

E

-

L

N

D

Y

Y

-

T

E

D

G

G

I

I

K

S

V

A

N

D

C

F

N

G

K

Q

E

-

N

-

G

-

D

-

G

-

W

W

-

R

F

F

L

N

L

L

R

R

L

S

S

S

L

N

H

T

E

E

A

P

V

L

M

L

P

R

L

L

N

N

I

V

E

E

S

T

active site: S97 (= S116), H98 (= H117), K107 (= K126), D237 (= D264), D239 (= D266), D241 (= D268), R242 (= R269), H324 (= H356)
binding calcium ion: S97 (= S116), D237 (= D264), D239 (= D266), D241 (= D268)

Sites not aligning to the query:

active site: 12

6n1eA Crystal structure of x. Citri phosphoglucomutase in complex with 1- methyl-glucose 6-phosphate (see paper)
30% identity, 84% coverage: 52:476/508 of query aligns to 35:428/448 of 6n1eA

query
sites
6n1eA

L

L

S

D

K

Q

L

G

K

P

I

V

S

V

I

L

G

G

M

H

D

D

S

V

R

R

I

L

T

A

S

S

E

P

S

A

I

L

K

Q

E

E

I

A

L

L

I

S

S

A

E

G

I

L

K

R

K

A

L

S

G

G

C

R

T

D

V

V

M

I

D

D

C

I

G

G

L

L

C

C

S

G

T

T

P

E

A

E

M

V

F

Y

M

F

S

Q

T

T

I

D

F

Y

E

-

N

-

Y

L

K

K

S

A

D

A

G

G

A

G

I

V

E

M

I

V

T

T

A

A

S
|
S

H

H

L

N

P

P

Y

M

Y

D

Y

Y

N

N

G

G

F

M

K

K

F

L

F

V

-

R

-

E

-

Q

-

A

-

R

-

P

-

I

-

S

T

S

N

D

K

T

G

G

G

L

F

F

D

A

K

I

E

R

D

D

I

T

N

V

E

A

L

A

L

D

D

T

I

A

A

A

Q

P

N

G

E

E

K

P

V

T

C

A

T

S

V

E

Q

Q

K

S

L

-

G

-

A

-

I

-

K

-

C

-

D

-

L

-

M

R

K

T

D

D

Y

K

S

T

N

A

F

Y

L

L

I

-

N

E

K

H

I

L

D

S

G

Y

I

V

D

D

D

-

K

-

E

R

H

S

R

T

L

L

K

K

P

P

L

L

E

-

N

-

F

-

K

K

I

L

V

V

D

N

A

A

G

G

N

N

G

G

A

G

G

A

G

G

F

L

F

I

V

V

E

D

K

L

V

L

L

A

N

P

Q

H

L

L

G

P

A

F

D

E

T

F

N

V

G

-

S

R

Q

V

F

F

I

H

E

E

P

P

D

D

G

G

T

N

F

F

P

P

N

N

H

G

I

I

P

P

N

N

P

P

E

L

K

L

K

P

E

E

A

N

M

R

D

D

S

A

L

T

K

A

K

K

A

A

V

V

L

K

E

D

N

N

K

G

A

A

D

D

I

F

G

G

I

I

I

A

F

W

D
|
D

T

G

D
|
D

V

F

D
|
D

R

R

A

C

A

F

I

F

V

F

D

D

S

H

Y

T

G

G

R

R

E

F

I

I

N

E

R

G

N

Y

L

Y

L

L

I

V

A

G

L

L

T

L

S

A

A

Q

I

A

V

I

L

L

E

A

E

K

H

Q

P

P

G

G

S

G

I

K

I

V

V

V

T

H

D

D

S

P

V

R

T

L

S

T

N

W

G

N

L

T

K

V

K

E

F

Q

I

V

E

E

K

E

R

A

G

G

I

I

H

P

H

V

R

L

F

C

K

K

R

S

G
|
G

Y

H

R

-

N

-

V

-

I

-

N

-

E

A

A

F

I

I

K

K

F

E

N

K

N

M

E

R

S

S

R

E

E

N

C

A

H

V

F

Y

A

G

I

G

E
|
E

T

M

S

S

G

A

H
|
H

A

H

A

Y

L

F

K

R

E

E

N

F

Y

A

F

Y

L

A

D

D

D

S

G

G

A

-

Y

-

L

M

V

I

C

P

K

W

I

L

L

L

I

I

K

-

M

-

A

A

R

E

L

L

K

V

N

S

D

Q

S

S

N

G

G

R

K

S

L

L

S

A

D

D

L

L

I

V

S

-

D

-

L

-

D

-

S

-

P

-

C

-

E

-

S

-

K

-

E

E

Y

A

R

R

M

M

K

Q

I

-

K

K

F

F

N

P

D

C

F

S

K

G

E

E

Y

I

G

N

S

F

N

K

I

V

L

A

D

D

N

A

L

K

K

A

D

S

Y

V

V

A

K

R

S

V

I

M

N

E

G

H

W

Y

G

A

-

S

I

L

E

S

P

P

E

E

-

L

N

D

Y

Y

-

T

E

D

G

G

I

I

K

S

V

A

N

D

C

F

N

G

K

Q

E

-

N

-

G

-

D

-

G

-

W

W

-

R

F

F

L

N

L

L

R
|
R

L

S

S
|
S

L

N

H

T

E

E

A

P

V

L

M

L

P
x
R

L

L

N

N

I

V

E

E

S

T

binding magnesium ion: S97 (= S116), D237 (= D264), D239 (= D266), D241 (= D268)
binding 1-deoxy-7-O-phosphono-alpha-D-gluco-hept-2-ulopyranose: G302 (= G329), E320 (= E352), H324 (= H356), R414 (= R462), S416 (= S464), R423 (≠ P471)

6mnvA Crystal structure of x. Citri phosphoglucomutase in complex with ch2fg1p (see paper)
30% identity, 84% coverage: 52:476/508 of query aligns to 35:428/448 of 6mnvA

query
sites
6mnvA

L

L

S

D

K

Q

L

G

K

P

I

V

S

V

I

L

G

G

M

H

D

D

S

V

R

R

I

L

T

A

S

S

E

P

S

A

I

L

K

Q

E

E

I

A

L

L

I

S

S

A

E

G

I

L

K

R

K

A

L

S

G

G

C

R

T

D

V

V

M

I

D

D

C

I

G

G

L

L

C

C

S

G

T

T

P

E

A

E

M

V

F

Y

M

F

S

Q

T

T

I

D

F

Y

E

-

N

-

Y

L

K

K

S

A

D

A

G

G

A

G

I

V

E

M

I

V

T

T

A

A

S
|
S

H

H

L

N

P

P

Y

M

Y

D

Y

Y

N

N

G

G

F

M

K

K

F

L

F

V

-

R

-

E

-

Q

-

A

-

R

-

P

-

I

-

S

T

S

N

D

K

T

G

G

G

L

F

F

D

A

K

I

E

R

D

D

I

T

N

V

E

A

L

A

L

D

D

T

I

A

A

A

Q

P

N

G

E

E

K

P

V

T

C

A

T

S

V

E

Q

Q

K

S

L

-

G

-

A

-

I

-

K

-

C

-

D

-

L

-

M

R

K

T

D

D

Y

K

S

T

N

A

F

Y

L

L

I

-

N

E

K

H

I

L

D

S

G

Y

I

V

D

D

D

-

K

-

E

R

H

S

R

T

L

L

K

K

P

P

L

L

E

-

N

-

F

-

K

K

I

L

V

V

D

N

A

A

G

G

N

N

G

G

A

G

G

A

G

G

F

L

F

I

V

V

E

D

K

L

V

L

L

A

N

P

Q

H

L

L

G

P

A

F

D

E

T

F

N

V

G

-

S

R

Q

V

F

F

I

H

E

E

P

P

D

D

G

G

T

N

F

F

P

P

N

N

H

G

I

I

P

P

N

N

P

P

E

L

K

L

K

P

E

E

A

N

M

R

D

D

S

A

L

T

K

A

K

K

A

A

V

V

L

K

E

D

N

N

K

G

A

A

D

D

I

F

G

G

I

I

I

A

F

W

D
|
D

T

G

D
|
D

V

F

D
|
D

R

R

A

C

A

F

I

F

V

F

D

D

S

H

Y

T

G

G

R

R

E

F

I

I

N

E

R

G

N

Y

L

Y

L

L

I

V

A

G

L

L

T

L

S

A

A

Q

I

A

V

I

L

L

E

A

E

K

H

Q

P

P

G

G

S

G

I

K

I

V

V

V

T

H

D

D

S

P

V
x
R

T

L

S

T

N

W

G

N

L

T

K

V

K

E

F

Q

I

V

E

E

K

E

R

A

G

G

I

I

H

P

H

V

R

L

F

C

K

K

R

S

G
|
G

Y

H

R

-

N

-

V

-

I

-

N

-

E

A

A

F

I

I

K

K

F

E

N

K

N

M

E

R

S

S

R

E

E

N

C

A

H

V

F

Y

A

G

I

G

E
|
E

T

M

S
|
S

G

A

H
|
H

A

H

A

Y

L

F

K

R

E

E

N

F

Y

A

F

Y

L

A

D

D

D

S

G

G

A

-

Y

-

L

M

V

I

C

P

K

W

I

L

L

L

I

I

K

-

M

-

A

A

R

E

L

L

K

V

N

S

D

Q

S

S

N

G

G

R

K

S

L

L

S

A

D

D

L

L

I

V

S

-

D

-

L

-

D

-

S

-

P

-

C

-

E

-

S

-

K

-

E

E

Y

A

R

R

M

M

K

Q

I

-

K

K

F

F

N

P

D

C

F

S

K

G

E

E

Y

I

G

N

S

F

N

K

I

V

L

A

D

D

N

A

L

K

K

A

D

S

Y

V

V

A

K

R

S

V

I

M

N

E

G

H

W

Y

G

A

-

S

I

L

E

S

P

P

E

E

-

L

N

D

Y

Y

-

T

E

D

G

G

I

I

K

S

V

A

N

D

C

F

N

G

K

Q

E

-

N

-

G

-

D

-

G

-

W

W

-

R

F

F

L

N

L

L

R
|
R

L

S

S
|
S

L
x
N

H
x
T

E

E

A

P

V

L

M

L

P
x
R

L

L

N

N

I

V

E

E

S

T

binding 1-deoxy-1-fluoro-2-O-phosphono-alpha-D-gluco-hept-2-ulopyranose: R280 (≠ V307), G302 (= G329), E320 (= E352), S322 (= S354), H324 (= H356), R414 (= R462), S416 (= S464), N417 (≠ L465), T418 (≠ H466), R423 (≠ P471)
binding magnesium ion: S97 (= S116), D237 (= D264), D239 (= D266), D241 (= D268)

6mlhA Crystal structure of x. Citri phosphoglucomutase in complex with glucopyranosyl-1-methyl-phosphonic acid (see paper)
30% identity, 84% coverage: 52:476/508 of query aligns to 35:428/448 of 6mlhA

query
sites
6mlhA

L

L

S

D

K

Q

L

G

K

P

I

V

S

V

I

L

G

G

M

H

D

D

S

V

R

R

I

L

T

A

S

S

E

P

S

A

I

L

K

Q

E

E

I

A

L

L

I

S

S

A

E

G

I

L

K

R

K

A

L

S

G

G

C

R

T

D

V

V

M

I

D

D

C

I

G

G

L

L

C

C

S

G

T

T

P

E

A

E

M

V

F

Y

M

F

S

Q

T

T

I

D

F

Y

E

-

N

-

Y

L

K

K

S

A

D

A

G

G

A

G

I

V

E

M

I

V

T

T

A

A

S
|
S

H
|
H

L

N

P

P

Y

M

Y

D

Y

Y

N

N

G

G

F

M

K
|
K

F

L

F

V

-

R

-

E

-

Q

-

A

-

R

-

P

-

I

-

S

T

S

N

D

K

T

G

G

G

L

F

F

D

A

K

I

E

R

D

D

I

T

N

V

E

A

L

A

L

D

D

T

I

A

A

A

Q

P

N

G

E

E

K

P

V

T

C

A

T

S

V

E

Q

Q

K

S

L

-

G

-

A

-

I

-

K

-

C

-

D

-

L

-

M

R

K

T

D

D

Y

K

S

T

N

A

F

Y

L

L

I

-

N

E

K

H

I

L

D

S

G

Y

I

V

D

D

D

-

K

-

E

R

H

S

R

T

L

L

K

K

P

P

L

L

E

-

N

-

F

-

K

K

I

L

V

V

D

N

A

A

G

G

N

N

G

G

A

G

G

A

G

G

F

L

F

I

V

V

E

D

K

L

V

L

L

A

N

P

Q

H

L

L

G

P

A

F

D

E

T

F

N

V

G

-

S

R

Q

V

F

F

I

H

E

E

P

P

D

D

G

G

T

N

F

F

P

P

N

N

H

G

I

I

P

P

N

N

P

P

E

L

K

L

K

P

E

E

A

N

M

R

D

D

S

A

L

T

K

A

K

K

A

A

V

V

L

K

E

D

N

N

K

G

A

A

D

D

I

F

G

G

I

I

I

A

F

W

D
|
D

T

G

D
|
D

V

F

D
|
D

R
|
R

A

C

A

F

I

F

V

F

D

D

S

H

Y

T

G

G

R

R

E

F

I

I

N

E

R

G

N

Y

L

Y

L

L

I

V

A

G

L

L

T

L

S

A

A

Q

I

A

V

I

L

L

E

A

E

K

H

Q

P

P

G

G

S

G

I

K

I

V

V

V

T

H

D

D

S

P

V
x
R

T

L

S

T

N

W

G

N

L

T

K

V

K

E

F

Q

I

V

E

E

K

E

R

A

G

G

I

I

H

P

H

V

R

L

F

C

K

K

R

S

G
|
G

Y

H

R

-

N

-

V

-

I

-

N

-

E

A

A

F

I

I

K

K

F

E

N

K

N

M

E

R

S

S

R

E

E

N

C

A

H

V

F

Y

A

G

I

G

E
|
E

T

M

S

S

G

A

H
|
H

A

H

A

Y

L

F

K

R

E

E

N

F

Y

A

F

Y

L

A

D

D

D

S

G

G

A

-

Y

-

L

M

V

I

C

P

K

W

I

L

L

L

I

I

K

-

M

-

A

A

R

E

L

L

K

V

N

S

D

Q

S

S

N

G

G

R

K

S

L

L

S

A

D

D

L

L

I

V

S

-

D

-

L

-

D

-

S

-

P

-

C

-

E

-

S

-

K

-

E

E

Y

A

R

R

M

M

K

Q

I

-

K

K

F

F

N

P

D

C

F

S

K

G

E

E

Y

I

G

N

S

F

N

K

I

V

L

A

D

D

N

A

L

K

K

A

D

S

Y

V

V

A

K

R

S

V

I

M

N

E

G

H

W

Y

G

A

-

S

I

L

E

S

P

P

E

E

-

L

N

D

Y

Y

-

T

E

D

G

G

I

I

K

S

V

A

N

D

C

F

N

G

K

Q

E

-

N

-

G

-

D

-

G

-

W

W

-

R

F

F

L

N

L

L

R
|
R

L

S

S
|
S

L
x
N

H
x
T

E

E

A

P

V

L

M

L

P
x
R

L

L

N

N

I

V

E

E

S

T

active site: S97 (= S116), H98 (= H117), K107 (= K126), D237 (= D264), D239 (= D266), D241 (= D268), R242 (= R269), H324 (= H356)
binding (1S)-1,5-anhydro-1-(phosphonomethyl)-D-glucitol: R280 (≠ V307), G302 (= G329), E320 (= E352), H324 (= H356), R414 (= R462), S416 (= S464), N417 (≠ L465), T418 (≠ H466), R423 (≠ P471)
binding magnesium ion: S97 (= S116), D237 (= D264), D239 (= D266), D241 (= D268)

Sites not aligning to the query:

active site: 12

Query Sequence

>WP_026142645.1 NCBI__GCF_000816635.1:WP_026142645.1
MDSKWENVKNSTDIRGVVIKNSERDVNLIDSMISSIAKAFVLLLSRKKNRDLSKLKISIG
MDSRITSESIKEILISEIKKLGCTVMDCGLCSTPAMFMSTIFENYKSDGAIEITASHLPY
YYNGFKFFTNKGGFDKEDINELLDIAQNEKVCTVQKLGAIIKCDLMKDYSNFLINKIIDG
IDDKEHRLKPLENFKIVVDAGNGAGGFFVEKVLNQLGADTNGSQFIEPDGTFPNHIPNPE
KKEAMDSLKKAVLENKADIGIIFDTDVDRAAIVDSYGREINRNLLIALTSAIVLEEHPGS
IIVTDSVTSNGLKKFIEKRGGIHHRFKRGYRNVINEAIKFNNESRECHFAIETSGHAALK
ENYFLDDGAYLVCKILIKMARLKNDSNGKLSDLISDLDSPCESKEYRMKIKFNDFKEYGS
NILDNLKDYVKSINGWGIEPENYEGIKVNCNKENGDGWFLLRLSLHEAVMPLNIESDSQG
GVEFIVPKLIKYLMKYDKLDLSSIIDRQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory