SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_026595805.1 NCBI__GCF_000385335.1:WP_026595805.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q5F8J4 Homoserine dehydrogenase; HDH; HSD; NAD(+)-dependent homoserine dehydrogenase; NAD(+)-dependent HSD; NgHSD; EC 1.1.1.3 from Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) (see paper)
43% identity, 95% coverage: 23:435/437 of query aligns to 22:431/435 of Q5F8J4

query
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Q5F8J4

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L45 (≠ K46) mutation to R: Shows a marked increase in the catalytic efficiency with NADP(+).
LS 45:46 (≠ KA 46:47) mutation to RD: Does not impair the catalytic activity with NAD(+). Slightly increases the activity, but slightly decreases the affinity for NADP(+).; mutation to RR: Causes a shift in coenzyme preference from NAD(+) to NADP(+) by a factor of 974. Shows a slight decrease in the catalytic efficiency with NAD(+) and a 4.5-fold increase in catalytic efficiency with NADP(+).

6dzsA Mycobacterial homoserine dehydrogenase thra in complex with NADP
37% identity, 98% coverage: 7:435/437 of query aligns to 7:429/431 of 6dzsA

query
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binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G11), L12 (= L12), G13 (= G13), N14 (≠ T14), V15 (= V15), V45 (≠ A45), R46 (≠ K46), R47 (≠ A47), R52 (= R52), I63 (≠ P65), L78 (= L80), M79 (≠ I81), P84 (≠ A87), A102 (= A105), K104 (= K107), G306 (= G304), T310 (= T308)

4pg7A Crystal structure of s. Aureus homoserine dehydrogenase at ph7.5 (see paper)
38% identity, 73% coverage: 2:320/437 of query aligns to 2:307/402 of 4pg7A

query
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4pg7A

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active site: D190 (= D203), K194 (= K207)
binding lysine: R215 (≠ E228), G219 (≠ T232)

Sites not aligning to the query:

binding lysine: 394, 395

6a0sA Homoserine dehydrogenase from thermus thermophilus hb8 complexed with hse and NADPH (see paper)
38% identity, 78% coverage: 2:344/437 of query aligns to 1:323/331 of 6a0sA

query
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6a0sA

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V

V

V

E

E

L
x
A

I
x
M

G

G

E

V

E

E

G

A

A

P

A

L

R

R

A

L

A

-

V

V

E

L

T

P

A

A

I

L

A

E

A

A

G

G

K

I

S

P

V

L

V

I

T

T

A
|
A

N

N

K
|
K

A

A

L

L

A

A

K

E

H

A

G

W

M

E

A

S

L

L

A

R

R

P

A

F

A

A

E

E

K

G

G

-

V

-

A

L

L

I

A

Y

F

H

E

E

A

A

S

S

V

V

A

M

G

A

G

G

I

T

P

P

V

A

I

L

K

S

T

F

L

L

R

-

E

E

G

T

F

L

A

R

G

G

N

S

A

E

I

L

E

L

R

E

I

L

Y

H

G

G

I

I

L

L

N
|
N

G
|
G

T
|
T

C

T

N

L

Y

Y

I

I

L

L

S

Q

R

E

M

M

E

E

T

K

E

G

K

R

L

-

S

T

F

Y

A

A

A

E

C

A

L

L

R

L

E

E

A

A

Q

Q

E

R

L

L

G
|
G

Y
|
Y

A

A

E
|
E

A

A

D

D

P

P

T

T

F

L

D
|
D

I

V

G

E

G

G

F

I

D
|
D

T

A

A

A

H

H

K
|
K

L

L

A

T

I

L

L

L

T

A

A

R

L

L

A

L

F

V

G

D

T

P

T

G

I

F

D

P

P

F

D

A

A

E

I

V

A

E

I

A

E

Q

G

G

I

I

E

A

T

R

I

L

T

T

L

P

A

E

D

V

L

L

E

Q

A

K

A

A

D

E

E

A

L

R

G

G

Y

E

R

R

V

V

K

R

L

L

L

-

G

-

V

V

A

A

Q

S

K

L

T

F

L

G

S

E

G

G

I

G

E

R

Q

W

R

R

-

A

V

V

H

A

P

P

T

R

M

R

V

L

P

P

K

Q

S

D

S

H

A

P

I

L

A

A

Q

R

I

A

M

R

G

G

V

-

I

-

N

N

A

A

V

L

S

W

I

V

N

R

A

A

D

R

A

P

V

L

H

G

E

E

L

A

T

F

L

V

A

T

G

G

P

P

G

G

A
|
A

G
|
G

G

G

E

G

A

A

T
|
T

A

A

S

S

A

G

V

L

V

F

A

A

D

D

L

L

A

L

D

R

L

F

A

L

R

S

G

G

S

-

H

-

I

-

A

A

P

P

F

G

G

H

L

L

P

P

V

-

A

-

D

-

L

-

A

A

P

P

L

R

Q

A

R

R

A

P

P

P

L

L

Q

E

active site: D191 (= D203), K195 (= K207)
binding l-homoserine: K99 (= K107), N150 (= N161), G151 (= G162), T152 (= T163), Y178 (= Y190), E180 (= E192), D186 (= D198), K195 (= K207)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (≠ L12), G12 (= G13), T13 (= T14), V14 (= V15), L42 (≠ S44), V43 (≠ A45), R44 (≠ K46), D45 (≠ A47), K48 (= K50), R50 (= R52), A73 (≠ L80), M74 (≠ I81), A97 (= A105), K99 (= K107), G177 (= G189), E180 (= E192), A289 (= A303), G290 (= G304), T294 (= T308)

2ejwA Homoserine dehydrogenase from thermus thermophilus hb8
38% identity, 78% coverage: 2:344/437 of query aligns to 1:323/331 of 2ejwA

query
sites
2ejwA

M

M

R

E

H

A

L

L

R

K

V

I

G

A

I

L

A

L

G

G

L

G

G

G

T

T

V

V

G

G

T

S

A

A

V

F

V

Y

R

N

L

L

L

V

D

L

R

E

H

R

A

A

Q

E

A

E

L

L

A

S

A

A

R

-

T

F

G

G

Q

V

R

V

I

P

E

R

V

F

T

L

A

G

V

V

S

L

A

V

K

R

A

D

R

P

D

R

K

K

D

P

R

R

G

A

I

I

-

P

-

Q

D

E

L

L

S

L

R

R

F

A

A

E

W

P

F

F

D

D

D

-

P

-

V

-

A

-

L

-

A

-

S

L

P

L

D

E

I

A

D

D

V

L

F

V

V

V

E

E

L

A

I

M

G

G

E

V

E

E

G

A

A

P

A

L

R

R

A

L

A

-

V

V

E

L

T

P

A

A

I

L

A

E

A

A

G

G

K

I

S

P

V

L

V

I

T

T

A

A

N

N

K

K

A

A

L

L

A

A

K

E

H

A

G

W

M

E

A

S

L

L

A

R

R

P

A

F

A

A

E

E

K

G

G

-

V

-

A

L

L

I

A

Y

F

H

E
|
E

A

A

S

S

V
|
V

A
x
M

G
x
A

G

G

I
x
T

P

P

V

A

I

L

K

S

T

F

L

L

R

-

E

E

G

T

F

L

A

R

G

G

N

S

A

E

I

L

E

L

R

E

I

L

Y

H

G

G

I

I

L

L

N

N

G

G

T

T

C

T

N

L

Y

Y

I

I

L

L

S

Q

R

E

M

M

E

E

T

K

E

G

K

R

L

-

S

T

F

Y

A

A

A

E

C

A

L

L

R

L

E

E

A

A

Q

Q

E

R

L

L

G

G

Y

Y

A

A

E

E

A

A

D

D

P

P

T

T

F

L

D

D

I

V

G

E

G

G

F

I

D
|
D

T

A

A

A

H

H

K
|
K

L

L

A

T

I

L

L

L

T

A

A

R

L

L

A

L

F

V

G

D

T

P

T

G

I

F

D

P

P

F

D

A

A

E

I

V

A

E

I

A

E

Q

G

G

I

I

E

A

T

R

I

L

T

T

L

P

A

E

D

V

L

L

E

Q

A

K

A

A

D

E

E

A

L

R

G

G

Y

E

R

R

V

V

K

R

L

L

L

-

G

-

V

V

A

A

Q

S

K

L

T

F

L

G

S

E

G

G

I

G

E

R

Q

W

R

R

-

A

V

V

H

A

P

P

T

R

M

R

V

L

P

P

K

Q

S

D

S

H

A

P

I

L

A

A

Q

R

I

A

M

R

G

G

V

-

I

-

N

N

A

A

V

L

S

W

I

V

N

R

A

A

D

R

A

P

V

L

H

G

E

E

L

A

T

F

L

V

A

T

G

G

P

P

G

G

A

A

G

G

E

G

A

A

T

T

A

A

S

S

A

G

V

L

V

F

A

A

D

D

L

L

A

L

D

R

L

F

A

L

R

S

G

G

S

-

H

-

I

-

A

A

P

P

F

G

G

H

L

L

P

P

V

-

A

-

D

-

L

-

A

A

P

P

L

R

Q

A

R

R

A

P

P

P

L

L

Q

E

active site: D191 (= D203), K195 (= K207)
binding magnesium ion: E121 (= E131), V124 (= V134), M125 (≠ A135), A126 (≠ G136), T128 (≠ I138)

6a0tB Homoserine dehydrogenase k99a mutant from thermus thermophilus hb8 complexed with hse and NADP+ (see paper)
38% identity, 78% coverage: 2:344/437 of query aligns to 1:323/332 of 6a0tB

query
sites
6a0tB

M

M

R

E

H

A

L

L

R

K

V

I

G

A

I

L

A

L

G
|
G

L
x
G

G
|
G

T
|
T

V
|
V

G

G

T

S

A

A

V

F

V

Y

R

N

L

L

L

V

D

L

R

E

H

R

A

A

Q

E

A

E

L

L

A

S

A

A

R

-

T

F

G

G

Q

V

R

V

I

P

E

R

V

F

T

L

A

G

V

V

S
x
L

A
x
V

K
x
R

A
x
D

R

P

D

R

K
|
K

D

P

R
|
R

G

A

I

I

-

P

-

Q

D

E

L

L

S

L

R

R

F

A

A

E

W

P

F

F

D

D

D

-

P

-

V

-

A

-

L

-

A

-

S

L

P

L

D

E

I

A

D

D

V

L

F

V

V

V

E

E

L
x
A

I
x
M

G
|
G

G

G

E

V

E

E

G

A

A

P

A

L

R

R

A

L

A

-

V

V

E

L

T

P

A

A

I

L

A

E

A

A

G

G

K

I

S

P

V

L

V

I

T

T

A
|
A

N
|
N

K

K

A

A

L

L

A

A

K

E

H

A

G

W

M

E

A

S

L

L

A

R

R

P

A

F

A

A

E

E

K

G

G

-

V

-

A

L

L

I

A

Y

F

H

E

E

A

A

S

S

V

V

A

M

G

A

G

G

I

T

P

P

V

A

I

L

K

S

T

F

L

L

R

-

E

E

G

T

F

L

A

R

G

G

N

S

A

E

I

L

E

L

R

E

I

L

Y

H

G

G

I

I

L

L

N
|
N

G
|
G

T
|
T

C

T

N

L

Y

Y

I

I

L

L

S

Q

R

E

M

M

E

E

T

K

E

G

K

R

L

-

S

T

F

Y

A

A

A

E

C

A

L

L

R

L

E

E

A

A

Q

Q

E

R

L

L

G
|
G

Y
|
Y

A

A

E
|
E

A

A

D

D

P

P

T

T

F

L

D
|
D

I

V

G

E

G

G

F

I

D
|
D

T

A

A

A

H

H

K
|
K

L

L

A

T

I

L

L

L

T

A

A

R

L

L

A

L

F

V

G

D

T

P

T

G

I

F

D

P

P

F

D

A

A

E

I

V

A

E

I

A

E

Q

G

G

I

I

E

A

T

R

I

L

T

T

L

P

A

E

D

V

L

L

E

Q

A

K

A

A

D

E

E

A

L

R

G

G

Y

E

R

R

V

V

K

R

L

L

L

-

G

-

V

V

A

A

Q

S

K

L

T

F

L

G

S

E

G

G

I

G

E

R

Q

W

R

R

-

A

V

V

H

A

P

P

T

R

M

R

V

L

P

P

K

Q

S

D

S

H

A

P

I

L

A

A

Q

R

I

A

M

R

G

G

V

-

I

-

N

N

A

A

V

L

S

W

I

V

N

R

A

A

D

R

A

P

V

L

H

G

E

E

L

A

T

F

L

V

A

T

G

G

P

P

G

G

A
|
A

G
|
G

G

G

E

G

A

A

T
|
T

A

A

S

S

A

G

V

L

V

F

A

A

D

D

L

L

A

L

D

R

L

F

A

L

R

S

G

G

S

-

H

-

I

-

A

A

P

P

F

G

G

H

L

L

P

P

V

-

A

-

D

-

L

-

A

A

P

P

L

R

Q

A

R

R

A

P

P

P

L

L

Q

E

active site: D191 (= D203), K195 (= K207)
binding l-homoserine: N150 (= N161), G151 (= G162), T152 (= T163), Y178 (= Y190), E180 (= E192), D186 (= D198), K195 (= K207)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G11), G11 (≠ L12), G12 (= G13), T13 (= T14), V14 (= V15), L42 (≠ S44), V43 (≠ A45), R44 (≠ K46), D45 (≠ A47), K48 (= K50), R50 (= R52), A73 (≠ L80), M74 (≠ I81), G75 (= G82), A97 (= A105), N98 (= N106), G177 (= G189), E180 (= E192), A289 (= A303), G290 (= G304), T294 (= T308)

F9VNG5 Homoserine dehydrogenase; HDH; HSD; StHSD; EC 1.1.1.3 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 3 papers)
35% identity, 57% coverage: 71:321/437 of query aligns to 65:301/304 of F9VNG5

query
sites
F9VNG5

S

K

P

P

D

D

I

I

D

I

V

V

F

D

V

V

E
x
S

L

S

I

A

G

N

G

Y

E

N

G

G

A

E

A

P

R

S

A

L

A

S

V

L

-

Y

E

K

T

E

A

A

I

I

A

K

A

D

G

G

K

V

S

N

V

I

T

T

A
x
T

N

N

K
|
K

A

A

L

P

L

L

A

A

K

L

H

A

G

F

M

N

A

E

L

I

A

F

R

S

A

L

A

A

E

R

E

S

K

K

G

G

V

V

A

K

L

I

A

G

F

F

E

Q

A

G

S

T

V

V

A

M

G

S

G

G

I

T

P

P

V

S

I

I

K

N

T

L

L

Y

R

R

E

V

G

-

F

L

A

P

G

G

N

S

A

R

I

V

E

I

R

K

I

I

Y

R

G

G

I

I

L

L

N

N

G

G

T

T

C

T

N

N

Y

F

I

I

L

L

S

T

R

L

M

M

E

-

T

N

E

K

K

G

L

V

S

S

F

F

A

E

C

A

L

L

R

K

E

E

A

A

Q

Q

E

R

L

R

G
|
G

Y

Y

A

A

E
|
E

A

E

D

D

P

P

T

T

F

L

D

D

I

I

G

N

G

G

F

F

D

D

T

A

A

A

H

A

K

K

L

I

A

T

I

I

L

L

T

A

A

N

L

F

A

M

F

I

G

G

T

N

T

S

I

V

D

T

P

I

D

K

A

D

I

V

A

K

I

F

E

E

G

G

I

I

E

N

T

R

I

-

T

-

L

-

A

-

D

D

L

L

E

P

A

K

A

N

D

E

E

-

L

-

G

-

Y

-

R

K

V

I

K

K

L

L

L

I

G

A

V

Y

A

A

Q

D

K

E

T

K

L

-

S

-

G

-

I

-

E

E

Q

V

R

W

V

V

H

K

P

P

T

L

M

P

V

I

P

S

K

Q

S

D

A

P

I

L

A

Y

Q

N

I

V

M

D

G

G

V

V

I

E

N

N

A

A

V

L

S

E

I

I

N

T

A

T

D

D

A

-

V

I

H

Q

E

S

L

I

T

L

L

I

A

R

G

G

P

P

G

G

A

A

G
|
G

G

P

E

V

A

N

T

A

A

A

S

Y

A

G

V

A

V

L

A

S

D

D

L

L

A

I

D

L

L

L

A

K

R

R

S73 (≠ E79) binding NADP(+)
T100 (≠ A105) binding NADP(+)
K102 (= K107) binding NADP(+)
G182 (= G189) binding NADP(+)
E185 (= E192) binding NADP(+)
G284 (= G304) binding NADP(+)

Sites not aligning to the query:

8 binding NADP(+)
10 binding NADP(+)
11 binding NADP(+)
38 binding NADP(+)
39 binding NADP(+)
303 modified: Interchain

7f4cA The crystal structure of the immature holo-enzyme of homoserine dehydrogenase complexed with NADP and 1,4-butandiol from the hyperthermophilic archaeon sulfurisphaera tokodaii. (see paper)
35% identity, 57% coverage: 71:321/437 of query aligns to 65:297/300 of 7f4cA

query
sites
7f4cA

S

K

P

P

D

D

I

I

D

I

V

V

F

D

V
|
V

E
x
S

L
x
S

I

A

G

N

G

Y

E

N

G

G

A

E

A

P

R

S

A

L

A

S

V

L

-

Y

E

K

T

E

A

A

I

I

A

K

A

D

G

G

K

V

S

N

V

I

T

T

A
x
T

N

N

K
|
K

A

A

L

P

L

L

A

A

K

L

H

A

G

F

M

N

A

E

L

I

A

F

R

S

A

L

A

A

E

R

E

S

K

K

G

G

V

V

A

K

L

I

A

G

F

F

E

Q

A
x
G

S

T

V

V

A

M

G
x
S

G

G

I

T

P

P

V

S

I

I

K

N

T

L

L

Y

R

R

E

V

G

-

F

L

A

P

G

G

N

S

A

R

I

V

E

I

R

K

I

I

Y

R

G

G

I

I

L

L

N

N

G

G

T

T

C

T

N

N

Y

F

I

I

L

L

S

T

R

L

M

M

E

-

T

N

E

K

K

G

L

V

S

S

F

F

A

E

C

A

L

L

R

K

E

E

A

A

Q

Q

E

R

L

R

G

G

Y

Y

A

A

E
|
E

A

E

D

D

P

P

T

T

F

L

D

D

I

I

G

N

G

G

F

F

D

D

T

A

A

A

H

A

K

K

L

I

A

T

I

I

L

L

T

A

A

N

L

F

A

M

F

I

G

G

T

N

T

S

I

V

D

T

P

I

D

K

A

D

I

V

A

K

I

F

E

E

G

G

I

I

E

N

T

R

I

-

T

-

L

-

A

-

D

D

L

L

E

-

A

-

D

-

E

-

L

-

G

-

Y

-

R

K

V

I

K

K

L

L

L

I

G

A

V

Y

A

A

Q

D

K

E

T

K

L

-

S

-

G

-

I

-

E

E

Q

V

R

W

V

V

H

K

P

P

T

L

M

P

V

I

P

S

K

Q

S

D

A

P

I

L

A

Y

Q

N

I

V

M

D

G

G

V

V

I

E

N

N

A

A

V

L

S

E

I

I

N

T

A

T

D

D

A

-

V

I

H

Q

E

S

L

I

T

L

L

I

A

R

G

G

P

P

G

G

A

A

G
|
G

G

P

E

V

A

N

T
x
A

A

A

S

Y

A

G

V

A

V

L

A

S

D

D

L

L

A

I

D

L

L

L

A

K

R

R

binding nadp nicotinamide-adenine-dinucleotide phosphate: V72 (= V78), S73 (≠ E79), S74 (≠ L80), T100 (≠ A105), K102 (= K107), G127 (≠ A132), S131 (≠ G136), E185 (= E192), G280 (= G304), A284 (≠ T308)

Sites not aligning to the query:

binding nadp nicotinamide-adenine-dinucleotide phosphate: 6, 7, 8, 9, 10, 11, 37, 38, 39

5x9dA Crystal structure of homoserine dehydrogenase in complex with l- cysteine and NAD (see paper)
34% identity, 57% coverage: 71:321/437 of query aligns to 65:299/302 of 5x9dA

query
sites
5x9dA

S

K

P

P

D

D

I

I

D

I

V

V

F

D

V
|
V

E
x
S

L
x
S

I

A

G

N

G

Y

E

N

G

G

A

E

A
x
P

R

S

A

L

A

S

V

L

-

Y

E

K

T

E

A

A

I

I

A

K

A

D

G

G

K

V

S

N

V

I

T

T

A
x
T

N
|
N

K
|
K

A

A

L

P

L

L

A

A

K

L

H

A

G

F

M

N

A

E

L

I

A

F

R

S

A

L

A

A

E

R

E

S

K

K

G

G

V

V

A

K

L

I

A

G

F

F

E

Q

A
x
G

S

T

V

V

A

M

G
x
S

G

G

I

T

P

P

V

S

I

I

K

N

T

L

L

Y

R

R

E

V

G

-

F

L

A

P

G

G

N

S

A

R

I

V

E

I

R

K

I

I

Y

R

G

G

I

I

L

L

N
|
N

G
|
G

T
|
T

C

T

N

N

Y

F

I

I

L

L

S

T

R

L

M

M

E

-

T

N

E

K

K

G

L

V

S

S

F

F

A

E

C

A

L

L

R

K

E

E

A

A

Q

Q

E

R

L

R

G

G

Y
|
Y

A
|
A

E
|
E

A

E

D

D

P

P

T

T

F

L

D
|
D

I

I

G

N

G

G

F

F

D
|
D

T

A

A

A

H

A

K
|
K

L

I

A

T

I

I

L

L

T

A

A

N

L

F

A

M

F

I

G

G

T

N

T

S

I

V

D

T

P

I

D

K

A

D

I

V

A

K

I

F

E

E

G

G

I

I

E

N

T

R

I

-

T

-

L

-

A

-

D

-

L

-

E

-

A

-

D

D

E

P

L

K

G

N

Y

E

R

K

V

I

K

K

L

L

L

I

G

A

V

Y

A

A

Q

D

K

E

T

K

L

-

S

-

G

-

I

-

E

E

Q

V

R

W

V

V

H

K

P

P

T

L

M

P

V

I

P

S

K

Q

S

-

S

D

A

P

I

L

A

Y

Q

N

I

V

M

D

G

G

V

V

I

E

N

N

A

A

V

L

S

E

I

I

N

T

A

T

D

D

A

-

V

I

H

Q

E

S

L

I

T

L

L

I

A

R

G

G

P

P

G

G

A
|
A

G
|
G

G

P

E

V

A

N

T
x
A

A

A

S

Y

A

G

V

A

V

L

A

S

D

D

L

L

A

I

D

L

L

L

A

K

R

R

active site: D196 (= D203), K200 (= K207)
binding (2R)-3-[[(4S)-3-aminocarbonyl-1-[(2R,3R,4S,5R)-5-[[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxymethyl]-3,4-bis(oxidanyl)oxolan-2-yl]-4H-pyridin-4-yl]sulfanyl]-2-azanyl-propanoic acid: V72 (= V78), S73 (≠ E79), S74 (≠ L80), P82 (≠ A88), T100 (≠ A105), N101 (= N106), K102 (= K107), G127 (≠ A132), S131 (≠ G136), N155 (= N161), G156 (= G162), T157 (= T163), Y183 (= Y190), A184 (= A191), E185 (= E192), D191 (= D198), D196 (= D203), K200 (= K207), A281 (= A303), G282 (= G304), A286 (≠ T308)

Sites not aligning to the query:

binding (2R)-3-[[(4S)-3-aminocarbonyl-1-[(2R,3R,4S,5R)-5-[[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxymethyl]-3,4-bis(oxidanyl)oxolan-2-yl]-4H-pyridin-4-yl]sulfanyl]-2-azanyl-propanoic acid: 6, 7, 8, 9, 10, 11, 37, 38

4xb1A Hyperthermophilic archaeal homoserine dehydrogenase in complex with NADPH (see paper)
28% identity, 72% coverage: 4:316/437 of query aligns to 2:308/319 of 4xb1A

query
sites
4xb1A

H

K

L

V

R

N

V

I

G

S

I

I

A
x
F

G

G

L
x
F

G
|
G

T
|
T

V
|
V

G

G

T

R

A

A

V

L

V

A

R

E

L

I

D

A

R

E

H

K

A

S

Q

R

-

I

-

F

-

G

-

V

-

E

-

L

-

N

-

V

-

I

A

S

L

I

A

T

A
x
D

R
|
R

T

S

G

G

Q

T

R

I

I

W

-

G

E

D

V

F

T

D

A

L

V

L

S

E

A

A

K

K

A

E

R

V

D
x
K

K

E

D

S

R

T

G

G

I

-

D

K

L

L

S

S

R

N

F

I

A

G

W

D

F

Y

D

E

-

V

-

Y

-

N

-

F

D

S

P

P

V

Q

A

E

L

L

A

V

A

E

S

E

P

V

D

K

I

P

D

N

V

I

F

L

V

V

E

D

L
x
V

I
x
S

G
x
S

G

W

E

D

E

E

G

-

A

-

A

A

R

H

A

E

A

M

V

Y

E

K

T

V

A

A

I

L

A

G

A

E

G

G

K

I

S

S

V

V

T

T

A
x
S

N

N

K
|
K

A

P

L

P

L

I

A

A

K

N

H

Y

G

Y

M

D

A

E

L

L

A

M

R

N

A

L

A

A

E

K

E

E

K

N

G

N

V

A

A

G

L

I

A

F

F

F

E

E

A
x
S

S

T

V

V

A

M

G

A

G

G

I

T

P

P

V

I

I

I

K

G

T

V

L

L

R

R

E

E

G

N

F

L

A

L

G

G

N

E

A

N

I

I

E

K

R

R

I

I

Y

D

G

A

I

V

L

V

N

N

G

A

T

S

C

T

N

T

Y

F

I

I

L

L

S

T

R

K

M

M

E

-

T

S

E

E

K

G

L

K

S

T

F

L

A

D

C

A

L

I

R

E

E

E

A

A

Q

K

E

S

L

L

G

G

Y

I

A

L

E

E

A

E

D

D

P

P

T

S

F

K

D

D

I

I

G

D

G

G

F

I

D
|
D

T

A

A

Y

H

Y

K
|
K

L

A

A

K

I

I

L

L

T

H

A

W

L

V

A

S

F

Y

G

G

T

E

T

P

I

-

D

-

P

P

D

E

A

-

I

-

A

-

I

-

E

-

G

-

I

-

E

E

T

E

I

E

T

R

L

L

A

G

D

I

L

R

E

E

A

V

A

R

D

D

E

A

L

R

G

N

Y

V

R

R

V

L

K

-

L

-

G

-

V

V

A

A

Q

Q

K

V

T

S

L

K

S

G

G

K

I

I

E

S

Q

-

R

-

V

V

H

K

P

P

T

R

M

K

V

L

P

S

K

S

S

D

S

N

A

P

I

L

A

L

Q

-

I

V

M

E

G

G

V

V

I

Q

N

N

A

A

V

A

S

V

I

I

N

R

A

T

D

N

A

N

V

L

H

G

E

E

L

V

T

I

L

L

A

K

G

G

P

P

G

G

A
x
G

G

G

E

R

A

V

T
|
T

A

A

S

S

A

G

V

V

V

F

A

T

D

D

L

I

active site: D211 (= D203), K215 (= K207)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F8 (≠ A10), F10 (≠ L12), G11 (= G13), T12 (= T14), V13 (= V15), D39 (≠ A32), R40 (= R33), K57 (≠ D49), V91 (≠ L80), S92 (≠ I81), S93 (≠ G82), S114 (≠ A105), K116 (= K107), S141 (≠ A132), G295 (≠ A303), T300 (= T308)

O58802 Homoserine dehydrogenase; HDH; HSD; HseDH; EC 1.1.1.3 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see paper)
28% identity, 72% coverage: 4:316/437 of query aligns to 2:308/319 of O58802

query
sites
O58802

H

K

L

V

R

N

V

I

G

S

I

I

A

F

G

G

L
x
F

G

G

T
|
T

V
|
V

G

G

T

R

A

A

V

L

V

A

R

E

L

I

D

A

R

E

H

K

A

S

Q

R

-

I

-

F

-

G

-

V

-

E

-

L

-

N

-

V

-

I

A

S

L

I

A

T

A

D

R
|
R

T

S

G

G

Q

T

R

I

I

W

-

G

E

D

V

F

T

D

A

L

V

L

S

E

A

A

K

K

A

E

R

V

D
x
K

K

E

D

S

R

T

G

G

I

-

D

K

L

L

S

S

R

N

F

I

A

G

W

D

F

Y

D

E

-

V

-

Y

-

N

-

F

D

S

P

P

V

Q

A

E

L

L

A

V

A

E

S

E

P

V

D

K

I

P

D

N

V

I

F

L

V

V

E

D

L

V

I
x
S

G
x
S

G

W

E

D

E

E

G

-

A

-

A

A

R

H

A

E

A

M

V

Y

E

K

T

V

A

A

I

L

A

G

A

E

G

G

K

I

S

S

V

V

T

T

A
x
S

N

N

K
|
K

A

P

L

P

L

I

A

A

K

N

H

Y

G

Y

M

D

A

E

L

L

A

M

R

N

A

L

A

A

E

K

E

E

K

N

G

N

V

A

A

G

L

I

A

F

F

F

E
|
E

A

S

S

T

V
|
V

A

M

G
x
A

G

G

I
x
T

P

P

V

I

I

I

K

G

T

V

L

L

R

R

E

E

G

N

F

L

A

L

G

G

N

E

A

N

I

I

E

K

R

R

I

I

Y

D

G

A

I

V

L

V

N

N

G

A

T

S

C

T

N

T

Y

F

I

I

L

L

S

T

R

K

M

M

E

-

T

S

E

E

K

G

L

K

S

T

F

L

A

D

C

A

L

I

R

E

E

E

A

A

Q

K

E

S

L

L

G

G

Y

I

A

L

E

E

A

E

D

D

P

P

T

S

F

K

D

D

I

I

G

D

G

G

F

I

D

D

T

A

A

Y

H

Y

K

K

L

A

A

K

I

I

L

L

T

H

A

W

L

V

A

S

F

Y

G

G

T

E

T

P

I

-

D

-

P

P

D

E

A

-

I

-

A

-

I

-

E

-

G

-

I

-

E

E

T

E

I

E

T

R

L

L

A

G

D

I

L

R

E

E

A

V

A

R

D

D

E

A

L

R

G

N

Y

V

R

R

V

L

K

-

L

-

G

-

V

V

A

A

Q

Q

K

V

T

S

L

K

S

G

G

K

I

I

E

S

Q

-

R

-

V

V

H

K

P

P

T

R

M

K

V

L

P

S

K

S

S

D

S

N

A

P

I

L

A

L

Q

-

I

V

M

E

G

G

V

V

I

Q

N

N

A

A

V

A

S

V

I

I

N

R

A

T

D

N

A

N

V

L

H

G

E

E

L

V

T

I

L

L

A

K

G

G

P

P

G

G

A

G

G
|
G

G

G

E

R

A

V

T

T

A

A

S

S

A

G

V

V

V

F

A

T

D

D

L

I

F10 (≠ L12) binding NADPH
T12 (= T14) binding NADPH
V13 (= V15) binding NADPH
R40 (= R33) binding NADPH; mutation to A: Increases activity with NADP.
K57 (≠ D49) binding NADPH; mutation to A: Increases activity with NADP.
S92 (≠ I81) binding NADPH
S93 (≠ G82) binding NADPH
S114 (≠ A105) binding NADPH
K116 (= K107) binding NADPH
E140 (= E131) binding Na(+)
V143 (= V134) binding Na(+)
A145 (≠ G136) binding Na(+)
T147 (≠ I138) binding Na(+)
G296 (= G304) binding NADPH

4xb2A Hyperthermophilic archaeal homoserine dehydrogenase mutant in complex with NADPH (see paper)
28% identity, 72% coverage: 4:316/437 of query aligns to 2:308/319 of 4xb2A

query
sites
4xb2A

H

K

L

V

R

N

V

I

G

S

I

I

A
x
F

G

G

L
x
F

G
|
G

T
|
T

V
|
V

G

G

T

R

A

A

V

L

V

A

R

E

L

I

D

A

R

E

H

K

A

S

Q

R

-

I

-

F

-

G

-

V

-

E

-

L

-

N

-

V

-

I

A

S

L

I

A

T

A

D

R
|
R

T

S

G

G

Q

T

R

I

I

W

-

G

E

D

V

F

T

D

A

L

V

L

S

E

A

A

K

K

A

E

R

V

D

A

K

E

D

S

R

T

G

G

I

-

D

K

L

L

S

S

R

N

F

I

A

G

W

D

F

Y

D

E

-

V

-

Y

-

N

-

F

D

S

P

P

V

Q

A

E

L

L

A

V

A

E

S

E

P

V

D

K

I

P

D

N

V

I

F

L

V

V

E

D

L
x
V

I
x
S

G
x
S

G

W

E

D

E

E

G

-

A

-

A

A

R

H

A

E

A

M

V

Y

E

K

T

V

A

A

I

L

A

G

A

E

G

G

K

I

S

S

V

V

T

T

A
x
S

N
|
N

K
|
K

A

P

L

P

L

I

A

A

K

N

H

Y

G

Y

M

D

A

E

L

L

A

M

R

N

A

L

A

A

E

K

E

E

K

N

G

N

V

A

A

G

L

I

A

F

F

F

E

E

A
x
S

S

T

V

V

A

M

G

A

G

G

I

T

P

P

V

I

I

I

K

G

T

V

L

L

R

R

E

E

G

N

F

L

A

L

G

G

N

E

A

N

I

I

E

K

R

R

I

I

Y

D

G

A

I

V

L

V

N

N

G
x
A

T
x
S

C

T

N

T

Y

F

I

I

L

L

S

T

R

K

M

M

E

-

T

S

E

E

K

G

L

K

S

T

F

L

A

D

C

A

L

I

R

E

E

E

A

A

Q

K

E

S

L

L

G

G

Y

I

A

L

E

E

A

E

D

D

P

P

T

S

F

K

D
|
D

I

I

G

D

G

G

F

I

D
|
D

T

A

A

Y

H

Y

K
|
K

L

A

A

K

I

I

L

L

T

H

A

W

L

V

A

S

F

Y

G

G

T

E

T

P

I

-

D

-

P

P

D

E

A

-

I

-

A

-

I

-

E

-

G

-

I

-

E

E

T

E

I

E

T

R

L

L

A

G

D

I

L

R

E

E

A

V

A

R

D

D

E

A

L

R

G

N

Y

V

R

R

V

L

K

-

L

-

G

-

V

V

A

A

Q

Q

K

V

T

S

L

K

S

G

G

K

I

I

E

S

Q

-

R

-

V

V

H

K

P

P

T

R

M

K

V

L

P

S

K

S

S

D

S

N

A

P

I

L

A

L

Q

-

I

V

M

E

G

G

V

V

I

Q

N

N

A

A

V

A

S

V

I

I

N

R

A

T

D

N

A

N

V

L

H

G

E

E

L

V

T

I

L

L

A

K

G

G

P

P

G

G

A
x
G

G

G

E

R

A

V

T
|
T

A

A

S

S

A

G

V

V

V

F

A

T

D

D

L

I

active site: D211 (= D203), K215 (= K207)
binding l-homoserine: A171 (≠ G162), S172 (≠ T163), D206 (= D198), K215 (= K207)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F8 (≠ A10), F10 (≠ L12), G11 (= G13), T12 (= T14), V13 (= V15), R40 (= R33), V91 (≠ L80), S92 (≠ I81), S93 (≠ G82), S114 (≠ A105), N115 (= N106), K116 (= K107), S141 (≠ A132), G295 (≠ A303), T300 (= T308)

O81852 Bifunctional aspartokinase/homoserine dehydrogenase 2, chloroplastic; AK-HD 2; AK-HSDH 2; Beta-aspartyl phosphate homoserine 2; EC 2.7.2.4; EC 1.1.1.3 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
28% identity, 72% coverage: 5:320/437 of query aligns to 558:909/916 of O81852

query
sites
O81852

L

L

R

A

V

M

G

G

I

I

A

V

G

G

L

P

G

G

T

L

V

I

G

G

T

A

A

T

V

L

R

D

L

Q

L

L

D

R

R

D

H

Q

A

A

Q

A

A

V

L

L

A

K

A

Q

R

E

T

F

G

N

Q

I

R

D

I

L

E

R

V

V

T

L

A

G

V

I

-

T

-

G

S

S

A

K

K

K

A

M

R

L

D

L

K

S

D

D

R

I

G

G

I

I

D

D

L

L

S

S

R

R

F

-

A

-

W

W

F

R

D

E

D

L

P

L

V

N

A

E

L

K

A

G

A

T

S

E

P

A

D

D

I

L

D

D

V

K

F

F

V

T

E

Q

L

Q

I

V

G

H

G

G

E

N

E

H

G

F

A

I

A

P

R

N

A

S

A

V

V

V

-

V

-

D

-

C

-

T

-

A

E

D

T

S

A

A

I

I

A

A

A

S

-

R

-

Y

-

D

-

W

-

L

-

R

-

K

G

G

K

I

S

H

V

V

V

I

T

T

A

P

N

N

K

K

A

K

L

-

A

A

K

N

H

S

G

G

-

P

-

L

-

D

-

Q

-

Y

M

L

A

K

L

L

A

R

R

D

A

L

A

Q

E

R

E

K

K

S

G

Y

V

T

A

H

L

Y

A

F

F

Y

E

E

A

A

S

T

V

V

A

G

G

A

G

G

I

L

P

P

V

I

I

I

K

S

T

T

L

L

R

R

E

G

G

L

F

L

-

E

A

T

G

G

N

D

A

K

I

I

E

L

R

R

I

I

Y

E

G

G

I

I

L

C

N

S

G

G

T

T

C

L

N

S

Y

Y

I

L

L

F

S

N

R

N

M

F

E

V

T

G

E

D

K

R

L

-

S

S

F

F

A

S

A

E

C

V

L

V

R

T

E

E

A

A

Q

K

E

N

L

A

G

G

Y

F

A

T

E

E

A

P

D

D

P

P

T

R

F

D

D

D

I

L

G

S

G

G

F

T

D

D

T

V

A

A

H

R

K

K

L

V

A

I

I

I

L

L

T

-

A

A

L

R

A

E

F

S

G

G

T

L

T

K

I

L

D

D

P

L

D

A

A

D

I

L

A

P

I

I

-

R

-

S

-

L

-

V

-

P

-

E

-

P

-

L

-

K

-

G

-

C

-

T

-

S

-

V

-

E

E

E

G

F

I

M

E

E

T

K

I

L

T

P

L

Q

A

Y

D

D

L

G

E

D

A

L

A

A

D

K

E

E

L

R

-

L

-

D

-

A

-

E

-

N

-

S

G

G

Y

E

R

V

V

L

K

R

L

Y

L

V

G

G

V

V

A

-

Q

-

K

-

T

-

L

V

S

D

G

A

I

V

E

N

Q

Q

R

K

-

G

-

T

V

V

H

E

P

L

T

R

M

R

V

Y

P

K

K

K

S

E

S

H

A

P

I

F

A

A

Q

Q

I

L

M

A

G

G

V

S

I

D

N

N

A

I

V

I

S

A

I

F

N

T

A

T

D

T

A

R

V

Y

-

K

-

D

H

H

E

P

L

L

T

I

L

V

A

R

G

G

P

P

G

G

A

A

G

G

G

A

E

Q

A

V

T

T

A

A

S

G

A

G

V

I

V

F

A

S

D

D

L

I

A

L

D

R

L

L

A

A

Sites not aligning to the query:

441 I→A: Loss of threonine sensitivity for the aspartokinase activity and decreased inhibition of homoserine dehydrogenase activity by threonine.
443 Q→A: Loss of threonine sensitivity for the aspartokinase activity and decreased inhibition of homoserine dehydrogenase activity by threonine.
522 I→A: No effect on the inhibition of aspartokinase activity by threonine, but decreased inhibition of homoserine dehydrogenase activity by threonine.
524 Q→A: No effect on the inhibition of aspartokinase activity by threonine, but decreased inhibition of homoserine dehydrogenase activity by threonine.

3ingA Crystal structure of homoserine dehydrogenase (np_394635.1) from thermoplasma acidophilum at 1.95 a resolution
25% identity, 52% coverage: 3:231/437 of query aligns to 1:237/319 of 3ingA

query
sites
3ingA

R

K

H

E

L

I

R

R

V

I

G

I

I

L

A

M

G
|
G

L
x
T

G
|
G

T
x
N

V
|
V

G

G

T

L

A

N

V

V

V

L

R

R

L

I

D

D

R

A

H

S

-

N

-

R

-

F

-

S

-

I

-

K

-

V

-

G

-

V

A

S

Q
x
D

A
x
S

L

R

A

S

A

Y

R

A

T

S

G

G

Q

R

R

N

I

L

E

D

V

I

T

S

A

S

V

I

S

I

A

S

K

N

A
x
K

R

E

D

K

K

T

D

G

R

R

G

I

I

S

D

D

L

R

S

A

R

-

F

F

A

S

W

G

F

P

D

E

D

D

P

L

V

M

A

G

L

E

A

A

S

-

P

-

D

-

I

-

D

D

V

L

F

L

V

V

E

D

L
x
C

I
x
T

G
x
P

G

A

E

S

E

R

G

D

A

G

A

V

R

R

-

E

-

Y

A

S

A

L

V

Y

E

R

T

M

A

A

I

F

A

E

A

S

G

G

K

M

S

N

V

V

T

T

A
|
A

N
|
N

K
|
K

A

S

L

G

L

L

A

A

K

N

H

K

G

W

M

H

A

D

L

I

A

M

R

D

A

S

A

A

E

N

E

Q

K

N

G

S

V

K

A

Y

L

I

A

R

F

Y

E

E

A
|
A

S

T

V

V

A

A

G

G

I

V

P

P

V

L

I

F

K

S

T

V

L

L

R

D

E

Y

G

S

F

I

A

L

G

P

N

S

A

K

I

V

E

K

R

R

I

F

Y

R

G

G

I

I

L

V

N

S

G

S

T

T

C

I

N

N

Y

Y

I

V

L

I

S

R

R

N

M

M

E

A

T

N

E

G

K

R

L

-

S

S

F

L

A

R

A

D

C

V

L

V

R

D

E

D

A

A

Q

I

E

K

L

K

G

G

Y

I

A

A

E
|
E

A
x
S

D

N

P

P

T

Q

F

D

D

D

I

L

G

N

G

G

F

L

D
|
D

T

A

A

A

H

R

K
|
K

L

S

A

V

I

I

L

L

T

V

A

N

L

H

A

I

F

F

G

G

T

T

T

E

I

Y

D

T

P

L

D

N

A

D

I

V

A

E

I

Y

E

S

G

G

I

V

E

D

active site: D209 (= D203), K213 (= K207)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G9 (= G11), T10 (≠ L12), G11 (= G13), N12 (≠ T14), V13 (= V15), D38 (≠ Q28), S39 (≠ A29), K57 (≠ A47), C85 (≠ L80), T86 (≠ I81), P87 (≠ G82), A112 (= A105), N113 (= N106), K114 (= K107), A139 (= A132), E198 (= E192), S199 (≠ A193)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 302, 305

P31116 Homoserine dehydrogenase; HDH; HSD; EC 1.1.1.3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
29% identity, 34% coverage: 96:244/437 of query aligns to 106:260/359 of P31116

query
sites
P31116

I

V

A

E

A

N

G

G

K

I

S

S

V

I

V

A

T

T

A

P

N

N

K

K

A

K

L

A

L

F

A

S

K

S

H

D

G

-

M

L

A

A

L

T

A

W

R

K

A

A

A

L

E

F

E

S

K

N

G

K

V

P

A

T

L

N

A

G

F

F

-

V

-

Y

-

H

E
|
E

A

A

S

T

V
|
V

A

G

G
x
A

G

G

I
x
L

P

P

V

I

I

I

K

S

T

F

L

L

R

R

E

E

G

I

F

I

-

Q

A

T

G

G

N

D

A

E

I

V

E

E

R

K

I

I

Y

E

G

G

I

I

L

F

N

S

G

G

T

T

C

L

N

S

Y

Y

I

I

L

F

S

N

R

E

M

F

E

S

T

T

E

S

K

Q

-

A

-

N

-

D

L

V

S

K

F

F

A

S

A

D

C

V

L

V

R

K

E

V

A

A

Q

K

E

K

L

L

G

G

Y

Y

A

T

E
|
E

A

P

D

D

P

P

T

R

F

D

D

D

I

L

G

N

G

G

F

L

D
|
D

T

V

A

A

H

R

K
|
K

L

V

A

T

I

I

L

V

T

G

A

R

L

I

A

S

F

G

G

V

T

E

T

V

I

E

D

S

P

P

D

T

A

S

I

F

A

P

I

V

E

Q

G

S

I

L

E

I

T

P

I

K

T

P

L

L

A

E

D

S

L

V

E

K

A

S

A

A

D

D

E

E

L

F

E143 (= E131) binding Na(+)
V146 (= V134) binding Na(+)
A148 (≠ G136) binding Na(+)
L150 (≠ I138) binding Na(+)
E208 (= E192) binding L-homoserine; mutation to D: Increases KM for aspartate-semialdehyde 48-fold and reduces kcat by 50%.; mutation E->L,Q: Loss of activity.
D219 (= D203) binding L-homoserine; mutation to L: Reduces kcat 150-fold.
K223 (= K207) mutation to V: Loss of activity.

Sites not aligning to the query:

13 binding NAD(+)
15 binding NAD(+)
16 binding NAD(+)
41 binding NAD(+)
79 H→A: Reduces kcat 2-fold.
93 binding NAD(+)
309 H→A: Reduces kcat 40-fold. Affects dimer formation.
340 binding NAD(+)

1tveA Homoserine dehydrogenase in complex with 4-(4-hydroxy-3- isopropylphenylthio)-2-isopropylphenol (see paper)
29% identity, 34% coverage: 96:244/437 of query aligns to 105:259/358 of 1tveA

query
sites
1tveA

I

V

A

E

A

N

G

G

K

I

S

S

V

I

V

A

T

T

A

P

N

N

K
|
K

A

K

L

A

L

F

A

S

K

S

H

D

G

-

M

L

A

A

L

T

A

W

R

K

A

A

A

L

E

F

E

S

K

N

G

K

V

P

A

T

L

N

A

G

F

F

-

V

-

Y

-

H

E

E

A

A

S

T

V

V

A

G

G

A

G

G

I

L

P

P

V

I

I

I

K

S

T

F

L

L

R

R

E

E

G

I

F

I

-

Q

A

T

G

G

N

D

A

E

I

V

E

E

R

K

I

I

Y

E

G

G

I

I

L

F

N

S

G

G

T
|
T

C

L

N

S

Y

Y

I

I

L

F

S

N

R

E

M

F

E

S

T

T

E

S

K

Q

-

A

-

N

-

D

L

V

S

K

F

F

A

S

A

D

C

V

L

V

R

K

E

V

A

A

Q

K

E

K

L

L

G

G

Y

Y

A

T

E

E

A

P

D

D

P

P

T

R

F

D

D
|
D

I

L

G

N

G

G

F

L

D
|
D

T

V

A

A

H

R

K
|
K

L

V

A

T

I

I

L

V

T

G

A

R

L

I

A

S

F

G

G

V

T

E

T

V

I

E

D

S

P

P

D

T

A

S

I

F

A

P

I

V

E

Q

G

S

I

L

E

I

T

P

I

K

T

P

L

L

A

E

D

S

L

V

E

K

A

S

A

A

D

D

E

E

L

F

active site: D218 (= D203), K222 (= K207)
binding 4-(4-hydroxy-3-isopropylphenylthio)-2-isopropylphenol: K116 (= K107), T175 (= T163), D213 (= D198), D218 (= D203)

Sites not aligning to the query:

binding 4-(4-hydroxy-3-isopropylphenylthio)-2-isopropylphenol: 93

1q7gA Homoserine dehydrogenase in complex with suicide inhibitor complex NAD-5-hydroxy-4-oxonorvaline (see paper)
29% identity, 34% coverage: 96:244/437 of query aligns to 105:259/358 of 1q7gA

query
sites
1q7gA

I

V

A

E

A

N

G

G

K

I

S

S

V

I

V

A

T

T

A
x
P

N

N

K
|
K

A

K

L

A

L

F

A

S

K

S

H

D

G

-

M

L

A

A

L

T

A

W

R

K

A

A

A

L

E

F

E

S

K

N

G

K

V

P

A

T

L

N

A

G

F

F

-

V

-

Y

-

H

E

E

A
|
A

S

T

V

V

A

G

G

A

G

G

I

L

P

P

V

I

I

I

K

S

T

F

L

L

R

R

E

E

G

I

F

I

-

Q

A

T

G

G

N

D

A

E

I

V

E

E

R

K

I

I

Y

E

G

G

I

I

L

F

N
x
S

G

G

T

T

C

L

N

S

Y

Y

I

I

L

F

S

N

R

E

M

F

E

S

T

T

E

S

K

Q

-

A

-

N

-

D

L

V

S

K

F

F

A

S

A

D

C

V

L

V

R

K

E

V

A

A

Q

K

E

K

L

L

G

G

Y

Y

A

T

E

E

A

P

D

D

P

P

T

R

F

D

D

D

I

L

G

N

G

G

F

L

D
|
D

T

V

A

A

H

R

K
|
K

L

V

A

T

I

I

L

V

T

G

A

R

L

I

A

S

F

G

G

V

T

E

T

V

I

E

D

S

P

P

D

T

A

S

I

F

A

P

I

V

E

Q

G

S

I

L

E

I

T

P

I

K

T

P

L

L

A

E

D

S

L

V

E

K

A

S

A

A

D

D

E

E

L

F

active site: D218 (= D203), K222 (= K207)
binding nicotinamide-adenine-dinucleotide-5-hydroxy-4-oxonorvaline: P114 (≠ A105), K116 (= K107), A143 (= A132), S173 (≠ N161), K222 (= K207)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide-5-hydroxy-4-oxonorvaline: 13, 14, 15, 39, 91, 92, 93, 97, 338, 343

1ebuD Homoserine dehydrogenase complex with NAD analogue and l-homoserine (see paper)
29% identity, 34% coverage: 96:244/437 of query aligns to 105:259/358 of 1ebuD

query
sites
1ebuD

I

V

A

E

A

N

G

G

K

I

S

S

V

I

V

A

T

T

A

P

N

N

K
|
K

A

K

L

A

L

F

A

S

K

S

H

D

G

-

M

L

A

A

L

T

A

W

R

K

A

A

A

L

E

F

E

S

K

N

G

K

V

P

A

T

L

N

A

G

F

F

-

V

-

Y

-

H

E

E

A

A

S

T

V

V

A

G

G

A

G

G

I

L

P

P

V

I

I

I

K

S

T

F

L

L

R

R

E

E

G

I

F

I

-

Q

A

T

G

G

N

D

A

E

I

V

E

E

R

K

I

I

Y

E

G

G

I

I

L

F

N

S

G

G

T

T

C

L

N

S

Y

Y

I

I

L

F

S

N

R

E

M

F

E

S

T

T

E

S

K

Q

-

A

-

N

-

D

L

V

S

K

F

F

A

S

A

D

C

V

L

V

R

K

E

V

A

A

Q

K

E

K

L

L

G

G

Y

Y

A

T

E

E

A

P

D

D

P

P

T

R

F

D

D

D

I

L

G

N

G

G

F

L

D
|
D

T

V

A

A

H

R

K
|
K

L

V

A

T

I

I

L

V

T

G

A

R

L

I

A

S

F

G

G

V

T

E

T

V

I

E

D

S

P

P

D

T

A

S

I

F

A

P

I

V

E

Q

G

S

I

L

E

I

T

P

I

K

T

P

L

L

A

E

D

S

L

V

E

K

A

S

A

A

D

D

E

E

L

F

active site: D218 (= D203), K222 (= K207)
binding 3-aminomethyl-pyridinium-adenine-dinucleotide: K116 (= K107)

Sites not aligning to the query:

binding 3-aminomethyl-pyridinium-adenine-dinucleotide: 11, 12, 13, 14, 15, 39, 40, 91, 93, 343

1ebfA Homoserine dehydrogenase from s. Cerevisiae complex with NAD+ (see paper)
29% identity, 34% coverage: 96:244/437 of query aligns to 105:259/358 of 1ebfA

query
sites
1ebfA

I

V

A

E

A

N

G

G

K

I

S

S

V

I

V

A

T

T

A
x
P

N

N

K

K

A

K

L

A

L

F

A

S

K

S

H

D

G

-

M

L

A

A

L

T

A

W

R

K

A

A

A

L

E

F

E

S

K

N

G

K

V

P

A

T

L

N

A

G

F

F

-

V

-

Y

-

H

E

E

A

A

S

T

V

V

A

G

G

A

G

G

I

L

P

P

V

I

I

I

K

S

T

F

L

L

R

R

E

E

G

I

F

I

-

Q

A

T

G

G

N

D

A

E

I

V

E

E

R

K

I

I

Y

E

G

G

I

I

L

F

N

S

G

G

T

T

C

L

N

S

Y

Y

I

I

L

F

S

N

R

E

M

F

E

S

T

T

E

S

K

Q

-

A

-

N

-

D

L

V

S

K

F

F

A

S

A

D

C

V

L

V

R

K

E

V

A

A

Q

K

E

K

L

L

G

G

Y

Y

A

T

E

E

A

P

D

D

P

P

T

R

F

D

D

D

I

L

G

N

G

G

F

L

D
|
D

T

V

A

A

H

R

K
|
K

L

V

A

T

I

I

L

V

T

G

A

R

L

I

A

S

F

G

G

V

T

E

T

V

I

E

D

S

P

P

D

T

A

S

I

F

A

P

I

V

E

Q

G

S

I

L

E

I

T

P

I

K

T

P

L

L

A

E

D

S

L

V

E

K

A

S

A

A

D

D

E

E

L

F

active site: D218 (= D203), K222 (= K207)
binding nicotinamide-adenine-dinucleotide: P114 (≠ A105)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 10, 12, 13, 14, 15, 39, 40, 92, 93

O94671 Homoserine dehydrogenase; HDH; HSD; EC 1.1.1.3 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
39% identity, 22% coverage: 119:215/437 of query aligns to 138:239/376 of O94671

query
sites
O94671

R

K

A

A

A

S

E

K

E

E

K

S

G

G

V

A

A

L

L

L

A

M

F

H

E

E

A

A

S

S

V

V

A

G

G

A

G

G

I

L

P

P

V

I

I

I

K

S

T

T

L

L

R

K

E

E

G

L

F

I

A

A

-

T

G

G

N

D

A

E

I

I

E

I

R

K

I

I

Y

E

G

G

I

I

L

F

N

S

G

G

T

T

C

L

N

S

Y

Y

I

I

L

F

S

N

R

V

M

W

E

S

T

P

E

N

-

G

-

K

-

G

K

T

L

A

S
|
S

F

F

A

S

A

D

C

I

L

V

R

K

E

I

A

A

Q

K

E

Q

L

N

G

G

Y

Y

A

T

E

E

A

P

D

D

P

P

T

R

F

D

D

D

I

L

G

N

G

G

F

M

D

D

T

V

A

A

H

R

K

K

L

V

A

T

I

I

L

L

T

S

A

R

L

I

A

A

S201 (= S177) modified: Phosphoserine

Query Sequence

>WP_026595805.1 NCBI__GCF_000385335.1:WP_026595805.1
MMRHLRVGIAGLGTVGTAVVRLLDRHAQALAARTGQRIEVTAVSAKARDKDRGIDLSRFA
WFDDPVALAASPDIDVFVELIGGEEGAARAAVETAIAAGKSVVTANKALLAKHGMALARA
AEEKGVALAFEASVAGGIPVIKTLREGFAGNAIERIYGILNGTCNYILSRMETEKLSFAA
CLREAQELGYAEADPTFDIGGFDTAHKLAILTALAFGTTIDPDAIAIEGIETITLADLEA
ADELGYRVKLLGVAQKTLSGIEQRVHPTMVPKSSAIAQIMGVINAVSINADAVHELTLAG
PGAGGEATASAVVADLADLARGSHIAPFGLPVADLAPLQRAPLQMHEGGYYVRLSVHNRP
GAAAAIATRMAENGISLESIVQRNVSGSRAKAATLDPVPVVLITYATYEKTIRQALESVV
ADGYLAEKPQLIRIERE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory